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1.
Article in Chinese | WPRIM | ID: wpr-921824

ABSTRACT

The effect of parasitic ions on the results of ultraviolet A (UVA) cross-linking in iontophoresis was still not clear. In this work, the porcine sclera was cross-linked by riboflavin lactate Ringer's solution (group A) and riboflavin normal saline (group B)


Subject(s)
Animals , Collagen , Cross-Linking Reagents , Ions , Iontophoresis , Permeability , Photosensitizing Agents/pharmacology , Riboflavin , Sclera , Swine , Ultraviolet Rays
2.
Chinese Journal of Biotechnology ; (12): 4015-4023, 2021.
Article in Chinese | WPRIM | ID: wpr-921482

ABSTRACT

Metal-organic frameworks (MOFs) are formed by self-assembly of metal ions or clusters with organic ligands, and are widely used in the fields of catalysis, sensing, energy and biomedicine. Recently, biological composites based on MOFs have attracted increasing attention. MOFs can be used as a platform for encapsulating bioactive substances due to the advantages such as large pore capacity, large specific surface area and diverse structure composition. These features can protect bioactive substances from adverse conditions, e.g. high temperature, high pressure, and organic solvents, thus improving the anti-adversity of bioactive substances. This review summarizes the advances of using MOFs as protective coatings to improve the anti-adversity of different bioactive substances, and introduces the synthesis strategy of MOFs-based biological composites, with the aim to promote the practical application of MOFs-based biological composites.


Subject(s)
Catalysis , Ions , Metal-Organic Frameworks , Metals
3.
Article in Chinese | WPRIM | ID: wpr-879020

ABSTRACT

To demonstrate the fragmentation patterns of simple coumarins furanocourmarin(C_7-C_8), furanocourmarin(C_6-C_7) and dihydrofuran coumarin by mass spectrometry, with fraxin, scopoletin, isopsoralen, pimpinellin, isoimperatorin, notopterol and noda-kenin as study subjects, so as to provide a basis for rapid identification of compounds in different subtypes of coumarins. Ultrahigh performance liquid chromatography combined with quardrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) was implemented in both positive and negative ion modes. Masslynx software was employed to provide the elemental constituents of each detected ion based on its accurate molecular weight. Chemdraw 2014 was used to cultivate mass number of each inferred structure. The fragment pattern of each compound was determined based on the structures inferred from all the relevant ions. And the patterns were drawn by Chemdraw 2014. The deviation between the calculated molecular weight of the inferred structure and the detected value of the ions was used to assess the correctness of the inferred structures in the fragmentation patterns. The results showed that with UPLC-Q-TOF, neutral loss of CO_2 and CO was reflected in lactone and furan skeletons from the courmarin structure. An even mass was attributed to the loss of an odd number of methyl radicals from compounds with a methoxy substituent. Furanocourmarin(C_7-C_8) produced a protonated molecular ion([M+H]~+), while the other courmarin subtypes produced either a sodium adduct of the molecular ion([M+Na]~+) or a sodium adduct of the molecular ion([M+Na]~+) with a protonated molecular ion([M+H]~+). The m/z 203.03 was a diagnostic ion for furanocourmarin(C_6-C_7), and the m/z 147.04 was supplementary evidence for furanocourmarin(C_6-C_7) identification. The characteristic ion of furanocourmarin(C_7-C_8) was m/z 131.05, while m/z 187.04 was the characteristic ion of dihydrofuran coumarin. The m/z 203.03 ion for furanocourmarin(C_7-C_8) was pretty weak. In negative ion mode, furanocourmarin(C_7-C_8) did not have any signals that were different from the other subtypes of courmarins. The fragmentation patterns in negative ion mode for the other subtypes of courmarins were similar to those in positive ion mode. Four types of fragmentation patterns were identified as forcourmarins from Notopterygium inchum. This study provides the basis for the rapid identification of courmarin subtypes by mass spectrometry.


Subject(s)
Chromatography, High Pressure Liquid , Chromatography, Liquid , Coumarins , Humans , Ions , Mass Spectrometry , Plant Extracts , Spectrometry, Mass, Electrospray Ionization
4.
Chonnam Medical Journal ; : 20-26, 2020.
Article in English | WPRIM | ID: wpr-787278

