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1.
Braz. j. biol ; 84: e250517, 2024. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355859

ABSTRACT

Abstract Lactobacilli are probiotics with Aflatoxin (AF) detoxification ability, found in fermented products, GIT of animals and environment. Purpose of this study was to investigate the ability of broiler isolates of Lactobacillus against Aflatoxin B1 (AFB1). For this purpose, 5 isolates of Lactobacillus from broiler gut were incubated with 100 ppb AFB1 in aqueous environment and effect of different parameters (cell fractions, time, temperature, pH) on detoxification was determined by HPLC. The ameliorative effect of Lactobacillus salivarius (LS) against AFB1 was studied in broiler. The results revealed that LS (CR. 4) showed the best results (in vitro) as compared to other isolates (L. salivarius (CR. 3, CR, 4), L. agilis (CE. 2.1, CE. 3.1) and L. crispatus (CE. 28). Cell debris of CR. 4 showed significantly higher detoxification (P<0.05). Maximum amount of AFB1 was detoxified at 30°C (97%), pH 4.0 (99%) and 6 h (99.97%). In vivo study showed that AFB1 decreased weight gain (1,269 ± 0.04 gm/ bird), feed consumed (2,161 ± 0.08 gm/ bird), serum total protein (2.42 ± 0.34 gm/ dl), serum albumin (0.5 ± 0.2 2 gm/dl) and antibody titer (4.2 ± 0.83). Liver function enzymes were found (alanine transaminase (ALT): 32 ± 10.7 U/L) and aspartate transaminase (AST): 314.8 ± 27 U/L) elevated in AFB1 fed broilers. Treatment with 1% LS not only decreased the toxic effects of AFB1 (group D) but also improved the overall health of broilers due to its probiotic effects (p<0.05) as compared to control negative (group A). The detoxification ability of LS was better than commercial binder (CB) (0.2% Protmyc). It was concluded that detoxification of AFB1 by Lactobacillus was strain, temperature, pH and time dependent. LS has detoxification ability against AFB1 in vivo.


Resumo Os lactobacilos são probióticos com capacidade de desintoxicação da Aflatoxina (AF), encontrados em produtos fermentados, TGI de animais e meio ambiente. O objetivo deste estudo foi investigar a capacidade de isolados de frango de corte de Lactobacillus contra a Aflatoxina B1 (AFB1). Para tanto, 5 isolados de Lactobacillus de intestino de frango foram incubados com 100 ppb AFB1 em meio aquoso, e o efeito de diferentes parâmetros (frações celulares, tempo, temperatura, pH) na desintoxicação foi determinado por CLAE. O efeito melhorador de Lactobacillus salivarius (LS) contra AFB1 foi estudado em frangos de corte. Os resultados revelaram que LS (CR. 4) apresentou os melhores resultados (in vitro) em comparação com outros isolados [L. salivarius (CR. 3, CR. 4), L. agilis (CE. 2.1, CE. 3.1) e L. crispatus (CE. 28)]. Detritos celulares de CR. 4 mostraram desintoxicação significativamente maior (P < 0.05). A quantidade máxima de AFB1 foi desintoxicada a 30 °C (97%), pH 4.0 (99%) e 6 h (99,97%). O estudo in vivo mostrou que AFB1 diminuiu o ganho de peso (1,269 ± 0.04 g / ave), alimento consumido (2,161 ± 0.08 g / ave), proteína total sérica (2.42 ± 0.34 g / dl), albumina sérica (0.5 ± 0.22 gm / dl) e título de anticorpo (4.2 ± 0.83). As enzimas da função hepática foram encontradas (alanina transaminase (ALT): 32 ± 10.7 U / L) e aspartato transaminase (AST): 314.8 ± 27 U / L) elevadas em AFB1 alimentados com frangos. O tratamento com 1% LS não só diminuiu os efeitos tóxicos de AFB1 (grupo D), mas também melhorou a saúde geral dos frangos devido aos seus efeitos probióticos (p < 0.05) em comparação com o controle negativo (grupo A). A capacidade de desintoxicação do LS foi melhor do que o aglutinante comercial (CB) (0.2% Protmyc). Concluiu-se que a desintoxicação de AFB1 por Lactobacillus foi dependente da cepa, temperatura, pH e tempo. LS tem capacidade de desintoxicação contra AFB1 in vivo.


Subject(s)
Animals , Aflatoxin B1/analysis , Aflatoxin B1/toxicity , Probiotics , Chickens , Lactobacillus , Animal Feed/analysis
2.
Braz. dent. sci ; 25(2): 1-9, 2022. tab
Article in English | LILACS, BBO | ID: biblio-1369265

