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1.
Rev. Col. Bras. Cir ; 46(1): e2068, 2019. tab, graf
Article in Portuguese | LILACS | ID: biblio-990362

ABSTRACT

RESUMO Objetivo: comparar o polimorfismo dos genes Glutationa S-transferase teta 1 (GSTT1) e Glutationa S-transferase mu 1 (GSTM1) da área do tumor com as margens proximal e distal de espécimes de estômago ressecados de pacientes com câncer gástrico, e investigar a presença do DNA do vírus Epstein-Barr (EBV) e Helicobacter pylori. Métodos: coletamos prospectivamente amostras teciduais da área do tumor e das margens de ressecção proximal e distal dos estômagos de dez pacientes com adenocarcinoma gástrico submetidos à gastrectomia com linfadenectomia D2 e submetemos esses espécimes à extração de DNA. Comparamos a área do tumor com as margens proximal e distal dos estômagos ressecados para o polimorfismo dos genes GSTT1 e GSTM1 e investigamos a presença de DNA do EBV e H. pylori. Utilizamos o exon 5 do gene p53 como controle interno da reação de PCR multiplex. Resultados: em um paciente, detectamos genótipos GSTT1 e GSTM1 nulos na área do tumor, em contraste com a presença de ambos os genes nas margens proximal e distal. Encontramos DNA do EBV e H. pylori na área do tumor e também nas margens proximal e distal. Em outro paciente, a margem proximal foi negativa para GSTT1 e o DNA do EBV foi negativo na margem distal. Em três pacientes, o EBV-DNA foi negativo apenas na margem distal. Conclusão: este é o primeiro relato em que diferentes genótipos, infecção por EBV-DNA e H. pylori foram observados no mesmo paciente, indicando provável deleção desses genes em resposta à progressão tumoral e heterogeneidade intratumoral.


ABSTRACT Objective: to compare the polymorphism of the Glutathione S-transferase theta 1 (GSTT1) and Glutathione S-transferase mu 1 (GSTM1) genes from the tumor area with the proximal and distal margins of stomach specimens resected from patients with gastric cancer, and to investigate the presence of Epstein-Barr virus (EBV) DNA and Helicobacter pylori. Methods: we prospectively collected tissue specimens from the tumor area and from the proximal and distal resection margins of the stomachs of ten patients with gastric adenocarcinoma who underwent gastrectomy with D2 lymphadenectomy, and submitted these specimens to DNA extraction. We compared the tumor area with the proximal and distal margins of the resected stomachs for polymorphism of GSTT1 and GSTM1 genes and investigated the presence of EBV-DNA and H. pylori. We used the p53 exon 5 gene as an internal control of the multiplex PCR reaction. Results: in one patient, we detected null GSTT1 and GSTM1 genotypes in the tumor area, in contrast to the presence of both genes in the proximal and distal margins. We found EBV-DNA and H. pylori in the tumor area and also in the proximal and distal margins. In another patient, the proximal margin was negative for GSTT1, and EBV-DNA was negative in the distal margin. In three patients, EBV-DNA was negative only in the distal margin. Conclusion: this is the first report where different genotypes, EBV-DNA and H. pylori infection were observed in the same patient, indicating a probable deletion of these genes in response to tumor progression and intratumoral heterogeneity.


Subject(s)
Humans , Male , Female , Aged , Polymorphism, Genetic/genetics , Stomach Neoplasms/surgery , Adenocarcinoma/surgery , Helicobacter pylori/genetics , Herpesvirus 4, Human/genetics , Stomach Neoplasms/enzymology , Stomach Neoplasms/microbiology , Stomach Neoplasms/virology , Adenocarcinoma/enzymology , Adenocarcinoma/microbiology , Adenocarcinoma/virology , Polymerase Chain Reaction , Prospective Studies , Risk Factors , Helicobacter pylori/isolation & purification , Herpesvirus 4, Human/isolation & purification , Genotype , Glutathione Transferase/genetics , Middle Aged
2.
Article in English | IMSEAR | ID: sea-140327

ABSTRACT

Background & objectives: A major drawback for genetic studies as well as long-term genotype-phenotype correlation studies in cancer is lack of representative human cell lines providing a continuous source of basic biomolecules and a system to carry out various experimental investigations. This can be overcome to some extent by establishing lymphoblastoid cell lines (LCLs) by infecting peripheral blood lymphocytes with Epstein Barr virus (EBV) which is known to immortalize human resting B cells in vitro giving rise to actively proliferating B-lymphoblastoid cell lines. The present study involves preparation and characterization of LCLs generated from patients with multiple primary neoplasms (MPN) of upper aero-digestive tract (UADT). Methods: Thirty seven LCLs were established from UADT MPN patients and healthy age, sex and habit matched controls using EBV crude stock. Characterization was done with respect to expression of CD-19 (Pan B-cell marker), CD3 (T cell specific marker), CD56 (NK-cell specific marker), cell morphology, ploidy analysis, genotype and gene expression comparison with the parent lymphocytes. Results: LCLs showed rosette morphology with doubling time of approximately 24 h. Ploidy analysis showed diploid DNA content which was maintained for at least 30 population doublings. When compared with parent lymphocytes there appeared no change at genetic and gene expression level. Interpretation & conclusions: Our results show that lymphoblastoid cell lines are a good surrogate of isolated lymphocytes bearing their close resemblance at genetic and phenotypic level to parent lymphocytes and are a valuable resource for understanding genotype-phenotype interactions.