ABSTRACT

We examined the effect of fluoxetine, a selective serotonin reuptake inhibitor antidepressant, on neuronal viability in mouse cortical near-pure neuronal cultures. Addition of fluoxetine to the media for 24 hours induced neuronal death in a concentration-dependent manner. To delineate the mechanisms of fluoxetine-induced neuronal death, we investigated the effects of trolox, cycloheximide (CHX), BDNF, z-VAD-FMK, and various metal-chelators on fluoxetine-induced neuronal death. Neuronal death was assessed by MTT assay. The addition of 20 µM fluoxetine to the media for 24 hours induced 60–70% neuronal death, which was associated with the hallmarks of apoptosis, chromatin condensation and DNA laddering. Fluoxetine-induced death was significantly attenuated by CHX, BDNF, or z-VAD-FMK. Treatment with antioxidants, trolox and ascorbate, also markedly attenuated fluoxetine-induced death. Interestingly, some divalent cation chelators (EGTA, Ca-EDTA, and Zn-EDTA) also markedly attenuated the neurotoxicity. Fluoxetine-induced reactive oxygen species (ROS) generation was measured using the fluorescent dye 2′,7′-dichlorofluorescin diacetate. Trolox and bathocuproine disulfonic acid (BCPS), a cell membrane impermeable copper ion chelator, markedly attenuated the ROS production and neuronal death. However, deferoxamine, an iron chelator, did not affect ROS generation or neurotoxicity. We examined the changes in intracellular copper concentration using a copper-selective fluorescent dye, Phen Green FL, which is quenched by free copper ions. Fluoxetine quenched the fluorescence in neuronal cells, and the quenching effect of fluoxetine was reversed by co-treatment with BCPS, however, not by deferoxamine. These findings demonstrate that fluoxetine could induce apoptotic and oxidative neuronal death associated with an influx of copper ions.


Subject(s)
Animals , Antioxidants , Apoptosis , Brain-Derived Neurotrophic Factor , Cell Death , Cell Membrane , Chelating Agents , Chromatin , Copper , Cycloheximide , Deferoxamine , DNA , Fluorescence , Fluoxetine , Ions , Iron , Mice , Neurons , Reactive Oxygen Species , Serotonin
5.
Chinese Journal of Biotechnology ; (12): 899-907, 2020.
Article in Chinese | WPRIM | ID: wpr-826886

ABSTRACT

Stearoyl-CoAdesaturase-1 (SCD-1) is a key regulator of monounsaturated fatty acid synthesis. It plays a vital role in lipid synthesis and metabolism. Ca²⁺ is an important cation in the body and plays an important role in the organism. The aims of this study were to investigate the correlation of SCD-1 gene overexpression with lipid indexes and calcium ion level. The pcDNA3.1 (+) + SCD-1 +Flag eukaryotic expression vector and cultured duck uterine epithelial cells were co-transfected. The overexpression of SCD-1 gene was measured using the Flag Label Detection Kit. Ca ions and lipid contents were detected through Fluo-3/AM Calcium Ion Fluorescence Labeling method and Lipid Measuring Kit, respectively. SCD-1 gene overexpression was negatively correlated with triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C), and positively correlated with Ca ion, total cholesterol (TC), very low-density lipoprotein cholesterol (VLDL-C) and low density lipoprotein cholesterol (LDL-C) levels. Meanwhile, Ca ion was positively correlated with TG, LDL-C and HDL-C contents, and negatively correlated with TC and VLDL-C levels. Overexpression of SCD-1 gene could regulate Ca ion secretion, as well as lipid synthesis and transport in duck uterine epithelial cells.


Subject(s)
Animals , Calcium , Metabolism , Coenzyme A Ligases , Genetics , Ducks , Epithelial Cells , Chemistry , Gene Expression , Ions , Lipids , Genetics , Triglycerides , Metabolism
6.
Rev. Inst. Adolfo Lutz ; 78: 1-5, dez. 2019. graf, tab
Article in Portuguese | ColecionaSUS, LILACS, ColecionaSUS, SES-SP, CONASS, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO | ID: biblio-1253835

ABSTRACT

Esta nota apresenta a validação de um método para realizar a determinação de lítio em concentrações menores do que 40 µg L­1 em amostras de águas de abastecimento público, utilizando­se cromatografia de íons e calibração externa, com a curva analítica obtida por regressão linear (mínimos quadrados ordinários). O método é seletivo, e apresenta limite de detecção igual a 1,0 µg L­1e limite de quantificação igual a 2,0 µg L­1.Os ensaios de recuperação em três níveis de concentração apresentaram resultados entre 99,4 e 101,9%. Na avaliação da precisão nos mesmos três níveis de concentração, os coeficientes de variação exibiram valores entre 1,1 e 4,0%. (AU)


