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Braz. j. oral sci ; 21: e224977, jan.-dez. 2022. ilus
Article in English | LILACS, BBO | ID: biblio-1354723


Aim: This study aims to evaluate the clinical assessment results of periimplant soft tissue with morse taper (internal abutment connection). Methods: The study was conducted using a rapid review by searching the articles from PubMed NCBI and Cochrane by using keywords. All articles were selected by the year, duplication, title, abstract, full-text, and finally, all selected articles were processed for final review. Following clinical parameters were included; Periimplant Probing Pocket Depth (PPD), Plaque Score (PS), modified Plaque Index (mPI), Mucosal Thickness (MTh), Gingival Height (GH), periimplant mucosal zenith, Pink Esthetic Score (PES), Bleeding On Probing (BOP), Sulcus Bleeding Index (SBI), and modified Gingival Index (mGI). Results: 9 selected articles were obtained from the initial literature searching count of 70 articles. The overall samples included 326 morse taper implants. Based on the evaluation, 3 out of 4 articles reported pocket depth < 4 mm, no bleeding was reported in 2 out of 4 articles. 4 out of 4 articles reported low plaque accumulation, low soft tissue recession was reported in 3 out of 3 articles, and 4 out of 4 articles reported acceptable PES values. Conclusion: The evaluations indicate that the morse taper (internal abutment connection) has favorable assessment results based on various clinical parameters

Dental Implants , Dental Abutments , Soft Tissue Injuries , Dental Implant-Abutment Design , Gingiva , Mouth Mucosa
Braz. dent. sci ; 25(2): 1-7, 2022. tab
Article in English | LILACS, BBO | ID: biblio-1368112


Objective: Impacted tooth develops when the tooth fails to erupt into its anatomical functional position. The aim of this prospective study was to find out common clinical effects of impacted lower molar on adjacent tissues and to evaluate the relationship between signs and symptoms of impacted tooth as regards gender and age respectively. Material and Methods: Data for this study were obtained using a well-structured questionnaire at the Teem Dental Hospital Warri, Nigeria. The data included age, sex, and clinical features of patients with confirmed diagnosis of impacted lower third molar following clinical examination and radiographic investigation. Data were analysed and chi-square was employed. Results: A total of 131 patients were examined, 57 (44.5%) were males and 74 (56.5%) were females within the ages of 10-40 years. Patients within the ages of 21-25 years had the highest frequency (32.1%) of impacted lower third molar. It was observed that impacted tooth had a gender predilection towards females than males. Inflamed gingivae around lower 3rd molar 60(45.8%) and pain on the lower third molar 72(55.0%) were most predominantly associated with impacted third molar teeth. No significant association was observed between age (0.909) and gender (0.461) against symptoms of impacted tooth but significant association (0.001) between age and sign was observed. Conclusion: The most commonly associated effect of impacted third molar is inflammation of the adjacent gingivae alongside pain around the lower third molar. Prevalence of impacted molar tooth was gender based with age being a predilection factor in its signs of presentation. (AU)

Objetivo: O dente impactado se desenvolve quando o dente não consegue irromper em sua posição anatômica funcional. O objetivo deste estudo prospectivo foi identificar os efeitos clínicos comuns do molar inferior impactado nos tecidos adjacentes e avaliar a relação entre os sinais e sintomas do dente impactado em relação ao sexo e idade, respectivamente. Material e Métodos: Os dados para este estudo foram obtidos por meio de um questionário bem estruturado no Teem Dental Hospital Warri, Nigéria. Os dados incluíram idade, sexo e características clínicas de pacientes com diagnóstico confirmado de terceiro molar inferior impactado após exame clínico e investigação radiográfica. Os dados foram analisados e o teste qui-quadrado foi empregado. Resultados: Foram examinados 131 pacientes, 57 (44,5%) do sexo masculino e 74 (56,5%) do sexo feminino na faixa etária de 10 a 40 anos. Pacientes com idades entre 21 a 25 anos tiveram a maior frequência (32,1%) de terceiros molares inferiores impactados. Observou-se que o dente impactado teve uma predileção de gênero para o sexo feminino em relação ao masculino. Gengiva inflamada ao redor do 3º molar inferior 60 (45,8%) e dor no terceiro molar inferior 72 (55,0%) foram predominantemente associadas a terceiros molares impactados. Não foi observada associação significativa entre idade (0,909) e sexo (0,461) diante sintomas de dente impactado, mas foi observada associação significativa (0,001) entre idade e sinal. Conclusão: O efeito mais comumente associado ao terceiro molar impactado é a inflamação da gengiva adjacente associada à dor ao redor do terceiro molar inferior. A prevalência de dente molar impactado foi baseada no gênero, sendo a idade um fator de predileção em seus sinais de apresentação.(AU)

Humans , Male , Female , Adolescent , Adult , Pain , Tooth, Impacted , Gingiva , Molar, Third
Braz. J. Pharm. Sci. (Online) ; 58: e18655, 2022. tab, graf
Article in English | LILACS | ID: biblio-1364423


