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1.
Prensa méd. argent ; 107(8): 418-422, 20210000. fig
Article in Spanish | LILACS, BINACIS | ID: biblio-1358673

ABSTRACT

Las betalactamasas de espectro extendido (BLEE) son enzimas producidas por bacilos gram negativos capaces de hidrolizar las cefalosporinas de amplio espectro y los monobactámicos. La mayoría pertenece a la familia de Enterobacteriae, tales como Klebsiella pneumoniae y Escherichia coli: Sin embargo, se asocian también con otras bacterias como Proteus, Serratia, Salmonella, Pseudomonas aeruginosa y Acinetobacter. Las enterobacterias productoras de carbapenemasas no sólo han sido aisladas en el ambiente hospitalario, sino que también provienen de la comunidad. Se presenta una paciente de sexo femenino con antecedentes de sida y osteomielitis secundaria a artritis séptica producida por una Klebsiella pneumoniae BLEE de la comunidad. Un tratamiento oportuno y eficaz puede evitar la opción quirúrgica, disminuyendo la morbimortalidad asociada con esta afección


Extended-spectrum beta-lactamases (ESBL) are enzymes produced by gram-negative rods capable of hydrolyzing broad-spectrum cephalosporins and monobactams. Most belong to the Enterobacteriae family, such as Klebsiella pneumoniae and Escherichia coli. However, they are also associated with other bacteria such as Proteus, Serratia, Salmonella, Pseudomonas aeruginosa and Acinetobacter. Carbapenemase-producing Enterobacteriaceae have not only been isolated from the hospital environment, but also from the community. We present a female patient with a history of AIDS and secondary osteomyelitis to septic arthritis caused by a community Klebsiella pneumoniae ESBL. It is concluded that a timely and effective treatment can avoids the surgical option, reducing the morbidity and mortality of this condition.


Subject(s)
Humans , Female , Adult , Osteomyelitis/immunology , Klebsiella Infections/therapy , Arthritis, Infectious/therapy , Imipenem/therapeutic use , AIDS-Related Opportunistic Infections/immunology , Arthrocentesis , Knee Injuries/therapy
3.
Article in Portuguese | LILACS | ID: biblio-1359491

ABSTRACT

RESUMO: As infecções associadas aos Dispositivos Cardíacos Eletrônicos Implantáveis (DCEI) apresentam uma incidência de até 3,4% e notável impacto na morbidade e mortalidade dos pacientes. As bactérias Gram-positivas, especialmente do gênero Staphylococcus sp. representam 60-70% dos agentes isolados. Por sua vez, as Gram-negativas correspondem até 9% dos casos. Relatamos uma infecção de loja de gerador de Cardioversor-desfibrilador implantável (CDI) por uma Klebsiella sp. resistente aos carbapenêmicos, em um paciente masculino jovem, cujo desafiador diagnóstico de certeza desse caso somente foi possível após exploração cirúrgica e cultura do material da loja do CDI, haja vista a apresentação clínica oligossintomática. Embora já descritas, Klebsiella sp. são raras nesse contexto e em nosso conhecimento, esse é o primeiro relato de uma infecção de DCEI por uma enterobactéria resistente a carbapenêmico. (AU)


ABSTRACT: Infections associated with Implantable Electronic Cardiac Devices (IECD) have an incidence of up to 3.4% and a notable impact on patient morbidity and mortality. Gram-positive bacteria, especially Staphylococcus sp. represent 60-70% of isolated agents. In turn, gram-negatives account for up to 9% of cases. We report an Implantable Cardioverter-Defibrillator (ICD) generator pocket infection by a Carbapenem Resistant Klebsiella sp., in a young male patient, whose challenging diagnosis of certainty was only possible after surgical exploration and culture of the material from the ICD pocket, given the oligosymptomatic clinical presentation. Although already described, Klebsiella sp. are rare in this context and to our knowledge, this is the first report of an IECD infection by a carbapenem-resistant enterobacterium. (AU)


Subject(s)
Humans , Male , Adult , Klebsiella Infections , Defibrillators, Implantable , Carbapenem-Resistant Enterobacteriaceae
4.
Rev. chil. infectol ; 37(5): 566-569, nov. 2020. tab
Article in Spanish | LILACS | ID: biblio-1144252

ABSTRACT

Resumen Introducción: Los abscesos hepáticos primarios producidos por Klebsiella pneumoniae y las implicancias sistémicas relacionadas constituyen el síndrome de absceso hepático invasor por Klebsiella pneumoniae. Objetivo: Describir las características clínicas, epidemiológicas y la evolución de esta entidad en nuestro centro. Pacientes y Métodos: Cohorte retrospectiva de pacientes adultos internados en el Hospital Italiano de Buenos aires entre el 1 de enero de 2001 y el 1 de mayo de 2020. Se incluyeron pacientes con diagnóstico de absceso en cualquier órgano con aislamiento en cultivo de absceso o hemocultivo positivo para Klebsiella pneumoniae para ser analizadas las características epidemiológicas, clínicas y la evolución de aquellos con localización primaria hepática. Resultados: 10 pacientes fueron incluidos. Dos (20%) pacientes fueron orientales. La mediana de edad fue 69 años (IIC 64-79), nueve (90%) fueron hombres. La co-morbilidad más frecuente fue diabetes mellitus tipo 2 (40%). Cuatro (40%) pacientes tuvieron diseminación a otros órganos. La mediana de internación fue 21,5 días (IIC 15-43), 60% (n: 6) requirió internación en unidad de cuidados intensivos y 30% (n: 3) de los pacientes falleció. Conclusión: El síndrome de absceso hepático invasor por Klebsiella pneumoniae es una enfermedad infrecuente, potencialmente mortal. Este estudio intenta reportar las características de los pacientes con esta patología en nuestra población.


