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1.
Braz. j. biol ; 82: e256409, 2022. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1350298

ABSTRACT

Abstract In this research, some plant seeds powder was evaluated to find their potential effect to rule diseases of food poisoning. Antimicrobial effect of five plant seeds was examined contra Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella. pneumonia and Candida albicans by using well diffusion method. Antimicrobial activity studies revealed high potential activity of plant seeds powder of Nigella sativa L., cucurbita pepo, Sesamum radiatum, Trigonella foenum-graecum, Linum usitatissimum with variable efficiency contra tested microbial strains with concentration of 100 mg/ml, except Sesamum radiatum scored no effect. The T. foenum and N. sativa seed powder showed the largest inhibition zone (24-20 mm) contra K. pneumonia, followed by S. aureus (20-18 mm) and C. albicans (15mm) respectively. The five plant seeds powder exhibited bacteriostatic and bactericidal effects with MIC's 20 and MBC 40 mg/ml against K. pneumonia, and MIC's 40 and MBC 60 mg/ml against S. aureus. The results of this study indicated that plants seeds powder have promising antimicrobial activities and their potential applications in food process. It could be utilized as a natural medicinal alternative instead of chemical substance.


Resumo Nesta pesquisa, o pó de sementes de plantas foi avaliado para encontrar seu efeito potencial no controle de doenças de intoxicação alimentar. O efeito antimicrobiano de cinco sementes de plantas foi examinado contra Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia e Candida albicans usando o método de difusão bem. Estudos de atividade antimicrobiana revelaram alto potencial de atividade de sementes de plantas em pó de Nigella sativa L., Cucurbita pepo, Sesamum radiatum, Trigonella foenum-graecum, Linum usitatissimum com eficiência variável contra cepas microbianas testadas com concentração de 100 mg / ml, exceto Sesamum radiatum com pontuação não efeito. O pó de sementes de T. foenum e N. sativa apresentou a maior zona de inibição (24-20 mm) contra K. pneumonia, seguido por S. aureus (20-18 mm) e C. albicans (15 mm), respectivamente. O pó de cinco sementes de plantas exibiu efeitos bacteriostáticos e bactericidas com MIC's 20 e MBC 40 mg / ml contra K. pneumonia, enquanto MIC's 40 e MBC 60 mg / ml contra S. aureus. Os resultados deste estudo indicaram que os pós de sementes de plantas apresentam promissoras atividades antimicrobianas e suas potenciais aplicações em processos alimentícios. Ele poderia ser utilizado como alternativa medicinal natural em vez de substância química.


Subject(s)
Staphylococcus aureus , Foodborne Diseases , Seeds , Plant Extracts/pharmacology , Microbial Sensitivity Tests , Anti-Bacterial Agents/pharmacology
2.
Braz. j. biol ; 81(4): 940-953, Oct.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153447

ABSTRACT

Abstract Nowadays food borne illness is most common in people due to their epidemic nature. These diseases affect the human digestive system through bacteria, viruses and parasites. The agents of illness are transmitted in our body through various types of food items, water and uncooked. Pathogens show drastic changes in immunosuppressant people. This review gives general insights to harmful microbial life. Pakistan is a developed country and because of its improper food management, a lot of gastrointestinal problems are noted in many patients. Bacteria are most common agents to spread diarrhoea, villi infection, constipation and dysenteric disease in human and induce the rejection of organ transplant. Enhancement of their lifestyle, properly cooked food should be used and to overcome the outbreak of the diseases.


Resumo Hoje em dia, as doenças transmitidas por alimentos são mais comuns em pessoas devido à sua natureza epidêmica. Essas doenças afetam o sistema digestivo humano por meio de bactérias, vírus e parasitas. Os agentes das doenças são transmitidos em nosso corpo por meio de diversos tipos de alimentos, água e crus. Os patógenos mostram mudanças drásticas em pessoas imunossupressoras. Esta revisão fornece uma visão geral da vida microbiana prejudicial. O Paquistão é um país desenvolvido e, devido ao seu manejo alimentar inadequado, muitos problemas gastrointestinais são observados em muitos pacientes. As bactérias são os agentes mais comuns para espalhar diarreia, infecção de vilosidades, obstipação e doença disentérica em humanos e induzem a rejeição de transplantes de órgãos. Melhoria de seu estilo de vida, alimentos devidamente cozidos devem ser utilizados e para superar o aparecimento de doenças.


Subject(s)
Humans , Foodborne Diseases/epidemiology , Pakistan , Bacteria , Diarrhea
3.
Biomédica (Bogotá) ; 41(supl.2): 165-179, oct. 2021. tab, graf
Article in Spanish | LILACS | ID: biblio-1355768

ABSTRACT

Resumen | Introducción. Listeria monocytogenes es un patógeno transmitido por alimentos que causa infecciones en humanos, entre ellas, meningitis, meningoencefalitis y septicemias, así como abortos. Con la tipificación serológica se han identificado 13 serotipos, siendo el 4b el causante de la mayoría de los brotes en el mundo. Objetivo. Determinar la frecuencia y la distribución de los serotipos y subtipos moleculares de L. monocytogenes aislados de alimentos en Colombia entre el 2010 y el 2018. Materiales y métodos. Se hizo un estudio descriptivo y retrospectivo a partir del análisis de 2.420 aislamientos que fueron identificados como L. monocytogenes y otras especies, por medio de pruebas bioquímicas, serológicas y de subtipificación molecular mediante electroforesis en gel de campo pulsado (PFGE). Resultados. De los 2.420 aislamientos recibidos, 2.326 fueron confirmados como L. monocytogenes. Los serotipos encontrados fueron: 4b (52%), 4d-4e (14,5%), 1/2a (11%), 1/2c (9,4%), 1/2b (9 %), y 3a, 3b, 3c, 4c, 4d, 4e y 7 (menos de 2%). Procedían de Bogotá (43%), Antioquia (25%), Valle (10%), Nariño (9%) y otros departamentos (7%). La caracterización genotípica agrupó los aislamientos evaluados en 167 patrones de PFGE; los perfiles más frecuentes se presentaron en productos lácteos, cárnicos y alimentos preparados. Conclusión. El 96,1 % de los aislamientos correspondieron a L. monocytogenes, con una buena concordancia entre el aislamiento y la identificación; el serotipo 4b, extremadamente virulento, fue el más frecuente. El análisis molecular evidenció la posible diseminación y permanencia en el tiempo de varios serotipos, lo que resalta la importancia de incluir este patógeno en los programas de vigilancia epidemiológica en alimentos.


