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1.
Braz. arch. biol. technol ; 64: e21190643, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249204

ABSTRACT

Abstract The aim of this study was to estimate allelic and genotypic frequencies of markers in the leptin (LEP), pituitary transcription factor (PIT-1) and luteinizing hormone receptor (LHR) genes and evaluate their effects on reproductive traits and milk yield of Holstein cattle. Data from 147 cows from department of Francisco Morazán, Honduras, were collected and PCR-Restriction Fragment Length Polymorphism (RFLP) assays were performed to characterize the PIT-1-HinfI, LEP- A59V and LHR-rs41256848 polymorphisms. To estimate the effect of genotypes on reproductive traits and milk yield fixed and mixed linear models were fitted. The frequencies of the genotypes CC, CT and TT of A59V, AA, AB and BB of HinfI, and CC, CG and GG of rs41256848 were 0.46, 0.33 and, 0.21; 0.09, 0.32 and 0.58; and 0.37, 0.61 and 0.02, respectively. The genotypes of LEP and LHR showed deviations from Hardy-Weinberg equilibrium. The A59V polymorphism was significantly associated with the calving to conception interval (CCI) (p=0.01), being the C allele favorable. The HinfI and rs41256848 polymorphism were significantly associated (p=0.08 and p=0.04) with age to first calving (AFC), being the A and G the alleles favorable associated, respectively. The results suggest that LEP, PIT and LHR polymorphisms can probably act as candidate to be used in marker-assisted selection for AFC and CCI traits.


Subject(s)
Luteinizing Hormone , Leptin , Genetic Profile , Gene Frequency/physiology , Reproduction , Cattle , Polymerase Chain Reaction/instrumentation
2.
Article in English | AIM, AIM | ID: biblio-1342268

ABSTRACT

Sickle Cell Disease (SCD) is prevalent in Nigeria with 150,000 new cases yearly, owing to poor knowledge, poverty and lack of screening. This study investigated knowledge and willingness to undergo genotype screening among young people who might be contemplating marriage or reproduction during or soon after the National Youth Service Corps (NYSC); the compulsory one-year service for all Nigerian graduates from tertiary institutions. The study was a descriptive cross-sectional design, carried out amongst 355 respondents using a stratified random sampling. Both qualitative and quantitative methods were employed with due ethical considerations and analysis. The age range was between 18 and 30 years, with more female (54.6%) respondents. Almost all (96.3%) of the respondents have heard about SCD. The prevalence of SCD in the families of the respondents was 10.1% and majority (87.0%) have had genotype screening. Many (60.6%) had good knowledge about SCD but less than half (44.5%) could state the difference between genotype and blood group. Statistical associations were recorded between level of knowledge and gender, discipline of study and willingness. Concerted efforts should be geared towards SCD health education and screening using the platform of corps members' during their orientation and weekly community development programmes.


Subject(s)
Humans , Mass Screening , Anemia, Sickle Cell , Knowledge , Genetic Profile
3.
Rev. Bras. Saúde Mater. Infant. (Online) ; 20(2): 467-471, Apr.-June 2020. tab
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1136441

ABSTRACT

Abstract Objectives: the present study aimed to evaluate the association between the rs1799998 polymorphism of the CYP11B2 gene and the susceptibility to preeclampsia (PE) in a Brazilian population. Methods: the study group comprised 61 women who were diagnosed with PE. The control group included 116 women who did not show changes in their blood pressure levels during their pregnancies. The rs1799998 polymorphism of the CYP11B2 gene was amplified by allele-specific polymerase chain reaction (PCR). A multiple logistic regression analysis was performed using the SNPStat program to evaluate the risk of the CYP11B2 gene rs1799998 polymorphism contributing to PE. Results: the PE group had the following genotypes: 1.64% CC, 91.80% CT, and 6.56% TT. In the control group, the observed genotypic frequencies were: 11% CC, 73% CT, and 16% TT. The genotypic frequency distribution did not fit the Hardy Weinberg Equilibrium (HWE) in either study group. The multiple logistic regression analysis showed a statistically significant difference for the rs1799998 polymorphism in the recessive model. Conclusion: the results suggest an association between the recessive model of C/C genotype of the rs1799998 polymorphism of the CYP11B2 gene and susceptibility to PE.


