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1.
Braz. J. Pharm. Sci. (Online) ; 53(2): e16105, 2017. tab, graf
Article in English | LILACS | ID: biblio-839491

ABSTRACT

ABSTRACT When the FLT3 gene is mutated, it originates a modified receptor with structural changes, which give survival advantage and malignant hematopoietic cell proliferation. Thus, the presence of mutations in this gene is considered an unfavorable prognostic factor. A total of 85 consecutive samples of newly diagnosed untreated patients with AL were included in the study after they provided their informed consent. FLT3 gene mutations were detected by PCR. For the pediatric group, a positive correlation was observed between WBC count and the presence of FLT3-ITD in patients with AML and ALL. Furthermore, children with AML who had the FLT3-ITD mutation showed a tendency to express CD34 in blast cells. In the adult group, the AML patients with FLT3-ITD who expressed CD34 in blast cells had a tendency to worse progression. The present data indicate no association between the prognostic factors evaluated and FLT3 gene mutations in adult with AL. Yet, the presence of FLT3-ITD mutation was significantly related with WBC count in the pediatric group. These findings demonstrate that FLT3 gene mutations can be considered as independent poor prognostic factors.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Patients/statistics & numerical data , Leukemia/pathology , Adult , Genes/genetics , Mutation/genetics , Prognosis , Child , Polymerase Chain Reaction/instrumentation
2.
Rev. bras. psiquiatr ; 37(1): 49-54, Jan-Mar/2015. tab, graf
Article in English | LILACS | ID: lil-741937

ABSTRACT

Objective: Peritraumatic reactions feature prominently among the main predictors for development of posttraumatic stress disorder (PTSD). Peritraumatic tonic immobility (PTI), a less investigated but equally important type of peritraumatic response, has been recently attracting the attention of researchers and clinicians for its close association with traumatic reactions and PTSD. Our objective was to investigate the role of PTI, peritraumatic panic, and dissociation as predictors of PTSD symptoms in a cohort of police recruits (n=132). Methods: Participants were asked to complete the following questionnaires during academy training and after the first year of work: Posttraumatic Stress Disorder Checklist - Civilian Version (PCL-C), Physical Reactions Subscale (PRS), Peritraumatic Dissociative Experiences Questionnaire (PDEQ), Tonic Immobility Scale (TIS), and Critical Incident History Questionnaire. Results: Employing a zero-inflated negative binomial regression model, we found that each additional point in the TIS was associated with a 9% increment in PCL-C mean scores (RM = 1.09), whereas for PRS, the increment was 7% (RM = 1.07). As the severity of peritraumatic dissociation increased one point in the PDEQ, the chance of having at least one symptom in the PCL-C increased 22% (OR = 1.22). Conclusions: Our findings highlight the need to expand investigation on the incidence and impact of PTI on the mental health of police officers. .


Subject(s)
Animals , Humans , Mice , Chromosomal Proteins, Non-Histone/physiology , Leukemia/pathology , Myeloid-Lymphoid Leukemia Protein/genetics , Neoplastic Stem Cells/pathology , Oncogenes , Repressor Proteins/physiology , Apoptosis , Chromosomal Proteins, Non-Histone/genetics , Flow Cytometry , Leukemia/genetics , Leukemia/metabolism , Polymerase Chain Reaction , Repressor Proteins/genetics
3.
Article in Korean | WPRIM | ID: wpr-9449

ABSTRACT

PURPOSE: The purpose of this study was to identify the level of distress and posttraumatic growth in fathers of chronically ill children and also, to identify the relation between characteristics of the fathers and children and their posttraumatic growth and to investigate factors that influence posttraumatic growth. METHODS: In this study, 48 fathers who visited a university hospital in Seoul, Korea and who gave written consent completed the questionnaire between September 23 and November 19, 2013. Data were analyzed using Mann-Whitney U test, Kruskal-Wallis test, Pearson correlation coefficient and stepwise multiple regression. RESULTS: The level of distress in fathers of chronically ill children was relatively high and the majority of them were experiencing posttraumatic growth. Models including the variable (deliberate rumination, religiousness, optimism) explained 64.3% (F=26.38, p <.001) of the variance for posttraumatic growth. Deliberate rumination (beta=.59, p <.001) was the most influential factor. CONCLUSION: The findings demonstrate that it is essential for nurses to intervene and facilitate continuously so as to promote posttraumatic growth and relieve distress in fathers of chronically ill children. Furthermore, it is also necessary for nurses to find ways to develop ideal interventions to activate deliberate rumination and offer spiritual care and help maintain optimism in these individuals.