Abstract Periodontitis is an oral disease associated with inflammation and pain with swollen and bleeding gums. In the present study, dental pastes containing NSAIDs, namely, diclofenac sodium and nimesulide (1 % w/w) were prepared to treat periodontitis. Dental pastes of diclofenac sodium and nimesulide (1 % w/w) were prepared with/without mucoadhesive hydrocolloid polymers such as sodium carboxy methyl cellulose (NaCMC), hydroxyl ethyl cellulose (HEC) and methyl cellulose (MC) by conventional trituration method. The pH, drug content, viscosity, tube spreadability and tube extrudability of these prepared dental pastes were measured. These dental pastes of diclofenac sodium and nimesulide (1 % w/w) were characterized by FTIR analyses for drug-excipient compatibility. The in vitro drug releases from these dental pastes in 6.4 pH phosphate buffer solution displayed sustained release over longer period and the drug release rate was found to be decreased when the concentration of mucoadhesive polymer was increased. These dental pastes displayed good adhesion to the oral mucosa revealing more retention time in mouth when tested for ex vivo mucoadhesion using bovine cheek pouch. The stability study results reveal that the DC3 and NC3 dental paste formulations were found stable enough over a longer period in different storage conditions. The present study revealed that the prepared mucoadhesive dental pastes of diclofenac sodium and nimesulide (1 % w/w) had good adhesion with the oral mucosa to maintain consistent release of drugs over prolonged time.

Toothpastes/analysis , Pharmaceutical Preparations , Anti-Inflammatory Agents, Non-Steroidal/analysis , Mouth , Mouth Mucosa/abnormalities , Periodontitis , In Vitro Techniques/methods , Diclofenac/adverse effects , Disease/classification , Spectroscopy, Fourier Transform Infrared , Drug Liberation , Gingiva/abnormalities , Inflammation/complications
Ortodoncia ; 85(170): 8-15, jul.-dic. 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1362999


Este trabajo tuvo como objetivo evaluar características de la sonrisa en pacientes con necesidad de tratamiento ortodóntico. Como objetivos específicos, se planteó estudiar el arco de la sonrisa, la línea del labio superior y los corredores bucales, que son tres componentes importantes a la hora de elaborar un plan de tratamiento adecuado para devolver al paciente una sonrisa funcional y estéticamente aceptable. La muestra quedó conformada por 157 pacientes; de cada uno de ellos se obtuvo una foto de una sonrisa voluntaria. Se encontró que el arco de la sonrisa, en el 39,5 %, presentaba un arco consonante; un 20,4 %, uno plano y el 40,1 % restante, uno no consonante. Al estudiar la línea del labio superior, se observó que, en el 8,3 %, la línea era baja; en el 62,4 %, media y en el 29,3 %, alta. En cuanto a los corredores bucales, se observó que el valor medio, en proporción a la distancia intercomisural del corredor bucal total, era de 15,89 % ± desvío estándar (5,03 %); el valor total máximo, 26,19 % y el valor mínimo, 2,74 %. Conociendo la prevalencia de cada uno de estos datos, se podrá arribar al diagnóstico y posterior plan de tratamiento para cada paciente de manera individualizada(AU)

This work aimed at evaluating smile characteristics in patients in need of orthodontic treatment. As specific objectives, it was raised the issue of studying the smile arch, upper lip line and buccal corridors, which are three important components when preparing an adequate treatment plan to restore the patient a functional and aesthetically acceptable smile. The sample consisted of 157 patients and a photo of a voluntary smile was obtained from each of them. It was found that the smile arch had a consonant arch in 39.5%, 20.4% a flat arch and the remaining 40.1% a nonconsonant arch. When studying the upper lip line, it was observed that in 8.3% the line was low, 62.4% medium and 29.3% high. As regards the buccal corridors, it was observed that the mean value in proportion to the intercommissural distance of the total buccal corridor was 15.89% ± standard deviation (5.03%); maximum total value was 26.19% and minimum value 2.74%. Knowing about the prevalence of each of these data, it will be able to arrive at the diagnosis and subsequent treatment plan for each patient individually(AU)

Smiling , Photography, Dental , Esthetics, Dental , Face , Gingiva , Lip , Therapeutics
Braz. j. oral sci ; 20: e211236, jan.-dez. 2021. ilus
Article in English | LILACS, BBO | ID: biblio-1253730


