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1.
Int. j. med. surg. sci. (Print) ; 8(2): 1-13, jun. 2021. tab, graf
Article in Spanish | LILACS | ID: biblio-1284386

ABSTRACT

Introducción: Las enfermedades cardiovasculares (ECV) son la causa de muerte más frecuente en las mujeres en todo el mundo. La deficiencia de estrógenos debido a la menopausia se asocia con un mayor riesgo a padecer esta patología.Objetivo: Analizar los factores de riesgo cardiovascular en mujeres perimenopausicas con al menos un factor de riesgo cardiovascular modificable mayor (diabetes, hipertensión, dislipemia) con edades comprendidas entre los 45 y 60 años de dos servicios de atención primaria urbanos.Metodología: Estudio descriptivo transversal en dos centros de salud de la ciudad de Ourense (Centro de Salud Novoa Santos y Centro de Salud A Ponte). Las variables analizadas fueron: Actividad estrogénica, tratamiento farmacológico, hábito tabáquico, calidad de vida, índice de masa corporal (IMC), colesterol total, lipoproteína de alta densidad (cHDL), lipoproteína de baja densidad (cLDL) y triglicéridos, tensión arterial sistólica (TAS), tensión arterial diastólica (TAD), frecuencia cardíaca (FC). El estudio contó con la aprobación del Comité Ético de Galicia. El análisis de los datos se efectuó a través del software libre R.Resultados: Participaron 316 mujeres, de las cuales el 64,9% eran menopáusicas. La edad media de la muestra fue de 53,35 ± 4,35 años. El valor medio del IMC fue de 28,125 ± 5,54 Kg/m2. Un 27,8% afirmó presentar hábito tabáquico. El 77,2% de las mujeres de la muestra presentaban niveles de colesterol total ≥200 mg/dl. En relación al cLDL, en el 72,6% de la población estudiada era ≥130 mg/dl. Se observó una correlación estadísticamente significativa entre el IMC y los niveles de triglicéridos, cHDL, TAS, TAD y FC.Conclusiones: Las mujeres perimenopáusicas presentaban una elevada prevalencia hábito tabáquico, obesidad y sobrepeso, a pesar de sufrir al menos un factor de riesgo cardiovascular. Por otra parte mostraron una baja calidad de vida tanto en la dimensión física como mental


Introduction: Cardiovascular disease (CVD) is the most common cause of death in women worldwide. Estrogen deficiency due to menopause is associated with an increased risk of this pathology.Objective: To analyze cardiovascular risk factors in perimenopausal women with at least one major modifiable cardiovascular risk factor (diabetes, hypertension, dyslipemia) between the ages of 45 and 60 of two urban primary care services.Methodology: Cross-sectional descriptive study in two health centers in the city of Ourense (Novoa Santos Health Center and A Ponte Health Center). The variables analyzed were: estrogenic activity, pharmacological treatment, smoking habit, quality of life, body mass index (BMI), total cholesterol, high density lipoprotein (cHDL), low-density lipoprotein (cLDL) and triglycerides, systolic blood pressure (TAS), diastolic blood pressure (TAD), heart rate (FC). The study was approved by the Ethics Committee of Galicia. Data analysis was performed through the free R software.Results: 316 women participated, of whom 64.9% were menopausal. The average age of the sample was 53.35 ± 4.35 years. The average BMI value was 28,125 ± 5.54 Kg/m2. 27.8% said they had a smoking habit. 77.2% of the women in the sample had total cholesterol levels ≥200 mg/dL. In relation to the cLDL, in 72.6% of the population studied it was ≥130 mg/dL. A statistically significant correlation was observed between BMI and triglyceride, cHDL, TAS, TAD and FC levels.Conclusion: Perimenopausal women had a high prevalence of smoking habit, obesity and overweight, despite suffering at least one cardiovascular risk factor. On the other hand they showed a low quality of life in both the physical and mental dimensión.


Subject(s)
Humans , Female , Middle Aged , Primary Health Care , Menopause , Body Mass Index , Heart Disease Risk Factors , Quality of Life , Chi-Square Distribution , Cross-Sectional Studies , Risk Assessment , Lipids/analysis
2.
Prensa méd. argent ; 106(6): 371-378, 20200000. tab, fig
Article in English | LILACS, BINACIS | ID: biblio-1367088

ABSTRACT

Context and Aim: Hematological abnormalities are amongst the most common complications of infection with HIV.There have been quite a few studies on the alterations in lipid profile, too, though the results have largely been inconclusive. The present study was carried-out to assess CD4 cell counts and lipid profile in the HIV infected and AIDS patients in the Indian population and correlates them with the sero-negative controls. Materials and Methods: The present study was designed as a cross-sectional, hospital-based study to assess CD4 cell counts and lipid profile in the HIV infected and AIDS patients in the Indian population and correlates them with the sero-negative controls. Evaluation of lipid profile was done using Erba EM 360, an automated analyzer powered by a diffraction grating photometer while CD4 cell counts were evaluated using Partec Cyflow Counter. Statistical analysis used: The data was analyzed using SPSS version 15.0 (SPSS Inc., Chicago, IL, USA). Comparison of the said parameters was done using Analysis of Variance (ANOVA) and posthoc Games-Howell test. p-value of <0.05 was considered statistically significant. Results: The levels of total cholesterol and low-density lipoproteins (LDLs) were significantly decreased while triglycerides and very low density lipoproteins (VLDLs) were significantly increased in the HIV infected and AIDS patients when compared with the sero-negative controls. Conclusion: Total cholesterol, LDLs, triglycerides and VLDLs were significantly altered in the HIV infected and AIDS patients when compared with the sero-negative controls.


Subject(s)
Humans , CD4 Antigens/immunology , HIV Infections/immunology , Cross-Sectional Studies/statistics & numerical data , Analysis of Variance , HIV Seronegativity/immunology , Dyslipidemias/pathology , Lipids/analysis
3.
Rev. chil. nutr ; 47(3): 351-358, jun. 2020. tab
Article in English | LILACS | ID: biblio-1126131

ABSTRACT

The aim of the present study was to analyze the consumption of foods according to their degree of processing in patients with established atherosclerosis disease. A cross-sectional study was performed with 74 patients of the BALANCE Program trial, a randomized, multicenter and national clinical trial occurring in Brazil. Body weight, height, waist circumference, blood pressure, lipid profile and fasting glucose were collected. Food intake was assessed with 24-h dietary recall. Consumption of nutrients was analyzed in quartiles of consumption of ultra-processed foods and their differences were obtained by one-way ANOVA with Tukey's post hoc test. Half of consumed calories came from natural or minimally processed foods (50.9%), followed by ultra-processed food products (35.1%). The largest contribution in calories came from meats, cereals/roots/tubers, breads, and sweets. No significant difference was found in quartiles of consumption of ultra-processed foods. In this sample, consumption of processed/ultra-processed food was almost the same as natural/minimally processed foods. Preferential consumption of unprocessed/minimally processed foods should be more widely advocated by health professionals.


