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1.
Article in Chinese | WPRIM | ID: wpr-878984

ABSTRACT

One new and two known dammarane-type saponins were isolated from the leaves of Gynostemma pentaphyllum using various chromatographic methods. Their structures were identified by HR-ESI-MS,~( 1)H-NMR, ~(13)C-NMR, 2 D-NMR spectra as 2α,3β,12β,20,24(S)-tetrahdroxydammar-25-en-3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside(1, a new compound, namely gypenoside J5) and 2α,3β,12β,20,24(R)-tetrahdroxydammar-25-en-3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-20-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranoside(2) and 2α,3β,12β,20-tetrahydroxy-25-hydroperoxy-dammar-23-en-3-O-[β-D-glucopyranosyl(1→2)][β-D-glucopyranosyl]-20-O-[β-D-xylopyranosyl(1→6)]-β-D-glucopy-ranoside(3), respectively. Compounds 1 and 2 were a pair of C-24 epimers. All compounds showed weak cytotoxicity agxinst H1299, HepG2, PC-3, SH-SY5 Y cancer cell lines. However, they exerted protective effect against SH-SY5 Y cellular damage induced by H_2O_2 dose-dependently, of which compound 1 displayed the strongest antioxidant effect. The present study suggested that G. pentaphyllum has antioxidative potential and the saponins from G. pentaphyllum are considered as the active compounds with neuroprotecitve effect.


Subject(s)
Gynostemma , Molecular Structure , Neuroprotective Agents/pharmacology , Saponins/pharmacology , Triterpenes/pharmacology
2.
Article in Chinese | WPRIM | ID: wpr-878961

ABSTRACT

The Qinling-Daba Mountains area is the main producing areas of Gynostemma longipes for medicinal usage, and samples of wild whole plants in Pingli, Shaanxi Province and Qingchuan, Sichuan Province were collected. The ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UHPLC-Q-TOF-MS~E) was used to profile the chemical compositions and analyze the similarities and differences of G. longipes samples in these areas. Based on the accurate molecular weight and fragment information obtained from Q-TOF-MS~E, the structures of the main components were identified by combining with the mass spectra, chromatographic behaviors of reference standards and related literatures. The results showed that the components of wild G. longipes from different places among Qinling-Daba Mountains area were similar. Forty-five chemical components were identified in the whole plant of G. longipes from Pingli, Shaanxi Province, including 43 triterpenoid saponins and 2 flavonoids which contain all main peaks in its fingerprint. The main components are dammarane-type triterpenoid saponins, such asgypenoside ⅩLⅨ, gypenoside A and its malonylated product of glycosyl.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Gynostemma , Mass Spectrometry , Saponins
3.
Article in Chinese | WPRIM | ID: wpr-878859

ABSTRACT

In response to no national standard for Gynostemma pentaphyllum, a market survey was carried out, and 17 batches of gypenosides extract and 29 batches of Gypenosides Tablets on the market were collected. With gypenoside A as an index, the TLC qualitative identification and HPLC quantitative evaluation method of gypenosides extract and tablets was established. Based on the determination results of 17 batches of gypenosides extract and 29 batches of Gypenosides Tablets, the quality standards of gypenosides extract and tablets were formulated respectively, so as to give suggestions for improving the quality standards of gypenosides extract and tablets. Compared with the existing ministerial standards, the qualitative identification and quantitative detection of specific components were added, in order to provide scientific basis and suggestions for the revision of the quality standard of gypenosides extract and tablet preparation.


Subject(s)
Gynostemma , Plant Extracts , Reference Standards , Tablets
4.
Article in Chinese | WPRIM | ID: wpr-774571

