Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 63
Filter
1.
Arq. ciências saúde UNIPAR ; 26(1): 1-12, Jan-Abr. 2022.
Article in Portuguese | LILACS | ID: biblio-1362657

ABSTRACT

Mesmo com a evolução do conhecimento científico, a utilização de plantas medicinais é uma das práticas mais antigas ainda realizadas pelo homem, especialmente pelos habitantes de comunidades e municípios do interior do Brasil, principalmente em regiões com baixos índices de desenvolvimento humano. O objetivo deste trabalho foi realizar um estudo etnobotânico sobre as plantas medicinais utilizadas pelos moradores de um bairro localizado em um município ribeirinho do interior do estado do Amazonas. Participaram do estudo cem indivíduos, e os dados obtidos pela pesquisa foram compilados por meio de um formulário, contendo 14 questões (objetivas e discursivas). Os resultados demonstraram que 95% dos moradores faziam uso de plantas medicinais. Foram citadas 89 espécies, pertencentes a 48 famílias botânicas, com destaque para a Lamiaceae. As plantas mais citadas foram: Plectranthus barbatus (boldo) ­ 6,1%, Citrus sinensis (laranjeira) ­ 5,8% e Allium sativum (alho) ­ 4,7%. No entanto, quando analisado o índice de concordância (porcentagem de uso principal = CUP), a Citrus sinensis (laranjeira) apresentou um CUP de 80,9%, seguida por Plectranthus barbatus (boldo) e Disphania ambrosioides (mastruz), com 68,1% e 61,5%, respectivamente. Com relação ao modo de preparo, 61,1% citaram infusão, e as principais enfermidades tratadas foram as dores de estômago, com 13,1%. É importante enfatizar a necessidade de mais estudos acerca dos reais benefícios dessas ervas, a fim de que a divulgação de tais informações possa complementar o conhecimento empírico já difundido entre a população local. Dessa forma, será possível ampliar o conhecimento etnobotânico como um todo e, consequentemente, promover saúde e bem-estar.


Despite the evolution of scientific knowledge, the use of medicinal plants is one of the oldest practices still used by men and, especially by the inhabitants of communities and municipalities of the interior of Brazil, mainly in regions with low human development rates. The purpose of this work was to perform an ethnobotanical study on medicinal plants used by residents of a neighborhood located in a riverside city in the interior of the state of Amazonas. A total of 100 individuals participated in the study, with data being obtained through the use of a form containing 14 open and closed-ended questions. The results showed that 95% of the residents used medicinal plants. A total of 89 species, belonging to 48 botanical families were mentioned, with emphasis to Lamiaceae. The plants that were most frequently mentioned were Plectranthus barbatus (locally known as boldo) ­ 6.1%; Citrus sinensis (orange tree) ­ 5.8%; and Allium sativum (garlic) ­ 4.7%. However, when analyzing the agreement index (percentage of main use = CUP), Citrus sinensis (orange tree) presented a CUP of 80.9%, followed by Plectranthus barbatus (boldo) and Disphania ambrosioides (Mexican tea), with 68.1 and 61.5%, respectively. In relation to the method of preparation, 61.1% cited infusion, with the main disorder treated being stomachache, with 13.1%. It is important to emphasize the need for further studies on the actual benefits of these herbs so that the dissemination of the information can complement the already widespread empirical knowledge among the local population. Thus, it will be possible to expand the ethnobotanical knowledge as a whole and, consequently, promote health and well-being.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Plants, Medicinal/adverse effects , Ethnobotany , Tea/adverse effects , Agricultural Cultivation , Lamiaceae/adverse effects , Peumus/adverse effects , Citrus sinensis/adverse effects , Diarrhea/prevention & control , Influenza, Human/therapy , Juices , Inflammation/prevention & control , Phytotherapy
2.
Rev. ADM ; 78(2): 73-79, mar.-abr. 2021. ilus, tab
Article in Spanish | LILACS | ID: biblio-1247336

ABSTRACT

El color es un efecto visual de los rayos de luz reflejándose y su concepto es complejo por ser una sensación que se percibe y por las características electromagnéticas. Los dientes varían espacialmente porque son curvados, tienen prolongaciones relativamente pequeñas y vistas en contra de una variable de fondo no uniforme así como típicamente una iluminación no estandarizada, por lo cual difieren en relación con su colorimetría, por lo que el estudio del color es fundamental en la odontología. En la actualidad hay métodos para evaluar el color, desde una simple revisión visual hasta instrumentos como el colorímetro y los espectrofotómetros, los cuales son aparatos utilizados en la medida del color de un objeto a través de su longitud de onda reflejada. Una pigmentación dental se produce por varios factores, ya sean intrínsecos y extrínsecos, estas pigmentaciones son factores importantes tanto en la estética como en el aspecto físico, por lo que es importante poder evaluar la estabilidad de los dientes naturales ante diferentes sustancias que podrían modificar su color natural. En este estudio nos dimos a la tarea de evaluar el cambio de color de dientes naturales ante diferentes bebidas, se eligieron tres bebidas pigmentantes y de uso común: café, vino tinto y jugo de arándano; se utilizaron 10 dientes unirradiculares del mismo color previamente analizados con el espectrofotómetro. Un diente fue la muestra control y los nueve restantes se sumergieron en frascos separados con 10 mL de las tres bebidas elegidas. Realizando la evaluación de color a los 15, 30 y 90 días con ayuda del espectrofotómetro, pudimos observar que el diente sumergido en café no tuvo variación durante los primeros 15 días y el cambio más notable de color fue hasta los 90 días a diferencia de las muestras sumergidas en vino y jugo de arándano cuya variación máxima de color se presentó en 15 días respectivamente (AU)


