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1.
Mem. Inst. Oswaldo Cruz ; 112(3): 220-223, Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-841773

ABSTRACT

The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.


Subject(s)
Humans , Herpesvirus 2, Human/genetics , Herpesvirus 1, Human/genetics , Herpes Simplex/virology , DNA, Viral/genetics , Sensitivity and Specificity , Viral Load , Real-Time Polymerase Chain Reaction , Herpes Simplex/diagnosis
3.
Article in English | WPRIM | ID: wpr-213114

ABSTRACT

PURPOSE: To comparatively analyze the methodological efficacy of the polymerase chain reaction (PCR) assay for herpes simplex virus 1 (HSV) detection in tears. METHODS: This retrospective study reviewed the medical records of 115 patients who were clinically diagnosed with herpes keratitis, and their tear samples were collected for HSV detection. PCR positive rates were analyzed for their dependence on the PCR primers used (conventional PCR primer vs. nested PCR primer), the tear collecting method used (micropipetting vs. collection with schirmer strip), the disease manifestation and the patient's previous medication history. RESULTS: HSV DNA was detected in 23 out of 115 (20%) tear samples. The PCR positive rate in tear samples did not differ depending on the PCR primer or tear collection method used. Typical epithelial lesions showed a higher positive rate (31.4%) than atypical epithelial lesions (10.9%). The previous history of the antiviral agent seemed to affect the PCR positive rate. CONCLUSIONS: Although the PCR positive rate was not dependent on the tear collection method or primers, HSV detection in tears using PCR was shown to be a supplementary diagnostic test in typical and atypical herpes epithelitis.


Subject(s)
DNA, Viral/analysis , Epithelium, Corneal/virology , Female , Follow-Up Studies , Herpesvirus 1, Human/genetics , Humans , Keratitis, Herpetic/diagnosis , Male , Middle Aged , Polymerase Chain Reaction/methods , Reproducibility of Results , Retrospective Studies , Tears/virology
4.
Article in English | WPRIM | ID: wpr-13091

ABSTRACT

Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for treating infectious bovine rhinotracheitis disease.


Subject(s)
Animals , Antibodies, Viral/blood , Blotting, Western/veterinary , Cattle , Female , Genetic Vectors/immunology , Herpesvirus 1, Bovine/genetics , Herpesvirus 1, Human/genetics , Immunity, Humoral/immunology , Immunization/methods , Infectious Bovine Rhinotracheitis/immunology , Mice , Mice, Inbred BALB C , Neutralization Tests/veterinary , Specific Pathogen-Free Organisms , Viral Proteins/genetics , Viral Vaccines/immunology
5.
Mem. Inst. Oswaldo Cruz ; 104(3): 399-410, May 2009. ilus
Article in English | LILACS | ID: lil-517020

ABSTRACT

Amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. As the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. In addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 Kbp of foreign DNA, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. We present here recent technological developments that have significantly improved and extended the use of amplicons, both in cultured cells and in living organisms. In addition, this review also discusses the many difficulties still pending to be solved, in order to achieve stable and physiologically regulated transgene expression.


Subject(s)
Animals , Humans , Gene Transfer Techniques , Genetic Vectors/genetics , Herpesvirus 1, Human/genetics , Transgenes/genetics , Genetic Engineering , Herpesvirus 1, Human/physiology
6.
J Indian Med Assoc ; 2008 Jan; 106(1): 42, 44
Article in English | IMSEAR | ID: sea-99922

ABSTRACT

Although exceedingly rare, fulminant hepatic failure in immunocompetent patients can develop with primary or recurrent infection due to herpes simplex virus. The diagnosis is frequently obscured by the absence of mucocutaneous involvement. Elevated transaminases with leucopenia and a relatively low bilirubin level may provide clues to the diagnosis. Here a female patient, 43 years, presented with the complaints of increasing jaundice, anorexia, nausea, vomiting for one week duration. She had hepatomegaly. Investigations revealed markedly raised transaminases and coagulopathy. Herpes simplex virus IGM (by ELISA) was positive. The immunocompetent woman was treated with acyclovir but the result was fatal.