This note presents the validation of a method for determining the lithium at concentrations less than 40 µg L­1 in the public water supply, by using the ion chromatography and external calibration, and the analytical curve was obtained by the linear regression (ordinary least squares). The employed method is selective, showing the detection limit equal to 1.0 µg L­1 and the quantification limit equal to 2.0 µg L­1. Recovery tests in three concentration levels presented results from 99.4 to 101.9%. On the precision evaluation in the same three concentration levels, the coefficients of variation exhibited values between 1.1 and 4.0%. (AU)


Subject(s)
Water Supply , Chromatography , Ions , Lithium
7.
Odovtos (En línea) ; 21(1): 83-93, Jan.-Apr. 2019. tab, graf
Article in English | LILACS, BBO | ID: biblio-1091473

ABSTRACT

Abstract 20. Conventional glass ionomer cements are used as dental provisional restorative materials, which present several advantages such as adhesion to the tooth mineral phase among others. On the other hand, the knowledge about biological property of glass ionomers shows various approaches and results. In this work, it was studied the in vitro biological response of human gingival fibroblasts in contact with commercial cements of glass ionomer: Mirafil® and Ionglass® and with their extracts, according to ISO 10993. The extracts of the cements, in which the cells were cultured, were adjusted at different concentrations ranging 0.1% to 100%. The cellular metabolic activity of gingival fibroblasts was measured using the Alamar Blue® reagent. The results showed a significant effect on the cellular metabolic activity correlated with the concentration of liberated ions (Al³+ and Ca²+) for both ionomers, as well as the pH variations of the culture media. This could mean that the cellular metabolic activity is substantially influenced by ions and pH of the cell culture.


Resumen 24. Los cementos de ionómero de vidrio convencionales se utilizan como materiales de restauración provisional para uso dental, los cuales presentan varias ventajas como la adhesión a la fase mineral de los dientes. Por otro lado, las propiedades biológicas de los ionómeros de vidrio muestran diversos enfoques y resultados. En éste trabajo se estudió la respuesta biológica in vitro de fibroblastos gingivales humanos en contacto con cementos comerciales de ionómero de vidrio: Mirafil® e Ionglass® y con sus respectivos extractos según la norma ISO 10993. Los extractos de los cementos en los que se cultivaron las células estaban en diferentes concentraciones: de 0.1% a 100%. La actividad metabólica celular se midió usando el reactivo Alamar Blue®. Los resultados mostraron un efecto significativo sobre la actividad metabólica celular correlacionada con la concentración de iones liberados (Al³+ y Ca²+) para ambos ionómeros, así como las variaciones de pH de los medios de cultivo. Ello podria explicar la influencia por los iones y el pH del cultivo celular en la actividad metabólica celular.


Subject(s)
Dental Cementum , Dental Restoration, Temporary , Glass Ionomer Cements/analysis , Cell Survival , Ions
8.
Pesqui. bras. odontopediatria clín. integr ; 19(1): 4546, 01 Fevereiro 2019. tab, ilus
Article in English | LILACS, BBO | ID: biblio-998216

ABSTRACT

Objective: To assess the levels of nickel and chromium ions in hair and Gingival Crevicular Fluid (GCF) of orthodontic patients and to evaluate the corrosion of orthodontic bracket surfaces. Material and Methods: Nickel and chromium ion concentrations were measured in hair and GCF of 15 patients (9 females and 6 males, aged 16-28 years old) who had fixed orthodontic treatment using atomic absorption spectroscopy. The samples were taken before treatment (baseline), 4, 8, and 16 months later during treatment. Along with ionic sampling, microscopic sampling was done. One of each patient brackets was removed to get 15 brackets per group. Five brackets were taken randomly from each group to be examined under scanning electron microscope (SEM). Data obtained were analyzed using paired t-tests. Results: After 16 months, compared with the baseline, average hair nickel level changed from 0.125 µg/g to 0.956 µg/g with statistically significant difference (p=0.00); average chromium level changed from 0.090 µg/g to 0.295 µg/g but no significant difference (p>0.05); average GCF nickel level changed from 3.335 µg/g to 10.410 µg/g; average chromium level changed from 1.859 µg/g to 9.818 µg/g. Both of these increases were significant (p=0.000). SEM examinations showed that the corrosion on brackets was seen in the fourth month, and more severely visible after 8 and 16 months of uses. Conclusion: After 16 months of treatment, compared with the baseline, the hair nickel level was increased by 7.7 times; while for chromium was by 3.3 times. Gingival crevicular fluid nickel level was increased by 3.1 times and chromium level was by 5.3 times. The longer time of treatment, the more ions released and the more corrosion of brackets will be.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Orthodontics , Longitudinal Studies , Chromium , Dental Etching/methods , Nickel , Indonesia , Ions
9.
Yonsei Medical Journal ; : 751-759, 2019.
Article in English | WPRIM | ID: wpr-762110