Abstract Background: Primary liver abscesses caused by Klebsiella pneumoniae and their related systemic complications produce the invasive liver abscess syndrome due to Klebsiella pneumoniae. Aim: To describe the clinical, epidemiological and evolution characteristics in our center. Methods: A retrospective cohort of hospitalized adults in Hospital Italiano de Buenos Aires between January 1st, 2001 and May 1st, 2020. We included patients with diagnosis of abscess in any organ with rescue in culture or positive blood culture for Klebsiella pneumoniae. Epidemiological, clinical characteristics and prognosis of those with hepatic primary localization were analyzed. Results: 10 patients were included. Two (20%) patients were Oriental. Median age was 69 years (interquartile range 64-79), nine (90%) were men. The most frequent comorbidity was type 2 diabetes (40%). Four (40%) patients had spread to other organs. The median hospitalization was 21.5 days (IIC 15-43), 60% (n: 6) were hospitalized in the intensive care unit and 30% (n: 3) died. Conclusions: The invasive liver abscess syndrome due to Klebsiella pneumoniae is a rare life-threatening disease. Our study reports the characteristics of patients with this syndrome in our population.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Klebsiella Infections/diagnosis , Klebsiella Infections/epidemiology , Liver Abscess/epidemiology , Retrospective Studies , Diabetes Mellitus, Type 2 , Klebsiella pneumoniae
5.
Rev. cuba. invest. bioméd ; 39(3): e612, jul.-set. 2020. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1138925

ABSTRACT

Introducción: Las infecciones asociadas a los cuidados sanitarios en los servicios de atención al paciente crítico se asocian a un alto riesgo de muerte y costos significativos. Objetivo: Identificar los gérmenes más frecuentes en los cultivos y su resistencia a los antimicrobianos en la terapia intensiva del Hospital Clínico Quirúrgico Provincial Dr. Joaquín Albarrán. Métodos: Se realizó un estudio descriptivo, retrospectivo, en la terapia intensiva del Hospital Clínico Quirúrgico Provincial Dr. Joaquín Albarrán, de enero del 2015 a diciembre del 2018, el universo estuvo constituido por 1847 cultivos realizados seleccionándose 654 en los que se obtuvo crecimiento; para el análisis estadístico se utilizó el SPSS 22.0. Resultados: De los gérmenes aislados, la Klebsiella spp fue la más frecuente (31 por ciento), seguida del Staphylococcus spp (24,5 por ciento) y de la E. coli (9,8 por ciento). En los esputos se mantuvo la Klebsiella spp (45,1 por ciento), en los hemocultivos el Staphylococcus spp (53,6 por ciento) y en los urocultivos la Candida (41,1 por ciento), seguida de la E. coli (27 por ciento). De los antimicrobianos usados en terapia para tratar la Klebsiella spp, la E. coli, el Acinetobacter y la pseudomona spp, el más recomendado es la colistina (0-25 por ciento de resistencia) y para el Staphylococcus spp, la vancomicina (1,8 por ciento). Conclusiones: Los gérmenes Gram negativos siguen siendo los más frecuentemente aislados en los cultivos de los pacientes hospitalizados en terapia, con un alto nivel de resistencia para la mayoría de los antibióticos(AU)


Introduction: Infections related to critical care settings are associated to high death risk and significant costs. Objective: Identify the germs most commonly found in cultures and their resistance to antimicrobials in the intensive care service of Dr. Joaquín Albarrán Provincial Clinical Surgical Hospital. Methods: A retrospective descriptive study was conducted at the intensive care service of Dr. Joaquín Albarrán Provincial Clinical Surgical Hospital from January 2015 to December 2018. The study universe was 1 847 cultures, from which 654 were selected in which growth was obtained. Data were processed with the statistical software SPPS 22.0. Results: Of the germs isolated, Klebsiella spp. were the most common (31 percent, followed by Staphylococcus spp. (24.5 percent) and E. coli (9.8 percent). Klebsiella spp. were frequent in sputum cultures (45.1 percent), Staphylococcus spp. in blood cultures (53.6 percent) and Candida in urine cultures (41.1 percent), followed by E. coli (27 percent). Among the antimicrobials used to treat Klebsiella spp., E. coli, Acinetobacter and Pseudomonas spp., the most recommended is colistin (0-25 percent resistance) and vancomycin for Staphylococcus spp. (1.8 percent). Conclusions: Gram-negative germs continue to be the most commonly isolated in cultures from intensive care patients, with a high level of resistance to most antibiotics(AU)


