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1.
Pesqui. bras. odontopediatria clín. integr ; 22: e210114, 2022. tab, graf
Article in English | LILACS, BBO | ID: biblio-1365227

ABSTRACT

ABSTRACT Objective To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods The culture of hPDLSCs was established. Cell density was set at 2 × 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (α=0.05). Results At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs.


Subject(s)
Periodontal Ligament/anatomy & histology , Root Canal Filling Materials , Stem Cells/immunology , Cytotoxicity Tests, Immunologic/instrumentation , Dental Cements , Immunologic Tests/instrumentation , Brazil , Cell Count , Analysis of Variance , Endodontics , Primary Cell Culture
2.
Hematol., Transfus. Cell Ther. (Impr.) ; 43(4): 410-416, Oct.-Dec. 2021. tab
Article in English | LILACS | ID: biblio-1350825

ABSTRACT

ABSTRACT Background: The screening of Trypanosoma cruzi-infected blood donors using two serological techniques frequently leads to conflicting results. This fact prompted us to evaluate the diagnostic performance of four "in-house" immunodiagnostic tests and two commercially available enzyme-linked immunosorbent assays (ELISAs). Material and Methods: One hundred and seventy-nine blood donors, whose screening for Chagas disease was doubtful, underwent three in-house ELISAs, one in-house immunoblotting test (TESA-blot), and two commercial ELISAs (bioMérieux and Wiener) in an attempt to define the presence or absence of infection. Simultaneously, 29 donors with previous positive results from three conventional serological tests and 30 donors with constant negative results were evaluated. Results: The ELISA-Wiener showed the highest rate in sensitivity (98.92%) and the ELISA-bioMérieux, the highest specificity (99.45%), followed by the TESA-blot, which showed superior performance, with lower false-negative (2.18%) and false-positive (1.12%) rates. In series, the combination composed of the TESA-blot and ELISA-bioMérieux showed slightly superior performance, with trifunctional protein deficiency (TFP) = 0.01%. Conclusion: Our study confirms the high sensitivity and specificity of commercial kits. To confirm the presence or absence of T. cruzi infection, the combination of TESA-blot and ELISA-bioMérieux may be suggested as the best alternative. Individually, the TESA-blot performed the closest to the gold standard; however, it is not commercially available.


Subject(s)
Humans , Trypanosoma cruzi , Immunologic Tests , Chagas Disease , Blood Donors , Enzyme-Linked Immunosorbent Assay , Immunoblotting
3.
Rev. bras. anal. clin ; 53(3): 285-292, 20210930.
Article in Portuguese | LILACS | ID: biblio-1368685

ABSTRACT

Objetivo: O objetivo deste trabalho fora realizar uma análise comparativa entre 13 diferentes TR (teste rápido) para HIV que possuem registro na Agência Nacional de Vigilância Sanitária (ANVISA). Os dados foram retirados das bulas fornecidas nos sítios eletrônicos dos fabricantes dos TR. Métodos: Neste trabalho comparou-se os TR em relação aos seus parâmetros como sensibilidade, especificidade, acurácia, Valor Preditivo Positivo (VPP) e Valor Preditivo Negativo (VPN) e seus interferentes relatados em suas respectivas bulas. Resultados: Observou-se que somente um fabricante não foi condizente com os valores estipulados pelo Departamento de DST, AIDS e Hepatites Virais (DDAHV) que foi o ECO Teste com valores de sensibilidade e especificidade de 99,1% e 99,2%, respectivamente. Conclusão: Concluiu-se que os fabricantes Alere Determine, Bioclin, Bioeasy, Imunocrom, MedTeste e OnSite revelaram-se com as bulas mais completas, apresentando todos os parâmetros necessários para avaliação do desempenho dos TR em questão.


