Techno-functional properties and antioxidant capacity of the concentrate and protein fractions of Leucaena spp. seeds / Propiedades tecno-funcionales y capacidad antioxidante del concentrado y fracciones proteicas de semillas de Leucaena spp

Introduction: The use of vegetable proteins as ingredients in food systems is based on their functional properties. The water and oil holding capacity, foaming, and emulsifying capacity/stability, and antioxidant assay of the protein fractions - albumins, globulins 7S/11S, glutelins and prolamins - isolated from Leucaena seed were evaluated. Objective: The objective of this study was to evaluate the functional properties and antioxidant capacity of the concentrate and protein fractions of ripe Leucaena spp. seeds. Materials and methods: Ripe Leucaena seeds were collected and evaluated in Oaxaca, Mexico (16°59'21''N 96°43'26''O) during the months of February-April 2021.The protein concentrate was isolated by isoelectric precipitation (pH=9, pH=4). The albumins, globulins, glutelins and prolamins were isolated based on their solubility properties in different extracting solutions. Results: Glutelins constituted the main protein fraction (75.88%). Prolamins were not found. The glutelins fractions showed the highest oil holding capacity (0.93±0.08 mL g-1). The albumins fraction had the highest water holding capacity (2.53±0.15 mL g-1), foaming capacity and foam stability (71.83±1.26 % and 70.00±0.00%, respectively) and antioxidant capacity (18.09±0.88%). The globulins exhibited the highest emulsifying capacity and emulsion stability (56.83±1.76% and 55.67±1.20%, respectively). Conclusions: The concentrate and protein fraction of Leucaena seeds showed different techno-functional and antioxidant properties of interest for the food industry, like those showed by other commercial vegetable proteins(AU)

Introducción: El uso de proteínas vegetales como ingredientes en sistemas alimentarios se basa en sus propiedades funcionales. Se evaluó la capacidad de retención de agua y aceite, la capacidad/estabilidad espumante y emulsionante y el ensayo antioxidante de las fracciones proteicas -albúminas, globulinas 7S/11S, glutelinas y prolaminas- aisladas de las semillas de Leucaena. Objetivo: El objetivo de este estudio fue evaluar las propiedades funcionales y la capacidad antioxidante del concentrado y las fracciones proteicas de las semillas maduras de Leucaena spp. Materiales y métodos: Las semillas maduras de Leucaena fueron recolectadas y evaluadas en Oaxaca, México (16°59'21''N 96°43'26''O) durante los meses de febrero-abril del año 2021. Se usó harina de Leucaena desgrasada para la preparación de las fracciones proteicas. El concentrado proteico se aisló por precipitación isoeléctrica (pH=9, pH=4). Las albúminas, globulinas, glutelinas y prolaminas se aislaron en función de sus propiedades de solubilidad en diferentes soluciones de extracción. Resultados: Las glutelinas constituyeron la principal fracción proteica (75,88%). No se encontraron prolaminas. La fracción de glutelinas mostró la mayor capacidad de retención de aceite (0.93±0,08 mL g-1). La fracción de albúminas presentó la mayor capacidad de retención de agua (2,53±0,15 mL g-1), capacidad espumante y estabilidad de la espuma (71,83±1,26% y 70,00±0,00%, respectivamente) y capacidad antioxidante (18,09±0,88%). Las globulinas mostraron la mayor capacidad emulsionante y estabilidad de la emulsión (56,83±1,76 y 55,67±1,20%, respectivamente). Conclusiones: El concentrado y las fracciones proteicas de las semillas de Leucaena mostraron diferentes propiedades tecno-funcionales y antioxidantes de interés para la industria alimentaria, similares a los reportados por diversas proteínas vegetales comerciales(AU)

Barreras y facilitadores para la formulación de políticas públicas que regulan los ambientes alimentarios obesogénicos: revisión de la literatura / Barriers and facilitators to policymaking public policies that regulate obesogenic food environments: review of the literature

INTRODUCCIÓN: Los ambientes alimentarios determinan en gran medida el comportamiento alimentario de la población, por lo que se asocian a la epidemia de obesidad. Es responsabilidad de los Estados formular e implementar políticas para intervenir los ambientes alimentarios obesogénicos. El éxito de las políticas dependerá de factores que actúan como barreras o facilitadores. OBJETIVO: Identificar las barreras y facilitadores que se presentan al formular e implementar políticas públicas que aborden los ambientes alimentarios obesogénicos. MATERIAL Y MÉTODO: Estudio secundario en base a revisión narrativa. La identificación, tamizaje y selección de los artículos publicados se guio por el diagrama PRISMA. Para la identificación se recurrió a las bases de datos electrónicas PubMed, Scielo, Scopus y Web of Science (WOS), ingresando campos de búsqueda y palabras clave. En el tamizaje se eliminaron los duplicados y se aplicaron criterios de inclusión. La calidad metodológica de los estudios elegibles se evaluó con el protocolo CASPe. RESULTADOS: Se revisaron estudios cualitativos que usaron entrevistas a actores claves. Las barreras identificadas para el proceso de formulación de políticas de ambientes alimentarios obesogénicos son el impacto económico negativo, falta de evidencia científica sobre la efectividad de las políticas, influencia de la industria alimentaria, debilidades de coordinación y cooperación técnica. Los facilitadores identificados son el compromiso político de los Estados con las agencias internacionales, enfoque de trabajo conjunto con la industria alimentaria y el apoyo académico. CONCLUSIÓN: La formulación de políticas para intervenir ambientes alimentarios obesogénicos está especialmente limitada por la industria alimentaria. Es relevante generar evidencia científica del impacto que, las regulaciones implementadas, han tenido en los factores económicos y sobre la salud de la población. La colaboración entre gobiernos e industria alimentaria, podría derivar políticas que, además de impactar positivamente la salud de la población, sean una oportunidad para el sector privado y la generación de empleo.

INTRODUCTION: Food environments largely determine the eating behavior of the population, which is why they are associated with the obesity epidemic. It is the responsibility of States to formulate and implement policies to intervene in obesogenic food environments. The success of the policies will depend on factors that act as barriers or facilitators. OBJECTIVE: To identify the barriers and facilitators for the formulation and implementation of public policies that address obesogenic food environments. MATERIAL AND METHOD: Secondary study based on narrative review. The identification, screening and selection of published articles was guided by the PRISMA diagram. For the identification, the electronic databases PubMed, Scielo, Scopus and Web of Science (WOS) were used, entering search fields and keywords. In the screening, duplicates were eliminated and inclusion criteria were applied. The methodological quality of eligible studies was assessed using the CASPe protocol. RESULTS: Qualitative studies that used interviews with key actors were reviewed. The barriers identified for the process of formulating policies on obesogenic food environments are the negative economic impact, lack of scientific evidence on the effectiveness of policies, influence of the food industry, weaknesses in coordination and technical cooperation. The identified facilitators are the political commitment of the States with international agencies, a joint work approach with the food industry and academic support. CONCLUSION: Policymaking to intervene in obesogenic food environments is especially limited by the food industry. It is relevant to generate scientific evidence of the impact that the implemented regulations have had on economic factors and on the health of the population. Collaboration between governments and the food industry could derive policies that, in addition to having a positive impact on the health of the population, would mean an opportunity for the private sector and the generation of employment.