Subject(s)
Herpesvirus 4, Human/analysis , Herpesvirus 4, Human/isolation & purification , Humans , Cell Line, Transformed , Cell Line , Neoplasms, Multiple Primary , Patients , Ploidies
3.
Article in English | WPRIM | ID: wpr-106769

ABSTRACT

BACKGROUND: Aggressive natural killer-cell leukemia (ANKL) is a rare neoplasm characterized by systemic proliferation of NK cells. However, the differential diagnosis of NK lymphoproliferative disorders is difficult because of the absence of a distinct diagnostic hallmark. Therefore, to identify diagnostic markers for ANKL, we analyzed the clinical data and laboratory findings obtained for 20 patients with ANKL. METHODS: From January 2000 to July 2007, 20 patients were diagnosed with ANKL on the basis of bone marrow studies. We retrospectively analyzed the clinical features and laboratory findings, including the complete blood count, Epstein-Barr virus status, immunophenotype, and the cytogenetic results. RESULTS: The subjects included 6 women and 14 men (median age, 44 yr; range, 2-70 yr). Cytogenetic studies were performed in 18 patients, and karyotypic abnormalities were observed in 9 patients (50%). None of the cytogenetic abnormalities were constantly observed in all the patients. However, 6q abnormalities were observed in 4 patients (4/18, 22%). The immunophenotype of the leukemic NK-cells was cytoplasmic CD3+, surface CD3-, CD16/56+, CD2+, and CD5-. Notably, the CD7 antigen was absent in 10 patients (50%). When the CD7 loss was combined with cytogenetic abnormalities, clonal markers could be identified in 75% of the ANKL cases. CONCLUSIONS: The CD7 antigen loss was frequently observed in our series of ANKL patients. In conjunction with the cytogenetic findings, this characteristic immunophenotypic finding can serve as a reliable marker for the timely diagnosis of ANKL. Therefore, immunophenotypic analysis of CD7 expression should be included in the diagnosis of NK cell neoplasms.


Subject(s)
Adolescent , Adult , Aged , Antigens, CD7/analysis , Blood Cell Count , Child , Child, Preschool , Cytogenetics , Female , Herpesvirus 4, Human/isolation & purification , Humans , Immunophenotyping , Karyotyping , Leukemia, Large Granular Lymphocytic/diagnosis , Male , Middle Aged , Retrospective Studies , Biomarkers, Tumor/analysis
4.
Article in English | WPRIM | ID: wpr-114574

ABSTRACT

BACKGROUND/AIMS: Epstein-Barr virus (EBV) is involved in the pathogenesis of angioimmunoblastic T-cell lymphoma (AILT), but its precise role and prognostic impact are not clear. This study aimed to evaluate the incidence of EBV-postitivity in the tumor and bone marrow (BM) samples from AILT patients, and their correlations with the clinical variables and patient survival. METHODS: Seventy AILT cases were identified over a period of 8 years. Twenty seven cases were investigated for their EBV tumor status, and 10 BM samples of these patients were investigated for their EBV status with using in situ hybridization (ISH). EBV PCR was performed for the BM mononuclear cells in 8 cases. RESULTS: Among the 27 tumor specimens, ten (37%) were EBV-positive. Only CD20-negativity in tumor correlated with the EBV-positivity (p=0.035). In 13 (48%) patients, gross tumor involvement was recognized by hematoxylin-eosin staining at the time of diagnosis. Among the 10 patients who had additional BM slides available, there were 3 with BM involvement, and none showed EBV positive results on ISH. EBV PCR of the BM mononuclear cells revealed one-positive case among 8 patients. This patient was negative for both BM involvement and EBV ISH. The median overall survival of the 25 treated patients was 48.9 months (95% CI: 18.6~79.2 months). Neither overall survival nor progression-free survival was related with EBV-positivity of the tumor. CONCLUSIONS: EBV-positivity of tumor had no impact on the prognosis of AILT patients.