Color is a visual effect of light rays reflecting and its concept is complex, for being a sensation that is perceived and for the electromagnetic characteristics. Teeth vary spatially because they are curved, have relatively small extensions, and are viewed against a non-uniform background variable as well as typically non-standardized illumination, which is why they differ in relation to their colorimetry. So the study of color is fundamental in dentistry. Currently, there are methods to evaluate color, from a simple visual check to instruments such as the colorimeter and spectrophotometers, which are devices used to measure the color of an object through its reflected wavelength. A dental pigmentation is produced by various factors, both intrinsic and extrinsic, these pigmentations are currently important factors in both aesthetics and physical appearance, so it is important to be able to evaluate the stability of natural teeth against different substances that could modify its natural color. In this study, we undertook the task of evaluating the change in the color of natural teeth when faced with different beverages. Three pigment and commonly used beverages were chosen: coffee, red wine and cranberry juice; 10 single-rooted teeth of the same color previously analyzed with the spectrophotometer were used. One tooth was the control sample and the remaining nine were immersed in separate bottles with 10 mL of the three chosen drinks. Carrying out the color evaluation at 15, 30 and 90 days with the help of the spectrophotometer, we could see that the tooth immersed in coffee did not change during the first 15 days and the most notable change in color was up to 90 days, unlike the samples immersed in wine and cranberry juice whose maximum color variation was presented in 15 days respectively (AU)


Subject(s)
Humans , Tooth Discoloration , Beverages , Color , Colorimetry , Esthetics, Dental , Spectrophotometry/methods , Wine , In Vitro Techniques , Epidemiology, Descriptive , Statistical Analysis , Coffee , Juices , Light
3.
Actual. osteol ; 17(2): 69-77, 2021. tab
Article in Spanish | LILACS, BINACIS, UNISALUD | ID: biblio-1370075

ABSTRACT

El calcio (Ca) es un nutriente crítico para la salud, especialmente en los períodos de crecimiento. Sin embargo, la mayoría de los individuos argentinos no alcanzan la ingesta diaria recomendada. Por su parte, el fósforo (P) es un nutriente cuya ingesta suele estar por encima de la recomendada. Este tipo de desequilibrio en la ingesta de ambos nutrientes conlleva a una pérdida de masa ósea. El consumo de bebidas analcohólicas (BA) se ha incrementado en los últimos años, sobre todo en la población infantil, desplazando el consumo de agua y lácteos. El objetivo de este trabajo fue estimar el aporte de Ca y P a partir de BA diferentes de agua. Se evaluaron 59 muestras, cuya mediana y rango de Ca fue de 7,74 [0,00 a 111,29] mg/l y de P 55,17 [0,16 a 957,00] mg/l. Los jugos en polvo son los que mayor contenido de Ca presentaron y las bebidas deportivas aquellas donde se halló el mayor contenido de P. Considerando un consumo de 500 ml/día de BA se estarían incorporando 3,87 mg Ca y 27,59 mg P. El creciente consumo de BA, su bajo contenido de Ca y la concomitante reducción del consumo de lácteos contribuyen a una inadecuada ingesta de Ca. (AU)


Calcium (Ca) is a critical nutrient, especially during periods of growth. However, the majority of Argentine individuals do not reach the recommended daily intake. On the other hand, phosphorus (P) is a nutrient with an intake usually above the recommended values. This type of imbalance between the intake of the nutrients leads to loss of bone mass. Soft drinks consumption (BA) has increased in recent years, especially in children, displacing the consumption of water and dairy products. The aim of this work was to estimate the Ca and P content in BA other than water. 59 samples were evaluated, with a median and range of Ca of 7.74 [0.00 to 111.29] mg/l and of P of 55.17 [0.16 to 957.00] mg/l. Powdered juices are the ones with the highest Ca content, and sports drinks are the beverages in which the highest P content was found. Based on a BA consumption of 500 ml/day, 3.87 mg Ca and 27.59 mg P would be incorporated. Therefore, the increased consumption of BA, their low Ca content, and the concomitant reduction in dairy consumption contribute to an inadequate intake of Ca. (AU)


Subject(s)
Humans , Child , Adolescent , Phosphates/analysis , Carbonated Beverages/statistics & numerical data , Calcium/analysis , Juices , Argentina , Quality Control , Calcium, Dietary , Atomic Absorption Spectroscopy , Statistical Analysis , Growth and Development , Recommended Dietary Allowances
4.
Electron. j. biotechnol ; 46: 55-64, jul. 2020. tab, graf
Article in English | LILACS | ID: biblio-1223246

ABSTRACT

BACKGROUND: Ethanol concentration (PE), ethanol productivity (QP) and sugar consumption (SC) are important values in industrial ethanol production. In this study, initial sugar and nitrogen (urea) concentrations in sweet sorghum stem juice (SSJ) were optimized for high PE (≥10%, v/v), QP, (≥2.5 g/L·h) and SC (≥90%) by Saccharomyces cerevisiae SSJKKU01. Then, repeated-batch fermentations under normal gravity (NG) and high gravity (HG) conditions were studied. RESULTS: The initial sugar at 208 g/L and urea at 2.75 g/L were the optimum values to meet the criteria. At the initial yeast cell concentration of ~1 × 108 cells/mL, the PE, QP and SC were 97.06 g/L, 3.24 g/L·h and 95.43%, respectively. Repeated-batch fermentations showed that the ethanol production efficiency of eight successive cycles with and without aeration were not significantly different when the initial sugar of cycles 2 to 8 was under NG conditions (~140 g/L). Positive effects of aeration were observed when the initial sugar from cycle 2 was under HG conditions (180­200 g/L). The PE and QP under no aeration were consecutively lower from cycle 1 to cycle 6. Additionally, aeration affected ergosterol formation in yeast cell membrane at high ethanol concentrations, whereas trehalose content under all conditions was not different. CONCLUSION: Initial sugar, sufficient nitrogen and appropriated aeration are necessary for promoting yeast growth and ethanol fermentation. The SSJ was successfully used as an ethanol production medium for a high level of ethanol production. Aeration was not essential for repeated-batch fermentation under NG conditions, but it was beneficial under HG conditions.