Abstract | Introduction: Listeria monocytogenes is a food-borne pathogen that may cause infections in humans such as meningitis, meningoencephalitis, and septicemia, as well as abortions. By serological typing 13 serotypes have been identified of which 4b is responsible for most of the outbreaks in the world. Objective: To determine the frequency and distribution of serotypes and molecular subtypes of L. monocytogenes isolated in Colombia from food from 2010 to 2018. Materials and methods: We conducted a retrospective and descriptive study based on the analysis of 2,420 isolates confirmed as L. monocytogenes and other species using biochemical and serological tests, and pulsed-field gel electrophoresis (PFGE) for molecular subtyping. Results: Of the 2,420 isolates received, 2,326 were confirmed as L. monocytogenes. The serotypes found were 4b (52%), 4d-4e (14.5%), 1/2a (11%), 1/2c (9.4%), 1/2b (9%), and 3a, 3b, 3c, 4c, 4d, 4e and 7 (less than 2%). The isolates came from Bogotá (43%), Antioquia (25%), Valle (10%), Nariño (9%), and other departments (7%). The genotypic characterization grouped the isolates in 167 PFGE patterns. The most frequent patterns were identified in various dairy and meat products, and in prepared foods. Conclusion: A 96.1% of the isolates corresponded to L. monocytogenes showing good agreement between isolates and identification. Serotype 4b, highly virulent, was the most frequent. The molecular analysis showed the possible dissemination and permanence over time of several serotypes, which highlights the importance of including this pathogen in epidemiological food surveillance programs.


Subject(s)
Foodborne Diseases , Listeria monocytogenes , Electrophoresis, Gel, Pulsed-Field
4.
Rev. bras. anal. clin ; 53(1): 80-84, 20210330. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1291760

ABSTRACT

Objetivo: O presente estudo objetivou pesquisar enteroparasitas em morangos frescos comercializados in natura no município de Goiânia, Goiás. Métodos: Foram analisadas 51 amostras de morangos, sendo 25 oriundas de supermercados e 26 de rua/comércio livre, entre agosto/2019 e dezembro/2019, em diversos bairros das regiões norte, noroeste, sul e central do município de Goiânia, Goiás, empregando-se as técnicas de Faust, Hoffman, Ritchie e Coloração de Kinyoun (Ziehl-Neelsen modificado). Resultados: Das 51 amostras analisadas, 78,4% foram positivas para enteroparasitas. Das quarenta amostras positivas, em 25 (62,5%) foram identificados apenas cistos de protozoários, em oito (20,0%) apenas ovos de nematódeos e em sete (17,5%) cistos de protozoários e ovos de nematódeos, simultaneamente. As espécies encontradas foram: Ascaris lumbricoides, Entamoeba coli, Entamoeba histolytica, Endolimax nana e Giardia lamblia. Todos os enteroparasitas foram identificados pelas técnicas de Hoffman e Faust. Conclusão: O estudo evidenciou elevada contaminação dos morangos comercializados em Goiânia, Goiás por enteroparasitas, com destaque para os protozoários. O consumo de morangos frescos in natura pode oferecer risco à saúde de seus consumidores, sendo crucial orientar a população sobre a correta higienização deste alimento antes de seu consumo a fim de evitar a ocorrência de enteroparasitoses.


Objective: This study aimed to research enteroparasites in fresh in natura strawberries marketed in the city of Goiânia, Goiás. Methods: A total of 51 strawberry samples were analyzed. These samples, 25 were from supermarkets and 26 from street/free trade, between August/2019 and December/2019, in several neighborhoods in the north, northwest, south and central regions of the municipality of Goiânia, Goiás, using the techniques of Faust, Hoffman, Ritchie and Kinyoun Coloring (modified Ziehl-Neelsen). Results: Of the 51 samples analyzed, 78.4% were positive for enteroparasites. Of the 40 positive samples, in 25 (62.5%) just protozoan cysts were identified, in eight (20.0%) just nematode eggs and in seven (17.5%) protozoan cysts and nematode eggs, simultaneously. The species found were: Ascaris lumbricoides, Entamoeba coli, Entamoeba histolytica, Endolimax nana, and Giardia lamblia. The enteroparasites founded were identified by the techniques of Hoffman and Faust.Conclusion: The study showed high contamination of strawberries marketed in Goiânia, Goiás by enteroparasites, with emphasis on protozoa. The consumption of fresh strawberries, in natura, can pose a risk to the health of its consumers, being crucial to guide the population on the correct hygiene of this food before its consumption to avoid the occurrence of enteroparasitosis.