Resumo Objetivos: avaliar a associação entre o polimorfismo rs1799998 do gene CYP11B2 e a suscetibilidade à PE em uma população brasileira. Métodos: participaram desse estudo 61 mulheres com PE e 116 mulheres normotensas. O polimorfismo rs1799998 do gene CYP11B2 foi amplificado por PCR alelo-específica. O risco do polimorfismo rs1799998 do gene CYP11B2 contribuir com a PE foi avaliado pela análise de regressão logística múltipla. Resultados: as frequências genotípicas observadas foram 1.64% CC, 91.80% CT e 6.56% TT no grupo PE e 11%CC, 73%CT e 16%TT grupo controle. A distribuição da frequência genotípica não estava em Equilíbrio de Hardy Weinberg em nenhum dos grupos estudados. A análise de regressão logística múltipla demonstrou diferença estatisticamente significativa para o polimorfismo rs1799998 no modelo recessivo. Conclusão: o presente trabalho sugere associação do genótipo C/C no modelo recessivo, do polimorfismo rs1799998 do gene CYP11B2 com a suscetibilidade a PE.


Subject(s)
Humans , Female , Pregnancy , Polymorphism, Genetic , Pre-Eclampsia/genetics , Cytochrome P-450 CYP11B2 , Cytochrome P-450 Enzyme System , Brazil , Genetic Markers , Logistic Models , Genetic Predisposition to Disease , Genetic Profile
4.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 961-969, May-June, 2020. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1129665

ABSTRACT

A total of 6593 weight records collected from 796 male and female Anglo-Nubian goats aged up to 130 days, offspring from 29 sires and 225 dams, were used to compare models and estimate genetic parameters throughout the growth curve by applying random regression models. Direct and maternal additive genetic effects and direct and maternal permanent environmental effects were included as random in the models. The contemporary groups were included as fixed effects and goat age at kidding was included as a covariable (linear and quadratic). The choice of the best model was based on the AIC, BIC and AICc criteria. Variance estimates of the four random effects increased as the animals aged. Direct heritability (h2) rose from 0.13 to 0.40 with age, whereas maternal heritability showed a low value. Genetic correlations of weight between closer ages were high. The most suitable random regression model to compare the fitting of random effects was that which employed the Legendre polynomials of quadratic order with homogeneous variance (3333-1).(AU)


Utilizaram-se 6593 pesos de 796 caprinos da raça Anglonubiana, coletados em machos e fêmeas com idade até 130 dias, descendentes de 29 reprodutores e 225 matrizes, com o objetivo de se compararem modelos e de se estimarem parâmetros genéticos ao longo da curva de crescimento com aplicação de modelos de regressão aleatória. Nos modelos, incluíram-se os efeitos genéticos aditivos diretos e maternos e os de ambiente permanente diretos e maternos como aleatórios; os grupos de contemporâneos foram incluídos como efeitos fixos, e a idade da cabra ao parto como covariável (linear e quadrática). A escolha do melhor modelo foi realizada pela avaliação dos critérios AIC, BIC e AICc. As estimativas de variâncias dos quatro efeitos aleatórios cresceram de acordo com o aumento da idade. A herdabilidade direta (h2) aumentou de 0,13 a 0,40 com a idade, e a materna apresentou baixo valor. As correlações genéticas do peso entre idades mais próximas foram altas. O modelo de regressão aleatório mais adequado ao se comparar o ajuste dos efeitos aleatórios foi o que empregou polinômios de Legendre de ordem quadrática com variância homogênea (3333-1).(AU)


Subject(s)
Animals , Ruminants/growth & development , Regression Analysis , Genetic Profile , Heredity , Correlation of Data
5.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 970-976, May-June, 2020. ilus, tab
Article in English | LILACS, VETINDEX | ID: biblio-1129701