ABSTRACT

Objetivo: existem evidências inconclusivas sobre a potencial carogenicidade das fórmulas lácteas em comparação com o leite materno. O estudo teve como objetivo comparar a detecção e contagem de Streptococcus mutans(S. mutans) e lactobacilos da saliva entre lactentes alimentados com leite materno (B), com fórmulas de leite puro (France Lait-FL) e suplementada com probióticos (Nan 1 optipro-N), e avaliar crescimento in vitro dessas bactérias em amostras de leite materno e fórmulas lácteas. Material e Métodos: amostras salivares foram obtidas com swabs de algodão estéreis de 60 lactentes que foram agrupados de acordo com o tipo de leite. As amostras foram cultivadas para obter a frequência de detecção e contagens bacterianas. Para a investigação in vitro, sete amostras de leite materno doado e sete amostras recém-preparadas de ambas as fórmulas lácteas foram inoculadas por ambas as bactérias e então cultivadas para avaliar o crescimento bacteriano. Resultados:lactobacilos foram detectados em todos os lactentes, enquanto não foram encontradas diferenças significativas na detecção de S. mutans entre os grupos. As contagens de ambos os microrganismos na saliva foram menores em (B), enquanto uma diferença insignificante foi encontrada entre (B) e (N). Diferenças significativas foram evidentes nas contagens bacterianas in vitro sendo mais baixas em (B) seguido por (N) e (FL). Conclusão: o leite materno e as fórmulas lácteas com suplementos probióticos podem ter um papel protetor contra a cárie dentária em lactentes. (AU)


Objective: inconclusive evidence exists regarding potential cariogenicity of milk formulas compared to breast milk. The study aimed to compare Salivary Streptococcus mutans (S. mutans) and lactobacilli detection and counts among breastfed (B), plain formula (France Lait 1) (FL) and probiotic supplemented formula (Nan 1 optipro) (N) infants and to assess in-vitro growth of these bacteria in breast milk and milk formula samples. Material and Methods:salivary samples were obtained using sterile cotton swabs from 60 infants that were grouped according to nursing milk type. Samples were cultured to obtain the detection frequency and bacterial counts. For the in-vitro investigation, seven donated breast milk samples and seven freshly prepared samples of both milk formulas were inoculated by both bacteria and then cultured to assess bacterial growth. Results:lactobacilli were detected in all infants, while no significant differences were found in S. mutans detection among groups. Counts of both microorganisms in saliva were lowest in (B) while, insignificant difference was found between (B) and (N). Significant differences were evident in in-vitro bacterial counts being lowest in (B) followed by (N) and (FL). Conclusion: breast milk and probiotic supplement infants' milk formulas may have a protective role against dental caries in infants.(AU)


Subject(s)
Humans , Infant , Streptococcus mutans , Probiotics , Dental Caries , Infant Formula , Lactobacillus , Milk, Human
4.
Univ. salud ; 23(2): 151-161, mayo-ago. 2021. tab, graf
Article in Spanish | LILACS, COLNAL | ID: biblio-1252319

ABSTRACT

Introducción: En producción avícola el uso de antibióticos promotores del crecimiento es limitado, debido al incremento de resistencia bacteriana. Una alternativa evalúa los probióticos microencapsulados y su efecto en la salud intestinal. Objetivo: Determinar el efecto de Lactobacillus plantarum microencapsulado sobre parámetros intestinales e inmunológicos en pollos de engorde. Materiales y métodos: A 240 pollos Ross-308-AP de un día de nacidos se suministró alimento con o sin adición de probiótico bajo el siguiente modelo: sin probiótico-(T0), con probiótico comercial-(T1), con L. plantarum microencapsulado-(T2) y sin microencapsular-(T3). L. plantarum ATCC-8014 se microencapsuló mediante secado por aspersión, determinando su viabilidad en (%). Se evaluaron parámetros intestinales, morfo-histopatológicos e inmunológicos por Azul de Alcian, microscopia de barrido e inmunohistoquímica y la abundancia microbial por UFC/mL. Resultados: El microencapsulado confirió una viabilidad in vivo de L. plantarum del 88,1%. El tratamiento T2 mejoró los parámetros inmunológicos y confirió beneficios intestinales con una abundancia de bacterias benéficas (Lactobacillus) de (9,13x105-UFC/mL), significativamente mayor a la encontrada en los tratamientos T1 (8,91x105) y T3 (8,23x105) y el control T0 (9,18x104), (p<0,05). Conclusiones: La adición de L. plantarum microencapsulado en alimento para pollos mejora parámetros inmunológicos y confiere mayor abundancia de bacterias benéficas presentes en la microbiota intestinal.


Introduction: Usage of growth-promoting antibiotics in poultry production is limited due to the increase in bacterial resistance. An alternative to assess microencapsulated probiotics and their effect on gut health is presented in this study. Objective: To determine the effect of microencapsulated L. plantarum on intestinal and immunological parameters in broilers. Materials and methods: 240 Ross-308-AP chickens (one day old) were fed with or without the addition of a probiotic, under the following model: without probiotic (T0); with commercial probiotic (T1); with probiotic containing either microencapsulated (T2) or non-microencapsulated (T3) L. plantarum. ATCC-8014 was microencapsulated by spray drying, assessing its viability in (%). Alcian blue, scanning microscopy, and immunohistochemistry were used to evaluate intestinal, morpho-histopathological, and immunological parameters. Microbial abundance was quantified by UFC/ml. Results: Microencapsulation of L. plantarum induced an 88.1% in vivo viability. T2 treatment improved both immunological parameters and the intestinal population of beneficial bacteria (Lactobacillus) (9.13x105 UFC/ml), which was significantly higher than that found in T1 (8.91x105), T3 (8.23x105), and control T0 (9.18x104), (p<0.05). Conclusion: Adding microencapsulated L. plantarum to chicken feed improves immunological parameters and increases the population of beneficial bacteria in the intestinal microbiota.