Aim: Dental imaging has been widely used for diagnosis in dentistry. However, dental X-ray may induce cytotoxicity leading to apoptosis in oral mucosa cells. The present study aimed to observe the maturation pattern of buccal and gingival cells after exposure to X-ray radiation from analog/digital panoramic scanning and cone beam computed tomography (CBCT). Methods: The research samples were 40 subjects who fulfilled the inclusion and exclusion criteria. The subjects were divided into the exposed (patients who received analog/digital panoramic radiography or CBCT) and controlled (patients who had no radiography examinations) groups, with 10 subjects in each group. Exfoliative cytology smears were obtained from buccal mucosa and gingiva before exposure (or on day 0 for the control group) and 10 days later. The cells were stained with the Papanicolaou method. Then, the superficial, intermediate, and parabasal cells were counted in each glass slide. Results: No significant differences (p > 0.05) were observed among all cell types between day 0 and 10 in the control group. Meanwhile, after exposure to three kinds of radiography examinations, the frequency of intermediate cells in buccal mucosa and gingiva increased (p < 0.05), but that of superficial cells decreased (p < 0.05) significantly. No significant difference was found in the parabasal cells (p > 0.05). The frequency differences between intermediate and superficial cells showed no significant difference between the buccal mucosa and gingiva. Conclusion: Analog/digital panoramic radiography and CBCT exposure can induce cytotoxicity by altering the maturation pattern of buccal mucosa cells and gingiva, so it is strongly recommended to only perform these procedures if necessary and avoid repeated exposure to the same patient

Humans , Radiography, Panoramic , Cone-Beam Computed Tomography , Papanicolaou Test , Gingiva , Mouth Mucosa
Article in English | WPRIM | ID: wpr-878449


OBJECTIVES@#The proliferation, migration capacity, and expression of activation-related proteins of NHGFs+Cal27-exo were determined by coculturing Cal27 exosome (Cal27-exo) with normal human gingival fibroblasts (NHGFs) to explore the effects of Cal27-exo on the activation and biological behavior of NHGFs.@*METHODS@#Cal27-exo was extracted using supercentrifugation, and exosomes were identified using Western blot, transmission electron microscopy (TEM), and particle size detection. Cal27-exo was cocultured with NHGFs to detect the uptake of Cal27-exo by NHGFs, and the proliferation and migration capacity of NHGFs+Cal27-exo were detected using CCK8 and wound healing tests, respectively. The expression levels of NHGF activation-related proteins, i.e., matrix metalloproteinase-9 (MMP-9), fibroblast-activating protein (FAP), alpha smooth muscle actin (αSMA), and transforming growth factor-β (TGF-β), were detected using real-time quantitative polymerase chain reaction (qRT-PCR).@*RESULTS@#Cal27-exo was extracted u-sing supercentrifugation, and Western blot showed the positive expression levels of Alix and CD63. TEM showed that Cal27-exo had a circular double-layer vesicle. The particle size was between 30 and 150 nm. Cal27-exo labeled with PKH67 entered NHGFs after the coculture method. The wound healing test showed that the migration capacity of NHGFs+Cal27-exo was stronger after the scratch compared with that of NHGFs. CCK8 results showed that the proliferation activity of NHGFs+Cal27-exo was enhanced. qRT-PCR results showed that the MMP-9 levels of NHGFs+Cal27-exo were upregulated, whereas the TGF-β and αSMA mRNA levels of NHGFs+Cal27-exo were downregulated (@*CONCLUSIONS@#The proliferation and migration ability of NHGFs+Cal27-exo are enhanced, and the mRNA expression of related proteins is changed. Cal27-exo can activate NHGFs, which suggests that Cal27-exo has potential significance in tumor invasion and metastasis.

Cell Proliferation , Exosomes , Fibroblasts , Gingiva , Humans , Matrix Metalloproteinase 9
Article in Chinese | WPRIM | ID: wpr-878424


OBJECTIVES@#This study was performed to clarify the effects of sitagliptin on @*METHODS@#Healthy gingival samples were collected from the donors. HGFs were isolated with enzymic digestion method and identified. The effects of LPS and sitagliptin on cell viability were detected by cell-counting kit-8 (CCK8). The mRNA levels of inflammatory cytokines, namely, interleukin (IL)-6, IL-8, C-C motif ligand 2 (CCL2), and superoxide dismutase 2 (SOD2), were evaluated by quantity real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immune sorbent assay (ELISA) was used to measure the secretion protein levels of IL-6, IL-8, and CCL2. Western blot analysis was used to further investigate the activation of nuclear factor (NF)-κB signaling pathway. The effect of NF-κB pathway inhibitor BAY11-7082 on LPS-induced HGF inflammatory cytokines at the gene level was verified by qRT-PCR.@*RESULTS@#Low concentrations of sitagliptin (0.1, 0.25, and 0.5 µmol·L@*CONCLUSIONS@#Sitagliptin could significantly inhibit LPS-induced HGF inflammatory response by blocking the NF-κB signaling pathway activation.