Subject(s)
Adaptation, Psychological , Adult , Child , Chronic Disease , Fathers/psychology , Female , Hospitals, University , Humans , Leukemia/pathology , Male , Neoplasms/pathology , Optimism , Regression Analysis , Social Support , Surveys and Questionnaires
4.
An. bras. dermatol ; 88(6,supl.1): 158-161, Nov-Dec/2013. graf
Article in English | LILACS | ID: lil-696794

ABSTRACT

Blastic plasmacytoid dendritic cell neoplasm is a rare and aggressive hematodermic neoplasia with frequent cutaneous involvement and leukemic dissemination. We report the case of a 76-year-old man with a 2 month history of violaceous nodules and a tumor with stony consistency, located on the head, and mandibular, cervical and supraclavicular lymphadenopathies. Multiple thoracic and abdominal adenopathies were identified on computerized tomography. Flow cytometry analysis of the skin, lymph node and bone marrow biopsies demonstrated the presence of plasmocytoid dendritic cell neoplastic precursor cells (CD4+, CD45+, CD56+ and CD123+ phenotype). After initial clinical and laboratorial complete remission with chemotherapy, the patient died due to relapse of the disease associated with the appearance of a cervical mass with medullary compromise.


A neoplasia blástica de células dendríticas plasmocitóides é uma neoplasia hematodérmica rara, agressiva, com frequente envolvimento cutâneo e disseminação leucêmica. Relatamos o caso de um homem de 76 anos com quadro clínico com 2 meses de evolução caracterizado por nódulos e tumor de tonalidade violácea, de consistência pétrea, localizados na cabeça, e linfadenopatias mandibular, cervicais e supraclaviculares. Identificaram-se múltiplas adenopatias torácicas e abdominais em tomografia computorizada. A análise por citometria de fluxo de biópsias cutânea, ganglionar e óssea demonstrou a presença de precursores neoplásicos das células dendríticas plasmocitóides (fenótipo CD4+, CD45+, CD56+ e CD123+). Após remissão clínica e laboratorial completa inicial com quimioterapia, veio a falecer por recaída da doença associada ao aparecimento de massa cervical com compromisso medular.


Subject(s)
Aged , Humans , Male , Dendritic Cells/pathology , Leukemia/pathology , Skin Neoplasms/pathology , Biopsy , Bone Marrow/pathology , Fatal Outcome , Flow Cytometry , Neoplasm Invasiveness , Skin/pathology
7.
Dermatol. argent ; 17(2): 123-127, mar.-abr.2011. ilus, tab
Article in Spanish | LILACS | ID: lil-723438

ABSTRACT

La leucemia cutis se define como la infiltración de células leucémicas a nivel de la piel. Constituye un signo de enfermedad diseminada y en ocasiones es un marcador de recidiva. Su presentación clínica es variable y comprende desde pequeñas pápulas hasta grandes nódulos o tumores. Por lo general las lesiones aparecen en forma posterior al compromiso de sangre periférica. La leucemia cutis se observa con mayor frecuencia en las leucemias monocíticas o mielomonocíticas, y su presencia implica un signo de mal pronóstico. Se presentan tres casos de pacientes con diagnóstico de leucemia mieloide aguda, síndrome mielodisplásico y leucemia mieloide crónica, que presentaron leucemias cutáneas diagnosticadas por histolopatología e inmunohistoquímica.