Harassment of health professionals by the infant food industry at scientific events / Assédio da indústria de alimentos infantis a profissionais de saúde em eventos científicos

ABSTRACT OBJECTIVE To analyze the receipt of sponsorships from breast-milk substitute companies by health professionals in scientific events. METHODS Multicenter study (Multi-NBCAL) performed from November 2018 to November 2019 in six cities in different Brazilian regions. In 26 public and private hospitals, pediatricians, nutritionists, speech therapists, and a hospital manager were interviewed using a structured questionnaire. Descriptive analyses were carried out regarding the health professionals' knowledge about the Norma Brasileira de Comercialização de Alimentos para Lactentes e Crianças de Primeira Infância, Bicos, Chupetas e Mamadeiras (NBCAL - Brazilian Code of Marketing of Infant and Toddlers Food and Childcare-related Products), companies sponsoring scientific events, and material or financial sponsorships received, according to profession. RESULTS We interviewed 217 health professionals, mainly pediatricians (48.8%). Slightly more than half of the professionals (54.4%) knew NBCAL, most from Baby-friendly Hospitals. Most health professionals (85.7%) attended scientific events in the last two years, more than half of them (54.3%) sponsored by breast-milk substitute companies, especially Nestlé (85.1%) and Danone (65.3%). These professionals received sponsorships in the events, such as office supplies (49.5%), meals or invitations to parties (29.9%), promotional gifts (21.6%), payment of the conference registration fee (6.2%) or ticket to the conference (2.1%). CONCLUSION The infant food industries violate NBCAL by harassing health professionals in scientific conferences, offering diverse material and financial sponsorships.

RESUMO OBJETIVO Analisar o recebimento de patrocínios da indústria de substitutos do leite materno por profissionais de saúde em eventos científicos. MÉTODOS Inquérito multicêntrico (Multi-NBCAL) conduzido entre novembro de 2018 e novembro de 2019 em seis cidades de diferentes regiões brasileiras. Em 26 hospitais públicos e privados foram entrevistados pediatras, nutricionistas, fonoaudiólogos e um membro da chefia, mediante questionário estruturado. Foram realizadas análises descritivas do conhecimento dos profissionais de saúde sobre a Norma Brasileira de Comercialização de Alimentos para Lactentes e Crianças de Primeira Infância, Bicos, Chupetas e Mamadeiras (NBCAL), das empresas patrocinadoras de eventos científicos e dos patrocínios financeiros ou materiais recebidos, conforme a categoria profissional. RESULTADOS Foram entrevistados 217 profissionais de saúde, principalmente pediatras (48,8%). Pouco mais da metade dos profissionais (54,4%) afirmaram conhecer a NBCAL, principalmente em Hospitais Amigos da Criança. A maior parte (85,7%) dos profissionais de saúde havia participado de congressos científicos nos últimos dois anos, mais da metade, 54,3%, deles apoiados pela indústria de substitutos do leite materno, em especial pela Nestlé (85,1%) e Danone (65,3%). Patrocínios foram recebidos por esses profissionais nos eventos, como materiais de escritório (49,5%), refeições ou convites para festas (29,9%), brindes (21,6%), pagamento de inscrição (6,2%) ou de passagem para o congresso (2,1%). CONCLUSÃO As indústrias de alimentos infantis infringem a NBCAL ao assediar profissionais de saúde em congressos científicos, oferecendo patrocínios materiais e financeiros diversos.

Higienización y eliminación fúngica en instalaciones industriales para la disminución de riesgos ocupacionales / Sanitization and fungal elimination in industrial facilities to reduce occupational risks

Se ha comprobado que la exposición a bioaerosoles se asocia con varios efectos sobre la salud, como enfermedades pulmonares y alergias. El presente estudio transversal tuvo como objetivo investigar la contaminación por hongos en varias superficies pertenecientes a dos industrias de alimentos. La toma de muestra se realizó en tres semanas, inmediatamente después de realizada la limpieza y desinfección y antes de iniciar la producción. Se recolectaron 400 muestras de superficie (vidrio, acero inoxidable, aluminio, goma y plástico), también se evaluó la eficacia del programa de higienización en el control de la población de hongos. Todas las muestras presentaron recuentos fúngicos <10 ufc/cm2, sin haber diferencias significativas entre los tipos de superficie, sin embargo, las superficies plásticas exhibieron mayor crecimiento logarítmico de los hongos. Se determinó que 49,60% de las especies identificadas correspondieron al género Penicillium. Aunque los niveles de hongos en las industrias estudiadas fueron inferiores a los niveles recomendados por la Organización Mundial de la Salud, algunas medidas de salud ambiental como lavar y desinfectar las superficies después de cada turno de trabajo, y se recomiendan inspecciones periódicas para garantizar la seguridad de los trabajadores y de los productos que allí se manufacturan(AU)

It has been proven that exposure to bioaerosols is associated with several health effects, such as pulmonary diseases and allergies. The present crosssectional study aimed to investigate fungal contamination on various surfaces belonging to two food industries. The sampling was carried out in three weeks, immediately after cleaning and disinfection and before starting production. 400 surface samples were collected (glass, stainless steel, aluminum, rubber and plastic), the effectiveness of the sanitation program in controlling the fungal population was also evaluated. All the samples presented fungal counts <10 cfu / cm2, with no significant differences between the types of surfaces, however, the plastic surfaces exhibited higher logarithmic growth of the fungi. It was determined that 49.60% of the identified species corresponded to the Penicillium genus.Although the levels of fungi in the studied baths were lower than the levels recommended by the World Health Organization, some environmental health measure ssuch as washing and disinfecting surfaces after each working shift and periodic inspections are recommended ensuring the safety of the workers and the products that are manufactured there(AU)

Actividad antioxidante y quelante de sistemas hidrocoloides mixtos formados por hidrolizados proteicos de Phaseolus vulgaris endurecido y goma modificada de flamboyán (Delonix regia) / Antioxidant and metal-chelating activities of mixed hydrocolloid systems formed by hard-to-cook Phaseolus vulgaris protein hydrolysates and flamboyant (Delonix regia) modified gum

