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1.
Medisan ; 24(1)ene.-feb. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1091162

ABSTRACT

Introducción: Las enfermedades oftalmológicas originadas por virus ocupan un lugar importante en el quehacer diario de la especialidad. Objetivo: Caracterizar pacientes con infecciones oculares por herpesvirus según variables clínicas y epidemiológicas de interés. Métodos: Se realizó un estudio descriptivo y transversal de 29 pacientes adultos con infecciones por herpesvirus, atendidos en la consulta oftalmológica del Hospital General Docente Dr. Juan Bruno Zayas Alfonso de Santiago de Cuba, desde septiembre de 2012 hasta igual mes de 2013. Resultados: En la casuística predominaron los pacientes de piel blanca (55,1 %), el sexo femenino (69,0 %) y el grupo etario de 57 años y más (48,3 %). El ojo rojo y la inyección cilioconjuntival constituyeron los síntomas y signos más comunes (75,9 %). Las lesiones más frecuentes estuvieron localizadas en los párpados, la piel y la córnea (31,0 %). La afectación de la agudeza visual por debajo de 0,3 estuvo relacionada con la ocurrencia de afectación estromal y la localización de las lesiones en el área pupilar. Ningún paciente presentó inflamación del segmento posterior. Conclusiones: No se encontraron argumentos referidos a la relación existente entre la localización de las lesiones y la afectación de la agudeza visual, lo cual impidió realizar comparaciones.


Introduction: Eye diseases originated due to virus occupy an important place in daily routine of the specialty. Objective: To characterize patients with eye infections due to herpesvirus according to clinical and epidemiological variables of interest. Methods: A descriptive and cross-sectional study of 29 adult patients with infections due to herpesvirus was carried out, they were assisted in the ophthalmologic service of Dr. Juan Bruno Zayas Alfonso Teaching General Hospital in Santiago de Cuba, from September, 2012 to the same month in 2013. Results: In the case material white skin patients (55.1 %), female sex (69.0 %) and 57 years and over age group (48.3 %) prevailed. Red eye and ciliumconjuntival injection constituted the most common symptoms and signs (75.9 %). The most frequent lesions were located in the eyelids, the skin and cornea (31.0 %). The affectation of the visual acuteness below 0.3 was related to the occurrence of stromal affectation and the localization of lesions in the pupilar area. No patient presented inflammation of the later segment. Conclusions: Arguments about the existent relationship between the localization of lesions and the affectation of the visual acuteness were not found, which prevented to carry out comparisons.


Subject(s)
Eye Infections, Viral , Eye Infections, Viral/epidemiology , Herpesvirus 2, Human , Herpesvirus 1, Human , Eye Diseases
2.
Medicina (B.Aires) ; 79(6): 513-515, dic. 2019. tab
Article in Spanish | LILACS | ID: biblio-1056762

ABSTRACT

La meningitis linfocítica recurrente o meningitis de Mollaret es una entidad asociada a un gran número de etiologías infecciosas, autoinmunes, toxicológicas y neoplásicas. En la actualidad el virus herpes simple tipo 2 (HSV-2) es el agente más frecuentemente aislado. Afecta frecuentemente a mujeres de mediana edad y tiende a autolimitarse sin secuelas dentro de la primera semana de inicio de síntomas. El diagnóstico se basa en la detección de ácidos nucleicos virales en el líquido cefalorraquídeo. Al momento no se ha demostrado beneficio en el uso de tratamiento antiviral en la prevención de recurrencias.


Recurrent lymphocytic meningitis or Mollaret´s meningitis is a rare condition caused by a number of infectious, autoimmune, toxic and neoplastic diseases. Herpes simplex type 2 is the most commonly isolated agent. It usually compromises middle aged women, with a self-limited clinical presentation that resolves within a week leaving no sequelae. Its diagnosis is mainly based on nucleic acid detection on cerebrospinal fluid. Antiviral prophylaxis has not shown conclusive to avoid recurrences.