Subject(s)
Adolescent , Adult , Aged , Bone Marrow/virology , DNA, Viral/isolation & purification , Female , Herpesvirus 4, Human/isolation & purification , Humans , Immunoblastic Lymphadenopathy/mortality , In Situ Hybridization , Lymphoma, T-Cell/mortality , Male , Middle Aged , Polymerase Chain Reaction , Prognosis , Survival Analysis
5.
Article in Korean | WPRIM | ID: wpr-34943

ABSTRACT

Lymphoepithelioma-like carcinoma (LELC) is an undifferentiated carcinoma with predominant lymphocytic infiltration, which is associated with Epstein-Barr virus (EBV) in variable proportions. We report two cases of carcinoma with predominant lymphoid stroma in hepatobiliary system. The first case was a lymphoepithelioma-like undifferentiated carcinoma with focal differentiation of cholangiocarcinoma (cytokeratin 19+) and hepatocellular carcinoma in light microscopy. The infiltration of CD8+ T lymphocytes was observed in the tumor and the surrounding hepatic parenchyme. In this tumor, EBV was detected and LMP1 was positive immunohistochemically. The second case showed the mixed features of lymphoepithelioma-like carcinoma and cholangiocarcinoma with predominant lymphoid stroma. In this case, EBV was detected. LELC of hepatobiliary system is an entity distinguished from conventional carcinoma with lymphoid stroma, and its association with EBV warrants further research.


Subject(s)
Bile Duct Neoplasms/diagnosis , Bile Ducts, Intrahepatic , CD8-Positive T-Lymphocytes/pathology , Carcinoma/diagnosis , Cholangiocarcinoma/diagnosis , Herpesvirus 4, Human/isolation & purification , Humans , Liver Neoplasms/diagnosis , Lymphocytes, Tumor-Infiltrating/pathology , Male , Middle Aged , Tomography, X-Ray Computed
6.
Tunisie Medicale [La]. 2007; 85 (8): 688-691
in French | IMEMR | ID: emr-108812

ABSTRACT

The purpose of this work is to study the pathological features and the outcome of lymphoid stroma gastric carcinoma and its relation with Epstein-Bar Virus [EBV] and the importance of the EBV serology in detection of early reccurence. Between January 1990 and december 2004, 155 patients underwent gastric resection for gastric carcinoma. Nine of them had lymphoid stroma gastric carcinoma. Over all survival as well as disease free survival were determined. Detection of reccurence was based on clinical exam and on findings yielded by endoscopy, radiological exams and EBV serology. A comparison of survival rate of patients beteween current gastric carcinoma and those with lymphoid stroma gatsric carcinoma was done using the Log Rank test. the patients [7 men and 2 women] had a mean age of 61 years [41 to 82]. The tumor was in the antrum in 4 cases, in the body of the stomach in 3 cases and in the cardia in 2 cases. The mean tumor size was 8 cm with deep invasion of the stomach wall in 5 cases and ganglion invasion in 6 cases out of 9. EBV was detected in all patients by in situ hybridation. Post operative morbidity and mortality were nil. Global survival and disease free survival were, respectively, 53.3% and 65.6% in lymphoid stroma gatsric carcinoma, and 43.4% and 63.5% for current gatsric carcinoma. Three patients died of a reccurent disease after 6, 30 and 33 months. The remaing 6 patients didn't have signs of reccurence. lymphoid stroma gastric carcinoma is a bulky, local disease. In our patients, it was always associated with EBV. Over all survival was better than survival of current gastric carcinoma, although there was no significance. EBV serology can be an effective screening procedure of reccurence


Subject(s)
Humans , Male , Female , Stomach Neoplasms/pathology , Lymphoid Tissue , Adenocarcinoma/epidemiology , Epstein-Barr Virus Infections , Herpesvirus 4, Human/isolation & purification
7.
Article in English | IMSEAR | ID: sea-37791

ABSTRACT

Epstein-Barr virus (EBV)-encoded small RNA can be detected in about 1-17 % of gastric carcinomas. To elucidate lifestyles and other factors related to such an EBV-associated gastric carcinoma (EBV-GC), we conducted a case-control study in Cali, Colombia. The study subjects were 368 patients with gastric carcinoma newly diagnosed during the period between September 2000 and June 2003, including 42 EBV-GC cases. We obtained information on lifestyles, dietary habits, and occupational exposure by a questionnaire. The frequency of EBV-GC was related to birth order of patients (P for trend =0.025). More precisely, EBV-GC was much less frequent among the patients who were the eldest child in a family (P=0.007). Those findings were contrary to what was reported by the study conducted in Japan, where EBV-GC was more frequently observed among eldest brothers/sisters. A possible explanation for the apparently conflicting results is that EBV-GC risk is related to the age at first EBV infection but its relationship is not monotonic. In addition to the relationship with birth order, the present study showed that high salt intake and metal dust exposure may be related to EBV-GC as reported by the Japanese study although these associations observed in the present study were not statistically significant. No significant association was observed in other factors, including dietary habits. Further studies seem warranted to elucidate the difference between Japan and Colombia with respect to the environmental factors related to EBV-GC cases.