Las proteínas y polisacáridos con frecuencia son utilizados simultáneamente en la industria de alimentos. Las interacciones entre ambos biopolímeros juegan un papel importante en la estructura y estabilidad de muchos alimentos procesados ya que pueden resultar en un sistema con propiedades bioactivas diferentes como ocurre en el caso de las funcionales. Objetivo. Evaluar los cambios en la capacidad antioxidante de un sistema hidrocoloide mixto formado por un hidrolizado enzimático proteico de frijol endurecido (P. vulgaris) y goma modificada de flamboyán (Delonix regia) (SHM). Materiales y métodos. El estudio se hizo entre febrero-octubre, 2014, en Mérida, México. Se modificó enzimáticamente el concentrado proteico de P. vulgaris con Pepsina-Pancreatina® y la goma extraída del flamboyán mediante carboximetilación, preparando dispersiones al 1% de cada uno de los biopolímeros, determinado la fluorescencia intrínseca de Trp (FIT) como indicador de la interacción entre ambos biopolímeros y la actividad antioxidante y quelante del sistema resultante. Resultados. Se obtuvo un hidrolizado proteico extensivo con 28,8% de grado de hidrólisis y una goma de flamboyán con grado de sustitución de 0,91. La mayor interacción entre ambos biopolímeros, se obtuvo empleando una relación 5:1 del SHM a pH 4 de acuerdo con el valor de FIT. Conclusiones. Los sistemas hidrocoloides mixtos preparados con hidrolizado extensivo de las proteínas Phaseolus vulgaris y goma modificada de flamboyán presentaron un incremento en la actividad antioxidante, respecto del hidrolizado dependiendo del mecanismo de oxidación, así como de las condiciones de pH en que se encuentra el sistema y la interacción entre ambos componentes(AU)

Proteins and polysaccharides are frequently used simultaneously in the food industry. The interactions between both biopolymers play an important role in the structure and stability of many processed foods since they can result in a system with different bioactive properties as in the case of functional ones. Objective. Evaluate the changes in the antioxidant capacity of mixed hydrocolloid system formed by a hard to cook bean (P. vulgaris) protein enzymatic hydrolyzate and modified flamboyant gum (Delonix regia) (SHM). Materials and methods. The study was conducted from February to October 2014 in Merida, Mexico. For this, the protein concentrate of P. vulgaris was treated with Pepsin-Pancreatin® and the gum extracted from the flamboyant were modified enzymatically by carboxymethylation, preparing 1% dispersions of each of the biopolymers, determining the intrinsic Trp fluorescence (FIT) as an indicator of the interaction between both biopolymers and the antioxidant and chelating activity of the resulting system. Results. The main results indicated that an extensive protein hydrolyzate with 28.8% degree of hydrolysis and a flamboyant gum with a substitution degree of 0.91 were obtained. The greatest interaction between both biopolymers was obtained using a 5:1 ratio of SHM to pH 4 according to the FIT value. Conclusions. The mixed hydrocolloid systems prepared with extensive hydrolyzate of the hard to cook P. vulgaris and modified flamboyant gum proteins showed an increase in antioxidant activity, compared to the hydrolyzate depending on the oxidation mechanism, as well as the pH conditions used and interaction between both component(AU)

Corporate Social Responsibility in the restaurant and fast food industry: a study of communication on healthy eating through social networks / Responsabilidade Social Corporativa no setor de restaurantes e fast food: um estudo da comunicação sobre alimentação saudável por meio de redes sociais / Responsabilidad Social Corporativa en el sector restauración y fast food: estudio de la comunicación sobre alimentación saludable en redes sociales

The communication of Corporate Social Responsibility (CSR) of a sample of eight restaurant and fast food companies is analyzed. An exploratory and descriptive method, based on the content analysis technique, is used to analyze the formal and informal CSR communication channels used to promote healthy eating on their respective websites and Twitter profiles. This study is complemented by a survey (n = 383) applied to a sample of young Spaniards to investigate their interest in healthy eating information, their assessment of CSR actions carried out in this area as well as the communication channels used for this purpose. The results confirm that the CSR policies of the companies include actions aimed at guaranteeing and promoting healthy eating. It is concluded about the convenience of using their own social networks and influencers to encourage healthy eating habits more effectively in their publics. (AU)

É analisada a comunicação de Responsabilidade Social Corporativa (RSC) de uma amostra de oito empresas de restaurantes e fast food. Um método exploratório e descritivo, baseado na técnica de análise de conteúdo é usado para analisar os canais de comunicação formais e informais de RSC usados para promover uma alimentação saudável nos seus respectivos sites e perfis no Twitter. Este estudo é complementado por uma pesquisa para descobrir o interesse na alimentação dos jovens espanhóis (n = 383), sua avaliação da RSC nessa área e os canais de informação usados para esse fim. Os resultados verificam a integração em suas políticas de RSE de ações relacionadas à promoção de hábitos alimentares saudáveis. Conclui-se sobre a conveniência de utilizar redes sociais próprias e influenciadores para estimular esse tipo de hábitos, dentro de suas políticas de RSE de maneira mais eficaz. (AU)

Se analiza la comunicación de la Responsabilidad Social Corporativa (RSC) de una muestra de ocho empresas de restauración y fast food. Se sigue una metodología exploratoria y descriptiva para analizar mediante un análisis de contenido la comunicación formal e informal de RSC respecto de la promoción de una alimentación saludable en sus respectivas webs y perfiles en Twitter. Este estudio se complementa con una encuesta (n = 383) sobre el interés por la alimentación de los jóvenes españoles, su valoración de la RSC en este ámbito y los canales de información utilizados al respecto. Los resultados verifican la integración en sus políticas de RSC de acciones relativas a la promoción de hábitos alimenticios saludables. Se concluye acerca de la conveniencia de uso de sus propias redes sociales y de influencers, para incentivar este tipo de hábitos, dentro de sus políticas de RSC. (AU)

Nanoemulsion Improves Babassu Palm Oil (Orbignya phalerata) Antioxidant Properties

HIGHLIGHTS Production of lipid nanoemulsions (<100 nm) of industrial interest with low energy demand. The antioxidant properties of babassu oil have been improved and the nanoemulsions are not cytotoxic. Babassu oil is a food and medicinal product. The nanoemulsion is strategic for the developed of new antioxidants phytotherapeutics.