El objetivo del presente estudio fue analizar el consumo de alimentos, de acuerdo con su grado de procesamiento, en pacientes con enfermedad arterioesclerótica establecida. Estudio transversal, en 74 pacientes del BALANCE Program trial que es un ensayo clínico randomizado, multicéntrico y nacional que se realiza en Brasil. Se midió el peso corporal, la talla, la circunferencia de la cintura, la presión arterial, el perfil lipídico y la glucosa en ayunas. La ingesta de alimentos se evaluó con un recordatorio de 24 horas. El consumo de nutrientes se analizó en cuartiles de consumo de alimentos ultraprocesados y sus diferencias se pusieron a prueba mediante ANOVA de una vía. La mitad de las calorías consumidas provino de alimentos naturales o mínimamente procesados (50.9%), seguidos por productos alimenticios ultraprocesados (35.1%). La mayor contribución en calorías provino de carnes, cereales/raíces/tubérculos, panes y dulces. No se encontraron diferencias significativas en los cuartiles de consumo de alimentos ultraprocesados. En esta muestra, el consumo de alimentos procesados/ultraprocesados fue casi el mismo que de los alimentos naturales/mínimamente procesados. El consumo preferencial de alimentos no procesados/mínimamente procesados debería ser defendido más ampliamente por profesionales de la salud.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Eating , Atherosclerosis , Blood Pressure , Body Height , Body Weight , Brazil , Cardiovascular Diseases , Cross-Sectional Studies , Analysis of Variance , Diet , Waist Circumference , Glucose/analysis , Lipids/analysis
4.
Rev. chil. nutr ; 47(2): 181-189, abr. 2020. tab
Article in English | LILACS | ID: biblio-1115487

ABSTRACT

The objective of this study was to assess the influence of weight gain on the lipid profile of 135 adolescents between 10 - 14 years at baseline and 15 - 19 years at follow-up, enrolled in public schools in Recife, Brazil. The results showed that a BMI z-score correlated with triglycerides (TG) and with high density trigliceride lipopoteine ratio (TG/HDL-c) in males. In females, high z-score correlated with total cholesterol (TC) and low density lipoprotein (LDL-c). In males, for each unit increase in z-score, TG increased by 14.7 mg/dL and the TG/HDL-c ratio increased by 0.4. Among females, TC increased by 9.4 mg/dL, LDL-c increased by 11.6 mg/dL, non-HDL cholesterol increased by 11.8 mg/dL, and HDL-c decreased by 2.3 mg/dL. In males, excessive weight gain was associated with an increase in TG and TG/HDL-c; in females, it was associated with a higher increase in TG/HDL-c and non-HDL cholesterol. However, z-score variation can be a good predictor of lipid profile changes, even in those that are within the normal range.


El objetivo de este estudio fue evaluar la influencia del aumento de peso en el perfil lipídico de 135 adolescentes de edades entre 10 y 14 años de edad al inicio del estudio y de 15 a 19 años en el seguimiento. Los adolescentes pertenecían a escuelas públicas de Recife, Brasil. Los resultados mostraron que el alto puntaje z de indice de masa corporase (IMC) correlacionaba con triglicéridos (TG) y con relación de triglicéridos con lipoproteínas de alta densidad (TG/HDL-c) en los hombres. En las mujeres, puntaje z de IMC se correlacionó con CT y lipoproteína de baja densidad (LDL-c). En los hombres, por cada unidad de aumento en el puntaje z, los TG aumentaron en 14,7 mg/dL y la relación TG / HDL-c aumentó en 0,4; en las mujeres, el CT aumentó en 9,4 mg/dL, el LDL-c aumentó en 11,6 mg/dL, el colesterol no HDL aumentó en 11,8 mg / dL y el HDL-c disminuyó en 2,3 mg/dL. En los hombres, el aumento de peso excesivo se asoció con un aumento de TG y TG/HDL-c; en las mujeres, con un aumento mayor en TG/HDL-c y colesterol no HDL. Sin embargo, la variación z-score puede ser un buen predictor de cambios en el perfil lipídico, incluso en aquellos que se encuentran dentro del rango normal.


Subject(s)
Humans , Male , Female , Child , Adolescent , Weight Gain , Lipids/analysis , Triglycerides/analysis , Brazil , Cardiovascular Diseases/etiology , Body Mass Index , Cholesterol/analysis , Follow-Up Studies , Dyslipidemias/complications , Lipoproteins, HDL/analysis , Lipoproteins, LDL/analysis
5.
Rev. chil. nutr ; 47(2): 281-285, abr. 2020. tab
Article in English | LILACS | ID: biblio-1115499

ABSTRACT

Synthetic preservatives are widely present in processed foods, but most of them have carcinogenic potential, requiring the development of new natural alternatives such as fruit extracts, for microbial control. The objective of the study was to evaluate the chemical characterization, antioxidant, and antimicrobial activity of the sugar apple pulp (Annona squamosa L.). Physicochemical characteristics were evaluated, an extract was prepared, and its antioxidant activity by DPPH method and antimicrobial by disk diffusion. Minimal inhibitory concentration and minimum bactericidal concentration against strains of Salmonella typhimurium, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus were evaluated. The physicochemical analysis revealed that sugar apple pulp had 75.0% moisture, 3.0% ash, 4.0% protein, 0.2% lipids, 3.3% fibers, and 14.5% carbohydrates. The antioxidant activity of the extract by the DPPH method was 20.6%. The pulp extract from the sugar apple had inhibition zone for Staphylococcus aureus, satisfactory inhibitory effect against Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella Typhimurium, but did not present a bactericidal effect. Sugar apple pulp presents adequate levels of nutrients and potential for food application due to its microbiological activity and antioxidant properties.


Los conservantes sintéticos están ampliamente presentes en los alimentos procesados, pero la mayoría tienen potencial carcinogénico, lo que requiere el desarrollo de nuevas alternativas naturales para el control microbiano, como los extractos de frutas. El objetivo del estudio fue evaluar la caracterización química, la actividad antioxidante y antimicrobiana de la pulpa de manzana de azúcar (Annona squamosa L.). Se evaluaron las características fisicoquímicas, y se evaluó su actividad antioxidante mediante el método DPPH y antimicrobiano por difusión en disco, concentración inhibitoria mínima y concentración bactericida mínima contra cepas de Salmonella Typhimurium, Escherichia coli, Listeria monocytogenes y Staphylococcus aureus. El análisis fisicoquímico reveló que la pulpa de manzana de azúcar tiene 75.0% de humedad, 3.0% de cenizas, 4.0% de proteínas, 0.2% de lípidos, 3.3% de fibras y 14.5% de carbohidratos. La actividad antioxidante del extracto por el método DPPH fue del 20.6%. El extracto de pulpa de la manzana de azúcar tenía zona de inhibición para Staphylococcus aureus, efecto inhibidor satisfactorio contra Staphylococcus aureus, Escherichia coli, Listeria monocytogenes y Salmonella Typhimurium, pero no presenta efecto bactericida. La pulpa de manzana de azúcar presenta niveles adecuados de nutrientes y potencial para la aplicación de alimentos debido a su actividad microbiológica y propiedades antioxidantes.


Subject(s)
Plant Extracts/pharmacology , Annona/chemistry , Anti-Bacterial Agents/pharmacology , Antioxidants/pharmacology , Salmonella typhimurium/drug effects , Staphylococcus aureus/drug effects , Carbohydrates/analysis , Plant Extracts/chemistry , Proteins/analysis , Microbial Sensitivity Tests , Escherichia coli/drug effects , Lipids/analysis , Listeria monocytogenes/drug effects , Anti-Bacterial Agents/chemistry , Antioxidants/chemistry
6.
Rev. chil. nutr ; 47(1): 73-79, feb. 2020. tab
Article in English | LILACS | ID: biblio-1092746

ABSTRACT

The study aimed to associate the consumption of trans and saturated fats with dyslipidemia in overweight and obese adolescents who participated in the Study of Cardiovascular Risks in Adolescents (ERICA) from February 2013 to November 2014. Anthropometry, food consumption data, and the lipidogram of 9,538 overweight Brazilian adolescents from macro-regions and Teresina were used in the study. We found an association between saturated fats and HDL-c in Teresina and in the Northeastern macro-region (p-value= 0.002 and p-value= 0.033, respectively), showing that adolescents in Teresina who consumed less than 10% of saturated fats were 2.04-fold more likely to have desirable HDL-c (OR= 2.04), while northeastern adolescents were 0.625 less likely to have desirable HDL-c (OR= 0.625). In Teresina, there was an association between saturated fat consumption and total cholesterol (p-value= 0.045), showing that adolescents consuming less than 10% of saturated fats had a 0.543- fold lower odds of having desirable total cholesterol levels (OR= 0.543). Lipid profile was not associated with consumption of trans fats, but with the consumption of saturated fats. It is important to monitor the lipid profile of adolescents, especially in the presence of overweight.