Subject(s)
Saccharomyces cerevisiae/metabolism , Sorghum/chemistry , Ethanol/metabolism , Saccharomyces cerevisiae/growth & development , Urea , Yeasts/growth & development , Aeration , Sorghum/microbiology , Ethanol/analysis , Sugars , Juices , Fermentation , Gravitation , Nitrogen
5.
São Paulo; s.n; s.n; 2020. 88 p. ilus, graf, tab.
Thesis in English | LILACS | ID: biblio-1291873

ABSTRACT

Obesity is a worldwide public health problem and the main risk factor for a number of chronic diseases. Breast cancer is another worrisome disease: it is the leading cause of cancer amongst women and has an elevated mortality rate. Approximately 30 years ago, Barker and Trichopoulos suggested that cardiovascular disease and breast cancer, respectively, may be originated in utero. In subsequent years, studies proved both hypotheses correct. Understanding how in-utero environment can affect development of obesity and breast cancer in adulthood is key for preventing these diseases. Nutrition during gestation and lactation is considered a modifiable factor to impact in-utero environment. Orange juice (OJ) is an excellent source of bioactive compounds, including vitamin C and flavonoids, and reports suggests that intake of orange juice minimizes damaging effects of obesity. The objective of this thesis was to evaluate the effects of maternal obesity during gestation and lactation and OJ intake on (a) metabolic profile of male offspring exposed to control and obesogenic diets and (b) breast cancer risk of female offspring. Four-week-old C57BL/6 female mice were assigned into three groups: control- fed a control diet and water ad libitum, obese- fed obesogenic diet and water ad libitum and obese+OJ- fed obesogenic diet and OJ. After three weeks on the diet, females were mated to control males. Male offspring from each group were weaned into control or obesogenic diets for 21 weeks. Female offspring was either euthanized for evaluation of mammary gland development or submitted to a chemically induced breast carcinogenesis protocol. Parameters to assess metabolism (as body composition and adipose tissue expression of obesity-related genes), breast cancer risk (as epithelial elongation and number of terminal end buds) and tumorigenesis (incidence, latency and multiplicity of mammary tumors) were collected. ANOVA followed by Tukey or Fischer´s LSD test were used to investigate differences between groups and a p<0.05 was considered significant. Male offspring of obese mothers fed control diet presented increased glucose concentrations and expression of F4/80 and interleukin-6 compared to control offspring. Male offspring of obese+OJ mothers fed a control diet presented expression of F4/80 and interleukin-6 similar to control offspring. Male offspring to control mothers fed obesogenic diet presented increased glucose concentrations and epidydimal fat tissue compared to offspring of obese mothers. Offspring to obese+OJ mothers presented increased expression of leptin and tumor necrosis factor-α. Female offspring of obese mothers had decreased terminal end buds and increased latency of first tumor and OJ intake decreased epithelial elongation compared to offspring of control/obese mothers. Maternal obesity had greater impact in offspring exposed to control than obesogenic environment. OJ intake by mothers helped with harmful effects induced by maternal obesity on male offspring fed control diet. Control offspring exposed to obesogenic diet presented worse metabolic profile than offspring from obese mothers. In this particular case, OJ was not beneficial to male offspring. Whereas obesity induced by a high-fat high-sugar diet presented a somewhat protective effect on breast cancer risk, OJ further protected offspring of obese mothers


A obesidade é um problema de saúde pública e o principal fator de risco para uma série de doenças crônicas. O câncer de mama é outra doença preocupante: é a principal causa de câncer entre as mulheres e tem elevada taxa de mortalidade. Há 30 anos, Barker e Trichopoulos sugeriram que a doença cardiovascular e o câncer de mama, respectivamente, podem ser originados no útero. Nos anos seguintes essas hipóteses foram confirmadas. Compreender como o ambiente intrauterino pode afetar o desenvolvimento da obesidade e o câncer de mama na idade adulta, portanto, é fundamental para prevenir essas doenças. O estado nutricional e a nutrição durante a gestação e lactação são considerados fatores modificáveis e que pode influenciar o ambiente intrauterino. O suco de laranja (SL) é uma excelente fonte de compostos bioativos, incluindo vitamina C e flavonoides, e estudos sugerem que a ingestão de suco de laranja pode minimizar os efeitos deletérios da obesidade. O objetivo dessa tese foi de avaliar os efeitos da obesidade materna durante a gestação e lactação e a ingestão de SL no (a) perfil metabólico da prole masculinas expostos à dietas controle e obesogênica e (b) risco de câncer de mama da prole feminina. Camundongos fêmeas C57BL/6, com quatro semanas de idade, foram distribuídos em três grupos: controle - alimentados com uma dieta de controle e água ad libitum, obesosalimentados com dieta obesogênica e água ad libitum e obesos+SL- alimentados com dieta obesogênica e SL. Após três semanas na dieta, as fêmeas foram acasaladas com machos controle. A prole masculina de cada grupo foi desmamada e alimentadas com dieta obesogênica ou controle por 21 semanas. A prole feminina foi eutanasiada para a avaliação do desenvolvimento da glândula mamária ou submetida a um protocolo de carcinogênese mamária quimicamente induzida. Parâmetros para avaliar o metabolismo (como a composição corporal e expressão de genes relacionados à obesidade do tecido adiposo), risco de câncer de mama (como desenvolvimento epitelial e número de terminal end buds) e tumorigênese (incidência, latência e multiplicidade dos tumores mamários) foram coletados. Para investigar diferença estatística entre os grupos foi realizada ANOVA, seguida pelo teste de Tukey ou LSD de Fischer e um p<0,05 foi considerado significante. A prole masculina de mães obesas alimentadas com dieta de controle apresentou aumento das concentrações de glicose e aumento das expressões de F4/80 e interleucina-6 em relação a prole controle. A prole masculina de mães obesas+SL alimentadas com dieta controle apresentou expressão de F4/80 e interleucina-6 similar à da prole de controle. A prole masculina de mães controle e alimentada com dieta obesogênica apresentou aumento das concentrações de glicose e aumento do tecido adiposo epididimal em comparação à prole de mães obesas. A prole de mães obesas+SL apresentou maior expressão de leptina e TNF-α. A prole feminina de mães obesas apresentou redução do número de terminal end buds e aumento da latência para o aparecimento do primeiro tumor. O consumo de SL diminuiu o desenvolvimento epitelial comparado as proles de mães controles e obesas. A obesidade materna teve maior impacto na prole masculina exposta a dieta controle do que na obesogênica. A ingestão materna de SL ajudou com efeitos danosos induzidos pela obesidade materna na prole masculina alimentada com dieta controle. A prole de fêmeas controles e alimentada com dieta obesogênica apresentou perfil metabólico pior que a prole das mães obesas. Neste caso, o SL não foi benéfico para a prole masculina. A obesidade materna induzida por uma dieta rica em banha e açúcares apresentou discreto efeito protetor no risco de câncer de mama, o SL acentuou esta proteção


