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An. bras. dermatol ; 95(1): 40-45, Jan.-Feb. 2020. tab
Article in English | LILACS | ID: biblio-1088733


Abstract Background: Psoriasis is a chronic immune-mediated inflammatory skin disease that is associated with cardiovascular comorbidities. Objectives: The objective of this retrospective study is to assess the C-reactive protein, monocyte-to-high-density-lipoprotein ratio, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio as inflammatory markers in patients with psoriasis and to search for a relationship between these parameters and psoriasis severity, as defined by the psoriasis area and severity index. Methods: There were 94 patients with psoriasis and 118 healthy controls enrolled in the study. The C-reactive protein, monocyte-to-high-density-lipoprotein ratio, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio values of two groups were retrospectively evaluated. Results: Statistically significant differences were observed in terms of C-reactive protein, monocyte-to-high-density-lipoprotein ratio, neutrophil-to-lymphocyte ratio and monocyte-to-lymphocyte ratio between the patient and control groups (p = 0.001, p = 0.003, p = 0.038, and p = 0.007, respectively). Positive correlations were found between the psoriasis area and severity index and the values of C-reactive protein, monocyte-to-high-density-lipoprotein ratio, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio (r: 0.381; p < 0.01, r: 0.203; p < 0.05, r: 0.268; p < 0.01, r: 0.374; p < 0.01, r: 0.294; p < 0.01, respectively). Study limitations: The small sample size and the retrospective design of the study are limitations. Conclusion: Elevated C-reactive protein, monocyte-to-high-density-lipoprotein ratio, neutrophil-to-lymphocyte ratio, and monocyte-to-lymphocyte ratio were significantly associated with psoriasis. A positive correlation between C-reactive protein and monocyte-to-high-density-lipoprotein ratio leads to the suggestion that monocyte-to-high-density-lipoprotein ratio might be a reliable parameter in psoriasis during the follow-up. The relationship between the diasease and inflammatory parameters might provide early detection of cardiovascular morbidities in psoriasis patients.

Humans , Male , Female , Adult , Young Adult , Psoriasis/blood , Blood Platelets , C-Reactive Protein/analysis , Lymphocytes , Monocytes , Lipoproteins, HDL/blood , Neutrophils , Platelet Count , Psoriasis/complications , Reference Values , Severity of Illness Index , Biomarkers/blood , Cardiovascular Diseases/etiology , Cardiovascular Diseases/blood , Case-Control Studies , Retrospective Studies , Risk Factors , Analysis of Variance , Statistics, Nonparametric , Leukocyte Count , Middle Aged
Rev. Assoc. Med. Bras. (1992) ; 65(12): 1489-1495, Dec. 2019. tab
Article in English | LILACS | ID: biblio-1057094


SUMMARY OBJECTIVE Evaluate the prevalence of metabolic syndrome (MS) and the main associated maternal factors in women without pre-gestational conditions, in early pregnancy and in the immediate postpartum. METHODS Two hundred pregnant women were evaluated at the 16th week of pregnancy, and 187 were reassessed postpartum. MS was diagnosed according to the criteria by the National Cholesterol Education Program Adult Treatment Panel III. In addition to the diagnostic criteria, anthropometric measures, blood pressure, metabolic profile, and visceral and subcutaneous fat thickness (by ultrasonography) were collected from the pregnant woman. The student's t-test was used to compare the prevalence of MS and its components in the 16th week and in the postpartum. Multiple logistic regression was performed to identify the principal factors associated with the syndrome. RESULTS The prevalence of the MS was 3.0% in early pregnancy and 9.7% postpartum (p=0.01). Following multiple logistic regression, the prepregnancy body mass index (BMI) (p=0.04) and high-density lipoprotein cholesterol (HDL-c) (p=0.02) remained associated with MS at 16 weeks, and triglyceride levels evaluated in postpartum (p<0.001) with MS in postpartum. CONCLUSION The frequency of the MS was high in the immediate postpartum. The factors associated were prepregnancy BMI and HDL-c at the 16th week, as well as triglyceride levels postpartum.