Abstract Background: Babassu oil is an extract from a Brazilian native coconut (Orbignya phalerata Martius) and is used both as a food and a medicinal product. Methods: we produced two babassu oil nanoemulsions and evaluated them regarding their nanoscopic stability, antioxidant activity and cytotoxicity.The nanoemulsions were characterized by Dynamic Light Scattering, and their stability was investigated for 120 days. The antioxidant activity was assessed by Spectroscopy Electron Paramagnetic Resonance, and the cytotoxicity was assessed by a colorimetric method (MTT) with the NIH/3T3 cell lineage. Results: the results showed nanoemulsions with average hydrodynamic diameter lower than 100 nm (p(0.001).and a polydispersity index of less than 0.3 (p(0.001), indicating monodisperse systems and good stability at room temperature. The exposure of nanoemulsions at varying pH revealed that the isoelectric point was at 3.0, and the images obtained by Transmission Electron Microscopy showed spherical droplets with a size 27 nm. The antioxidant activity showed that the babassu nanoemulsions exposed to free radicals had a better response when compared to the oil free samples. The cell viability assays showed low toxicity of the formulation with viability over 92% (p(0.05). Conclusion: babassu oil nanoformulations showed low polydispersity and kinetic stability with effective antioxidant action. Therefore, they can be promising for application in the food industry or as antioxidant phytotherapeutics.

Effects of site-directed PEGylation on L-asparaginase thermostability / Efeitos da PEGuilação sítio-dirigida na termoestabilidade da L-asparaginase

The enzyme L-asparaginase (ASNase) is broadly applied as a drug to treat acute lymphoblastic leukemia, as well as in the food industry to avoid acrylamide formation in baked and fried food. In the present work, ASNase was covalently attached to polyethylene glycol (PEG) of different molecular weights (ASNase-PEG-5, ASNase-PEG-10, ASNase-PEG-20, and ASNase-PEG-40) at the N-terminal portion (monoPEGylation). Native and PEGylated forms were analyzed regarding thermodynamics and thermostability based on enzyme activity measurements. ASNase (native and PEGylated) presented maximum activity at 40 °C and denaturation followed a first-order kinetics. Based on these results, the activation energy for denaturation (E*d) was estimated and higher values were observed for PEGylated forms compared to the native ASNase, highlighting the ASNase-PEG10 with a 4.24-fold increase (48.85 kJ.mol-1) in comparison to the native form (11.52 kJ.mol-1). The enzymes were evaluated by residual activity over time (21 days) under different storage temperatures (4 and 37 °C) and the PEGylated conjugates remained stable after the 21 days. Thermodynamic parameters like enthalpy (ΔH‡), entropy (ΔS‡) and Gibbs free energy (ΔG‡) of ASNase (native and PEGylated) irreversible denaturation were also investigated. Higher - and positive - values of Gibbs free energy were found for the PEGylated conjugates (61.21 a 63.45 kJ.mol-1), indicating that the process of denaturation was not spontaneous. Enthalpy also was higher for PEGylated conjugates (18.84 a 46.08 kJ.mol-1), demonstrating the protective role of PEGylation. As for entropy, the negative values were more elevated for native ASNase (-0.149 J/mol.K), pointing out that the denaturation process enhanced the randomness and aggregation of the system, which was observed by circular dichroism. Thus, PEGylation proved its potential to increase ASNase thermostability

A enzima L-asparaginase (ASNase) é amplamente usada como medicamento para tratamento da leucemia linfoblástica aguda, bem como na indústria de alimentos para evitar a formação de acrilamida em alimentos cozidos e fritos. No presente trabalho, ASNase foi covalentemente ligada ao polímero poli(etilenoglicol) (PEG) de diferentes massas moleculares (ASNase-PEG-5, ASNase-PEG- 10, ASNase-PEG-20, and ASNase-PEG-40) na região N-terminal (monoPEGuilação) a fim de se estudar os efeitos da PEGuilação na termoestabilidade da enzima. As formas PEGuiladas e nativa foram analisadas em relação à termodinâmica e termoestabilidade a partir de atividade enzimática. A ASNase (nativa e PEGuilada) apresentou atividade máxima a 40 °C e a desnaturação ocorreu por cinética de primeira ordem. Com base nesses resultados, a energia de ativação para desnaturação (E*d) foi estimada e maiores valores foram observados para as formas PEGuiladas em comparação à enzima nativa, destacando-se a ASNase-PEG10 com aumento de 4.24 vezes (48.85 kJ.mol-1) em comparação com a forma nativa in (11.52 kJ.mol mol-1). As enzimas foram avaliadas por sua atividade residual ao longo do tempo em diferentes temperaturas de armazenamento (4 e 37 °C) e os conjugados PEGuilados mostraram-se mais estáveis após os 21 dias de ensaio. Parâmetros termodinâmicos como entalpia (ΔH‡) de desnaturação irreversível foram analisados. Valores maiores - e ), entropia (ΔS‡) de desnaturação irreversível foram analisados. Valores maiores - e ) e energia livre de Gibbs (ΔG‡) de desnaturação irreversível foram analisados. Valores maiores - e positivos - da energia livre de Gibbs foram encontrados para os conjugados PEGuilados (61.21 a 63.45 kJ.mol-1), indicando que o processo de desnaturação não ocorreu de forma espontânea. A entalpia também foi maior para os conjugados PEGuilados (18.84 a 46.08 kJ.mol-1), demonstrando o efeito protetivo da PEGuilação. Já para a entropia, os valores negativos foram mais elevados para a ASNase nativa (-0.149 J/mol.K), apontando que o processo de desnaturação aumentou a aleatoriedade e agregação do sistema, o que foi confirmado pelo dicroísmo circular. Dessa forma, a PEGuilação revelou o seu potencial de aumento de termoestabilidade para a ASNase

Ambientes de consumo alimentar e qualidade da alimentação no Reino Unido em 2014-2016 / Food consumer environments and food quality in the United Kingdom in 2014-2016