Subject(s)
Parasitology , Food Analysis , Foodborne Diseases
5.
Biomédica (Bogotá) ; 41(1): 41-51, ene.-mar. 2021. tab, graf
Article in Spanish | LILACS | ID: biblio-1249057

ABSTRACT

Resumen | Introducción. Salmonella entérica subsp. entérica serovar Give se encuentra en mamíferos rumiantes, cerdos, aves y ambientes acuáticos, pero rara vez en humanos. En Colombia este serotipo ocupó el decimoprimer lugar en frecuencia en la vigilancia por laboratorio de la enfermedad diarreica aguda entre el 2000 y el 2013. Objetivo. Caracterizar el fenotipo y el genotipo de S. Give en aislamientos relacionados con un brote de enfermedad transmitida por alimentos en el departamento de Vichada en la quinta semana epidemiológica del 2015. Materiales y métodos. Se buscó Salmonella spp. en 37 muestras de materia fecal con el método de estudio del Instituto Nacional de Salud. La muestra de sardinas enlatadas fue procesada según la norma ISO6579:2002 Cor.1:2004. Se determinó el serotipo en los aislamientos confirmados mediante serología o PCR en tiempo real, y se hicieron pruebas de sensibilidad a antimicrobianos y electroforesis en gel de campo pulsado con las enzimas Xbal y BlnI. Resultados. Todos los aislamientos de origen humano (11) y el aislamiento del alimento (1), se identificaron como S. Give y este último presentó resistencia a la tetraciclina. El análisis por PFGE-XbaI agrupó bajo el patrón COIN15JEXX01.0005 diez aislamientos de origen humano y a los restantes bajo el COIN15JEXX01.0006, con un 96,3 % de similitud. Los resultados de todos los aislamientos se confirmaron con la enzima BlnI; cuatro de ellos (tres humanos y el del alimento) se agruparon bajo el patrón COIN15JEXA26.002, con un porcentaje de similitud del 95,65 %. Conclusión. El estudio confirmó que las sardinas enlatadas se relacionaron con la transmisión de S. Give en el brote, que es el tercero ocasionado por este serotipo en Colombia.


Abstract | Introduction: Salmonella enterica subsp. enterica serovar Give is found in ruminants, pigs, poultry, and aquatic environments, but rarely in humans. In Colombia, this serotype was ranked 11th. in the laboratory surveillance of acute diarrheal disease between 2000 and 2013. Objective: To characterize phenotypic and genotypic isolates of Salmonella related to an outbreak of foodborne Illness in the department Vichada in the fifth epidemiological week of 2015. Materials and methods: Following the Instituto Nacional de Salud method, we tested 37 fecal samples for Salmonella spp. while the sample of canned sardines was processed according to the ISO 6579:2002 Cor.1:2004 standard. The isolates were confirmed by serology and/or real-time PCR, antimicrobial susceptibility tests, and pulsed-field gel electrophoresis with the XbaI and BlnI enzymes. Results: All human isolates (11) and that from food (1) were identified as S. Give. The food isolate exhibited tetracycline resistance. PFGE analysis with XbaI grouped ten isolates from samples of human origin in pattern COIN15JEXX01.0005 and the remaining isolates in COIN15JEXX01.0006 with 96.3% similarity. All isolates were confirmed with the BlnI enzyme, and four (three human isolates and the one from food) were matched to the pattern COIN15JEXA26.002 with 95.65% similarity. Conclusion: Our study confirmed that canned sardines were related to the transmission of S. Give in the outbreak, which is the third one caused by this serotype in Colombia.


Subject(s)
Salmonella , Foodborne Diseases , Disease Outbreaks , Colombia , Epidemiological Monitoring
6.
Acta amaz ; 51(1): 79-84, jan.-mar. 2021.
Article in English | LILACS | ID: biblio-1353172

ABSTRACT

A doença de Chagas, enfermidade causada pelo protozoário Trypanosoma cruzi, tem sido relacionada com frequência à transmissão oral pelo consumo de açaí. Métodos moleculares que fornecem uma identificação rápida e precisa do patógeno para a detecção da presença do parasita são de extrema importância para a detecção da presença do parasita neste alimento. Este estudo teve como objetivo otimizar a detecção de DNA de T. cruzi em polpa de açaí por meio da reação em cadeia da polimerase (PCR). Foram preparadas várias diluições das formas tripomastigotas de T. cruzi DTU TcI cultivadas em meio de cultivo Liver Infusion Tryptose. O DNA de T. cruzi foi extraído das células e submetido à PCR. Posteriormente, as diluições da cultura foram adicionadas às polpas de açaí para avaliar o limite de detecção do novo ensaio de PCR otimizado. Mostramos que nosso ensaio pode detectar DNA de T. cruzi em polpas de açaí na concentração de 1.08 × 10-10 ng µL-1. Concluímos que a metodologia desenvolvida se mostra eficaz e pode ser uma ferramenta importante para a detecção de contaminação por T. cruzi em açaí.(AU)


Subject(s)
Chagas Disease , Foodborne Diseases , Genetic Carrier Screening , Noxae
7.
Rev. argent. salud publica ; 13: 1-7, 5/02/2021.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1342308

ABSTRACT

INTRODUCCIÓN: La manipulación de alimentos dentro del ámbito hospitalario juega un papel crucial en la cadena causal de enfermedades transmitidas por alimentos. Actualmente no existe información sobre conocimientos, prácticas y valoraciones de los manipuladores de alimentos a nivel local. El objetivo de este estudio fue evaluar a los manipuladores de alimentos de hospitales públicos de la provincia de Buenos Aires y su asociación con características sociodemográficas, laborales y de capacitación. MÉTODOS: Se realizó un estudio transversal analítico. Se envió una encuesta virtual a los hospitales públicos de la provincia para que fuera distribuida a todos los manipuladores de alimentos. Se relevaron datos sociodemográficos, laborales y de capacitación. Se evaluaron conocimientos y prácticas mediante preguntas de opción múltiple y se indagaron valoraciones personales sobre su trabajo. RESULTADOS: La encuesta fue completada por 561 manipuladores de 56 hospitales. Más del 80% había recibido algún tipo de capacitación. El 22,9% presentó conocimientos suficientes y el 15,3%, prácticas adecuadas. La valoración de prácticas adecuadas se asoció a mayor antigüedad, servicios tercerizados y a la realización de 5 o más capacitaciones en servicio. La escasez de elementos de trabajo y la infraestructura inadecuada fueron las principales barreras. DISCUSIÓN: La realización de capacitaciones en servicio y la presencia de personal con mayor antigüedad podrían mejorar la manipulación de alimento.


