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1.
Rev. Assoc. Med. Bras. (1992) ; 66(11): 1560-1565, Nov. 2020. tab, graf
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1143635

ABSTRACT

SUMMARY BACKGROUND: This study aimed to investigate the deletion polymorphisms of the genes of the glutathione S-transferase family GSTT1 and GSTM1 in patients with Polycystic Ovarian Syndrome (PCOS), comparing them with a control population. METHODS: Blood was collected from 219 women (110 with PCOS and 109 controls) and genomic DNA was extracted. For the analysis of polymorphisms, the technique used was multiplex PCR. In the statistical analysis, the chi-square test and multiple logistic regression were used. RESULTS: There is no association between the GSTM1 null and GSTT1 null genotypes with PCOS when analyzed separately (P = 0.616 and P = 0.188). The analysis of the combined genotypes showed differences between the groups (P < 0.05), evidencing that the genotypic combination GSTT1 positive and GSTM1 negative is more frequent among patients. In the multivariate analysis, smoking was more frequent in the control group (OR = 0.22; 95% CI - 0.87-0.57; P = 0.002) while the presence of a family history of PCOS (OR = 2, 96; 95% CI - 1.54-5.68; P = 0.001) was more frequent in women with PCOS. CONCLUSIONS: In the studied sample, the deletion polymorphisms of the GSTT1 and GSTM1 genes isolated are not associated with PCOS, but in combination, they may be implicated in the etiology of the condition.


RESUMO OBJETIVO: Este estudo teve como objetivo investigar os polimorfismos de deleção dos genes da família glutationa S-transferase GSTT1 e GSTM1 em pacientes com síndrome dos ovários policísticos (SOP), comparando-as com uma população controle. MÉTODOS: Foi colhido sangue de 219 mulheres (110 com SOP e 109 controles) e extraído o DNA genômico. Para análise dos polimorfismos, a técnica empregada foi PCR multiplex. Na análise estatística foi utilizado o teste do qui-quadrado e regressão logística múltipla. RESULTADOS: Não há associação dos genótipos GSTM1 nulo e GSTT1 nulo com SOP quando analisados isoladamente (p=0,616 e p=0,188). A análise dos genótipos combinados mostrou diferenças entre os grupos (p<0,05), evidenciando que a combinação genotípica GSTT1 positivo e GSTM1 negativo é mais frequente entre as pacientes. Na análise multivariada, o hábito tabagista foi mais frequente no grupo controle (OR=0,22; IC 95% - 0,87-0,57; p=0,002), enquanto que a presença do histórico de SOP familiar (OR=2,96; IC 95% - 1,54-5,68; p=0,001) foi mais frequente nas mulheres com SOP. CONCLUSÕES: Na casuística estudada, os polimorfismos de deleção dos genes GSTT1 e GSTM1 isolados não estão associados a SOP, mas em combinação podem estar implicados na etiologia da condição.


Subject(s)
Humans , Female , Polycystic Ovary Syndrome/genetics , Glutathione Transferase/genetics , Case-Control Studies , Risk Factors , Genetic Predisposition to Disease , Genotype
2.
Arq. bras. med. vet. zootec. (Online) ; 72(4): 1413-1423, July-Aug. 2020. tab, graf, ilus, mapas
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1131511

ABSTRACT

Objetivou-se neste estudo analisar biomarcadores histológicos e bioquímicos em brânquias de U. cordatus indicativos de impactos na Baía de São Marcos. Caranguejos foram coletados em quatro áreas na Baía de São Marcos: A1= Ilha dos Caranguejos (com baixo impacto); A2= Coqueiro, A3= Porto Grande, A4= Cajueiro (áreas potencialmente impactadas). Mediram-se os dados biométricos de cada exemplar de caranguejo. Amostras de brânquias foram submetidas à técnica histológica padrão e homogeneizadas em tampão fosfato, e o sobrenadante foi utilizado para análise das enzimas glutationa-S-transferase (GST) e catalase (CAT). A biometria indicou que os caranguejos de A1 são significativamente (P<0,05) maiores e mais pesados do que os caranguejos das áreas A2, A3 e A4. As alterações branquiais (rompimento das células pilastras, deformação do canal marginal, deslocamento da cutícula e necrose) foram significativamente (P˂0,05) mais frequentes em caranguejos de A2, A3 e A4 do que nos caranguejos de A1. As atividades enzimáticas da GST e CAT nos caranguejos apresentaram diferença significativa (P<0,05) entre as áreas de coletas, com padrão similar ao observado para as alterações branquiais. Os biomarcadores analisados mostraram que os caranguejos estão sob diferentes níveis de impactos (A4>A3>A2>A1) ao longo da Baía de São Marcos.(AU)


The objective of this study was to analyze histological and biochemical biomarkers in U. cordatus gills indicative of impacts in São Marcos Bay. Crabs were collected from four areas in São Marcos Bay: A1=Ilha dos Caranguejos (with low impact); A2=Coqueiro, A3=Porto Grande, A4=Cajueiro (potentially impacted areas). The biometric data of each specimen was measured. Gill samples were submitted to standard histological technique and homogenized in phosphate buffer, and the supernatant was used for analysis of glutathione S-transferase (GST) and catalase (CAT) enzyme activity. Biometric data indicated that crabs found in A1 are significantly (P<0.05) larger and heavier than crabs found in A2, A3 and A4 areas. Gill alterations (rupture of pilaster cells, Dilation of the marginal channel, Cuticle Rupture and necrosis) were significantly (P˂0.05) more frequent in the crabs in A2, A3 and A4 than crabs in A1. The enzymatic activities of GST and CAT showed significant difference (P<0.05) between the sampling areas, similar to that observed for gill alterations. The biomarkers analyzed showed that the crabs are under different impact levels (A4> A3> A2> A1) along the São Marcos Bay.(AU)