El objective fue asociar el consumo de grasas trans y saturadas y dislipidemia en adolescentes con sobrepeso y obesidad que participaron en el Estudio de Riesgos Cardiovasculares en Adolescentes (ERICA), en el período de febrero de 2013 a noviembre de 2014. Se utilizaron datos antropométricos, consumo alimentario y lipidograma de 9.538 adolescentes, residentes en Brasil, macrorregiones y Teresina. En Teresina y Macroregión Nordeste, hubo asociación entre el consumo de grasa saturada y HDL-c (p-valor= 0,002 y p-valor= 0,033), respectivamente, mostrando que, adolescentes teresinenses con consumo menor que 10% la grasa saturada tiene 2,04 veces más probabilidades de tener HDL-c deseables (OR= 2,04); mientras que los nordestinos que consumen menos del 10% de grasa saturada tienen 0,625 veces la probabilidad de tener HDL-c deseables (OR= 0,625). En Teresina, hubo asociación entre el consumo de grasa saturada y colesterol total (p-valor= 0,045), mostrando que adolescentes con consumo menor que 10% de grasa saturada tienen 0,543 veces la probabilidad de tener colesterol total deseable (OR= 0,543). El perfil lipídico no se asoció al consumo de grasa trans, sino al consumo de grasa saturada. Es importante realizar monitorización del perfil lipídico de adolescentes, especialmente en presencia del sobrepeso.


Subject(s)
Humans , Male , Female , Child , Adolescent , Dietary Fats , Eating , Dyslipidemias , Overweight , Fatty Acids , Heart Disease Risk Factors , Brazil , Anthropometry , Cholesterol/analysis , Trans Fatty Acids , Lipids/analysis , Obesity
7.
Int. j. morphol ; 38(1): 61-68, Feb. 2020. tab, graf
Article in English | LILACS | ID: biblio-1056398

ABSTRACT

Fruit purees can be added to diet as alternative sources of bioactive compounds for the prevention and/or improvement of the complications of metabolic syndrome. In this work we evaluated the effect of the intake of low-fat diets enriched with fruit purees (guava-strawberry, guava-blackberry, guava-soursop, guava-passion fruit) on the body weight and biochemical markers in metabolic syndrome analogy (MSA)-induced rats. The rats (n=6 for each treatment) were induced with a high fat diet and were injected with streptozotocin, one dose every week for 4 consecutive weeks after fasting overnight, then healthy rats were fed with standard diet and MS rats were fed with standard diet plus each of the fruit puree, for 4 weeks. As novel findings, the diet enriched with fruit purees was associated with a reduction in body weight (~13-21 %) and a control in the metabolism of glucose by decreasing plasma glucose (~5963 %). Also, there was a reduction in the total cholesterol, triacylglycerols, low-density lipoproteins, and low enzymatic activities of alanine aminotransferase, alkaline phosphatase and γ-glutamyl transferase, useful metabolites in the control of inflammatory processes in the liver. A notable improvement in the liver morphology was observed indicating that the treatments had a hepatoprotective effect. The diet enriched with guava-blackberry puree caused the best results on most biochemical markers of MS rats. Therefore, diets enriched with fruit purees can be an alternative for MS individuals for the control and improvement of the complications caused by this syndrome.


Los purés de frutas se pueden agregar a la dieta como fuentes alternativas de compuestos bioactivos para la prevención y / o mejora de las complicaciones del síndrome metabólico. En este trabajo evaluamos el efecto de la ingesta de dietas bajas en grasas, enriquecidas con purés de frutas (guayaba-fresa, guayaba-mora, guayaba-guanábana, guayaba-maracuyá) sobre el peso corporal y los marcadores bioquímicos en el síndrome metabólico (SM) inducido en ratas. Las ratas (n = 6 para cada tratamiento) fueron inducidas con una dieta alta en grasas y se les inyectó estreptozotocina, una dosis cada semana durante 4 semanas consecutivas después de ayunar durante la noche. Luego, las ratas sanas fueron alimentadas con una dieta estándar; y las ratas con SM fueron alimentadas con dieta estándar más cada uno de los purés de frutas, durante 4 semanas. Como hallazgos novedosos, la dieta enriquecida con purés de frutas se asoció con una reducción en el peso corporal (~ 13-21 %) y un control en el metabolismo de la glucosa al disminuir la glucosa en plasma (~ 59-63 %). Además, hubo una reducción en el colesterol total, triacilgliceroles, lipoproteínas de baja densidad, y bajas actividades enzimáticas de alanina aminotransferasa, fosfatasa alcalina y gama-glutamil transferasa, metabolitos útiles en el control de los procesos inflamatorios en el hígado. Se observó una mejora notable en la morfología del hígado, lo que indica que los tratamientos tuvieron un efecto hepatoprotector. La dieta enriquecida con puré de guayaba y mora causó los mejores resultados en la mayoría de los marcadores bioquímicos de las ratas con SM. Por lo tanto, las dietas enriquecidas con purés de frutas pueden ser una alternativa para las personas con SM, para el control y la mejora de las complicaciones causadas por este síndrome.


Subject(s)
Animals , Rats , Diet, Fat-Restricted , Metabolic Syndrome , Fruit , Liver/drug effects , Blood Glucose/drug effects , Body Weight/drug effects , Biomarkers , Albumins/analysis , Disease Models, Animal , Alkaline Phosphatase/analysis , Hepatoprotector Drugs , Transaminases/analysis , Lipids/analysis , Liver/chemistry
8.
Braz. j. med. biol. res ; 53(3): e9201, 2020. tab, graf
Article in English | LILACS | ID: biblio-1089347

ABSTRACT

Methylophiopogonanone A (MO-A), a homoisoflavonoid extracted from Ophiopogon japonicus, has been shown to attenuate myocardial apoptosis and improve cerebral ischemia/reperfusion injury. However, the hypolipidemic effects remain unknown. This study was performed to investigate a potential hypolipidemic effect of MO-A in hyperlipidemia rats, as well as its underlying mechanism of action. A rat model of hyperlipidemia was induced by a high-fat diet (HFD). Animals were randomly divided into three groups (n=8/group): normal control group (NC), HFD group, and HFD+MO-A (10 mg·kg-1·d-1) treatment group. The effects of MO-A on serum lipids, body weight, activity of lipoprotein metabolism enzyme, and gene expression of lipid metabolism were evaluated in HFD-induced rats. In HFD-induced rats, pretreatment with MO-A decreased the body weight gain and reduced serum and hepatic lipid levels. In addition, pretreatment with MO-A improved the activities of lipoprotein lipase and hepatic lipase in serum and liver, down-regulated mRNA expression of acetyl CoA carboxylase and sterol regulatory element-binding protein 1c, and up-regulated mRNA expression of low-density lipoprotein receptor and peroxisome proliferator-activated receptor α in the liver. Our results indicated that MO-A showed strong ability to ameliorate the hyperlipidemia in HFD-induced rats. MO-A might be a potential candidate for prevention of overweight and dyslipidemia induced by HFD.