Subject(s)
Humans , Male , Female , Klebsiella Infections/prevention & control , Vancomycin/therapeutic use , Cross Infection/prevention & control , Colistin/therapeutic use , Intensive Care Units/statistics & numerical data , Epidemiology, Descriptive , Retrospective Studies
6.
Rev. chil. infectol ; 37(4): 362-370, ago. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1138560

ABSTRACT

Resumen Introducción: Las enterobacterias son una causa principal de infecciones del torrente sanguíneo y su resistencia antimicrobiana se encuentra en aumento. Esto lleva a un incremento de la morbilidad-mortalidad y de los costos en la salud pública. Las enterobacterias resistentes a carbapenems representan un grave desafío a nivel global ya que existen escasas opciones terapéuticas disponibles. Objetivo: Caracterización clínico/microbiológica de las bacteriemias resistentes a carbapenémicos observadas en un período de 4 años. Material y Método: Estudio retrospectivo, observacional y descriptivo, sobre las bacteriemias por enterobacterias resistentes y sensibles a carbapenems. Resultados: Se analizó un total de 84 pacientes con bacteriemia por enterobacterias resistentes y sensibles a carbapenems. Entre las resistentes, observamos una mayor proporción de: tratamiento antimicrobiano previo, hospitalización en unidad de terapia intensiva (UTI), inicio de la bacteriemia en UTI y antecedentes de β-lactamasas de espectro extendido. Además, se detectó un amplio predominio de Klebsiella pneumoniae productor de KPC y una mortalidad atribuible de 52,4%. Discusión: El estudio posibilitó profundizar el conocimiento de una enfermedad emergente de elevada mortalidad, en vistas al diseño y aplicación de estrategias de control de infecciones y de esquemas de tratamiento efectivos adaptados a la epidemiologia local.


Abstract Background: Enterobacteriaceae are a major cause of bloodstream infections and their antimicrobial resistance continues to increase. This leads to higher morbidity-mortality rates and public health costs. Carbapenem-resistant Enterobacteriaceae represent a serious challenge globally, since there are few therapeutic options available. Aim: Clinical/microbiological characterization of the carbapenem-resistant bacteremia observed over a period of 4 years. Methods: Retrospective, observational and descriptive study about bacteremia caused by carbapenem-resistant and susceptible Enterobacteriaceae. Results: A total of 84 patients with bacteremia including carbapenem-resistant and susceptible Enterobacteriaceae were analyzed. We found that patients infected with carbapenem-resistant strains presented a higher proportion of: previous antibiotic treatment, hospitalization in intensive care unit (ICU), onset of the bacteremia during hospitalization in ICU and previous infection with extended-spectrum-beta-lactamase producing Enterobacteriaceae. Additionally, we observed a predominance of KPC-producing Klebsiella pneumoniae and an attributable mortality rate of 52.4%. Discussion: This study allowed for a better understanding of an emerging problem with high mortality, which in turn is useful for the design and adoption of infection control strategies and effective treatment regimens adapted to our local epidemiology.


Subject(s)
Humans , Klebsiella Infections/drug therapy , Klebsiella Infections/epidemiology , Bacteremia/drug therapy , Bacteremia/epidemiology , Argentina/epidemiology , beta-Lactamases , Microbial Sensitivity Tests , Carbapenems/pharmacology , Retrospective Studies , Drug Resistance, Bacterial , Enterobacteriaceae , Klebsiella pneumoniae , Anti-Bacterial Agents/therapeutic use , Anti-Bacterial Agents/pharmacology
7.
Rev. ecuat. pediatr ; 21(2): 1-11, 31 de agosto del 2020.
Article in Spanish | LILACS | ID: biblio-1141081

ABSTRACT

Introducción: Las infecciones nosocomiales ocasionan un costo para el sistema de salud. En este estudio se reporta los datos observacionales de un brote de Klebsiella Pneumoniae en una Unidad de neonatología, y sus medidas posteriores a la identificación. Métodos: Con un diseño descriptivo observacional, ambispectivo se estudiaron casos de infecciones nosocomiales. Se realizaron medidas de barrera estrictas con listas de chequeo y uso de cámaras de vigilancia en una Unidad de Neonatología. Se reportan las observaciones del estudio. Se utiliza estadística descriptiva. Resultados: 6 casos fueron diagnosticados con Klebsiella Pneumoniae, todos fueron pacientes recién nacidos prematuros, tres de ellos extremos. Cuatro pacientes con sepsis temprana. Las muestras de cultivos fueron 3 Hemocultivos, 2 Urocultivos y 1 cultivo de secreción faríngea. Junto con el cultivo de Klebsiella pneumoniae, se identificaron otros patógenos. La coinfección de Klebsiella con S. aureus fue la que se mantuvo más días en aislamiento, en relación al aislamiento de Klebsiella sola. De todos los cultivos, 2 fueron positivos para Klebsiella pneumoniae productora de BLEE, y cuatro para Klebsiella pneumoniae multisensible. Luego de la intervención no se presentaron nuevo casos con un seguimiento de 12 meses posteriores al brote. Conclusiones: Luego del brote de 6 casos de Klebsiella Pneumoniae en la unidad de Neonatología se establecieron medidas estrictas de bioseguridad, las mismas que fueron cumplidas con el registro en listas de chequeo y monitorizadas por cámaras de seguridad. Se logró la eliminación de infecciones nosocomiales en un período de control posterior a 12 meses luego de la implementación