ABSTRACT

PURPOSE: This study aimed to explore the effects and mechanisms of long non-coding RNA (lncRNA) anti-differentiation non-coding RNA (ANCR) on the osteogenesis of osteoblast cells in postmenopausal osteoporosis (PMOP). MATERIALS AND METHODS: Mice models of PMOP were established. ANCR expression and intracellular calcium ions were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and laser confocal microscopy, respectively. ANCR was silenced in osteoblast cells from PMOP mice by the transfection of siRNA-ANCR (si-ANCR). The proliferation and apoptosis of osteoblast cells was analyzed by MTT and flow cytometry, respectively. Alkaline phosphatase (ALP) activity and calcium nodules were examined by ALP and alizarin red staining assay, respectively. The expression of enhancer of zeste homolog 2 (EZH2), runt related transcription factor 2 (RUNX2), and OSTERIX was detected by qRT-PCR and Western blot. Furthermore, an osteogenesis model was constructed in mice, and osteoid formation was observed by hematoxylin-eosin (HE) staining. The interaction between lncRNA-ANCR and EZH2 was further identified by RNA pull-down assay. RESULTS: ANCR expression and intracellular calcium ions were increased in PMOP mice. Si-ANCR significantly increased the proliferation, ALP activity, calcium deposition of osteoblast cells and decreased apoptosis. ANCR and EZH2 were down-regulated by si-ANCR, while RUNX2 and OSTERIX were upregulated. Si-ANCR also promoted osteoid formation in mice treated with hydroxyapatite-tricalcium phosphate. In addition, ANCR specifically bound to EZH2. CONCLUSION: Silencing ANCR promotes the osteogenesis of PMOP osteoblast cells. The specific binding of ANCR with EZH2 suppressed RUNX2, thereby inhibiting osteogenesis.


Subject(s)
Alkaline Phosphatase , Animals , Apoptosis , Blotting, Western , Calcium , Female , Flow Cytometry , Humans , Ions , Mice , Microscopy, Confocal , Osteoblasts , Osteogenesis , Osteoporosis, Postmenopausal , Real-Time Polymerase Chain Reaction , RNA , RNA, Long Noncoding , RNA, Small Interfering , RNA, Untranslated , Transcription Factors , Transfection
10.
Article in English | WPRIM | ID: wpr-761909

ABSTRACT

BACKGROUND: Three-dimensional (3D) printed bone tissue engineering scaffolds have been widely used in research and clinical applications. β-TCP is a biomaterial commonly used in bone tissue engineering to treat bone defects, and its multifunctionality can be achieved by co-doping different metal ions. Magnesium doping in biomaterials has been shown to alter physicochemical properties of cells and enhance osteogenesis. METHODS: A series of Mg-doped TCP scaffolds were manufactured by using cryogenic 3D printing technology and sintering. The characteristics of the porous scaffolds, such as microstructure, chemical composition, mechanical properties, apparent porosity, etc., were examined. To further study the role of magnesium ions in simultaneously inducing osteogenesis and angiogenesis, human bone marrow mesenchymal stem cells (hBMSCs) and human umblical vein endothelial cells (HUVECs) were cultured in scaffold extracts to investigate cell proliferation, viability, and expression of osteogenic and angiogenic genes. RESULTS: The results showed that Mg-doped TCP scaffolds have the advantages of precise design, interconnected porous structure, and similar compressive strength to natural cancellous bone. hBMSCs and HUVECs exhibit high proliferation rate, cell morphology and viability in a certain amount of Mg²⁺. In addition, this concentration of magnesium can also increase the expression levels of osteogenic and angiogenic biomarkers. CONCLUSION: A certain concentration of magnesium ions plays an important role in new bone regeneration and reconstruction. It can be used as a simple and effective method to enhance the osteogenesis and angiogenesis of bioceramic scaffolds, and support the development of biomaterials and bone tissue engineering scaffolds.


Subject(s)
Biocompatible Materials , Biomarkers , Bone and Bones , Bone Marrow , Bone Regeneration , Cell Proliferation , Compressive Strength , Endothelial Cells , Humans , In Vitro Techniques , Ions , Magnesium , Mesenchymal Stem Cells , Methods , Osteogenesis , Porosity , Printing, Three-Dimensional , Veins
11.
Article in English | WPRIM | ID: wpr-761906

ABSTRACT

BACKGROUND: Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications. METHODS: In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (β-TCP) and strontium oxide (SrO). A composition of β-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/β-TCP (1:2 w/w), and COL/β-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization. RESULTS: All the scaffolds had a microporous structure with high porosity (~ 95–99%) and appropriate pore size (100–200 µm). COL/β-TCP-Sr scaffolds had the compressive modulus (213.44 ± 0.47 kPa) higher than that of COL/β-TCP (33.14 ± 1.77 kPa). In vitro cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of β-TCP-Sr to collagen scaffolds increased ALP activity by 1.33–1.79 and 2.92–4.57 folds after 7 and 14 days of culture, respectively. CONCLUSION: In summary, it was found that the incorporation of Sr into the collagen-β-TCP scaffolds has a great potential for bone tissue engineering applications.