Subject(s)
Animals , Male , Rats , Ophiopogon/chemistry , Benzodioxoles/pharmacology , Lipid Metabolism , Diet, High-Fat , Hyperlipidemias/prevention & control , Isoflavones/pharmacology , Blotting, Western , Rats, Sprague-Dawley , Disease Models, Animal , Benzodioxoles/isolation & purification , Feces/chemistry , Real-Time Polymerase Chain Reaction , Hyperlipidemias/metabolism , Isoflavones/isolation & purification , Lipids/analysis
9.
Acta bioquím. clín. latinoam ; 53(4): 469-476, dic. 2019. graf, tab
Article in Spanish | LILACS | ID: biblio-1124024

ABSTRACT

El propósito de este estudio fue analizar los cambios post prandiales en el perfil lipídico en respuesta a una comida típica argentina. Se extrajo sangre a 33 mujeres voluntarias después de 12 h de ayuno (T0), 1 h después de un desayuno estandarizado (T1) y 1 h después de un almuerzo estandarizado (T2). Se midieron los niveles de: colesterol total, colesterol de lipoproteínas de alta densidad (C-HDL), colesterol de lipoproteínas de baja densidad (C-LDL) y triglicéridos. Los datos se analizaron utilizando la prueba t de Student pareada. Para cada analito se calculó la diferencia porcentual media (DM%) en T1 y T2 respecto de T0 y se comparó con el valor de referencia del cambio (VRC). Las DM% mayores al VRC se consideraron clínicamente significativas. En T1 y T2, los valores de C-HDL fueron más bajos que en T0, mientras que los valores de C-LDL en T1 fueron más bajos que en T0. Los niveles de triglicéridos fueron significativamente más altos en T1 que en T0. En todos los casos, la variabilidad fue estadísticamente significativa, aunque no clínicamente. En este estudio puede observarse que el perfil de lípidos en T1 y T2 no mostró diferencias clínicamente significativas con respecto a los valores basales.


The purpose of the present study was to analyze postprandial lipid profile changes in response to a typical Argentine meal. Blood was collected from 33 female volunteers after a 12 h fasting period (T0), 1 h after a standardized breakfast (T1) and 1 h after a standardized lunch (T2). The levels of total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides were measured. Data were analyzed using paired Student's t-test. Mean difference % (MD %) was calculated for each analyte at T1 and T2 and was further compared with reference change value (RCV). MDs % higher than RCV were considered clinically significant. At T1 and T2, HDL-C values were lower than at T0, whereas LDL-C values at T1 were lower than at T0. Triglycerides levels were significantly higher at T1 than baseline values. In all cases, variability was statistically, though not clinically, significant. This study demonstrates that at T1 and T2 lipid profile showed no clinically significant differences with respect to basal values.


O objetivo do presente estudo foi analisar as alterações do perfil lipídico pós-prandial em resposta a uma refeição típica argentina. O sangue foi coletado de 33 mulheres voluntárias após um período de jejum de 12 horas (T0),1 h após um café da manhã padronizado (T1) e 1 h após um almoço padronizado (T2). Foram medidos os níveis de: colesterol total (CT), colesterol HDL (C-HDL), colesterol LDL (C-LDL) e triglicérides. Os dados foram analisados utilizando o teste t de Student pareado. A diferença média% (DM%) foi calculada para cada analito em T1 e T2 e foi comparada com o valor de mudança de referência (VRC). Os MDs% maiores que o VRC foram considerados clinicamente significativos. Em T1 e T2, os valores de C-HDL foram menores que em T0, enquanto os valores de C-LDL em T1 foram menores que em T0. Os níveis de triglicérides foram significativamente maiores em T1 do que os valores basais. Em todos os casos, a variabilidade foi estatisticamente, embora não clinicamente, significativa. Este estudo demonstra que no perfil lipídico em T1 e T2 não houve diferenças clinicamente significativas em relação aos valores basais.


Subject(s)
Humans , Triglycerides , Blood , Cholesterol , Fasting , Fasting/blood , Determination , Meals , Breakfast , Pre-Analytical Phase/statistics & numerical data , Lipids , Lipids/analysis , Lipoproteins , Cholesterol, HDL , Cholesterol, LDL , Powders , Referral and Consultation , Coffee , Lunch , Lipoproteins, LDL
10.
Acta bioquím. clín. latinoam ; 53(4): 459-468, dic. 2019. ilus, graf
Article in Spanish | LILACS | ID: biblio-1124023

ABSTRACT

Los profesionales que ejercen la bioquimica clinica son conscientes de la falta de resultados comparables entre laboratorios, independientemente de donde y cuando se realicen. Durante muchos anos el centro de la gestion de la calidad estuvo en la estandarizacion de los procedimientos de medida, la armonizacion va mas alla del metodo y los resultados analiticos e incluye todos los aspectos que hay que tener en cuenta durante el proceso total de la prueba. Los laboratorios de bioquimica clinica han logrado en las ultimas decadas importantes mejoras en la calidad de los procesos analiticos, pero es necesario un esfuerzo mayor dedicado a la vulnerabilidad de los procedimientos extra analiticos para asegurar la comparacion y la concordancia de los resultados obtenidos por diferentes laboratorios clinicos. Las iniciativas destinadas a mejorar la armonizacion de los resultados de laboratorio tienen una dimension etica y de gran importancia en el diagnostico de las dislipemias asociadas al desarrollo de aterosclerosis y la evaluacion del riesgo cardiovascular. Los estudios poblacionales aun muestran dificultades en la identificacion del mejor biomarcador que pueda evidenciar adecuadamente el riesgo cardiovascular en un individuo. La correlacion, discordancia y concordancia muestran que es necesario el diseno de un perfil de pruebas de laboratorio personalizado, con marcadores estandarizados y armonizados, que permita la prediccion del riesgo.


The health professionals who practice clinical biochemistry are aware of the lack of comparable results between laboratories, regardless of where and when they are performed. For many years, the objective of the quality management was the standardization of measurement procedures. The harmonization is beyond the methods and the analytical results, and it includes all the aspects to be taken into account during the whole process of the test. The clinical biochemistry laboratories have achieved important improvements in the quality of the analytical processes in the last decades, but greater effort is necessary for the vulnerability of the extra analytical procedures to ensure the comparison and the agreement of the results obtained by different clinical laboratories. The initiatives aimed to improve the harmonization of laboratory results have an ethical dimension and importance in the diagnosis of dyslipidemia associated with the development of atherosclerosis and the assessment of cardiovascular risk. The population studies still show difficulties in the identification of the best biomarker that can adequately show the cardiovascular risk in an individual. The correlation, discordance and concordance between biomarkers show that it is necessary to design a personalized laboratory test profile, and with standardized and harmonized markers that allow the prediction of risk.


Os profissionais que exercem a bioquímica clínica Clinical estão cientes da falta de resultados comparáveis entre laboratórios, independentemente de onde e quando forem realizados. Por muitos anos, o centro de gestão da qualidade esteve na padronização dos procedimentos de medição, a harmonização vai além do método analítico e dos resultados analíticos e inclui todos os aspectos a considerar durante o processo do teste. Laboratórios bioquímica clínica têm alcançado, nas últimas décadas grandes melhorias na qualidade dos processos analíticos, mas precisa de um esforço maior dedicado à vulnerabilidade dos procedimentos extra-analíticos, para garantir a comparação e concordancia dos resultados obtidos pelos diferentes laboratórios clínicos. Iniciativas para melhorar a harmonização dos resultados laboratoriais têm uma dimensão ética e de grande importȃncia no diagnóstico de dislipidemias associadas ao desenvolvimento de aterosclerose e à avaliação do risco cardiovascular. As pesquisas populacionais mostram ainda dificuldades em identificar o melhor biomarcador que possa demonstrar em forma adecuada o risco cardiovascular em um individuo, a correlação, discordância e concordância mostram que é necessário o desenho de um perfil de testes personalizado, com marcadores padronizados e harmonizada, que permite a previsão de risco.