Introduction: Nosocomial infections cause a cost for the health system. This study reports the observational data of an outbreak of Klebsiella Pneumoniae in a neonatology unit, and its measures after identification. Methods: With a descriptive, observational, ambispective design, nosocomial infections were studied. Strict barrier measures were carried out with checklists and the use of surveillance cameras in a Neonatology Unit. Study observations are reported. Descriptive statistics are used. Results: 6 cases were diagnosed with Klebsiella Pneumoniae, all were premature newborn patients, three of them extreme. Four patients with early sepsis. The culture samples were 3 blood cultures, 2 urine cultures and 1 culture of pharyngeal secretion. Along with the Klebsiella pneumoniae culture, other pathogens were identified. Klebsiella co-infection with S. aureus was the one that remained in isolation for more days, in relation to Klebsiella isolation alone. Of all the cultures, 2 were positive for ESBL-producing Klebsiella pneumoniae, and four for multisensitive Klebsiella pneumoniae. After the intervention, no new cases were presented with a follow-up of 12 months after the outbreak. Conclusions: After the outbreak of 6 cases of Klebsiella Pneumoniae in the Neonatology unit, strict biosafety measures were established, which were complied with with the registration in checklists and monitored by security cameras. Elimination of nosocomial infections was achieved in a control period after 12 months after implementation


Subject(s)
Humans , Klebsiella Infections , Klebsiella pneumoniae , Neonatology , Intensive Care Units, Neonatal , Critical Care
8.
Chinese Medical Journal ; (24): 2573-2585, 2020.
Article in English | WPRIM | ID: wpr-877863

ABSTRACT

BACKGROUND@#Carbapenemase-producing Klebsiella pneumoniae (CP-Kp) poses distinct clinical challenges due to extensively drug resistant (XDR) phenotype, and sequence type (ST) 11 is the most dominant blaKPC-2-bearing CP-Kp clone in China. The purpose of this current retrospective study was to explore the genetic factors associated with the success of XDR CP-Kp ST11 strains circulated in the intensive care unit (ICU) of a Chinese tertiary hospital.@*METHODS@#Six ST11 XDR CP-Kp strains were identified between May and December 2014 and validated by minimum inhibitory concentration examination, polymerase chain reaction, and pyrosequencing. The six ST11 XDR CP-Kp, as well as three multi-drug resistant (MDR) and four susceptible strains, were sequenced using single-molecule real-time method. Comprehensively structural and functional analysis based on comparative genomics was performed to identify genomic characteristics of the XDR ST11 CP-Kp strains.@*RESULTS@#We found that ST11 XDR blaKPC-2-bearing CP-Kp strains isolated from inpatients spread in the ICU of the hospital. Functionally, genes associated with information storage and processing of the ST11 XDR CP-Kp strains were more abundant than those of MDR and susceptible strains, especially genes correlative with mobile genetic elements (MGEs) such as transposons and prophages. Structurally, eleven large-scale genetic regions taken for the unique genome in these ST11 XDR CP-Kp strains were identified as MGEs including transposons, integrons, prophages, genomic islands, and integrative and conjugative elements. Three of them were located on plasmids and eight on chromosomes; five of them were with antimicrobial resistance genes and eight with adaptation associated genes. Notably, a new blaKPC-2-bearing ΔΔTn1721-blaKPC-2 transposon, probably transposed and truncated from ΔTn1721-blaKPC-2 by IS903D and ISKpn8, was identified in all six ST11 XDR CP-Kp strains.@*CONCLUSION@#Our findings suggested that together with clonal spread, MGEs identified uniquely in the ST11 XDR CP-Kp strains might contribute to their formidable adaptability, which facilitated their widespread dissemination in hospital.


Subject(s)
Anti-Bacterial Agents , Bacterial Proteins , China , Electrophoresis, Gel, Pulsed-Field , Hospitals , Humans , Klebsiella Infections/drug therapy , Klebsiella pneumoniae/genetics , Microbial Sensitivity Tests , Multilocus Sequence Typing , Pharmaceutical Preparations , Retrospective Studies , beta-Lactamases/genetics
9.
Article in Chinese | WPRIM | ID: wpr-878679

ABSTRACT

Objective To explore the prognostic factors of central venous catheter-related bloodstream infection(CR-BSI)and provide reference for clinical practice. Methods The clinical data of 346 CR-BSI patients from February 2014 to July 2019 were retrospectively reviewed,and the prognostic factors were analyzed. Results Of the 346 CR-BSI patients,62 died,yielding a case-fatality rate of 17.92%.Univariate analysis showed that 18 factors including age(