ABSTRACT

Four flavonoids were isolated from Gynostemma pentaphyllum by chromatography methods and their structures were identified by MS and NMR spectra data as quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-galactopyranoside( 1),quercetin-3-O-( 2″,6″-di-α-L-rhamnosyl)-β-D-glucopyranoside( 2),quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-galactopyranoside( 3),and quercetin-3-O-( 2″-α-L-rhamnosyl)-β-D-glucopyranoside( 4). Among them,compounds 1-3 were obtained from the Cucurbitaceae family for the first time.The four flavonoids showed potent antioxidant effects against the DPPH,·OH and ■radicals in vitro,especially for DPPH radical scavenging activity with the IC50 values of 71. 4,29. 5,48. 3 and 79. 2 μmol·L~(-1),respectively. Moreover,the four flavonoids displayed strong cytoprotection against AAPH-induced oxidative damage in LLC-PK1 cells by suppressing the increase of malondialdehyde( MDA) and the decrease of the superoxide dismutase( SOD) and glutathione( GSH). Since further research is needed to prove its efficacy in vivo and clinical trial,the study may provide four potential antioxidants from G. pentaphyllum.


Subject(s)
Animals , Antioxidants , Flavonoids , Gynostemma , LLC-PK1 Cells , Oxidative Stress , Plant Extracts , Quercetin , Swine
5.
Article in Chinese | WPRIM | ID: wpr-771709

ABSTRACT

To investigate the differences of chemical compositions in Gynostemma pentaphyllum leaves prepared by different processing methods. Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was used to compare the chemical compositions between shade-dried processing and drum-dried processing. Forty six gypenosides were identified by control comparison, liquid chromatography-mass spectrometry(LC-MSn) fragmentation information, and literature data. The mass spectral peak area statistics was combined with principal component analysis(PCA), and the results showed that eight batches of Gynostemma pentaphyllum leaves samples were divided into two groups according to the two different processing methods; ten chemical compositions with significant differences were screened according to mass spectrum information combined with partial least-squares discriminant analysis(PLS-DA). The result showed that most parent nucleus of the gypenosides contained three to four glycosides in drum-dried samples, and one to two glycosides in the shade-dried samples. It was inferred from further MS analysis that desugarization of gypenosides was present to produce secondary glycosides with the effect of glucosidase in the shade-drying, thus resulting in difference in compositions. This study provided data support for harvesting, processing and quality control of Gynostemma pentaphyllum leaves.


Subject(s)
Chromatography, High Pressure Liquid , Gynostemma , Chemistry , Mass Spectrometry , Plant Leaves , Chemistry , Saponins , Chemistry
6.
Article in Chinese | WPRIM | ID: wpr-351284

ABSTRACT

The sequences of ITS, matK, rbcL and psbA-trnH of 9 Gynostemma species or variety including 38 samples were compared and analyzed by molecular phylogeny method. Hemsleya macrosperma was designated as outgroup. The MP and NJ phylogenetic tree of Gynostemma was built based on ITS sequence, the results of PAUP phylogenetic analysis showed the following results: (1) The eight individuals of G. pentaphyllum var. pentaphyllum were not supported as monophyletic in the strict consensus trees and NJ trees. (2) It is suspected whether G. longipes and G. laxum should be classified as the independent species. (3)The classification of subgenus units of Gynostemma plants is supported.


Subject(s)
Gynostemma , Classification , Genetics , Molecular Sequence Data , Phylogeny , Plant Proteins , Genetics , Sequence Analysis, DNA
7.
Article in Chinese | WPRIM | ID: wpr-321379

ABSTRACT

<p><b>OBJECTIVE</b>To explore the feasibility of preparing novel gypenosides long-circulating liposomes with PEG grafted on beta-sitosterol (PEG-Sito).</p><p><b>METHOD</b>Succinicanhydride was adopted to connect beta-sitosterol and PEG 2000. Sphingomyelin and PEG-Sito was used as material to prepare gypenosides long-circulating liposomes by using ethanol injection method. Encapsulation efficiency was determined by using protamine precipitation method. H-NMR was used to verify the synthesis of PEG-Sito, the novel gypenosides long-circulating liposomes were characterized by particle size, zeta potential and atomic force microscope.</p><p><b>RESULT</b>The synthesis of PEG-Sito was verified by 1H-NMR. Encapsulation efficiency of long-circulating liposomes prepared by ethanol injection method was 74.3%, particle size was 288.1 nm, zeta potential was -20.25 mV, the morphology were round observed by AFM.</p><p><b>CONCLUSION</b>The novel gypenosides long-circulating liposomes prepared with PEG-Sito was feasible, it had a high encapsulation efficiency and good morphology.</p>