Subject(s)
Animals , Biomarkers/analysis , Xenobiotics , Catalase , Brachyura/anatomy & histology , Gills/anatomy & histology , Glutathione Transferase , Brazil , Environment
3.
Braz. arch. biol. technol ; 63: e20180571, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132192

ABSTRACT

Abstract Rice (Oryza sativa L.) is one of the most important crops in the world, and it is considered the primary source of nutritional layout in developing countries in Asia. The glutathione S-transferases (GSTs) superfamily confers to rice protection against biotic and abiotic stress, and herbicide resistance. However, the three-dimensional structure of a GST Tau class, is unsolved. The objectives of this work were to develop a reliable comparative model for the s-transferase glutathione class Tau 4 from rice, and simulate docking interactions, against herbicides bentazon and metsulfuron. Results showed that the predicted model is reliable and has structural quality. Ramachandran plot set 91.9% of the residues in the most favored regions. All complexes showed negative binding energies values; and metsulfuron docked to the glutathione tripeptide, and it represents a possible insilico evidence of glutathione conjugation with this herbicide.


Subject(s)
Oryza/enzymology , Stress, Physiological , Glutathione Transferase/metabolism , Herbicides/metabolism , Oryza/drug effects , Inactivation, Metabolic
4.
Revista Digital de Postgrado ; 8(2): e161, ago. 2019. tab
Article in Spanish | LILACS, LIVECS | ID: biblio-1008415

ABSTRACT

En vista de la alta prevalencia del cáncer de próstata en la población venezolana y la ausencia de un patrón genético conocido en relación a la expresión de las enzimas Glutatión S-transferasas, se estudió la relación entre la expresión de un polimorfismo nulo de estas enzimas y la presencia de cáncer de adenocarcinoma prostática Métodos: Se incluyen 100 individuos para el muestreo no probabilístico, 50 pacientes con diagnóstico de adenocarcinoma de próstata comprobado mediante biopsia y 50 controles con hiperplasia prostática benigna demostrada mediante tacto y corroborada por ultrasonido transrectal, provenientes de los principales hospitales del país, se procedió a tomar muestra de sangre y mediante reacción de cadena de polimerasa, se determinó la presencia o ausencia de los genes para las enzimas Glutatión S-transferasa Mu 1 (GST M) y glutatión S-transferasa theta 1 (GST T1). Resultados: se logró evidenciar que el genotipo nulo se encontró en 40 y 24% de los pacientes mientras que para los controles fue de 38% y 22% respectivamente, demostrando que en la población venezolana estudiada no existen diferencias significativas entre casos y controles. Conclusiones: No se pudo demostrar una diferencia significativa entre los dos grupos estudiados. Recomendaciones: A pesar de nuestros hallazgos, se necesitan estudios futuros con muestras de mayor tamaño para dilucidar la posible asociación entre este patrón enzimático con el riesgo de presentar cáncer de próstata(AU)


Prostate cancer presents with a high incidence in the Venezuelan population. there is no known genetic pattern related to the expression of drug metabolizing enzymes Glutathione S-transferases. Methods: We proceeded to study the possible correlation between null polymorphism for these enzymes and prostate adenocarcinoma. the sample included 100 patients recruited from the Urology Department of three University Hospitals in Caracas, Venezuela, 50 cancer patients and 50 cancer free controls. Blood samples were drawn from each patient and polymorphisms for Glutathione S-transferase Mu 1 (GST M1) and Glutathione-sS-transferase theta 1(GST T1) were determined by polymerase chain reaction from lymphocytes. Results: Null genotype was found in 40% and 24% of cancer patients whereas the percentage in controls was 38 and 22% respectively, showing no statistically significant differences between the two groups. Conclusions: It was not possible to show a significant difference between the two groups. Recommendations: Due to the small size of the sample, it would be necessary to explore further in a larger population sample to determine whether there is an association between the expression of these enzymes and prostate cancer(AU)


Subject(s)
Humans , Male , Prostatic Hyperplasia/diagnosis , Prostatic Neoplasms/enzymology , Prostatic Neoplasms/pathology , Prostate-Specific Antigen , Ultrasound, High-Intensity Focused, Transrectal/methods , Glutathione Transferase
5.
Article in English | WPRIM | ID: wpr-760609

ABSTRACT

BACKGROUND/OBJECTIVES: Myocardial infarction (MI) is caused by extensive myocardial damage attributed to the occlusion of coronary arteries. Our previous study in a rat model of ischemia/reperfusion (I/R) demonstrated that administration of arabinoxylan (AX), comprising arabinose and xylose, protects against myocardial injury. In this study, we undertook to investigate whether psyllium seed husk (PSH), a safe dietary fiber containing a high level of AX (> 50%), also imparts protection against myocardial injury in the same rat model. MATERIALS/METHODS: Rats were fed diets supplemented with PSH (1, 10, or 100 mg/kg/d) for 3 d. The rats were then subjected to 30 min ischemia through ligation of the left anterior descending coronary artery, followed by 3 h reperfusion through release of the ligation. The hearts were harvested and cut into four slices. To assess infarct size (IS), an index representing heart damage, the slices were stained with 2,3,5-triphenyltetrazolium chloride (TTC). To elucidate underlying mechanisms, Western blotting was performed for the slices. RESULTS: Supplementation with 10 or 100 mg/kg/d of PSH significantly reduces the IS. PSH supplementation (100 mg/kg/d) tends to reduce caspase-3 generation and increase BCL-2/BAX ratio. PSH supplementation also upregulates the expression of nuclear factor erythroid 2-related factor 2 (NRF2), and its target genes including antioxidant enzymes such as glutathione S-transferase mu 2 (GSTM2) and superoxide dismutase 2 (SOD2). PSH supplementation upregulates some sirtuins (NAD+-dependent deacetylases) including SIRT5 (a mitochondrial sirtuin) and SIRT6 and SIRT7 (nuclear sirtuins). Finally, PSH supplementation upregulates the expression of protein kinase A (PKA), and increases phosphorylated cAMP response element-binding protein (CREB) (pCREB), a target protein of PKA. CONCLUSIONS: The results from this study indicate that PSH consumption reduces myocardial I/R injury in rats by inhibiting the apoptotic cascades through modulation of gene expression of several genes located upstream of apoptosis. Therefore, we believe that PSH can be developed as a functional food that would be beneficial in the prevention of MI.