Subject(s)
Food Safety , Foodborne Diseases , Food Services
8.
Arq. Inst. Biol ; 88: e00212020, 2021.
Article in English | LILACS, VETINDEX | ID: biblio-1349023

ABSTRACT

The consumption of meat and meat products can pose consumers into risk due to the presence of biological hazards that can cause foodborne diseases. Thus, this study aimed to compare the microbiological quality of illegal and inspected salami sold in the state of São Paulo, Brazil. For this purpose, 80 salami samples (40 illegal and 40 inspected) were purchased and their microbiological quality was assessed according to the protocol established by the Brazilian Ministry of Health. All samples were considered as acceptable for consumption according to the Brazilian law. However, the samples of illegal salami were significantly higher contaminated with bacteria belonging to the genus Staphylococcus (p = 0.002) and had a higher trend to be contaminated with total coliforms (p = 0.08) and thermotolerant ones (p = 0.07) compared to inspected salami. Salmonella spp. and coagulase-positive Staphylococcus were not detected. In conclusion, although all samples were considered as safe for consumption, illegal salami had a worse microbiological quality when compared to inspected ones.


Subject(s)
Humans , Animals , Staphylococcus/pathogenicity , Meat/microbiology , Meat Products , Salmonella , Bacteria , Health Surveillance , Food Quality , Public Health , Commerce , Food Safety , Foodborne Diseases
9.
Arq. Inst. Biol ; 88: e0702019, 2021. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1348957

ABSTRACT

Products such as milk and cheese produced by hand and sold by small producers in open markets and at home are a reality in Brazil, despite legal prohibitions. In many cases, this leads to the production of food without hygienic conditions, which may constitute an important source of transmission of foodborne diseases and a danger to public health. This study proposes to examine the hygienic-sanitary quality of milk and cheese sold illegally in municipalities of northern Mato Grosso, Brazil, to undertake a phenotypical investigation of the presence of resistance of isolated colonies to antimicrobials and to detect the production of ß-lactamase enzymes: extended-spectrum ß-lactamase (ESBL), AmpC ß-lactamases (AmpC) and carbapenemases. The 25 milk and 37 cheese samples analyzed were subjected to the most probable number (MPN) test, isolation on eosin-methylene blue agar (EMB) agar and Escherichia coli identification by biochemical tests and disk diffusion test. Results showed that 76% of the milk samples and 67.57% of the cheese samples had thermotolerant coliform counts above the value allowed by the legislation. The milk and cheese isolates showed 15.79 and 5.88% resistance, respectively, to at least one of the tested antimicrobials. No ß-lactamase enzyme production was observed in the isolates.


Subject(s)
Cheese , Milk , Escherichia coli , Health Surveillance of Products , Food Contamination , Food Hygiene , Food Inspection , Public Health , Foodborne Diseases
10.
Acta sci. vet. (Impr.) ; 49: Pub. 1835, 2021. mapa, tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1363707

ABSTRACT

The artisanal goat coalho cheese is one of the products obtained that stand out in the dairy goat farming of the Northeast of Brazil. Despite its importance, goat cheese is often made under inadequate hygienic-sanitary conditions and usually uses raw goat's milk, increasing the risk of product contamination. Among the pathogens carried by goat coalho cheese, Staphylococcus aureus stands out, being responsible for cases of food poisoning and persistent infections that are difficult to treat. This study aimed to evaluate the contamination, genotypic and phenotypic resistance of Staphylococcus aureus isolated from artisanal coalho cheese made with goat milk produced in the Northeast region of Brazil. This study analyzed only artisanal coalho cheeses made with raw goat's milk and purchased directly from farms. Twelve samples of artisanal coalho cheeses made with raw goat's milk were collected (1 sample per property) in 8 municipalities in the state of Pernambuco, Northeast region of Brazil. For microbiological analysis of enumeration of Colony Forming Units (CFU/g) of Staphylococcus spp. the methodology recommended by the International Organization for Standardization (2019) and recognized by the Ministry of Agriculture, Livestock and Supply was used. After enumeration, 5 colonies were selected per enumerated plate, a total of 180 Staphylococcus spp. was obtained. These were subjected to thermal extraction of genetic material to search for the nuc gene by Polymerase Chain Reaction, the isolates carrying the nuc gene were subjected to genotypic and phenotypic evaluation of antimicrobial resistance. After the phenotypic analysis, the Multiple Antimicrobial Resistance Index was evaluated. In all samples, Staphylococcus spp. and were considered unfit for consumption, with the lowest count being 9.4x103 CFU/g and the highest 6.4x106 CFU /g. Of the 180 isolates, 28.34% (51/180) were positive for the detection of the nuc gene. All resistance genes except mecA, mecC, and norB were detected. Of the 51 S. aureus isolates, 31.37% (16/51) were considered multi-resistant and presented a Multiple Antimicrobial Resistance Index above 0.2. After microbiological analysis it was found that all samples of coalho cheese were out of standards and unfit for human consumption in accordance with Ordinance no 146/1996 of the Ministry of Agriculture, Livestock and Supply of Brazil. Furthermore, the contamination of goat coalho cheeses is a risk to public health. During sample collection were found inadequate hygiene conditions in the environment used for cheese production. The presence of Staphylococcus aureus can be attributed to hygienic-sanitary failures in cheese production. From a health point of view, it is even more alarming when it comes to S. aureus carrying resistance genes. Although the 51 S. aureus isolates did not carry the mecA, mecC, norB genes and did not show phenotypic resistance to cefoxitin and oxacillin, all other genes were detected, indicating the circulation of S. aureus carrying the tet(L) genes, tet(M), tet-38, msrA, norA, and norC, which so far had not been reported in the production chain of goat coalho cheese in Brazil. Furthermore, the evaluation of the Multiple Antimicrobial Resistance Index identified the occurrence of multiple resistance to antimicrobials in 31.37% (16/51) of S. aureus at high risk to human health. The results obtained are quite worrying and serve as a warning to the scientific community and the Food Safety and Hygiene Inspection Services.(AU)