RESUMO OBJETIVO Avaliar a prevalência da SM e os principais fatores maternos associados em mulheres sem doenças pré-gestacionais, no início da gravidez e no pós-parto imediato. MÉTODOS Foram avaliadas 200 mulheres na 16a semana de gravidez, sendo 187 reavaliadas no pós-parto. A SM foi diagnosticada de acordo com os critérios do National Cholesterol Education Program Adult Treatment Panel III. Além dos critérios diagnósticos da síndrome, foram coletadas medidas antropométricas, pressão arterial, perfil metabólico e espessura de gordura visceral e subcutânea (através de ultrassonografia) da gestante. O teste t de Student foi usado para comparar a prevalência de SM e dos seus componentes nos dois momentos. Os modelos de regressão logística múltipla, para investigar os principais fatores associados à síndrome na 16a semana de gestação e no pós-parto. RESULTADOS A prevalência da SM foi de 3,0% no início da gravidez e 9,7% no pós-parto (p=0,01). O índice de massa corporal (IMC) pré-gravídico (p=0,04) e o colesterol lipoproteínas de alta densidade (HDL-c) (p=0,02) permaneceram associados à SM na 16a semana. Após o parto, os níveis de triglicerídeos permaneceram associados à SM no pós-parto (p<0,001). CONCLUSÃO A prevalência da SM foi alta no pós-parto imediato e os fatores associados à síndrome foram IMC pré-gravídico e HDL-c na 16a semana, e níveis triglicerídeos no pós-parto.

Humans , Female , Pregnancy , Adult , Young Adult , Pregnancy Complications/epidemiology , Metabolic Syndrome/epidemiology , Postpartum Period , Time Factors , Brazil/epidemiology , Body Mass Index , Logistic Models , Prevalence , Risk Factors , Analysis of Variance , Gestational Age , Statistics, Nonparametric , Lipoproteins, HDL/blood
Gac. méd. Méx ; 155(5): 453-457, Sep.-Oct. 2019. tab
Article in English | LILACS | ID: biblio-1286542


Introduction: The low-density lipoprotein (LDL)/high-density lipoprotein (HDL) index is a predictive factor for atherosclerosis, which is associated with oxidative modifications. Objective: To assess the association of the index with oxidative stress markers. Methods: 444 subjects were included and were clinically, anthropometrically and biochemically characterized; superoxide dismutase, glutathione peroxidase 3 (GPx3), magnesium and oxidized LDL (oxLDL) index (oxLDL/HDL) were quantified. Results: A decrease of 1.014 units in the LDL/HDL index was associated with a superoxide dismutase increase of 1 unit/mL (p = 0.030), while a decrease of 0.023 units was associated with a GPx3 increase of 1 nmol/min/mL (p < 0.0005). An increase of one unit in the index was associated with an increase of 0.831 in the oxLDL/HDL index (p < 0.05). After controlling for the effect of gender, age, smoking, obesity and insulin resistance, a reduction of 0.001 per index unit was associated with an increase of 1 µg/g of magnesium in the nails (p = 0.020). Conclusions: The LDL/HDL index shows an inverse relationship with the antioxidant status and a direct relationship with oxidation status, regardless of other cardiovascular and oxidative stress risk factors.

Humans , Male , Female , Adult , Superoxide Dismutase/blood , Oxidative Stress , Glutathione Peroxidase/blood , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Insulin Resistance , Smoking , Sex Factors , Cross-Sectional Studies , Age Factors , Magnesium/analysis , Nails/chemistry , Obesity
J. pediatr. (Rio J.) ; 95(4): 428-434, July-Aug. 2019. tab
Article in English | LILACS | ID: biblio-1040335


Abstract Objective: Given the importance of incorporating simple and low-cost tools into the pediatric clinical setting to provide screening for insulin resistance, the present study sought to investigate whether waist-to-height ratio is comparable to biochemical markers for the discrimination of insulin resistance in children and adolescents. Methods: This cross-sectional study involved students from nine public schools. In total, 296 children and adolescents of both sexes, aged 8 -14 years, composed the sample. Waist-to-height ratio, triglycerides/glucose index, and triglycerides-to-HDL-C ratio were determined according to standard protocols. Insulin resistance was defined as homeostatic model assessment for insulin resistance with cut-off point ≥ 3.16. Results: Age, body mass index, frequency of overweight, waist circumference, waist-to-height ratio, insulin, glucose, homeostatic model assessment for insulin resistance, triglycerides, triglycerides/glucose index, and triglycerides-to-HDL-C were higher among insulin resistant boys and girls. Moderate correlation of all indicators (waist-to-height ratio, triglycerides/glucose index, and triglycerides-to-HDL-C ratio) with homeostatic model assessment for insulin resistance was observed for both sexes. The areas under the receiver operational characteristic curves ware similar between waist-to-height ratio and biochemical markers. Conclusion: The indicators provided similar discriminatory power for insulin resistance. However, taking into account the cost-benefit ratio, we suggest that waist-to-height ratio may be a useful tool to provide screening for insulin resistance in pediatric populations.