Subject(s)
Animals , Apoptosis , Arabinose , Blotting, Western , Caspase 3 , Coronary Vessels , Cyclic AMP Response Element-Binding Protein , Cyclic AMP-Dependent Protein Kinases , Diet , Dietary Fiber , Functional Food , Gene Expression , Glutathione Transferase , Heart , Infarction , Ischemia , Ligation , Models, Animal , Myocardial Infarction , Psyllium , Rats , Reperfusion , Sirtuins , Superoxide Dismutase , Xylose
6.
Article in English | WPRIM | ID: wpr-786128

ABSTRACT

BACKGROUND: The worldwide incidence of squamous cell carcinoma of the tongue (SCCOT) in young patients has been increasing. We investigated clinicopathologic features of this unique population and compared them with those of SCCOT in the elderly to delineate its pathogenesis.METHODS: We compared clinicopathological parameters between patients under and over 45 years old. Immunohistochemical assays of estrogen receptor, progesterone receptor, androgen receptor, p53, p16, mdm2, cyclin D1, and glutathione S-transferase P1 were also compared between them.RESULTS: Among 189 cases, 51 patients (27.0%) were under 45 years of age. A higher proportion of women was seen in the young group, but was not statistically significant. Smoking and drinking behaviors between age groups were similar. Histopathological and immunohistochemical analysis showed no significant difference by age and sex other than higher histologic grades observed in young patients.CONCLUSIONS: SCCOT in young adults has similar clinicopathological features to that in the elderly, suggesting that both progress via similar pathogenetic pathways.


Subject(s)
Aged , Carcinoma, Squamous Cell , Cyclin D1 , Drinking , Drinking Behavior , Epithelial Cells , Estrogens , Female , Glutathione Transferase , Humans , Immunohistochemistry , Incidence , Mouth Neoplasms , Receptors, Androgen , Receptors, Progesterone , Smoke , Smoking , Tongue , Young Adult
7.
Rev. Col. Bras. Cir ; 46(1): e2068, 2019. tab, graf
Article in Portuguese | LILACS | ID: biblio-990362

ABSTRACT

RESUMO Objetivo: comparar o polimorfismo dos genes Glutationa S-transferase teta 1 (GSTT1) e Glutationa S-transferase mu 1 (GSTM1) da área do tumor com as margens proximal e distal de espécimes de estômago ressecados de pacientes com câncer gástrico, e investigar a presença do DNA do vírus Epstein-Barr (EBV) e Helicobacter pylori. Métodos: coletamos prospectivamente amostras teciduais da área do tumor e das margens de ressecção proximal e distal dos estômagos de dez pacientes com adenocarcinoma gástrico submetidos à gastrectomia com linfadenectomia D2 e submetemos esses espécimes à extração de DNA. Comparamos a área do tumor com as margens proximal e distal dos estômagos ressecados para o polimorfismo dos genes GSTT1 e GSTM1 e investigamos a presença de DNA do EBV e H. pylori. Utilizamos o exon 5 do gene p53 como controle interno da reação de PCR multiplex. Resultados: em um paciente, detectamos genótipos GSTT1 e GSTM1 nulos na área do tumor, em contraste com a presença de ambos os genes nas margens proximal e distal. Encontramos DNA do EBV e H. pylori na área do tumor e também nas margens proximal e distal. Em outro paciente, a margem proximal foi negativa para GSTT1 e o DNA do EBV foi negativo na margem distal. Em três pacientes, o EBV-DNA foi negativo apenas na margem distal. Conclusão: este é o primeiro relato em que diferentes genótipos, infecção por EBV-DNA e H. pylori foram observados no mesmo paciente, indicando provável deleção desses genes em resposta à progressão tumoral e heterogeneidade intratumoral.


ABSTRACT Objective: to compare the polymorphism of the Glutathione S-transferase theta 1 (GSTT1) and Glutathione S-transferase mu 1 (GSTM1) genes from the tumor area with the proximal and distal margins of stomach specimens resected from patients with gastric cancer, and to investigate the presence of Epstein-Barr virus (EBV) DNA and Helicobacter pylori. Methods: we prospectively collected tissue specimens from the tumor area and from the proximal and distal resection margins of the stomachs of ten patients with gastric adenocarcinoma who underwent gastrectomy with D2 lymphadenectomy, and submitted these specimens to DNA extraction. We compared the tumor area with the proximal and distal margins of the resected stomachs for polymorphism of GSTT1 and GSTM1 genes and investigated the presence of EBV-DNA and H. pylori. We used the p53 exon 5 gene as an internal control of the multiplex PCR reaction. Results: in one patient, we detected null GSTT1 and GSTM1 genotypes in the tumor area, in contrast to the presence of both genes in the proximal and distal margins. We found EBV-DNA and H. pylori in the tumor area and also in the proximal and distal margins. In another patient, the proximal margin was negative for GSTT1, and EBV-DNA was negative in the distal margin. In three patients, EBV-DNA was negative only in the distal margin. Conclusion: this is the first report where different genotypes, EBV-DNA and H. pylori infection were observed in the same patient, indicating a probable deletion of these genes in response to tumor progression and intratumoral heterogeneity.


