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1.
Rev. bras. plantas med ; 18(2,supl.1): 597-604, 2016. tab, graf
Article in Portuguese | LILACS | ID: biblio-830061

ABSTRACT

RESUMO Este trabalho analisa o efeito larvicida do óleo Zingiber officinale Roscoe contra larvas em terceiro estágio do mosquito Aedes aegypti. Extraiu-se quantitativamente o óleo essencial por hidrodestilação e calculou-se a CL50 do óleo, a partir dos métodos de Reed-Muench e Pizzi, respectivamente. O óleo essencial obteve CL50 de 76,07 (±2,24) μg mL-1 e rendimento de 0,52% m/v. Os resultados indicam que o óleo essencial avaliado é composto por substâncias que propiciam efeito larvicida contra Aedes aegypti.


ABSTRACT This study analyzes the larvicidal effect of the oil of Zingiber officinale Roscoe against larvae in third stage of the mosquito Aedes aegypti (Linnaeus 1792). The essential oil was extracted quantitatively by hydrodistillation and we calculated the oil’s LC50 from Reed-Muench (1938) and Pizzi (1950) methods, respectively. The essential oil obtained LC50 of 76.07 (±2.24) ug mL-1, and yield of 0.52% m/v. The results indicate that the essential oil assessed has substances that provide larvicidal effect against Aedes aegypti.


Subject(s)
Aedes/classification , Larvicides/classification , Oils, Volatile/analysis , Ginger/classification
2.
Rev. bras. plantas med ; 16(3): 505-512, jul.-set. 2014. tab
Article in Portuguese | LILACS | ID: lil-722269

ABSTRACT

Objetivou-se com este trabalho identificar, quantificar os constituintes, e avaliar a atividade antibacteriana dos óleos essenciais extraídos de rizomas de açafrão (Curcuma longa L.) e gengibre (Zingiber officinale Roscoe) cultivados nas condições de Manaus/AM frente a 14 salmonelas entéricas isoladas de frango resfriado. A extração dos óleos essenciais foi realizada utilizando-se aparelho tipo Clevenger e a composição determinada por Cromatografia Gasosa acoplada a Espectrometria de Massas (CG-MS). A atividade antibacteriana foi realizada com o emprego de técnica de microdiluição em caldo. O óleo essencial de gengibre se mostrou expressivamente mais eficiente do que o óleo de açafrão, tanto em termos de ação bacteriostática (concentração inibitória mínima de 2500 a 5000 µg.mL-1) quanto bactericida (concentração bactericida mínima de 5000 a 10000 µg.mL-1) observando-se variação apenas em duas as amostras em termos de resistência a ação bactericida deste óleo. Assim, o óleo essencial de gengibre, representa uma alternativa para o controle de Salmonella enterica, entretanto, demais estudos abordando o sinergismo com alimentos são indicados.


The objective of this work was to identify, quantify constituents and evaluate the antibacterial activity of essential oils from rhizomes of turmeric (Curcuma longa L.) and ginger (Zingiber officinale Roscoe) grown under conditions of Manaus/AM front of enteric salmonella isolated from chilled poultry. The extraction of essential oils was performed using the Clevenger type apparatus and composition determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The antibacterial activity was performed with the use of microdilution broth. The essential oil of ginger proved significantly more efficient than tumeric oil, both in terms of bacteriostatic action (minimum inhibitory concentration 2500-5000 mg µg mL-1) and bactericidal (minimum bactericidal concentration 5000-10000 mg µg mL-1) observing changes in only two samples in terms of resistance to bactericidal activity of this oil. Thus, the essential oil of ginger, is an alternative for the control of Salmonella enterica, however, other studies addressing the synergism with food are indicated.


Subject(s)
Anti-Infective Agents/analysis , Curcuma/classification , Ginger/classification , Oils, Volatile/pharmacology , Feeding Behavior , Food Preservation/classification , Plants, Medicinal/metabolism , Salmonella enterica/isolation & purification
3.
J Biosci ; 2008 Mar; 33(1): 81-90
Article in English | IMSEAR | ID: sea-111198

ABSTRACT

Soft rot is a serious disease in ginger (Zingiber of?cinale Roscoe),imposing a considerable economic loss annually in all ginger-producing countries.In this study,mRNA differential display was employed to identify genes whose expression was altered in a soft rot-resistant accession of Zingiber zerumbet (L.) Smith,a wild relative of ginger, in response to Pythium aphanidermatum (Edson) Fitzp.,which is the principal causative agent of soft-rot disease in ginger.Analysis using 68 primer combinations identified 70 differentially expressed transcript-derived fragments (TDFs),of which 34 TDFs were selected for further analysis following reverse northern screening.Cloning and sequence characterization of the 34 TDFs yielded a total of 54 distinct clones.Functional categorization of these clones revealed seven categories,of which the defence/stress/signalling group was the largest,with clones homologous to genes known to be actively involved in various pathogenesis-related functions in other plant species.The significance of these genes in relation to the resistance response in Z.zerumbet is discussed.This study has provided a pool of candidate genes for detailed molecular dissection of the defence mechanisms in Z.zerumbet and for accessing wild genetic resources for the transgenic improvement of ginger.


Subject(s)
Gene Expression Profiling , Genes, Plant , Ginger/classification , Plant Diseases/microbiology , Pythium/pathogenicity , RNA, Messenger/metabolism , Sequence Analysis, DNA , Transcription, Genetic
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