Subject(s)
Functional Food , Lactobacillus , Bacteria , Probiotics , Microbiota
5.
Article in Chinese | WPRIM | ID: wpr-888084

ABSTRACT

Bupleuri Radix, serving as the sovereign medicinal in many antidepressant compound preparations, has been proved effective in treating depression in mice, but its effect on the intestinal flora remains unclear. The present study aimed to investigate the effects of Bupleurum chinense(one of the original materials of Bupleuri Radix) on the behaviors and the diversity of intestinal flora of depressed mice. A depression mouse model was induced by repeated social defeat stress. Specifically, C57 BL/6 J male mice were exposed to the attack from the CD-1 mice. Then, C57 BL/6 J male mice were divided into a depression group and a B. chinense group, with normal saline and B. chinense administered(ig) respectively. Sucrose preference test and tail suspension test were conducted during and after the experiment respectively, to analyze the effects of B. chinense on the behaviors of the depressed mice. The feces were collected after the experiment. The V3-V4 16 S rDNA regions of intestinal flora of mice in each group were sequenced by Ion S5 TMXL for the analysis of the number of operational taxonomic units(OTUs), richness, alpha and beta diversity indexes, and differential phyla and genera. The results indicated that B. chinense could decrease depressive-like behaviors of mice, increase sucrose preference, and shorten the time of immobility in tail suspension test. After B. chinense intervention, the relative abundance of Firmicutes was significantly decreased, while that of Bacteroidetes was increased at the phylum level. At the genus level, the relative abundance of Lactobacillus and Lachnoclostridium decreased(P<0.05), while that of Bacteroides, Alistopes, etc. was elevated(P<0.05). The findings demonstrate that B. chinense can regulate the intestinal flora and improve the depressive-like behaviors of mice with depression.


Subject(s)
Animals , Bupleurum , Feces , Gastrointestinal Microbiome , Lactobacillus , Mice , Mice, Inbred C57BL
6.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2381-2386, Nov.-Dec. 2020. graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1142329

ABSTRACT

A produção aquícola mundial tem demandado estudos que buscam soluções para os problemas produtivos e sanitários apresentados na piscicultura. Nesse contexto, o uso de produtos probióticos demonstra ser uma abordagem promissora. Dessa forma, o presente trabalho teve como objetivo avaliar a viabilidade de um pool de cepas de Lactobacillus incorporados à ração de truta-arco-íris. Para tanto, as respectivas cepas foram previamente cultivadas em caldo MRS e submetidas à criocentrifugação. As células obtidas foram ressuspensas em soro fisiológico acrescentado de 2% de óleo de soja, sendo posteriormente incorporadas à ração por meio de misturador circular. Essa ração foi devidamente armazenada sob refrigeração a 4°C, por 120 dias, sendo retiradas aleatoriamente três amostras, a cada 15 dias, para avaliação da viabilidade das bactérias, por meio da contagem em placas. Os resultados demonstraram que houve uma redução não significativa (P>0,05) na população de células viáveis, correspondente a 2%, após 30 dias de armazenamento. Verificou-se também, após esse período, uma população aproximada equivalente a 3,00 x 108 UFC/g. Dessa forma, demonstrou-se a possibilidade de desenvolvimento de formulações probióticas por meio da incorporação de cepas específicas em rações de trutas, o que poderá contribuir para o desenvolvimento de tecnologias alternativas para produção de peixes.(AU)


Among the exotic species of fish produced in Brazil, the rainbow trout (Oncorhynchus mykiss) has been highlighted during the past few years. The expansion of this sector has been intensifying the need for further studies in order to decrease production and health problems presented in aquaculture, as well as to develop alternative technics to controversial antimicrobial treatments. In this context, the use of probiotics has been shown to be a promising approach to the improvement in trout husbandry. Thus, the present study aimed to contribute to the development of an alternative technology in the rainbow trout husbandry by evaluating the effect of probiotic preparations on the performance of the animals subjected to conventional husbandry conditions or chronic stress. Therefore, in this paper we report the results regarding the evaluation of the viability of a pool of 4 Lactobacillus probiotic strains incorporated into rainbow trout ration using soybean oil as vehicle. The results have demonstrated a high efficacy of cell incorporation into the ration with high viability rate after storage for 120 days at 4°C.(AU)


Subject(s)
Animals , Oncorhynchus mykiss/growth & development , Probiotics/administration & dosage , Lactobacillus , Animal Feed/analysis
7.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 871-878, May-June, 2020. tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1129543