Subject(s)
Adult , Antibodies, Viral/analysis , Biopsy , Diagnosis, Differential , Enzyme-Linked Immunosorbent Assay , Fatal Outcome , Female , Herpes Simplex/complications , Herpesvirus 1, Human/genetics , Humans , Liver/pathology , Liver Failure, Acute/diagnosis
7.
Indian J Ophthalmol ; 2007 Jul-Aug; 55(4): 308-10
Article in English | IMSEAR | ID: sea-69711

ABSTRACT

A case of bilateral acute retinal necrosis due to herpes simplex virus 1, in a child is reported. The case presented as an extensive hemorrhagic retinopathy that was misdiagnosed as non-infective initially. Diagnostic aqueous tap of the blind eye for viral DNA by polymerase chain reaction helped to confirm viral etiology when the other eye was affected. Appropriate antiviral therapy followed by prompt surgeries for subsequent retinal detachment helped to salvage useful vision in the second eye.


Subject(s)
Acute Disease , Adolescent , DNA, Viral/genetics , Eye Infections, Viral/complications , Follow-Up Studies , Herpes Simplex/complications , Herpesvirus 1, Human/genetics , Humans , Laser Coagulation , Male , Necrosis/complications , Retina/pathology , Retinal Hemorrhage/etiology , Severity of Illness Index , Vitrectomy
8.
Indian J Med Microbiol ; 2005 Oct; 23(4): 239-44
Article in English | IMSEAR | ID: sea-54111

ABSTRACT

PURPOSE: To standardize and apply a polymerase chain reaction (PCR) on the glycoprotein D gene to differentiate Herpes simplex virus (HSV) 1 & 2 serotypes in culture negative intraocular specimens. METHODS: Twenty-one intraocular fluids collected from 19 patients were subjected to cultures for HSV and uniplex PCR (uPCR) for DNA polymerase gene. To differentiate HSV serotypes, as 1 & 2, a seminested PCR (snPCR) targeting the glycoprotein D gene was standardised and applied onto 21 intraocular fluids. The specificity of the snPCR was verified by application onto ATCC strains of HSV 1 and 2, clinical isolates and DNA sequencing of the amplified products. All specimens were also tested for the presence of cytomegalovirus (CMV) and varicella zoster virus (VZV) by nucleic acid amplification methods. RESULTS: Four of the 21 intraocular fluids were positive for HSV by uPCR. snPCR detected HSV in three additional specimens (total of seven specimens), and identified three as HSV 1 and four as HSV 2. DNA sequencing of PCR products showed 100% homology with the standard strains of HSV 1 and 2 respectively. None of the samples were positive in culture. Among the other patients, CMV DNA was detected in two and VZV DNA in five others. CONCLUSIONS: The standardized snPCR can be applied directly onto the culture negative specimens for rapid differentiation of HSV serotypes.


Subject(s)
DNA, Viral/analysis , DNA-Directed DNA Polymerase/analysis , Diagnosis, Differential , Exodeoxyribonucleases/analysis , Herpesviridae Infections/diagnosis , Herpesvirus 1, Human/genetics , Herpesvirus 2, Human/genetics , Humans , Polymerase Chain Reaction/methods , Retinitis/diagnosis , Viral Envelope Proteins/analysis , Viral Proteins/analysis
9.
Article in English | IMSEAR | ID: sea-34186

ABSTRACT

Whole genomic polymorphisms for 20 HSV-1 and 20 HSV-2 isolates from Thai patients were analyzed by means of Restriction Fragment Length Polymorphism (RFLP) analysis using 4 restriction endonucleases: BamHI, Kpnl, HindIII, and EcoRI. Variations in cleavage sites among the HSV-1 and HSV-2 isolates were compared to the cleavage patterns of standard HSV-1 strain KOS and HSV-2 strain Baylor 186. Although 70% of HSV-1 isolates with BamHI digestion, 50% with Kpnl, 75% with HindIII and 70% with EcoRI digestion were found to be similar to the standard HSV-1 (KOS) pattern, new BamHI restriction sites were detected in some HSV-1 isolates. For HSV-2 isolates, 85% had the same pattern as the standard HSV-2 (Baylor 186) after digestion with BamHI, HindIII, and EcoRI. No difference was observed with Kpnl digestion. When the patterns from the 4 enzymes were combined, HSV-1 isolates showed more divergence than the HSV-2 isolates. HSV-1 isolates found in both non-genital and genital lesions had more variety than the HSV-2 isolates. This suggests that intratypic variations in HSV-2 are fewer than in HSV-1.