Introdução: A alimentação inadequada tem causado prejuízos imensuráveis para a população humana e para o planeta. Má-nutrição em todas as suas formas, doenças crônicas não transmissíveis, produção exacerbada de materiais de difícil decomposição, poluição de rios e oceanos são alguns dos resultados do sistema alimentar globalizado. Investigações recentes apontam o aumento no consumo de alimentos ultraprocessados em diversos países. Somado a isso, estudos indicam que os locais de consumo podem influenciar nas escolhas alimentares. Entretanto, a influência dos locais no consumo desses alimentos foi pouco explorada. Objetivo: analisar a associação entre locais de consumo e o consumo de alimentos ultraprocessados no Reino Unido entre 2014-2016. Métodos: Os dados são provenientes da National Diet and Nutrition Survey (NDNS). As informações de consumo alimentar individual, de participantes de ≥ 4 anos de idade, foram coletadas através de diário alimentar de 3 ou 4 dias consecutivos. Os locais de consumo foram categorizados em nove grupos: casa, locais institucionais, meios de transporte, cafeterias, clubes de esportes e recreação, restaurantes de serviço completo, fast food, casa de familiares e amigos e outros. Todos os itens de consumo foram classificados segundo o processamento industrial utilizando a classificação NOVA e calculou-se o percentual total e por local de participação dos itens alimentares para o total de energia (em kcal) consumida. Foi então avaliada a associação entre cada local de consumo e a participação de alimentos ultraprocessados na alimentação por meio de modelos de regressão linear brutos e ajustados para possíveis variáveis confundidoras. Esses modelos geraram coeficientes que representam o aumento da participação energética de alimentos ultraprocessados a cada aumento de ponto percentual no consumo em cada local (% kcal total). As análises foram estratificadas para crianças (4-10 anos), adolescentes (11-18 anos) e adultos (19 anos de idade ou mais). Resultados: Os alimentos ultraprocessados contribuíram com 56,3% do total de energia consumida. Entre as crianças, o consumo alimentar realizado em casa foi inversamente associado ao consumo de alimentos ultraprocessados (ß: -0,10, IC 95% -0,17, -0,03), enquanto em clubes de esportes e recreação (ß: 0,47, IC 95% 0,20, 0,73) foi diretamente associado ao consumo de alimentos ultraprocessados. Para os adolescentes, comer em casa (ß: -0,12, IC 95% -0,19, -0,05) foi inversamente associado ao consumo de alimentos ultraprocessados, assim como em restaurantes de serviço completo (ß: -0,21, IC 95% - 0,38, -0,03). O consumo em redes de fast food esteve diretamente associado ao consumo de alimentos ultraprocessados em adolescentes (ß: 0,29, IC 95% 0,12, 0,47). Finalmente, para adultos, restaurantes de serviço completo (ß: -0,13, IC 95% -0,22, -0,03) mostraram-se inversamente associados ao consumo de alimentos ultraprocessados. Já em restaurantes do tipo fast food (ß: 0,77, IC 95% 0,38, 1,17) foram diretamente associados ao consumo desses alimentos. Conclusões: os locais de consumo impactam de forma diferente no consumo de alimentos ultraprocessados, clubes de esporte e recreação e restaurantes do tipo fast food promoveram um aumento no consumo desses alimentos. Por outro lado, houve redução no consumo de alimentos ultraprocessados em casa e em restaurantes de serviço completo.

Background: Inadequate food has caused immeasurable damage to the human population and the planet. Malnutrition in all its forms, chronic non-communicable diseases, overproduction of materials that are difficult to decompose, pollution of rivers and oceans are some of the results of the globalized food system. Recent investigations point to an increase in the consumption of ultra-processed foods in several countries. In addition, studies indicate that eating locations can influence food choices. However, the influence of places on the consumption of ultra-processed foods has been little explored. Objective: to analyse the association between eating locations and consumption of ultra-processed foods in the United Kingdom between 2014-2016. Methods: data are from the National Diet and Nutrition Survey (NDNS). Individual food consumption information of participants ≥ 4 years of age was collected through a food diary for 3 or 4 consecutive days. Eating locations were categorized into nine groups: home, institutional places, on the go, coffee shops, leisure and sports places, sit-down restaurants, fast food, friends and relatives house, and other places. All consumption items were classified according to industrial processing using the NOVA classification. The total percentage and eating locations of participation of food items for the total energy (in kcal) consumed was calculated. The association between each eating location and the participation of ultra-processed foods in the diet was evaluated using crude linear regression models and adjusted for possible confounders. These models generated coefficients that represent the increase in energy participation of ultra-processed foods at each percentage point increase in consumption in each location (% kcal total). Analyses were stratified for children (4-10 years), adolescents (11-18 years), and adults (19 years or older). Results: ultra-processed foods contributed 56.3% of the total energy consumed. Among children, food consumption at home was inversely associated with consumption of ultra-processed foods (ß: -0.10, 95% CI -0.17, -0.03), while in leisure and sports places (ß: 0.47, 95% CI 0.20, 0.73) was directly associated with the consumption of ultra-processed foods. For adolescents, eating at home (ß: -0.12, 95% CI -0.19, -0.05) was inversely associated with consumption of ultra-processed foods, as well as in sit-down restaurants (ß: -0, 21, 95% CI - 0.38, -0.03). Consumption in fast food restaurants was directly associated with the consumption of ultra-processed foods in adolescents (ß: 0.29, 95% CI 0.12, 0.47). Finally, for adults, sit-down restaurants (ß: -0.13, 95% CI -0.22, -0.03) were inversely associated with the consumption of ultra-processed foods. Fast food (ß: 0.77, 95% CI 0.38, 1.17) were directly associated with the consumption of ultra-processed foods. Conclusion: eating locations have a different impact on the consumption of ultra-processed foods, leisure and sports places and fast food have promoted an increase in consumption of ultra-processed foods. On the other hand, there was a reduction in the consumption of ultra-processed foods at home and sit-down restaurants.

"A consistent stakeholder management process can guarantee the 'social license to operate'": mapping the political strategies of the food industry in Brazil / "Un proceso consistente en la gestión de las partes interesadas puede garantizar la 'licencia social para actuar'": mapeando estrategias políticas de la industria alimentaria en Brasil / "Um processo consistente de gerenciamento dos stakeholders pode garantir a 'licença social para operar'": mapeando as estratégias políticas da indústria alimentícia no Brasil

In the past, food industry actors tried to delay and weaken public health efforts to promote adequate and healthy diets in Brazil. This study aimed to identify the political strategies used by food industry actors in Brazil. We undertook a document analysis of publicly available information and interviews with eighteen key informants in public health nutrition. Data collection and analysis were carried between October 2018 and January 2019. In Brazil, food industry actors interacted with health organizations, communities, and the media. They disseminated information on nutrition and physical activity by scientific events and schools. The food industry also had allies within the government and lobbied high ranking officials. Finally, food industry actors intimidated some public health professionals, including by threats of litigation, which had the effect of silencing them. These strategies were facilitated by the use of arguments, such as the crucial role that the food industry plays in the economy and its support to the United Nations Sustainable Development Goals. Personal responsibility, moderation, and education were cited as solutions to the obesity epidemic, and there was little discussion on the broader issue of inadequate and unhealthy diets. Food industry actors in Brazil used a diverse range of political strategies, which have the potential of negatively influencing public policy, research, and practice in the country. Learning about these strategies is an essential first step, and in response, it is crucial to develop robust mechanisms to address undue influence from corporations.