Subject(s)
Drug Compounding , Methods , Feasibility Studies , Gynostemma , Chemistry , Liposomes , Blood , Chemistry , Plant Extracts , Chemistry , Polyethylene Glycols , Chemistry , Reproducibility of Results , Sitosterols , Chemistry , Sphingomyelins , Chemistry
8.
Article in Chinese | WPRIM | ID: wpr-319686

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the feasibility of preparing gypenosides liposomes with sphingomyelin and cholesterol, and optimize the preparation process and prescription.</p><p><b>METHOD</b>Gypenosides liposomes were prepared with sphingomyelin and cholesterol. The entrapment efficiency was determined by the protamine precipitation method. The entrapment efficiency was taken as the evaluation index to screen out the optimum preparation process for the new-type gypenosides liposomes. The preparation process was optimized by the orthogonal design. The new-type gypenosides liposomes were characterized by grain size, potential and atomic force microscope (AFM).</p><p><b>RESULT</b>Ethanol injection was the optimum process to prepare gypenosides liposome with sphingomyelin and cholesterol as follow: the ratio of gypenosides to sphingomyelin was 1: 10, the ratio of sphingomyelin to cholesterol was 4: 1, the pH of PBS buffer solution was 7.0, the hydration temperature was 45 degrees C, the entrapment efficiency was 79.06%, particle size was 191.4 nm, the Zeta potential was -33.16 mV, and the morphology were round under AFM.</p><p><b>CONCLUSION</b>It was feasible to prepare gypenosides liposome with sphingomyelin and cholesterol. The gypenosides liposomes prepared by the optimum preparation process were good in morphology, particle size and reproducibility.</p>


Subject(s)
Cholesterol , Chemistry , Drug Carriers , Chemistry , Drug Compounding , Methods , Gynostemma , Chemistry , Liposomes , Chemistry , Particle Size , Plant Extracts , Chemistry , Sphingomyelins , Chemistry
9.
Article in Chinese | WPRIM | ID: wpr-274315

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of gypenosides on DMN-induced liver fibrosis in rats.</p><p><b>METHOD</b>A rat liver fibrosis model was established by injecting DMN intraperitoneally. Four weeks later, model rats were randomly devided into three groups: the model group, the gypenosides treated group (200 mg x kg(-1)) and the colchicine treated group (0.1 mg x kg(-1)), with 10 specimens for each group. After a 2-week treatment, following parameters were observed: (1) last body weight, weight ratio between liver and spleen; (2) content of liver hydroxyproline (Hyp); (3) activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyltransferase (gamma-GT), content of albumin (Alb) and total bilirubin( TBiL) in serum; (4) liver pathology (Sirius red staining and HE staining); (5) activity of liver superoxide dismutase (SOD), glutathione reduced (GSH), glutathione peroxidase (GSH-Px) and content of liver maleic dialdehyde (MDA).</p><p><b>RESULT</b>There were classic liver cirrhosis pathological changes in model groups. Compared with the normal group, liver Hyp content, activity of serum ATL, AST, gamma-GT and content of serum TBiL, MDA of model groups significantly increased; content of serum Alb and liver GSH, activity of liver SOD and GSH-Px decreased significantly in model groups. In comparison with the model group, liver cirrhosis remarkable improved in the gypenosides group, content of liver Hyp reduced significantly (P < 0.01), which was equal to the colchicine group. Compared with the model group, liver function parameters improved markedly in the gypenosides group; liver SOD and GSH-Px activities significantly increased; MDA content reduced significantly (P < 0.05).</p><p><b>CONCLUSION</b>Gypenosides shows an effect in treating DMN-induced liver fibrosis in rats.</p>


Subject(s)
Animals , Body Weight , Dimethylnitrosamine , Glutathione , Metabolism , Glutathione Peroxidase , Metabolism , Gynostemma , Hydroxyproline , Metabolism , Liver , Metabolism , Pathology , Liver Cirrhosis , Drug Therapy , Metabolism , Pathology , Male , Malondialdehyde , Metabolism , Organ Size , Plant Extracts , Pharmacology , Therapeutic Uses , Rats , Rats, Wistar
10.
Article in Chinese | WPRIM | ID: wpr-231589