Subject(s)
Humans , Female , Middle Aged , Herpesvirus 2, Human/isolation & purification , Herpes Simplex/complications , Meningitis, Viral/virology , Antiviral Agents , Recurrence , Acyclovir/therapeutic use , Polymerase Chain Reaction , Meningitis, Viral/drug therapy
3.
Rev. bras. neurol ; 55(3): 9-12, jul.-set. 2019. ilus
Article in Portuguese | LILACS | ID: biblio-1022875

ABSTRACT

A meningite recorrente linfocítica benigna ou meningite de Mollaret, inicialmente descrita pelo neurologista francês Pierre Mollaret em 1944, é uma condição relativamente rara, benigna mas incapacitante durante os seus períodos de agudização. Trata-se de quadro inflamatório meníngeo recorrente devido a reativação de infecção pelo herpes simples vírus, particularmente o herpesvirus do tipo 2 (HSV-2). Pode ser reconhecida a partir do seu quadro clínico de meningismo agudo, perfil liquórico linfocítico e identificação do genoma viral por PCR no líquor. Aciclovir e seus derivados podem ser utilizado no seu tratamento ou na sua profilaxia. Sua identificação é importante no sentido de se excluir outras causas de quadros meníngeos recorrentes.


Benign recurrent lymphocytic meningitis or Mollaret's meningitis (MM) was frst described by the French neurologist Pierre Mollaret in 1944. MM is a relatively rare, benign but disabling condition. MM is a recurrent meningeal inflammatory illness due to reactivation of herpes simplex virus infection, particularly herpesvirus type 2 (HSV-2). It can be recognized from its clinical picture of acute meningism, lymphocytic CSF profle and by the identifcation of the viral genome in the CSF by PCR. Acyclovir and its derivatives may be used for its treatment or prophylaxis. The identifcation of MM is important in order to exclude other causes of recurrent meningeal conditions.


Subject(s)
Humans , Female , Adult , Middle Aged , Herpes Simplex/diagnosis , Herpes Simplex/etiology , Meningitis, Aseptic/diagnosis , Meningitis, Aseptic/drug therapy , Acyclovir/therapeutic use , Herpesvirus 2, Human/pathogenicity , Diagnosis, Differential , Neurology/history
4.
Neonatal Medicine ; : 63-66, 2019.
Article in English | WPRIM | ID: wpr-741662

ABSTRACT

Herpes simplex virus (HSV) is a common pathogen, that causes a broad spectrum of diseases, ranging from minor skin infections to severe encephalitis and widespread infections. Acute retinal necrosis (ARN), one of the most serious manifestations of HSV infection, is defined as a rapidly progressing necrotizing retinopathy that presents discrete areas of circumferential retinal necrosis, along with signs of uveitis, vitreitis, and retinal vasculitis. We encountered a case of a female infant, born at 33 weeks of gestation with a body weight at birth of 2,080 g, who had ARN and encephalomalacia due to HSV infection. ARN associated with HSV infection should be suspected when nonspecific retinal exudates are observed in neonates, especially preterm infants.


Subject(s)
Body Weight , Encephalitis , Encephalomalacia , Exudates and Transudates , Female , Herpes Simplex , Herpesvirus 2, Human , Humans , Infant , Infant, Newborn , Infant, Premature , Necrosis , Parturition , Pregnancy , Retinal Necrosis Syndrome, Acute , Retinal Vasculitis , Retinaldehyde , Simplexvirus , Skin , Uveitis
5.
Article in Chinese | WPRIM | ID: wpr-813038