Subject(s)
Humans , Male , Adult , Aged , Leukemia/pathology , Skin/pathology , Leukemic Infiltration , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/complications , Leukemia, Myeloid, Acute/complications , Prognosis
8.
Rev. venez. oncol ; 22(4): 260-264, oct.-dic. 2010. ilus
Article in Spanish | LILACS | ID: lil-574575

ABSTRACT

La leucemia de células dendríticas son patologías poco frecuentes, que involucran fundamentalmente a la piel, pero con una alta tendencia de metástasis. La inmunohistoquímica es una herramienta valiosa junto con la biopsia para el diagnóstico definitivo. Se presenta el caso de una paciente joven, quien acudió por presentar una lesión en la pierna izquierda de dimensiones y características únicas, cuyo diagnóstico requirió de la utilización de marcadores monoclonales específicos en la identificación de esta entidad.


The dendritic cell leukemias are pathologies very uncommon which involve mainly the skin, but with a high tendency of metastasis. The flow cytometric is a valuable tool together with tissue biopsy for a definitive diagnosis. We present the case of a young female who complained for a left leg lesion with unique dimensions and characteristics, in where the diagnostic process required the use of the specific monoclonal markers in the identification of this particular disease.


Subject(s)
Humans , Female , Adult , Antigens, Differentiation/analysis , Dendritic Cells/physiology , Dendritic Cells/pathology , Leg Injuries , Leukemia/pathology , Leukemia/drug therapy , Lymphoma, T-Cell, Cutaneous/pathology , Tobacco Use Disorder/adverse effects , Skin Ulcer/pathology , Skin Ulcer/therapy
9.
Rev. venez. oncol ; 22(4): 222-231, oct.-dic. 2010. tab
Article in Spanish | LILACS | ID: lil-574580

ABSTRACT

El paciente con enfermedades oncológicas tiene un alto riesgo para desarrollar infecciones respiratorias, y neumonía por Pneumocystis jirovecii. En Venezuela existen pocos estudios sobre la neumocistosis en pacientes oncológicos. El objetivo de este trabajo fue detectar la presencia de Pneumocystis jirovecii en pacientes oncológicos a través de la técnica de inmunofluorescencia directa. Se recibieron, durante 10 meses, 31 muestras respiratorias (lavado broncoalveolar, esputo espontáneo e inducido, aspirados traqueales), de ellas 8 (25,5 por ciento) resultaron positivas. La distribución por tipo de cáncer fue la siguiente: 18 tumores sólidos y 13 leucemias y linfomas. La positividad entre los grupos estudiados no fue estadísticamente significativa (P>0,05). Los exámenes de laboratorio complementarios, relacionados tampoco fueron estadísticamente significativos (P>0,05). Es necesario incluir este diagnóstico en estudio microbiológico diferencial de infecciones del tracto respiratorio inferior en pacientes con cáncer, estos pacientes cursan con una sintomatología general inespecífica y tendrán una alta posibilidad de desarrollar neumocistosis.


The patient with malignancy disease has a high risk to develop respiratory infections for Pneumocystis jirovecii pneumonia. Investigations about pneumocystosis in oncological patients in Venezuela are scarce. The objective of this work was to detect Pneumocystis jirovecii in oncological patients by the method of direct immunofluorescence technique. Thirty one respiratory specimens (bronchoalveolar lavage, spontaneous and induced sputum, and tracheal aspirates) received in 10 months, 8 specimens of them (25.5) were positive the distribution by malignancy disease was the following: 18 solid tumors, and 13 leukemias, and lymphomas. No statistically significant differences were found between the studied groups and positive results (P>0.05). The complementary laboratory tests, related to the presence of Pneumocystis, were not statistically significant either P>0.05). Is necessary to include this diagnosis in the microbiological differential study of low respiratory tract infections in oncological patients, since these patients show unspecific symptoms, and have a high possibility to develop pneumocystosis.