Subject(s)
Humans , Male , Female , Aged , Polymorphism, Genetic/genetics , Stomach Neoplasms/surgery , Adenocarcinoma/surgery , Helicobacter pylori/genetics , Herpesvirus 4, Human/genetics , Stomach Neoplasms/enzymology , Stomach Neoplasms/microbiology , Stomach Neoplasms/virology , Adenocarcinoma/enzymology , Adenocarcinoma/microbiology , Adenocarcinoma/virology , Polymerase Chain Reaction , Prospective Studies , Risk Factors , Helicobacter pylori/isolation & purification , Herpesvirus 4, Human/isolation & purification , Genotype , Glutathione Transferase/genetics , Middle Aged
8.
J. bras. pneumol ; 45(3): e20170164, 2019. tab, graf
Article in English | LILACS | ID: biblio-1012550

ABSTRACT

ABSTRACT Objective: To evaluate the pulmonary alterations of animals with Hepatopulmonary Syndrome (HPS) submitted to Biliary Duct Ligature (BDL), as well as the antioxidant effect of Melatonin (MEL). Methods: Sixteen male Wistar rats, divided into four Sham groups: BDL group, Sham + MEL group and BDL + MEL. The pulmonary and hepatic histology, lipoperoxidation and antioxidant activity of lung tissue, alveolar-arterial O2 difference and lung / body weight ratio (%) were evaluated. Results: When comparing the groups, could be observed an increase of vasodilation and pulmonary fibrosis in the BDL group and the reduction of this in relation to the BDL + MEL group. It was also observed significant changes in the activity of catalase, ApCO2, ApO2 in the LBD group when compared to the other groups. Conclusion: The use of MEL has been shown to be effective in reducing vasodilation, fibrosis levels and oxidative stress as well as gas exchange in an experimental HPS model.


RESUMO Objetivo: Avaliar as alterações pulmonares de animais com Síndrome Hepatopulmonar (SHP), submetidos à ligadura de ducto biliar (LDB), bem como o efeito antioxidante da Melatonina (MEL). Métodos: Dezesseis ratos machos da espécie Wistar, divididos em quatro grupos: Sham, Grupo LDB, Grupo Sham + MEL e LDB + MEL. Foram avaliadas a histologia pulmonar e hepática, a lipoperoxidação e atividade antioxidante do tecido pulmonar, diferença álveolo-arterial de O2 e relação peso pulmonar/peso corporal (%). Resultados: Quando comparados os grupos, observamos um aumento da vasodilatação e fibrose pulmonar no grupo LDB e a redução deste em relação ao grupo LDB+MEL. Observamos ainda alterações significativas na atividade da catalase, PaCO2, PaO2 no grupo LBD quando comparado aos demais grupos. Conclusões: A utilização da MEL demonstrou-se eficaz na redução da vasodilatação, níveis de fibrose e estresse oxidativo assim como na troca gasosa em modelo experimental de SHP.


Subject(s)
Animals , Male , Hepatopulmonary Syndrome/drug therapy , Lung/drug effects , Melatonin/pharmacology , Antioxidants/pharmacology , Bile Ducts/surgery , Blood Gas Analysis , Lipid Peroxidation/drug effects , Catalase/analysis , Hepatopulmonary Syndrome/physiopathology , Hepatopulmonary Syndrome/pathology , Disease Models, Animal , Arterial Pressure/drug effects , Glutathione Transferase/analysis , Ligation , Liver/drug effects , Liver/pathology
10.
An. bras. dermatol ; 93(1): 54-58, Jan.-Feb. 2018. tab, graf
Article in English | LILACS | ID: biblio-887145

ABSTRACT

Abstract: Background: The association of glutathione S-transferases M1/T1 (GSTM1/T1) null polymorphisms with vitiligo was proposed in several studies including two Egyptian studies with contradictory results. Objective: The aim here was to assess the association between GSTM1/T1 null polymorphisms and the susceptibility to vitiligo in a larger sample of Egyptian patients with generalized vitiligo. Methods: This study included 122 vitiligo patients and 200 healthy controls that were age, and gender matched. Assessment of GSTM1/T1 gene polymorphisms was done using a multiplex polymerase chain reaction (PCR). Results: Increased odds of generalized vitiligo was observed with the null genotypes of GSTM1 and GSTT1 polymorphisms (P<0.05). Controls with GSTM1 null/GSTT1+ heterozygosis presented with a 2.97 odds protection from having generalized vitiligo (OR=2.97, 95%CI=1.1-7.7) (P=0.02) compared with patients. Study Limitations: Small sample size of patients. Conclusions: This study showed a significant trend towards an association with the combination of the GSTM1/GSTT1 double null polymorphism and generalized vitiligo. Individuals with GSTM1 null/GSTT1+ heterozygosis have a 2.97 odds protection from having generalized vitiligo compared with patients. It was is the first time, to our knowledge, that such an association has been reported.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Young Adult , Polymorphism, Genetic/genetics , Vitiligo/genetics , Genetic Predisposition to Disease/genetics , Glutathione Transferase/genetics , Case-Control Studies , Egypt , Gene Frequency , Genotype
11.
Article in English | WPRIM | ID: wpr-742239

ABSTRACT

Due to the critical location and physiological activities of the retinal pigment epithelial (RPE) cell, it is constantly subjected to contact with various infectious agents and inflammatory mediators. However, little is known about the signaling events in RPE involved in Toxoplasma gondii infection and development. The aim of the study is to screen the host mRNA transcriptional change of 3 inflammation-related gene categories, PI3K/Akt pathway regulatory components, blood vessel development factors and ROS regulators, to prove that PI3K/Akt or mTOR signaling pathway play an essential role in regulating the selected inflammation-related genes. The selected genes include PH domain and leucine- rich-repeat protein phosphatases (PHLPP), casein kinase2 (CK2), vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), glutamate-cysteine ligase (GCL), glutathione S-transferase (GST), and NAD(P)H: quinone oxidoreductase (NQO1). Using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), we found that T. gondii up-regulates PHLPP2, CK2β, VEGF, GCL, GST, and NQO1 gene expression levels, but down-regulates PHLPP1 and PEDF mRNA transcription levels. PI3K inhibition and mTOR inhibition by specific inhibitors showed that most of these host gene expression patterns were due to activation of PI3K/Akt or mTOR pathways with some exceptional cases. Taken together, our results reveal a new molecular mechanism of these gene expression change dependent on PI3K/Akt or mTOR pathways and highlight more systematical insight of how an intracellular T. gondii can manipulate host genes to avoid host defense.