Subject(s)
Anti-Bacterial Agents , Carbapenem-Resistant Enterobacteriaceae , Central Venous Catheters/adverse effects , Humans , Hyperglycemia , Hypoproteinemia , Klebsiella Infections , Klebsiella pneumoniae , Methicillin-Resistant Staphylococcus aureus , Mycoses , Prognosis , Pseudomonas Infections , Retrospective Studies , Risk Factors , Sepsis/mortality
10.
Rev. Soc. Bras. Med. Trop ; 53: e20200032, 2020. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1136877

ABSTRACT

Abstract INTRODUCTION: Essential oils can serve as novel sources of antibiotics for multidrug-resistant bacteria. METHODS: The multidrug-resistance profile of a Klebsiella aerogenes strain was assessed by PCR and sequencing. The antibacterial activity of Cinnamomum cassia essential oil (CCeo) against K. aerogenes was assessed by broth microdilution and time-kill methods. RESULTS: K. aerogenes showed high antibiotic resistance. The genes bla KPC-2, ampC, bla CTX-M-15, bla OXA-1, and bla TEM were present. CCeo exhibited an inhibitory effect with a minimum inhibitory concentration of 17.57 μg/mL. CONCLUSIONS: The antibacterial activity of CCeo makes it a potential candidate for treating carbapenem- and polymyxin-resistant K. aerogenes strains.


Subject(s)
Humans , Klebsiella Infections/drug therapy , Enterobacter aerogenes , Cinnamomum aromaticum , Anti-Bacterial Agents/therapeutic use , beta-Lactamases , Oils, Volatile , Carbapenems , Polymyxins , Klebsiella pneumoniae
11.
Rev. Soc. Bras. Med. Trop ; 53: e20200064, 2020. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1136871

ABSTRACT

Abstract INTRODUCTION: Carbapenem-resistant Klebsiella pneumoniae infection lacks treatment options and is associated with prolonged hospital stays and high mortality rates. The production of carbapenemases is one of the most important factors responsible for this multi-resistance phenomenon. METHODS: In the present study, we analyzed the presence of genes encoding carbapenemases in K. pneumoniae isolates circulating in one of the public hospitals in the city of Aracaju, Sergipe, Brazil. We also determined the best combination of drugs that display in vitro antimicrobial synergy. First, 147 carbapenem-resistant K. pneumoniae isolates were validated for the presence of blaKPC, bla GES, bla NDM, bla SPM, bla IMP, bla VIM, and bla OXA-48 genes using multiplex polymerase chain reaction. Thereafter, using two isolates (97 and 102), the role of double and triple combinational drug therapy as a treatment option was analyzed. RESULTS: Seventy-four (50.3%) isolates were positive for bla NDM, eight (5.4%) for bla KPC, and one (1.2%) for both bla NDM and bla KPC. In the synergy tests, double combinations were better than triple combinations. Polymyxin B and amikacin for isolate 97 and polymyxin B coupled with meropenem for isolate 102 showed the best response. CONCLUSIONS: Clinicians in normal practice use multiple drugs to treat infections caused by multi-resistant microorganism; however, in most cases, the benefit of the combinations is unknown. In vitro synergistic tests, such as those described herein, are important as they might help select an appropriate multi-drug antibiotic therapy and a correct dosage, ultimately reducing toxicities and the development of antibiotic resistance.


Subject(s)
Humans , Bacterial Proteins/genetics , beta-Lactamases/genetics , Klebsiella Infections/microbiology , Klebsiella pneumoniae/drug effects , Anti-Bacterial Agents/pharmacology , Brazil , DNA, Bacterial , Microbial Sensitivity Tests , Polymerase Chain Reaction , Prevalence , Drug Resistance, Multiple, Bacterial/genetics , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/genetics
12.
Rev. Soc. Bras. Med. Trop ; 53: e20190526, 2020. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1136834

ABSTRACT

Abstract INTRODUCTION: This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing. RESULTS: The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.


Subject(s)
Humans , beta-Lactamases/genetics , Klebsiella Infections/microbiology , Klebsiella pneumoniae/genetics , Anti-Bacterial Agents/pharmacology , Plasmids/genetics , DNA, Bacterial/genetics , Microbial Sensitivity Tests , Polymerase Chain Reaction , Drug Resistance, Multiple, Bacterial , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/enzymology
13.
Rev. Soc. Bras. Med. Trop ; 53: e20200397, 2020. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1136816

ABSTRACT

Abstract INTRODUCTION Antibiotic resistance in carbapenemase-producing Klebsiella pneumoniae is acquired and disseminated mainly by plasmids. Therefore, we aimed to investigate the occurrence of carbapenemase genes, analyze the genetic diversity by ERIC-PCR, and examine the most common plasmid incompatibility groups (Incs) in clinical isolates of K. pneumoniae from colonization and infection in patients from a hospital in Brazil. METHODS Twenty-seven isolates of carbapenem-resistant K. pneumoniae were selected and screened for the presence of carbapenemase genes and Incs by PCR, followed by amplicon sequencing. RESULTS The bla KPC and bla NDM genes were detected in 24 (88.8 %) and 16 (59.2 %) of the isolates, respectively. Thirteen isolates (48.1 %) were positive for both genes. The IncFIB (92.6 %) and IncQ (88.8 %) were the most frequent plasmids, followed by IncA/C, IncHI1B, and IncL/M, indicating that plasmid variability existed in these isolates. To our knowledge, this is the first report of IncHI1B in Brazil. We found eight isolates with clonal relationship distributed in different sectors of the hospital. CONCLUSIONS The accumulation of resistance determinants, the variability of plasmid Incs, and the clonal dissemination detected in K. pneumoniae isolates demonstrate their potential for infection, colonization, and the dissemination of different resistance genes and plasmids.