Subject(s)
Humans , Reference Standards , Biomarkers , Quality Management , Diagnosis , Laboratories , Lipids , Lipids/analysis , Methods , Biochemistry , Health , Risk , Atherosclerosis , Dyslipidemias , Ethics , Laboratory Test , Forecasting
11.
Arch. latinoam. nutr ; 69(3): 157-164, sept. 2019. tab
Article in Spanish | LILACS, LIVECS | ID: biblio-1053250

ABSTRACT

El ayuno intermitente es una estrategia nutricional de creciente interés para el control del peso y mejora de la salud metabólica. El objetivo del presente estudio fue evaluar el efecto de un protocolo de ayuno intermitente sobre la composición corporal, perfil lipídico y los biomarcadores de riesgo cardiovascular en estudiantes universitarios. En este estudio experimental participaron 30 sujetos varones y fueron divididos aleatoriamente en dos grupos, 15 sujetos constituían el grupo experimental (GE) (edad: 20,83±0,98 años) y 15 el grupo control (GC) (edad: 23,71±5,55 años). El GE realizó un protocolo de ayuno 16/8, dieciséis horas de ayuno y ocho horas de ingesta calórica sin limitaciones, dos días consecutivos a la semana durante cinco semanas. Se evaluó la composición corporal, la ingesta calórica, el perfil lipídico y los biomarcadores del riesgo cardiovascular al inicio, mitad y final del protocolo. Se observaron descensos significativos en el GE en pliegues cutáneos, perímetro cintura, porcentaje de grasa, perfil lipídico y biomarcadores del riesgo cardiovascular en comparación con GC (p<0,05). Se encontraron incrementos significativos en la ingesta de colesterol y ácidos grasos poliinsaturados en el GE al final del estudio (p<0,05). Se observaron descensos en el colesterol total, triglicéridos, lipoproteínas de baja densidad y biomarcadores del riesgo cardiovascular a lo largo del protocolo en el GE (p<0,05). Un protocolo de ayuno intermitente 16/8, dos días consecutivos por semana, durante cinco semanas, parece efectivo para mejorar parámetros de composición corporal y perfil lipídico, así como para mejorar los biomarcadores relacionados con el riesgo cardiovascular(AU)


Intermittent fasting is a nutritional strategy of high interest in weight control and improvement of metabolic health. The objective of this study was to evaluate the effect of an intermittent fasting protocol on body composition, lipid profile and biomarkers of cardiovascular risk in university students. In this experimental study thirty male subjects participated and were randomly divided into two groups; fifteen subjects constituted the experimental group (GE) (age: 20.83 ± 0.98 years) and fifteen the control group (GC) (age: 23.71 ± 5.55 years). The GE performed a fasting protocol 16/8, sixteen hours of fasting and eight hours of caloric intake without limitations, two consecutive days per week for five weeks. Body composition, calorie intake, lipid profile and biomarkers of cardiovascular risk were evaluated at the beginning, middle and at the end of the protocol. Significant decreases were found in GE in skinfolds, waist perimeter, % fat, lipid profile and biomarkers of cardiovascular risk as compared to GC (p <0.05). There were significant increases in the intake of cholesterol and polyunsaturated fatty acids in the GE at the end of the study (p <0.05). There were decreases in total cholesterol, triglycerides, low-density lipoproteins, and biomarkers of cardiovascular risk throughout the study in GE (p<0.05). An intermittent fasting protocol 16/8, two consecutive days per week, for five weeks, seems effective to improve parameters of body composition and lipid profile, as well as to improving biomarkers related to cardiovascular risk(AU)


Subject(s)
Humans , Male , Adult , Fasting/adverse effects , Feeding Behavior , Lipids/analysis , Body Composition , Nutritional Status , Food Composition
12.
Arch. latinoam. nutr ; 69(1): 34-41, mar. 2019. ilus, tab
Article in Spanish | LILACS, LIVECS | ID: biblio-1022501

ABSTRACT

La alteración de los lípidos en sangre o dislipidemias sobre todo el colesterol y triglicéridos, son un factor de riesgo de ateroesclerosis y enfermedades cardiovasculares. En este trabajo se analizó el efecto del consumo de huevo, clara, yema de huevo, mantequilla y maní sobre el perfil lipídico de ratones sanos. Se utilizaron seis grupos de ratones hembras sanas, el grupo control (GC) recibió balanceado comercial, mientras que en los demás grupos, 15% de su alimentación diaria consistió en huevo entero (GHE), yema (GY), clara de huevo (GCH), manteca o mantequilla (GMT) o maní (GMN), durante 28 días. Al final de este periodo, se obtuvo la muestra de suero para la determinación de los niveles de colesterol total (CT), triglicéridos (TG), colesterol HDL, colesterol LDL y colesterol VLDL, y se calcularon los índices de riesgo aterogénico y de riesgo cardiaco. Se observó un aumento estadísticamente significativo en los niveles de CT y LDL en relación al GC en los grupos GHE (p<0,0001), GY (p<0,0001), GCH (p<0,005) y GMT (p<0,001), mientras que en niveles de TG presentan un aumento los grupos GHE (p<0,0001) y GY (p<0,0001). El nivel de HDL aumentó significativamente en los grupos GHE (p<0,005) y GMT (p<0,001), y en cuanto al índice de riesgo cardiaco y aterogénico el grupo GY fue el único que reveló aumento significativo (p<0,0001). En el grupo que recibió maní (GMN), no se alteraron los niveles de lípidos, y por lo tanto tampoco el riesgo cardiaco ni aterogénico, lo que se traduce en un menor riesgo de enfermedades cardiovasculares de este grupo(AU)


The alteration of blood lipids or dyslipidemias, especially cholesterol and triglycerides, are a risk factor for atherosclerosis and cardiovascular diseases. In this work, the effect of consumption of egg, egg white, egg yolk, butter and peanut on the lipid profile of healthy mice was analyzed. Six groups of healthy female mice were used, the control group (GC) received commercial pellets, while for the other groups, 15% of their daily food consisted of whole egg (GHE), egg yolk (GY), egg white (GCH) ), butter (GMT) or peanut (GMN), for 28 days. At the end of this period, a serum sample was obtained for the determination of the levels of total cholesterol (CT), triglycerides (TG), HDL cholesterol, LDL cholesterol and VLDL cholesterol, and cardiac and atherogenic risk index were calculated. A statistically significant increase in TC and LDL levels was observed in relation to GC in the GHE (p <0,0001), GY (p <0,0001), GCH (p <0,005) and GMT (p <0,001) groups; whereas TG levels showed an increase in the GHE (p <0,0001) and GY (p <0,0001) groups. The HDL level increased significantly in the groups GHE (p <0,005) and GMT (p <0,001), and in terms of the cardiac and atherogenic risk index, the GY group was the only group that revealed a significant increase (p <0,0001). In the group that received peanuts (GMN), the lipid levels were not altered, and therefore neither the cardiac nor the atherogenic risk, which indicates the risk of cardiovascular diseases in this group, were affected(AU)


Subject(s)
Rats , Atherosclerosis/physiopathology , Dyslipidemias/complications , Lipids/analysis , Cholesterol, HDL , Food Analysis , Nutritive Value
13.
São Paulo; s.n; s.n; 2019. 177 p. graf, tab.
Thesis in English | LILACS | ID: biblio-1026605

