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1.
J. appl. oral sci ; 28: e20190266, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1056586

ABSTRACT

Abstract Objective: The microbial composition of pericoronitis (Pc) is still controversial; it is not yet clear if the microbial profile of these lesions is similar to the profile observed in periodontitis (Pd). Therefore, the aim of the present study was to describe the microbial profile of Pc lesions and compare it directly with that of subjects with Pd. Methodology: Subjects with Pc and Pd were selected, and subgingival biofilm samples were collected from (i) third molars with symptomatic Pc (Pc-T), (ii) contralateral third molars without Pc (Pc-C) and (iii) teeth with a probing depth >3 mm from subjects with Pd. Counts and proportions of 40 bacterial species were evaluated using a checkerboard DNA-DNA hybridization technique. Results: Twenty-six patients with Pc and 18 with Pd were included in the study. In general, higher levels of microorganisms were observed in Pd. Only Actinomyces oris and Eubacterium nodatum were present in higher mean counts in the Pc-T group in comparison with the Pc-C and Pd-C groups (p<0.05). The microbiota associated with Pc-T was similar to that found in Pc-C. Sites with Pc lesions had lower proportions of red complex in comparison with the Pd sites. Conclusion: The microbiota of Pc is very diverse, but these lesions harbour lower levels of periodontal pathogens than Pd.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Pericoronitis/microbiology , Periodontitis/microbiology , Bacteria/isolation & purification , Reference Values , Activation Analysis , DNA Probes , Cross-Sectional Studies , Biofilms , Bacterial Load , Gingiva/microbiology
2.
Pesqui. vet. bras ; 39(7): 454-459, July 2019. tab
Article in English | LILACS, VETINDEX | ID: biblio-1040710

ABSTRACT

Periodontitis is an inflammatory response in a susceptible host caused by complex microbiota, predominantly composed of Gram-negative anaerobic bacteria. Aiming to characterize the subgingival bacterial microbiota associated with ovine periodontitis, polymerase chain reaction (PCR) was performed in subgingival periodontal pocket samples of 14 sheep with severe periodontitis and in subgingival sulcus biofilm of 14 periodontally healthy sheep in search mainly of Gram-negative and Gram-positive microorganisms considered important periodontopathogens. The most prevalent bacteria in the sheep with periodontal lesions were Tannerella forsythia (78.6%), Treponema denticola (78.6%), Fusobacterium nucleatum (64.3%), and Porphyromonas gingivalis (50%), whereas in the healthy sheep, F. nucleatum (42.8%) was the most often detected bacterium. Statistically significant differences were observed for Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia, and T. denticola (p<0.05) in the sheep with periodontitis in the comparison between groups. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis, and Porphyromonas gulae were not detected in any of the samples analyzed. In conclusion, C. rectus, E. faecium, P. nigrescens, T. forsythia, and T. denticola were associated with severe lesions caused by ovine periodontitis, and F. nucleatum was the most prevalent microorganism in the subgengival sulcus biofilm of healthy sheep.(AU)


Periodontite é a resposta inflamatória de um hospedeiro suscetível causada por complexa microbiota, composta predominantemente por bactérias anaeróbias Gram-negativas. Com o objetivo de caracterizar a microbiota bacteriana subgengival associada à periodontite ovina foi realizada a reação em cadeia da polimerase (PCR) de amostras de biofilme subgengival de 14 ovinos com a enfermidade e 14 ovinos periodontalmente saudáveis, com destaque para micro-organismos Gram-negativos e Gram-positivos considerados importantes periodontopatógenos. As bactérias mais prevalentes em 14 animais com lesões periodontais foram Tannerella forsythia (78,6%), Treponema denticola (78,6%), Fusobacterium nucleatum (64,3%) e Porphyromonas gingivalis (50%). Entretanto, nos 14 ovinos sem lesões periodontais, F. nucleatum (42,8%) foi a bactéria mais detectada. Associação estatisticamente diferente foi observada para Campylobacter rectus, Enterococcus faecium, Prevotella nigrescens, T. forsythia e T. denticola (p<0,05) nos ovinos com periodontite em comparação entre os dois grupos. Aggregatibacter actinomycetemcomitans, Enterococcus faecalis e Porphyromonas gulae não foram detectados em nenhuma das amostras pesquisadas. Conclui-se que C. rectus, E. faecium, P. nigrescens, T. forsythia e T. denticola estão associados às lesões resultantes da periodontite ovina com manifestação clínica grave e F. nucleatum o micro-organismo mais prevalente no biofilme subgengival de animais periodontalmente sadios.(AU)


Subject(s)
Animals , Periodontal Diseases/veterinary , Periodontitis/veterinary , Sheep , Gingiva/microbiology , Bacteria, Anaerobic , Polymerase Chain Reaction/veterinary , Microbiota
3.
Braz. oral res. (Online) ; 33(supl.1): e064, 2019. tab, graf
Article in English | LILACS | ID: biblio-1039323