Subject(s)
Breast Neoplasms/pathology , Lactation , Pregnancy , Juices , Obesity, Maternal/classification , Metabolism , Obesity/classification , Women , Risk , Citrus sinensis
6.
Con-ciencia (La Paz) ; 8(2): 61-74, 2020. ilus., tab.
Article in Spanish | LILACS, LIBOCS | ID: biblio-1147992

ABSTRACT

INTRODUCCIÓN: la validación es el establecimiento de la evidencia documental de que un procedimiento analítico conducirá, con un alto grado de seguridad a la obtención de resultados precisos y exactos dentro de las especificaciones y los atributos de calidad previamente establecidos. OBJETIVO: Validar el método analítico por cromatografía líquida de alta resolución para la cuantificación de conservantes en jugo de naranja. MÉTODOS: el método fue validado siguiendo los parámetros referidos en la USP 40 ­ NF 35: especificidad, linealidad, precisión (repetibilidad instrumental, repetibilidad del método y precisión intermedia) y exactitud. RESULTADOS: el método analítico propuesto es selectivo/específico, presenta una excelente linealidad dentro del rango de 100 a 1000 ppm, la Precisión presentó resultados de CV < 2% siendo óptimos. La recuperación se encuentra entre 97 a 102% demostrando excelente exactitud. CONCLUSIÓN: se cumplieron todos los parámetros y criterios de validación por lo tanto el método es específico, selectivo, lineal en el intervalo de concentraciones de 100 a 1000 ppm, sensible, preciso, reproducible y exacto


INTRODUCTION: validation is the establishment of documentary evidence that an analytical procedure will lead, with a high degree of security, to obtaining precise and exact results within the specifications and previously established quality attributes. OBJECTIVE: validate the analytical method by high resolution liquid chromatography for the quantification of preservatives in orange juice. METHODS: the method was validated following the parameters referred to in USP 40 - NF 35, they are specificity, linearity, precision (instrumental repeatability, repeatability of the method and intermediate precision) and accuracy. RESULTS: the proposed analytical method is selective/specific, it presents an excellent linearity within the range of 100 to 1000 ppm, the precision presented results of CV <2% being optimal. The recovery is between 97 to 102% CONCLUSION: all the parameters and validation criteria were met, therefore the method is specific, selective, linear in the concentration range of 100 to 1000 ppm, sensitive, precise, reproducible and exact


Subject(s)
Chromatography, Liquid , Quality Management , Citrus sinensis , Sensitivity and Specificity , Juices , Methods
7.
Braz. arch. biol. technol ; 63: e20180413, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132167

ABSTRACT

Abstract: Freshly prepared apple tea wine (a combination of tea extract and apple juice) is having yeasty and dull flavour, which needs to be improved to increase the acceptability of this product. Therefore, an attempt has been made for artificial ageing of apple tea wine using different wood chips to improve its physico-chemical, sensory and antimicrobial attributes. Different types of wood chips (Quercus spp., Bombax spp. and Acacia spp.) were added respectively (2.5 g/L to the freshly prepared apple tea wine) and allowed for ageing in carboys for the six months at the room temperature. The influence of each wood species on physico-chemical, sensory and antimicrobial attributes was tested upto 6 months of storage. Storage intervals significantly affected all the physico-chemical attributes (except total sugars, volatile acidity, and antioxidant activity), whereas, the addition of wood chips affected titratable acidity, ethanol, higher alcohols, total phenols, and amino acid. Cluster analysis of the physico-chemical attributes data revealed the same and showed that storage intervals exerted more effect on the physico-chemical and antimicrobial properties of the apple tea wine rather than the wood chips. The antimicrobial activity of 6 months aged wine was low as compared to the fresh wine. Among all the wood chips, apple tea wine aged with Quercus spp. possesses a significantly higher score (according to desirability) than the wine aged with other wood chips and control. In nutshell, apple tea wine matured with Quercus spp. wood chips for 6 months were the best with improved physico-chemical and sensory attributes.


Subject(s)
Humans , Tea/chemistry , Wine/analysis , Malus/chemistry , Juices , Anti-Bacterial Agents/pharmacology , Chemical Phenomena
8.
Electron. j. biotechnol ; 41: 13-21, sept. 2019. ilus, graf, tab
Article in English | LILACS | ID: biblio-1053560

ABSTRACT

Background: Sugars from sweet sorghum stalks can be used to produce ethanol and also to grow oleaginous yeasts. Instead of two separate processes, in this paper we propose a different route producing ethanol and microbial oil in two consecutive fermentation steps. Results: Three yeasts were compared in the first ethanol producing step. In the second step four different oleaginous yeasts were tested. Sweet sorghum juice was first clarified and concentrated. High gravity ethanol fermentation was carried out with concentrated juice with 23.7 g/100 mL of total sugars and without added nutrients. Total sugars were 2.5 times more than the original clarified juice. One yeast gave the best overall response over the two other tested; relative high ethanol productivity, 1.44 g ethanol/L•h−1 , and 90% of sugar consumption. Aeration by flask agitation produced superior results than static flasks for all yeasts. Microbial oil production was done employing the residual liquid left after ethanol separation. The pooled residual liquid from the ethanol distillation contained 7.08 g/mL of total carbohydrates, rich in reducing sugars. Trichosporon oleaginosus and Lipomyces starkeyi produced higher dry biomass, total sugar consumption and oil productivity than the other two oleaginous yeasts tested; with values around 25 g/L, 80%, and 0.55 g oil/L•h−1 respectively. However, the biomass oil content in all yeasts was relatively low in the range of 14 to 16%. Conclusion: The two step process is viable and could be considered an integral part of a consolidated biorefinery from sweet sorghum.