Subject(s)
Alkaline Phosphatase , Animals , Biopolymers , Bone and Bones , Bone Marrow , Collagen , Fourier Analysis , Freeze Drying , In Vitro Techniques , Ions , Mesenchymal Stem Cells , Nanocomposites , Nanoparticles , Porosity , Rats , Spectrum Analysis , Strontium , X-Ray Diffraction
12.
Article in English | WPRIM | ID: wpr-761792

ABSTRACT

Confirming the direct link between neural circuit activity and animal behavior has been a principal aim of neuroscience. The genetically encoded calcium indicator (GECI), which binds to calcium ions and emits fluorescence visualizing intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. These GECI-based signals can be acquired by several tools including two-photon microscopy and microendoscopy for precise or wide imaging at cellular to synaptic levels. In addition, the images from GECI signals can be analyzed with open source codes including constrained non-negative matrix factorization for endoscopy data (CNMF_E) and miniscope 1-photon-based calcium imaging signal extraction pipeline (MIN1PIPE), and considering parameters of the imaged brain regions (e.g., diameter or shape of soma or the resolution of recorded images), the real-time activity of each cell can be acquired and linked with animal behaviors. As a result, GECI signal analysis can be a powerful tool for revealing the functions of neuronal circuits related to specific behaviors.


Subject(s)
Animals , Behavior, Animal , Brain , Calcium Channels , Calcium , Carisoprodol , Endoscopy , Fires , Fluorescence , Ions , Microscopy , Neuronal Calcium-Sensor Proteins , Neurons , Neurosciences , Statistics as Topic
13.
Article in English | WPRIM | ID: wpr-761782

ABSTRACT

Polycystic kidney disease 2-like-1 (PKD2L1), polycystin-L or transient receptor potential polycystin 3 (TRPP3) is a TRP superfamily member. It is a calcium-permeable non-selective cation channel that regulates intracellular calcium concentration and thereby calcium signaling. Although the calmodulin (CaM) inhibitor, calmidazolium, is an activator of the PKD2L1 channel, the activating mechanism remains unclear. The purpose of this study is to clarify whether CaM takes part in the regulation of the PKD2L1 channel, and if so, how. With patch clamp techniques, we observed the current amplitudes of PKD2L1 significantly reduced when coexpressed with CaM and CaMΔN. This result suggests that the N-lobe of CaM carries a more crucial role in regulating PKD2L1 and guides us into our next question on the different functions of two lobes of CaM. We also identified the predicted CaM binding site, and generated deletion and truncation mutants. The mutants showed significant reduction in currents losing PKD2L1 current-voltage curve, suggesting that the C-terminal region from 590 to 600 is crucial for maintaining the functionality of the PKD2L1 channel. With PKD2L1608Stop mutant showing increased current amplitudes, we further examined the functional importance of EF-hand domain. Along with co-expression of CaM, ΔEF-hand mutant also showed significant changes in current amplitudes and potentiation time. Our findings suggest that there is a constitutive inhibition of EF-hand and binding of CaM C-lobe on the channel in low calcium concentration. At higher calcium concentration, calcium ions occupy the N-lobe as well as the EF-hand domain, allowing the two to compete to bind to the channel.


Subject(s)
Binding Sites , Calcium , Calcium Signaling , Calmodulin , Ion Channels , Ions , Patch-Clamp Techniques , Polycystic Kidney Diseases , Transient Receptor Potential Channels
14.
Mycobiology ; : 217-229, 2019.
Article in English | WPRIM | ID: wpr-760537

ABSTRACT

Two manganese peroxidases (MnPs), MnP1 and MnP2, and a laccase, Lac1, were purified from Trametes polyzona KU-RNW027. Both MnPs showed high stability in organic solvents which triggered their activities. Metal ions activated both MnPs at certain concentrations. The two MnPs and Lac1, played important roles in dye degradation and pharmaceutical products deactivation in a redox mediator-free system. They completely degraded Remazol brilliant blue (25 mg/L) in 10–30 min and showed high degradation activities to Remazol navy blue and Remazol brilliant yellow, while Lac1 could remove 75% of Remazol red. These three purified enzymes effectively deactivated tetracycline, doxycycline, amoxicillin, and ciprofloxacin. Optimal reaction conditions were 50 °C and pH 4.5. The two MnPs were activated by organic solvents and metal ions, indicating the efficacy of using T. polyzona KU-RNW027 for bioremediation of aromatic compounds in environments polluted with organic solvents and metal ions with no need for redox mediator supplements.