ABSTRACT

Abstract The aim was of this study was to determine the current weight of evidence for the existence of specific differences between the microbiota of healthy teeth and healthy implants, or of teeth with periodontitis and implants with peri-implantitis. A systematic review was conducted according to the PRISMA statement. The MEDLINE, EMBASE and Cochrane databases were searched up to February 2018 for studies comparing microbiological data of biofilm samples collected from healthy teeth and implants or from teeth with periodontitis and implants with peri-implantitis. The weight of evidence was defined in three categories (strong, moderate and mild/some), according to the difference in number of studies showing statistically significantly higher counts and/or proportions and/or abundance and/or prevalence of microorganisms in health or in disease. Of the 132 articles identified, 8 were included. A wide range of microorganisms were present in different conditions but no microorganisms showed strong, moderate or mild/some evidence for a specific association with either teeth or implants. The results of this systematic review indicated that there is insufficient evidence in the literature to support specific differences between microorganisms colonizing teeth and implants, either in health or in disease.


Subject(s)
Humans , Periodontitis/microbiology , Dental Implants/microbiology , Peri-Implantitis/microbiology , Gingiva/microbiology , Bacteria/isolation & purification , Case-Control Studies , Biofilms/growth & development , Dental Plaque/microbiology , Microbiota
4.
J. appl. oral sci ; 25(1): 82-89, Jan.-Feb. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-841158

ABSTRACT

Abstract Objective This study evaluated the influence of glycemic control on the levels and frequency of subgingival periodontal pathogens in patients with type 2 diabetes mellitus (DM) and generalized chronic periodontitis (ChP). Material and Methods Fifty-six patients with generalized ChP and type 2 DM were assigned according to the levels of glycated hemoglobin (HbA1c) into one of the following groups: HbA1c<8% (n=28) or HbA1c≥8% (n=28). Three subgingival biofilm samples from sites with probing depth (PD)<5 mm and three samples from sites with PD≥5 mm were analyzed by quantitative Polymerase Chain Reaction (PCR) for the presence and levels of Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Eubacterium nodatum, Parvimona micra, Fusobacterium nucleatum ssp. and Prevotella intermedia. Results The mean counts of F. nucleatum ssp. were statistically significantly higher in the sites with PD≥5 mm of the HbA1c≥8% group (p<0.05). Frequencies of detection of T. forsythia, E. nodatum, P. micra and F. nucleatum ssp. were all higher in the sites with PD≥5 mm of the patients with HbA1c≥8%, compared with those of patients with HbA1c<8% (p<0.05). Frequency of detection of P. intermedia was higher in the sites with PD<5 mm of the patients with HbA1c≥8% than those of the patients with HbA1c<8% (p<0.05). Conclusions Poor glycemic control, as indicated by HbA1c≥8%, is associated with increased levels and frequencies of periodontal pathogens in the subgingival biofilm of subjects with type 2 DM and ChP.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Blood Glucose/analysis , Diabetes Mellitus, Type 2/microbiology , Diabetes Mellitus, Type 2/therapy , Chronic Periodontitis/microbiology , Chronic Periodontitis/blood , Gingiva/microbiology , Colony Count, Microbial , Treatment Outcome , Statistics, Nonparametric , Biofilms , Dental Plaque/microbiology , Diabetes Mellitus, Type 2/complications , Bacterial Load , Real-Time Polymerase Chain Reaction , Gram-Negative Bacteria/isolation & purification , Hyperglycemia/prevention & control
5.
Braz. oral res. (Online) ; 31: e32, 2017. tab, graf
Article in English | LILACS | ID: biblio-839525

ABSTRACT

Abstract In recent years, different chlorhexidine formulations have been tested, including an alcohol-free alternative, but the effect of this solution on early biofilm formation is not clear. A crossover, randomized, double-blind clinical trial was conducted to evaluate the effect of two chlorhexidine solutions against supra- and subgingival biofilm formation (NCT#02656251). Thirty-five participants were randomized and asked to rinse twice daily with 15 ml of an alcohol-containing 0.12% chlorhexidine solution, an alcohol-free 0.12% chlorhexidine solution, or placebo. The study was conducted in three experimental periods of 4 days each, with a 10-day washout between the periods. All the experimental periods followed the same protocol, except that the solutions were switched. Biofilm distribution was evaluated every 24 hours by the Plaque-Free Zone Index, during 96 hours. Adverse events were self-reported and sensory evaluation was performed using a hedonic scale. Compared to the placebo, the chlorhexidine solutions resulted in a significantly higher number of surfaces free of plaque over 96 hours (p < 0.01), and were able to prevent subgingival biofilm formation (p < 0.01). The alcohol-free chlorhexidine solution was associated with a lower incidence of adverse events, compared with alcohol-containing chlorhexidine (p < 0.05); it also received better sensory evaluation and acceptance by trial participants, compared with the alcohol-containing chlorhexidine (p = 0.007), and had a similar inhibitory effect on the formation of supra- and subgingival biofilms.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Biofilms/drug effects , Chlorhexidine/chemistry , Chlorhexidine/pharmacology , Ethanol/chemistry , Ethanol/pharmacology , Mouthwashes/chemistry , Mouthwashes/pharmacology , Anti-Infective Agents, Local/chemistry , Anti-Infective Agents, Local/pharmacology , Cross-Over Studies , Dental Plaque Index , Dental Plaque/prevention & control , Double-Blind Method , Drug Combinations , Gingiva/drug effects , Gingiva/microbiology , Taste , Time Factors , Treatment Outcome
6.
J. appl. oral sci ; 24(2): 181-185, Mar.-Apr. 2016. tab, graf
Article in English | LILACS | ID: lil-779905