ABSTRACT

The objective of this study was to evaluate the hematological and immunological parameters of yellowtail lambaris (Astyanax bimaculatus), fed with different frequencies of the probiotic (Lactobacillus spp.). Fishes were distributed into 20 experimental units and divided in five treatments: control (0%), 25%, 50%, 75% and 100% of probiotic supply. A higher presence of total leukocytes (47.70 103cell µl-1), lymphocytes (36.11 103cell µl-1) and monocytes (11.58 103cell µl-1) was verified in fish fed 100% of probiotic, showing a directly proportional ratio among the frequencies of the probiotic supply on the availability of circulating cells in the circulatory system (R² 094-0.97). Hematocrit (27.30-34.63%), hemoglobin (7.00-10.90g dl-1), mean corpuscular volume (4.21-5.45 10-5.pg), mean corpuscular hemoglobin (3.45-5.40 10-6.pg), mean corpuscular hemoglobin concentration, (2.99-4.35g dl-1), total protein (44.32-50.26mg ml-1) and total plasma immunoglobulin (27.96-34.08mg ml-1) did not diverge among treatments. The frequency of the probiotic supply interferes with the hematological profile, although lactic acid bacteria were present in the same concentrations in the intestinal tract, regardless of the probiotic supply, there was an increase in circulating leukocytes, especially lymphocytes and monocytes, in lambari fed probiotic with more frequency.(AU)


O objetivo do presente trabalho foi avaliar os parâmetros hematológicos e imunológicos do lambari-do-rabo-amarelo (Astyanax bimaculatus) alimentado com diferentes frequências de probiótico (Lactobacillus spp.). Os peixes foram distribuídos em 20 unidades experimentais e divididos em cinco tratamentos: controle (0%), 25%, 50%, 75% e 100% de frequência na suplementação probiótica. Alta presença de leucócitos totais (47,70 10³ células µ-1), linfócitos (36,11 10³ células µ-1) e monócitos (11,58 10³ células µ-1) em peixes alimentados com 100% de probiótico apresenta uma taxa diretamente proporcional entre as frequências da suplementação probiótica na disponibilidade das células no sistema circulatório (R² 094-0,97). Hematócrito (27,30-34,63%), hemoglobina (7,00-10,90g dL-¹), volume corpuscular médio (4,21-5,45 10-5.pg), hemoglobina corpuscular média (3,45-5,40 10-6.pg), concentração de hemoglobina corpuscular média (2,99-4,35g dl-1), proteína total (44,32-50,26mg ml-1) e imunoglobulina plasmática total (27,96-34,08mg ml-1) não divergiram entre os tratamentos. A frequência da suplementação probiótica interferiu no perfil hematológico. Embora as bactérias ácido láticas estejam presentes na mesma concentração no trato intestinal, independentemente da oferta de probiótico, houve um aumento na circulação de leucócitos, especialmente linfócitos e monócitos, nos lambaris alimentados com maior frequência.(AU)


Subject(s)
Animals , Probiotics/administration & dosage , Characiformes/blood , Lactobacillus , Lactic Acid
8.
Electron. j. biotechnol ; 43: 16-22, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1087512

ABSTRACT

Background: The intestinal bacterial community has an important role in maintaining human health. Dysbiosis is a key inducer of many chronic diseases including obesity and diabetes. Kunming mice are frequently used as a model of human disease and yet little is known about the bacterial microbiome resident to the gastrointestinal tract. Results: We undertook metagenomic sequencing of the luminal contents of the stomach, duodenum, jejunum, ileum, cecum, colon, and rectum of Kunming mice. Firmicutes was the dominant bacterial phylum of each intestinal tract and Lactobacillus the dominant genus. However, the bacterial composition differed among the seven intestinal tracts of Kunming mice. Compared with the small intestine, the large intestine bacterial community of Kunming mice is more stable and diverse. Conclusions: To our knowledge, ours is the first study to systematically describe the gastrointestinal bacterial composition of Kunming mice. Our findings provide a better understanding of the bacterial composition of Kunming mice and serves as a foundation for the study of precision medicine.


Subject(s)
Animals , Mice , Bacteria/isolation & purification , Gastrointestinal Tract/microbiology , Bacteria/genetics , RNA, Ribosomal, 16S , Polymerase Chain Reaction , High-Throughput Nucleotide Sequencing , Firmicutes/isolation & purification , Gastrointestinal Microbiome , Lactobacillus/isolation & purification
9.
Electron. j. biotechnol ; 43: 23-31, Jan. 2020. ilus, graf
Article in English | LILACS | ID: biblio-1087514

ABSTRACT

Background: Hong Qu glutinous rice wine (HQGRW) is brewed under non-aseptic fermentation conditions, so it usually has a relatively high total acid content. The aim of this study was to investigate the dynamics of the bacterial communities and total acid during the fermentation of HQGRW and elucidate the correlation between total acid and bacterial communities. Results: The results showed that the period of rapid acid increase during fermentation occurred at the early stage of fermentation. There was a negative response between total acid increase and the rate of increase in alcohol during the early fermentation stage. Bacterial community analysis using high-throughput sequencing technology was found that the dominant bacterial communities changed during the traditional fermentation of HQGRW. Both principal component analysis (PCA) and hierarchical clustering analysis revealed that there was a great difference between the bacterial communities of Hong Qu starter and those identified during the fermentation process. Furthermore, the key bacteria likely to be associated with total acid were identified by Spearman's correlation analysis. Lactobacillus, unclassified Lactobacillaceae, and Pediococcus were found, which can make significant contributions to the total acid development (| r| N 0.6 with FDR adjusted P b 0.05), establishing that these bacteria can associate closely with the total acid of rice wine. Conclusions: This was the first study to investigate the correlation between bacterial communities and total acid during the fermentation of HQGRW. These findings may be helpful in the development of a set of fermentation techniques for controlling total acid.