Subject(s)
Sorghum/chemistry , Ethanol/metabolism , Fruit and Vegetable Juices , Saccharomyces cerevisiae , Yeasts , Trichosporon , Sugars , Juices , Lipomyces , Biofuels/microbiology , Fermentation
9.
Hig. aliment ; 32(280/281): 74-78, 30/06/2018. ilus
Article in Portuguese | LILACS | ID: biblio-910580

ABSTRACT

Lactobacillus rhamnosus GG é uma bactéria probiótica, Gram-positiva, não esporulada, catalase negativa, microaerofílica e não fermentadora de lactose. Assim, produtos lácteos fermentados não são bons carreadores desse micro-organismo, mas, produtos de origem vegetal, como sucos, podem veicular essa bactéria. Entretanto, a acidez desses alimentos pode inibir a viabilidade de probióticos. Por outro lado, a exposição de micro-organismos a um estresse prévio subletal faz com que ocorra a ativação de mecanismos celulares regulatórios e os mesmos quando expostos a uma condição de estresse subletal previamente são mais resistentes às condições de estresse subsequentes. Neste trabalho avaliou-se o estresse subletal pelo frio em células de L. rhamnosus GG em suco misto de juçara e manga durante 60 dias de armazenamento a 7 °C. Foram realizados dois tratamentos sendo eles, controle e estresse subletal pelo frio no qual L. rhamnosus GG foi armazenado a 4 °C por sete dias em caldo deMan Rogosa Sharpe (MRS). Após, a centrifugação foi realizada e o pellet de células foi inoculado em suco misto de juçara e manga. A análise de viabilidade por plaqueamento foi realizada nos tempos 0, 7, 14, 21, 28, 45 e 60 dias em ágar MRS. Com 28 dias de armazenamento houve redução de 0,59 ciclo logaritimico na contagem para o tratamento controle e 0,80 ciclo logaritimico para o tratamento em que as células passaram pelo estresse subletal pelo frio e com 60 dias houve redução da contagem do tratamento controle de 1,69 ciclo logaritimico e para o tratamento de estresse subletal de 1,9 ciclo logaritimico. Dessa forma, o estresse subletal pelo frio aplicado não foi suficiente para ativar os mecanismos de resposta ao estresse em L. rhamnosus GG quando veiculado em suco misto de juçara e manga.


Lactobacillus rhamnosus GG is a probiotic, Gram-positive, non-spore forming, catalase-negative, microaerophilic and non-fermenting lactose bacterium. Therefore, fermented dairy products are not good carriers of this microorganism, so products of vegetable origin, such as juices, can vehicle this probiotic. However, the acidity present can inhibit the viability of probiotic microorganisms. On the other hand, the exposure of microorganisms to a previous sublethal stress causes the activation of regulatory cellular mechanisms. Therefore, microorganisms that are exposed to a sublethal stress condition previously are more resistant. This work evaluated the sublethal cold stress in L. rhamnosus GG cells in mixed juçara and mango juice for 60 days at 7 °C. Two treatments were carried out, being control and sublethal cold stress in which L. rhamnosus GG was stored at 4 °C for seven days in Man Rogosa Sharpe broth (MRS). After centrifugation, the cell pellet was inoculated in mixed juçara and mango juice. The viability analysis by plating was performed at times 0, 7, 14, 21, 28, 45 and 60 days. With 28 days of storage there was a reduction of 0.59 logarithmic cycle for the control and 0.80 logarithmic cycle for the treatment of sublethal stress, and with 60 days the count of L. rhamnosus GG in the control treatment reduced 1.69 logarithmic cycle and 1.9 logarithmic cycle for the treatment of sublethal stress. Thus, the application of sublethal stress by cold was not enough to activate the mechanisms of stress response in L. rhamnosus GG when served by mixed juçara and mango juice.


Subject(s)
Probiotics , Mangifera , Functional Food , Juices , Food Storage , Euterpe , Bacteria , Lactobacillus rhamnosus
10.
Hig. aliment ; 32(278/279): 74-78, 30/04/2018.
Article in Portuguese | LILACS | ID: biblio-909979

ABSTRACT

Este trabalho teve por objetivo avaliar as características higienicossanitárias dos refrescos armazenados em garrafas tipo PET e em refresqueiras comercializados em estabelecimentos de um município do Recôncavo da Bahia. Foram analisadas, quanto à presença de bolores, leveduras e coliformes termotolerantes, 10 amostras de refrescos, acondicionadas em garrafas PET e refresqueiras. Das cinco amostras analisadas, duas (40%) armazenadas em garrafas PET, e uma (20%) armazenada em refresqueiras, apresentou-se em desacordo com os padrões estabelecidos pela legislação vigente. Na análise de bolores e leveduras, encontrou-se elevado crescimento destes micro- -organismos em quatro amostras (80%) armazenadas em garrafas PET e em cinco amostras (100%) armazenadas em refresqueiras. As informações obtidas nessa pesquisa demonstraram que os refrescos coletados estavam em condições higienicossanitárias insatisfatórias, podendo representar causas de infecções de origem alimentar aos consumidores.(AU)


The objective of this study was to evaluate the hygienic-sanitary characteristics of refreshment stored in PET bottles and in refreshments marketed in establishments in a municipality in the Recôncavo da Bahia. For the presence of molds, yeasts and thermotolerant coliforms, 10 samples of refreshment, packed in PET bottles and coolers, were analyzed. Of the five samples analyzed, two (40%) stored in PET bottles, and one (20%) stored in refrigerators, were in disagreement with the standards established by current legislation. When analyzing molds and yeasts, a high growth of these microorganisms was found in four samples (80%) stored in PET bottles and in five samples (100%) stored in coolants. The information obtained in this research showed that the refreshments collected were in unsatisfactory hygienic-sanitary conditions, and may represent causes of food-borne infections to consumers.