ABSTRACT

ABSTRACT Objective The aim of this study was to evaluate the association of Porphyromonas endodontalis, Filifactor alocis and Dialister pneumosintes with the occurrence of periodontitis. Material and Methods Thirty subjects with chronic periodontitis (ChP) and 10 with periodontal health (PH) were included in the study. Nine subgingival biofilm samples were collected as follows: i) PH group - from the mesial/buccal aspect of each tooth in two randomly chosen contralateral quadrants; ii) ChP group - from three sites in each of the following probing depth (PD) categories: shallow (≤3 mm), moderate (4-6 mm) and deep (≥7 mm). Checkerboard DNA-DNA hybridization was used to analyze the samples. Results We found the three species evaluated in a higher percentage of sites and at higher levels in the group with ChP than in the PH group (p<0.05, Mann-Whitney test). We also observed these differences when the samples from sites with PD≤4 mm or ≥5 mm of subjects with ChP were compared with those from subjects with PH (p<0.05, Mann-Whitney test). In addition, the prevalence and levels of D. pneumosintes, and especially of F. alocis were very low in healthy subjects (0.12x105 and 0.01x105, respectively). Conclusion F. alocis and D. pneumosintes might be associated with the etiology of ChP, and their role in the onset and progression of this infection should be further investigated. The role of P. endodontalis was less evident, since this species was found in relatively high levels and prevalence in the PH group.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Peptostreptococcus/pathogenicity , Porphyromonas endodontalis/pathogenicity , Veillonellaceae/pathogenicity , Chronic Periodontitis/microbiology , Peptostreptococcus/isolation & purification , Brazil , DNA, Bacterial , Colony Count, Microbial , DNA Probes , Case-Control Studies , Statistics, Nonparametric , Biofilms , Porphyromonas endodontalis/isolation & purification , Dental Plaque/microbiology , Veillonellaceae/isolation & purification , Gingiva/microbiology
7.
Braz. oral res. (Online) ; 30(1): e87, 2016. tab, graf
Article in English | LILACS | ID: biblio-952058

ABSTRACT

Abstract This study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (FISH) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. Of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. Probing depth (PD), bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected, and FISH was used to identify the numbers of eight periodontal pathogens. Friedman test was used to compare differences between follow-up examinations, followed by a multiple comparison test for a post hoc pairwise comparison. Clinically, a significantly greater number of teeth with PD = 4-5 mm were found during pregnancy than on postpartum examinations. Microbial analysis showed a statistically significant decrease in cell count over the study period for Prevotella nigrescens. P. intermedia, Campylobacter rectus, and Porphyromonas gingivalis also decrease, although not significantly, and Aggregatibacter actinomycetemcomitans increased. No significant changes were found for Fusobacterium nucleatum, Treponema denticola, or Tannerella forsythia. Our data demonstrate a change in the subgingival microbiota during pregnancy, at least for P. nigrescens.


Subject(s)
Humans , Pregnancy , Adult , Young Adult , Periodontitis/microbiology , Gestational Age , Gingiva/microbiology , Pregnancy Complications, Infectious/microbiology , Reference Values , Time Factors , Periodontium/microbiology , Periodontal Index , Longitudinal Studies , In Situ Hybridization, Fluorescence , Statistics, Nonparametric , Biofilms/growth & development , Dental Plaque/microbiology , Postpartum Period , Bacterial Load , Microbiota , Tannerella forsythia/isolation & purification , Gram-Negative Bacteria/isolation & purification
8.
Article in Spanish | LILACS | ID: lil-757877