Fibroblasts , Gingiva/metabolism , Humans , Lipopolysaccharides , NF-kappa B/metabolism , Signal Transduction , Sitagliptin Phosphate
Rev. Fundac. Juan Jose Carraro ; 24(44): 48-53, 2021. ilus, tab, graf
Article in Spanish | LILACS | ID: biblio-1223615


La odontología estética no es una disciplina especial o área de la odontología en sí misma, pero con consideraciones funcionales y biológicas, representa uno de los objetivos de las intervenciones de tratamiento dental, que abarca todas las áreas de especialidad, desde la odontología preventiva y restaurativa hasta la prostodoncia, ortodoncia, periodoncia, como, así como la cirugía oral y maxilofacial. El cénit gingival es un importante componente de la sonrisa y su estudio sigue siendo muy relevante en la odontoestética internacional. En el presente trabajo se determinaron distancias del cenit al eje longitudinal y se correlacionaron con otros parámetros gingivales, se establecieron diferencias en las alturas de las papilas interdentales y se correlacionaron los datos métricos de las piezas dentarias anteriores de la población estudiada y los datos conocidos con el fin de obtener datos estadísticos relevantes. Los datos mesurables fueron obtenidos de pacientes de ambos sexos, (18 ­ 25) años, con piezas dentarias del grupo anterior y superior a saber: incisivos centrales, incisivos laterales y caninos superiores, normalmente implantados, libres de lesiones o restaruración, ausencia de enfermedad gingivoperiodontal y sin tratamientos ortodoncicos. El trabajo observacional, descriptivo y tranversal arrojó resultados basados en la estadística preponderante. Provee dimensiones y proporciones de dientes maxilares que pueden adaptarse a pacientes individuales en relación con parámetros establecidos en la odontoestética. Estos datos pueden ser pautas útiles para el diagnóstico y la planificación del tratamiento (especialmente cirugía periodontal) en la dentición maxilar (AU)

A esthetic dentistry is not a special discipline or area of dentistry itself, but with functional and biological considerations, it represents one of the objectives of dental treatment interventions, which covers all areas of specialty, from preventive and restorative dentistry up to prosthodontics, orthodontics, periodontics, as well as oral and maxillofacial surgery. The gingival cenith is an important component of the smile and its study remains very relevant in international dentistry. In this work, distances from the cenith to the longitudinal axis were determined and correlated with other gingival parameters, differences in the heights of the interdental papillae were established and the metric data of the anterior teeth of the studied population were correlated and the known data with in order to obtain relevant statistical data. The measurable data were obtained from patients of both sexes, (18 - 25) years, with teeth of the anterior and superior group, namely: central incisors, lateral incisors and upper canines, normally implanted, free of lesions or restoration, absence of disease gingivoperiodontal and without orthodontic treatments. The observational, descriptive and transverse work produced results based on the preponderant statistics. It provides dimensions and proportions of maxillary teeth that can be adapted to individual patients in relation to parameters established in odontoesthetics. These data can be useful guidelines for diagnosis and treatment planning (especially periodontal surgery) in the maxillary dentition (AU)

Humans , Male , Female , Adolescent , Adult , Smiling , Esthetics, Dental , Gingiva/anatomy & histology , Periodontium/anatomy & histology , Epidemiology, Descriptive , Cross-Sectional Studies , Statistical Analysis , Cuspid , Dental Papilla/anatomy & histology , Observational Study , Incisor
Rev. cuba. med. mil ; 49(2): e498, abr.-jun. 2020. tab, fig
Article in Spanish | LILACS, CUMED | ID: biblio-1138993


Introducción: La gingivoestomatitis estreptocócica es una inflamación aguda de las encías y mucosa bucal. Es una infección específica por estreptococo, poco frecuente. Objetivo: Valorar la correspondencia entre el diagnóstico presuntivo de la gingivoestomatitis estreptocócica, el diagnóstico etiológico y la evolución clínica de los pacientes tratados. Métodos: Se realizó un estudio descriptivo en el período comprendido de septiembre del año 2018 a abril del 2019, en el Hospital Militar "Manuel Fajardo". La población de estudio estuvo constituida por 13 pacientes de 15 a 24 años que acudieron a los servicios de urgencias estomatológicas, quienes fueron diagnosticados con gingivoestomatitis estreptocócica. Las variables estudiadas fueron, edad, sexo, características clínicas, síntomas, correspondencia entre el diagnóstico presuntivo, el etiológico y evolución de la enfermedad. Resultados: El 61,5 por ciento de los pacientes fueron del sexo masculino y la media de edad fue de 18,5 años, el sangramiento estuvo presente en la totalidad de los pacientes, el 92,3 por ciento presento úlceras, en el 76,9 por ciento la enfermedad estaba generalizada, entre los síntomas predominaron el dolor y la fiebre. El diagnóstico etiológico del estreptococo beta hemolítico fue del 84,6 por ciento. La evolución de la enfermedad con el tratamiento recibido fue satisfactoria en el 76,9 por ciento de los pacientes. Conclusiones: Se observó alta correspondencia entre el diagnóstico presuntivo realizado en la consulta de urgencias y el diagnóstico etiológico de la gingivoestomatitis estreptocócica. La mayoría de los pacientes tuvo una evolución satisfactoria con el tratamiento(AU)