Subject(s)
Humans , Food Contamination/analysis , Juices , Food Microbiology , Yeasts/isolation & purification , Brazil , Food Packaging , Food Storage/standards , Coliforms , Multiple Tube Method/methods , Fungi/isolation & purification , Legislation, Food
11.
Pesqui. bras. odontopediatria clín. integr ; 18(1): 3888, 15/01/2018. tab, graf
Article in English | LILACS, BBO | ID: biblio-965759

ABSTRACT

Objective: To determine the effect of seven different beverages on tooth enamel. Material and Methods: Human teeth samples were immersed in each beverage (Pepsi, Red Bull, Orange juice, Apple juice, Lemon juice, Coffee and Green tea). Specimens were divided into seven groups by beverage type (n = 5 per group). Weight of the enamel was measured before and after immersion in different beverages daily for at periodic intervals of 24hrs, 7 days, 15 days and 30 days. The data were recorded after weighing the teeth at each time. The beverages were replaced daily. Anova and Tukey post hoc test was performed to compare the differences in percent weight loss. The significance level was set at 5%. Results: There was change in the mean percent weight loss amongst the different beverages with orange juice showing a high reduction in comparison to green tea and coffee. The mean weight reduction of orange juice was by 21% followed by Red Bull (13%), Pepsi (11%), Lemon juice (16%), Apple juice (16%), coffee (3%) and green tea (3%). Conclusion: This study shows that different beverages have erosive potential on teeth depending on the duration of the exposure.


Subject(s)
Humans , Tooth Erosion/diagnosis , Beverages/analysis , Dental Enamel , Juices , Fruit , Analysis of Variance
12.
Hig. aliment ; 31(270/271): 55-59, 29/08/2017.
Article in Portuguese | LILACS | ID: biblio-848791

ABSTRACT

Este trabalho teve como objetivo verificar a qualidade microbiológica do suco de laranja in natura por meio de contagem de coliformes totais e termotolerantes e correlacionar os resultados obtidos com as condições de higiene do estabelecimento produtor através da aplicação de um checklist. Foram coletadas quatro amostras de suco de laranja in natura preparadas na hora. As amostras foram analisadas quanto ao Número Mais Provável (NMP) de coliformes totais e termotolerantes pela metodologia de tubos múltiplos de fermentação. O pH 4 foi identificado em todas as amostras. Foram observadas inadequações nos checklist e observou-se também que 50% das amostras analisadas apresentaram contagem de coliformes totais e termotolerantes acima do permitido pela Legislação. Conclui-se que são necessários treinamentos periódicos para os funcionários e proprietário do estabelecimento para que as condições higienicossanitárias sejam adequadas à produção de alimentos sem oferecer riscos à saúde do consumidor.(AU)


Subject(s)
Humans , Citrus sinensis/microbiology , Coliforms , Food Contamination/analysis , Food Microbiology , Juices , Checklist/statistics & numerical data , Cooking and Eating Utensils , Food Handling , Food Inspection , Foods Equipment
13.
Rev. Bras. Saúde Mater. Infant. (Online) ; 17(3): 491-500, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-1013042

ABSTRACT

Abstract Objectives: to describe the consumption of sugar sweetened beverages in Colombia, South America and variables associated with this consumption. Methods: based on the Food Frequency Questionnaire applied in the National Survey of the Nutritional Situation of Colombia (2010), the prevalence and frequency/day of the consumption of sugar sweetened beverages by 10,070 subjects between 5 and 17 years old was estimated. Results: the prevalence of sugar sweetened beverages consumption in subjects between 5 and 17 years old was 85.3%, and the average frequency of consumption was 0.71 times/day. The relationship between age and the prevalence of consumption displayed a J form, and the association with frequency in times/day was linear. The highest consumption occurred at 16 years of age, with a prevalence of 90.4% and an average frequency of 0.83 times/day. Overweight and obesity were not associated with consumption (p>0.05). Conclusions: the prevalence and average frequency of consumption were positively associated with age, wealth, and level of urbanism and conversely associated with education and household food security. Children with stunting have a reduced prevalence but increased frequency of consumption. The consumption of sweetened-beverages is an expression of the stage of nutritional and food transitions.


Resumo Objetivos: descrever o consumo de bebidas adoçadas com açúcar na Colômbia, América do Sul e as variáveis associadas a esse consumo. Métodos: embasados no Questionário de Frequência Alimentar aplicado na Pesquisa Nacional de Situação Nutricional da Colômbia (2010), foi estimada a prevalência e frequência/dia do consumo de bebidas adoçadas com açúcar por 10.070 indivíduos entre5 e 17 anos de idade. Resultados: a prevalência do consumo de bebidas adoçadas com açúcar em indivíduos entre 5 e 17 anos de idade foi de 85,3%, e a frequência média de consumo foi de 0,71 vezes/dia. A relação entre idade e prevalência de consumo demonstrou uma forma J, e a associação com a frequência em vezes/dia foi linear. O maior consumo ocorreu aos 16 anos de idade, com uma prevalência de 90,4% e uma frequência média de 0,83 vezes/dia. Sobrepeso e obesidade não foram associados ao consumo (p>0,05). Conclusões: a prevalência e a frequência média de consumo foram positivamente associadas com idade, condições financeiras e nível de urbanismo e inversamente associadas com educação e segurança alimentar doméstica. Crianças com déficit de crescimento têm prevalência reduzida, mas frequência de consumo aumentada. O consumo de bebidas adoçadas é uma expressão do estágio de transições nutricionais e alimentares.