Subject(s)
Bacteria/isolation & purification , Wine/microbiology , Pediococcus/isolation & purification , Pediococcus/genetics , Pediococcus/metabolism , Time Factors , Acetobacter/isolation & purification , Acetobacter/genetics , Acetobacter/metabolism , Cluster Analysis , Sequence Analysis , Computational Biology , Principal Component Analysis , Fermentation , Microbiota , Hydrogen-Ion Concentration , Lactobacillus/isolation & purification , Lactobacillus/genetics , Lactobacillus/metabolism
11.
Mem. Inst. Oswaldo Cruz ; 115: e200377, 2020. tab, graf
Article in English | SES-SP, LILACS, SES-SP | ID: biblio-1143303

ABSTRACT

BACKGROUND Visceral leishmaniasis (VL) is a tropical neglected disease with high associated rates of mortality. Several studies have highlighted the importance of the intestinal tract (IT) and gut microbiota (GM) in the host immunological defense. Data in the literature on parasite life cycle and host immune defense against VL are scarce regarding the effects of infection on the IT and GM. OBJECTIVES This study aimed to investigate changes observed in the colon of Leishmania infantum-infected hamsters, including alterations in the enteric nervous system (ENS) and GM (specifically, levels of bifidobacteria and lactobacilli). METHODS Male hamsters were inoculated with L. infantum and euthanised at four or eight months post-infection. Intestines were processed for histological analysis and GM analysis. Quantitative polymerase chain reaction (qPCR) was performed to quantify each group of bacteria: Bifidobacterium spp. (Bf) and Lactobacillus spp (LacB). FINDINGS Infected hamsters showed histoarchitectural loss in the colon wall, with increased thickness in the submucosa and the mucosa layer, as well as greater numbers of intraepithelial lymphocytes. Forms suggestive of amastigotes were seen inside mononuclear cells. L. infantum infection induced changes in ENS, as evidenced by increases in the area of colonic enteric ganglia. Despite the absence of changes in the levels of Bf and LacB during the course of infection, the relative abundance of these bacteria was associated with parasite load and histological alterations. MAIN CONCLUSIONS Our results indicate that L. infantum infection leads to important changes in the colon and suggest that bacteria in the GM play a protective role.


Subject(s)
Animals , Bifidobacterium , Leishmania infantum , Gastrointestinal Microbiome , Lactobacillus , Leishmaniasis, Visceral , Cricetinae , Parasite Load , Intestines/parasitology
12.
Rev. odontopediatr. latinoam ; 10(1): 13-23, 2020. graf, ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1147434

ABSTRACT

La caries es considerada todavía una de las enfermedades más prevalentes del mundo. Por este motivo, diversas estrategias de prevención se han desarrollado en los últimos años con el propósito de controlar esta enfermedad. Diferentes especies bacterianas de la microbiota normal de la cavidad oral producen sustancias antagónicas antibacterianas. Objetivo: El objetivo de este trabajo fue identificar alguna especie del género Lactobacillus productor de sustancias antagónicas contra Streptococcus mutans a partir de saliva de niños con y sin caries. Materiales y métodos: Se aislaron desde saliva de 60 niños con caries, sin caries activa (rehabilitados) y libres de caries, diferentes cepas de Lactobacillus a las cuales se les estudió su capacidad antagónica contra cepas de Streptococcus mutans , mediante ensayos en doble capa, test del pocillo y sobre crecimiento bacteriano. Resultados: Las cepas que elaboran sustancias con mayor capacidad antagónica fueron identificadas como Lactobacillus fermentum mediante Api test 50 CH. Conclusión: Se demostró que Lactobacillus fermentum está presente en mayor porcentaje en el grupo de niños sin caries, lo cual podría sugerir un efecto natural de control biológico en la cavidad oral de este grupo de niños.


A cárie dentária ainda é considerada uma das doenças mais prevalentes no mundo. Por esta razão, várias estratégias de prevenção foram desenvolvidas nos últimos anos com o objetivo de controlar esta doença. Diferentes espécies bacterianas da microbiota normal da cavidade oral produzem substâncias antagonistas antibacterianas. Objetivo:O objetivo deste estudo foi identificar algumas espécies do gênero Lactobacillus produtoras de substâncias antagonistas contra Streptococcus mutans da saliva de crianças com e sem cárie. Materiais e Métodos: Foram isolados a partir da saliva de 60 crianças com cavidades, sem cavidades ativas (restaurada) e livre de lesões de cárie, diferentes tipos de Lactobacillus que foram estudados pela sua capacidade antagonista contra Streptococcus mutans, por testes camada dupla e bem sobre o crescimento bacteriano. Resultados: As cepas que elaboraram substâncias com maior capacidade antagônica foram identificadas como Lactobacillus fermentum pelo teste Api 50 CH. Conclusões: Foi demonstrado que o Lactobacillus fermentum está presente em maior porcentagem no grupo de crianças sem cárie, o que poderia sugerir um efeito natural do controle biológico na cavidade bucal desse grupo de crianças