ABSTRACT

To explore the antiviral activity of nano-realgar against herpes simplex virus Type II (HSV-2) in vitro.
 Methods: Acyclovir (ACV) as a positive control, the cytotoxicity of nano-realgar at different concentrations (including 200.00, 150.00, 100.00, 50.00, 25.00, 12.50, 6.25, 3.13, 1.54, 0.78, 0.39 and 0 mg/L) on normal Vero cells were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. HSV-2 virus titer was determined by plaque assay, and the Vero cells model of HSV-2 infection was established. Subsequently, the antiviral effects of nano-realgar at different concentrations (including 20.00, 10.00, 5.00, 2.50, 1.25, 0.63, 0.31, 0.15, 0.08, 0.04 and 0 mg/L) on infected cells model were evaluated by the observation of cytopathic effect (CPE) and MTT method under the 3 modes including pre-treatment, treatment and direct inactivation.
 Results: The 50% cytotoxic concentration (CC50) of nano-realgar on Vero cells was 37.15 mg/L. The titer of HSV-2 was 7.30 log PFUs/mL. In the 3 modes, the half-maximal effective concentration (EC50) of nano-realgar on HSV-2 infected Vero cells were 0.13, 1.80 and 0.52 mg/L, and the corresponding therapeutic index (TI) were 285.77, 20.64, 71.44, respectively. The TI value of nano-realgar on pre-treatment mode was higher than that of nano-realgar on treatment and direct inactivation modes.
 Conclusion: Nano-realgar can play a good anti-HSV-2 activity in the 3 modes (pre-treatment, treatment and direct inactivation), and the anti-HSV-2 efficacy of nano-realgar on pre-treatment mode is better than that of nano-realagr on other 2 modes.


Subject(s)
Animals , Antiviral Agents , Arsenicals , Chlorocebus aethiops , Herpesvirus 1, Human , Herpesvirus 2, Human , Sulfides , Vero Cells
7.
Article in English | WPRIM | ID: wpr-717056

ABSTRACT

BACKGROUND: Human herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) are responsible for a plethora of human diseases, of which cutaneous and mucocutaneous infections are the most prevalent. In its most severe form, HSV infection can cause meningitis/encephalitis. We compared the Luminex ARIES HSV 1&2 assay (Luminex Corp., Austin, TX, USA), an automated sample-to-result molecular solution, to two non-automated HSV DNA assays. METHODS: A total of 116 artificial controls were used to determine the analytical performance of the ARIES assay. Controls were prepared by spiking universal transport medium (UTM) and cerebrospinal fluid (CSF) samples from patients who tested negative for HSV by an in-house HSV-1 and -2 DNA assay with reference materials (SeraCare Life Sciences, MA, USA; ZeptoMetrix Corp., MA, USA). Another 117 clinical samples were then used to compare the clinical performance of the ARIES assay with those of an in-house assay and the FTD Neuro 9 assay (Fast Track Diagnostics, Junglinster, Luxembourg). RESULTS: The analytical sensitivity (95% limit of detection) of the ARIES assay was 318 copies/mL (UTM samples) and 935 copies/mL (CSF samples) for HSV-1 strain 96 and 253 copies/mL (UTM samples) and 821 copies/mL (CSF samples) for HSV-2 strain 09. No cross-reactivity was observed in samples spiked with 14 non-HSV microorganisms. Compared with the reference result (agreement between the in-house and FTD Neuro 9 results), the ARIES assay had overall concordance rates of 98.2% (111/113) and 100% (113/113) for HSV-1 and HSV-2, respectively. CONCLUSIONS: The ARIES assay appears to be an excellent alternative for rapid detection and differentiation of HSV in skin and genital infections, meningitis, and encephalitis.


Subject(s)
Biological Science Disciplines , Cerebrospinal Fluid , DNA , Encephalitis , Herpes Simplex , Herpesvirus 1, Human , Herpesvirus 2, Human , Humans , Meningitis , Real-Time Polymerase Chain Reaction , Simplexvirus , Skin
8.
Article in English | WPRIM | ID: wpr-740100

ABSTRACT

BACKGROUND: Human papillomavirus (HPV) genotypes that infect the genital tract play a main etiologic role in cervical cancer progression. Other environmental factors, such as sexually transmitted diseases and the host genetic pattern, contribute to infection persistence of the uterus and cervical epithelium in sustaining their malignancy. The Janus kinase 2 is a non-receptor tyrosine kinase in cell signaling process of tumor genesis. In the present study, JAK2 V167F mutation was distinguished in women with sexually transmitted infections, such as Herpes simplex virus 2, Chlamydia trachomatis and Mycoplasma genitalium and cervical cancer. METHODS: This case-control survey was performed on 195 liquid based cytology of women specimens. Fifty, 98, and 47 samples were from women with known cervical cancer, HPV positive and HPV negative, respectively. Single nucleotide polymorphism analysis, sexually transmitted infections detection and HPV genotyping were carried out using approved PCR- RFLP, in-house multiplex TaqMan Real Time PCR and the reverse dot blot hybridization assay. RESULTS: HPVs 6, 16, 18, 11, 31, and 51 were the most common genotypes. The prevalence rate of multiple HPV genotypes was 46.0% to 10.1%. Analysis of JAK2 V617F (1849 G > T) showed that prevalence of mutation was GG (65.1%), GA (34.9%), and TT (0%), respectively. There were no statistically significant differences between this mutation and variables of population survey (P ≥ 0.05). CONCLUSIONS: The molecular epidemiology study on the genetic polymorphisms, i.e., JAK2 V617F and other single nucleotide polymorphisms as a diagnostic tool is necessary for cancer screening and prophylactic programs.