Resumo Objetivo: Considerando a importância de incorporar ferramentas simples e de baixo custo no cenário clínico-pediátrico para a triagem de resistência à insulina, o presente estudo buscou investigar se a razão cintura/estatura é comparável a marcadores bioquímicos na discriminação de resistência à insulina em crianças e adolescentes. Métodos: Este estudo transversal envolveu estudantes de nove escolas públicas. No total, 296 crianças e adolescentes, de ambos os sexos, com idades entre 8 e 14 anos, compuseram a amostra. A razão cintura/estatura, o índice triglicerídeos/glicose e a razão triglicerídeos/HDL-C foram determinados de acordo com protocolos padrão. A resistência à insulina foi definida por meio do modelo de avaliação homeostática para resistência insulínica, com ponto de corte ≥ 3.16. Resultados: Idade, índice de massa corporal, frequência de excesso de peso, circunferência da cintura, razão cintura/estatura, insulina, glicemia, modelo de avaliação homeostática para resistência insulínica, triglicerídeos, índice triglicerídeos/glicose e razão triglicerídeos/HDL-C foram maiores entre meninos e meninas com resistência à insulina. Também foram observadas, em ambos os sexos, correlações moderadas de todos os indicadores (razão cintura/estatura, índice triglicerídeos/glicose e razão triglicerídeos/HDL-C) com o modelo de avaliação homeostática para resistência à insulina. As áreas sob as curvas ROC foram semelhantes entre a razão cintura/estatura e os marcadores bioquímicos. Conclusão: Os indicadores forneceram poder discriminatório similar para a resistência à insulina. No entanto, levando em conta o custo-benefício, sugerimos que a razão cintura/estatura pode ser uma ferramenta útil para a triagem de resistência à insulina em populações pediátricas.

Humans , Male , Female , Child , Adolescent , Blood Glucose/analysis , Insulin Resistance/physiology , Biomarkers/analysis , Metabolic Syndrome/physiopathology , Waist Circumference/physiology , Waist-Height Ratio , Triglycerides/blood , Brazil , Body Mass Index , Cross-Sectional Studies , Metabolic Syndrome/blood , Overweight/physiopathology , Overweight/blood , Lipoproteins, HDL/blood , Cholesterol, HDL/blood
Arq. bras. oftalmol ; 82(3): 220-224, May-June 2019. tab, graf
Article in English | LILACS | ID: biblio-1001299


ABSTRACT Purpose: Beta-thalassemia minor, a common hereditary blood disorder in Mediterranean countries such as Turkey, is associated with insulin resistance. Insulin resistance, in turn, can be associated with excessively high intraocular pressure and, therefore, intraocular pressure-induced blindness. This study aimed to investigate the intraocular pressure in subjects with beta-thalassemia minor. Methods: We conducted a cross-sectional study comprising of 203 subjects divided into two groups: beta-thalassemia minor (103) and healthy (100).Hemoglobin electrophoresis was performed and complete blood count, blood pressures, serum fasting glucose and insulin levels were measured. All subjects underwent ophthalmological examinations including intraocular pressure measurements. Results: Intraocular pressure in the subjects with beta-thalassemia minor was significantly lower than that in healthy subjects (p=0.007). Additionally, intraocular pressure was inversely correlated with hemoglobin A2 levels (p=0.001, r=-0.320). Serum insulin and systolic blood pressure were significantly higher in subjects with beta-thalassemia minor (p=0.03, p=0.009, respectively). Conclusion: Subjects with beta-thalassemia minor had lower intraocular pressure than healthy controls, suggesting beta-thalassemia minor may actually protect against high intraocular pressure.