ABSTRACT

Lipids are a diverse and ubiquitous group of compounds, which have several biological functions such as structural components of cell membranes, energy storage, and participation in signaling pathways. Free radicals or reactive oxygen species could attack polyunsaturated fatty acid esterified to phospholipids generating oxidized products. Once oxidized, lipids are able to modify amino acids residues in proteins leading to modulation signaling pathways and cellular redox balance. Furthermore, alteration of lipid homeostasis is also linked to development and progression of neurodegenerative diseases. The purposes of this study were (i) to investigate the role of lipids in protein aggregation, (ii) to investigate the plasma lipidome of an ALS rat model (SOD1G93A rats), and (iii) to investigate the effect of high-fat diet in plasma lipidome of an ALS rat model. In chapters 1 and 2, the interaction between cytochrome c (cytc) and cardiolipin hydroperoxide (CLOOH), as well as cholesterol hydroperoxide (ChOOH) promoted protein aggregation. Mass spectrometry analysis of tryptic peptides from CLOOH-containing reaction revealed K72 and H26 consistently modified by 4- hydroxynonenal (4-HNE). Further, adduction of K27, K73 and K88 were detected with 4- oxynonenal (4-ONE). For the first time, we characterized the dityrosine cross-linked peptides at Y48-Y74, Y48-97 and Y74-Y97 in oligomeric cytc. Similarly, ChOOH-containing reaction showed dityrosine cross-linked peptides at Y48-Y48, Y48-Y74 and Y48-Y97 in dimeric cytc. In accordance to previous studies, the proposed mechanism under covalent protein oligomerization mediated by lipid hydroperoxide could be related to modification of lysine and tyrosine residues. In chapter 3, we characterized the lipid composition of blood plasma in amyotrophic lateral sclerosis (ALS), since dysregulation of lipid metabolism is increasingly associated with neuropathology. Using untargeted lipidomics approach based on liquid chromatography coupled to mass spectrometry, we found main alterations in triglycerides, phospholipids and sphingolipids in symptomatic ALS rats relative to controls. Additionally, for the first time we reported acylceramides species in the plasma. In order to investigate the source of these lipid alterations, we analyzed the lipid content of fractioned lipoproteins. Triglycerides and phospholipids were found in very low-density lipoprotein (VLDL), while acylceramides and hexosylceramides were found enriched in high-density lipoprotein (HDL). In chapter 4, high-fat diet containing lard or high-fish oil as much as 60% of total lipids has both the largest change on plasma lipid composition. Overall survival was not statistically different when compared to control diet. Increased levels of acylceramides, hexosylceramides and acylcarnitines were observed in ALS rats fed a control diet or high-fat diet in comparison to WT controls. Importantly, untargeted lipidomic analysis of blood plasma highlighted acylceramide d18:1/24:1+20:4 as potential biomarkers of ALS progression. Thus, our lipidomic analysis provides a novel insight into the molecular level event driving molecular dysregulation in ALS. Additional research is needed to determine the effect of plasma lipid alteration on motor neuron process and energetic metabolism. Collectively, our findings reinforce the idea that lipids play a relevant role in modulating cellular processes linked to protein aggregation and neurodegeneration


Os lipídeos são moléculas que possuem várias funções biológicas importantes, atuando como componente de membranas celulares, servindo com fonte de reserva de energia e participando de vias de sinalização. Os ácidos graxos poli-insaturados esterificados aos fosfolipídeos, por exemplo, são potenciais alvos para o ataque de radicais livres gerando produtos oxidados que são capazes de modificar resíduos de aminoácidos em proteínas levando a modulação das vias de sinalização e balanço redox. Por outro lado, alteração na homeostase do metabolismo dos lipídeos está relacionada ao desenvolvimento e progressão de doenças neurodegenerativas. Tendo em vista a importância dos lipídeos nos processos biológicos, os objetivos desse estudo foram (i) investigar o papel dos lipídeos na agregação proteica (capítulo 1 e 2), (ii) investigar as alterações na composição lipídica do plasma de rato modelo SOD1G93A de esclerose lateral amiotrófica (ELA) (capítulo 3) e (iii) investigar o efeito da suplementação de dietas hiperlipídicas na composição lipídica do plasma de rato modelo SOD1G93A (capítulo 4). No capítulo 1 e 2, a interação do citocromo c (citc) com hidroperóxido de cardiolipina (CLOOH) e hidroperóxido de colesterol (ChOOH) promove a agregação covalente do citc. Análise por nLC-MS/MS dos peptídeos digeridos identificou resíduos de lisina (K72) e histidina (H26) modificado por 4-hidroxininenal (4-HNE), enquanto os resíduos K27, K73 e K88 foram modificados por 4-oxinonenal (4-ONE). Pela primeira vez, nós caracterizamos ditirosinas (Y48-Y74, Y48-97 e Y74-Y97) na reação do citc com CLOOH. Também foram caracterizadas ditirosinas envolvendo os resíduos Y48-Y48, Y48-Y74 e Y48-Y97 na reação com ChOOH. Esses resultados corroboram com estudos anteriores que sugerem um mecanismo de agregação proteica envolvendo a perda da carga positiva de lisina e formação de ditirosina pela combinação de radicais de tirosil. No capítulo 3, a análise da composição lipídica do plasma de ratos SOD1G93A utilizando LC-MS/MS revelou alterações significativas na composição de triglicérides, glicerofosfolipídeos e esfingolipídeos em ratos sintomáticos comparado com os assintomáticos. É importante destacar que pela primeira vez acilceramidas foram identificadas em plasma de rato modelo para ALS. Análise da composição lipídica de lipoproteínas isoladas, maior fonte de lipídeos circulantes no plasma, mostraram alterações de triglicérides e glicerofosfolipídeos em VLDL. As acilceramidas e as hexosilceramidas, por sua vez, foram encontradas em maior abundância em HDL. No capítulo 4, a suplementação com dietas hiperlipídicas (rica em banha de porco e óleo de peixe) alterou significativamente o perfil lipídico do plasma em relação a doença. Contudo, não foi observado aumento significativo na sobrevida dos ratos ALS comparado com dieta controle. Independente da dieta, a concentração plasmática de acilcarnitina, hexosilceramidas e acilceramidas foram significativamente aumentadas em ratos ALS comparado com WT. A análise do perfil lipídico do plasma mostrou que a acilceramida d18:1/24:1+20:4 pode ser um potencial marcador de progressão da ALS. Dessa forma, os resultados mostrados fornecem uma visão enriquecedora sobre o evento a nível molecular que conduz a desregulação lipídica na ELA. Coletivamente, nossos resultados reforçam a importância dos lipídeos na modulação dos processos celulares ligados a agregação de proteínas e na neurodegeneração


Subject(s)
Animals , Male , Rats , Amyotrophic Lateral Sclerosis/physiopathology , Lipids/analysis , Mass Spectrometry/instrumentation , Diet, High-Fat/adverse effects , Protein Aggregation, Pathological/classification
14.
Rev. medica electron ; 40(6): 1835-1855, nov.-dic. 2018. tab
Article in Spanish | LILACS, CUMED | ID: biblio-978706

ABSTRACT

RESUMEN Introducción: las enfermedades cardiovasculares actualmente son la principal causa de muerte en los países industrializados y se espera que también lo sean en los países en vías de desarrollo en el año 2020. Entre ellas la enfermedad arterial coronaria es la manifestación más prevalente, y se asocia a alta morbimortalidad. La ateroesclerosis es su principal génesis y por tanto los trastornos del metabolismo de los lípidos. Objetivo: determinar el perfil lipídico en pacientes egresados por síndrome coronario agudo. Materiales y métodos: se realizó una investigación descriptiva, transversal, correlacional en los pacientes egresados del servicio de cardiología del hospital Faustino Pérez en el período comprendido entre enero y diciembre del 2015. Resultados: se observó un aumento de los lípidos colesterol, triglicéridos, LDL-c, VLDL y Lp (a) en más del 50% de los pacientes estudiados. Los índices de Apo B/Apo A y Col/HDL-c marcaron como indicador de alto riesgo en un 67,5 y 58,75 % respectivamente. La HDL-c considerada como factor protector, resultó disminuida en más del 50% de los pacientes. Conclusiones: se detectó correlación entre el síndrome coronario agudo y trastornos en el metabolismo de los lípidos. Se recomienda realizar estudios observacionales epidemiológicos para determinar la verdadera influencia de estos factores como agente causal de síndrome coronario agudo (AU).


ABSTRACT Introduction: Currently, cardiovascular diseases are the main cause of death in developed countries, and it is believed that they also will be the same in developing countries in 2020 year. Among them, coronary arterial disease is the most prevalent manifestation, and is associated to high morbi-mortality. Atherosclerosis is its most important cause and consequently the disorders of the lipids metabolism. Objective: determining the lipid profile in patients discharged for acute coronary syndrome. Materials and methods: a descriptive, cross-sectional, correlational research was carried out in patients discharged from the service of Cardiology of the hospital Faustino Perez in the period from January to December 2015. Results: it was found an increase of the lipids cholesterol, triglycerides, LDL-c, VLDL and Lp(a) in more than 50 % of the studied patients. The indexes of Apo B/Apo A and Col/HDL-c were high risk indicators in 67.5 and 58.75 % respectively. HDL-c, considered as a protecting factor, was diminished in more than 50 % of the patients. Conclusions: a correlation was found between the acute coronary syndrome and disturbances in the lipids metabolism. We recommend performing epidemiologic, observational studies to determine the real influence of these factors as causal agent of the acute coronary syndrome (AU).