ABSTRACT

<p><b>OBJECTIVE</b>To explore the method and significance for studying active anti-liver fibrosis ingredients consisted Chinese medicine compound prescription based on Chinese medicine theory.</p><p><b>METHODS</b>Optimized prescription was screened out, adopting uniform block design with 4-factor 8-level table and regression analysis, through applying the four known effective ingredients (cordyceps sinensis polysaccharide, salvianolic acid B, amygdaloside and gypenosides) of Fuzheng Huayu Capsule (FZHYC, a new Chinese medine anti-liver fibrosis drug) to two rat liver fibrosis models established separately by dimethylnitrosamine (DMN) and CCl4, and taking the liver content of hydroxyproline (Hyp) as the screen index. Then a further study for comparing and verifying the efficacy of the obtained optimized prescription was conducted on the two former models respectively by observing the changes of Hyp content in liver, serum ALT activity and fibrosis pathology after medication, controlled by the original FZHYC and the recipe assembled by all the four ingredients.</p><p><b>RESULTS</b>Two optimized prescriptions (OPA and OPB) were screened out separately in the studies conducted on the two models. Both of them were consisted of cordyceps sinensis polysaccharide, Amygdaloside and Gypenosides, but different in constituent ratio, i.e., the ratio in OPA was 60 : 80: 50, and that in OPB, 20: 160: 50. Verifying study showed both OPA and OPA were significantly effective, with the efficacy equivalent to that of FZHYC (P>0.05). However, when they were used in combining with salvianolic acid B (the cutout ingredient in the screening), the efficacy lowered surely.</p><p><b>CONCLUSIONS</b>Uniform design is a valuable method in the compatibility research of Chinese Medicine drugs' composition. To assemble a new compound recipe reasonably based on the prescription of traditional compound recipe could make its effect equivalent to that of the original prescription. Ingredients or constituents in a prescription, either presented synergistic or antagonistic effects, are not randomly stacked together, and they should be orderly assembled in intrinsic rules of qualitative and quantitative changing.</p>


Subject(s)
Amygdalin , Animals , Drug Combinations , Drug Design , Drugs, Chinese Herbal , Therapeutic Uses , Gynostemma , Liver Cirrhosis , Drug Therapy , Male , Plant Extracts , Rats , Rats, Wistar , Regression Analysis
11.
Article in Chinese | WPRIM | ID: wpr-231512

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect and possible mechanism of gypenoside (GP) on expression of inflammatory factors in aortic lesion of rats with high-fat induced atherosclerosis.</p><p><b>METHODS</b>Atherosclerotic rat model was established by feeding high-fat diet and intraperitoneal injection of vitamin D3. Sixty healthy male SD rats were randomly divided into the normal group, the model group, the simvastatin treated group and the three GP groups treated respectively with different dosages of GP. Rats were sacrificed 7 weeks later, their histopathological changes in thoracic aorta were observed by light microscope; expressions of intercellular adhesion molecule 1 (ICAM-1), monocyte chemotactic protein-1 (MCP-1) and nuclear factor-kappaBp65 (NF-kappaBp65) in aortic wall were detected by immunohistochemistry; serum level of oxidized low-density lipoprotein (ox-LDL) was determined by ELISA; serum total antioxidant capacity determined by colorimetry, and serum malondialdehyde (MDA) level determined by Thiobarbituric acid method.</p><p><b>RESULTS</b>In comparing with the model group, GPS showed actions in lessening the atherosclerosis lesion; reducing expressions of ICAM-1, MCP-1 and NF-kappaBp65 in aortic wall (P<0.01) and serum levels of MDA, ox-LDL (P < 0.01), as well as increasing the serum level of total antioxidant capacity (P < 0.01 ).</p><p><b>CONCLUSION</b>GP can down-regulate the expressions of ICAM-1 and MCP-1, inhibit the atherosclerosis formation in experimental rats, its mechanism might be related with its anti-oxidation effect and further inhibiting on the NF-kappaB activation.</p>