Subject(s)
Aged , Birth Order , Case-Control Studies , Colombia/epidemiology , Diet , Epstein-Barr Virus Infections/complications , Female , Herpesvirus 4, Human/isolation & purification , Humans , Life Style , Male , Middle Aged , Occupational Exposure , Surveys and Questionnaires , Risk Factors , Stomach Neoplasms/epidemiology
8.
Article in English | IMSEAR | ID: sea-38930

ABSTRACT

Lymphoepithelial carcinoma is a relatively uncommon malignant tumor of the salivary gland demonstrating malignant epithelial cells with dense lymphoid stroma. The authors report three cases of lymphoepithelial carcinoma associated with Epstein-Barr virus of the right parotid gland with clinically presenting as painless, gradual enlargement of the preauricular mass. The histopathologic examination of the parotid gland is characterized by malignant epithelial cells with dense lymphoid stroma. Immunohistochemical stains show positive reactivity to cytokeratin and p53 in malignant epithelial cells. In situ hybridization of the Epstein-Barr virus-encoded Ribonucleic acid shows positivity in malignant epithelial cells. Clinical and pathologic features with relevant literatures are discussed. These are the first reported cases of primary parotid lymphoepithelial carcinoma associated with Epstein-Barr virus infection in Thailand and Southeast Asia.


Subject(s)
Adult , Carcinoma, Squamous Cell/pathology , Epstein-Barr Virus Infections/pathology , Female , Herpesvirus 4, Human/isolation & purification , Humans , In Situ Hybridization , Male , Middle Aged , Parotid Neoplasms/pathology , Thailand
9.
Article in English | IMSEAR | ID: sea-37703

ABSTRACT

Epstein-Barr virus (EBV) infection is highly associated with specific subtypes of malignant lymphoma. In our previous report on nodal malignant lymphoma in Thailand, we found that 64% of classical Hodgkin's lymphoma (cHL), 51% of non-Hodgkin's lymphoma, T-cell (NHL-T), and 13% of non-Hodgkin's lymphoma, B-cell (NHL-B) were EBV-related. In the present research, we conducted a retrospective study of primary extranodal non-Hodgkin's lymphoma of the sinonasal tract (e-NHL-ST) and primary extranodal non-Hodgkin's lymphoma of the nasopharynx (e-NHL-NP) in Southern Thailand, between 1997 and 2004. EBV-encoded RNA (EBER) expression by in situ hybridization was performed in all cases and a T-cell receptor (TCR)-g gene rearrangement study was performed in NHL-T cases. There were 18 cases of e-NHL-ST and 42 cases of e-NHL-NP detected by histologic and immunohistochemistry examinations. The percentages of e-NHL-ST and e-NHL-NP as compared to nodal malignant lymphoma were 3.7% and 6.8%, respectively. Sixteen cases (88.9%) of e-NHL-ST and 7 cases (16.7%) of e-NHL-NP were NHL-T, and the remainder were NHL-B. All of the NHL-T cases in both sites were EBER-positive. Two (5.4%) of the NHL-B cases in the nasopharynx showed EBER positive. Monoclonal bands of the TCR-gamma gene were detected in 71.4% of the extranodal NK/T-cell lymphomas, nasal type, patients; 50.0% of peripheral T-cell lymphoma, unspecified, patients; and one case of angioimmunoblastic T-cell lymphoma. This study indicates a very strong association of NHL-T in the sinonasal tract or nasopharynx with EBV infection, the link apparently being weaker in NHL-B patients. The study also indicates that most cases of extranodal NK/T-cell lymphoma, nasal type, are not the germline configuration of the TCR genes.


Subject(s)
Adult , Age Distribution , Aged , Aged, 80 and over , Cohort Studies , DNA, Viral/analysis , Female , Herpesvirus 4, Human/isolation & purification , Humans , In Situ Hybridization , Incidence , Lymph Nodes/pathology , Lymphoma, Non-Hodgkin/epidemiology , Lymphoma, T-Cell/epidemiology , Male , Middle Aged , Nasopharyngeal Neoplasms/epidemiology , Paranasal Sinus Neoplasms/epidemiology , Polymerase Chain Reaction , Prognosis , Retrospective Studies , Risk Assessment , Sex Distribution , Survival Rate , Thailand/epidemiology
10.
Almustansiriya Journal of Pharmaceutical Sciences. 2006; 3 (1): 60-74
in English | IMEMR | ID: emr-75761