Subject(s)
Blood Vessels , Caseins , Epithelial Cells , Gene Expression , Glutamate-Cysteine Ligase , Glutathione Transferase , Hydrogen-Ion Concentration , Phosphoprotein Phosphatases , Polymerase Chain Reaction , Reactive Oxygen Species , Retinaldehyde , Reverse Transcription , RNA, Messenger , Signal Transduction , Toxoplasma , Toxoplasmosis , Vascular Endothelial Growth Factor A
12.
Article in English | WPRIM | ID: wpr-713829

ABSTRACT

BACKGROUND/OBJECTIVES: Glutathione s-transferase (GST) is involved in the formation of a multigene family comprising phase II detoxification enzymes, involved in the detoxification of reactive oxygen species. This study evaluated whether daily supplementation with kale juice could modulate levels of plasma antioxidant vitamins and oxidative stress-related parameters. We further examined whether this modulation was affected by combined GSTM1 and T1 polymorphisms. SUBJECTS/METHODS: Totally, 84 subclinical hypertensive patients having systolic blood pressure (BP) over 130 mmHg or diastolic BP over 85 mmHg, received 300 mL of kale juice daily for 6 weeks. Blood samples were drawn before start of study and after completion of 6 weeks. RESULTS: After supplementation, we observed significant decrease in DNA damage and increase in erythrocyte catalase activity in all genotypes. Plasma level of vitamin C was significantly increased in the wild/null and double null genotypes. The plasma levels of β-carotene, erythrocyte glutathione peroxidase activity, and nitric oxide were increased only in the wild/null genotype after kale juice supplementation. CONCLUSIONS: The effect of kale juice was significantly greater in the GSTM1 null genotype and wild/null genotype groups, suggesting possibility of personalized nutritional prescriptions based on personal genetics.


Subject(s)
Ascorbic Acid , Blood Pressure , Brassica , Catalase , DNA Damage , Erythrocytes , Genetics , Genotype , Glutathione Peroxidase , Glutathione Transferase , Glutathione , Humans , Hypertension , Metabolic Detoxication, Phase II , Multigene Family , Nitric Oxide , Oxidative Stress , Plasma , Prescriptions , Reactive Oxygen Species , Vitamins
13.
Article in English | WPRIM | ID: wpr-715045

ABSTRACT

The purpose of the study was to investigate the effects of lipid-coated ZnO (LCZ) and the level of LCZ compared with ordinary zinc oxide (ZnO) on antioxidant defense system in the intestine and liver of piglets. A total of forty piglets (n=8) were fed a diet supplemented with 100 ppm Zn with ZnO (ZnO-1), 2,500 ppm Zn with ZnO (ZnO-2), 100 ppm Zn as LCZ (LCZ-1), 200 ppm Zn as LCZ (LCZ-2), or 400 ppm Zn as LCZ (LCZ-3) for 14-d, respectively. The LCZ-3 group resulted in higher (P < 0.05) mRNA expressions and activities of CuZn-superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), and glutathione S-transferase (GST) in jejunal mucosa compared with the ZnO-1 and LCZ-1 groups, while no difference was observed in the mRNA level of antioxidant genes between the ZnO-1 and ZnO-2 groups. Within the LCZ groups, the LCZ level linearly and quadratically (P < 0.01) increased antioxidant enzymes in the jejunum. The maximum response of jejunal antioxidant enzymes to Zn supplementation was achieved by 400 ppm of LCZ. Hepatic mRNA expression of antioxidant enzymes was unaffected by Zn source and level, while hepatic SOD and GST activities were greater (P < 0.05) in the LCZ-3 group than in the ZnO-1 group. No difference was observed in lipid peroxidation of the jejunum and liver and the total antioxidant power of plasma among groups. In conclusion, a supplementation with 400 ppm of LCZ resulted in a maximum increase in antioxidant enzymes, indicating that LCZ may affect antioxidant defense system more profoundly than ZnO.


Subject(s)
Catalase , Diet , Glutathione Peroxidase , Glutathione Transferase , Intestine, Small , Intestines , Jejunum , Lipid Peroxidation , Liver , Mucous Membrane , Plasma , RNA, Messenger , Zinc Oxide , Zinc
14.
Clinics ; 73: e16550, 2018. tab, graf
Article in English | LILACS | ID: biblio-952790

ABSTRACT

OBJECTIVES: Consumption of toxic species of mushrooms may have detrimental effects and increase oxidative stress. Paraoxonase, arylesterase and glutathione-S-transferase are antioxidants that resist oxidative stress. In this study, we analyzed the changes in these enzymes during intoxication due to mushrooms. METHODS: The study enrolled 49 adult patients with a diagnosis of mushroom poisoning according to clinical findings and 49 healthy volunteers as the control group. The patients with mild clinical findings were hospitalized due to the possibility that the patient had also eaten the mushrooms and due to clinical findings in the late period, which could be fatal. Paraoxonase, arylesterase, and glutathione-S-transferase concentrations, as well as total antioxidant and oxidant status, were determined in the 49 patients and 49 healthy volunteers by taking blood samples in the emergency department. RESULTS: While paraoxonase, arylesterase, and total antioxidant status were significantly decreased in the patient group (p<0.05), glutathione-S-transferase, total oxidant status and the oxidative stress index were significantly higher (p<0.05). There was a positive correlation between the hospitalization time and the oxidative stress index (r=0.752, p<0.001), whereas a negative correlation was found with glutathione-S-transferase (r=-0.420, p=0.003). CONCLUSION: We observed a significant decrease in paraoxonase and arylesterase and an increase in glutathione-S-transferase and oxidative stress indexes in patients with mushroom poisoning, indicating that these patients had an oxidative status. In particular, a low total antioxidant status and high oxidative stress index may gain importance in terms of the assessment of hospitalization duration.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Carboxylic Ester Hydrolases/blood , Mushroom Poisoning/enzymology , Mushroom Poisoning/blood , Oxidative Stress , Aryldialkylphosphatase/blood , Glutathione Transferase/blood , Reference Values , Spectrophotometry , Case-Control Studies , Statistics, Nonparametric , Length of Stay/statistics & numerical data , Antioxidants/analysis
15.
Salud pública Méx ; 59(5): 540-547, Sep.-Oct. 2017. tab
Article in English | LILACS | ID: biblio-903806