Subject(s)
DNA, Viral , Genetic Variation , Herpesvirus 1, Human/genetics , Herpesvirus 2, Human/genetics , Humans , Molecular Sequence Data , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Thailand
10.
Neurol India ; 2003 Sep; 51(3): 341-4
Article in English | IMSEAR | ID: sea-120354

ABSTRACT

BACKGROUND: Clinical criteria (symptoms) are not reliable enough to differentiate between different causes of encephalitis. The clinical presentation of herpes simplex virus encephalitis (HSVE) is not classically constant and in such a patient, therefore, it is vital to make early diagnosis. AIMS: To investigate satisfactory and crucial clinical signs as guide to perform HSV-PCR in a rapid diagnosis of herpes simplex virus encephalitis. MATERIAL AND METHODS: A total of 156 CSF specimens from 70 patients with clinically suspected HSVE or meningoencephalitis were tested. The criteria for cases suspected of HSVE were fever >380C, altered mental status and other critical manifestations. CSF features, irregularity in brain CT scan and MRI findings were also assessed. All the specimens were collected before and after Acyclovir treatment. Polymerase chain reaction was performed using primers, which amplified DNA sequences for both HSV-1 and HSV-2. STATISTICAL ANALYSIS: To analyze data, two-tailed Fisher's exact test and the X2-test with Yates' correction were used as appropriate. The odds ratio was used to express the strength of association between the clinical factors and the PCR results. RESULTS: HSV-DNA was detected in 18% of the specimens, belonging to 25.7% of the patients. Results indicate that the majority of the clinical symptoms are not specific to definitive clinical diagnosis of HSVE, except alteration in the level of consciousness--odds ratio [0.27 (0.07-0.96) (P=0.033)]; and lateralization sign--odds ratio [4.7 (0.98-22.6) (P=0.023)]. However, laboratory data, including total white blood cell count, especially the number of lymphocytes, and MRI findings could be suggested for HSV-PCR examination. CONCLUSION: At the first admission, a preliminary finding of at least two important clinical features mentioned above along with the pattern of CSF cell and differential counts could be sufficient to perform HSV-PCR which could ultimately result in a rapid and correct diagnosis of herpes simplex encephalitis.


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Cross-Sectional Studies , Female , Herpesvirus 1, Human/genetics , Herpesvirus 2, Human/genetics , Humans , Male , Middle Aged , Polymerase Chain Reaction , Severity of Illness Index
11.
Indian J Ophthalmol ; 2003 Mar; 51(1): 71-5
Article in English | IMSEAR | ID: sea-70099

ABSTRACT

PURPOSE: To evaluate the diagnostic usefulness of enzyme linked immuno-sorbent assay (ELISA) in single serum samples to associate herpes simplex virus (HSV), varicella zoster virus (VZV) or cytomegalovirus (CMV) with viral retinitis as against polymerase chain reaction (PCR) on intraocular specimens. It was also designed to study the seroprevalence in normal healthy individuals, and the genomic prevalence of HSV, VZV and CMV in patients without an active viral inflammatory process. METHODS: PCR for the detection of HSV, VZV and CMV genomes was done on 33 and 90 intraocular fluids from viral retinal patients and non-viral controls respectively. ELISA was done on 30 and 100 serum samples from viral retinitis patients and normal healthy controls respectively. RESULTS: PCR did not detect HSV, VZV and CMV genomes except one, in which VZV-DNA was detected. ELISA showed prevalence rates of 28%, 83% and 90% for antibodies against HSV, VZV and CMV respectively in the normal population. In the 30 viral retinitis patients, PCR detected HSV-DNA in 2 (6.7%), VZV-DNA in 7 (23.3%) and CMV-DNA in 6 (20.0%) patients, while ELISA detected antibodies against HSV, VZV and CMV in 13 (43.3%), 24 (80.0%) and 23 (76.7%) patients respectively. ELISA was of value in indirect diagnosis only in 6 (20.0%) as compared to 15 (50.0%) of 30 patients by PCR, this difference was statistically significant (McNemar test, P value = 0.005). CONCLUSION: Serology by ELISA is no longer a useful diagnostic tool to associate HSV, VZV and CMV viruses with viral retinitis.


Subject(s)
Adolescent , Adult , Antibodies, Viral/blood , Cytomegalovirus/genetics , Cytomegalovirus Retinitis/diagnosis , DNA, Viral/analysis , Enzyme-Linked Immunosorbent Assay , Eye Infections, Viral/diagnosis , Genome, Viral , Herpes Simplex/diagnosis , Herpes Zoster Ophthalmicus/diagnosis , Herpesvirus 1, Human/genetics , Herpesvirus 3, Human/genetics , Humans , Polymerase Chain Reaction , Retinitis/diagnosis , Sensitivity and Specificity
12.
Indian J Ophthalmol ; 2000 Jun; 48(2): 140-1
Article in English | IMSEAR | ID: sea-71067

ABSTRACT

The present report describes a case where HSV was detected by polymerase chain reaction (PCR) in the lens cortical material removed during cataract surgery one year after resolution of retinal inflammation in a patient with ARN.