Subject(s)
Humans , Male , Female , Middle Aged , Leukemia/pathology , Lymphoma/pathology , Pneumonia, Pneumocystis/diagnosis , Pneumonia, Pneumocystis/etiology , Pneumonia, Pneumocystis/pathology , Respiratory System/pathology , Fluorescent Antibody Technique, Direct/methods , Sputum/virology , Bacterial Infections/complications , Bronchoalveolar Lavage/methods
10.
Article in English | WPRIM | ID: wpr-127078

ABSTRACT

OBJECTIVE: To investigate the significance of the dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) parameters of diffuse spinal bone marrow infiltration in patients with hematological malignancies. MATERIALS AND METHODS: Dynamic gadolinium-enhanced MR imaging of the lumbar spine was performed in 26 patients with histologically proven diffuse bone marrow infiltration, including multiple myeloma (n = 6), acute lymphoblastic leukemia (n = 6), acute myeloid leukemia (n = 5), chronic myeloid leukemia (n = 7), and non-Hodgkin lymphoma (n = 2). Twenty subjects whose spinal MRI was normal, made up the control group. Peak enhancement percentage (Emax), enhancement slope (ES), and time to peak (TTP) were determined from a time-intensity curve (TIC) of lumbar vertebral bone marrow. A comparison between baseline and follow-up MR images and its histological correlation were evaluated in 10 patients. The infiltration grade of hematopoietic marrow with plasma cells was evaluated by a histological assessment of bone marrow. RESULTS: Differences in Emax, ES, and TTP values between the control group and the patients with diffuse bone marrow infiltration were significant (t = -11.51, -9.81 and 3.91, respectively, p 0.05). A positive correlation was found between Emax, ES values and the histological grade of bone marrow infiltration (r = 0.86 and 0.84 respectively, p < 0.01). A negative correlation was found between the TTP values and bone marrow infiltration histological grade (r = -0.54, p < 0.01). A decrease in the Emax and ES values was observed with increased TTP values after treatment in all of the 10 patients who responded to treatment (t = -7.92, -4.55, and 5.12, respectively, p < 0.01). CONCLUSION: DCE-MRI of spine can be a useful tool in detecting diffuse marrow infiltration of hematological malignancies, while its parameters including Emax, ES, and TTP can reflect the malignancies' histological grade.


Subject(s)
Adolescent , Adult , Aged , Bone Marrow Neoplasms/pathology , Child , Contrast Media , Female , Gadolinium DTPA , Hematologic Neoplasms/pathology , Humans , Image Enhancement/methods , Leukemia/pathology , Lymphoproliferative Disorders/pathology , Magnetic Resonance Imaging/methods , Male , Middle Aged , Observer Variation , Prospective Studies , Spinal Neoplasms/pathology , Young Adult
11.
Pakistan Journal of Medical Sciences. 2008; 24 (3): 468-470
in English | IMEMR | ID: emr-89557

ABSTRACT

In patients with known extramammary malignancies, Metastatic disease should be considered in the differential diagnosis of a palpable breast mass, particularly if there is a history of extramammary malignancy. Breast metastasis is usually indicative of diffuse metastatic disease and a poor prognosis. Biopsy and careful review of previous pathologic material assures prompt treatment and avoids an unnecessary radical operation. Breast metastases from extramammary tumors are rare with few cases reported. Four cases of metastasis to the breast are presented and the diagnostic problems of this condition are reviewed. Correlation between the histology of primary tumor and the cytology of breast metastatic tumors can avoid the surgical breast biopsy and unnecessary mastectomy. In conclusion, Metastasis to the breast has poor prognosis. We report a case of metastasis leukemia to breast presenting as a breast lump


Subject(s)
Humans , Female , Leukemia/pathology , Mammography , Leukemia, Myeloid/pathology
12.
Article in English | IMSEAR | ID: sea-39931

ABSTRACT

Natural killer cell malignancy is a rare and aggressive lymphoid neoplasm encompassing extra-nodal NK/T-cell lymphoma, nasal-type (ENKLN) and aggressive NK-cell lymphoma/leukemia (ANKL). A case of cutaneous ENKLN and a case of ANKL in Thai patients are reported Both patients developed hemophagocytic syndrome and shortly succumbed to death. The cells in cutaneous ENKLN are small to medium in size with minimal cytoplasm, round nuclei, irregular nuclear membrane, andfine chromatin with inconspicuous nucleoli. While that of ANKL are medium to large-sized mononuclear cells with moderate cytoplasm. Their nuclei are elongated to embryo-like with irregularly thickened nuclear membrane, fine chromatin, and small to occasional prominent nucleolus. Ancillary techniques studied on paraffin embedded tissues of both cases demonstrated that the neoplastic cells exhibit cytoplasmic CD3+, CD56+ and cytotoxic granules + by immunohistochemistry, absence of T cell receptor gene rearrangement by PCR, and presence of Epstein-Barr virus mRNA (EBER) transcripts by in situ hybridization. The authors reviewed the literature on natural killer cell neoplasm and compared the clinical characteristics, natural history, and association of Epstein-Barr virus infection with hemophagocytic syndrome.