Caries is still considered one of the most prevalent diseases worldwide. For this reason, in recent years various prevention strategies have been developed with the purpose of managing this disease. Different oral cavity bacterial species part of the normal microbiota produce antibacterial antagonistic substances. Objective: The objective of this study was to identify some species of Lactobacillus genus that produce antagonistic substances against Streptococcus mutans from saliva of children with and without caries. Materials and methods: Different Lactobacillus strains were isolated from saliva of 60 children with caries, without active decay (rehabilitated) and free of caries. The antagonistic capacity against strains of Streptococcus mutans was studied, by means of tests in double layer, test of the well and about bacterial growth Results: Lactobacillus strains that produced substances with greater antagonistic capacity were identified as Lactobacillus fermentum by Api test 50 CH. Conclusions: It was found that Lactobacillus fermentum is present in a larger percentage among children without caries which could suggest a natural biological control effect by this bacterial strain.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Streptococcus mutans , Dental Caries , Lactobacillus , Wounds and Injuries , Bacterial Growth , Disease Prevention , Microbiota , Methods
13.
Rev. bras. anal. clin ; 51(3): 219-229, 20190930. ilus, tab
Article in Portuguese | LILACS | ID: biblio-1047645

ABSTRACT

Objetivo: Identificar alterações reativas celulares predominantes frente ao infiltrado inflamatório e ao morfotipo lactobacilar vaginal. Métodos: Estudo descritivo qualitativo, calculado pelo Qui-quadrado com 198 amostras. Realizados esfregaços de raspado cervicovaginal pelo Papanicolaou e secreção de fundo de saco vaginal para pesquisa de microbiota vaginal pelo Gram. Resultados: Forma curta de lactobacilos em 186 (93,9%) amostras e longos em 12 (6,1%). Infiltrado inflamatório pelo Gram escasso em 90 (45,45%) e Papanicolaou escassos em 82 (41,04%). Fagocitose pelo Gram escasso em 51 (25,76%). Alterações reativas celulares pelo Papanicolaou: citólise escassa (23,74%), edema nuclear moderado (35,86%), cariomegalia escassa (13,64%), binucleação escassa (35,35%), micronúcleo escasso (2,52%) e paraqueratose moderada em (23,74%). Qui-quadrado significativo entre a quantificação de infiltrado inflamatório pelo Gram e Papanicolaou com p=0,037. 30 (15%) enquadraram-se no diagnóstico laboratorial de vaginose citolítica. Critérios não clássicos e de micronúcleo e paraqueratose presentes em amostras com dois critérios simultaneamente em 25%, três simultâneos em 4,0% e quatro simultâneos em 2,0%. Conclusão: Critérios não clássicos podem aumentar a sensibilidade dos testes citológicos no advento da citologia reflexiva nos testes de HPV.


Objective: Identify predominant cells reactive changes against the inflammatory infiltrate and the lactobacillary vaginal morphotype. Methods: Qualitative descriptive study, calculated by chi-square with Alterações celulares reativas frente ao morfotipo de lactobacilos vaginais 228 RBAC. 2019;51(3):219-29 Neves JJ, Reche PM, Ravelli APX, Ito CAS, Fagundes GL, Machado EP 198 samples. Cervicovaginal scrapings were realized by the Papanicolaou, and vaginal pouch fundus secretion to investigate the vaginal microbiota by Gram. Results: Short form of lactobacilli in 186 (93.9%) samples and long in 12 (6.1%). Inflammatory infiltrate by Gram, scarce in 90 (45.45%) and Papanicolaou in 82 (41.04%). Fagocytosis by Gram, scarce in 51 (25.76%). Papanicolaou cells reactive changes: scarce cytolysis (23.74%), moderate nuclear swelling (35.86%), scarce caryomegaly (13.64%), scarce binucleation (35.35%), scarce micronucleus (2.52% ) and moderate parakeratosis (23.74%). Significant chi-square between the quantification of inflammatory infiltrate by Gram and Papanicolaou with p = 0.037. 30 (15%) were in the laboratory diagnosis of CV. Non-classical criteria, and micronucleus and parakeratosis present in samples with two criteria simultaneously in 25%, three simultaneous in 4.0% and four simultaneous in 2.0%. Conclusion: Non-classical criteria may increase the sensitivity of cytological tests in the advent of refletive cytology in the HPV tests.


Subject(s)
Humans , Female , Adolescent , Adult , Middle Aged , Aged , Uterine Cervical Neoplasms/prevention & control , Papanicolaou Test , Microbiota , Lactobacillus
15.
Arq. bras. med. vet. zootec. (Online) ; 71(2): 647-657, mar.-abr. 2019. tab
Article in English | LILACS, VETINDEX | ID: biblio-1011286