RESUMO Objetivo: Beta-talassemia menor é uma doença hereditária comum no sangue em países mediterrâneos como a Turquia e está associada à resistência à insulina. A resistência à insulina por sua vez, pode estar associada à pressão intraocular excessivamente alta e, portanto à cegueira induzida pela pressão intraocular. Este estudo teve como objetivo investigar a pressão intraocular em indivíduos com beta-talassemia menor. Métodos: Foi realizado um estudo transversal compreendendo 203 indivíduos divididos em 2 grupos: beta-talassemia menor (103) e saudável (100). Eletroforese de hemoglobina foi realizada e hemograma completo, pressão arterial, glicemia em jejum e níveis de insulina medidos. Todos os indivíduos foram submetidos foram submetidos a exames oftalmológicos, incluindo medidas de pressão intraocular. Resultados: A pressão intraocular nos indivíduos com beta-talassemia menor foi significativamente menor do que em indivíduos saudáveis (p=0,007). Além disso, a pressão intraocular foi inversamente correlacionada com os níveis de hemoglobina A2 (p=0,001, r=-0,320). Insulina sérica e pressão arterial sistólica foram significativamente maiores em indivíduos com beta-talassemia menor (p=0,03, p=0,009, respectivamente). Conclusão: Os indivíduos com beta-talassemia menor tiveram pressão intraocular menor do que os controles saudáveis, sugerindo que a beta-talassemia menor pode, na verdade, proteger contra a alta pressão intraocular.

Humans , Male , Female , Adult , Middle Aged , beta-Thalassemia/physiopathology , Intraocular Pressure/physiology , Reference Values , Tonometry, Ocular , Triglycerides/blood , Blood Glucose/analysis , Blood Pressure/physiology , Hemoglobin A2/analysis , Insulin Resistance/physiology , Case-Control Studies , Linear Models , Cross-Sectional Studies , beta-Thalassemia/blood , Statistics, Nonparametric , Insulin/blood , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood
Arch. Clin. Psychiatry (Impr.) ; 46(2): 33-39, Mar.-Apr. 2019. tab, graf
Article in English | LILACS | ID: biblio-1011143


Abstract Objective To compare sex difference in metabolic effect of olanzapine versus aripiprazole on schizophrenia. Methods A twelve-week prospective open-label cohort study to compare four subgroups according to first-episode schizophrenia patients' type of drug usage and sex: female aripiprazole (n = 11), male aripiprazole (n = 11), female olanzapine (n = 10), and male olanzapine (n = 11) for body mass index, fasting serum triglyceride, total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and fasting glucose. Results Aripiprazole may be associated with weight gain in female patients with low-baseline weight. Aripiprazole may have an adverse effect of weight and favorable effects of circulating glucose and lipid on female over male schizophrenia patients. The aripiprazole-induced changes in glucose and lipid may be independent of body fat storage, especially for female schizophrenia patients. Olanzapine may have adverse effects of weight, glucose and lipid profiles on female over male schizophrenic patients. Discussion Our findings fill the gap in knowledge and provide a sex-specific guidance to psychiatrist better tailoring treatment to individual sex-differential characteristics and a key clue to understand the sex-differential mechanism of antipsychotics-induced metabolic dysfunction.

Humans , Male , Female , Adult , Middle Aged , Blood Glucose/drug effects , Lipid Metabolism/drug effects , Aripiprazole/adverse effects , /adverse effects , Schizophrenia/drug therapy , Triglycerides/blood , Weight Gain/drug effects , Body Mass Index , Sex Factors , Prospective Studies , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood
Arq. bras. cardiol ; 112(1): 12-17, Jan. 2019. tab, graf
Article in English | LILACS | ID: biblio-973841