Objective: The objective of this work was to carry out a comparative analysis between 13 different RT (rapid test) for HIV that are registered with the National Health Surveillance Agency (Anvisa). The data were taken from the package inserts provided on the websites of the manufacturers of the RT. Methods: In this work, the RT in relation to your parameters such as sensitivity, specificity, accuracy, Positive Predictive Value (PPV) and Negative Predictive Value (NPV) and their interferences reported in their respective package inserts was compared. Results: We conclude that only one manufacturer does not comply with the values stipulated by the DDAHV that was the ECO Test with values of sensitivity and specificity of 99,1% and 99,2% respectively. Conclusion: The manufacturers Alere Determine, Bioclin, Bioeasy, Immunocrom, MedTest and OnSite have revealed themselves with the most complete package inserts presenting all the necessary parameters to evaluate the performance of the RT in question.


Subject(s)
Acquired Immunodeficiency Syndrome/diagnosis , HIV , HIV Testing , Immunologic Tests
4.
Rev. epidemiol. controle infecç ; 11(1): 32-39, jan.-mar. 2021. ilus
Article in English | LILACS | ID: biblio-1362159

ABSTRACT

Background and Objectives: A novel type of coronavirus, SARS-CoV-2, is responsible for an unprecedented pandemic with profound socioeconomic consequences. Owing to its recent discovery still represents a great unknown to researchers. Thus, this study aims to establish the spatio-temporal associations of the incidence, mortality, and the rate of both rapid and RT-PCR tests in Minas Gerais. Methods: This is a quantitative analysis of secondary data based on a cross-sectional research design. Incidence, mortality, date of the first notification of COVID-19 and number of rapid and RT-PCR tests were obtained from the sources: "GAL", "e-SUS VE" and "SES-MG". Pearson coefficient for correlation was calculated to establish the level of association between the relevant data. Descriptive statistical procedures were used to provide a comprehensive understanding of the distribution of incidence, mortality and test rates in the territory. Results: Positive correlations were found between the rate of rapid tests and incidence; rate of RT-PCR tests and incidence/mortality. At the municipal level, incidence, mortality, rate of rapid tests and RT-PCR revealed a negative correlation with days elapsed since the First Notified Case. The same effect occurs at the level of health macro-regions. Conclusion: The heterogeneity of the incidence and mortality of COVID-19 in the territory of Minas Gerais, as well as the rate of tests may be caused, in part, due to the different dates of introduction of the virus in the municipalities/macro-regions. It is speculated that this phenomenon occurs due to the dynamics of regional and inter-regional flows of people.(AU)


Justificativa e Objetivos: Um novo tipo de coronavírus, SARS-CoV-2, é responsável por uma pandemia sem precedentes com profundas consequências socioeconômicas. Devido à sua recente descoberta, o vírus surgido na cidade chinesa de Wuhan, em dezembro de 2019, ainda lança grandes incógnitas. Este estudo objetiva estabelecer as associações espaço-temporais da incidência; mortalidade; e taxas de testes rápidos e RT-PCR em Minas Gerais. Métodos: Trata-se de uma análise quantitativa de dados secundários a partir de um desenho de pesquisa transversal. Incidência, mortalidade, data da(s) primeira(s) notificações da doença, número de testes rápidos e de RT-PCR foram obtidos nas fontes: "Gerenciador de Ambiente Laboratorial", "e-sus VE" e SES-MG. O coeficiente de Pearson para correlação foi calculado para estabelecer o nível de associação entre os dados relevantes. Técnicas estatísticas descritivas foram empregadas para compreender a distribuição da incidência, mortalidade e taxas de testes no território. Resultados: Correlações positivas foram encontradas entre taxa de testes rápidos e incidência; taxa de testes RT-PCR e incidência/mortalidade. A nível municipal, incidência, mortalidade, taxa de testes rápidos e de RT-PCR têm correlação negativa com dias transcorridos desde o Primeiro Caso Notificado. O mesmo efeito ocorre, em diferentes intensidades, a nível das macrorregiões de saúde. Conclusão: A heterogeneidade da incidência e mortalidade da COVID-19 no território mineiro, assim como, das taxas de testes (rápidos e RT-PCR) pode ser causada, em parte, devido às diferentes datas de introdução do vírus nos municípios/macrorregiões de saúde. Especula-se que esse fenômeno se deve às dinâmicas dos fluxos regionais e inter-regionais de pessoas.(AU)