Introduction: Streptococcal gingivostomatitis is an acute inflammation of the gums and oral mucosa. It is a specific streptococcus infection with low frequency. Objective: To assess the correspondence between the presumptive diagnosis of streptococcal gingivostomatitis, the etiological diagnosis and the clinical evolution of the treated patients. Methods: A descriptive study was carried out in the period from September 2018 to April 2019, at the Hospital Militar "Manuel Fajardo Rivero". The study population consisted of 13 patients aged 15 to 24 who attended the stomatological emergency services who were diagnosed with streptococcal gingivostomatitis. The variables studied were, age, sex, clinical characteristics, symptoms, correspondence between presumptive diagnosis, etiological and disease evolution. Results: 61.5 percent of the patients were male and the average age was 18.5 years, bleeding was present in all patients, 92.3 percent had ulcers, in 76.9 percent the disease was widespread, pain and fever predominated among the symptoms. The etiological diagnosis of beta hemolytic streptococcus was 84.6 percent. The evolution of the disease with the treatment received was satisfactory in 76.9 percent of the patients. Conclusions: High correspondence was observed between the presumptive diagnosis made in the emergency department and the etiological diagnosis of streptococcal gingivostomatitis. The majority of the patients had a satisfactory evolution with the treatment(AU)

Humans , Male , Female , Adolescent , Adult , Gingiva , Hemorrhage , Infections , Methods , Mouth Mucosa , Epidemiology, Descriptive
Int. j. morphol ; 38(3): 683-688, June 2020. graf
Article in English | LILACS | ID: biblio-1098307


The aim was to evaluate bone repair and gingival tissue repair in osteopenic rats. Fifteen female wistar rats were included; in all of them ovariectomy was realized to induce osteopenia; after 45 days, the animals were submitted to 2 surgical techinques 1) dental extraction of the upper central incisor with no socket preservation and 2) 5 mm cranial defect in the calvarium; 5 rats were included in the control group (G1) withput alendronate application; in the group 2 (G2) was used subcutenous alendronate (0.5 mg/kg) once for three weeks and then was realizd the both surgical techniques. In group 3 (G3), after ovariectomy was realized the both dental extraction and the calvarium defect and after that was realized the alendronate protocol. In each group, after six week was realized euthanasia and descriptive histological analysis of the surgical areas involved. In bone formation of the 5 mm cranial defect was observed with good progression in the 3 experimental models and no modification in quality of bone repair was observed. For the gingival tissue in the extraction socket, no differences were observed between G1 and G3. On other hand, in G2 a thinner and reduced gingival epithelium was found. Our results showed that alendronate was not an obstacle for bone repair; deficiencies in re-epithelialization of oral mucosa show the impact of alendronate before dental extraction.

El objetivo fue evaluar la reparación ósea y gingival en ratas con osteopenia. Quince ratas wistar hembras fueron incluidas; en todas ellas se realizo ovarectomia y fue realizada la inducción de osteopenia; después de 45 días, los animales fueron sometidos a dos técnicas quirúrgicas 1) extracciones dentales del incisivo central superior sin preservación alveolar y 2) creación de un defecto craneano de 5 mm en la calota; 5 animales fueron incluidos como grupo control (G1) sin la aplicación de alendronato; en el grupo 2 (G2) se utilizó alendronato subcutáneo (0,5 mg/kg) una vez a la semana durante 3 semanas. En el grupo 3 (G3), después de la ovarectomia se realizó la exodoncia y el defecto en el cráneo y después de ello se inicio el protocolo con alendronato. En cada grupo, después de seis semanas se realizó la eutanasia con descripción histológica de los hallazgos. En el hueso formado en el defecto craneano de 5 mm se observó una adecuada progresión de reparación en los 3 modelos experimentales y no se observó cambios importantes en el modelo de reparación. Para el tejido gingival en el sitio de extracción, no se observaron diferencias entre el grupo G1 y G3. Por otra parte, el G2 presentó un tejido mas delgado con reducción del epitelio gingival; nuestros resultados demuestran que el alendronato no fue un obstáculo en la reparación ósea; deficiencias en la re epitelización de la mucosa oral muestran el impacto del alendronato después de la exodoncia.

Animals , Female , Rats , Bone Diseases, Metabolic/drug therapy , Bone Regeneration/drug effects , Alendronate/administration & dosage , Gingiva/drug effects , Osteonecrosis/drug therapy , Osteoporosis/drug therapy , Bone Diseases, Metabolic/complications , Ovariectomy , Rats, Wistar , Diphosphonates/administration & dosage
Dental press j. orthod. (Impr.) ; 25(2): 44-51, Mar.-Apr. 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1133656


ABSTRACT Objective: To evaluate facial profile changes promoted by polymethyl methacrylate (PMMA) cement graft to reduce excessive gingival display due to hyperactivity of the elevator muscles of the upper lip during smiling. Methods: Eleven patients (all females, age range: 20 to 43 years) presenting gingival smile that were treated with PMMA cement grafts in a private clinic were selected for this retrospective study. Three angular and ten linear cephalometric facial profile measurements were performed preoperatively (baseline, T1) and at least 6 months postoperatively (T2). Differences between T1 and T2 were verified by Wilcoxon test, and the correlation between the thickness of the graft and facial profile changes was statistically evaluated by Spearman's Coefficient test. The significance level was set at p< 0.05. Results: The nasolabial angle (p= 0.03) and the labial component of the nasolabial angle showed statistically significant differences (p= 0.04), with higher values in T2. No correlations were found between the graft thickness and the statistically significant facial profile changes (p> 0.05). Conclusions: The PMMA bone cement graft projected the upper lip forward, thereby increasing the nasolabial angle without affecting the nasal component. No correlations between the graft thickness and the facial profile changes were detected.