En el pasado, agentes de la industria alimentaria intentaron retrasar y debilitar los esfuerzos de la salud pública para promover dietas adecuadas y saludables en Brasil. El objetivo de este estudio fue identificar las estrategias políticas usadas por los agentes de la industria alimentaria en Brasil. Realizamos un análisis documental de la información disponible públicamente y entrevistas con 18 informantes clave en nutrición dentro de la salud pública. La recolección de datos y análisis se llevaron a cabo entre octubre de 2018 y enero de 2019. En Brasil, los agentes de la industria alimentaria interactuaron con organizaciones de salud, comunidades y medios. Ellos diseminaron información sobre nutrición y actividad física mediante eventos científicos y en las escuelas. La industria alimentaria tenía también aliados dentro del gobierno y funcionarios de alto rango que hacía lobby a su favor. Finalmente, los agentes de esta industria intimidaron a algunos profesionales públicos de salud, incluso con amenazas de litigios, que tuvieron el efecto de silenciarlos. Estas estrategias se facilitaron mediante el uso de argumentos tales como el papel crucial que desempeñaba la industria alimentaria en la economía y en su apoyo para los Objetivos de Desarrollo Sostenible de la Organización de las Naciones Unidas. Se citaron la responsabilidad personal, moderación, y educación como soluciones para la epidemia de obesidad, y hubo una pequeña discusión sobre un tema tan amplio como el de las dietas inadecuadas e insanas. Los agentes de la industria alimentaria en Brasil usaron un repertorio diverso de estrategias políticas, que tienen el potencial de influenciar negativamente políticas públicas, investigaciones, así como prácticas en el país. Como primer paso es esencial aprender de estas estrategias, y en respuesta, es crucial desarrollar mecanismos robustos para abordar la influencia indebida de las corporaciones alimentarias.

No passado, os agentes da indústria alimentícia tentaram atrasar e enfraquecer os esforços de saúde pública para promoção de dietas adequadas e saudáveis no Brasil. O presente estudo tem como objetivo identificar as estratégias políticas utilizadas pelos agentes da indústria alimentícia no Brasil. Realizamos uma análise documental das informações disponíveis ao público, bem como entrevistas com 18 informantes-chave em saúde pública e nutrição. A coleta e análise de dados foi realizada entre outubro de 2018 e janeiro de 2019. No Brasil, os agentes da indústria alimentícia interagiram com organizações de saúde, comunidades e com a mídia. Difundiram informações sobre nutrição e atividade física em eventos científicos e escolas. A indústria alimentícia também apresentava aliados dentro do governo e fazia lobby junto a altos funcionários. Por fim, os agentes da indústria alimentícia intimidaram alguns profissionais da saúde pública, inclusive com ameaças de litígio, o que teve o efeito de silenciá-los. Essas estratégias foram facilitadas por argumentos como o papel crucial desempenhado pela indústria de alimentos na economia e seu apoio aos Objetivos de Desenvolvimento Sustentável da Organização das Nações Unidas. Responsabilidade pessoal, moderação e educação foram citadas como soluções para a epidemia de obesidade, e houve pouca discussão sobre a problemática mais ampla de dietas inadequadas e insalutares. Os agentes da indústria alimentícia no Brasil utilizaram uma gama diversificada de estratégias políticas com o potencial de influenciar negativamente as políticas públicas, mas também a pesquisa e a prática no país. Conhecer essas estratégias é um primeiro passo essencial e, em resposta, é crucial desenvolver mecanismos robustos para lidar com a influência indevida das corporações.

Short-chain fatty acids production by Bifidobacterium species in the presence of salep

BACKGROUND: Salep is obtained by grinding dried orchid tubers and used as a valuable ingredient in the food industry. Because of the glucomannan content of salep, it is thought to have prebiotic potential. However, there is little information in studies concerning the fermentation characteristics and potential prebiotic properties of salep. The objective of this study was to investigate the effect of salep on bifidobacterial growth by measuring the highest optical density (OD), calculating the specific growth rates, and determining the production of lactic acid and short-chain fatty acids (acetic, propionic, and butyric acid) as a result of bacterial fermentation. RESULT: The OD and pH values obtained in this study showed that salep was utilized as a source of assimilable carbon and energy by the Bifidobacterium species (BS). All Bifidobacterium strains produced lactic, acetic, propionic, and butyric acid, indicating that salep is readily fermented by these bacteria. Salep at 1% (w/v) showed a similar effect on bifidobacterial growth as that promoted by 1% (w/v) glucose used as a traditional carbon source. CONCLUSIONS: Bifidobacterium species can develop in media containing salep as well as in glucose and exhibit the potential to be used as new sources of prebiotics.

Biosurfactant produced by Candida utilis UFPEDA1009 with potential application in cookie formulation

BACKGROUND: Biosurfactants are biomolecules that have the potential to be applied in food formulations due to their low toxicity and ability to improve sensory parameters. Considering the ability of yeasts to produce biosurfactants with food-friendly properties, the aim of the present study was to apply a biosurfactant produced by Candida utilis in the formulation of cookies. RESULTS: The biosurfactant was obtained with a yield of 24.22 ± 0.23 g/L. The characterization analysis revealed that the structure of a metabolized fatty acid with high oleic acid content (68.63 ± 0.61%), and the thermogravimetric analysis demonstrated good stability at temperatures lower than 200°C, potential for food applications. The biosurfactant also exhibited satisfactory antioxidant activity at concentrations evaluated, without cytotoxic potential for cell strains, L929 and RAW 264.7, according to the (3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The incorporation of the surfactant into the dough of a standard cookie formulation to replace animal fat was carried out, achieving a softer, spongier product without significantly altering the physical and physicochemical properties or energy value. CONCLUSION: The thermal stability and antioxidant activity of the biosurfactant produced by C. utilis were verified, besides the positive contribution in the texture analysis of the cookies. Therefore, this biomolecule presents itself as a potential ingredient in flour-based sweet food formulations.

Condições higiênico-sanitárias em unidades produtoras de refeições em universidade particular / Hygienic-sanitary conditions in units of meal products at the private university

Unidades Produtoras de Refeições (UPR) são quaisquer serviços de caráter extradomiciliar na área de alimentação, para este trabalho destaca-se as cantinas em cenário universitário. A mudança de hábitos alimentares ocasionada, amplia o campo de refeições em UPRs, despertando a atenção dos consumidores perante à ingestão de um alimento seguro. O objetivo central deste trabalho foi averiguar a comercialização do alimento seguro nas UPRs em uma Universidade Particular no interior de São Paulo. Para garantir um alimento seguro, deve-se seguir a legislação, sendo uma delas a Portaria SMS-G Nº 2619/2011, aplicada especialmente as lanchonetes e similares. A legislação auxiliou no desenvolvimento da pesquisa, realizada com o consentimento de autorização dos responsáveis técnicos dos estabelecimentos em cantinas (n=5) da Universidade, utilizando seu respectivo roteiro de inspeções, denominado de Check List. Os dados foram coletados por analise visual de acordo com a realidade das UPRs. Os resultados obtidos segundo a média dos subitens de cada item e posteriormente classificados, demonstram que as condições de armazenamento, instalações e edificações, manipuladores de alimentos e documentação, são os itens que menos apresentam conformidades nas UPRs do local, e por isso enaltecidos neste artigo. Apenas a cantina 2 apresenta situação regular quanto as condições higiênico-sanitária, as demais foram classificadas como ruins. Assim, a necessidade de treinamento regular para os manipuladores é de suma importância, além da otimização constante das condições higiênico-sanitárias para garantir a comercialização de um alimento seguro.