Justificación y Objetivos: El SARS-CoV-2 es responsable por una pandemia sin precedentes con profundas consecuencias socioeconómicas. Debido a su reciente descubrimiento, este vírus representa una gran incógnita para los investigadores. Así, este estudio tiene como objetivo establecer las asociaciones espacio-temporales de la incidencia, la mortalidad y la tasa de pruebas rápidas y RT-PCR en Minas Gerais. Métodos: Trata-se de un análisis cuantitativo de datos secundarios basado en un diseño de investigación transversal. Incidencia, mortalidad, fecha de la primera notificación de COVID-19 y número de pruebas rápidas y RT-PCR se obtuvieron de las fuentes: "GAL", "e-SUS VE" y "SES-MG". Se calculó el coeficiente de correlación de Pearson para establecer el nivel de asociación entre los datos relevantes. Se utilizaron procedimientos estadísticos descriptivos para proporcionar una comprensión integral de la distribución de la incidencia, la mortalidad y las tasas de prueba en el territorio. Resultados: Se encontraron correlaciones positivas entre la tasa de pruebas rápidas y la incidencia; tasa de pruebas de RT-PCR y incidencia/mortalidad. A nivel municipal, la incidencia, mortalidad, tasa de pruebas rápidas y RT-PCR revelaron una correlación negativa con los días transcurridos desde el Primer Caso Notificado. El mismo efecto ocorre a nivel de macrorregiones de salud. Conclusiones: La heterogeneidad de la incidencia y mortalidad de COVID-19 en el territorio de Minas Gerais, así como la tasa de pruebas puede deberse, en parte, a las diferentes fechas de introducción de la virus en los territorios. Se especula que este fenómeno ocurre debido a la dinámica de los flujos regionales e interregionales de personas.(AU)


Subject(s)
Humans , Immunologic Tests , Spatio-Temporal Analysis , SARS-CoV-2 , COVID-19/mortality , COVID-19/epidemiology
6.
Pesqui. vet. bras ; 41: e06814, 2021. graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1279534

ABSTRACT

The protozoan Neospora caninum is known worldwide as one of the main causes of abortion in cattle. During infection, rhoptry proteins present in the apical complex of the parasite play important roles in adhesion and parasitophorous vacuole formation. The use of N. caninum ROP2 in experimental vaccines has shown promising protective results. In our study we performed cloning and expression in Escherichia coli of an antigenic portion of N. caninum ROP2. The recombinant protein (rROP2) was obtained in insoluble form, and the purified protein showed a size of approximately 18kDa. Even being a small truncate NcROP2 region, it was possible to conserve the antigenic epitopes which were recognized by bovine serum naturally infected with N. caninum. Vaccination with rROP2 on aluminum hydroxide adjuvant induced high levels of rROP2-specific IgG antibodies capable of recognizing native protein in tachyzoite lysates. In conclusion, our approaches were effective in obtaining the rROP2 protein, which induced specific mouse immune response and was also recognized by sera from N. caninum naturally infected cattle. These results suggest that it is a promising antigen for the development of neosporosis subunit vaccines as well as a suitable antigen for use in immunodiagnosis.(AU)