ABSTRACT

Malignant lymphomas are among the common tumors that are associated with and may complicate Epstein Barr Virus [EBV] infection. Although the strongest association is with the endemic Burkitt lymphomas [BL], the trend of association with sporadic lymhpomas reveals a consistently increasing prevalence of the virus in Hodgkin's disease [HD] in recent years compared to the non-Hodgkin type [NHL] which may point to a possible role for the virus in the predisposition and etio-pathogenesis of the disease. evaluate the association of EBV with Hodgkin's and non-Hodgkin lymphomas in relation to age, sex and HD subtype retrospectively using archival tissue biopsy sections. EBV was detected by In Situ Hybridization [ISH] using EBERs RNA probes in paraffin-embedded tissue sections prepared from archival tissue biopsy blocks. [a] EBV was detected in 25 of 40 HD cases [62.5%], 9 of 30 [30%] NHL cases, 4 of 10 [40%] BL cases, and in 5 of 20 [25%] other [non-BL] NHL cases, [b] Among the EBV-positive HD cases, 19 [76%] were of the mixed cellularity [MC] subtype, 1 [4%] of the Nodular Sclerosis [NS] subtype, 1 [4%] of the Lymphocyte Predominance [LP] subtype and 4 [16%] cases were of the Lymphocyte Depletion [LD] subtype, [c] Age distribution of HD cases revealed a bi-modal pattern characterized by an early major peak [67.5% of cases] below 35 years and a minor peak [32.5% of cases] above the age of 40. On the contrary, NHL cases revealed a nearly even age distribution [43.3% versus 56.6%] below and above the age of 40, respectively, [d] No difference was observed in the incidence of HD between males and females where the ratio was close to 1:1. On the other hand, a slight male predominance was seen among NHL cases with a male to female ratio of 2:1. [a] the prevalence rate of EBV infection was high among HD cases and fell within the prevalence rates found in previous similar studies revealing a range of values from 20 to 90%. [b] the higher prevalence of EBV positivity in HD compared to NHL found in this study points to a more substantial role for the virus in the pathogenesis of former compared to the latter disease which also comes in agreement with the greater environmental element compared to the genetic element in the etiology of HD. [c] The unexpected high EBV positivity in the LD subtype of HD may be interpreted as result of the progression of some of the early less aggressive MC-HD cases to the advanced more aggressive LD-HD subtype, [d] the bi-modal age distribution of EBV-positive HD cases follows the same pattern of distribution of the disease in general and testifies for the influence of environmental factors in the incidence of the disease


Subject(s)
Humans , Male , Female , Herpesvirus 4, Human/isolation & purification , Prevalence , Lymphoma , Burkitt Lymphoma , Hodgkin Disease , Lymphoma, Non-Hodgkin
11.
Archives de l'Institut Pasteur de Tunis. 2006; 83 (1-4): 41-47
in French | IMEMR | ID: emr-76078

ABSTRACT

The nasopharyngeal carcinoma [NPC] is frequent in Tunisia. It's the second ORL cancer of men after the larynx one. To analyse the NPC characteristics in our population, we determined the frequency of EBV infection in 47 paraffin-embedded and 6 fresh NPC biopsies. We first extracted the DNA from tumoral tissus and then amplified viral sequences by PCR to detect and to type the infecting virus [EBV-A or ABV-B]. Our results showed that amplifiable DNA has been obtained from 34/47 paraffin-embedded NPC biopsies while 13/47 of the others biopsies contained degraded and not amplifiable DNA. All the fresh biopsies allowed to obtain DNA with good quality. The EBV infection frequency in paraffin-embedded NPC biopsies is 35% while EBV is detected in all fresh biopsies [6/6]. Our analyse also showed that the EBV-A is predominant in our population compared to EBV-B as it was shown in most countries of the world. This study clearly shows that PCR results obtained with paraffin-embedded NPC biopsies are divergeant from those obtained with fresh biopsies. Because of DNA degradation in paraffin-embedded NPC biopsies, the biology molecular results from that kind of samples is criticable. Moreover, the results obtained from fresh NPC biopsies confirmed the quasi-constant association of EBV with undifferenciated carcinoma nasopharyngeal type


Subject(s)
Humans , Nasopharyngeal Neoplasms/virology , Herpesvirus 4, Human/isolation & purification , Polymerase Chain Reaction , Herpesvirus 4, Human/pathogenicity , Biopsy , Paraffin
12.
Article in English | IMSEAR | ID: sea-40930

ABSTRACT

Epstein-Barr virus (EBV) is associated with several malignancies including nasopharyngeal carcinoma and lymphoma in immunocompromised patients. Quantitative monitoring of EBV DNA in these patients has recently become essential for management of the disease. In the present study the authors developed a rapid and reliable real-time PCR to quantify the EBV DNA in peripheral blood mononuclear cell (PBMC) using hybridization probe technique. The real-time primers and probes in this real-time PCR system were designed based on EBNA-1 sequence. The newly-established real-time PCR demonstrated its high sensitivity (as few as 10 copies of EBV could be detected) and specificity. The intra- and inter-assay variations of the assay were shown to be within a 0.5-log10-difference range. A total of 2 EBV-seronegative, 14 EBV-seropositive healthy donors and 4 patients with PCNSL were enrolled into the study. Our results revealed the median of EBV-DNA in lymphoma patients (7886 copies/10(6) PBMC or 15,150 copies /microg DNA) was higher than that of healthy donors (<10 copies/l0(6) PBMC or <10 copies/microg DNA) with statistic significance (P < 0.01). Assessment of this assay in larger number of donors and patients will provide clinical cut-off values which are essential for monitoring and diagnosis of EBV-associated diseases.