Subject(s)
Humans , Cardiovascular Diseases , Risk Factors , Morbidity , Acute Coronary Syndrome/epidemiology , Lipids/analysis , Coronary Artery Disease , Cardiology , Epidemiology, Descriptive , Cross-Sectional Studies , Disease Prevention , Free Radicals/blood , Correlation of Data , Life Style
15.
Int. j. morphol ; 36(3): 1016-1021, Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-954224

ABSTRACT

To determine the effects of 70 % ethanolic extract of Eleutherine bulbosa (Mill.) L. bulbs contained naphtoquinone in blood pressure and lipids profile level of ovariectomized rats. Thin layer chromatography was performed to analyze the content of the Eleutherine bulbosa (Mill.) bulbs extracts using Chloroform : Methanol (7:1) as eluent. This study used white female Sprague-Dawley rats which were divided into 6 groups: SHAM and OVX, both given 0.5 % carboxymethyl cellulose; the positive control group that was given tamoxifen (0.36 mg/200 g B.W.); the three various of doses of extract namely dose 1, 2, and 3 that were given 8, 12, and 18 mg/200 g BW respectively on day 29 until 50. All groups were ovariectomized on day 1, except sham group. Measurement of blood pressure were performed on the day before ovariectomy using non-invasive tool CODA®; and on the second, third, and fourth weeks post-ovariectomy. Three weeks after treatment of hypoestrogen rats by the dose 18 mg/200 g B.W, Eleutherine bulbosa extracts could decrease the systolic blood pressure level to 28.06 %, the diastolic to 30.47 %. Lipid profile of Dose 3 also showed recovery of Triglyceride, LDL and also Total Cholesterol. Eleutherine bulbosa extracts could improve blood pressure of ovariectomized rats by controlling lipids profile level.


Para determinar los efectos del 70 % de extracto etanólico de Eleutherine bulbosa (Mill.) L. en bulbos que contenían naftoquinona, se analizó la presión arterial de ratas ovariectomizadas. Se realizó un estudio de cromatografía de capa fina para analizar el contenido de los extractos de bulbos de Eleutherine bulbosa (Mill.) usando cloroformo:metanol (7:1) como diluyente. En este estudio se utilizaron ratas blancas Sprague-Dawley hembras, que se dividieron en 6 grupos: SHAM y OVX, ambos grupos fueron administrados con carboximetilcelulosa al 0,5 %; grupo control positivo, recibió tamoxifeno (0,36 mg / 200 g de B.W.); y tres grupos restantes, que recibieron tres dosis diferentes de extracto, es decir, dosis 1, 2 y 3 a las que se les administró 8, 12 y 18 mg / 200 g de por peso, respectivamente el día 29 hasta el día 50. Todos los grupos fueron sometidos a ovariectomía en el día 1, excepto el grupo simulado. La medición de la presión arterial se realizó el día anterior a la ovariectomía con la herramienta no invasiva CODA®, y, posteriormente, en la segunda, tercera y cuarta semanas de realizada la ovariectomía. Tres semanas después del tratamiento aplicado a las ratas hipoestrógenas, con la dosis de 18 mg / 200 g por peso, los extractos de Eleutherine bulbosa podrían disminuir el nivel de presión arterial sistólica al 28,06 %, y la diastólica al 30,47 %. La dosis 3 del perfil lipídico mostró mejoría en los niveles de Triglicéridos, LDL y Colesterol Total. Los extractos de Eleutherine bulbosa podrían mejorar la presión sanguínea de ratas ovariectomizadas controlando el nivel del perfil lipídico.


Subject(s)
Animals , Female , Rats , Blood Pressure/drug effects , Plant Extracts/pharmacology , Naphthoquinones/pharmacology , Iridaceae/chemistry , Plant Extracts/chemistry , Ovariectomy , Chromatography, Thin Layer , Naphthoquinones/analysis , Rats, Sprague-Dawley , Estrogens/deficiency , Lipids/analysis
16.
Rev. Paul. Pediatr. (Ed. Port., Online) ; 36(3): 286-291, jul.-set. 2018. tab
Article in Portuguese | LILACS | ID: biblio-977060

ABSTRACT

RESUMO Objetivo: Avaliar se o conteúdo de gordura e o valor energético estimado no colostro diferem em função da idade gestacional e do crescimento fetal. Métodos: Estudo transversal com mães de recém-nascidos pré-termo e a termo nascidos em centro terciário em 2015-2016. Critério de inclusão: gestação única, sem diabetes, corioamnionite e mastite, sem uso de drogas ilícitas e álcool, ausência de malformação ou infecção congênita fetal. Foram constituídos quatro grupos conforme idade gestacional e crescimento fetal: pré-termo pequeno para a idade gestacional (n=33); pré-termo adequado (n=60); a termo pequeno (n=59) e a termo adequado para a idade gestacional (controle, n=73). O colostro foi coletado por extração manual entre 24 e 72 h pós-parto. Foram analisadas variáveis gestacionais e de nascimento. Os desfechos foram o conteúdo de gordura no colostro, pelo método do crematócrito, e o valor energético estimado. Na comparação entre grupos foram utilizados os testes do qui-quadrado ou Exato de Fisher, ANOVA e regressão linear multivariada. Resultados: A idade gestacional média foi de 34 semanas nos neonatos pré-termo e 39 semanas nos a termo. O crematócrito não diferiu entre os grupos, com valores médios de 3,3 a 4,0%; e o valor energético estimado foi de 52 a 56 kcal/dL. Crematócrito ≥4% foi mais frequente no grupo a termo pequeno para idade gestacional. Apenas no grupo de recém-nascidos pré-termo pequenos para a idade gestacional houve correlação entre crematócrito e índice de massa corpórea materno. Conclusões: O conteúdo de gordura e o valor energético estimado do colostro não diferiram em função da idade gestacional e do crescimento fetal.


ABSTRACT Objective: To determine whether fat content and energy value change in colostrum according to gestational age and fetal growth. Methods: Cross-sectional study with mothers of preterm and term infants born in a tertiary center in 2015-2016. Inclusion criteria: single pregnancy, absence of diabetes, chorioamnionitis and mastitis, no use of illicit drugs or alcohol, without fetal congenital malformation or infection. Four groups were formed according to gestational age and fetal growth: preterm infants small for gestational age (PT-SGA; n=33) and appropriate for gestational age (PT-AGA; n=60), term infants small for gestational age (T-SGA; n=59) and appropriate for gestational age (T-AGA; control, n=73). Colostrum was collected between 24-72 hours postpartum. Gestational and birth variables were analyzed. Outcome variables were: fat content in colostrum (evaluated by crematocrit method) and estimated energy value. Chi-square or Fisher exact tests, ANOVA, and multivariable linear regression were used for comparison among groups. Results: Mean gestational age was 34 weeks in preterm infants and 39 weeks in term neonates. Crematocrit did not differ between groups, with mean values varying between 3.3 and 4.0%; estimated energy value was 52 to 56 kcal/dL. Crematocrit ≥4% was more frequent in the T-SGA group. Only in the PT-SGA group there was a correlation between crematocrit and body mass index of the mother. Conclusions: The fat content and energy value of colostrum did not change according to gestational age or fetal growth.