Subject(s)
Humans , Klebsiella Infections , Klebsiella pneumoniae/genetics , Plasmids/genetics , Bacterial Proteins/genetics , beta-Lactamases/genetics , Brazil , Microbial Sensitivity Tests , Hospitals, Public , Anti-Bacterial Agents/pharmacology
16.
Rev. bras. enferm ; 72(3): 760-766, May.-Jun. 2019. tab
Article in English | LILACS, BDENF | ID: biblio-1013564

ABSTRACT

ABSTRACT Objective: To evaluate the risk factors related to Klebsiella pneumoniae carbapenemase infection after renal transplantation. Methods: This was a retrospective epidemiological (case-control) study, conducted from October 2011 to march 2016. Transplanted patients with infection by this bacteria during hospitalization were selected as cases. The controls were paired by age, sex, type of donor and transplant time. The proportion of cases and controls was 1:2. Results: Thirty hundred and five patients were included in the study (45 cases and 90 controls). The risk factors found for infection by KPC were: time of hospitalization after the transplant (OR: 4.82; CI95% 2.46-9.44), delayed kidney function (OR: 5.60; CI95% 1.91-11.01) and previous infectious for another microorganism ( OR: 34.13 CI95% 3.52-132.00). Conclusion: The risk of acquisition of this bacterium was directly related to invasive procedures and exposure to the hospital environment. The findings reinforce the importance of prevention measures and control of infection by this microorganism.


RESUMEN Objetivo: Evaluar los factores de riesgo relacionados con la infección por Klebsiella pneumoniae carbapenemasa después del trasplante renal. Método: Estudio retrospectivo epidemiológico (caso-control), realizado de octubre de 2011 a marzo de 2016. Pacientes transplantados con infección por esa bacteria durante la internación fueron seleccionados como casos. Los controles se parearon por edad, sexo, tipo de donante y tiempo de trasplante. La proporción de casos y controles fue de 1: 2. Resultados: Treinta y cinco pacientes fueron incluidos en el estudio (45 casos y 90 controles). Los factores de riesgo para la infección encontrados por KPC fueron: tiempo de hospitalización después del trasplante (OR: 4,82, IC95% 2,46-9,44), función renal retardada (OR: 5,60, IC95% 1, 91-11,01) y anterior infecciosa para otro microorganismo (OR: 34,13 IC95% 3,52-132,00). Conclusión: El riesgo de adquisición de esta bacteria estuvo directamente relacionado a procedimientos invasivos y exposición al ambiente hospitalario. Los hallazgos refuerzan la importancia de medidas de prevención y control de la infección por ese microorganismo.


Subject(s)
Humans , Male , Female , Adult , Pneumonia/ethnology , Bacterial Proteins/adverse effects , beta-Lactamases/adverse effects , Klebsiella Infections/etiology , Kidney Transplantation/adverse effects , Pneumonia/chemically induced , Pneumonia/epidemiology , Bacterial Proteins/metabolism , beta-Lactamases/metabolism , Brazil/epidemiology , Klebsiella Infections/metabolism , Klebsiella Infections/epidemiology , Case-Control Studies , Retrospective Studies , Risk Factors , Kidney Transplantation/methods , Klebsiella pneumoniae/metabolism , Klebsiella pneumoniae/pathogenicity , Middle Aged
17.
Rev. Assoc. Med. Bras. (1992) ; 65(5): 678-681, May 2019. graf
Article in English | LILACS | ID: biblio-1012972

ABSTRACT

SUMMARY OBJECTIVE: We describe the case of a diabetic patient who developed vertebral osteomyelitis and bilateral psoas abscess with gas formation due to klebsiella pneumoniae. METHODS: A 64-year-old woman with a 4-year history of type-2 diabetes mellitus was admitted to the Emergency Department. The subject had a 2-day history of high-grade fever associated with chills and a 5-hour history of consciousness. She received empirical treatment with febrifuge, after which her fever decreased. RESULTS: Her fever recurred after an interval of three hours. A computed tomography scan of the abdomen revealed vertebral osteomyelitis and bilateral psoas muscle abscess with gas formation. Blood culture and purulent fluid described the growth of the Klebsiella pneumoniae. The patient received antibiotic therapy and bilateral drainage therapy after the drainage catheter was placed into the abscess cavity by CT-guidance. Due to the serious damage to the vertebral column and permanent pain, the patient underwent minimally invasive internal spinal fixation and recovered successfully. CONCLUSION: A case of vertebral osteomyelitis and bilateral psoas abscess with gas formation caused by Klebsiella pneumoniae in a diabetic patient. Antibiotic therapy, drainage, and minimally invasive internal spinal fixation were performed, which enabled a good outcome.