ABSTRACT

Minas artisanal cheese is made from endogenous starter cultures, including lactic acid bacteria (LAB). Some LAB may possess probiotic potential. Thus, this study aimed to evaluate the in vitro probiotic properties of lactobacilli isolated from Minas artisanal cheeses produced in Minas Gerais. Ten samples of lactobacilli, formerly isolated from those cheeses, were submitted to the following assays: antimicrobial susceptibility, tolerance to artificial gastric juice and biliary salts, production of hydrogen peroxide and antagonism against pathogenic and non-pathogenic micro-organisms. Only L. plantarum (C0) was sensitive to all tested antimicrobials, while the other LAB samples were resistant to at least one drug. Six samples were tolerant to artificial gastric juice, and L. brevis (A6) even grew in that medium. Three samples were tolerant to biliary salts. Only L. brevis (E35) produced hydrogen peroxide. Difference (P< 0.05) was observed among the means of inhibition haloes of lactobacilli against Enterococcus faecalis ATCC 19433 and Lactobacillus plantarum C24 in spot-on-the-lawn assay. All samples of lactobacilli inhibited Escherichia coli ATCC 25922, Salmonella enterica var. Typhimurium ATCC 14028 in co-culture antagonism test (P< 0.0001). Most lactobacilli samples showed in vitro probiotic potential. From the tested samples, L. brevis (A6) presented the best results considering all in vitro probiotic tests.(AU)


O queijo minas artesanal é produzido por culturas starters endógenas, incluindo bactérias ácido-láticas (BAL). Algumas BAL podem possuir potencial probiótico. Com isso, este estudo teve como objetivo avaliar as propriedades probióticas in vitro de lactobacilos isolados de queijo minas artesanal produzido no estado de Minas Gerais. Dez amostras de lactobacilos, previamente isoladas desses queijos, foram submetidas aos seguintes testes: susceptibilidade aos antimicrobianos, tolerância ao suco gástrico artificial e aos sais biliares, produção de peróxido de hidrogênio e antagonismo contra micro-organismos patogênicos e não patogênicos. Apenas L. plantarum (C0) foi sensível a todos os antimicrobianos testados, enquanto as outras amostras de BAL foram resistentes a, pelo menos, uma droga testada. Seis amostras foram tolerantes ao suco gástrico artificial, e L. brevis (A6) apresentou crescimento nesse meio. Três amostras foram tolerantes aos sais biliares. Apenas L. brevis (E35) produziu peróxido de hidrogênio. Diferença (P<0,05) foi observada entre as médias dos halos de inibição de lactobacilos contra Enterococcus faecalis ATCC 19433 e Lactobacillus plantarum C24 no teste do spot-on-the-lawn. Todas as amostras de lactobacilos inibiram Escherichia coli ATCC 25922, Salmonella enterica var. Typhimurium ATCC 14028 no teste de antagonismo em cocultura (P<0,0001). A maioria das amostras de lactobacilos apresentou potencial probiótico in vitro. Com base nas amostras testadas, L. brevis (A6) apresentou os melhores resultados, considerando-se todos os testes probióticos in vitro.(AU)


Subject(s)
Cheese/microbiology , Probiotics/isolation & purification , Lactobacillus/isolation & purification , Antibiosis
16.
Braz. oral res. (Online) ; 33: e010, 2019. tab, graf
Article in English | LILACS | ID: biblio-989483

ABSTRACT

Abstract This in situ study aimed to evaluate the antibacterial and anti-demineralization effects of an experimental orthodontic adhesive containing triazine and niobium phosphate bioglass (TAT) around brackets bonded to enamel surfaces. Sixteen volunteers were selected to use intra-oral devices with six metallic brackets bonded to enamel blocks. The experimental orthodontic adhesives were composed by 75% BisGMA and 25% TEGDMA containing 0% TAT and 20% TAT. Transbond XT adhesive (TXT) was used as a control group. Ten volunteers, mean age of 29 years, were included in the study. The six blocks of each volunteer were detached from the appliance after 7 and 14 days to evaluate mineral loss and bacterial growth including total bacteria, total Streptococci, Streptococci mutans, and Lactobacilli. Statistical analysis was performed using GLM model - univariate analysis of variance for microhardness and 2-way ANOVA for bacterial growth (p<0.05). The 20% TAT adhesive caused no difference between distances from bracket and the sound zone at 10-µm deep after 7 and 14 days. After 14 days, higher mineral loss was shown around brackets at 10- to 30-µm deep for TXT and 0% TAT adhesives compared to 20% TAT. S. mutans growth was inhibited by 20% TAT adhesive at 14 days. Adhesive with 20% TAT showed lower S. mutans and total Streptococci growth than 0% TAT and TXT adhesives. The findings of this study show that the adhesive incorporated by triazine and niobium phosphate bioglass had an anti-demineralization effect while inhibiting S. mutans and total Streptococci growth. The use of this product may inhibit mineral loss of enamel, preventing the formation of white spot lesions.


Subject(s)
Humans , Male , Female , Adult , Young Adult , Oxides/pharmacology , Phosphates/pharmacology , Streptococcus/drug effects , Tooth Demineralization/prevention & control , Dental Cements/pharmacology , Lactobacillus/drug effects , Anti-Bacterial Agents/pharmacology , Niobium/pharmacology , Ceramics/pharmacology , Ceramics/chemistry , Double-Blind Method , Dental Cements/chemistry , Anti-Bacterial Agents/chemistry
17.
Article in English | WPRIM | ID: wpr-760202