Subject(s)
Adult , Blood Donors , Case-Control Studies , Computer Systems , DNA, Viral/analysis , Epstein-Barr Virus Infections/complications , Female , Herpesvirus 4, Human/isolation & purification , Humans , Lymphoma, T-Cell/physiopathology , Male , Middle Aged , Reverse Transcriptase Polymerase Chain Reaction/methods , Time Factors
13.
Article in English | IMSEAR | ID: sea-39664

ABSTRACT

BACKGROUND: High frequency of Epstein-Barr virus (EBV) in the normal mucosa of the upper aerodigestive tract suggests that it may serve as a reservoir for the virus. Malignant lymphomas arising in this site may be associated with EBV. OBJECTIVES: To determine the prevalence of EBV infection in extranodal malignant lymphomas of the upper aerodigestive tract. SETTING: King Chulalongkorn Memorial Hospital, Thailand. DESIGN: Descriptive study. PATIENTS: 42 Thai patients who presented between 1998 and 2003. MATERIAL AND METHOD: The expression of EBV mRNAs (EBERs) of malignant lymphoma was studied by means of in situ hybridization in formalin-fixed, paraffin-embedded specimens. RESULTS: The recruited subjects were 26 males and 16 females, and their age ranged from 3 to 85 years with the mean of 51.43 years, in 4 of them human immune deficiency virus (HIV) infection was documented. Ten of 42 cases (23.81%) expressed EBER transcripts and were extranodal NK/T-cell lymphomas, nasal type (7 cases), plasmablastic lymphomas (2 cases) and diffuse large B-cell lymphoma (1 case). Three of 4 cases (75%) of known HIV-seropositive cases were EBV-positive (2 plasmablastic lymphomas and 1 diffuse large B-cell lymphoma). CONCLUSION: In the upper aerodigestive tract, EBV was present in some but not all malignant lymphoma. It was associated with extranodal NK/T-cell lymphoma, nasal type and B-cell lymphoma arising in HIV-infected patients, but it was not found in B-cell lymphoma arising in immunocompetent patients.


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Disease Reservoirs , Epstein-Barr Virus Infections/epidemiology , Female , Herpesvirus 4, Human/isolation & purification , Humans , In Situ Hybridization , Lymphoma/physiopathology , Lymphoma, B-Cell/physiopathology , Lymphoma, T-Cell/physiopathology , Male , Middle Aged , Prevalence , Respiratory System/physiopathology , Risk Factors , Thailand/epidemiology , Upper Gastrointestinal Tract/physiopathology
14.
Article in English | IMSEAR | ID: sea-38619

ABSTRACT

An investigation as to whether any association of pediatric HL in Thailand was likely to be EBV positive was performed on formalin-fixed paraffin embedded tissue sections using in situ hybridization for EBV encoded RNA (EBER) technique. The analysis was performed on 15 cases. They were 11 male and 4 female cases. The subtypes of HL according to WHO classification were nodular lymphocyte predominance in 1 (6.6%), nodular sclerosis in 4 (26.6%), mixed cellularity in 9 (60%) and lymphocyte depletion in 1 (6.6%). EBV encoded RNA by in situ hybridization was demonstrated in 92.8% of classic HL: 3 of 4 (75%) with nodular sclerosis; 9 of the 9 with mixed cellularity (100%) and 1 of 1 (100%) with lymphocyte depletion. Case of nodular lymphocyte predominance was negative for EBV, CD 15 CD 30 and positive for CD 20. CD 15 and CD 30 were positive in 78.6% and 85% respectively for classic HL. Our results suggest a strong association of EBV with pediatric classic HL (92.3%) particularly the mixed cellularity subtype (100%). The result confirms the male predominance in pediatric HL. Mixed cellularity is the most common subtype of HL in our series (60%).


Subject(s)
Adolescent , Child , Child, Preschool , Epstein-Barr Virus Infections/complications , Female , Herpesvirus 4, Human/isolation & purification , Hodgkin Disease/complications , Humans , Immunohistochemistry , In Situ Hybridization , Infant , Male , Risk Factors , Thailand
15.
Indian J Pathol Microbiol ; 2005 Apr; 48(2): 257-9
Article in English | IMSEAR | ID: sea-73072

ABSTRACT

A 48 year old female presented with extensive ulceration of the nasal septum of 8 months duration. Investigations confirmed the local nature of the disease. A biopsy revealed large zones of ischemic necrosis and abnormal lymphoid cells invading vessel walls and glandular structures. Florid squamous metaplasia, and pseudoepitheliomatous hyperplasia of mucosal epithelium mimicked squamous cell carcinoma and necrotising sialometaplasia. Immunohistochemistry and insitu hybridization confirmed the diagnosis of an EBV positive, Nasal NK/T cell lymphoma. A Pubmed/Medline search suggests that this is the first documented case from India.