RESUMO OBJETIVO: Descrever o caso de uma paciente diabética que desenvolveu osteomielite vertebral e abcesso bilateral do psoas com formação de gás causada por klebsiella pneumoniae. MÉTODOS: Uma mulher de 64 anos de idade, com 4 anos de histórico de diabetes mellitus tipo 2, foi admitida no Serviço de Emergência. A paciente apresentava um quadro de dias de febre alta acompanhada de calafrios e um histórico de 5 horas de consciência. Ela recebeu tratamento empírico com antitérmico, após o qual a febre diminuiu. RESULTADOS: A febre retornou após um intervalo de três horas. Uma tomografia computadorizada do abdome revelou osteomielite vertebral e abcesso bilateral do músculo psoas com formação de gás. A cultura do sangue e o fluido purulento revelaram o crescimento de Klebsiella pneumoniae. A paciente recebeu antibióticos e terapia de drenagem bilateral após o cateter de drenagem ser posicionado na cavidade do abscesso com auxílio de TC. Devido a sérios danos à coluna vertebral e a dor permanente, a paciente foi submetida à fixação vertebral interna minimamente invasiva e recuperou-se com sucesso. CONCLUSÃO: Um caso de osteomielite vertebral e abscesso do psoas bilateral com a formação de gás causada por Klebsiella pneumoniae em uma paciente diabética. Antibioticoterapia, drenagem e fixação vertebral interna minimamente invasiva foram realizadas, o que permitiu um bom resultado.


Subject(s)
Humans , Female , Osteomyelitis/surgery , Spinal Diseases/surgery , Klebsiella Infections/surgery , Psoas Abscess/surgery , Diabetes Complications/surgery , Klebsiella pneumoniae/pathogenicity , Osteomyelitis/microbiology , Spinal Diseases/microbiology , Klebsiella Infections/microbiology , Tomography, X-Ray Computed/methods , Drainage/methods , Reproducibility of Results , Treatment Outcome , Psoas Abscess/microbiology , Diabetes Complications/microbiology , Gases/metabolism , Middle Aged
18.
Mem. Inst. Invest. Cienc. Salud (Impr.) ; 17(1): 59-68, abr. 2019. tab, ilus
Article in Spanish | LILACS, BDNPAR | ID: biblio-1008026

ABSTRACT

Las Infecciones Asociadas a las Atenciones Sanitarias (IAAS), constituyen un serio problema de salud pública. Se describe un brote de IAAS en una Unidad de Cuidados Intensivos Neonatales de Asunción y las medidas de control implementadas. Estudio descriptivo de serie de casos, entre el 26 de marzo y el 18 de abril del 2013. Fueron utilizados datos secundarios como historias clínicas, registros de enfermería, certificados de defunción, resultados de bacteriología, registros de control de IAAS. El universo estuvo constituido por 12 neonatos, en cuatro se aislaron Pseudomona aeruginosa y en tres Klebsiella pneumoniae BLEE. Predomino el sexo masculino (6/7), la media de edad de 2 días (rango:2-45 días), la mayoría prematuros (5/7). Todos con procedimientos invasivos durante la hospitalización. La tasa global de ataque fue 58% (7/12), con una tasa de ataque para P. aeruginosa de 33 % (4/12) y para K. pneumoniae BLEE de 38 % (3/12). La mortalidad global fue 29% (2/12) y la tasa de la letalidad atribuible a la infección por P. aeruginosa fue del 50% (2/4). Las medidas de control del brote fueron el aislamiento de los pacientes infectados, una enfermera por paciente, se intensifico los cuidados de bioseguridad para procedimientos invasivos y lavado de manos; suspensión de pacientes remitidos hasta que se asegurar el corte de transmisión. Se confirmó dos brotes simultáneos con agentes etiológicos diferentes, P. aeruginosa y K. pneumoniae BLEE. Es necesario reforzar las medidas de control de infecciones y lavado correcto de manos, de manera a prevenir brotes de IAAS en la UCIN(AU)


Subject(s)
Humans , Male , Female , Infant, Newborn , Infant , Pseudomonas aeruginosa , Pseudomonas Infections/epidemiology , Klebsiella Infections/epidemiology , Intensive Care Units, Neonatal , Cross Infection/epidemiology , Klebsiella pneumoniae , Paraguay/epidemiology , Disease Outbreaks
19.
Rev. cuba. med. gen. integr ; 35(1): e814, ene.-mar. 2019. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1093480