Subject(s)
Staphylococcus aureus/isolation & purification , Cheese/microbiology , Identity and Quality Standard for Products and Services , Food Safety , Foodborne Diseases/microbiology
11.
Brasília; Brasil. Ministério da Saúde; 2021.
Non-conventional in Portuguese | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1373489

ABSTRACT

Este Manual é um instrumento que subsidia o treinamento em vigilância epidemiológica das doenças de transmissão hídrica e alimentar (VE-DTHA), direcionado para profissionais de saúde que atuam na investigação de surtos ou de casos de doenças de notificação compulsória relacionadas à transmissão hídrica e alimentar.


Subject(s)
Humans , Disease Outbreaks/prevention & control , Waterborne Diseases/epidemiology , Public Health Surveillance/methods , Foodborne Diseases/epidemiology , Brazil/epidemiology , Waterborne Diseases/prevention & control , Foodborne Diseases/prevention & control
12.
Biomédica (Bogotá) ; 40(4): 594-598, oct.-dic. 2020. graf
Article in Spanish | LILACS | ID: biblio-1142424

ABSTRACT

Resumen: La intoxicación escombroide es ocasionada por el consumo de ciertos tipos de pescado (de la familia Scombridae), comúnmente el atún, los cuales acumulan grandes concentraciones de histamina cuando los procedimientos de refrigeración son inadecuados, ocasionando en quienes los consumen síntomas muy similares a los de una alergia alimentaria, por lo que es frecuente que no se diagnostique correctamente. Generalmente, los síntomas desaparecen en pocas horas y no suelen ser graves, excepto algunos casos descritos en la literatura especializada, de hipotensión, broncoespasmo, dificultad respiratoria, taquicardia supraventricular e, incluso, infarto agudo de miocardio. Este fue, precisamente, el caso de una mujer que ingresó al servicio de urgencias de un hospital de tercer nivel de Medellín a los pocos minutos de haber ingerido atún con una sintomatología típica de la intoxicación, pero con taquicardia supraventricular, una de sus manifestaciones graves y atípicas.


Abstract: Scombroid poisoning is caused by the consumption of certain types of fish (from the Scombridae family), especially tuna. Due to inadequate refrigeration procedures, these fish have high levels of histamine which generate symptoms similar to those of a food allergy in their consumers, so it is frequently underdiagnosed. It is self-limited in a few hours and the symptoms are usually not serious, except for specific cases reported in the literature of hypotension, bronchospasm, respiratory distress, tachyarrhythmias, and even acute myocardial infarction. We report here the case of a woman admitted to the emergency department of a third level hospital in Medellín a few minutes after eating tuna with the typical symptoms of intoxication, as well as tachyarrhythmias, a serious and atypical manifestation.


Subject(s)
Tuna , Foodborne Diseases , Arrhythmias, Cardiac , Histamine
13.
Arq. bras. med. vet. zootec. (Online) ; 72(2): 615-622, Mar./Apr. 2020. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1128492

ABSTRACT

A fabricação de queijo coalho artesanal elaborado com leite de cabra é composta pelas etapas de obtenção do leite, refrigeração, manipulação e armazenamento, que aumentam o risco de contaminação do produto. Objetivou-se neste estudo avaliar o nível de contaminação por Staphylococcus aureus em amostras de queijo coalho artesanal produzido com leite de cabra cru no estado de Pernambuco, Brasil, bem como avaliar a concordância entre a técnica oficial da Instrução Normativa nº62/2003 (Mapa) e a técnica molecular (gene nuc) para identificar S. aureus no queijo. Houve crescimento de colônias típicas de Staphylococcus aureus em 100% das amostras, e a contagem variou de 7,0×103 a 8,6×106 UFC/g. Das 30 amostras analisadas, 18 (60,0%) apresentaram valores superiores ou iguais a 105UFC/g, e 21 (70,0%) estavam contaminadas por S. aureus. A concordância entre os métodos de diagnóstico de S. aureus em queijo coalho caprino foi moderada. O nível de contaminação dos queijos revela a necessidade de ações de melhoria das condições de elaboração do produto, a fim de garantir um produto seguro aos consumidores.(AU)


The manufacture of artisanal Coalho cheese made from goat's milk is composed of the steps of obtaining milk, refrigeration, handling and storage that increase the risk of product contamination. The objective of this study was to evaluate the level of contamination by Staphylococcus aureus in samples of artisanal Coalho cheese produced with raw goat's milk in the state of Pernambuco, Brazil. In addition to evaluating the agreement between the official technique of Normative Instruction nº62/2003 (MAPA) and the molecular technique (nuc gene) to identify S. aureus in cheese. There was growth of typical Staphylococcus aureus colonies in 100% of the samples and the count ranged from 7.0×103 to 8.6×106 CFU/g. Of the 30 analyzed samples, 18 (60.0%) presented values greater than or equal to 105CFU/g and 21 (70.0%) were contaminated by S. aureus. The agreement between the diagnostic methods of S. aureus in goat cheese was moderate. The level of contamination of cheeses reveals the need for actions to improve the preparation conditions of the product in order to guarantee a safe product to consumers.(AU)