Subject(s)
Adult , DNA Primers/chemistry , DNA, Viral/analysis , Diagnosis, Differential , Eye Infections, Viral/diagnosis , Herpes Simplex/diagnosis , Herpesvirus 1, Human/genetics , Humans , Lens Diseases/diagnosis , Male , Polymerase Chain Reaction , Retinal Necrosis Syndrome, Acute/diagnosis
13.
Medicina (B.Aires) ; 58(2): 185-8, 1998. ilus
Article in Spanish | LILACS | ID: lil-212792

ABSTRACT

Células tumorales transducidas con vectores retrovirales portanto el gen de la timidina kinasa del virus herpes simplex-1 (HSV-tk), son capaces de transformar la droga antiherpética ganciclovir (GCV) en un metabolito tóxico para células en división. Esta terapia suicida aumenta su eficiencia debido a un efecto "bystander" que induce la muerte de células no transducidas, vecinas a células modificadas. El mecanismo del mencionado efecto no se conoce totalmente, pero existe evidencia que asigna un rol preponderante al sistema inmune, para lograr una completa erradicación tumoral. En este trabajo estudiamos la efectividad del sistema en tres líneas celulares: un melanoma humano y uno murino, y un glioma de rata. Los tumores fueron estabelecidos por inyección de células tumorales s.c. en ratones nude y C57BI/6, e intracerebralmente por esterotaxis en ratas Sprague Dawley, respectivamente. Animales tratados fueron co-inyectados con células productoras de retrovirus expresando HSV-tk y posterior administración i.p. de GCV. En experimentos in vivo a corto plazo, se observó inhibición total o parcial del crecimiento tumoral en todos los modelos. En experimentos de supervivencia a largo plazo con células C6, el 50 por ciento de los animales sobrevivió más de 75 dias (p < 0 0001) y fue capaz de rechazar un desafio con células parentales C6 inyectadas en el hemisferio contralateral. El análisis histológico e inmunohistoquímico mostró la presencia de un infiltrado inflamatorio compuesto por linfocitos T, macrófagos y polimorfonucleares. Estos resultados demuestran que el uso de genes suicidas puede ser una herramienta de enorme importancia en el tratamiento de tumores de cerebro y de metástasis cerebrales.


Subject(s)
Animals , Mice , Rats , Antimetabolites/pharmacology , Brain Neoplasms/therapy , Ganciclovir/pharmacology , Gene Transfer Techniques , Genetic Therapy/methods , Glioma/therapy , Melanoma, Experimental/therapy , Thymidine Kinase/genetics , Brain/pathology , Cell Death/drug effects , Cell Division/drug effects , Genetic Vectors , Herpesvirus 1, Human/genetics
14.
Rev. méd. Chile ; 124(2): 153-9, feb. 1996. ilus, tab
Article in Spanish | LILACS | ID: lil-173316

ABSTRACT

Two herpes simplex viral serotypes, HSV type and HSV type 2 may cause genital herpes. The aim of this study was to perform a genetical analysis and characterization of virus isolated from 4 patients with double genital herpetic infections. In 11 viral isolated, the cytopathic effect in Vero cells was studied, the antigenic type was determined using monoclonal antibodies and genomic analysis was performed with Eco RI, Hind III and Bgl II enzymes. Five viral isolates generated a diffuse and 6 a localized cytopathic effect. Monoclonal antibodies identified four HSV-1 and seven HSV-2. Genomic analysis had concordant results. Four HSV-1 were obtained, with different genomic patterns within them; 3 were different to the standard North American strain. The seven HSV-2 obtained had 3 different types of electrophoretic profiles, thet were different to the standard North American strain. It is concluded that the genomic and antigenic analysis allowed in the detection of herpetic genital infections caused by herpes virus type I and 2 in the same individual and the identification of herpes virus strains with distinct regional characteristics


Subject(s)
Humans , Female , Antigens, Viral/genetics , Genome, Viral , Herpes Genitalis/genetics , Herpes Simplex/genetics , DNA, Viral/analysis , Herpesvirus 1, Human/genetics , Herpesvirus 2, Human/genetics , Serotyping/methods
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