ABSTRACT

Objetivo: Reportamos la asociación entre el polimorfismo de nucleótido simple de IL-10-592C/A (rs1800872) y la detección/abundancia relativa de los periodontopatógenos Porfiromonas gingivalis, Tenerella forsythia, Treponema denticola y Aggregatibacter actinomycetemcomitans. Además investigamos la influencia de los determinantes genéticos y microbiológicos en los niveles de expresión de IL-10 en lesiones periodontales. Metodología Fueron reclutados 117 pacientes con periodontitis crónica y 58 controles. Luego del examen clínico fueron obtenidas muestras microbiológicas y la presencia/carga bacteriana de especies de periodontopatógenos fue cuantificada por RT-PCR. El genotipo para IL-10-592C/A fue determinado mediante restriction fragment length polymorphism. Resultados La distribución alélica del SNP rs1800872 en la población investigada cumplió con el equilibrio de Hardy-Weinberg (p = 0,64). Como ya ha sido reportado, los sujetos polimórficos demostraron menor expresión de IL-10 y riesgo aumentado de sufrir periodontitis crónica. El polimorfismo IL-10-592C/A no demostró relación con la detección o carga bacteriana de ninguna de las bacterias investigadas, además los niveles de expresión de IL-10 no fueron influenciados por el perfil microbiológico, sino que se correlacionaron directamente con el genotipo para el polimorfismo IL-10-592C/A.


Objective: A study was conducted to investigate the possible influence of the single nucleotide polymorphism (SNP) IL-10-592 C/A on the occurrence and load of the periodontal pathogens: P. gingivalis, T. forsythia, T. denticolaand A. Actinomycetemcomitans; as well to investigate the influence of microbial and genetic factors on the modulation of local IL-10 mRNA levels. Methodology The study included 117 cases and 58 controls. After clinical examination microbiological samples were obtained and the detection/quantification of the target bacterial species was performed by RT-PCR. SNP rs1800872 was assayed by restriction fragment length polymorphism (RFLP). Results Allele distribution of rs1800872 was in Hardy-Weinberg equilibrium (P = 64). As previously reported, polymorphic subjects demonstrated decreased IL-10 expression and increased risk of suffering chronic periodontitis. IL-10-592C/A rs1800872 SNP was not associated with the detection or the bacterial load of the investigated pathogens. Moreover, the presence/load of bacteria at periodontal sites did not influence IL-10 expression, which was determined by the genetic background of the study subjects. IL-10-592C/A SNP was not associated with detection/bacterial load of pathogenic bacteria. IL-10 expression levels were determined by the genetic background and were independent of the bacterial microenvironment.


Subject(s)
Humans , Male , Adult , Female , Periodontal Diseases/genetics , Periodontal Diseases/microbiology , /genetics , Bacterial Load , Bacteria/isolation & purification , Case-Control Studies , DNA, Bacterial , Gingiva/microbiology , Periodontal Diseases/immunology , Host-Pathogen Interactions , /physiology , Polymorphism, Single Nucleotide , Real-Time Polymerase Chain Reaction
9.
Braz. oral res. (Online) ; 29(1): 1-7, 2015. tab, ilus
Article in English | LILACS | ID: lil-777159

ABSTRACT

The purpose of this study was to detect Candida spp. on the tongue and in the subgingival sites in healthy and type 2 diabetes (T2D) patients with chronic periodontitis (CP), and to compare the accuracy of sampling methods. This study included 131 patients divided into four groups: healthy control (group A), nondiabetics + CP (Group B), diabetics with good metabolic control + CP (group C) and diabetics with poor glycoregulation + CP (Group D). Cotton swab samples from tongue and subgingival samples were obtained from each patient with help of sterile paper points and a sterile curette. Swab cultures were made on Sabouraud dextrose agar. The number of CFUs was counted. The sampling methods for subgingival plaque were compared by Receiving Operator Curve (ROC). The presence of Candida spp. on the tongue was statistically significant among groups (group D vs. others three groups: χ2: p < 0.005 for each group). Positive findings of subgingival Candida spp. did not differ among the groups. There were no significant differences in the quantification ofCandida spp., neither on the tongue, nor in the subgingival samples. 17.2% of diabetic patients revealed the presence ofCandida spp. in the subgingival samples, with negative finding on tongue. There was a significant difference in the sampling methods for subgingival plaque (p = 0.000). Candidaspp. is more prevalent on the tongue of diabetics. The sampling of subgingival plaque by a sterile curette is more accurate than with paper points. Subgingival plaque may represent a reservoir of commensals. It is necessary to standardize the sampling of subgingival plaque.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Tongue/microbiology , Candida albicans/isolation & purification , Diabetes Mellitus, Type 2/microbiology , Chronic Periodontitis/microbiology , Gingiva/microbiology , Reference Values , Periodontium/microbiology , Colony Count, Microbial , Epidemiologic Methods , Biofilms
10.
Braz. oral res. (Online) ; 29(1): 1-8, 2015. tab, ilus
Article in English | LILACS, BNUY, BNUY-Odon | ID: lil-777184