RESUMO Objetivo: Avaliar as alterações do perfil facial promovidas pelo enxerto de cimento de polimetilmetacrilato (PMMA) para redução da exposição gengival excessiva devida à hiperatividade dos músculos elevadores do lábio superior durante o sorriso. Métodos: Onze pacientes (todos do sexo feminino, faixa etária de 20 a 43 anos) com sorriso gengival, tratados com enxerto de cimento de PMMA em clínica privada, foram selecionados para este estudo retrospectivo. Três medidas cefalométricas angulares e dez lineares do perfil facial foram realizadas no pré-operatório (T1) e com pelo menos seis meses de pós-operatório (T2). As diferenças entre T1 e T2 foram verificadas pelo teste de Wilcoxon, e a correlação entre a espessura do enxerto e as alterações do perfil facial foi avaliada estatisticamente pelo Coeficiente de Spearman. O nível de significância foi estabelecido em p< 0,05. Resultados: o ângulo nasolabial (p= 0,03) e o componente labial do ângulo nasolabial apresentaram diferenças estatisticamente significativas (p= 0,04), com maiores valores em T2. Não foram encontradas correlações estatisticamente significativas (p> 0,05) entre a espessura do enxerto e as alterações do perfil facial. Conclusões: O enxerto de cimento ósseo de PMMA projetou discretamente o lábio superior, aumentando o ângulo nasolabial sem afetar o componente nasal. Não foram detectadas correlações entre a espessura do enxerto e as alterações do perfil facial.

Humans , Female , Adult , Young Adult , Smiling , Bone Cements , Cephalometry , Retrospective Studies , Gingiva , Lip
Article in Chinese | WPRIM | ID: wpr-781336


Hereditary gingival fibromatosis (HGF) is a familial hereditary disease; while it is rare and usually benign, it is also characterized by the slow and progressive development of gingival tissue. This paper reports on the clinical examina-tion and history of HGF in a family of patients.

Fibromatosis, Gingival , Gingiva , Humans
Article in Chinese | WPRIM | ID: wpr-827534


As an aggressive subtype of squamous cell carcinoma, basaloid squamous cell carcinoma (BSCC) rarely occurs in the oral and maxillofacial region. The gingiva is an unusual site of BSCC. This study reported a 78-year-old male who presented with left maxillary pain. Clinical examination revealed a gingival mass in the left maxilla. Under microscope, the lesion showed typical comedo necrosis and peripheral palisading. Areas of glandular-like structures were also observed. Immunohistochemistry results revealed that the Ki-67 score of BSCC in this case was 28%, and S-100 was positive in some areas. However, P16 and CK7 were negative. Finally, a diagnosis of BSCC was made based on the pathological and immunohistochemical characteristics. The patient underwent subtotal maxillectomy. After 12 months later, the patient was alive with no evidence of disease. Combined with relevant literature, this article analyzed the clinicopathological features, differential diagnosis, diagnosis, treatment, and prognosis of BSCC. Although surgery remains the main treatment in the head and neck region, radiation-chemotherapy should be considered in some human papilloma virus-positive cases.

Aged , Carcinoma, Squamous Cell , Gingiva , Humans , Immunohistochemistry , Male , Prognosis
Article in Chinese | WPRIM | ID: wpr-827524


OBJECTIVE@#To explore the correlation among gingival thickness (GT), underlying alveolar bone thickness (BT), and other periodontal biotype characteristics in the maxillary anterior.@*METHODS@#A total of 40 young volunteers with healthy periodontal were involved in this research. The periodontal probe was previously used to divide the gingiva from thick to thin. Two records were measured by cone beam CT (CBCT) GT, which was measured at the cement-enamel junction level; and BT, which was measured at 3 locations: 1, 3, 5 mm below the alveolar crest. Oral and gypsum measurements were used to analyze the associations of the crown width/crown length ratio (CW/CL), the keratinized mucosa width (KM), and the free gingival margin curvature.@*RESULTS@#Significant difference in the GT was observed between the thick and thin biotypes, which were divided by periodontal probe (P<0.01). Difference was observed in each periodontal biotype characteristic between the thick (GT≥1 mm) and thin biotypes (GT<1 mm) (P<0.05). BT was positively associated with GT (r=0.293, P=0.001), CW/CL (r=0.273, P=0.003), KM (r=0.291, P=0.001), and free gingival margin curvature (r=0.290, P=0.001).@*CONCLUSIONS@#The transparency of the probing in the sulcus could analyze the GT qualitatively. The thick and thin biotypes have different periodontal biotype characteristics. Compared with individuals with thick biotype, those with thin biotype are susceptible to risk dental aesthetic.