Subject(s)
Animals , Atherosclerosis , Metabolism , Pathology , Chemokine CCL2 , Metabolism , Gynostemma , Inflammation , Intercellular Adhesion Molecule-1 , Metabolism , Male , NF-kappa B , Metabolism , Oxidative Stress , Plant Extracts , Pharmacology , Rats , Rats, Sprague-Dawley
12.
Article in Chinese | WPRIM | ID: wpr-283774

ABSTRACT

<p><b>OBJECTIVE</b>To establish a convenient and effective method for the identification of Gynostemma and Cayratia japonica.</p><p><b>METHOD</b>Eight species, including Gynostemm pentaphyllum, G. pentagynum, G. cardiospermum, G. longipe, G. yixingense, G. laxiflorum, G. guangxiense and C. japonica were investigated through PCR - RFLP of six chloroplast DNA fragments. The six gene fragments were digested by six restriction endonuclease respectively, including Taq I, Hpa II, EcoR I, Rsa I, Hha I, Hind III.</p><p><b>RESULT</b>Seven species of Gynostemma and their adulterant could be identified by trnK1f-trnK2r and Rsa.</p><p><b>CONCLUSION</b>PCR - RFLP provides a quick, reliable molecular marker technique for identification of Cynostemma and their adulterant Cayratia japonica.</p>


Subject(s)
DNA, Chloroplast , Genetics , DNA, Plant , Genetics , Gynostemma , Classification , Genetics , Polymerase Chain Reaction , Methods , Polymorphism, Restriction Fragment Length , Genetics , Vitaceae , Classification , Genetics
13.
Article in Chinese | WPRIM | ID: wpr-310765

ABSTRACT

<p><b>AIM</b>To study the regulation mechanism of GP on plasma lipoprotein metabolism and To explore its mechanism of anti-lipoperoxidation in the experimental hyperglycemia rats.</p><p><b>METHODS</b>The rats were raised with high fat diet for six weeks,and the model of hyperglycemia was then established. After that, those rats were treated with high or low dose of GP and xuezhikang as a masculine comparison for four weeks. Then, those rat were executed, and detected the plasma TC, TG, LDL-C, HDL-C, GSH-Px, at the same time the SOD, CAT and MDA concentration also be mensurated.</p><p><b>RESULTS</b>The results showed that high and low dose of GP could decrease the concentration of serum LDL-C, cholesterol and triglyceride remarkably and raise the concentration of HDL-C. The activity of GSH-Px, SOD and CAT in GP groups were promoted and the level of MDA was decreased distinctly.</p><p><b>CONCLUSION</b>The GP can therapy the abnormity of serum lipid and has obviously anti-lipoperoxidation affection.</p>


Subject(s)
Animals , Cholesterol , Blood , Cholesterol, LDL , Blood , Gynostemma , Hyperlipidemias , Drug Therapy , Metabolism , Lipid Peroxidation , Lipids , Blood , Lipoproteins, HDL , Blood , Male , Phytotherapy , Plant Extracts , Therapeutic Uses , Rats , Rats, Wistar , Triglycerides , Blood
14.
Article in English | WPRIM | ID: wpr-282427

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of gypenoside (Gyp) on the activity of microsomal Na(+), K(+)-ATPase in rat's heart and brain in vitro.</p><p><b>METHODS</b>The microsomal Na(+), K(+)-ATPase was prepared from rat's heart and brain by differential centrifugation. The activity of microsomal Na(+), K(+)-ATPase was assayed by colorimetric technique. Enzyme kinetic analysis method was used to analyze the effect of Gyp on the microsomal Na(+), K(+)-ATPase of rats.</p><p><b>RESULTS</b>Gyp reversibly inhibited the brain and heart's microsomal Na(+), K(+)-ATPase in a concentration-dependent manner, and showed a more potent effect on enzyme in the brain. The IC(50) of Gyp for the heart and brain were 58.79+/-8.05 mg/L and 52.07+/-6.25 mg/L, respectively. The inhibition was enhanced by lowering the Na(+), or K(+) concentrations or increasing the ATP concentration. Enzyme kinetic studies indicated that the inhibitory effect of Gyp on the enzyme is like that of competitive antagonist of Na(+), the counter-competitive inhibitor for the substrate ATP, and the mixed-type inhibitor for K(+).</p><p><b>CONCLUSION</b>Gyp displays its cardiotonic and central inhibitory effects by way of inhibiting heart and brain's microsomal Na(+), K(+)-ATPase activities in rats.</p>