Subject(s)
Staphylococcus aureus/isolation & purification , Cheese/microbiology , Milk/microbiology , Refrigeration , Brazil , Goats , Foodborne Diseases
14.
Medisan ; 24(2)mar.-abr. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1098393

ABSTRACT

Introducción: Las enfermedades transmitidas por alimentos se producen por ingestión de un alimento, incluido el agua, que puede estar contaminado por diversos agentes. Objetivo: Caracterizar los agentes bacterianos aislados en brotes de enfermedades transmitidas por alimentos. Métodos: Se realizó un estudio observacional, descriptivo y transversal de 100,0 % de los brotes de enfermedades transmitidas por alimentos en la provincia de Santiago de Cuba, desde enero de 2018 hasta diciembre de 2019, para lo cual se seleccionaron muestras de alimentos y heces fecales. La caracterización de las bacterias aisladas se basó en los resultados del crecimiento y otras pruebas bioquímicas-metabólicas. Se utilizaron resultados del aislamiento y confirmación de los agentes identificados en cada uno de los brotes a partir de las muestras antes citadas. Entre las variables analizadas figuraron: número de brotes, muestras de alimentos, de heces fecales y resultados de pruebas bioquímicas y metabólicas. Resultados: Se obtuvo un aislamiento de agentes bacterianos en 100,0 % de las muestras de alimentos. Hubo una mayor frecuencia de bacterias Gram negativas (82,0 %) y la menor correspondió a microorganismos Gram positivos (18,0 %). La Salmonella D fue el microorganismo más frecuente. Conclusiones: Este resultado representa un instrumento para el diagnóstico etiológico de los brotes de enfermedades transmitidas por alimentos en Santiago de Cuba.


Introduction: Diseases transmitted by foods are produced due to ingestion of a food, including water that can be contaminated by diverse agents. Objective: To characterize the bacterial agents isolated in diseases outbreaks transmitted by foods. Methods: An observational, descriptive and cross-sectional study of 100.0 % of the diseases outbreaks transmitted by foods in Santiago de Cuba, from January, 2018 to December, 2019 was carried out, for which samples of foods and stools were selected. The characterization of the isolated bacterias was based on the results of growth and other biochemical-metabolic tests. Results of the isolation and confirmation of agents identified in each one of the outbreaks from the samples mentioned above were used. Among the analyzed variables we can mention: number of outbreaks, samples of foods, samples of stools and results of biochemical and metabolic tests. Results: An isolation of bacterial agents was obtained in 100.0 % of foods samples. There was a higher frequency of Gram negative bacterias (82.0 %) and the lower corresponded to Gram positive microorganisms (18.0 %). Salmonella D was the most frequent microorganism. Conclusions: This result represents an instrument for the etiological diagnosis of diseases outbreaks transmitted by foods in Santiago de Cuba.


Subject(s)
Bacteria , Culture Media , Foodborne Diseases/epidemiology , Disease Outbreaks
15.
Rev. epidemiol. controle infecç ; 10(1): 15-20, jan.-mar. 2020. ilus
Article in Portuguese | LILACS | ID: biblio-1177737

ABSTRACT

Justificativa e Objetivos: A praticidade de fazer refeições fora de casa representa um risco à saúde do consumidor, especialmente pela exposição a possíveis agentes causadores de doenças presentes nos alimentos. Isto ocorre devido às condições higiênico-sanitárias, geralmente precárias, o que aumenta a probabilidade de contaminação microbiana desses alimentos. Assim, o presente estudo possui como objetivo verificar a presença de coliformes totais e termotolerantes e de Staphylococcus spp. em amostras das mãos de manipuladores de lanches de rua. Métodos: A investigação de microrganismos foi realizada de duas formas: a) coleta dos microrganismos presentes nas mãos de vendedores ambulantes, o que está diretamente associado a doenças transmitidas por alimentos, e b) aplicação de um questionário com a finalidade de verificar informações sobre as condições de higiene dos manipuladores. Resultados: Através das análises microbiológicas, foram identificadas a presença de coliformes totais e de Escherichia coli, bem como a presença de Staphylococcus coagulase positiva (S. aureus) e coagulase negativa. Conclusão: As condições de higiene dos manipuladores se mostraram insatisfatórias conforme os resultados apresentados na análise e no questionário aplicado para esses indivíduos.(AU)


Background and Objectives: The convenience of eating meals outside home poses a risk to consumers' health, especially due to the exposure to possible disease-causing agents present in food. This is a result of generally poor hygienic-sanitary conditions, which increases the likelihood of microbial contamination of these foods. Thus, the aim of this study is to investigate the presence of total and thermotolerant coliforms and Staphylococcus spp. in samples taken from the hands of street food handlers. Methods: Two approaches were used in this investigation: a) the collection of microorganisms present in the hands of street vendors, which is directly associated with foodborne diseases, and b) the conduction of a questionnaire to gather information on the hygiene conditions of the handlers. Results: The microbiological analyses identified the presence of total coliforms and Escherichia coli, as well as Staphylococcus coagulase-positive (S. aureus) and coagulase-negative. Conclusion: The hygiene conditions of the handlers were considered unsatisfactory according to the results presented in the analysis and the questionnaire applied to the subjects.(AU)