Meal Production Units (UPR) are services of a non-household nature in the area of food, for this work to be kept as canteens in the university scenario. The change in occasional eating habits, broadens or the field of meals in UPRs, arouses the attention of consumers involved in eating a safe food. The central objective of this work, to evaluate the safe food trade in the UPRs at a Private University in the interior of São Paulo. To ensure safe food, you must follow the legislation, one of which is the Ordinance SMS-G No. 2619/2011, applied especially as snack bars and similar. The legislation assists in the development of the research, carried out with the consent of the authorization of the technicians responsible for the canteens (n = 5) of the University, using its inspection script, called Check List. The data were collected for visual analysis according to the reality of the UPRs. The results obtained after the average of sub-items of each item and classified later, demonstrating how storage conditions, facilities and buildings, food handlers and recipes, are the items that present less conformity in the local UPRs, and for these are more important in this article. Only one canteen 2 has a regular situation regarding hygienic-sanitary conditions, as others are classified as ruins. Thus, a need for regular training for handlers is of paramount importance, in addition to constant optimization of hygienic-sanitary conditions to guarantee the commercialization of a safe food.

Infância e práticas alimentares: estudo bioético sobre vulnerabilidade e risco / Childhood and eating habits: a bioethical study on vulnerability and risk / Infancia y prácticas alimentarias: estudio bioético sobre vulnerabilidad y riesgo

Resumo Trata-se de estudo de campo, qualitativo, exploratório e transversal, desenvolvido com pais de alunos de duas escolas de cidades do interior de Minas Gerais, totalizando 27 participantes. Foi realizada oficina em que os entrevistados responderam questionários e assistiram a palestra informativa. Buscou-se evidenciar os efeitos da persuasão da indústria alimentícia sobre a dieta das crianças, visto que o Brasil vem enfrentando aumento expressivo de sobrepeso, obesidade e doenças crônicas não transmissíveis também na infância. Ficou claro que a publicidade se aproveita da vulnerabilidade e falta de conhecimento de pais e responsáveis para influenciar suas escolhas. Para enfrentar esse problema, o artigo demonstra a necessidade de proteger a população por meio de educação nutricional, leis e regulamentação.

Abstract This is a qualitative, exploratory, and cross-sectional field study, developed with parents of students from two schools in cities located in the South of the state of Minas Gerais, Brazil, totaling 27 participants. The participants answered questionnaires in a workshop and attended an informational lecture. We sought to evidence the effects of the food industry on children's diets, as Brazil is experiencing a significant increase in overweight, obesity and chronic non-communicable diseases in childhood. It became clear that advertising takes advantage of the vulnerability and lack of knowledge of parents and guardians by influencing their choices. To address this problem, the article discusses the need to protect the population by nutrition education, laws, and regulations.

Resumen Este es un estudio de campo, cualitativo, exploratorio y transversal, desarrollado con padres de alumnos de dos escuelas de ciudades del interior de Minas Gerais, con un total de 27 participantes. Se realizó un taller en el que los entrevistados respondieron cuestionarios y asistieron a una charla informativa. Se pretendió poner de manifiesto los efectos de la persuasión de la industria alimenticia en la dieta de los niños, dado que Brasil enfrenta un aumento significativo del sobrepeso, de la obesidad y de las enfermedades crónicas no transmisibles, incluso en la infancia. Se evidenció que la publicidad se aprovecha de la vulnerabilidad y de la falta de conocimiento de padres y responsables para influenciar sus elecciones. Para afrontar ese problema, el artículo demuestra la necesidad de proteger a la población por medio de educación nutricional, de legislación y de regulación.

Código Nutricia: nutrición y conflicto de interés en la academia / Nutricia Code: nutrition and conflict of interest in academia

Resumen: Para combatir la mala nutrición en todas sus formas es necesario integrar acciones desde la sociedad en su conjunto. El interés superior de los actores involucrados en generar estas acciones debe ser la salud. No obstante, existen actores con conflictos de interés que interfieren en el diseño, planeación, implementación y monitoreo de las políticas derivadas de dichas acciones. A fin de mitigar el efecto adverso que generan estos conflictos desde la etapa de generación de evidencia hasta el diseño e implementación de las políticas, se propone este código. Se invita a la comunidad de nutrición y salud a adoptarlo, promoverlo y sumarse al mismo, para favorecer el avance de las acciones y políticas necesarias sin interferencia de la industria y resolver el problema de la mala nutrición.

Abstract: To combat malnutrition in all its forms, actions must be integrated from society as a whole. The main interest of the actors involved in these actions should be health. However, there are actors with conflict of interest that interfere in the design, planning, implementation and monitoring of public health nutrition policies. In order to mitigate the adverse effect that result from these conflicts, from evidence generation to the design and implementation of policies, this Code is proposed. It invites the nutrition and health community to adopt it, promote it and subscribe it, and to favor the advancement of actions and policies without industry interference to address the problem of malnutrition.

Bisfenol-A: cuantificacion en orina de mujeres embarazadas por cromatografia gaseosa-espectrometria de masa / Bisfenol-A: quantification in urine of pregnant women by gas chromatography-mass spectrometry / Bisfenol-A: quantificacao em urina de gestantes por cromatografia gasosa-espectrometria de massa

El uso de bisfenol-A (BPA) a nivel de la industria global se ha venido incrementando en los ultimos anos, y fueron los mercados emergentes los impulsores de esta demanda creciente. Las aplicaciones de BPA en la industria de los alimentos y bebidas representan solo del 3 al 4% del consumo global de policarbonato, pero su uso esta siendo reexaminado debido a que se conocieron varios trabajos cientificos que indican la existencia de una relacion directa entre el BPA y los efectos adversos para la salud. La contaminacion de los alimentos y bebidas se produce por migracion del BPA desde los envases que los contienen (alimentos enlatados, vinos, etc.), y es la principal fuente de exposicion en el humano. Para evaluar dicha exposicion se desarrollo y valido un metodo analitico por cromatografia gaseosa acoplada a espectrometria de masa para la cuantificacion de BPA total en orina de mujeres embarazadas atendidas en el Hospital Italiano de Buenos Aires en el ano 2013, con un limite de cuantificacion de 2,0 ng/mL y un limite de deteccion de 0,8 ng/mL. De las 149 muestras de orina analizadas, el 66,4% fueron cuantificables, con la mediana de BPA total de 4,8 ng/mL (4,3 ng/mg de creatinina) y la media geometrica de 4,8 ng/mL (4,7 ng/mg de creatinina).