O protozoário Neospora caninum é conhecido mundialmente como uma das principais causas de aborto em bovinos. Durante a infecção, as proteínas rhoptry presentes no complexo apical do parasita desempenham papel importante na adesão e formação de vacúolos parasitóforos. O uso de ROP2 de N. caninum em vacinas experimentais tem mostrado resultados de proteção promissores. Em nosso estudo, realizamos a clonagem e expressão em Escherichia coli de uma porção antigênica de N. caninum ROP2. A proteína recombinante (rROP2) foi obtida na forma insolúvel, e a proteína purificada apresentou tamanho aproximado de 18kDa. Mesmo sendo uma pequena região truncada de NcROP2, foi possível conservar os epítopos antigênicos que foram reconhecidos pelo soro de bovinos naturalmente infectados com N. caninum. A vacinação com rROP2 adsorvida no adjuvante de hidróxido de alumínio induziu altos níveis de anticorpos IgG anti-rROP2, capazes de reconhecer a proteína nativa em lisados de taquizoítos. Em conclusão, nossas abordagens foram eficazes na obtenção da proteína rROP2, que induziu resposta imune específica em camundongos e também foi reconhecida por soros de bovinos naturalmente infectados com N. caninum. Estes resultados sugerem que rROP2 é um antígeno promissor para o desenvolvimento de vacinas de subunidades de neosporose, bem como um antígeno adequado para uso em imunodiagnóstico.(AU)


Subject(s)
Immunologic Tests , Immunoglobulin G , Vaccines , Neospora , Cloning, Organism
7.
Braz. arch. biol. technol ; 64(spe): e21200147, 2021. tab, graf
Article in English | LILACS | ID: biblio-1285565

ABSTRACT

Abstract With the COVID-19 pandemic, many diagnostic tests (molecular or immunological) were rapidly standardised, given the urgency of the situation, many are still in the process of being validated. The main objective of this study was to review the aspects of the diagnostic kits approved in Brazil and their application in the different federative units to gather epidemiological information. In order to achieve these objectives, a survey was carried out on the data available at the regulatory agency (ANVISA) and in the literature. The main countries that have registered products in Brazil are China (51.4%), Brazil (16.6%), South Korea (9.2%), USA (8.8%) and Germany (3.6%). The methodologies of these products are based on the detection of nucleic-acid (15.8%), antigen (13%) and antibody (71.2%). In the immunological tests, it was verified that the sensitivity ranged from 55 to 100% and the specificity from 80 to 100%. The percentage of cases in the samples tested in Brazil is elevated in almost all federative units since eight states showed 40% of positive cases in tested samples, while 18 states displayed between 20 and 40%. In conclusion, this review showed that Brazil is dependent on external technology to respond to pandemics, epidemics and endemics disease and needs to improve its biotechnological scheme to solve further diseases outbreaks.


Subject(s)
Humans , SARS Virus/isolation & purification , COVID-19/diagnosis , Immunologic Tests/instrumentation , Brazil/epidemiology , Enzyme-Linked Immunosorbent Assay/instrumentation , Chromatography, Affinity/instrumentation , COVID-19 Testing/instrumentation , COVID-19 Nucleic Acid Testing/methods
8.
Lima; Perú. Ministerio de Salud; 20200600. 9 p.
Monography in Spanish | MINSAPERU, LILACS, MINSAPERU | ID: biblio-1102298

ABSTRACT

El documento contiene el procedimiento para el control y vigilancia del ingreso, distribución, comercialización, uso, registro de resultados y control de calidad de los dispositivos de diagnóstico in vitro: pruebas rápidas y moleculares para el COVID-19.


Subject(s)
Immunologic Tests , Guidelines as Topic , Quality Management , COVID-19
9.
Lima; Perú. Ministerio de Salud; 20200400. 17 p.
Monography in Spanish | MINSAPERU, LILACS, MINSAPERU | ID: biblio-1095614

ABSTRACT

El documento contiene las consideraciones éticas para la investigación en salud con seres humanos basadas en estándares internacionales sobre ética de la investigación con seres humanos recogidos en las Pautas éticas internacionales para investigación.


Subject(s)
Immunologic Tests , Humans , Biomedical Research , Codes of Ethics
11.
Lima; Perú. Poder Ejecutivo; 20200300. 3 p.
Non-conventional in Spanish | MINSAPERU, LILACS, MINSAPERU | ID: biblio-1116591

ABSTRACT

El Decreto contiene las medidas adicionales extraordinarias que permitan adoptar las acciones preventivas y de respuesta para reducir el riesgo de propagación y el impacto sanitario de la enfermedad causada por el virus del COVID-19, en el territorio nacional, con la finalidad de reforzar los sistemas de prevención, control, vigilancia y respuesta sanitaria, preservar la salud y el empleo de los trabajadores; y de esta forma coadyuvar a disminuir la afectación de la economía peruana por la propagación del mencionado virus a nivel nacional.