ABSTRACT

Abstract: Objective: To evaluate if variants in the genes CYP1A1 (T3801C and A4889G), CYP1B1 (G119T), GSTM1 (indel) and GSTT1 (indel) are associated with breast cancer (BC) among Mexican women. Materials and methods: 952 incident cases with histologically confirmed BC were matched by age (± 5 years) and zone of residence with 998 healthy population controls. Genetic variants in genes CYP1A1, CYP1B1, GSTM1 and GSTT1were genotyped by allelic discrimination and multiplex PCR. In a subsample of women, 105 markers for ancestry were determined. Results: An increased BC risk, independent of other BC risk factors, was observed among carriers of CYP1B1 G119T genotype (T/T vs. G/G: OR=1.9; 95%CI 1.4-2.5). Conclusion: Our results support the existence of genetic susceptibility for BC conferred by CYP1B1 G119T variant among Mexican women.


Resumen: Objetivo: Evaluar si las variantes en los genes CYP1A1 (T3801C y A4889G), CYP1B1 (G119T), GSTM1 (indel) yGSTT1 (indel), se asocian con el cáncer de mama (CM) en mujeres mexicanas. Material y métodos: Se parearon por edad (± 5 años) y zona de residencia 952 casos incidentes de CM histológicamente confirmado con 998 controles sanos poblacionales. Se genotipificaron variantes en los genes CYP1A1, CYP1B1, GSTM1 y GSTT1 por discriminación alélica y PCR multiplex. En una submuestra de mujeres, se determinaron 105 marcadores de ancestría. Resultados: Se observó un aumento del riesgo de CM, independiente de otros factores de riesgo, entre las portadoras del genotipo CYP1B1 G119T (T/T vs. G/G: RM=1.9; 95%CI 1.4-2.5). Conclusiones: Nuestros resultados soportan la existencia de susceptibilidad genética para CM conferida por la variante CYP1B1 G119T en mujeres mexicanas.


Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Aged , Young Adult , Breast Neoplasms/genetics , Cytochrome P-450 CYP1A1/genetics , Polymorphism, Single Nucleotide , INDEL Mutation , Cytochrome P-450 CYP1B1/genetics , Glutathione Transferase/genetics , Breast Neoplasms/epidemiology , Case-Control Studies , Risk , Africa/ethnology , Multiplex Polymerase Chain Reaction , Mexico/epidemiology
16.
Rev. cuba. obstet. ginecol ; 43(3): 163-172, jul.-set. 2017.
Article in Spanish | LILACS, CUMED | ID: biblio-901324

ABSTRACT

Introducción: entre los factores de riesgo que favorecen la aparición de las lesiones cérvico uterinas, se encuentran la infección por virus de papiloma humano, la promiscuidad, el uso de anticonceptivos orales y el hábito de fumar. No obstante, varias investigaciones refieren que los polimorfismos genéticos podrían contribuir al desarrollo y progresión del cáncer cérvico uterino. Objetivo: identificar en la bibliografía revisada, la frecuencia de asociación de los polimorfismos de Glutation s - transferasa con el cáncer cérvico uterino y con factores de riesgo que inciden en la patología. Métodos: se realizó una extensa revisión de la literatura especializada a través de los buscadores en base de datos de PubMed, EBSCO, NCBI y BVS. Resultados: se constató la variabilidad en los reportes de las frecuencias alélicas de los genotipos GSTM1 y T1 en distintas poblaciones. Se corroboró en varios estudios revisados el hallazgo de asociación entre los genotipos GSTM1 y T1 nulos y cáncer cérvico uterino y, de igual forma con el consumo de tabaco y anticonceptivos orales por tiempo prolongado. Conclusiones: la bibliografía sobre el tema pone en evidencia que los genes que codifican la enzima Glutation s - transferasa intervienen en la protección celular contra los efectos citotóxicos, de manera que cuando éstos presentan alteración se afecta la actividad enzimática, lo que predispone a una mayor susceptibilidad al cáncer(AU)


Introduction: Among risk factors that lead to uterine cervix lesions we can find the human papilloma virus infection, promiscuity, use of oral contraceptive and smoking habit. However, several researches refer that genetic polymorphism could be related to the development and progression of the uterine cervix cancer. Objective: Identify the association of glutathione S- transferases polymorphism with uterine cervix cancer and risk factors relate with this disease, in the revise bibliography. Method: An extensive review was made of the specialized literature using web search in database PubMed, EBSCO, NCBI and BVS. Result: The variability of the allelic frequency of the GSTM1 and T1 genotypes in different populations was confirmed. Besides the association between null GSTM1 and T1 with uterine cervix cancer was corroborated. In addition, this association with smoking habit and the use of oral contraceptive for long time was corroborated. Conclusions: Consulted bibliography shows that genes encoding glutahione S- transferases enzyme contribute to the cellular protection against cytotoxic effects, therefore, alterations in these genes affect the enzymatic activity lead to a major susceptibility to suffer cancer(AU)