ABSTRACT

The aim of this study was to determine the efficacy of rinses with slurries of a dentifrice containing triclosan (TCS), as compared with rinses with slurries from a control dentifrice, in controlling early subgingival biofilm formation. A double-blind, randomized and cross-over clinical trial was designed, and 26 dental students were included. In the first period, participants were randomized to rinse with a TCS slurry or a control slurry, in a 12 h interval, and to refrain from mechanical cleaning. A Plaque Free Zone Index was assessed at 24 h, 48 h, 72 h and 96 h. After a washout period of 10 days, the second experimental period was conducted, following the same protocol as the first period, except that the slurry groups were switched. Use of the TCS slurry resulted in a significantly higher percentage of plaque-free surfaces, both at 24 h and at 72 h (p < 0.01). In the of 48-72 h interval, the triclosan slurry showed a lower percentage of sites converted to a score of 2 (38.1% for the testversus 40% for the control product, p = 0.015). In conclusion, rinsing with slurries of dentifrice containing TCS retards the down growth of bacterial biofilms from the supra- to the subgingival environment.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Anti-Infective Agents, Local/therapeutic use , Biofilms/drug effects , Dental Plaque/prevention & control , Dentifrices/therapeutic use , Gingiva/microbiology , Triclosan/therapeutic use , Biofilms/growth & development , Dental Plaque Index , Double-Blind Method , Gingiva/drug effects , Periodontal Diseases/microbiology , Periodontal Diseases/prevention & control , Reproducibility of Results , Statistics, Nonparametric , Time Factors , Treatment Outcome
11.
Braz. oral res. (Online) ; 29(1): 1-6, 2015. tab
Article in English | LILACS | ID: lil-777189

ABSTRACT

This study aimed to determine the presence of Prevotella strains and genes associated with resistance to lactamics in different oral niches from patients with/without primary endodontic infections. Saliva (S) and supragingival biofilm (SB) were collected from three patient groups: Group I – no endodontic infection (n = 15); Group II – acute endodontic infection (n = 12); and Group III – chronic endodontic infection (n = 15). Root canal (RC) samples were collected from Groups II and III. The presence of P. intermedia, P nigrescens, P. tannerae and cfxA/cfxA2 gene was assessed by PCR. The cfxA/cfxA2 gene was not detected in all environments within the same patient. The cfxA/cfxA2 gene was present in 23.81% of S samples, 28.57% of SB samples, and 7.41% of RC samples. Prevotella species were detected in 53.97%, 47.62% and 34.56% of the S, SB, and RC samples, respectively. P. intermedia had a high frequency in saliva samples from Group 3. Saliva samples from Group 1 had higher detection rates of P. nigrescens than did Groups 2 and 3. Patients without endodontic disease had high frequencies of P. nigrescens in the SB samples. The presence or absence of spontaneous symptoms was not related to the detection rates for resistance genes in the RC samples. Saliva, supragingival biofilm and root canals can harbor resistant bacteria. The presence of symptomatology did not increase the presence of the cfxA/cfxA2 gene in the supragingival biofilm and inside root canals.


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Biofilms , Dental Pulp Cavity/microbiology , Gingiva/microbiology , Prevotella/isolation & purification , Saliva/microbiology , beta-Lactam Resistance/genetics , beta-Lactamases/genetics , Chi-Square Distribution , DNA, Bacterial/analysis , Polymerase Chain Reaction , Prevotella/genetics , Sequence Analysis, DNA , beta-Lactamases/analysis
12.
Braz. oral res. (Online) ; 29(1): 1-7, 2015. tab, ilus
Article in English | LILACS | ID: lil-777247

ABSTRACT

The objective of this study was to evaluate the effect of strict supragingival biofilm control on serum inflammatory markers and on periodontal clinical parameters in type 2 diabetes mellitus (T2DM) patients with chronic severe periodontitis. Twenty-four individuals with T2DM and periodontitis were randomly allocated to two treatment groups. The supragingival therapy group (ST, n = 12) received supragingival scaling, whereas the intensive therapy group (IT, n = 12) underwent supra- and subgingival scaling, as well as root planing. Patients from both groups received professional oral hygiene instructions every month. Data regarding visible plaque index (VPI), gingival bleeding index (GBI), bleeding on probing (BOP), probing pocket depth (PPD), clinical attachment level (CAL), serum levels of interleukin (IL)-6, IL-17A, IL-8, tumor necrosis factor α (TNF-α), monocyte chemoattractant protein (MCP)-1 enzyme-linked immunosorbent assay (ELISA), and glycated hemoglobin (HbA1c) levels were obtained at baseline and at 6 months post-therapy. Both therapies resulted in the improvement of almost all clinical periodontal parameters (p < 0.05). There were no differences in TNF-α, IL-8, IL-17A and HbA1c levels in either group (p >0.05), between the two periods. However, MCP-1 levels were significantly reduced in both the ST (p = 0.034) and the IT (p = 0.016) groups, whereas the serum IL-6 levels were significantly reduced only in the IT group (p = 0.001). Strict control of supragingival biofilm has a limited effect on systemic inflammatory markers, and a moderate effect on periodontal clinical parameters.