Subject(s)
Amoxicillin , Biodegradation, Environmental , Ciprofloxacin , Doxycycline , Hydrogen-Ion Concentration , Ions , Laccase , Manganese , Oxidation-Reduction , Peroxidases , Pharmaceutical Preparations , Solvents , Tetracycline , Trametes
15.
Article in English | WPRIM | ID: wpr-763611

ABSTRACT

BACKGROUND: Medicines and Healthcare products Regulatory Agency (MHRA) guidance for patients with metal-on-metal (MoM) hip replacements was provided in 2012 and updated in 2017 to assist in the early detection of soft-tissue reactions due to metal wear debris. A large number of MoM hip replacements were undertaken at our hospital trust. A program of recall for all patients with MoM hip replacements was undertaken and MHRA guidelines were implemented. In this study, we aimed to investigate the effectiveness of the revised MHRA guidelines in the detection of early adverse reactions to metal debris and to re-evaluate the indications for metal artifact reduction sequence magnetic resonance imaging (MARS-MRI) and revision surgery. METHODS: Identification and recall of all patients with MoM hip replacements from 2001 were conducted by using theatre logs, patient records, clinical coding information, and consultant logbooks. Two senior arthroplasty consultants reviewed X-rays and patient records. Postal questionnaires were forwarded to patients, together with requests for general practitioners to complete cobalt and chromium blood tests. The two consultant-led review of MOM replacements was undertaken with further radiological investigations (X-rays, MARS-MRI) performed according to the 2017 guidance with support of consultant radiologists. RESULTS: Of 674 identified patients, 297 were available for review: 26 patients did not have MoM implants, 36 were untraceable, 59 refused follow-up, 87 moved out of area, 147 had died, and 22 already had revision. Of 297 patients, 126 were women and 171 were men; age range was 39 to 95 years (mean age, 69 years); 126 had resurfacing and 171 had MoM replacements. Twenty-six patients had elevated metal ions. Thirty-three patients underwent MARS-MRI: MARS-MRI results were positive in 17 and negative in 16. Of 17 patients with positive MARS-MRI, 10 patients were asymptomatic and seven were waiting revision. CONCLUSIONS: Positive MARS-MRI can often occur in the absence of elevated metal ion levels; elevated blood metal ion levels do not mean MARS-MRI will be positive. All patients with MoM replacements were at risk. It is imperative to assess patients regularly for symptoms that may raise clinical suspicion and maintain a low threshold to performing MARS-MRI.


Subject(s)
Arthroplasty , Artifacts , Chromium , Clinical Coding , Cobalt , Consultants , Delivery of Health Care , Female , Follow-Up Studies , General Practitioners , Hematologic Tests , Hip , Hospitals, District , Humans , Ions , Magnetic Resonance Imaging , Male
16.
Rev. odontol. Univ. Cid. São Paulo (Online) ; 30(1): 33-46, jan.-mar. 2018. tab.
Article in Portuguese | LILACS, BBO | ID: biblio-965197

ABSTRACT

The dolomite (DMT) can affect the metabolism of calcium and hydroxyl ions mineralization. To evaluate the toxicity, chemical properties and release of calcium and magnesium ions about 4 samples of DMT: Bioficina® - DMT I, Flora Pinhais® - DMT II, Dolomitex® - DMT III and Gran-White - DMT IV and hydroxide calcium PA (Biodinâmica® - HCA). Through bioassay Artemia Salina, hydrogen potential (pH), atomic absorption, X-ray diffraction (XRD) and X-ray fluorescence (XRF) of 4 samples of DMT was verified that the samples are suitable for potential use in dental materials. XRD and XRF techniques allowed to characterize the spatial conformation of the unit cell of dolomite, crystalline phases, mass percentage of chemical elements present in the samples. The presence of crystalline phases in addition to DMT has been identified as quartz and calcite. Impurities were detected in small amounts (Fe, K, Sr, Tm, S, Cu) and HCa to expectations about 100% portlandite. The pH was measured at concentrations of 1000 µg/mL;750 µg/mL;500 µg/mL;250 µg/ml and 100 ug/ml of the diluted crude extract of the sample at initial (0) and the periods of 24 and 168 hours wich were characterized in alkalinity pattern. In the interpretation of XRD and XRF tests have been detected the presence of silica, calcite and impurities besides the pure DMT in trace amounts in 2 samples while HCa to expectations of approximately 100% portlandite. To determine the toxicity was used the alternative method of lethal concentration 50 (CL50) with the bioassay model Artemia Salina. It resulted in low level of toxicity of DMTs with insignificant difference between times 24 and 48 hours. There was a release of 100ppm calcium and 32 ppm magnesium ions. None of the samples showed a significant percentage of other constituents considered harmful to health. It could be concluded that DMT is non-toxic, alkaline pH, considerable release of calcium ions, with crystalline phase that is characterized as a potential dental use.