Subject(s)
Immunologic Tests , Surveillance , Decrees , Diagnosis , Control , COVID-19
12.
São Paulo; s.n; 2020. 165 p. ilus.
Thesis in Portuguese | SES-SP, LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-TESESESSP, SES-SP | ID: biblio-1368422

ABSTRACT

Angiostrongylus cantonensis é o agente etiológico da neuroangiostrongilíase, o qual se desenvolve em diversas espécies de moluscos e atinge a fase adulta em vertebrados, principalmente roedores, com a possibilidade de participação de hospedeiros paratênicos. A transmissão humana ocorre por meio da preparação inadequada e consumo de moluscos ou hospedeiros de transporte infectados, onde a migração de larvas L3 para o sistema nervoso central causa o quadro de meningite eosinofílica. O diagnóstico laboratorial da patologia é baseado em testes moleculares, que podem apresentar baixa sensibilidade em regiões não endêmicas, e imunológicos utilizando antígenos brutos de fêmeas jovens e adultas, com sensibilidades e especificidades variadas. O objetivo deste trabalho foi estudar diferentes preparações antigênicas de fases evolutivas distintas de A. cantonensis, com o intuito de aperfeiçoar as técnicas sorológicas para o imunodiagnóstico da doença. Para tanto, fêmeas adultas foram avaliadas pela reação de imunofluorescência indireta em cortes parafinados para a focalização de regiões antigênicas no corpo do parasita. Além disso, frações antigênicas de diferentes formas evolutivas foram avaliadas pelas técnicas Dot-ELISA e Western blot com soros e LCRs. Os resultados apontam para a presença de proteínas antigênicas no sistema reprodutivo de fêmeas, além da possibilidade de melhoria do diagnóstico com a utilização de antígenos brutos de fêmeas em associação com antígenos alcalinos de membrana extraídos de vermes adultos para auxiliar no diagnóstico e desencadear ações de vigilância e controle da angiostrongilíase meningoencefálica.


Subject(s)
Immunologic Tests , Angiostrongylus cantonensis , Meningitis
13.
Article in English | WPRIM | ID: wpr-876113

ABSTRACT

@#We characterize the clinical and laboratory characteristics of 5 patients with Graves’ thyrotoxicosis whose serum free thyroxine (fT4) concentration decreased unexpectedly to low levels on conventional doses of carbimazole (CMZ) therapy. The initial fT4 mean was 40.0 pM, range 25-69 pM. Thyroid volume by ultrasound measured as mean 11 ml, range 9.0-15.6 ml. Initial TSI levels measured 1487% to >4444%. Serum fT4 fell to low-normal or hypothyroid levels within 3.6 to 9.3 weeks of initiating CMZ 5 to 15 mg daily, and subsequently modulated by fine dosage adjustments. In one patient, serum fT4 fluctuated in a “yo-yo” pattern. There also emerged a pattern of low normal/low serum fT4 levels associated with discordant low/mid normal serum TSH levels respectively, at normal serum fT3 levels. The long-term daily-averaged CMZ maintenance dose ranged from 0.7 mg to 3.2 mg. Patients with newly diagnosed Graves' hyperthyroidism who have small thyroid glands and markedly elevated TSI titres appear to be “ATD dose sensitive.” Their TFT on ATD therapy may display a “central hypothyroid” pattern. We suggest finer CMZ dose titration at closer follow-up intervals to achieve biochemical euthyroidism.