Subject(s)
Humans , Female , Polymorphism, Genetic , Uterine Cervical Neoplasms/complications , Glutathione Transferase , Papillomaviridae/genetics , Bibliography of Medicine , Environmental Exposure/adverse effects , Environmental Pollution
17.
Acta cir. bras ; 32(8): 633-640, Aug. 2017. graf
Article in English | LILACS | ID: biblio-886223

ABSTRACT

Abstract Purpose: To evaluate the effect of hyperin in cisplatin-induced liver injury in mice. Methods: Mice were pretreated with hyperin at doses of 25 mg/kg and 50 mg/kg, respectively, for six days, and intraperitoneal injection of cisplatin (40 mg/kg) was administrated one hour after the final intragastrication of hyperin. Twenty-four hours later, blood and liver were collected for further research. Results: A single injection of cisplatin (40 mg/kg) for 24 h significantly increased serum alanine and aspartate aminotransferases (ALT/AST) and gamma glutamyl transferase (GGT) activities, whileas hyperin reversed cisplatin-induced such increases. Liver histopathological examination further demonstrated the protection of hyperin against cisplatin-induced liver injury. Further results showed hyperin reversed cisplatin-induced the increase in content of malondialdehyde (MDA) and the decrease in level of total antioxidant capacity (T-AOC) in liver. Moreover, hyperin increased the levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-s transferase (GST) in cisplatin-induced liver. Conclusion: Hyperin inhibits cisplatin-induced hepatic oxidative stress, which contributes greatly to the amelioration of cisplatin-induced liver injury in mice.


Subject(s)
Animals , Male , Quercetin/analogs & derivatives , Aspartate Aminotransferases/metabolism , Cisplatin/adverse effects , Chemical and Drug Induced Liver Injury/prevention & control , Antineoplastic Agents/adverse effects , Antioxidants/pharmacology , Quercetin/therapeutic use , Quercetin/pharmacology , Reference Values , Lipid Peroxidation , Catalase/analysis , Random Allocation , Reproducibility of Results , Cisplatin/antagonists & inhibitors , Oxidative Stress/drug effects , Alanine Transaminase/metabolism , Chemical and Drug Induced Liver Injury/pathology , Glutathione/analysis , Glutathione Peroxidase/analysis , Glutathione Transferase/analysis , Liver/drug effects , Liver/enzymology , Liver/pathology , Malondialdehyde/analysis , Mice, Inbred ICR , Antioxidants/therapeutic use
18.
Arq. gastroenterol ; 54(2): 101-108, Apr.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-838831

ABSTRACT

ABSTRACT BACKGROUND Gastric cancer is the fourth most common cancer in men and the sixth among women, except for non-melanoma skin tumors, in Brazil. Epidemiological evidences reveal the multifactorial etiology of this cancer, highlighting risk factors such as: infection by the bacterium Helicobacter pylori, advanced age, smoking, chronic alcohol abuse, eating habits and genetic polymorphisms. Considering the context of genetic polymorphisms, there is the absence of the GSTM1 gene. The lack of GSTM1 function to detoxify xenobiotics and promote defense against oxidative stress leads to increased DNA damage, promoting gastric carcinogenesis. This process is multifactorial and the development of gastric cancer results from a complex interaction of these variables. OBJECTIVE The aim of this study was to investigate the association of GSTM1 null polymorphism in the pathogenesis of gastric cancer. METHODS A meta-analysis was conducted from 70 articles collected in SciELO and PubMed databases, between September 2015 and July 2016. In order to evaluate a possible association, we used the odds ratio (OR) and confidence interval of 95% (CI 95%). To assess the heterogeneity of the studies was used the chi-square test. Statistical analysis was performed using the BioEstat® 5.3. RESULTS This study included 70 studies of case-control, including 28,549 individuals, which were assessed for the null polymorphism of the GSTM1 gene, and of which 11,208 (39.26%) were cases and 17,341 (60.74%) were controls. The final analysis showed that the presence of the GSTM1 gene acts as a protective factor against the development of gastric cancer (OR=0.788; 95%CI 0.725-0.857; P<0.0001). Positive statistical association was found in Asia (OR=0.736; 95%CI 0.670-0.809; P<0.0001) and Eurasia (OR=0.671; 95%CI 0.456-0.988; P=0.05). However, statistically significant data was not obtained in Europe (OR=1.033; 95%CI 0.873-1.222; P=0.705) and America (OR=0.866; 95%CI 0.549-1.364; P=0.534). Therefore, the results can not be deduced around the world. CONCLUSION This meta-analysis concluded that the presence of the GSTM1 gene is a protector for the emergence of gastric cancer, especially in Asian countries, but this result was not found in Europe and America.