Subject(s)
Female , Humans , Male , Middle Aged , Biofilms , Chronic Periodontitis/blood , Chronic Periodontitis/therapy , Dental Scaling/methods , /blood , Gingiva/microbiology , Biomarkers/blood , /blood , Enzyme-Linked Immunosorbent Assay , Glycated Hemoglobin A/analysis , Interleukins/blood , Periodontal Index , Reference Values , Statistics, Nonparametric , Treatment Outcome , Tumor Necrosis Factor-alpha/blood
13.
J. appl. oral sci ; 22(6): 528-533, Nov-Dec/2014. tab, graf
Article in English | LILACS, BBO | ID: lil-732582

ABSTRACT

Objectivo In this study, the gingival conditions and the quantitative detection for Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia in pregnant women were determined. Material and Methods Quantitative determinations of periodontal bacteria by using a SyBr green system in women during pregnancy were performed. Women at the 2nd and 3rd trimesters of pregnancy and non-pregnant women were included in this study. A. actinomycetemcomitans was observed in high numbers in women at the 2nd and 3rd trimesters of pregnancy with a significant difference (p<0.05). F. nucleatum and P. intermedia were also observed in high levels. Results and Conclusion Our results show that pregnant women are more susceptible to gingivitis, and the presence of A. actinomycetemcomitans in subgingival biofilm might be taken into account for the treatment of periodontal disease. .


Subject(s)
Humans , Female , Pregnancy , Adolescent , Adult , Young Adult , Aggregatibacter actinomycetemcomitans/isolation & purification , Fusobacterium nucleatum/isolation & purification , Gingiva/microbiology , Periodontium/microbiology , Porphyromonas gingivalis/isolation & purification , Prevotella intermedia/isolation & purification , Bacterial Load , Biofilms/growth & development , Longitudinal Studies , Periodontal Diseases/microbiology , Periodontal Index , Polymerase Chain Reaction , Pregnancy Trimester, Second , Pregnancy Trimester, Third
14.
Braz. j. microbiol ; 45(2): 495-501, Apr.-June 2014. graf, tab
Article in English | LILACS | ID: lil-723105

ABSTRACT

P. aeruginosa and Acinetobacter spp. are important pathogens associated with late nosocomial pneumonia in hospitalized and institutionalized individuals. The oral cavity may be a major source of these respiratory pathogens, particularly in the presence of poor oral hygiene and periodontal infection. This study investigated the prevalence of P. aeruginosa and Acinetobacter spp. in subgingival biofilm and saliva of subjects with periodontal disease or health. Samples were obtained from 55 periodontally healthy (PH) and 169 chronic periodontitis (CP) patients. DNA was obtained from the samples and detection of P. aeruginosa and Acinetobacter spp. was carried out by multiplex and nested PCR. P. aeruginosa and Acinetobacter spp. were detected in 40% and 45% of all samples, respectively. No significant differences in the distribution of these microorganisms between men and women, subgingival biofilm and saliva samples, patients < 35 and > 35 years of age, and smokers and non-smokers were observed regardless periodontal status (p > 0.05). In contrast, the frequencies of P. aeruginosa and Acinetobacter spp. in saliva and biofilm samples were significantly greater in CP than PH patients (p < 0.01). Smokers presenting P. aeruginosa and high frequencies of supragingival plaque were more likely to present CP than PH. P. aeruginosa and Acinetobacter spp. are frequently detected in the oral microbiota of CP. Poor oral hygiene, smoking and the presence of P. aeruginosa are strongly associated with periodontitis.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Young Adult , Acinetobacter/isolation & purification , Biofilms/growth & development , Gingiva/microbiology , Healthy Volunteers , Periodontal Diseases/microbiology , Pseudomonas aeruginosa/isolation & purification , Saliva/microbiology , Acinetobacter/physiology , DNA, Bacterial/genetics , DNA, Bacterial/isolation & purification , Polymerase Chain Reaction , Prevalence , Pseudomonas aeruginosa/physiology
15.
Braz. oral res ; 26(5): 443-449, Sept.-Oct. 2012. ilus, tab
Article in English | LILACS | ID: lil-649363

ABSTRACT

The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.


Subject(s)
Adult , Female , Humans , Pregnancy , Young Adult , Bacteria/growth & development , Bacterial Load/methods , Biofilms/growth & development , Gingiva/microbiology , Cross-Sectional Studies , Dental Health Surveys , In Situ Hybridization, Fluorescence/methods , Periodontal Diseases/microbiology , Pregnancy Complications, Infectious/microbiology , Statistics, Nonparametric
16.
Braz. oral res ; 26(4): 366-372, July-Aug. 2012. graf, tab
Article in English | LILACS | ID: lil-640713