Subject(s)
Case-Control Studies , Chlamydia trachomatis , Early Detection of Cancer , Epithelium , Female , Genotype , Herpesvirus 2, Human , Humans , Iran , Janus Kinase 2 , Molecular Epidemiology , Mycoplasma genitalium , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Polymorphism, Single Nucleotide , Prevalence , Protein-Tyrosine Kinases , Real-Time Polymerase Chain Reaction , Sexually Transmitted Diseases , Uterine Cervical Neoplasms , Uterus
9.
Mem. Inst. Oswaldo Cruz ; 113(12): e180328, 2018. tab, graf
Article in English | LILACS | ID: biblio-1040588

ABSTRACT

BACKGROUND Human herpesvirus 2 (HHV-2) have DNA genome with a limited genetic variability and have been classified into two clades. OBJECTIVES To identify and characterise six HHV-2 isolates derived from Brazilian women. METHODS HHV-2 isolates were performed polymerase chain reaction (PCR) and sequencing of 2250 pb of the glycoprotein B (gB) coding regions. FINDINGS Four HHV-2 isolates were classified into clade B, while the remaining two, derived from HIV-1 co-infected women, showed a notable genetic divergence (> 1%). MAIN CONCLUSION The results reveal novel HHV-2 variants. The impact of these novel variants on HHV-2 pathogenesis and HIV/HHV-2 coinfection need to be investigated.


Subject(s)
Humans , Female , Herpes Genitalis/virology , HIV Infections/virology , HIV-1 , Herpesvirus 2, Human/genetics , Genes, Viral/genetics , Phylogeny , Herpes Genitalis/complications , HIV Infections/complications , Polymerase Chain Reaction , Bertholletia , Coinfection/virology
10.
LMJ-Lebanese Medical Journal. 2018; 66 (1): 57-60
in English | IMEMR | ID: emr-170977

ABSTRACT

Acute transverse myelitis, an acute inflammation of the spinal cord, can be caused by HSV infection. We report a case of an 85-year-old man who presented with lower extremity weakness and flare-up of a vesicular rash on the sacral area. The patient was diagnosed with acute transverse myelitis based on MRI and CSF findings. CSF analysis showed a lymphocytic pleocytosis and positive HSV-2 PCR. The patient was started on intravenous acyclovir, with full clinical and radiologic improvement despite persistence of HSV-2 PCR positivity in the CSF for four months. A review of the literature of HSV myelitis is included. We conclude that testing of HSV PCR on repeated CSF samples might not accurately reflect viral activity in the setting of clinical improvement


Subject(s)
Humans , Male , Aged, 80 and over , Herpesvirus 2, Human , Virus Replication , Polymerase Chain Reaction
11.
IBJ-Iranian Biomedical Journal. 2018; 22 (2): 123-128
in English | IMEMR | ID: emr-192459

ABSTRACT

Background: Herpes simplex virus type 2 [HSV-2] is a common infection in human immunodeficiency virus [HIV] patients and may accelerate HIV progression by rising HIV viral load and decreasing CD4 count. However, the available data regarding the influence of HSV-2 seropositivity on HIV progression in HIV individuals are inconclusive. Therefore, we aimed to determine HSV-2 seroprevalence in naive HIV patients and normal controls and also investigate the relation of HIV viral load and CD4 count with HSV-2 seropositivity. Subsequently, we investigated the association of HSV-2 serostatus with changing in CD4 count and HIV viral load in our subjects, after one year follow-up