ABSTRACT

This study aimed to establish criteria for eliminating redundant variables, to know the magnitude of the data relationship, and to provide information that helps researchers in the use of the technique to analyze and interpret production data and egg quality. The data used in this work was obtained from four successive generations of the quail lineage developed by the Department of Animal Science of the Federal University of Pelotas. The characteristics were measured from the 42nd day of age, when the egg production period began, until 126 days of production, obtaining three 28 day periods (cycles) in the four successive generations, totaling 545 females. Of the twelve original variables, only seven demonstrated potential to be maintained in future experiments, representing a 42% exclusion. The main philosophy of this study was the analysis of the studied variables and made possible the understanding of the relationship and the correlations.(AU)


O objetivo do presente trabalho foi estabelecer critérios para eliminação de variáveis redundantes, conhecer a magnitude das relações dos dados, além de fornecer informações que auxiliem pesquisadores na utilização da técnica para analisar e interpretar dados de produção e qualidade de ovos de codornas. Os dados utilizados neste trabalho são provenientes de quatro gerações sucessivas da linhagem de codornas de corte desenvolvida pelo Departamento de Zootecnia da Universidade Federal de Pelotas. As características foram mensuradas do 42º dia de idade até o 126º dia de produção, totalizando 545 fêmeas. Das 12 variáveis originais analisadas, apenas sete demonstraram potencial para serem mantidas em experimentos futuros, representando uma exclusão de 42%. A análise de componentes principais foi efetiva para a redução das variáveis estudadas e possibilitou a compreensão da relação e correlação dessas.(au)


Subject(s)
Animals , Coturnix , Eggs/analysis , Genetic Profile , Food Quality , Multivariate Analysis
7.
Mem. Inst. Oswaldo Cruz ; 115: e190413, 2020. tab, graf
Article in English | LILACS | ID: biblio-1101274

ABSTRACT

BACKGROUND The leishmaniases are complex neglected diseases caused by protozoan parasites of the genus Leishmania. Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis in the New World. In recent studies, genomic changes such as chromosome and gene copy number variations (CNVs), as well as transcriptomic changes have been highlighted as mechanisms used by Leishmania species to adapt to stress situations. OBJECTIVES The aim of this study was to determine the effect of short-term minor temperature shifts in the genomic and transcriptomic responses of L. braziliensis promastigotes in vitro. METHODS Growth curves, genome and transcriptome sequencing of L. braziliensis promastigotes were conducted from cultures exposed to three different temperatures (24ºC, 28ºC and 30ºC) compared with the control temperature (26ºC). FINDINGS Our results showed a decrease in L. braziliensis proliferation at 30ºC, with around 3% of the genes showing CNVs at each temperature, and transcriptomic changes in genes encoding amastin surface-like proteins, heat shock proteins and transport proteins, which may indicate a direct response to temperature stress. MAIN CONCLUSIONS This study provides evidence that L. braziliensis promastigotes exhibit a decrease in cell density, and noticeable changes in the transcriptomic profiles. However, there were not perceptible changes at chromosome CNVs and only ~3% of the genes changed their copies in each treatment.


Subject(s)
Animals , Temperature , Leishmania braziliensis/genetics , Adaptation, Physiological/genetics , DNA Copy Number Variations/genetics , Transcriptome/genetics , Adaptation, Physiological/physiology , Gene Expression Profiling , Genetic Profile
8.
Rev. argent. microbiol ; 51(1): 84-92, mar. 2019. ilus, tab
Article in Spanish | LILACS | ID: biblio-1041820

ABSTRACT

El complejo Burkholderia cepacia está formado por 22 especies conocidas como patógenos oportunistas en personas inmunocomprometidas, especialmente en aquellas con fibrosis quística. También se aíslan de infecciones nosocomiales y son difíciles de erradicar debido a su capacidad intrínseca para resistir una gran variedad de antibióticos. En general, estas especies presentan genomas de gran tamaño (hasta 9 Mpb) divididos en 2-5 replicones. Esta característica aporta una gran versatilidad metabólica, que se considera importante para habitar el suelo, el agua, las plantas, incluso los nódulos en leguminosas. Algunas especies del complejo B. cepacia exhiben actividades benéficas, como biorremediación, biocontrol y promoción del crecimiento vegetal. No obstante, debido a su papel en infecciones de humanos, su uso en la agricultura está restringido. El complejo B. cepacia es un tema constante de estudio debido a su impacto en el sector salud y su potencial en la agricultura. En este trabajo se examina la historia del complejo B. cepacia y se revisa la información reciente relacionada con este grupo de bacterias.