ABSTRACT

This study investigated the effect of non-surgical periodontal therapy on the composition of subgingival microbiota of patients with chronic kidney disease (CKD). Sixteen CKD pre-dialysis individuals (CKD) and 14 individuals without clinical evidence of kidney disease (C) presenting chronic periodontitis were treated by scaling and root planing. Subgingival samples were collected from each patient and analyzed for their composition by checkerboard at baseline and 3 months post-therapy. Significant differences between groups at baseline were sought by the Mann-Whitney and χ² tests. Changes over time were examined by the Wilcoxon test. At baseline, the CKD group had significantly lower counts of E. faecalis compared to the C group (p < 0.05). After treatment, the levels of a greater number of species were reduced in the C group. Higher levels of A. israelii, C. rectus, F. periodonticum, P. micra, P. nigrescens, T. forsythia, N. mucosa, and S. anginosus (p < 0.05) were found in the CKD group compared to the C group. Also, non-responsive sites in CKD individuals harbored significantly higher levels of pathogenic species (T. forsythia, P. gingivalis, T. denticola, Fusobacterium spp., D. pneumosintes, E. faecalis and S. aureus; p < 0.05) than sites that responded to therapy, as well as non-responsive sites in the C group. The periodontitis-associated subgingival microbiota of CKD and systemically healthy individuals was similar in composition. However, high levels of pathogenic species persisted in the subgingival microbiota of patients with CKD after treatment.


Subject(s)
Aged , Female , Humans , Middle Aged , Gingiva/microbiology , Periodontitis/therapy , Renal Insufficiency, Chronic/microbiology , Bacterial Load , Chronic Disease , Dental Scaling , DNA Probes , Metagenome , Periodontitis/immunology , Renal Insufficiency, Chronic/immunology , Statistics, Nonparametric , Time Factors , Treatment Outcome
17.
J. appl. oral sci ; 19(1): 68-73, Jan.-Feb. 2011. graf, tab
Article in English | LILACS | ID: lil-578751

ABSTRACT

OBJECTIVES: This study evaluated the effects of coronally positioned flap (CPF) on the subgingival biofilm composition. MATERIAL AND METHODS: Twenty-two subjects with gingival recessions were treated with CPF. Clinical parameters were assessed before and at 6 months after surgery. Subgingival biofilms were analyzed by checkerboard DNA-DNA hybridization technique for 40 bacterial species. RESULTS: Recession height, clinical attachment level and bleeding on probing improved significantly (p<0.05) at 6 months post-CPF. The proportions of 10 periodontal pathogens and the proportions of red and orange complexes decreased at 6 months. CONCLUSION: In conclusion, CPF can induce beneficial effects on the composition of the subgingival microbiota after 6 months.


Subject(s)
Adult , Female , Humans , Male , Middle Aged , Biofilms/growth & development , Gingiva/microbiology , Gingival Recession/microbiology , Surgical Flaps/microbiology , Bacterial Load , DNA Probes , DNA, Bacterial/genetics , Gingiva/surgery , Gingival Recession/surgery , Metagenome , Statistics, Nonparametric , Time Factors
18.
J. appl. oral sci ; 18(4): 426-431, July-Aug. 2010. graf, tab
Article in English | LILACS | ID: lil-557116

ABSTRACT

OBJECTIVE: The aim of this study was to detect the prevalence of selected bacterial species in intraoral sites of patients with chronic periodontitis (CP) using multiplex polymerase chain reaction (PCR). METHODOLOGY: Samples were collected from the tongue dorsum, buccal mucosa, supragingival and subgingival plaque and saliva of 30 patients with untreated CP. Multiplex PCR was used to determine prevalence rates, which were then compared using a chi-square test. Significance level was set at p<0.05. Mean and standard deviation values were used to evaluate variations in prevalence according to site. RESULTS: The prevalence of S. mutans was 70 percent in saliva; 60 percent in samples collected from the tongue dorsum; 50 percent in samples collected from the buccal mucosa; 56.5 percent in the supragingival plaque; and 53.5 percent in the subgingival plaque. The prevalence of E. faecalis ranged from 3.5 percent to 13.5 percent in all intraoral microenvironment. The highest prevalence of P. gingivalis was found in subgingival plaque (53.5 percent), and of P. intermedia in supragingival plaque (33.5 percent), subgingival plaque (30 percent) and tongue dorsum (33.5 percent). The prevalence of bacteria did not vary significantly among the intraoral sites. CONCLUSIONS: All studied bacteria were identified in intraoral sites. S. mutans, P. gingivalis and P. intermedia had high prevalence rates, but the prevalence of E. faecalis was low. Multiplex PCR proved to be an adequate method for epidemiological studies.