Subject(s)
Humans , Child , Adolescent , Carbonated Beverages , Eating , Colombia , Juices , Dietary Sugars , Food and Nutrition Education , Nutritional Status , Food Security , Feeding Behavior
14.
Hig. aliment ; 31(268/269): 68-72, 30/06/2017.
Article in Portuguese | LILACS | ID: biblio-846449

ABSTRACT

Objetivou-se, neste estudo, avaliar as condições higienicossanitárias da cadeia produtiva do açaí desde a colheita até o processamento da polpa realizada na região do Maracanã, São Luís-MA enfatizando os processos de colheita do fruto, debulha, armazenamento, transporte e extração da polpa para comercialização. Concomitantemente, buscou-se avaliar se os produtores envolvidos tinham conhecimento sobre os agentes etiológicos de doenças alimentares, em especial da Doença de Chagas, cujo agente é transmitido por via oral pelo consumo do açaí. Para esta avaliação foi elaborado um questionário com 30 perguntas, baseado no Manual de Práticas de Colheita e Manuseio do Açaí proposto pela Embrapa (2006) e a aplicação se deu por meio de entrevistas pessoais. Os resultados com relação às medidas de higiene que devem ser adotadas durante a preparação da polpa de açaí, foram classificados como satisfatórios na sua maioria, conforme o manual, entretanto, os conhecimentos sobre a transmissão da doença de Chagas pelo açaí, foram escassos. Deste modo, é fundamental que atividades de conscientização devem ser desenvolvidas na comunidade para corroborar na prevenção desta e de outras doenças.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Euterpe/parasitology , Food Contamination/analysis , Food Parasitology , Food Production , Juices , Brazil , Chagas Disease/transmission , Food Handling , Foodborne Diseases , Public Health , Surveys and Questionnaires/statistics & numerical data
15.
Hig. aliment ; 31(268/269): 134-138, 30/06/2017.
Article in Portuguese | LILACS | ID: biblio-846503

ABSTRACT

Amostras de açaí (Euterpe oleracea Mart.) tipo fino congeladas e sem adição de quaisquer outros ingredientes foram adquiridas no comércio varejista da cidade do Rio de Janeiro. Foram analisadas microbiologicamente 54 amostras, pertencentes a 6 diferentes marcas (A, B, C, D, E e F). Todas as amostras analisadas encontravam-se dentro do prazo de validade estabelecido pelo fabricante e as empresas eram registradas no MAPA. Os micro-organismos analisados foram: Salmonella, Escherichia coli, fungos filamentosos e leveduras, de acordo com o preconizado pela legislação brasileira vigente. Considerando-se o limite máximo possível estabelecido pela lei, 2 lotes de cada marca B, E e F e 1 lote de cada marca C e D apresentaram contagens acima do permitido para fungos filamentosos e leveduras. Apenas 2 amostras apresentaram contagens de Escherichia coli, ambas estando fora do máximo estabelecido pela legislação vigente. Constatou- -se ausência de Salmonella em todas as amostras analisadas. A presença de fungos filamentosos, leveduras e Escherichia coli pode ser um indicativo de práticas inadequadas durante a fabricação do açaí. Assim, é importante ressaltar a necessidade de um controle rigoroso da cadeia do frio, uma vez que esses micro-organismos podem ser responsáveis pela deterioração do produto.


Subject(s)
Humans , Euterpe/microbiology , Food Contamination/analysis , Food Microbiology , Industrialized Foods , Juices , Coliforms , Escherichia coli/isolation & purification , Frozen Foods
16.
Acta sci., Biol. sci ; 39(2): 195-200, abr.- jun. 2017. tab
Article in English | LILACS | ID: biblio-846930

ABSTRACT

The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all juice samples analyzed were cytotoxic, genotoxic and mutagenic to root meristem cells. These results indicate that such beverages have relevant potential to cause cellular disorders and, thus, need to be evaluated more fully in more complex test systems, as those in rodents, and then establish specific toxicity at the cellular level of these juices and ensure the well-being of those who consume them.


Objetivou-se neste trabalho avaliar a toxicidade aguda em nível celular de sucos industrializados prontos para beber, sabor laranja e uva, de cinco empresas alimentícias de reconhecida reputação no mercado de alimentos em países da América do Sul, especialmente o Brasil. Esta avaliação se deu por meio das células meristemáticas de raízes de Allium cepa L., nos tempos de exposição 24 e 48h, diretamente nos preparados líquidos comercializados. Com base nos resultados obtidos verificou-se que os sucos de frutas, de todas as empresas consideradas, promoveram expressivo efeito antiproliferativo aos meristemas de raízes já no tempo de exposição 24h, e ocasionaram número estatisticamente significativo de alterações de fuso mitótico e quebras cromossômicas nas células do tecido analisado em todo o tempo de análise. Portanto, nas condições de estudo estabelecidas, os sucos das empresas avaliadas foram citotóxicos, genotóxicos e mutagênicos. Estes resultados são importantes em razão de indicarem que tais alimentos têm relevante potencial em causar distúrbios celulares e, portanto, devem ser avaliados em sistemas com testes mais complexo, como os em roedores, para, dessa forma, estabelecer com propriedade a toxicidade em nível celular desses alimentos e assegurar o bem-estar daqueles que os consomem.


Subject(s)
Cytotoxicity Tests, Immunologic , Genotoxicity , Industry , Juices , Mutagenicity Tests
17.
Diaeta (B. Aires) ; 35(159): 37-44, abr.-mayo 2017. tab
Article in Spanish | LILACS | ID: biblio-868864

ABSTRACT

Introducción: En Argentina un 37,1% de la población padece sobrepeso, un 20,8% obesidad y un 9,8% diabetes. La prevalencia de síndrome metabólico oscila entre el 20 y 25%. Según el estudio HIDRATAR, el consumo promedio de agua, bebidas e infusiones era de 2.050 ml/día, de los cuales un 29% correspondió a bebidas endulzadas artificialmente (BEA). No existe consenso de los organismos internacionales para su consumo. Metodología: Se llevó a cabo una búsqueda bibliográfica en PubMed, Scielo y Cochrane, de artículos publicados a partir del año 2005, con el objetivo de conocer la relación entre el consumo de BEA y el riesgo de desarrollo de síndrome metabólico y diabetes mellitus tipo 2. Resultados: Se encontraron 12 trabajos que señalan los efectos adversos de las BEA. Los mismos representan una mayor población en estudio, seguida durante más tiempo. Es un tema controvertido con mucha disparidad en la obtención de la información, teniendo en cuenta además que, en el caso de estudios con humanos, son muchos los componentes del plan alimentario que hay que considerar. Conclusiones: Ha quedado demostrado que los edulcorantes no nutritivos no son sustancias metabólicamente inertes y hay evidencia que sugiere que las BEA no son completamente inocuas, siendo primordial y necesaria la educación para limitar su consumo y promover la ingesta de agua.