The Burkholderia cepacia complex is a group of 22 species, which are known as opportunistic pathogens in immunocompromised people, especially those suffering from cystic fibrosis. It is also found in nosocomial infections and is difficult to eradicate due to intrinsic resistance to several antibiotics. The species have large genomes (up to 9 Mbp), distributed into 2-5 replicons. These features significantly contribute to genome plasticity, which makes them thrive in different environments like soil, water, plants or even producing nodules in legume plants. Some B. cepacia complex species are beneficial in bioremediation, biocontrol and plant-growth promotion. However, because the B. cepacia complex is involved in human infection, its use in agriculture is restricted. B. cepacia complex is being constantly studied due to the health problems that it causes and because of its agricultural potential. In this review, the history of B. cepacia complex and the most recently published information related to this complex are revised.


Subject(s)
Burkholderia cepacia complex/classification , Burkholderia cepacia complex/pathogenicity , Genetic Profile , Phenotype , Opportunistic Infections/microbiology , Sequence Analysis, DNA/methods , Burkholderia Infections/epidemiology
9.
Odovtos (En línea) ; 20(3): 105-113, Sep.-Dec. 2018. tab, graf
Article in Spanish | LILACS, BBO | ID: biblio-1091464

ABSTRACT

RESUMEN La caries es una de las enfermedades de naturaleza infecciosa, crónica transmisible muy prevalente en el Perú, relacionada a la presencia del Streptococcus mutans, los hábitos de higiene y nutricionales. Objetivo: El propósito de este estudio fue determinar la presencia del genotipo C en el Streptococcus mutans en niños y adolescentes peruanos, utilizando la técnica PCR- Multiplex; y su asociación con la prevalencia de caries dental. Material y método: Se trabajó con una muestra de 78 niños y adolescentes de ambos sexos de Lima. El estudio consistió en dos fases, en la primera se obtuvo la saliva estimulada, para el cultivo bacteriano, las mismas que fueron sembradas en agar Mitis Salivarius con bacitracina y sulfisoxasol. En la segunda fase se realizó la genotipificación de acuerdo con su perfil enzimático. Para la extracción de ADN se utilizó el GF-1 Bacterial DNA Extraction Kit de GeneONGmbH para lo cual se realizó cultivos de las cepas de Streptococcus sp en el caldo BHI con sacarosa a 37ºC por 24 horas. Resultados: Se evidencia la presencia de Streptococcus mutans en 75.6%: 59 de 78 muestras de saliva. Los resultados de la genotipificación por PCR Multiplex demuestran la presencia de 22 muestras de saliva de Streptococcus mutans con genotipos C (37,29%) y 37 muestras (62,71%) que no pertenecen a dicho Genotipo. Conclusiones: Los resultados evidenciaron que el Streptococcus mutans genotipo C no está relacionado al sexo, grupo etario ni a la presencia de caries dental.


ABSTRACT Caries is one of the diseases of infectious nature, chronic transmissible very prevalent in Peru, related to the presence of Streptococcus mutans, hygienic and nutritional habits. Objective: The purpose of this study was to determine the presence of genotype C in Streptococcus mutans in Peruvian children and adolescents, using the PCR-Multiplex technique; and its association with the prevalence of dental caries. Materials and methods: The study was done with a sample of 78 children and adolescents of both sexes from Lima. The study consisted of two phases, on the first one the stimulated saliva was obtained, for the bacterial culture, the same ones that were grown on Mitis Salivarius agar with bacitracin and sulfisoxasol. On the second phase, genotyping was carried out according to its enzymatic profile. For the extraction of DNA, the Gene Extraction Kit GG-1 Bacterial DNA was used, for which cultures of Streptococcus sp strains were performed in the BHI broth with sucrose at 37ºC for 24 hours. Results: The presence of Streptococcus mutans was evidenced in 59 (75.6%) of 78 saliva samples. The results of the genotyping by PCR Multiplex demonstrate the presence of 22 saliva samples of Streptococcus mutans with genotypes C (37,297%) and 37 samples 62, 71 % without this Genotype. Conclusions: The results showed that the presence of genotype C is not related to sex, age group or the presence of dental caries.