Subject(s)
Adenosine Triphosphate , Pharmacology , Animals , Brain , Enzyme Inhibitors , Pharmacology , Gynostemma , Kinetics , Male , Myocardium , Plant Extracts , Pharmacology , Rats , Rats, Wistar , Sodium-Potassium-Exchanging ATPase
15.
Article in Chinese | WPRIM | ID: wpr-307557

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of total flaveos of Gymostemma pentaphyllum on the protein expression of apoptosis-associated Fas/FasL gene and tumor necrosis factor-alpha (TNF-alpha) concentration in cultured neonatal rat cardiomyocytes with hypoxia-reoxygenation (H/R).</p><p><b>METHOD</b>A cultured primary neonatal rat cardiomyocytes model with H/R was erected, experiments were divided into six groups, (1)control group, (2)H/R group, (3)15 mg x L(-1) TFG plus H/R group, (4)45 mg x L(-1) TFG plus H/R group, (5) 105 mg x L(-1) TFG plus H/R group, (6)105 mg x L(-1) TFG group. TNF-aconcentration in cultured cardiomyocytes with H/R, was determined by ELISA method, the protein expression of Fas/FasL genes were estimated by immunohisto-chemistry.</p><p><b>RESULT</b>After cardiomyocytes were made with H/R, Compared with control group, the positive expression index (PEI) of Fas/FasL proteins in cardiomyocytes increased significantly, Compared with H/R groups, the PEI of Fas/FasL proteins were lower significantly in groups with different dosages of TFG (P < 0.05). TFG inhibited the secretion of TNF-alpha from myocardial cells and increased the survival rate of myocardial cells.</p><p><b>CONCLUSION</b>The protein expression of apoptosis-associated Fas/FasL genes increased during H/R. The TFG can protect myocardium against H/R injury by decreasing the production of TNF-alpha, downregulating the protein expression of Fas/FasL genes, and then inhibiting myocyte apoptosis.</p>


Subject(s)
Animals , Animals, Newborn , Apoptosis , Cell Hypoxia , Cells, Cultured , Drugs, Chinese Herbal , Pharmacology , Enzyme-Linked Immunosorbent Assay , Fas Ligand Protein , Metabolism , Flavones , Pharmacology , Gynostemma , Chemistry , Immunohistochemistry , Myocytes, Cardiac , Cell Biology , Metabolism , Oxygen Consumption , Physiology , Plants, Medicinal , Chemistry , Rats , Rats, Sprague-Dawley , Tumor Necrosis Factor-alpha , Metabolism
16.
Article in Chinese | WPRIM | ID: wpr-358122

ABSTRACT

<p><b>OBJECTIVE</b>To review the species and distribution of the medicinal plants peculiar to Guizhou and provide evidence for application, protection and collection.</p><p><b>METHOD</b>Open-air investigation, data collection and specimen identification.</p><p><b>RESULT</b>More than eighty kinds of the medical plants peculiar to Guizhou have been identified.</p><p><b>CONCLUSION</b>Guizhou has a diversity of medicinal plants. The area of distribution of most species is restricted and the population is small. Some of the species have higher medicinal and scientific research values.</p>


Subject(s)
Berberis , Classification , China , Conservation of Natural Resources , Epimedium , Classification , Gynostemma , Classification , Pharmacognosy , Plants, Medicinal , Classification
17.
Article in Chinese | WPRIM | ID: wpr-358111