Justificación y Objetivos: La practicidad de comer fuera de casa presenta un riesgo para la salud del consumidor, especialmente por la exposición a posibles agentes causantes de enfermedades alimentarias. Esto se debe a las condiciones higiénico-sanitarias, generalmente precarias, que aumenta la probabilidad de contaminación microbiana de esos alimentos. El presente estudio tuvo como objetivo verificar la presencia de coliformes totales y termotolerantes de Staphylococcus spp. en muestras de las manos de manipuladores de alimentos en la calle. Métodos: Se realizó el análisis de microorganismos de dos maneras: a) se recolectó la presencia del microorganismo presente en las manos de los vendedores ambulantes, lo que está directamente asociado a las enfermedades transmitidas por alimentos; y b) se aplicó un cuestionario con el fin de recoger informaciones sobre las enfermedades y condiciones de higiene de los manipuladores. Resultados: Se identificó la presencia de coliformes totales y de Escherichia coli, así como la presencia de Staphylococcus coagulase positivo (S. aureus) y coagulase negativo. Conclusión: Las condiciones de higiene de los manipuladores se mostraron insatisfactorias conforme los resultados presentados en el análisis y en el cuestionario aplicado a esos individuos.(AU)


Subject(s)
Humans , Hygiene , Microbiological Techniques , Street Food , Foodborne Diseases , Staphylococcus
16.
Arq. Inst. Biol ; 87: e0812019, 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1130055

ABSTRACT

Food prepared with products derived from animals are involved in most cases of staphylococcal poisoning; therefore, the research of Staphylococcus spp. in Emmental cheese is more applicable. The objective of this study was to identify coagulase-negative Staphylococcus spp. (CNS) in cheese using biochemical and molecular techniques to detect the presence of nine genes responsible for the production of enterotoxins. From 180 samples analyzed, 204 CNS strains were obtained and identified as being 46 (22.6%) S. saprophyticus strains, 27 (13.2%) S. hominis spp. hominis strains, 22 (10.8%) S. sciuri strains, 21 (10.3%) S. xylosus strains, 19 (9.3%) S. epidermidis strains, 19 (9.3%) S. haemolyticus strains, 17 (8.3%) S. lentus strains, 17 (8.3%) S. warneri strains, 11 (5.4%) S. equorum strains and 5 (2.5%) S. cohnni . Using the PCRm protocol, 14 (6.9%) strains with the presence of the genes on the enterotoxin E (SEE)11 (78.6%), J (SEJ) 1 (7%), C (SEC) 1 (7%) and I (SEI) 1 (7%) were detected. Based on the results, the type of package is not interfered of growth and isolated that Staphylococcus spp. in cheese. It was observed that bacteria capacity to produce coagulase cannot be understood as an indicative of enterotoxigenicity; therefore, the CNS should be considered as a target of importance in the epidemiology of staphylococcal intoxications. It can be concluded that CNS need to be included in bacterial foodborne disease research, since the genes responsible for the production of toxins were detected and none of the studied samples presented Staphylococcus spp. counting above the limits allowed by legislation.(AU)


Os alimentos preparados com produtos de origem animal são os mais envolvidos em casos de intoxicação alimentar estafilocócica; portanto a pesquisa do Staphylococcus spp. em queijos tipo Emmental é relevante. O objetivo foi isolar e identificar espécies de Staphylococcus coagulase negativas (CNS)de queijo Emmental acondicionado em vários tipos de embalagem, por meio de técnicas bacteriológicas e bioquímicas e detectar, por PCR, a presença de nove genes responsáveis pela produção de enterotoxinas. Das 180 amostras, foram isoladas 204 cepas de CNS, que foram identificadas por provas bioquímicas como: 46 (22,6%) S. saprophyticus, 27 (13,2%) S. hominis spp. hominis, 22 (10,8%) S. sciuri, 21 (10,3%) S. xylosus, 19 (9,3%) S. epidermidis , 19 (9,3%) S. haemolyticus , 17 (8,3%) S. lentus , 17 (8,3%) S. warneri , 11(5,4%) S. equorum e 5 (2,5%) S. cohnii . Na PCR multiplex, em 14 (6,9%) isolados foi detectada a presença dos genes para enterotoxina E (SEE), em 11 (78,6%) J (SEJ), em 1 (7%) C (SEC) e em 1 (7%) I (SEI). Com base nos resultados, o tipo de embalagem não interferiu na multiplicação dos Staphylococcus spp. isolados dos queijos. Neste estudo, verificou-se que a capacidade para a produção de coagulase pela bactéria não pode ser concebida como indicativa de enterotoxigenicidade, portanto devem-se considerar os CNS como objeto de importância na epidemiologia das intoxicações estafilocócicas, fazendo-se necessária a atenção com relação à pesquisa dos CNS nos alimentos, uma vez que foram detectados genes responsáveis pela produção de toxinas, e nenhuma das amostras apresentou contagem para Staphylococcus spp. acima do limite permitido pela legislação.(AU)


Subject(s)
Staphylococcal Food Poisoning , Staphylococcus/virology , Enterotoxins , Foodborne Diseases , Bacteria , Cheese , Polymerase Chain Reaction , Bacteriological Techniques , Product Packaging , Foods of Animal Origin , Food Safety , Food Supply
17.
Article in Spanish | LILACS, BINACIS, ARGMSAL | ID: biblio-1141558

ABSTRACT

INTRODUCCIÓN: La inseguridad alimentaria (IA) es un fenómeno multidimensional, que obliga a las personas a reducir la cantidad o calidad de los productos que consumen y presenta un contínuum de niveles de gravedad (de leve a severa). El objetivo fue analizar la relación entre IA y estado nutricional en madres de niños concurrentes a Centros de Atención Primaria de Salud de cuatro provincias del Noroeste de Argentina. MÉTODOS: Se aplicó un cuestionario con el indicador de insuficiencia alimentaria de la USDA y NHANES III a 995 madres. El estado nutricional se evaluó mediante el índice de masa corporal (IMC) y la circunferencia de cintura. RESULTADOS: La prevalencia de obesidad (IMC >30 kg/m2 ) fue mayor entre las participantes con IA severa (37,1%) y moderada (28,7%) que en aquellas con IA leve (20,3%) o con seguridad alimentaria (SA) (22,4%) (p 0,003). En modelos de regresión logística multivariada la IA severa incrementaba la probabilidad de IMC ≥30 kg/m2 (OR 1,8) y de circunferencia de cintura >80 cm (OR 1,8). Tener bajos niveles de ingresos y de educación, estar casada o en pareja y consumir asiduamente comidas fritas también constituían factores de riesgo significativos. DISCUSIÓN: Las políticas alimentarias deben combinarse con políticas de empleo y de distribución de ingresos, de producción y acceso a alimentos saludables, junto a legislación sobre la composición y publicidad de alimentos y bebidas, y a la diseminación de información y de buenas prácticas alimentarias.