Subject(s)
Carcinoma, Squamous Cell/diagnosis , Diagnosis, Differential , Epstein-Barr Virus Infections/diagnosis , Female , Herpesvirus 4, Human/isolation & purification , Humans , Killer Cells, Natural/pathology , Lymphoma, T-Cell/diagnosis , Middle Aged , Nose Neoplasms/diagnosis
16.
Indian J Pathol Microbiol ; 2004 Apr; 47(2): 189-94
Article in English | IMSEAR | ID: sea-74394

ABSTRACT

Viral infections have been recognized as an integral part of both graft injury and rejection. On routine histology, viral infections are diagnosed only when fully established, by the presence of viral inclusions or cytopathic effect. Although renal transplants are routinely done in many centres in India, the incidence of viral infections is largely unkown. This study was aimed at detecting 5 viral infections namely, cytomegalovirus (CMV), BK polyoma Virus (BKV), Herpes Simplex Virus1 and 2 (HSV1 and 2) and Epstein Barr Virus (EBV) in renal biopsies from 321 renal allograft patients, using immunohistochemical and electron microscopic studies. Sixty two biopsies were selected from a total of 414 (belonging to 321 patients) for immunostaining on the basis of features suspicious of viral infections in hematoxylin and eosin stained sections. Immunostaining confirmed CMV infection in 8 biopsies, BKV infection in 31 biopsies and HSV1 in only 2 biopsies. HSV2 and EBV were not detected in any biopsy. Two biopsies showing CMV immunopositivity and 5 of BKV were further processed for electron microscopy, which supported the diagnoses. Thus, the study highlights the prevalence of BKV and CMV infections in renal transplant patients having graft dysfunction, to be 9.3% and 1.9%, respectively. Besides, only one case each was diagnosed as CMV infection and BKV infection in routine histopathological reporting, establishing the importance of immunohistochemical studies in early diagnosis of these viral infections.


Subject(s)
BK Virus/isolation & purification , Cytomegalovirus/isolation & purification , Graft Rejection/etiology , Herpesvirus 4, Human/isolation & purification , Humans , Immunohistochemistry , Kidney Diseases/etiology , Kidney Transplantation/adverse effects , Microscopy, Electron , Simplexvirus/isolation & purification , Virus Diseases/etiology
17.
Article in English | WPRIM | ID: wpr-113963

ABSTRACT

BACKGROUND: Epstein-Barr virus (EBV) is associated with various lymphoproliferative disorders and nasopharyngeal carcinoma. Recently, some gastric cancer cells were observed to contain the EBV sequence. We detected EBV in gastric cancer by using PCR to determine the frequency of EBV-associated gastric cancer, and performed immunohistochemical staining for the latent membrane protein (LMP1), p53 and CD44 to investigate the possible mechanism in EBV-associated gastric cancer. METHODS: Eighty-seven formalin-fixed and paraffin-embedded blocks (40 gastric adenocarcinomas, 34 adjacent normal tissues, 13 metastatic lymph nodes) from 40 surgically resected gastric specimens were studied. All patients were diagnosed with gastric cancer at the Kang-Nam St. Mary's Hospital between April 1995 and April 1997. After DNA was extracted from each paraffin block, we performed PCR and immunohistochemical staining for the LMP1, p53 and CD44. RESULTS: EBV was detected in 4 of 40 cases (10%). In 1 of 4 EBV-positive cases, EBV was also detected in a metastatic lymph node. The immunohistochemical staining for the LMP1, p53 and CD44 were negative in all the EBV-positive cancer patients. Of the patients having these cancers, 2 had a poorly differentiated adenocarcinoma with a lymphoepithelioma-like morphology. DISCUSSION: The frequency of EBV-associated gastric cancer is about 10% in Korea. Considering the negative result of the immunohistochemical staining for the LMP1, p53 and CD44, EBV-associated gastric cancer seems to have a different mechanism of tumorigenesis from ordinary gastric cancer or other EBV-associated cancers. This specific mechanism must be determined by further large scale studies.


Subject(s)
Adenocarcinoma/metabolism , Adult , Hyaluronan Receptors/metabolism , Female , Herpesvirus 4, Human/isolation & purification , Humans , Immunohistochemistry , Male , Middle Aged , Polymerase Chain Reaction , Tumor Suppressor Protein p53/metabolism , Stomach Neoplasms/metabolism , Viral Matrix Proteins/metabolism
18.
Indian J Pathol Microbiol ; 2003 Jan; 46(1): 34-6
Article in English | IMSEAR | ID: sea-73655

ABSTRACT

The Epstein Barr Virus (EBV) is one of the viruses implicated in the pathogenesis of malignant tumors. Its association with Burkitt's Lymphoma, nasopharyngeal carcinoma and certain cases of Hodgkin's lymphoma is well known. More recently this virus has been found to be associated with gastric carcinoma. Studies on this association, in our country are lacking. We have studied 37 cases of carcinoma using immunohistochemistry for LMP1 protein. Most workers have found an expression of latency type I antigens in gastric adenocarcinomas, with only a few cases being LMP1 positive. In the 37 cases studied by us, 24.14% of cases were positive for LMP1. LMP1 is known to have oncogenic properties. In addition, it has been demonstrated earlier that the EBV particles in gastric carcinoma are monoclonal. These two factors support a pathogenetic role for EBV in gastric carcinoma.