ABSTRACT

PURPOSE: To determine whether Lactobacillus reuteri DSM 17938 plays a role in absorption of iron preparations given to children with iron deficiency anemia (IDA). METHODS: We performed a quasi-experimental study involving pre- and postintervention tests using a control group in North Sulawesi province, Indonesia, between July and September 2017. We conducted a single-blind controlled trial that included primary school children who were diagnosed with IDA based on reticulocyte hemoglobin equivalent (Ret-He) levels <27.8 pg/L. RESULTS: A total of 66 children were randomized into 2 groups. Thirty-four children received iron preparations with the addition of L. reuteri DSM 17938 (group 1), whereas the other 32 received iron preparations alone (group 2). The baseline Ret-He levels before intervention were similar in both groups. After 14 days of intervention, mean Ret-He level in group 1 changed from 24.43±1.64 to 28.21±1.72 pg/L (P=0.000). Mean Ret-He level in group 2 changed from 24.31±1.42 to 27.03±2.14 pg/L (P=0.000). Statistical analysis showed a significant increase in Ret-He levels in both groups; Ret-He levels were significantly higher in the experimental group than in the control group (P<0.05). CONCLUSION: Children with IDA receiving iron preparations with L. reuteri DSM 17938 for 14 days show higher Ret-He levels than those receiving iron preparations alone.


Subject(s)
Absorption , Anemia , Anemia, Iron-Deficiency , Child , Humans , Indonesia , Iron , Lactobacillus reuteri , Lactobacillus , Non-Randomized Controlled Trials as Topic , Reticulocytes
18.
Immune Network ; : 39-2019.
Article in English | WPRIM | ID: wpr-785822

ABSTRACT

Several gut commensals have been shown to modulate host immune response. Recently, many food derived microbes have also been reported to affect the immune system. However, a mechanism to identify immunostimulatory and immunoregulatory microbes is needed. Here, we successfully established an in vitro screening system and identified an immunoregulatory bacterium, Lactobacillus pentosus KF340 (LP340), present in various fermented foods. LP340 induced a regulatory phenotype in mice Ag presenting cells which, in turn, induced IL-10 and IFN-γ producing Type 1 regulatory T cells (Tr1 cells) from naïve CD4⁺ T cells. Naïve CD4⁺ T cells co-cultured with LP340 treated dendritic cells highly expressed cytokine receptor IL-27R and were CD49b and lymphocyte-activation gene 3 double positive. Oral administration of LP340 in mice with atopic dermatitis reduced cellular infiltration in affected ear lobes and serum IgE levels, thus, ameliorating the disease symptoms. This suggests a systemic immunoregulatory effect of LP340. These findings demonstrate that LP340, a bacterium derived from food, prevents systemic inflammation through the induction of IL-10 producing Tr1 cells.


Subject(s)
Administration, Oral , Animals , Dendritic Cells , Dermatitis, Atopic , Ear , Immune System , Immunoglobulin E , In Vitro Techniques , Inflammation , Interleukin-10 , Lactobacillus , Mass Screening , Mice , Phenotype , Receptors, Cytokine , T-Lymphocytes , T-Lymphocytes, Regulatory
19.
Article in English | WPRIM | ID: wpr-785798

ABSTRACT

Lactobacillus acidophilus UBLA-34, L. paracasei UBLPC-35, L. plantarum UBLP-40, and L. reuteri UBLRU-87 were isolated from different varieties of fermented foods. To determine the probiotic safety at the strain level, the whole genome of the respective strains was sequenced, assembled, and characterized. Both the core-genome and pan-genome phylogeny showed that L. reuteri was closest to L. plantarum than to L. acidophilus, which was closest to L. paracasei. The genomic analysis of all the strains confirmed the absence of genes encoding putative virulence factors, antibiotic resistance, and the plasmids.


Subject(s)
Drug Resistance, Microbial , Genome , Lactobacillus acidophilus , Lactobacillus , Phylogeny , Plasmids , Probiotics , Virulence Factors
20.
Article in Chinese | WPRIM | ID: wpr-774123

ABSTRACT

OBJECTIVE@#To systematically review the effect of probiotic supplementation during pregnancy and infancy in preventing atopic dermatitis in children.@*METHODS@#RevMan5.3 was used to perform a Meta analysis of randomized controlled trials on the effect of probiotic supplementation during pregnancy and infancy in preventing atopic dermatitis in children published between January 2008 and May 2018 across the world. A subgroup analysis was conducted according to the type of probiotics for intervention, follow-up time, time of probiotic supplementation, and study areas.@*RESULTS@#A total of 22 articles were selected, with 3 280 cases in the intervention group and 3 281 cases in the control group. The results of pooled effect size showed that probiotic supplementation during pregnancy and/or infancy significantly reduced the incidence rate of atopic dermatitis (RR=0.81, 95%CI: 0.70-0.93, P2 years (RR=0.74, 95%CI: 0.61-0.90, P<0.05); probiotic supplementation had a significant effect in Australia (RR=0.83, 95%CI: 0.73-0.96, P<0.05) and Europe/the United States (RR=0.74, 95%CI: 0.61-0.91, P<0.05). Heterogeneity was mainly due to follow-up time (I=62.7%) and time of probiotic supplementation (I=53.5%).@*CONCLUSIONS@#Probiotic supplementation during pregnancy and infancy helps to prevent atopic dermatitis in children, and mixed Lactobacillus-Bifidobacterium intervention has a better effect.


Subject(s)
Bifidobacterium , Child, Preschool , Dermatitis, Atopic , Female , Humans , Infant , Lactobacillus , Pregnancy , Probiotics
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