Subject(s)
Carbimazole , Immunoglobulins, Thyroid-Stimulating , Immunoglobulins, Thyroid-Stimulating , Immunologic Tests , Graves Disease
14.
Mem. Inst. Oswaldo Cruz ; 115: e200201, 2020. tab
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1135281

ABSTRACT

Angiostrongylus costaricensis is the causative agent of abdominal angiostrongyliasis, a zoonotic infection that may produce severe eosinophilic enterocolitis or hepatitis in humans. Parasites are usually not released in stools and serology has an important role in diagnosis. Since cross-reactivity is demonstrated between A. costaricensis and another metastrongylid worm, A. cantonensis, we tested heterologous recombinant galectin as a probe in an immunochromatographic rapid diagnostic test (ICT-RDT) for detection of anti-A. costaricensis antibodies. Almost all (11/12) positive control sera from A. costaricensis infected patients were positive at ICT RDT. These are preliminary indications that r-galectin ICT-RDT is useful for diagnosing A. costaricensis infection.


Subject(s)
Humans , Animals , Strongylida Infections/diagnosis , Angiostrongylus cantonensis , Angiostrongylus , Immunologic Tests , Immunoassay
15.
Mem. Inst. Oswaldo Cruz ; 115: e200287, 2020. tab, graf
Article in English | LILACS | ID: biblio-1154869

ABSTRACT

BACKGROUND The heat-labile nature of Dengue virus (DENV) in serum samples must be considered when applying routine diagnostic tests to avoid issues that could impact the accuracy of test results with direct implications for case management and disease reporting. OBJECTIVES To check if pre-analytical variables, such as storage time and temperature, have an impact on the accuracy of the main routine diagnostic tests for dengue. METHODS Virus isolation, reverse transcription real-time polymerase chain reaction (RT-PCR) and NS1 enzyme-linked immunosorbent assay (ELISA) were evaluated using 84 samples submitted to different pre-analytical conditions. FINDINGS Sensitivity and negative predictive value were directly affected by sample storage conditions. RT-PCR and virus isolation showed greater dependence on well-conserved samples for an accurate diagnosis. Interestingly, even storage at -30ºC for a relatively short time (15 days) was not adequate for accurate results using virus isolation and RT-PCR tests. On the other hand, NS1 ELISA showed no significant reduction in positivity for aliquots tested under the same conditions as in the previous tests. MAIN CONCLUSIONS Our results support the stability of the NS1 marker in ELISA diagnosis and indicate that the accuracy of routine tests such as virus isolation and RT-PCR is significantly affected by inadequate transport and storage conditions of serum samples.


Subject(s)
Humans , Immunologic Tests/methods , Enzyme-Linked Immunosorbent Assay/methods , Viral Nonstructural Proteins/immunology , Reverse Transcriptase Polymerase Chain Reaction/methods , Dengue/diagnosis , Dengue Virus/isolation & purification , Antigens, Viral/blood , Predictive Value of Tests , Sensitivity and Specificity , Viral Nonstructural Proteins/genetics , Dengue/blood , Dengue/virology , Dengue Virus/genetics , Dengue Virus/immunology , Antibodies, Viral/blood , Antigens, Viral/immunology
16.
Rio de Janeiro; s.n; ilus; 2020. 75 p. ilus.
Thesis in Portuguese | LILACS | ID: biblio-1252411

ABSTRACT

Testes diagnósticos práticos, rápidos, de fácil operacionalidade e com potencial para formato point of care (POC) são necessários para a melhor eficácia do controle da TB, principalmente em populações negligenciadas ou de difícil manejo diagnóstico como a indígena. A organização mundial da saúde (OMS) divulgou perfis de produtos-alvo de alta prioridade (TPPs) para auxiliar no diagnóstico de TB: TPP1- teste não baseado em escarro, capaz de detectar TB através da identificação de biomarcadores com alta especificidade (Sp) ≥98% e sensibilidade (S) >65 %; TPP2- teste de triagem para identificar pacientes de risco, que apresentem sintomas de TB, ou descartar a doença ou indicar para testes confirmatórios (Sp ≥70% e S ≥90). Os testes sorológicos são atrativos pelo potencial em preencher estas características, mas devido a heterogeneidade da resposta imune entre os pacientes é necessário explorar novos biomarcadores para adequar sua sensibilidade e/ou especificidade aos perfis TPP. Assim, este estudo propõe avaliar biomarcadores micobacterianos como produtos alvos auxiliares no diagnóstico da TB. As proteínas recombinantes PPE59 e a quimera F1 (MT10.3:MPT64) foram purificadas, padronizadas e ensaiadas para a detecção de IgA em soro por meio da técnica do ensaio imunoenzimático (ELISA). Soros de 113 indivíduos de origem indígena, dos quais 37 (33%) tinham TB pulmonar confirmada e 76 (67%) eram indivíduos sadios, foram testados.