Subject(s)
Adult , Aged , Female , Herpesvirus 4, Human/isolation & purification , Humans , Male , Middle Aged , Stomach Neoplasms/pathology , Viral Matrix Proteins/analysis
19.
Yonsei Medical Journal ; : 175-182, 2002.
Article in English | WPRIM | ID: wpr-89648

ABSTRACT

Expression of the natural killer (NK) cell antigen CD56 is uncommon in malignant lymphoma, but when it is, it is almost exclusively of the non-B cell lineage and show a preference for the nasal and nasopharyngeal region. T/NK cell lymphoma is known to be aggressive and refractory to treatment. It is highly associated with the Epstein-Barr Virus (EBV), but clinical investigations are rarely reported, that is until recently. We report here, on the clinical features and therapeutic outcomes of patients with T/NK cell lymphomas and its association with EBV. We reviewed fifty-four cases with peripheral T cell lymphomas in the upper aerodigestive tract between Jan. 1987 and Aug. 1998 from the Severance Hospital, Yonsei University College of Medicine. The diagnosis of T/NK cell lymphoma was made according to the expression of the NK cell markers, CD56 antigen and cytoplasmic CD3 epsilon, in tumor specimens, by immunohistochemistry. Epstein-Barr early region (EBER) RNA was detected using in situ hybridization on paraffin-embedded sections. Among the 54 cases with malignant lymphomas occurring in the upper aerodigestive tract, 20 had T/NK cell lymphoma (37%). The primary sites of T/NK cell lymphomas were the nasal cavity, 12 cases (60%), the tonsils, 4 cases (20%), the nasopharynx, 2 cases (10%), and the oropharynx, 2 case (10%). There were no differences between the features, at diagnosis or therapeutic modalities for patients with T/NK cell lymphoma and non-T/NK cell lymphoma. The complete remission rate of T/NK cell lymphomas was lower than non-T/NK cell lymphomas (65% vs 85%, p=0.02). The overall survival of T/NK cell lymphomas was 13 months (1-74 month), which was significantly lower than non-T/NK cell lymphomas [60.6% with a median follow up of 22 months (1-101 month, p=0.02)]. Disease free survival of T/NK cell lymphomas was 22 months (4-66 month), significantly lower than non-T/NK cell lymphomas [73.8% with a median follow up of 22 months (2-95 month), p=0.04]. The overall survival rates for T/NK cell lymphomas were significantly lower than for EBV positive non-T/NK cell lymphomas (p=0.018). EBER RNA was detected in the paraffin-embedded tissue sections of all T/NK cell lymphomas, compared to only 17.6% (6 of 34 cases) for non- T/NK cell lymphomas. In conclusion, as patients with T/NK cell lymphomas showed poor clinical outcomes, and a high association with EBV positivity, clinical trials with more investigational therapeutic strategies, and further research into the relationship of EBV infection with pathogenesis of T/NK cell lymphoma is warranted.


Subject(s)
Adult , Aged , Digestive System Neoplasms/therapy , Female , Herpesvirus 4, Human/isolation & purification , Humans , Killer Cells, Natural , Lymphoma/therapy , Lymphoma, T-Cell/therapy , Male , Middle Aged , Respiratory Tract Neoplasms/therapy , Treatment Outcome
20.
Arq. neuropsiquiatr ; 59(3A): 616-618, Sept. 2001.
Article in Portuguese | LILACS | ID: lil-295921

ABSTRACT

A cerebelite aguda pode ocorrer em associação a infecção pelo vírus da varicela-zoster, enterovirus, caxumba, micoplasma e outros agentes infecciosos. A cerebelite aguda é uma complicação rara da infecção pelo vírus Epstein-Barr (EBV). Relatamos o caso de uma mulher de 21 anos com história de 12 dias de evolução com náuseas, vômitos, ataxia de marcha e membros, tremor cefálico e de membros, opsoclono, mioclonias e rash cutâneo. Sorologia para EBV foi positiva. A infecção pelo EBV, com complicações neurológicas, pode não se apresentar com os sinais e sintomas clássicos da mononucleose infeciosa.


Subject(s)
Humans , Female , Adult , Cerebellar Diseases/virology , Epstein-Barr Virus Infections/diagnosis , Herpesvirus 4, Human/isolation & purification , Acute Disease , Anti-Inflammatory Agents/therapeutic use , Antibodies, Viral/isolation & purification , Cerebellar Diseases/diagnosis , Cerebellar Diseases/drug therapy , Dexamethasone/therapeutic use , Epstein-Barr Virus Infections/drug therapy , Herpesvirus 4, Human/immunology , Inflammation
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