Subject(s)
Humans , Male , Female , Child , Adolescent , Streptococcus mutans/pathogenicity , Dental Caries/etiology , Multiplex Polymerase Chain Reaction/methods , Genetic Profile , Peru
10.
Article in Spanish | LILACS, BIMENA | ID: biblio-1348771

ABSTRACT

Este artículo expone los conceptos básicos de las pruebas de ADN en el contexto forense. Describe como se establece la coincidencia entre el perfil genético de la muestra biológica encontrado en la escena del crimen con el perfil de un sospechoso o imputado; como se estima la probabilidad de que la coincidencia haya sido por azar evaluando la frecuencia del perfil genético en la población, es decir, la probabilidad de que el sospechoso en realidad no haya sido la fuente de la evidencia; y otros parámetros estadísticos importantes al momento de evaluar un análisis de ADN...(AU)


Subject(s)
Humans , Forensic Genetics , Genetic Profile , DNA , Genetic Markers , Data Interpretation, Statistical
11.
J. coloproctol. (Rio J., Impr.) ; 37(2): 163-170, Apr.-June 2017. graf
Article in English | LILACS | ID: biblio-893974

ABSTRACT

ABSTRACT Introduction: Desmoid tumors are the main extraintestinal manifestation of FAP, presenting high morbidity and mortality. It is a neoplasia without metastasis capacity, but with infiltrative growth and with a high rate of recurrence. In familial forms, these tumors are associated with a germinal mutation in the APC gene, with a genotype-phenotype correlation influenced by other risk factors. Materials and methods: A review of articles published since the year 2000 in Portuguese, English or Spanish on desmoid tumors in patients with FAP was carried out. A total of 49 publications were included. Results: The site of the mutation in the APC gene is related to the severity of FAP and to the frequency of desmoid tumor. Mutations located distally to codon 1309 are associated with a more attenuated polyposis, but with higher frequency of desmoid tumors. Clinically, these tumors may or may not be symptomatic, depending on their size and location. In their treatment, priority should be given to medical therapy, especially in intra-abdominal tumors, with surgery being the last option if there are no other complications. Discussion: These tumors are associated with certain risk factors: genetic (mutation site), hormonal (estrogenic environment) and physical (surgical trauma) ones. In young women, a later prophylactic colectomy is suggested. Moreover, the laparoscopic approach to prophylactic surgery seems to be an option that reduces surgical trauma and consequently the appearance of desmoid tumors. Conclusion: The step-up medical approach has been shown to be valid in the treatment of intra-abdominal desmoid tumors, and medical treatment should be the first therapeutic option.


RESUMO Introdução: Os tumores desmóides são a principal manifestação extraintestinal da PAF, apresentando elevada morbimortalidade. É uma neoplasia sem capacidade de metastização, mas com crescimento infiltrativo e com alta taxa de recorrência. Nas formas familiares associa-se a uma mutação germinativa no gene APC, havendo uma correlação genótipo-fenótipo influenciada por outros fatores de risco. Materiais e métodos: Foi efetuada uma revisão de artigos publicados desde o ano 2000, em português, inglês ou espanhol, acerca de tumores desmóides em doentes com PAF. Foram incluídas, no total, 49 publicações. Resultados: O local da mutação no gene APC relaciona-se com a gravidade da PAF e frequência de tumor desmóide. Mutações localizadas distalmente ao codão 1309 associam-se a uma polipose mais atenuada, mas a maior frequência de tumor desmóide. Clinicamente podem ser, ou não, sintomáticos, dependendo do seu tamanho e localização. No seu tratamento deve ser dada prioridade à terapêutica médica, sobretudo nos tumores intra-abdominais, colocando a cirurgia como última opção, caso não hajam outras complicações. Discussão: Estes tumores associam-se a determinados fatores de risco: genéticos (local da mutação), hormonais (ambiente estrogénico) e físicos (trauma cirúrgico). Nas mulheres jovens sugere-se a realização de colectomia profilática mais tardiamente. Além disso, a abordagem laparoscópica para a cirurgia profilática parece ser uma opção que diminui o trauma cirúrgico e consequentemente o aparecimento de tumores desmóides. Conclusão: A abordagem médica em step-up mostrou ser válida no tratamento de tumores desmóides intra-abdominais, devendo o tratamento médico ser a primeira opção terapêutica.