Os resultados das sorologias foram analisados isoladamente e em combinação entre si e com os resultados da microscopia em escarro (SM). A maioria dos indivíduos (62%) são do sexo masculino e 22 (59,5%) dos pacientes TB resultaram SM+ . A sensibilidade no ELISA IgA F1 (81%) foi superior ao do PPE59 (37,8%, p = 0.0003) e da SM (59%, p=0,04), ambos ELISAs obtiveram especificidades moderadas (76,3% x 89,5%, respectivamente). A combinatória dos resultados dos ELISA IgA F1 e PPE 59 não adicionou acurácia ao teste (S = 81%, Sp = 72,3%), mas com a adição dos resultados da SM houve um pequeno aumento na sensibilidade do ELISA IgA F1 (83,8 %) e moderado para PPE59 (70,2%). O ELISA IgA F1 identificou todos, exceto um (21/22, 95,4 %) dos casos SM+ , e 60% (9/15) dos casos SM- , enquanto PPE59 45% (10/22) e 20% (4/15), respectivamente. Portanto, o ELISA IgA F1, embora não perfeitamente, tem potencial para ser validada como um WHO-TPP adicional na identificação de pacientes suspeitos de TB em população indígena. (AU)


Subject(s)
Humans , Immunologic Tests , Recombinant Proteins , Indigenous Peoples , Mycobacterium tuberculosis
17.
Braz. arch. biol. technol ; 63: e20190148, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132220

ABSTRACT

Abstract Recombinant proteins are a suggested alternative for the diagnosis of toxocariasis. The current Escherichia coli recombinant protein overexpression system usually produces insoluble products. As an alternative, yeast such as Pichia pastoris have secretory mechanisms, which could diminish the cost and time for production. This study aimed to produce recombinant proteins in Pichia pastoris and verify their sensibility and specificity in an indirect ELISA assay. Two sequences (rTES-30 and rTES-120) of Toxocara canis excretory-secretory antigens were cloned in a pPICZαB vector and expressed in P. pastoris KM71H. Sera samples collected from human adults infected by Toxocara spp. were tested by indirect ELISA using rTES-30 and rTES-120 as antigens. Recombinant proteins were detected at 72 hours after induction, in the supernatant, as pure bands between 60~70 kDa with hyperglycosylation. Regarding diagnosis potential, recombinant antigens had high specificity (95.6%); however, sensitivity was 55.6% for rTES-30 and 68.9% for rTES-120. Further deglycosylation of the P. pastoris antigens did not seem to affect ELISA performance (p>0.05). The low sensitivity in the serodiagnosis diminished any advantage that P. pastoris expression could have. Therefore, we do not recommend P. pastoris recombinant TES production as an alternative for the diagnosis of toxocariasis.


Subject(s)
Humans , Pichia/immunology , Recombinant Proteins/blood , Toxocariasis/diagnosis , Immunologic Tests , Enzyme-Linked Immunosorbent Assay , Sensitivity and Specificity
18.
Rev. Inst. Adolfo Lutz ; 78: 1-9, dez. 2019. graf
Article in Portuguese | ColecionaSUS, LILACS, ColecionaSUS, SES-SP, CONASS, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: biblio-1147471