Abstract Background: Assessing the monocyte to high-density lipoprotein ratio (MHR) is a new tool for predicting inflamation, which plays a major role in atherosclerosis. Myocardial bridge (MB) is thought to be a benign condition with development of atherosclerosis, particularly at the proximal segment of the brigde. Objective: To evaluate the relationhip between MHR and the presence of MB. Methods: We consecutively scanned patients referred for coronary angiography between January 2013- December 2016, and a total of 160 patients who had a MB and normal coronary artery were enrolled in the study. The patients' angiographic, demographic and clinic characteristics of the patients were reviewed from medical records. Monocytes and HDL-cholesterols were measured via complete blood count. MHR was calculated as the ratio of the absolute monocyte count to the HDL-cholesterol value. MHR values were divided into three tertiles as follows: lower (8.25 ± 1.61), moderate (13.11 ± 1.46), and higher (21.21 ± 4.30) tertile. A p-value of < 0.05 was considered significant. Results: MHR was significantly higher in the MB group compared to the control group with normal coronary arteries. We found the frequency of MB (p = 0.002) to increase as the MHR tertiles rose. The Monocyte-HDL ratio with a cut-point of 13.35 had 59% sensitivity and 65.0% specificity (ROC area under curve: 0.687, 95% CI: 0.606-0.769, p < 0.001) in accurately predicting a MB diagnosis. In the multivariate analysis, MHR (p = 0.013) was found to be a significant independent predictor of the presence of MB, after adjusting for other risk factors. Conclusion: The present study revealed a significant correlation between MHR and MB.

Resumo Fundamento: A avaliação da razão de monócitos para lipoproteínas de alta densidade (MHR, sigla em inglês) é uma nova ferramenta para se prever o processo inflamatório, o qual desempenha um papel importante na aterosclerose. A ponte miocárdica (PM) é considerada uma condição benigna com desenvolvimento de arteriosclerose, particularmente no segmento proximal da ponte. Objetivo: Avaliar a relação entre a MHR e a presença de PM. Métodos: Examinamos concecutivamente pacientes encaminhados para angiografia coronariana entre janeiro de 2013 e dezembro de 2016, e um total de 160 pacientes, uma parcela dos quais com PM, e outra com artérias coronárias normais, foram incluídos no estudo. As características angiográficas, demográficas e clínicas dos pacientes foram revisadas a partir de registros médicos. Monócitos e colesteróis HDL foram medidos através de hemograma completo. A MHR foi calculada como a razão entre a contagem absoluta de monócitos e o valor do colesterol HDL. Os valores de MHR foram divididos em três tercis, da seguinte forma: tercil inferior (8,25 ± 1,61); tercil moderado (13,11 ± 1,46); e tercil superior (21,21 ± 4,30). Considerou-se significativo um valor de p < 0,05. Resultados: A MHR foi significativamente maior no grupo com PM, em comparação com grupo controle com artérias coronárias normais. Verificamos que a prevalência de PM (p=0,002) aumentou à medida que se elevavam os tercis de MHR. A razão monócitos-colesterol HDL com ponto de corte de 13,35 apresentou sensibilidade de 59% e especificidade de 65,0% (área ROC sob a curva: 0,687, IC95%: 0,606-0,769, p < 0,001) na predição acurada do diagnóstico de PM. Na análise multivariada, a MHR (p = 0,013) mostrou-se um preditor independente significativo da presença de PM, após ajustes para outros fatores de risco. Conclusão: O presente estudo revelou uma correlação significativa entre MHR e PM.

Humans , Male , Female , Adult , Middle Aged , Monocytes , Myocardial Bridging/blood , Lipoproteins, HDL/blood , Reference Values , Blood Cell Count , Case-Control Studies , Multivariate Analysis , Regression Analysis , Risk Factors , Sensitivity and Specificity , Coronary Angiography , Statistics, Nonparametric , Atherosclerosis/blood , Cholesterol, LDL/blood
Cad. Saúde Pública (Online) ; 35(12): e00200418, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055591