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of Qilan Tangzhining capsule on glucose and lipid metabolism in diabetes mellitus and hyperlipemia.</p><p><b>METHOD</b>Rats were fed with high sugar and fat food for 2 months, then 2% streptozocin (STZ, 30 mg kg(-1)) was injected intraperitoneally to induce hyperglycemia and hyperlipemia. After the model rats were administrated drugs, the blood glucose, the serum lipids, and the histopathology of the liver and pancreas were observed.</p><p><b>RESULT</b>Qilan Tangzhining capsule could decrease the FBG and the serum TC, TG, LDL-C, increase HDL-C, and improve the histopathologic injury of the liver and pancreas.</p><p><b>CONCLUSION</b>Qilan Tangzhining capsule can not only adjust the blood glucose and lipid, but also improve the histopathology injury of the liver and pancreas.</p>


Subject(s)
Animals , Astragalus propinquus , Chemistry , Blood Glucose , Metabolism , Capsules , Diabetes Mellitus, Experimental , Blood , Drug Combinations , Drugs, Chinese Herbal , Pharmacology , Gynostemma , Chemistry , Hyperlipidemias , Blood , Lipids , Blood , Male , Plants, Medicinal , Chemistry , Random Allocation , Rats , Rats, Wistar
18.
Article in Chinese | WPRIM | ID: wpr-272789

ABSTRACT

<p><b>OBJECTIVE</b>To distinguish female and male plants of Gynostemma pentaphyllum quickly and accurately in the stage of seedling and to understand the relation between sex differentiation and endogenous plant hormone.</p><p><b>METHOD</b>Stained methods of BTB and enzyme linked immuno-sorbent Assay were used.</p><p><b>RESULT</b>10 Hours after being dyed, the liquid extract of female plants turned into yellow, while extract of male ones turned green. There were obvious differences on iPAs and GA(1 + 3) contents but no differences on IAA and ABA contents between male and female plants during the stages of seedling and vegetative growth.</p><p><b>CONCLUSION</b>BTB can be used for distinguishing female and male plants and there were a certain relation between sex and endogenous plant hormones.</p>


Subject(s)
Gibberellins , Metabolism , Gynostemma , Metabolism , Plant Growth Regulators , Metabolism , Plant Leaves , Metabolism , Plants, Medicinal , Metabolism , Sex Factors
19.
Article in Chinese | WPRIM | ID: wpr-256365

ABSTRACT

<p><b>OBJECTIVE</b>To provide foundation for developing Gynostemma pentaphyllum with physic and edible use.</p><p><b>METHOD</b>To plant the seven leaves with sweet taste of Enshi, Five leaves with honey taste of Enshi, Makino (picric taste) taste, in the same ecological environment in accordance with the require of mathematical statistics and give the same management, At the same time, sowing and reaping dry grass to do composition analysis;</p><p><b>RESULT</b>The ginseng total saponin, the total amino acid. VitE, VitB1, VitB2 of the two new variety are 33% and 34.3%, 11.8% and 4.7%, 76% and 45.8%, 46% and 76.1%, 41.2% and 25% higher than the raw variety of Gynostemma pentaphyllam Makino respectively. All of them achieve the remarkable standard. The total sugar of the two is 16.3% and 4.4% lower than the raw variety of G. pentaphyllum respectively.</p><p><b>CONCLUSION</b>The two new variety is not only with the distinct flavor and good taste, but also has wider used domain More amount of utilization, Better medical treatment and health care than the raw variety of G. pentaphyllum. It is more worthful to develop in industry domain.</p>


Subject(s)
Amino Acids , Carbohydrates , Genetic Variation , Ginsenosides , Gynostemma , Chemistry , Genetics , Plants, Edible , Chemistry , Genetics , Plants, Medicinal , Chemistry , Genetics , Riboflavin , Thiamine , Vitamin E
20.
Pharmaceutical Journal ; : 10-12, 1999.
Article in Vietnamese | WPRIM | ID: wpr-1664

ABSTRACT

The acute toxicity of gynostema pentaphyllum (Thunb) makino has been tested in mice at the dose 50g/per kg of body weight and the semi acute toxicity have been tested in rabbit. The results of experiment showed that the plant was not toxic and could be used for a long time (1 month) safely without any effect on biochemical and haematological indices


Subject(s)
Gynostemma , Pharmaceutical Preparations , Therapeutics
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