Subject(s)
Adult , Female , Humans , Male , Bacteroidaceae/classification , Chronic Periodontitis/microbiology , Lactobacillales/classification , Polymerase Chain Reaction/methods , Cheek/microbiology , Chronic Periodontitis/classification , Dental Plaque/microbiology , Enterococcus faecalis/isolation & purification , Gingiva/microbiology , Gingival Hemorrhage/classification , Mouth Mucosa/microbiology , Periodontal Pocket/classification , Porphyromonas gingivalis/isolation & purification , Prevotella intermedia/isolation & purification , Saliva/microbiology , Streptococcus mutans/isolation & purification , Tongue/microbiology
19.
Article in Spanish | LILACS | ID: lil-627526

ABSTRACT

Uno de los campos de interés en el estudio de la microbiología periodontal para muchos investigadores ha sido identificar si es posible que los microorganismos responsables del origen y progresión de la enfermedad periodontal que habitan sobre el margen gingival (supragingival) y bajo este (subgingival) tengan una relación directa que permita mantener interacciones influyentes en el crecimiento y desarrollo de las diferentes especies bacterianas que habitan en los tejidos periodontales. Por lo tanto, al remover los microorganismos que se ubican supragingivalmente sería posible encontrar cambios en el medio subgingival al no existir un intercambio entre los ambientes aerobios (supragingival) y anaerobios (subgingival) una vez desorganizada la placa bacteriana supragingival. Para demostrar esta relación se seleccionaron 7 individuos con diagnóstico de periodontitis crónica moderada y severa a los cuales se les realizó un destartraje supragingival de boca completa para lograr desorganizar la placa bacteriana supragingival. A su vez se tomaron muestras microbiológicas de los sacos periodontales más profundos de cada cuadrante de estos individuos, siendo la primera muestra tomada previo al destartraje supragingival considerada como muestra basal (día 0), luego se tomaron a las 24 horas, a los 7 y 21 días de removida la placa bacteriana supragingival. De los resultados del presente estudio pudimos concluir que al desorganizar el biofilm supragingival se observa una disminución en la cantidad total de microorganismos subgingivales, así como también disminuye de manera considerable la proporción de Porphyoromona gingivalis presente en el medio subgingival. Lo cual permitiría establecer la existencia de una relación directa y dependiente entre los microorganismos que habitan el medio supragingival y subgingival.


One of the fields of interest in the study of the microbiology periodontal for many investigators has been to identify if it is possible that the microorganisms responsible for the origin and progression of the disease periodontal that live on the margin gingival (supragingival) and under this (subgingival) they have a direct relation that allows to support influential interactions in the growth and development of the different bacterial species that they live in the tissue periodontal.Therefore having removed the microorganisms that are located supragingivalmente would be possible to find changes in the way subgingival when an exchange not to exist between the aerobic environments (supragingival) and anaerobic (subgingival) once disorganized the bacterial plate supragingival. To demonstrate this relation 7 individuals selected with diagnosis of periodontitis chronicle moderate and severe to which they there was realized a destartraje supragingival of complete mouth to achieve supragingival to disorganize the bacterial plate. In turn microbiological samples of the sacks took periodontales deeper of every quadrant of these individuals, being the first taken sample before the destartraje supragingival considered as sample basal (the 0th), then they took at to 1, 7 and 21 days of removed the bacterial plate supragingival anaerobios (subgingival) once disorganized the bacterial plate supragingival Of the results of the present study we could conclude that on having disorganized the biofilm supragingival a decrease is observed in the total quantity of microorganisms subgingivales, as well as also it diminishes in a considerable way the proportion of present Porphyoromona gingivalis in the way subgingival. Which would lead to thinking that there exists a direct and dependent relation between the microorganisms that live the way supragingival and subgingival.


Subject(s)
Aged , Dental Scaling , Chronic Periodontitis/microbiology , Chronic Periodontitis/therapy , Dental Plaque/microbiology , Porphyromonas gingivalis/isolation & purification , Colony Count, Microbial , Gingiva/microbiology , Dental Plaque/therapy , Time Factors
20.
Egyptian Journal of Medical Laboratory Sciences. 2010; 19 (1): 47-54
in English | IMEMR | ID: emr-126617

ABSTRACT

The mouth is the portal of entry of food this provides access to a wide array of microbes, the majority of which become part of the transient oral flora. Microbiological studies on samples taken from gingival pockets surrounds overdenture abutment teeth revealed different types of bacteria. The design of the overdenture prosthesis and supporting abutments affect the nature and quantity of bacterial found and that will be revealed in this study. This study was conducted to compare the microbiological changes occurring in gingival and periodontal tissue and gingival crevicular fluid as indicators of the condition of periodontium around abutments with different designs of abutment teeth of tooth supported mandibular overdenture. Subgingival bacteriological samples were taken from abutment's gingival crevice at the time of denture insertion and after three months to evaluate the effect of abutments design on the bacterial types as well as counts. The collected samples were cultured, identified and counted. A total of 20 male patients were divided into two equal groups according to abutment's length [Group I and II]. The patients' abutments were subdivided into two equal groups according to abutment's covering [A and B]. This study revealed an increase in anaerobic bacteria in both groups, with significant increase in Group I-A [patients rehabilitated with long abutments with copings] while all other groups have shown to significant difference before and after denture insertion. Insertion of overdenture accompanied with increase of anaerobic bacteria


Subject(s)
Humans , Male , Dental Abutments/microbiology , Gingiva/microbiology , Bacteria, Anaerobic
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