A dolomita (DMT) afeta o metabolismo da mineralização por dissociação em íons cálcio e hidroxila. Avaliar a toxicidade, propriedades químicas e liberação de íons cálcio e magnésio de 4 amostras comerciais: Bioficina® - DMT I, Flora Pinhais® - DMT II, Dolomitex® - DMT III e Gran-White­DMT IV e do hidróxido de cálcio PA(Biodinâmica® - HCa). Com bioensaio Artemia Salina, potencial Hidrogeniônico(pH), absorção atômica, difração de raios X(DRX) e fluorescência de raios X(FRX) foi verificada a adequação do uso potencial para materiais odontológicos. DRX e FRX caracterizaram as fases cristalinas e percentual em massa de elementos químicos. Foi identificada a presença de fases cristalinas além da DMT, como quartzo e calcita. Impurezas foram detectadas e o HCa correspondeu às expectativas de aproximadamente 100% portlandita. O pH foi aferido em concentrações de 1000µg/mL;750µg/mL;500µg/mL;250 µg/mL e 100µg/mL do extrato bruto diluído das amostras nos tempos inicial (0) e 24 e 168horas. Sendo caracterizados no padrão de alcalinidade, DRX e FRX detectaram a presença de sílica, calcita e impurezas em quantidades mínimas em 2 das amostras e o HCa correspondeu às expectativas de aproximadamente 100% de portlandita. A toxicidade resultou em baixo índice com diferença insignificante entre os tempos 24 e 48h. Em média, ocorreu a liberação de íons: 100ppm de cálcio e 32ppm de magnésio. Nenhuma das amostras apresentou porcentagem significativa de constituintes considerados prejudiciais à saúde. Pôde-se concluir que a DMT é atóxica, pH alcalino, considerável liberação de íons cálcio, de fase cristalina que a caracteriza como potencial uso odontológico.


Subject(s)
Inorganic Particles , Chemical Properties , Toxicity , Tooth Calcification , Ions , Minerals
17.
An. acad. bras. ciênc ; 90(1): 541-555, Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-886922

ABSTRACT

ABSTRACT Vinasse is a byproduct of the process of distillation of sugarcane juice for the manufacture of sugar and alcohol. Because it is rich in nutrients, mainly potassium (K), is used as fertilizer and applied via fertigation, without concerning for the fate of this compound in the soil. Thus, the objective of the study was to evaluate the interactions of the potassium ion (K+), applied via vinasse in a soil representative of the sugarcane zone of the State of Pernambuco using adsorption isotherms. The methodology was based on physical, chemical and soil mineralogical characterization, as well as equilibrium batch tests, where the experimental curves were fitted by Langmuir and Freundlich isotherm models. The results allowed to infer that the Freundlich model showed better fit of the curve in both forms: linear and non-linear (direct fit); the non-linear model was selected due to the values ​​of coefficient of determination (R²). The interaction between potassium and soil occurred mainly with organic matter and the presence of soil kaolinite, because they showed negative ions on the external surface, thereby promoting potassium adsorption. Soil potassium adsorption capacity was higher for the first layer (0-20 cm) and decreased along the depth profile.


Subject(s)
Potassium/chemistry , Soil/chemistry , Reference Values , Linear Models , Adsorption , Saccharum/chemistry , Fertilizers , Ions , Models, Chemical
18.
Article in Chinese | WPRIM | ID: wpr-775401