Subject(s)
Adult , Epstein-Barr Virus Infections/physiopathology , Female , Humans , Killer Cells, Natural/pathology , Leukemia/pathology , Lymphohistiocytosis, Hemophagocytic/physiopathology , Lymphoma/pathology , Risk Factors
13.
Dermatol. argent ; 12(4): 272-275, dic. 2006.
Article in Spanish | LILACS | ID: lil-558679

ABSTRACT

Se presenta un caso de leucemia cutis en una mujer de 18 años, sin diagnóstico previo de leucemia. Consulta por nódulos y pápulas eritemato-pruriginosas. Los estudios histopatológicos y de inmunohistoquímica confirman el diagnóstico. La Paciente recibió tratamiento quimioterápico con remisión de la enfermedad hematológica y desaparición de las lesiones cutáneas sin cicatrices; posteriormente se realizó transplante de médula ósea. Se destaca la buena evolución a cinco años del diagnóstico y se realiza una revisión de la literatura.


Subject(s)
Humans , Adolescent , Female , Leukemia/diagnosis , Leukemia/pathology , Skin/pathology , Immunohistochemistry
14.
Rev. méd. Chile ; 133(4): 457-460, abr. 2005. ilus
Article in Spanish | LILACS | ID: lil-417385

ABSTRACT

Natural killer leukemia is a rare and highly aggressive neoplasm, is more common in young male patients and has a very poor prognosis, with a median survival of few weeks. We report a 17 years old male patient who developed, after an acute upper respiratory disease, a rapidly multiorganic failure with pancytopenia. Bone marrow aspiration and trephine biopsy showed an acute lymphoblastic leukemia. The immunophenotype and immunohistochemistry revealed a natural killer acute leukemia. The disease progressed rapidly and the patient died shortly after the diagnosis.


Subject(s)
Adolescent , Killer Cells, Natural , Leukemia/immunology , Leukemia/pathology , Leukemia/therapy , Antigens, CD/immunology , Biopsy
15.
Medical Journal of Mashad University of Medical Sciences. 2005; 48 (89): 237-246
in Persian | IMEMR | ID: emr-73296

ABSTRACT

The term" small round cell tumour"describes a group of highly aggressive tumours composed of relatively small and monotonous undifferentiated cells with high nuclear to cytoplasmic ratio. This group includes: Ewing sarcoma [EWS], Peripheral neuroepithelioma [PN], Primitive neuroectodermal tumour [PNET], Neuroblastoma, Rhabdomyosarcoma [RMS], Desmoplastic small round cell tumour [DSRCT], Lymphoma, Leukemia, Small cell osteosarcoma Small cell carcinoma, Olfactory neuroblastoma, Merkel cell carcinoma, Small cell melanoma, and Mesenchymal chondrosarcoma. Their clinical presentation often overlap, thus making a definitive diagnosis problematic in some cases. Yet, a clear undrestanding of their clinicopathologic features usually allows for a confident diagnosis, especially if immunohistochemistry is used. This is an immunohistochemistry study of small round cell tumours with unknown origin that were diagnosed in pathology service of Imam Reza Hospital from 1362-1382. In this period, we found 24 cases which were reported SRCT without definitive diagnosis the immunohistochemistry kits are from DAKO Company and applied as directed by manufacturers. The SRCT is most frequent in the first decade of life [45%], 13 cases were male and 11 cases were female. IHC influence on diagnosis in 11 cases [46%] confirm the first ordered diagnosis and diagnosis made in another 9 [33%] of SRCTs,and in 2cases[8%]the first diagnosis was changed. Overally in review, 35 cases from 48 SRCTs with unknown origin [%75] were diagnosed by routine stainings by light microscopy without immunohistochemistry