Methods: In this study, 116 naive HIV patients and 85 healthy controls from Tehran, Iran were enrolled. HSV-2 IgG antibody was detected by ELISA. CD4 count was determined by flowcytometry, and serum HIV RNA copy numbers were determined using real-time PCR


Results: The prevalence of HSV-2 IgG was 18.1% in naive HIV patients and 0% in the control group [P = 0.000]. HSV-2 seroconversion was observed in 2.43% of HIV patients after one year. There was no significant difference regarding HSV-2 serostatus with CD4 count and HIV RNA viral load in our study cohort at baseline and after one year


Conclusion: Our results revealed that the prevalence and incidence of HSV-2 infection are low in our HIV cases, and it is negligible in the control group. However, it seems that HIV/HSV2 co-infection has no role on HIV infection acceleration


Subject(s)
Humans , Male , Female , Adult , HIV Infections , Herpesvirus 2, Human , Herpes Genitalis , Disease Progression , Viral Load , CD4 Lymphocyte Count
12.
Arq. bras. oftalmol ; 80(2): 84-87, Mar.-Apr. 2017. tab
Article in English | LILACS | ID: biblio-838786

ABSTRACT

ABSTRACT Objective: Bacterial keratitis occurs worldwide, and despite recent developments, it remains a potentially blinding condition. This study assesses the presence of herpes simplex virus (HSV-1 and -2) and varicella zoster virus (VZV) by quantitative real-time polymerase chain reaction (qPCR) in corneal scrapings from patients with bacterial keratitis. Methods: A total of 65 patients with clinical diagnoses of infectious corneal ulcers prospectively underwent clinical eye examinations. Corneal scrapings were investigated by Gram staining, Giemsa staining, culture, and qPCR (the study group). Risk factors and epidemiological data were recorded. The control group comprising 25 eyes with typical herpes dendritic keratitis was also analyzed by qPCR. Results: From the study group (n=65), nine patients (13.8%) had negative smears, cultures, and qPCR findings. Fifty-six (86.2%) patients had positive cultures: 51 for bacteria, 4 for fungi, and 1 for amoebae. Of the patients who had positive bacterial cultures, qPCR identified 10 patients who were also positive for virus: one for VZV and nine for HSV-1. Of the 25 patients in the control group, 21 tested positive for HSV-1 by qPCR analysis. Conclusions: Herpes may be present in patients with bacterial corneal ulcers, and qPCR may be useful in its detection.


RESUMO Objetivo: Ceratites bacterianas ocorrem mundialmente e apesar dos novos desenvolvimentos permanece como uma condição que pode levar à cegueira. Avaliar a presença de herpes simples (-1 e -2) e vírus varicella zoster (VZV) por reação em cadeia quantitativa de polimerase em tempo real (qPCR) em raspados corneanos de pacientes com ceratite bacteriana. Métodos: Sessenta e cinco pacientes com ceratite infecciosa foram submetidos a raspados corneanos estudados para gram, Giemsa, cultura e qPCR (grupo de estudo). Foram avaliados fatores de risco e epidemiológicos. O grupo controle foi composto por 25 casos de úlcera dendrítica típica por herpes analisados por qPCR. Resultados: Do grupo de estudo (n=65), nove pacientes (13,8%) apresentaram cultura, qPCR e raspado negativos. Cinquenta e seis (86,2%) pacientes apresentaram cultura positiva, 51 para bacteria, 4 para fungo e 1 para ameba. A qPCR identificou 10 pacientes do grupo de cultura positiva para bactéria que também foram positivos para vírus, um VZV e 9 para HSV-1. Dos 25 pacientes que compunham o grupo controle, 21 apresentaram qPCR positivo para HSV-1. Conclusão: Herpes pode estar presente em pacientes com úlceras de córnea bacterianas e a qPCR pode ser útil na sua detecção.