Subject(s)
Humans , Fibromatosis, Aggressive/pathology , Adenomatous Polyposis Coli/pathology , Genetic Profile
12.
Rev. argent. microbiol ; 48(1): 62-66, mar. 2016. graf.
Article in English | LILACS | ID: biblio-1284344

ABSTRACT

En este estudio fueron analizadas mediante el cultivo muestras de orina de pacientes hospitalizados en la región centro-oeste de Brasil; los microorganismos aislados fueron identificados filogenéticamente como Trichosporon asahii. A través del análisis de máxima parsimonia de las secuencias de IGS1, fueron encontrados 3 genotipos que no habían sido descritos anteriormente. Las concentraciones inhibitorias mínimas frente a los 9 aislados identificados presentaron un rango de 0,06-1µg/ml en el caso de la anfotericina B, de 0,25-4µg/ml en el del fluconazol, y de 0,03-0,06µg/ml en el del itraconazol. Aproximadamente 6/9 de los aislados de T. asahii formaron biopelículas en la superficie de microplacas de poliestireno. Este trabajo documenta el aislamiento de T. asahii como agente causal de infeciones urinarias nosocomiales. Además, demuestra que la región IGS1 puede ser considerada una nueva herramienta epidemiológica para la genotipificación de los aislados de T. asahii. Los genotipos menos comunes encontrados en este estudio pueden estar relacionados con las características epidemiológicas locales


In this study, the culture analysis of urine samples from patients hospitalized in the Central-West region of Brazil was performed, and the isolated microorganisms were phylogenetically identified as Trichosporon asahii. Maximum parsimony analysis of the IGS1 sequences revealed three novel genotypes that have not been described. The minimum inhibitory concentrations of the nine isolates identified were in the range of 0.06­1µg/ml for amphotericin B, 0.25­4µg/ml for fluconazole, and 0.03­0.06µg/ml for itraconazole. Approximately 6/9 of the T. asahii isolates could form biofilms on the surface of polystyrene microplates. This study reports that the microorganisms isolated here as T. asahii are agents of nosocomial urinary tract infections. Furthermore, the IGS1 region can be considered a new epidemiological tool for genotyping T. asahii isolates. The least common genotypes reported in this study can be related to local epidemiological trends


Subject(s)
Humans , Male , Female , Urinary Tract Infections/microbiology , Trichosporon/isolation & purification , Trichosporon/classification , Microbial Sensitivity Tests/methods , Urine/microbiology , Trichosporonosis/epidemiology , Genetic Profile
13.
Rev. argent. microbiol ; 47(4): 282-294, dic. 2015. ilus, mapas, tab
Article in English | LILACS | ID: biblio-843135

ABSTRACT

This study aimed to determine the clonal relationship among 137 Streptococcus uberis isolates from bovine milk with subclinical or clinical mastitis in Argentina and to assess the prevalence and conservation of pauA and sua genes. This information is critical for the rational design of a vaccine for the prevention of bovine mastitis caused by S. uberis. The isolates were typed by random amplified polymorphic DNA (RAPD) analysis and by pulsed-field gel electrophoresis (PFGE). The 137 isolates exhibited 61 different PFGE types and 25 distinct RAPD profiles. Simpson's diversity index was calculated both for PFGE (0.983) and for RAPD (0.941), showing a high discriminatory power in both techniques. The analysis of the relationship between pairs of isolates showed 92.6 % concordance between both techniques indicating that any given pair of isolates distinguished by one method tended to be distinguished by the other. The prevalence of the sua and pauA genes was 97.8 % (134/137) and 94.9 % (130/137), respectively. Nucleotide and amino acid sequences of the sua and pauA genes from 20 S. uberis selected isolates, based on their PFGE and RAPD types and geographical origin, showed an identity between 95 % and 100 % with respect to all reference sequences registered in GenBank. These results demonstrate that, in spite of S. uberis clonal diversity, the sua and pauA genes are prevalent and highly conserved, showing their importance to be included in future vaccine studies to prevent S. uberis bovine mastitis.