Subject(s)
Humans , Male , Female , Carcinoma, Small Cell/diagnosis , Stem Cells , Sarcoma, Ewing/pathology , Neuroectodermal Tumors, Primitive, Peripheral/pathology , Neuroblastoma/pathology , Rhabdomyosarcoma/pathology , Lymphoma/pathology , Leukemia/pathology , Sarcoma, Small Cell/pathology , Immunohistochemistry
16.
Salvador; s.n; 2005. 73 p. ilus.
Thesis in Portuguese | LILACS | ID: biblio-1001028

ABSTRACT

As leucemias são neoplasias hematológicas decorrentes do desequilíbrio entre as taxas de proliferação, maturação e apoptose das células hematopoéticas, comumente associadas com polimorfismos gênicos. Polimorfismos nos genes do TNFa e da enzima MTHFR têm sido associados como fatores de susceptibilidade para diversas patologias, inclusive neoplasias hematológicas. O presente estudo investigou a freqüência do polimorfismo -308 da região promotora no gene do TNFa, cuja variante genotipica AA está associada a níveis de transcrição elevados da citocina, e dos polimorfismos C677T e A1298C no gene da MTHFR, que estão associados com atividade enzimática reduzida. Para investigação dos polimorfismos foi utilizada a técnica de PCR-RFLP. Foram estudados 94 pacientes leucêmicos, sendo 66 portadores de LMC e 28 de LMA-M3, além de 100 indivíduos da população de Salvador. Dos 66 pacientes com LMC, cinco (7,6%) foram homozigotos para a variante genotipica menos comum (AA) do polimorfismo -308 do TNF. Essa freqüência foi de 3,7% entre os 28 portadores de LMA-M3 e de 3,0% entre os indivíduos do grupo populacional. Dentre os portadores de 66 LMC, dois (3,0%) foram homozigotos para a variante TT do polimorfismo C677T da MTHFR, sendo que foi encontrada frequência de 7,1% entre os portadores de LMA- M3 e de 6,0% para o grupo populacional. Para o polimorfismo A1298C da MTHFR, as freqüências encontradas para a variante CC foram de 4,5% entre os 66 portadores de LMC, 3,6% entre os 28 portadores de LMA-M3 e 5,0% entre os indivíduos do grupo populacional. Diante dos resultados obtidos, concluímos que os polimorfismos -308 (TNFa), bem como C677T e A1298 (MTHFR) parecem não interferir diretamente com os mecanismos de patogênese da LMC e LMA-M3.


Subject(s)
Humans , Genes/genetics , Genes/immunology , Leukemia/immunology , Leukemia/mortality , Leukemia/pathology , Leukemia/prevention & control , Leukemia/blood , Leukemia/drug therapy
17.
Indian J Pathol Microbiol ; 2004 Jul; 47(3): 351-3
Article in English | IMSEAR | ID: sea-75717

ABSTRACT

Myxomatous stromal changes and bone marrow necrosis (BMN) are uncommon histologic findings. These changes have been found in various conditions like disseminated carcinomatosis, postchemotherapy cases, chronic infections, infiltrative disorders of the marrow etc. The present study is a retrospective study of 3 years (Jan, 1999 to Dec. 2001) from Deptt. Of Hematology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh (India). During this period, 3740 bone marrow samples were examined. Myxomatous stromal changes and bone marrow necrosis were noted in 0.43% (16/3740) and 0.45% (17/3740) samples respectively. In addition to common causes of myxomatous stromal changes and bone marrow necrosis as described in the literature, this study highlights the association of these conditions with some of the rarer entities like hyperoxalosis, leishmaniasis, parvovirus induced marrow aplasia and cryptococcal infection. There is paucity of such associations in the literature.