Alveolar Process , Cone-Beam Computed Tomography , Gingiva , Humans , Maxilla , Tooth Crown
Braz. oral res. (Online) ; 34: e013, 2020. graf
Article in English | LILACS | ID: biblio-1089379


Abstract This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated with LPS 10 μg/ml and treated with 15d-PGJ2 1 and 2 μg/ml, and ScLL 2 and 5 μg/ml, for 1 and 3h, and then evaluated for viability by MTT assay. Supernatant was collected to detect IL-6 and TGFβ-1 release, by ELISA. Positive control was cells kept in Dulbecco's Modified Eagle's Medium, and negative control was those kept in LPS. Data were analyzed by ANOVA and Dunnett's test (α = 0.05). No significant difference was found in viability among experimental groups at 1h (p > 0.05). Percentage of ScLL 5 µg/ml viable cells was similar to that of positive control at evaluated periods (p > 0.05), whereas the other groups had lower levels than the positive control (p < 0.05). IL-6 release was statistically higher for ScLL 5 μg/ml and 15d-PGJ2 2 µg/ml at 1h, compared with the other treated groups and positive control (p < 0.05). No significant differences were found among the groups at 3h (p > 0.05), except for ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml, which showed lower IL-6 release compared with that of negative control (p < 0.05). No significant difference was found among the groups for TGFβ-1 release (p > 0.05). Results indicated that ScLL 5 μg/ml did not interfere in viability, and ScLL 2 µg/ml and 15d-PGJ2 1 µg/ml demonstrated reduced IL-6 release. Tested substances had no effect on TGFβ-1 release.

Humans , Prostaglandin D2/analogs & derivatives , Lipopolysaccharides/pharmacology , Interleukin-6/metabolism , Plant Lectins/pharmacology , Transforming Growth Factor beta1/metabolism , Fibroblasts/drug effects , Fibroblasts/metabolism , Reference Values , Time Factors , Enzyme-Linked Immunosorbent Assay , Prostaglandin D2/pharmacology , Cell Survival/drug effects , Cells, Cultured , Analysis of Variance , Statistics, Nonparametric , Transforming Growth Factor beta1/drug effects , Gingiva/cytology
Braz. oral res. (Online) ; 34: e012, 2020. graf
Article in English | LILACS | ID: biblio-1055530


Abstract Lipoproteins are important bacterial immunostimulating molecules capable of inducing receptor activator of nuclear factor-κB (RANKL) and osteoclast formation in vitro and in vivo . Although these molecules are present in periodontopathogenic bacteria, their role in periodontitis is not known. In this study, we used Pam2CSK4 (PAM2), a synthetic molecule that mimics bacterial lipoprotein, to investigate the effects of lipoproteins on periodontitis in mice. C57BL/6 male mice were randomly divided into three experimental groups: 1) Negative control group: animals received vehicle injection; 2) Positive control group: animals received injection of Escherichia coli lipopolysaccharide (LPS); 3) PAM2 group: animals received PAM2 injection. All the injections were performed bilaterally every other day into the palatal mucosa between first and second molars. After twenty-four days, the animals were euthanized to assess alveolar bone volume (micro-CT), cellular and extracellular composition in the gingiva (stereometric analysis), and osteoclast numbers (TRAP staining). Treatment with either PAM2 or LPS induced gingival inflammation, as demonstrated by increased infiltration of inflammatory cells and enhanced angiogenesis, associated with a smaller number of fibroblasts and decreased extracellular matrix. Importantly, treatment not only with LPS but also with PAM2 resulted in a larger number of TRAP+ multinucleated osteoclasts and significant loss of alveolar bone. Collectively, our data demonstrate that PAM2 can induce gingival inflammation and bone loss in mice, broadening the avenues of investigation into the role of lipoproteins in the pathogenesis of periodontal disease.

Animals , Male , Periodontitis/etiology , Periodontitis/pathology , Toll-Like Receptor 2/antagonists & inhibitors , Lipopeptides/pharmacology , Osteoclasts/drug effects , Periodontitis/microbiology , Time Factors , Random Allocation , Alveolar Bone Loss/etiology , Alveolar Bone Loss/pathology , Disease Models, Animal , X-Ray Microtomography , Alveolar Process/drug effects , Alveolar Process/pathology , Tartrate-Resistant Acid Phosphatase , Gingiva/drug effects , Gingiva/pathology , Gingivitis/etiology , Gingivitis/pathology , Mice, Inbred C57BL
Braz. oral res. (Online) ; 34: e038, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1100932