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Keratitis, Dendritic/microbiology , Herpesvirus 2, Human/isolation & purification , Herpesvirus 1, Human/isolation & purification , Herpesvirus 3, Human/isolation & purification , Cornea/virology , Real-Time Polymerase Chain Reaction/methods , Keratitis/microbiology , DNA Probes , Eye Infections, Bacterial/microbiology , Keratitis, Dendritic/diagnosis , Keratitis, Dendritic/virology , Prospective Studies , Keratitis/diagnosis , Keratitis/virology
13.
Mem. Inst. Oswaldo Cruz ; 112(3): 220-223, Mar. 2017. tab, graf
Article in English | LILACS | ID: biblio-841773

ABSTRACT

The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.


Subject(s)
Humans , Herpesvirus 2, Human/genetics , Herpesvirus 1, Human/genetics , Herpes Simplex/virology , DNA, Viral/genetics , Sensitivity and Specificity , Viral Load , Real-Time Polymerase Chain Reaction , Herpes Simplex/diagnosis
14.
An. bras. dermatol ; 91(2): 216-218, Mar.-Apr. 2016. graf
Article in English | LILACS | ID: lil-781357

ABSTRACT

Abstract Neonatal herpes is a serious condition. Newborns can be contaminated in utero via transplacental hematogenic transmission, upon delivery (the most frequent route), or during the postnatal period (indirect transmission). Optimal management requires prompt and accurate recognition, particularly in newborns, in order to prevent complications. Acyclovir is the treatment of choice, but its implementation is often delayed while awaiting test results, such as PCR and serology. Cytology for diagnostic purposes is rarely used in dermatology, despite the quick and reliable results. We report a case of neonatal herpes caused by type 2 herpes simplex virus diagnosed by cytology.


Subject(s)
Humans , Male , Infant, Newborn , Pregnancy Complications, Infectious/pathology , Herpesvirus 2, Human , Herpes Simplex/pathology , Antiviral Agents/therapeutic use , Pregnancy Complications, Infectious/drug therapy , Skin/pathology , Acyclovir/therapeutic use , Cytological Techniques , Herpes Simplex/drug therapy
15.
Article in Korean | WPRIM | ID: wpr-179066

ABSTRACT

We describe two patients with acute myeloradiculitis associated with herpes simplex virus type 2 (HSV-2). They were previously healthy and immunocompetent and had no history of herpes infection or rash. Myeloradiculitis manifested as an acute flaccid paralysis that primarily involved the conus medullaris and cauda equina. laccid paralysis can be caused by HSV-2 myeloradiculitis, and so early antiviral treatment should be considered.


Subject(s)
Cauda Equina , Exanthema , Herpes Simplex , Herpesvirus 2, Human , Humans , Myelitis , Paralysis , Radiculopathy , Simplexvirus , Spinal Cord
16.
Article in Korean | WPRIM | ID: wpr-197552

ABSTRACT

BACKGROUND: Herpes simplex virus type 2 (HSV-2) is the second most common cause of viral meningitis and the most common cause of recurrent meningitis. Although the incidence of HSV-2 meningitis is high, its clinical characteristics are not well known. The purpose of this study was to review the clinical characteristics and prognosis of HSV-2 meningitis. METHODS: We analyzed patients who were admitted to the Department of Neurology at Severance Hospital with a final diagnosis of HSV-2 meningitis, as confirmed by applying the polymerase chain reaction to the cerebrospinal fluid (CSF) of patients. RESULTS: The study involved 998 patients with aseptic meningitis and 60 patients diagnosed with HSV-2 meningitis. The mean age at meningitis presentation was 32.5 years (range 18-54 years), and 72% of the patients were female. Common clinical symptoms were headache (100%), nausea and/or vomiting (83%), meningismus (57%), and fever (55%). Six patients had a history of genital herpes infection, and 11 had a past history of recurrent meningitis. The CSF study was notable for elevated protein (111.0±53.5 mg/dL, mean±standard deviation) and white cell count (332.0±211.3 cells/µL). The CSF/serum glucose ratio was 0.52±0.90. Various treatments were applied, including conservative care, antiviral agents, empirical antibiotics, and combined treatments. All patients recovered without serious neurologic sequelae. CONCLUSIONS: HSV-2 meningitis is relatively common, as are recurrent episodes. The clinical characteristics of HSV-2 meningitis are similar to those of other types of aseptic meningitis. HSV-2 meningitis is treated using antiviral therapy, and the prognosis is favorable even with conservative treatment.