Subject(s)
Bone Marrow Cells/pathology , Hodgkin Disease/pathology , Humans , Leukemia/pathology , Myxomatosis, Infectious/pathology , Necrosis , Retrospective Studies , Stromal Cells/pathology
18.
Colomb. med ; 35(1): 12-17, 2004.
Article in Spanish | LILACS | ID: lil-422819

ABSTRACT

Introducción: Este estudio se realizó con el fin de obtener un acercamiento con la epidemiología geográfica de las neoplasias linfoide en Cali y el suroccidente colombiano, con atención especial en la leucemia linfoma de células T del adulto. Materiales y métodos: Se utilizó información del Registro Poblacional de Cáncer de Cali, de las personas con linfoma no Hodgkin y leucemia linfoides entre 1987 y 1996 procedentes de la costa pacífica. Se obtuvo material incluido en parafina de los pacientes con linfoma no Hodgkin del departamento de Patología, del Hospital Universitario del Valle, con el próposito de investigar secuencias provirales para HTLV-I usando técnicas biomoleculares. Además, se obtuvieron muestras de sangre de voluntarios nativos de Tumaco que representan niveles socioecónomicos bajo, medio y alto, y se analizaron para anticuerpos contra HTLV-I/II con una prueba de imunoabsorción ligada con enzimas (ELISA, Abbott®) y confirmados por Western blot. Resultados: La tasa de incidencia global para linfoma no Hodgkin es al menos dos veces mayor para leucemias en ambos sexos y no ha mostrado cambios notables, mientras la leucemia linfoide ha aumentado en ambos sexos, de manera más notoria en mujeres. La tasa de incidencia para linfoma no Hodgkin aumenta con la edad en ambos sexos. Cinco de 75 casos de linfoma fueron positivos para secuencia de HTLV-I por PCR, en los especímenes tisulares y ninguno de los pacientes tenía prueba serológica para HTLV; 18 (5.1/100) de las 356 personas fueron positivas serológicamente para anticuerpos contra el HTLV-I muestreadas en Tumaco.Conclusiones: Varios casos de ATL pueden pasar desapercibidos en pacientes con linfoma no Hodgkin en quienes la asociación no había sido sospechada. La seroprevalencia de HTLV-I en Tumaco es más alta en mujeres. Merece la pena continuar investigando la intercurrencia con infecciones u otros factores medioambientales que facilitarían una progresión rápida de portadores ATL en habitantes del área


Subject(s)
Health Surveys , Human T-lymphotropic virus 1 , Leukemia/epidemiology , Leukemia/pathology , Lymphoma, T-Cell/epidemiology , Lymphoma, T-Cell/pathology , Colombia
19.
Article in English | WPRIM | ID: wpr-158709

ABSTRACT

Tanshinone II-A is a derivative of phenanthrene-quinone isolated from Salvia miltiorrhiza BUNGE, a traditional herbal medicine that is known to induce antiinflammatory, anti-oxidative and cytotoxic activity. We have examined cellular effects of Tanshione II-A on HL60 human promyelocytic leukemic cells and K562 human erythroleukemic cells. Tanshione II-A induced a dose- and time-dependent DNA fragmentation into the multiples of 180 bp and specific proteolytic cleavage of poly(ADP-ribose) polymerase in both cell lines. PI-staining and flow cytometry analysis of K562 cells following Tanshione II-A treatment showed an increase of the cells possessing hypodiploid DNA indicative of apoptotic state of cells. Caspase-3 activity was significantly increased during Tanshinone II-A treatment of both HL60 and K562 cells, whereas caspase-1 activity was not changed. These results suggest that Tanshione II-A induced HL60 and K562 cellular apoptosis that may be associated with the selective members of caspase family. Copyright 2000 Academic Press.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/chemistry , Apoptosis/physiology , Caspases/metabolism , Caspases/drug effects , Cell Cycle/drug effects , DNA Fragmentation/drug effects , Dose-Response Relationship, Drug , Drugs, Chinese Herbal/pharmacology , Drugs, Chinese Herbal/chemistry , Enzyme Activation/drug effects , HL-60 Cells/pathology , HL-60 Cells/metabolism , HL-60 Cells/drug effects , Humans , Lamiaceae/chemistry , Leukemia/pathology , Leukemia/metabolism , Leukemia/drug therapy , Leukemia, Erythroblastic, Acute/pathology , Leukemia, Erythroblastic, Acute/metabolism , Leukemia, Erythroblastic, Acute/drug therapy , Phenanthrenes/pharmacology , Phenanthrenes/chemistry , Tumor Cells, Cultured
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