Subject(s)
Women , Food Assistance , Foodborne Diseases , Latin America , Obesity
18.
Rev. Soc. Bras. Med. Trop ; 53: 1-4, dez., 2019. ilus.
Article in English | SES-SP, LILACS, SES-SP, SESSP-IDPCPROD, SES-SP | ID: biblio-1049465

ABSTRACT

INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples. (AU)


Subject(s)
Coleoptera , Polymerase Chain Reaction , Triatominae , Euterpe , Food , Foodborne Diseases
19.
Rev. salud pública ; 21(6): e169898, Nov.-Dec. 2019. tab
Article in English | LILACS | ID: biblio-1099277

ABSTRACT

ABSTRACT Objective This study aimed to determine the dietary habits related to fish consumption and the risk factors associated with acquiring an ichthyo-zoonotic disease. Materials and Methods A descriptive, cross-sectional study was carried out by means of a structured survey administered to 150 individuals in the city of Cali, Colombia. Results Epidemiological variables regarding fish consumption and preparation were contrasted with the medical records of the respondents. The median fish consumption in the surveyed population was three times a month, with raw or salted/marinated fish once a month. A positive correlation between fish consumption and allergic conditions was confirmed. There was no infectious or parasitic history associated with the data on fish consumption. Conclusions A relationship between fish consumption and allergies was confirmed. Further research is necessary to establish the possible pathogens associated with hypersensitivity, such as parasites of the Anisakidae family.(AU)


RESUMEN Objetivo Este estudio tuvo como fin determinar los hábitos alimenticios relacionados con el consumo de pescado y los factores de riesgo asociados con la adquisición de una enfermedad ictio-zoonótica. Materiales y Métodos Se realizó un estudio descriptivo de corte transversal mediante una encuesta estructurada a 150 individuos de la ciudad de Cali, Colombia. Resultados Las variables epidemiológicas del consumo y preparación de pescado se relacionaron con la historia clínica de los encuestados. El consumo medio de pescado en los encuestados fue de tres veces al mes, con pescado crudo o salado/marinado una vez al mes. Se confirmó una correlación positiva entre el consumo de pescado y las condiciones alérgicas. No se asociaron antecedentes infecciosos o parasitarios con los datos sobre el consumo de pescado. Conclusión Fue posible confirmar una relación entre el consumo de pescado y las condiciones alérgicas. Se necesita investigación para establecer los posibles patógenos asociados con la hipersensibilidad, como los parásitos de la familia Anisakidae.(AU)


Subject(s)
Humans , Zoonoses/etiology , Food Hypersensitivity/etiology , Foodborne Diseases/epidemiology , Epidemiology, Descriptive , Cross-Sectional Studies , Cohort Studies , Colombia
20.
Rev. argent. microbiol ; 51(4): 354-358, dic. 2019. graf
Article in Spanish | LILACS | ID: biblio-1057400

ABSTRACT

Resumen El 27 de noviembre de 2008 ocurrió un brote de intoxicación alimentaria asociado al consumo de salpicón de ave en un jardín de infantes de Hurlingham, provincia de Buenos Aires. Treinta y siete niños y 10 adultos presentaron síntomas gastrointestinales. Cinco niños fueron internados con signos de deshidratación, y uno de ellos requirió cuidados intensivos. Se aisló Staphylococcus aureus subsp. aureus del alimento involucrado, de 4/5 muestras de materia fecal de pacientes y de 3/5 manipuladores (nariz del manipulador 1, manos de manipuladores 2 y 3). Las cepas aisladas portaban los genes que codifican las enterotoxinas SEA y SED. Por electroforesis de campo pulsado con la enzima SmaI, los patrones de macrorrestricción presentaron 100% de similitud. La investigación oportuna del brote permitió identificar al agente causal de la intoxicación, determinar las fallas en la elaboración del alimento e implementar las medidas correctivas correspondientes.


Abstract On November 27, 2008, a foodborne disease outbreak associated with the consumption of chicken salad occurred in a kindergarten in the District of Hurlingham, Province of Buenos Aires. Thirty-seven children and 10 adults with gastrointestinal symptoms were affected. Five children were hospitalized with signs of dehydration, one of them requiring intensive care. Staphylococcus aureus subsp. aureus was isolated from the mentioned food in 4 out of 5 stool specimens from the patients, and in 3 out of 5 food handlers (nose of food handler #1, hands of food handlers #2 and 3). The isolates carried the genes coding for enterotoxins SEA and SED. The macrorestriction patterns showed 100% similarity by pulsed-field gel electrophoresis using the SmaI enzyme. A timely outbreak investigation allowed us to identify the causative agent of the food poisoning as well as the failures in food processing and to implement corrective measures.


Subject(s)
Poisoning/etiology , Staphylococcus aureus/isolation & purification , Enterotoxins/analysis , Foodborne Diseases/diagnosis , Electrophoresis, Gel, Pulsed-Field/methods
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