ABSTRACT

Introducción: El incremento de la multirresistencia bacteriana constituye un problema de salud pública a nivel internacional. Objetivos: Determinar la susceptibilidad antimicrobiana y los patrones de multirresistencia en cepas de Escherichia coli y Klebsiella pneumoniae aisladas de urocultivos. Métodos: Se realizó un estudio descriptivo retrospectivo en el Centro Municipal de Higiene, Epidemiología y Microbiología, municipio Güines, provincia Mayabeque, Cuba, en el periodo comprendido de enero a diciembre de 2017. El estudio incluyó 250 cepas de Escherichia coli y 62 de Klebsiella pneumoniae aisladas e identificadas de muestras de orina de pacientes con infección del tracto urinario adquirida en la comunidad. La susceptibilidad antimicrobiana fue evaluada con el método de difusión en agar empleado la técnica de Kirby Bauer. Resultados: En Escherichia coli se observó niveles de resistencia superiores al 60 por ciento a los antimicrobianos ácido nalidíxico, cefotaxima, trimetoprim - sulfametoxazol y ceftazidima. La nitrofurantoína y la amikacina presentaron 88,8 por ciento y 83,8 por ciento de efectividad, respectivamente. Se apreció en Klebsiella pneumoniae altos valores de resistencia a ceftazidima, trimetoprim - sulfametoxazol y ácido nalidíxico. Amikacina, presentó niveles de sensibilidad de un 71 por ciento. La resistencia a las cefalosporinas de tercera generación se detectó en 78 (31,2 por ciento) de Escherichia coli y 26 (41,9 por ciento) de Klebsiella pneumoniae. De los aislados de Escherichia coli 143 (57,2 por ciento) y Klebsiella pneumoniae 35 (56,4 por ciento) presentaron multidrogoresistencia. Conclusiones: Existe la circulación de cepas resistentes a cefalosporinas de tercera generación y multidrogorresistentes causantes de infecciones de las vías urinarias adquiridas en la comunidad y se informa sobre los antibióticos (nitrofurantoína y amikacina) que podrían ser utilizados para combatirlas de forma empírica en esta área geográfica(AU)


Introduction: The increase of bacterial multiresistance constitutes a public health problem at the international level. Objectives: To determine antimicrobial sensitivity and multiresistance patterns in strains of Escherichia coli and Klebsiellapneumoniae isolated from urine cultures. Methods: A retrospective, descriptive study was conducted at the Municipal Center for Hygiene, Epidemiology and Microbiology, Güines municipality, Mayabeque Province, Cuba, in the period from January to December, 2017. The study included 250 Escherichia coli and 62 Klebsiellapneumoniae strains isolated and identified from urine samples from patients with urinary tract infection acquired in the community. Antimicrobial sensitivity was evaluated with the method of diffusion in agar using Kirby Bauer´s technique. Results: In Escherichia coli, resistance levels higher than the 60% were observed in antimicrobial nalidixic acid, cefotaxime, trimethoprim-sulfamethoxazole and ceftazidime. Nitrofurantoin and amikacin presented 88.8 percent and 83.8 percent of effectiveness, respectively. High values of resistance to ceftazidime, trimethoprim-sulfamethoxazole and nalidixic acid were present in Klebsiellapneumoniae. Amikacin presented sensitivity levels of 71 percent. Resistance to third-generation cephalosporins was detected in 78 (31.2 percent) of Escherichia coli and 26 (41.9 percent) Klebsiellapneumoniae. From the Escherichia coli and Klebsiellapneumoniae isolates, 143 (57.2 percent) and 35 (56.4 percent),respectively, presented multidrug resistance. Conclusions: There is circulation of strains which are resistant to third generation cephalosporins and multidrug resistants that cause urinary tract infections acquired in the community and there are reports on antibiotics (nitrofurantoin and amikacin) that might be used to combat them empirically in this geographical area(AU)


Subject(s)
Humans , Male , Female , Klebsiella Infections/epidemiology , Drug Resistance, Microbial , Amikacin/therapeutic use , Cephalosporin Resistance , Escherichia coli Infections/epidemiology , Nitrofurantoin/therapeutic use , Epidemiology, Descriptive , Retrospective Studies
20.
Braz. j. infect. dis ; 23(1): 60-65, Jan.-Feb. 2019. tab, graf
Article in English | LILACS | ID: biblio-1039221

ABSTRACT

ABSTRACT Polymyxin B is one of the last resort option for carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infection in China. Therefore, the timing of administration of polymyxin is frequently delayed. We collected 40 cases of CRKP bloodstream infections (BSIs) treated with combinations based on polymyxin B over 30 months. The primary outcome, 30-day mortality rate, was 52.5% (21/40). Early administration of polymyxin B is meant to administer the drug within 48 h of diagnosing bacteremia. Delayed administration was considered when polymyxin B was administered after 48 h of bacteremia onset. Polymyxin B duration and total dosages were similar in the two groups (11.57 days versus 11.76 days, p = 0.919; 1306.52 mg versus 1247.06 mg, p = 0.711). Compared with delayed administration, early use of polymyxin B-based combination therapy had a significant increase in the rate of bacterial clearance (65.22% versus 29.41%, p = 0.025; OR = 0.533) and decreased 30-day mortality (39.13% versus 70.59%, p = 0.045; OR = 0.461) and overall mortality (43.48% versus 82.35%, p = 0.022; OR = 0.321).


Subject(s)
Humans , Male , Female , Middle Aged , Polymyxin B/administration & dosage , Klebsiella Infections/drug therapy , Bacteremia/drug therapy , Carbapenem-Resistant Enterobacteriaceae/drug effects , Anti-Bacterial Agents/administration & dosage , Klebsiella Infections/mortality , Microbial Sensitivity Tests , Reproducibility of Results , Retrospective Studies , Treatment Outcome , Bacteremia/mortality , Kaplan-Meier Estimate
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