Subject(s)
Humans , Carbonated Beverages/adverse effects , Diabetes Mellitus , Non-Nutritive Sweeteners/adverse effects , Juices , Metabolic Syndrome
18.
Rev. Asoc. Odontol. Argent ; 104(4): 166-174, oct.-dic. 2016. tab
Article in Spanish | LILACS | ID: biblio-869381

ABSTRACT

Objetivo: analizar bebidas y alimentos de consumo frecuente para evaluar el pH, grados Brix (ºBx), efecto buffer a pH 5,5 y 7, y concentración de fosfatos, calcio y fluoruros. Materiales y métodos: en este estudio observacional, descriptivo y transversal, se evaluaron 438 alimentos, divididos en 18 grupos. Se analizó pH, capacidad Buffer a pH 5,5 y 7, grados Brix y concentración de fosfatos, de fluoruros y de calcio. Los datos fueron procesados por SPSS Statistics 20 (IBM Corp., Estados Unidos). Resultados: las gaseosas, el jugo en polvo para diluir, las aguas saborizadas y las bebidas isotónicas presntaron menor pH. El rango de sólidos solubles, expresado en grados Brix, fue de 0,05 a 15,9. El mayor valor de efecto buffer correspondió a los jugos: exprimido, en polvo, listo para consumir y para diluir. En este último, se halló mayor cantidad de fosfato. Sólo en las aguas minerales con gas se observaron fluoruros. El alimento de soja líquido natural presentó el mayor contenido de calcio. Concusiójn: las gaseosas, los jugos, las leches fermentadas y las bebidas isotónicas, resultaron ser ácidas, con bajo efecto buffer, alto valor de grados Brix y contener pocos factores protectores.


Aims: to analyze drinks and foods commonly consumedby evaluating pH, degrees Brix, buffer effect at pH 5.5 and 7.0and phosphate, calcium and fluoride concentration.Materials and methods: In this observational, descriptiveand cross-sectional study, 48 foods were evaluated,divided into 18 groups. pH, buffer capacity at 5.5 and 7, degreesBrix and phosphate, fluoride and calcium concentrationwere analyzed. The data were processed by SPSS Statistics 20(IBM Corp., USA).Results: Soda, juice powder to dilute, flavored water andsports drinks had lower pH. The range of soluble solids (degreesBrix) was from 0.05 to 15.9. The greatest value in buffereffect was in juices: squeezed, powdered, ready to consumeand to be diluted. The latter had a higher amount of phosphate.Only sparkling mineral waters showed detectable valuesfor fluorides. The liquid natural soy food had the highestcalcium content.Conclusion: Soft drinks, juices, fermentable milk, isotonicdrinks were found to be acidic, with lower buffer value,high Brix degrees and low in protective factors.


Subject(s)
Humans , Food and Beverages/analysis , Dental Caries/etiology , Risk Factors , Tooth Erosion , Breast-Milk Substitutes , Carbonated Beverages , Cross-Sectional Studies , Calcium/analysis , Fluorides/analysis , Phosphates/analysis , Juices , Mineral Waters , Soy Milk , Statistical Analysis
19.
Actual. nutr ; 17(4): [128]-[133], dic 2016.
Article in Spanish | LILACS | ID: biblio-970637

ABSTRACT

Las interacciones fármaco-nutriente (F-N) tienen el potencial de alterar significativamente la eficacia y seguridad de las terapias farmacológicas. Esta revisión se enfoca en las interacciones del jugo de pomelo con fármacos selectos. Los mecanismos involucrados incluyen la inhibición del sistema metabolizador microsomal CYP3A y del transportador glicoproteína P en el enterocito. El conocimiento y la evitación de estas interacciones clínicamente relevantes aumentarán la seguridad del paciente


Interactions between drugs and nutrients (F-N) have the potential to significantly alter the efficacy and safety of pharmacological therapies. This review focuses on the interactions between grapefruit juice and selected drugs. Involved mechanisms include the inhibition of both CYP 3A4 microsomal metabolizing enzymes and P glycoprotein transporter within the enterocyte. Knowledge (and avoidance) of this clinically relevant interactions will enhance patient safety


Subject(s)
Humans , Pharmaceutical Preparations , Food-Drug Interactions , Citrus paradisi , Juices
20.
Hig. aliment ; 30(254/255): 123-127, 30/04/2016.
Article in Portuguese | LILACS | ID: biblio-836657

ABSTRACT

qualidade de polpas de frutas simples comercializadas na cidade de Juazeiro do Norte, Ceará, através da identificação de Staphylococcus aureus, Salmonella spp e Escherichia coli. As amostras coletadas foram processadas e semeadas em Ágar Manitol, Ágar EMB e Ágar SS. Submeteu-se as colônias sugestivas às provas bioquímicas para confirmação da espécie. Analisou-se um total de 40 amostras de polpas de frutas, sendo essas de acerola, abacaxi, cajá, goiaba, graviola, mamão, manga e maracujá. Nesta pesquisa, foi identificada Salmonella spp em duas amostras, o que contraria as normas vigentes onde este micro- -organismo não deve estar presente. Staphylococcus aureus estava presente em 31 amostras, não observando a presença de Escherichia coli. Dessa forma, conclui-se que as polpas de frutas apresentaram uma alta porcentagem de contaminação microbiológica remetendo a falhas durante seu processamento como condições higienicossanitárias deficientes dos instrumentos e falta de instrução dos manipuladores.


Subject(s)
Humans , Food Contamination/analysis , Food Microbiology , Fruit/microbiology , Juices , Brazil , Escherichia coli/isolation & purification , Food Samples , Foodborne Diseases , Staphylococcus aureus/isolation & purification
SELECTION OF CITATIONS
SEARCH DETAIL