Subject(s)
Humans , Female , Infant, Newborn , Adult , Young Adult , Gestational Age , Colostrum/chemistry , Fetal Development/physiology , Lipids/analysis , Cross-Sectional Studies
17.
An. acad. bras. ciênc ; 90(1): 485-494, Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-886916

ABSTRACT

ABSTRACT Soybean (Glycine max L.) seed contains amounts of protein, lipid, carbohydrate and mineral elements, which protein and lipid have been known as a main part for soybean's trade value. In this study, in order to investigate the effect of ferrous nano-oxide particles on nutritional compounds of soybean seed, an experiment with 5 treatments and 3 replications was conducted as a randomized complete block design. Treatments were 5 concentrations of ferrous nano-oxide particles including 0, 0.25, 0.5, 0.75 and 1 g L-1 which were sprayed 3 times at 4 and 8 leaves stage and pod initiation. Lipid and protein contents, fatty acids profile, some of mineral elements such as Fe, Mg, Ca and P, chlorophyll a, b and total chlorophyll content were determined. Results showed that solution containing ferrous nano-oxide particles had significant effect on nutritional compounds of soybean seed (P<0.01) compared to control. The highest content of lipid and protein (25.4 and %33.8, respectively) observed by applying 0.75 g L-1 of ferrous nano-oxide and the lowest content was also in control. Changes in the trends of fatty acids profile (palmitic, oleic, linoleic and linolenic acids), some of mineral elements (Fe, Mg, Ca and P) and chlorophyll contents were similar to lipid and protein levels which by increasing in concentration of ferrous nano-oxide from 0 to 0.75 g L-1 all measured parameters also increased, but reduction in all parameters was observed in concentration from 0.75 to 1 g L-1. In conclusion, application of 0.75 to 1 g L-1 ferrous nano-oxide had the best effect on the nutrient composition of soybean seed.


Subject(s)
Seeds/drug effects , Seeds/chemistry , Soybeans/drug effects , Soybeans/chemistry , Ferrous Compounds/pharmacology , Metal Nanoparticles/chemistry , Reference Values , Seeds/physiology , Soybeans/physiology , Trace Elements/analysis , Proteins/analysis , Random Allocation , Chlorophyll/analysis , Regression Analysis , Reproducibility of Results , Fatty Acids/analysis , Fertilizers , Lipids/analysis
18.
An. acad. bras. ciênc ; 90(1): 325-332, Mar. 2018. tab, graf
Article in English | LILACS | ID: biblio-886914

ABSTRACT

ABSTRACT We aimed to evaluate the frequency of bee pollen production, its botanical origin and chemical composition when collected in different seasons. Our results indicate that higher proteins (22.80 ± 3.09%) and flavonoids (2789.87 ± 1396.00 μg 100g-1) levels were obtained in the winter season, which also showed greater pollen production (134.50 ± 35.70 grams) and predominance of the Myrtaceae family. As for spring we found high concentrations of lipids (4.62 ± 2.26%) and low ash content (2.22 ± 0.39%). Regarding the amino acid composition and vitamin C content, we found no differences between the averages throughout the seasons. Our results highlight the importance of understanding not only the botanical origin and the chemical composition of bee pollen, but also the harvesting frequency of this product by bees, so that it becomes possible to supplement the colonies in times of natural food resources shortage.


Subject(s)
Animals , Pollen/physiology , Pollen/chemistry , Seasons , Bees/physiology , Magnoliopsida/physiology , Pollen/classification , Ascorbic Acid/analysis , Reference Values , Flavonoids/analysis , Proteins/analysis , Analysis of Variance , Amino Acids/analysis , Lipids/analysis , Animal Nutritional Physiological Phenomena
19.
Rev. bras. cir. plást ; 33(1): 48-55, jan.-mar. 2018. ilus, tab
Article in English, Portuguese | LILACS | ID: biblio-883637

ABSTRACT

Introdução: Desde a introdução da técnica de lipoaspiração, a melhora do contorno corporal vem ganhando novos horizontes. O uso da lipoaspiração superficial passou a ser usado a favor da estética e, com isso, o uso de tecnologias, tais como o VASER e o laser de Diodo, nos comprimentos de 915 e 980 nn, diminuem as complicações, com melhora da dor pós-operatória, tempo de cicatrização e retração cutânea. Métodos: Foram estudados, inicialmente, 16 pacientes submetidos à laserlipólise de alta definição, entre o período de 2014 e 2016. O nível de satisfação em relação ao resultado foi questionado aos pacientes. Resultados: A maioria dos pacientes foi do sexo masculino, idade média 39 anos, com média de dor de 2,3 de na escala de visual de dor (0 a 10), apresentando resultados duradouros após 1 ano, sem complicações e retorno às suas atividades de trabalho e físicas precocemente. Conclusões: Desta forma, o uso do laser de Diodo com comprimentos de ondas de 915 e 980 nm, associado à Lipoescultura de Alta Definição, promoveu resultados satisfatórios, promissores devendo ser testado numa amostragem maior de pacientes.


Introduction: Since the introduction of the liposuction technique, the improvement of body contour has been gaining new horizons. Superficial liposuction has been used in favor of aesthetics; the use of technologies, such as VASER and diode laser at 915- and 980-nm wavelengths, helps reduce complications and improves post-time, healing time, and cutaneous retraction. Methods: Fourteen patients submitted to high-resolution laser lipolysis were studied between 2014 and 2016. The level of patient satisfaction regarding the treatment outcome was assessed. Results: The majority of the patients were men, with a mean age of 39 years and a mean pain score of 2.3 on the visual pain scale (0 to 10), presenting lasting results after 1 year, with no complications and an early return to work and physical activities. Conclusions: The use of diode laser at the wavelengths of 915 and 980 nm in high-definition liposculpture promoted satisfactory, promising results and should be tested in a larger sample of patients.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Young Adult , Surgical Procedures, Operative , Lipectomy , Patient Satisfaction , Esthetics , Lasers , Lipolysis , Surgical Procedures, Operative/methods , Lipectomy/adverse effects , Lipectomy/instrumentation , Lipectomy/methods , Esthetics/psychology , Lipid Metabolism , Lasers/adverse effects , Lipids , Lipids/analysis
20.
Neumol. pediátr. (En línea) ; 13(1): 11-14, ene. 2018. ilus
Article in Spanish | LILACS | ID: biblio-999205

ABSTRACT

Aspiration pulmonary disease is the condition in which there is a passage of gastric or oral content to the airways; the extent of aspiration is conditioned by the amount and frequency with which it occurs. The diagnostic approach of this pathology begins with an adequate clinical history with special emphasis on pathologies that generate poor swallowing mechanics such as neurological diseases, esophageal atresia and patients with a history of gastroesophageal reflux. The studies used for the diagnosis of this syndrome are chest X-ray, swallowing mechanics evaluation with barium, Ph-metry, scintigraphy with Tc99 for gastroesophageal reflux, delayed pulmonary scanning, endoscopic study of the digestive tract and bronchoscopy in search of lesions in the respiratory mucosa and bronchial lavage to detect lipid-laden alveolar macrophages which have been linked to the presence of aspiration syndromes in several publications


La neumopatía por aspiración es la condición en la que existe un paso de contenido gástrico a la vía aérea. La magnitud de la aspiración depende de la cantidad y la frecuencia con la que se presenta. El abordaje diagnóstico de esta patología se inicia con una historia clínica adecuada con especial énfasis en patologías que generen una mala mecánica de deglución como pueden ser enfermedades neurológicas, atresia esofágica y pacientes con antecedentes de reflujo gastroesofágico. Los estudios que se requieren para el diagnóstico de esta enfermedad constan de radiografía de tórax, estudio de deglución, Ph-metria, gamagrama para reflujo, estudio endoscópico de vía digestiva y broncoscopia en búsqueda de lesiones en la mucosa respiratoria y realización de lavado bronquial para macrófagos alveolares cargados de lípidos


Subject(s)
Humans , Child , Pneumonia, Aspiration/diagnosis , Pneumonia, Aspiration/etiology , Pneumonia, Aspiration/diagnostic imaging , Gastroesophageal Reflux/complications , Lipids/analysis
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