ABSTRACT

The root bark of Dictamnus dasycarpus is one of common traditional Chinese medicines (TCMs). Quinoline alkaloids are one of the main active substances in this TCM and possess many biological activities including anti-titumor, anti-inflammation, anti-bacteria, anti-oxidation, and anti-platelet aggregation activities. In this study, eight quinoline alkaloids 1-8 were firstly separated from the root barks of D. dasycarpus. It was difficult to isolate more quinoline alkaloids from the remaining fraction 8 in D. dasycarpus by this conventional chemical separation, so the target analysis method combined LC-MS guided-separation of quinoline alkaloids from fraction 8 was established. MS/MS fragmentation patterns of eight quinoline alkaloids reference standard compounds 1-8 were studied by ultra-performance liquid chromatography-electrospary ionization-mass spectrometry (UPLC-ESI-MS/MS). Based on the feature fragment ion 200, the parent ion scan mode was established for the target analysis of quinoline alkaloids in fraction 8. Finally, 8-methoxyflindersine (9) and N-metilatanina (10) were discovered and isolated quickly from fraction 8 guided by LC-MS, and their structures were identified by NMR and MS. Among them, compound 10 was isolated from the genus Dictamnus for the first time. These results indicated that this method is not only quick and sensitive for analyzing the quinoline alkaloids, but also to effectively guided-separate this kind of alkaloids in the root barks of D. dasycarpus.


Subject(s)
Alkaloids , Chromatography, High Pressure Liquid , Dictamnus , Chemistry , Ions , Phytochemicals , Plant Roots , Chemistry , Quinolines , Spectrometry, Mass, Electrospray Ionization , Tandem Mass Spectrometry
19.
Article in English | WPRIM | ID: wpr-742277

ABSTRACT

The present study was performed to observe histopathological effects of Oculotrema hippopotami Stunkard, 1924 infection in the eye of Hippopotamus amphibius, as well as to reveal new details of morphology and structural features of this monogenean and its comparison between 2 age stages of the parasite. This was done using both light and scanning electron microscopy, energy dispersive X-ray analysis (EDXA) and histopathology. The presence of a mixture of different generations (adult and sub-adult) in one host individual is common for Oculotrema Stunkard, 1924 in contrast to Polystoma Zeder, 1800. New metrical and graphical information obtained for adults and sub-adults compared with the previous studies. Here we show the presence of genital papillae in adults, metrical data on the distal part of the vas deferens. SEM micrographs of sperm ejaculatory structures and information about the flattened dorsal side of the body provided for the first time. Histopathological changes, such as necrosis and hemorrhage in host tissues as a result of O. hippopotami attachment structures are described. Structural analysis of different body parts of O. hippopotami of both age groups are also included. We show qualitative differences in the presence of hardening ions (S, P, Ca) in attachment structures (oral and haptor suckers) that increase with the age of the worm. The presence of sub-adults and adults on the same host, together with high levels of infection without high pathogenicity may account for Oculotrema being one of the most successful parasites among the Monogenea.


Subject(s)
Adult , Family Characteristics , Hemorrhage , Human Body , Humans , Ions , Microscopy, Electron, Scanning , Necrosis , Parasites , Spermatozoa , Vas Deferens , Virulence
20.
Annals of Dermatology ; : 265-275, 2018.
Article in English | WPRIM | ID: wpr-715500

ABSTRACT

Epidermal barrier formation and the maintenance of barrier homeostasis are essential to protect us from the external environments and organisms. Moreover, impaired keratinocytes differentiation and dysfunctional skin barrier can be the primary causes or aggravating factors for many inflammatory skin diseases including atopic dermatitis and psoriasis. Therefore, understanding the regulation mechanisms of keratinocytes differentiation and skin barrier homeostasis is important to understand many skin diseases and establish an effective treatment strategy. Calcium ions (Ca²⁺) and their concentration gradient in the epidermis are essential in regulating many skin functions, including keratinocyte differentiation, skin barrier formation, and permeability barrier homeostasis. Recent studies have suggested that the intracellular Ca²⁺ stores such as the endoplasmic reticulum (ER) are the major components that form the epidermal calcium gradient and the ER calcium homeostasis is crucial for regulating keratinocytes differentiation, intercellular junction formation, antimicrobial barrier, and permeability barrier homeostasis. Thus, both Ca²⁺ release from intracellular stores, such as the ER and Ca²⁺ influx mechanisms are important in skin barrier. In addition, growing evidences identified the functional existence and the role of many types of calcium channels which mediate calcium flux in keratinocytes. In this review, the origin of epidermal calcium gradient and their role in the formation and regulation of skin barrier are focused. We also focus on the role of ER calcium homeostasis in skin barrier. Furthermore, the distribution and role of epidermal calcium channels, including transient receptor potential channels, store-operated calcium entry channel Orai1, and voltage-gated calcium channels in skin barrier are discussed.


Subject(s)
Calcium Channels , Calcium , Dermatitis, Atopic , Endoplasmic Reticulum , Epidermis , Homeostasis , Intercellular Junctions , Ions , Keratinocytes , Permeability , Psoriasis , Skin Diseases , Skin , Transient Receptor Potential Channels
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