Subject(s)
Anti-Bacterial Agents , Antiviral Agents , Cell Count , Cerebrospinal Fluid , Diagnosis , Female , Fever , Glucose , Headache , Herpes Genitalis , Herpes Simplex , Herpesvirus 2, Human , Humans , Incidence , Meningism , Meningitis , Meningitis, Aseptic , Meningitis, Viral , Nausea , Neurology , Polymerase Chain Reaction , Prognosis , Retrospective Studies , Simplexvirus , Vomiting
17.
Singapore medical journal ; : 346-352, 2015.
Article in English | WPRIM | ID: wpr-244781

ABSTRACT

<p><b>INTRODUCTION</b>Herpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit.</p><p><b>METHODS</b>We successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG(321-580His)) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG(321-580His) as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference.</p><p><b>RESULTS</b>In testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG(321-580His)-ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%.</p><p><b>CONCLUSION</b>The study indicates that gG(321-580His) has a high diagnostic potential for HSV-2 virus serodiagnosis in humans.</p>


Subject(s)
Adult , Antibodies, Viral , Blood , Enzyme-Linked Immunosorbent Assay , Female , Herpes Genitalis , Diagnosis , Virology , Herpes Simplex , Diagnosis , Virology , Herpesvirus 1, Human , Herpesvirus 2, Human , Humans , Immunoglobulin G , Chemistry , Male , Polymerase Chain Reaction , Reagent Kits, Diagnostic , Reference Values , Reproducibility of Results , Sensitivity and Specificity , Serologic Tests , Methods
18.
Article in Korean | WPRIM | ID: wpr-83634

ABSTRACT

Herpes simplex encephalitis is the most common form of encephalitis, but herpes simplex virus type 2 (HSV-2) encephalitis is very rare during pregnancy. In immunocompetent patients, HSV-2 encephalitis is usually mild. With early diagnosis and prompt initiation of antiviral treatment, as well as enhanced neurocritical care, a favorable outcome can be expected in both mother and neonate. We report a rare case of maternal HSV-2 encephalitis diagnosed by polymerase chain reaction following appendectomy. The woman had no symptomatic genital lesion, and the infant was not infected.


Subject(s)
Appendectomy , Early Diagnosis , Encephalitis , Encephalitis, Herpes Simplex , Female , Herpesvirus 2, Human , Humans , Infant , Infant, Newborn , Mothers , Polymerase Chain Reaction , Pregnancy , Simplexvirus , Skin
19.
Rev. argent. microbiol ; 46(3): 256-268, oct. 2014.
Article in Spanish | LILACS | ID: biblio-1015103

ABSTRACT

Los microbicidas constituyen una nueva herramienta, todavía en proceso de investigación, que podrían ayudar en la prevención de la infección por los virus de la inmunodeficiencia humana (Human immunodeficiency virus: HIV) y de otras infecciones de transmisión sexual (ITS). Una serie de evidencias ha demostrado que la complejidad de la transmisión sexual de patógenos virales requiere de la identificación de compuestos capaces de bloquear los eventos tempranos del ciclo de infección viral. En este manuscrito hacemos una revisión exhaustiva de las diferentes estrategias que se han estudiado o se están considerando para prevenir ITS mediante el uso de microbicidas, haciendo particular énfasis en aquellos con el potencial de bloquear la infección por el HIV. También se revisa el proceso complejo de evaluación preclínica que se requiere para llegar a estudios en humanos y se concluye con un breve análisis de las estrategias que podrían formar parte del futuro inmediato en la investigación de microbicidas


Microbicides are a new tool, still under investigation, which could help prevent infection by the human immunodeficiency virus (HIV) and other sexually transmitted infections (STIs). Increasing evidence shows that the complexity of sexual transmission of viral pathogens requires the identification of compounds able to block the early events during the cycle of viral infection. In this manuscript we provide a comprehensive review of the different microbicide strategies that have been studied or are currently being considered for STI prevention, particularly emphasizing those having the potential to block HIV infection. The manuscript also reviews the complex process that is required to conduct future clinical studies in humans and concludes with a brief discussion of the strategies that could be part of the immediate future in microbicide research