Este estudio pretendió determinar la relación clonal entre 137 aislamientos de S. uberis obtenidos de leche de bovinos con mastitis clínica o subclínica en la Argentina, como así también la prevalencia y la conservación de los genes sua y PauA entre dichos aislamientos. Esta información es crítica para el diseño racional de una vacuna que prevenga la mastitis bovina por S. uberis. Los aislamientos se tipificaron molecularmente por amplificación al azar del ADN polimórfico (RAPD) y mediante electroforesis de campos pulsados (PFGE). Los 137 aislamientos mostraron 61 pulsotipos mediante PFGE y 25 tipos de RAPD diferentes. Los índices de Simpson calculados fueron 0,983 por PFGE y 0,941 por RAPD; esto evidencia el elevado poder discriminatorio de ambas técnicas. El análisis de la relación entre pares de aislamientos mostró un 92,6 % de concordancia entre ambas técnicas, lo que indica que cualquier par de aislamientos que fue distinguido por un método tendió a ser distinguido por el otro. La prevalencia de los genes sua y puaA fue del 97,8 % (134/137) y 94,9 % (130/137), respectivamente. Las secuencias de nucleótidos y de aminoácidos codificados por los genes sua y pauA de los 20 aislamientos de S. uberis seleccionados sobre la base de su tipo de PFGE y RAPD y origen geográfico tuvieron un porcentaje de identidad de entre 95 % y 100 % con respecto a todas las secuencias de referencia registradas en GenBank. Estos resultados demuestran que, a pesar de la diversidad clonal de S. uberis, los genes sua y pauA son prevalentes y están altamente conservados y deberían ser incluidos en futuros estudios de vacunas para prevenir mastitis bovina causada por S. uberis.


Subject(s)
Animals , Cattle , Streptococcal Infections/veterinary , Streptococcus/isolation & purification , Streptococcus/genetics , Mastitis, Bovine/prevention & control , Streptococcal Infections/immunology , Prevalence , Genetic Profile
14.
Rev. argent. microbiol ; 47(2): 108-111, June 2015.
Article in English | LILACS | ID: biblio-1147122

ABSTRACT

El objetivo del presente estudio fue evaluar las relaciones genotípicas entre 40 Streptococcus uberis aislados de mastitis bovina mediante la técnica de electroforesis de campos pulsantes (pulsed-field gel electrophoresis [PFGE]). Además, se investigó la asociación entre los patrones de PFGE y los perfiles de virulencia. Los aislamientos mostraron 17 patrones de PFGE. Se encontraron diferentes cepas dentro de los tambos y en los distintos tambos, y un bajo número de aislamientos dentro del mismo tambo compartieron un perfil idéntico de PFGE. No se encontró ninguna asociación entre los patrones de PFGE y los perfiles de virulencia. Sin embargo, la detección de cepas particulares en algunos tambos podría indicar que algunas de ellas son más virulentas que otras. Sería importante avanzar en las investigaciones para identificar nuevos genes relacionados con la virulencia que podrían contribuir a la capacidad infecciosa de estas cepas


The aim of this study was to evaluate the genotypic relationships among 40 Streptococcus uberis isolated from bovine mastitis by using pulsed-field gel electrophoresis (PFGE). Additionally, the association between PFGE patterns and virulence profiles was investigated. The isolates exhibited 17 PFGE patterns. Different strains were found within and among herds; however, a low number of isolates within the same herd shared an identical PFGE type. No association between PFGE patterns and virulence profiles was found. However, the detection of specific strains in some herds could indicate that some strains are more virulent than others. Further research needs to be undertaken to elucidate new virulence-associated genes that might contribute to the capability of these strains to produce infection


Subject(s)
Animals , Cattle , Streptococcus/isolation & purification , Virulence/genetics , Electrophoresis, Gel, Pulsed-Field/methods , Mastitis, Bovine/genetics , Streptococcus/classification , Genetic Profile
15.
Rev. cuba. med. trop ; 66(2): 263-272, Mayo.-ago. 2014.
Article in Spanish | LILACS, CUMED | ID: lil-731978