The use of bisphenol-A (BPA) at the level of the global industry has been increasing in recent years, with emerging markets being the drivers of this growing demand. BPA applications in the food and beverage industry represent only 3 to 4% of the global consumption of polycarbonate, but its use is being reexamined because several scientific works were reported indicating the existence of a direct relationship between BPA and adverse effects on health. The contamination of food and beverages is produced by the migration of BPA from the containers that hold them (canned foods, wines, etc.) and it is the main source of exposure in humans. To evaluate this exposure, an analytical method was developed by gas chromatography coupled to mass spectrometry for the quantification of total BPA in urine of pregnant women treated at the Hospital Italiano de Buenos Aires in 2013, with a limit of quantification of 2.0 ng/mL and of detection of 0.8 ng/mL. Of the 149 urine samples analyzed, 66.4% were quantifiable, with a median total BPA of 4.8 ng/mL (4.3 ng/mg creatinine) and a geometric mean of 4.8 ng/mL (4.7 ng/mg creatinine).

O uso de bisfenol-A (BPA) ao nivel da industria global foi aumentando nos ultimos anos, e foram os mercados emergentes que deram impulso a essa demanda crescente. As aplicacoes de BPA na industria de alimentos e bebidas representam apenas 3 a 4% do consumo global de policarbonato, mas seu uso esta sendo reexaminado visto que varios trabalhos cientificos indicando a existencia de uma relacao direta entre o BPA e os efeitos adversos na saude foram conhecidos. A contaminacao dos alimentos e bebidas e produzida pela migracao de BPA das embalagens que os contem (alimentos enlatados, vinhos, etc.) e e a principal fonte de exposicao em humanos. Para avaliar esta exposicao, foi desenvolvido e avaliado um metodo analitico por cromatografia gasosa acoplada a espectrometria de massas para a quantificacao do BPA total na urina de gestantes atendidas no Hospital Italiano de Buenos Aires em 2013, com um limite de quantificacao de 2,0 ng/mL e um limite de deteccao de 0,8 ng/mL. Das 149 amostras de urina analisadas, 66,4% foram quantificaveis, com uma mediana de BPA total de 4,8 ng/mL (4,3 ng/mg de creatinina) e a media geometrica de 4,8 ng/mL (4,7 ng/mg de creatinina).

Binômio tempo e temperatura e qualidade microbiológica de macarrão espaguete produzido e distribuído por unidade de alimentação e nutrição em Fortaleza-CE / Time and temperature binomial and microbiological quality of spaghetti pasta produced and distributed by food and nutrition unit in Fortaleza-CE

O trabalho teve como objetivo avaliar o binômio tempo x temperatura e a qualidade microbiológica de macarrão tipo espaguete refogado produzido e distribuído por uma empresa de alimentação e nutrição. Pesquisa transversal realizada no mês de março de 2019, em uma UAN de grande porte na cidade de Fortaleza, CE, nos dias em que o macarrão tipo espaguete refogado se fez presente no cardápio do jantar de uma universidade pública. Aferiu-se a temperatura do macarrão durante os processos de cocção, transporte, recebimento e em três momentos da durante a distribuição. Amostras do macarrão servido foram coletadas e encaminhadas para análise microbiológica. As temperaturas atingiram as médias de 94,2ºC na cocção, 40,7ºC no transporte, 39ºC no recebimento e 39ºC, 41,3ºC e 43,7ºC nos três momentos da distribuição. As análises microbiológicas das amostras evidenciaram que o macarrão estava dentro dos parâmetros considerados adequados de acordo com a RDC nº 12 e, posteriormente, à IN nº 60 da ANVISA, legislação vigente para os microrganismos avaliados...

The objective of this study was to evaluate the time x temperature binomial and the microbiological quality of braised spaghetti noodles produced and distributed by a food and nutrition company. Cross-sectional study conducted in March in a large FNU in the city of Fortaleza, CE, in the days which the braised spaghetti pasta was present on the dinner menu of a public university. The spaghetti temperatures were measured during the cooking, transport, receivement and three times during distribution processes. Samples of the served pasta were collected and sent for microbiological analysis. Temperatures reached the averages of 94.2ºC in cooking, 40.7ºC in transportation, 39ºC in receivement and 39ºC, 41.3ºC and 43.7ºC in the three moments of distribution. The microbiological analyzes of the samples showed that the noodles were within the parameters considered adequate according to the RDC no 12 and, subsequently, to ANVISA IN no 60, current legislation for the evaluated microorganisms

A Thermotolerant Xylan-Degrading Enzyme Is Produced by Streptomyces malaysiensis AMT-3 Using by-Products From the Food Industry

Abstract This study evaluated the production of endoxylanases by Streptomyces malaysiensis AMT-3 in submerged fermentation using by-products of the food industry at 28ºC. In shake-flasks experiments, the highest endoxylanase activity of 45.8 U.mL-1 was observed within 6 days in a medium containing (w/v) 2.5% wheat bran and 1.2% corn steep liquor. The same culture conditions were used to evaluate the enzyme production in a 2 L stirred tank reactor under different agitation (300, 450 and 600 rev.min-1) and aeration (30 and 60 L.h-1) conditions. The use of 450 rev.min-1 coupled to an aeration of 90 L.h-1 resulted on 81.3 U.mL-1 endoxylanase activity within 5 days. The effect of temperature and pH on endoxylanase activity and stability showed the highest activity at 60 ºC and pH 6.0. Zymography showed the presence of three xylanolytic bands with molecular masses of 690, 180 and 142 kDa. The results showed that the thermotolerant actinobacterial endoxylanase can be produced in high titers using by-product of the food industry.

Involvement of the food industry in nutrition conferences in Latin America and the Caribbean

Objective: Identify and characterise the food industry's involvement in nutrition and dietetics national and regional events in Latin America and the Caribbean. Design: Between February and April 2020, we conducted desk-based searches for nutrition and dietetics events held in the region between January 2018 and December 2019. Online freely accessible, publicly available information was collected on the involvement of the food industry through: sponsorship of events; sponsorship of sessions; speakers from the food industry; scholarships, fellowship, grants, awards and other prizes and; exhibition space/booths. Setting: Nutrition and dietetics events in Latin America and the Caribbean. Results: Thirty-one events held in twenty countries of the region had information publicly available online at the period of data collection. There was a lack of transparency on the involvement of industry actors in these events. When information was publicly available, we found that a total of ninety-two food industry actors sponsored 88 % of these events. Conclusions: There is a mostly unreported, but likely extensive, involvement of food industry actors in nutrition and dietetics events in Latin America and the Caribbean.(AU)