RESUMO CONTEXTO No Brasil, o câncer gástrico é o quarto mais comum em homens e o sexto entre as mulheres, excetuando-se os tumores de pele não melanoma. Aspectos epidemiológicos evidenciam a etiologia multifatorial desta neoplasia, destacando como fatores de risco: a infecção pela bactéria Helicobacter pylori, idade avançada, tabagismo, etilismo crônico, hábitos alimentares e polimorfismos genéticos. No contexto dos polimorfismos genéticos, tem-se a ausência do gene GSTM1. A falta da função de GSTM1 em detoxificar xenobióticos e promover defesa contra o estresse oxidativo, leva ao maior dano do DNA, favorecendo a carcinogênese gástrica. Este processo é multifatorial e o desenvolvimento do câncer gástrico resulta de uma interação complexa dessas variáveis. ObJETIVO O objetivo do presente estudo foi investigar a associação do polimorfismo nulo de GSTM1 na gênese do câncer gástrico. MÉTODOS Foi conduzida uma meta-análise a partir de 70 artigos colhidos dos bancos de dados: SciELO e PubMed, entre setembro de 2015 e julho de 2016. Para avaliar uma possível associação, utilizou-se o odds ratio (OR) e intervalo de confiança de 95% (IC 95%). Para avaliar a heterogeneidade dos estudos, utilizou-se o teste do qui-quadrado. A análise estatística foi realizada utilizando-se o BioEstat® 5.3. RESULTADOS A presente pesquisa contou com 70 estudos do tipo caso-controle que incluíram 28.549 indivíduos avaliados para o polimorfismo nulo do gene GSTM1, dos quais 11.208 (39,26%) eram casos e 17.341 (60,74%) eram controles. A análise final mostra que a presença do gene GSTM1 funciona como um fator de proteção contra o desenvolvimento de câncer gástrico (OR=0,788; IC95% 0,725-0,857; P<0,0001). Associação estatística positiva foi encontrada na Ásia (OR=0,736; IC95% 0,670-0,809; P<0,0001) e Eurásia (OR=0,671; IC95% 0,456-0,988; P=0,05). No entanto, não temos dados com significância estatística da Europa (OR=1,033; IC95% 0,873-1,222; P=0,705) e América (OR=0,866; IC95% 0,549-1,364; P=0,534) para inferir proteção ao câncer gástrico no mundo. CONCLUSÃO Esta meta-análise, conclui que a presença do gene GSTM1 é protetora para o surgimento do câncer gástrico, principalmente nos países asiáticos, porém tal resultado não foi encontrado se comparado isoladamente os estudos realizados na Europa e na América.


Subject(s)
Humans , Male , Female , Genetic Predisposition to Disease/genetics , Glutathione Transferase/genetics , Neoplasms/genetics , Polymorphism, Genetic , Risk Factors , Genotype
19.
Bol. latinoam. Caribe plantas med. aromát ; 16(2): 88-98, mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-881315

ABSTRACT

Inflammation is a cellular defensive mechanism associated to oxidative stress. The administration of nitrofurantoin, nifurtimox and acetaminophen generates oxidative stress by their biotransformation through CYP450 system. The main adverse effect described for the first two drugs is gastrointestinal inflammation and that of the last, hepatitis. Therefore, standardised dry extracts from Rosmarinus officinalis, Buddleja globosa Hope, Cynara scolymus L., Echinacea purpurea and Hedera helix were tested to evaluate their capacity to decrease drug-induced oxidative stress. For that, rat liver microsomes were incubated with drugs in the presence of NADPH (specific CYP450 system cofactor) to test oxidative damage on microsomal lipids, thiols, and GST activity. All drugs tested induced oxidation of microsomal lipids and thiols, and inhibition of GST activity. Herbal extracts prevented these phenomena in different extension. These results show that antioxidant phytodrugs previously evaluated could alleviate drugs adverse effects associated to oxidative stress.


Inflamación es un mecanismo de defensa el cual está asociado a estrés oxidativo. La administración de nitrofurantoína, nifurtimox y paracetamol genera estrés oxidativo al metabolizarse a través del sistema CYP450. El principal efecto adverso de los dos primeros fármacos es inflamación gastrointestinal y del tercero, hepatitis. Por lo tanto, utilizamos diversos extractos herbales para disminuir el estrés oxidativo inducido por estos fármacos. Para esto se incubaron microsomas hepáticos de rata con dichos fármacos en presencia de NADPH (cofactor específico del sistema CYP450) y se evaluó el daño oxidativo generado sobre los lípidos, los tioles y la actividad GST microsómica. Todos los fármacos indujeron oxidación de los lípidos y los tioles microsómicos e inhibieron la actividad GST. Los extractos herbales previnieron estos fenómenos oxidativos en diferente extensión. Estos resultados indican que fitofármacos antioxidantes previamente evaluados, podrían aliviar los efectos adversos asociados a estrés oxidativo de los fármacos.


Subject(s)
Animals , Male , Antioxidants/pharmacology , Microsomes, Liver/drug effects , Oxidative Stress/drug effects , Plant Extracts/pharmacology , Acetaminophen/adverse effects , Glutathione Transferase/metabolism , Lipid Peroxidation , Microsomes, Liver/enzymology , NADP/analysis , Nifurtimox/adverse effects , Nitrofurantoin/adverse effects , Plant Extracts/chemistry , Polyphenols/analysis , Rats, Sprague-Dawley , Sulfhydryl Compounds
20.
National Journal of Andrology ; (12): 367-371, 2017.
Article in Chinese | WPRIM | ID: wpr-812758

ABSTRACT

Approximately 2,300 genes are found to be associated with spermiogenesis and their expressions play important roles in the regulation of spermiogenesis. In recent years, more and more attention has been focused on the studies of the genes associated with oligospermia, asthenospermia and teratospermia and their molecular mechanisms. Some genes, such as GSTM1, DNMT3L, and CYP1A1, have been shown to be potentially associated with oligospermia; some, such as CATSPER1, CRISP2, SEPT4, TCTE3, TEKT4, and DNAH1, with asthenospermia; and still others, such as DPY19L2 and AURKC, with teratospermia. These findings have provided a molecular basis for the studies of the pathogenesis of oligospermia, asthenospermia and teratospermia, as well as a new approach to the exploration of new diagnostic and therapeutic techniques.


Subject(s)
Asthenozoospermia , Genetics , Aurora Kinase C , Genetics , Calcium Channels , Genetics , Cytochrome P-450 CYP1A1 , Genetics , Cytoplasmic Dyneins , DNA (Cytosine-5-)-Methyltransferases , Genetics , Dyneins , Genetics , Glutathione Transferase , Genetics , Glycoproteins , Genetics , Humans , Male , Membrane Proteins , Genetics , Microtubule Proteins , Genetics , Oligospermia , Genetics , Spermatogenesis , Genetics , Teratozoospermia , Genetics
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