Abstract The possible role of B-cell growth and differentiation-related cytokines on the pathogenesis of diabetes-related periodontitis has not been addressed so far. The aim of this study was to evaluate the effects of diabetes mellitus (DM) on the gene expression of proliferation-inducing ligand (APRIL) and B-lymphocyte stimulator (BLyS), two major cytokines associated to survival, differentiation and maturation of B cells in biopsies from gingival tissue with periodontitis. Gingival biopsies were obtained from subjects with periodontitis (n = 17), with periodontitis and DM (n = 19) as well as from periodontally and systemically healthy controls (n = 10). Gene expressions for APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were evaluated using qPCR. The expressions APRIL, BLyS, RANKL, OPG, TRAP and DC-STAMP were all higher in both periodontitis groups when compared to the control group (p < 0.05). Furthermore, the expressions of BLyS, TRAP and RANKL were significantly higher in the subjects with periodontitis and DM when compared to those with periodontitis alone (p < 0.05). The mRNA levels of BLyS correlated positively with RANKL in the subjects with periodontitis and DM (p < 0.05). BLyS is overexpressed in periodontitis tissues of subjects with type 2 DM, suggesting a possible role of this cytokine on the pathogenesis DM-related periodontitis.

Humans , Male , Female , Adult , Aged , Periodontitis/immunology , Periodontitis/pathology , Diabetes Mellitus, Type 2/complications , B-Cell Activating Factor/analysis , Osteogenesis/immunology , Reference Values , Biopsy , RNA, Messenger/analysis , Biomarkers/analysis , Case-Control Studies , Gene Expression , Cytokines/analysis , Cytokines/physiology , Statistics, Nonparametric , Diabetes Mellitus, Type 2/immunology , Tumor Necrosis Factor Ligand Superfamily Member 13/analysis , Real-Time Polymerase Chain Reaction , Gingiva/immunology , Gingiva/pathology , Middle Aged
J. appl. oral sci ; 28: e20190145, 2020. graf
Article in English | LILACS | ID: biblio-1056576


Abstract Ultrasonic wave technology is widely used during dental treatments. We previously demonstrated that this method protects the gingival tissue. However, the physiological change on the gingival microvasculature caused by this method remains unclear. Objective The aim of this study was to investigate the relationship between the morphological and physiological effects on gingival microcirculation when preparing teeth, using the conventional dental turbine or ultrasonic method. Methodology The lower premolar teeth of beagle dogs were prepared along the gingival margin by using a dental turbine or ultrasonic wave instrument. Gingival vasculature changes were investigated using scanning electron microscopy for corrosion resin casts. Gingival blood flow at the preparation site was determined simultaneously by laser Doppler flowmetry. These assessments were performed immediately (Day 0), at 7 days and 30 days after tooth preparation. Results At day 0, in the turbine group, blood vessels were destroyed and some resin leaked. Furthermore, gingival blood flow at the site was significantly increased. In contrast, the ultrasonic group demonstrated nearly normal vasculature and gingival blood flow similar to the non-prepared group for 30 days after preparation. No significant alterations occurred in gingival circulation 30 days after either preparation; however, the turbine group revealed obvious morphological changes. Conclusions Based on multiple approach analyses, this study demonstrated that ultrasonic waves are useful for microvascular protection in tooth preparation. Compared with a dental turbine, ultrasonic wave instruments caused minimal damage to gingival microcirculation. Tooth preparation using ultrasonic wave instruments could be valuable for protecting periodontal tissue.

Humans , Animals , Female , Dogs , Tooth Preparation/instrumentation , Ultrasonic Waves , Gingiva/blood supply , Microcirculation/physiology , Time Factors , Microscopy, Electron, Scanning , Clinical Protocols , Reproducibility of Results , Laser-Doppler Flowmetry/methods , Tooth Preparation/methods , Dental Instruments
Rev. Fac. Odontol. (B.Aires) ; 35(81): 41-50, 2020. ilus
Article in Spanish | LILACS | ID: biblio-1179866


Las complicaciones del tejido blando periimplantar condicionan la apariencia estética y el pronóstico clínico de los implantes y son, en la actualidad, cada vez más diagnosticadas. Los defectos gingivales asociados a implantes dentales incluyen recesiones, fenestraciones o dehiscencias en la superficie mucosa vestibular, inflamación gingival, ausencia de encía insertada/queratinizada, falta de volumen y presencia de concavidades gingivales que generan sombras y oscuridad en la mucosa. La detección de éstas en forma temprana permite establecer un plan de tratamiento en busca de soluciones eficaces. Mediante la presentación de una serie de casos, abordaremos distintos procedimientos para aumento de los tejidos blandos periimplantarios y la corrección de defectos. La ganancia de encía queratinizada ha demostrado tener un impacto positivo en la estabilidad a largo plazo de todos los tejidos implantarios (AU)

Humans , Dental Implantation, Endosseous , Esthetics, Dental , Gingiva/transplantation , Gingival Diseases , Mucous Membrane , Surgical Flaps , Tooth Extraction , Oral Surgical Procedures