ABSTRACT

INTRODUCCIÓN: la búsqueda de alternativas al polimorfismo de la longitud de los fragmentos de restricción (RFLP, siglas en inglés) con la sonda IS 6110 en la genotipificación de Mycobacterium tuberculosis ha propiciado el desarrollo de la tipificación con número variable de repeticiones en tándem de unidades repetitivas interespaciadas de micobacterias (MIRU-VNTR, siglas en inglés). OBJETIVO: evaluar la diseminación de genotipos de M. tuberculosis en La Habana en 2009. MÉTODOS: se estudiaron 80 aislamientos procedentes de unidades de salud durante 2009 y se caracterizaron por tipificación MIRU-VNTR-15. Los genotipos se expresaron como códigos numéricos según el número de copias de cada MIRU-VNTR amplificado, y se analizaron con la herramienta bioinformática en línea MIRU-VNTR plus. Se utilizó MIRU-VNTR-24 como tipificación secundaria en los aislamientos agrupados por MIRU-VNTR-15. RESULTADOS: con MIRU-VNTR-15 se definieron 41 genotipos diferentes; entre ellos, 33 únicos (41,25 por ciento), y ocho que agruparon a 47 aislamientos (58,75 por ciento). La tipificación MIRU-VNTR-24 logró diferenciar sólo el 5 por ciento de éstos, disminuyendo el porcentaje de agrupamiento a 53,75 por ciento. CONCLUSIONES: el elevado agrupamiento encontrado sugirió transmisión reciente, lo que pudo tener influencia en la incidencia de tuberculosis en La Habana en 2009(AU)


INTRODUCTION: the search for alternatives to restriction fragment length polymorphism (RFLP) with the IS6110probe for the genotyping of Mycobacterium tuberculosis has paved the way for the development of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing (MIRU-VNTR). OBJECTIVE: evaluate the spread of M. tuberculosis genotypes in Havana in 2009. METHODS: eighty isolates obtained from healthcare centers during 2009 were examined and characterized by 15-loci MIRU-VNTR typing. The genotypes were expressed as numerical codes according to the copy number of each amplified MIRU-VNTR, and they were analyzed with the online bioinformatic tool MIRU-VNTR plus. 24-loci MIRU-VNTR was used for secondary typing of isolates grouped by 15-loci MIRU-VNTR. RESULTS: forty-one different genotypes were defined with 15-loci MIRU-VNTR. Of these, 33 were single (41.25 percent), whereas 8 clustered 47 isolates (58.75 percent). Only 5 percent of the latter could be differentiated by 24-loci MIRU-VNTR, lowering the percentage of clustering to 53.75 percent. CONCLUSIONS: the high clustering values revealed by the study suggest that transmission was recent. This may have had an influence on the incidence of tuberculosis in Havana in 2009(AU)


Subject(s)
Humans , Tuberculosis, Pulmonary/epidemiology , Bacterial Typing Techniques/methods , Cuba , Genetic Profile
16.
J. venom. anim. toxins incl. trop. dis ; 14(4): 703-718, 2008. ilus, tab
Article in English | LILACS, VETINDEX | ID: lil-500138

ABSTRACT

The present study aimed to analyze the genetic similarity between genomic profiles of thirteen Klebsiella oxytoca and seven Klebsiella pneumoniae samples isolated from two different collections carried out in different places of dental offices. Random amplified polymorphic DNA (RAPD) technique and similarity coefficients (calculated by Sorensen-Dice and simple matching) were applied to determine their genetic profile of randomic DNA sequences. The majority of the isolates of K. pneumoniae and K. oxytoca presented similar coefficient values (¡Ý 0.80). Thus, it was possible to identify that strain dissemination occurred mainly via the hands of the surgeon-dentists and, finally, to determine the genetic similarity of the strains from dental office environments.(AU)


Subject(s)
Animals , Klebsiella oxytoca , Genetic Profile , Klebsiella pneumoniae , Dental Offices
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