ABSTRACT

Oitenta porcento dos casos de paracoccidioidomicose (PMC) ocorrem no Brasil. As regiões brasileiras com maior número de casos são: sul, sudeste e centro-oeste, sendo emergente no norte e nordeste. A imunodifusão dupla em gel de agarose assume grande importância no diagnóstico, por permitir o monitoramento da doença e por oferecer subsídios para levantamentos soroepidemiológicos. O objetivo deste trabalho foi de avaliar e caracterizar os pacientes atendidos no Laboratório de Imunodiagnóstico das Micoses do Instituto Adolfo Lutz de São Paulo, em 2016. Trata-se de um estudo retrospectivo realizado utilizando-se dados secundários e avaliando-se as seguintes informações: idade, sexo, procedência do pedido médico, resultado e histórico sorológico dos pacientes. Dos 1.408 pacientes, 12,8% apresentaram reatividade sorológica para Paracoccidioides brasiliensis. Destes, 42,5% não possuiam histórico sorológico, sendo considerados como casos novos da doença. A classificação dos pacientes reagentes por gênero demonstrou que 83,4% eram do sexo masculino, com razão de masculinidade de 5:1. A faixa etária variou de um (1) a 92 anos, e aproximadamente 40% dos pacientes eram da faixa etária de 41 a 60 anos. Este estudo demonstra e reforça a importância da implementação dos estudos soroepidemiológicos como ferramenta auxiliar para nortear as ações de vigilância e políticas em saúde na PCM. (AU)


Eighty percent of paracoccidioidomycosis (PMC) cases occur in Brazil. The highest numbers occur in south, southeast and center-west region, being emergent in the north and northeast areas. The double immunodiffusion in agarose gel is valuable for its diagnosis, as it allows the monitoring of the disease and offers subsidies for the seroepidemiological surveys. This study evaluated and characterized the patients attended in 2016 at the Mycoses Immunodiagnosis Laboratory of Adolfo Lutz Institute of São Paulo. This retrospective study, based on the secondary data, evaluated the information: age, sex, medical request origin, result and serological history of the patients. Of 1,408 patients, 12.8% presented positive serological reactivity for Paracoccidioides brasiliensis. Of them, 42.5% had no serological history and they were considered as new cases. The classification of reactive patients by gender showed that 83.4% were males, being the masculinity ratio of 5:1. The age range was one (1) to 92 years old, and ±40% of the patients were of age ranging from 41-60 years old. This study reinforces the importance of the implementation of the seroepidemiological studies as to guide the surveillance actions and the public health politics in PCM. (AU)


Subject(s)
Paracoccidioides , Paracoccidioidomycosis , Patients , Immunologic Tests
20.
Korean Journal of Medicine ; : 358-361, 2019.
Article in Korean | WPRIM | ID: wpr-759947

ABSTRACT

Pharyngitis is a prevalent disease of the upper respiratory tract that requires treatment with an antibiotic. Group A streptococci (GAS) are the most frequent etiologic agents of bacterial pharyngitis. Because GAS are susceptible to penicillin, routine antibiotic susceptibility testing is not needed. Generally, patients with bacterial pharyngitis have high fever, cervical lymphadenopathy and tenderness, and tonsillar exudative discharge without symptoms of the common cold (e.g., cough, rhinorrhea, and sneezing). However, differentiating bacterial pharyngitis from viral pharyngitis based only on their clinical manifestations is problematic. Therefore, a bacterial culture or a rapid antigen detection test (RADT) is required for the diagnosis of bacterial pharyngitis. Although bacterial culture is the gold standard for diagnosis of bacterial pharyngitis, its accuracy is affected by the technical expertise of the technician, and there is a delay of 1–2 days before the results become available. In contrast, the sensitivity of RADT has increased to over 90%, making them suitable for screening purposes. The result of a RADT is available within 5–10 minutes, obviating the need for a second visit to obtain the results of culture. Use of a RADT would enable the optimal antibiotic to be administered earlier, reducing the overuse of antibiotics.


Subject(s)
Anti-Bacterial Agents , Common Cold , Cough , Diagnosis , Drug Resistance , Fever , Humans , Immunologic Tests , Lymphatic Diseases , Mass Screening , Penicillins , Pharyngitis , Professional Competence , Respiratory System
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