Subject(s)
Sexually Transmitted Diseases/prevention & control , Anti-HIV Agents/analysis , Papillomavirus Vaccines/analysis , Antiviral Agents/therapeutic use , Sexually Transmitted Diseases/diagnosis , Sexually Transmitted Diseases/therapy , Herpesvirus 2, Human/drug effects , Anti-Infective Agents/therapeutic use
20.
Article in Chinese | WPRIM | ID: wpr-302571

ABSTRACT

<p><b>OBJECTIVE</b>To explore the prevalence of syphilis and HSV-2 among female sex workers (FSWs) who use new types of drugs in Jiaozhou city.</p><p><b>METHODS</b>Through convenient sampling, an anonymous questionnaire survey was conducted among female sex workers to collect demographic characteristics, new-type drugs abusing characteristics and related sexual behaviors from October to December, 2013. Blood specimens were drawn for serological tests of syphilis antibody and HSV-2 antibody. Urine specimens of the subjects surveyed were collected to test for methamphetamine. Differences in demographic characteristics, new-type drug abusing characteristics, and sexual behaviours between drug-abusing FSWs and non-drug-abusing FSWs were compared by t-test and χ(2)-test.</p><p><b>RESULTS</b>A total of 460 FSWs were recruited in this study, and 105 FSWs admitted their drug abuse history. Among the 341 urine specimens confirmed to be methamphetamine positive, there were 3 FSWs claimed that they never abuse new-type drugs. The rate of new-type drug abuse was 23.5% (108/460). A total of 71.4% (75/105) of the new drug-abusing FSWs started using drugs under 25 years old. The main reasons for drug abuse were clients request (24, 22.9%), making more money (23, 21.9%) and companion temptations (22, 21.0%). Totally, 41.9% of them (44/105) took drugs with 4-5 persons, 32.4% (34/105) had sex with 2-3 men after taking drugs, and 60.2% of new-type drug-abusing FSWs (65/108) used condoms in the latest commercial intercourse, while only 7.4% FSWs (8/108) used condoms every time during their commercial sex activities in the recent month. Compared with FSWs having no drug abuse behavior, drug-abusing FSWs had higher single proportion (73.2% (79/108) vs 63.6% (224/352), χ(2) = 8.64, P < 0.05), lower condom use rate in the recent month (7.4% (8/108) vs 22.7% (80/352), χ(2) = 12.53, P < 0.01) and higher pregnancy rate in the recent 6 months (24.1% (26/108) vs 8.8% (31/352), χ(2) = 17.74, P < 0.01) and most of them come from the middle and high-level entertainmens (78.7% (85/108) vs 65.1% (229/352), χ(2) = 13.09, P < 0.01). Among the new-type drug-abusing FSWs, the prevalence rates of syphilis and HSV-2 were 12.0% (13/108) and 55.6% (60/108) , respectively. A total of 33 FSWs claimed that they were diagnosed with STDs in the recent year (30.6%) . The rates of syphilis(12.0% (13/108) vs 4.0% (14/352), χ(2) = 9.72, P < 0.01), HSV-2(55.6% (60/108) vs 39.2% (138/352), χ(2) = 9.01, P < 0.01) and diagnosed STDs (30.6% (33/108) vs 17.9% (63/352), χ(2) = 8.02, P < 0.01) among the drug-abusing FSWs were significantly higher than those of non-drug-abusing FSWs.</p><p><b>CONCLUSION</b>There is a higher proportion of new-type drug abuse among the FSWs in Jiaozhou, with significantly higher prevalence rates of syphilis and HSV-2 infection, compared with non-new types of drug abusing FSWs. Prevalent risk sexual behaviors and ignorance of new-types drugs' harm were seen among them.</p>


Subject(s)
China , Epidemiology , Condoms , Designer Drugs , Female , Herpes Genitalis , Epidemiology , Herpesvirus 2, Human , Humans , Methamphetamine , Pregnancy , Prevalence , Risk Factors , Risk-Taking , Sex Work , Sex Workers , Sexual Behavior , Sexual Partners , Substance-Related Disorders , Syphilis , Epidemiology
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