Abstract: We sought (i) to evaluate the associations of cardiorespiratory fitness (CRF), muscular fitness (MF) and Southern European Atlantic Diet (SEADiet) with atherogenic index of plasma (AIP) and (ii) to investigate de combined association of MF, CRF and SEADiet on AIP in adolescents. A cross-sectional school-based study was conducted on 493 adolescents (285 girls and 208 boys) aged 15-18 years, from the Portuguese Azorean Archipelago. CRF was measured by shuttle run test and MF by curl up and push up tests. Adherence to SEADiet was assessed with a semi-quantitative food frequency questionnaire (FFQ). The AIP was estimated as log (TG/HDL-C). Measures of pubertal stage and socioeconomic status were assessed. Linear regression showed a significant inverse association between MF (standardized β = -0.165; p < 0.001), CRF (standardized β = -0.081; p < 0.030) and SEADiet (standardized β = -0.081; p < 0.045) with AIP, after adjustments for age, sex, pubertal stage and parental education. Furthermore, participants classified with an optimal as well as those with low adherence to a SEADiet but with LowMF/LowCRF had on average the highest AIP (F(7.482) = 3.270; p = 0.002). Moreover, optimal SEADiet with HighMF/HighCRF group showed the lowest AIP when compared with those with low adherence to a SEADiet with HighMF/HighCRF group (p = 0.03). AIP is inversely associated with MF, CRF and SEADiet. The low MF combined with a low CRF levels seems to overcome the potential healthy effect of having an optimal adherence to the SEADiet on AIP. However, an optimal adherence to SEADiet seems to improve the AIP in those adolescents with high fitness levels.

Resumo: O estudo teve como objetivos: (i) avaliar as associações entre a aptidão cardiorrespiratória (ACR), aptidão muscular (AM) e Dieta Atlântica do Sul da Europa (SEADiet) e o índice aterogênico do plasma (IAP) e (ii) investigar a associação combinada entre AM, ACR e SEADiet e IAP em adolescentes. Foi realizado um estudo transversal de base escolar em uma amostra de 493 adolescentes (285 do sexo feminino), entre 15 e 18 anos de idade, nos Arquipélago dos Açores, Portugal. A ACR foi medida pelo teste aeróbico de corrida de vai-e-vem e a AM pelos testes de abdominal modificado e de flexão. A adesão ao SEADiet foi avaliada através de um questionário semi-quantitativo de frequência alimentar. O IAP foi calculado como log(TG/HDL-C). Foram avaliadas as medidas de estágio de puberdade e de nível socioeconômico. A regressão linear revelou uma associação inversa significativa entre AM (β padronizado = -0,165; p < 0,001), ACR (β padronizado = -0,081; p < 0,030) e SEADiet (β padronizado = -0,081; p < 0,045) e IAP, depois de ajustar para idade, sexo, estágio de puberdade e escolaridade dos pais. Além disso, os participantes classificados como adesão excelente ou baixa ao padrão alimentar SEADiet mas com BaixaAM/BaixaACR, tiveram, em média, os maiores níveis de IAP (F(7,482) = 3,270; p = 0,002). O grupo com adesão excelente ao SEADiet e AltaAM/AltaACR mostrou IAP mais baixo, comparado com o grupo com baixa adesão ao padrão alimentar SEADiet e AltaAM/AltaACR (p = 0,03). O IAP está associado inversamente com AM, ACR e SEADiet. Os níveis baixos de AM e ACR parecem contrabalançar o efeito saudável potencial da adesão excelente ao padrão alimentar SEADiet sobre o IAP. Entretanto, a adesão excelente ao SEADiet parece melhorar o IAP nos adolescentes com níveis altos de aptidão física.

Resumen: Nuestro objetivo (i) fue evaluar las asociaciones de las condiciones cardiorrespiratorias (CRF), musculares (MF) y de la Dieta Atlántica del Sur de Europa (SEADiet), con el índice aterogénico de plasma (AIP), así como (ii) investigar la asociación combinada de MF, CRF y SEADiet en el AIP en adolescentes. Se trata de un estudio transversal, basado escolares, que se dirigió a 493 adolescentes (285 niñas), con edades comprendidas entre los 15 a 18 años, procedentes del archipiélago portugués de las Azores. Las CRF se midieron mediante una prueba de sprint y las MF mediante pruebas con flexiones y abdominales. La adherencia a la SEADiet se evaluó con un cuestionario semicuantitativo sobre la frecuencia de las comidas. El AIP se calculó como log (TG/HDL-C). Se evaluaron las medidas de la fase puberal y el estatus socioeconómico. La regresión lineal mostró una asociación significativa inversa entre MF (β estandarizado = -0,165; p < 0,001), CRF (β estandarizado = -0,081; p < 0,030) y SEADiet (β estandarizado = -0,081; p < 0,045) con el AIP, tras los ajustes por edad, sexo, fase puberal y educación parental. Asimismo, los participantes clasificados con una adherencia muy buena, así como los que tuvieron una baja adherencia a la SEADiet, pero con BajasMF/BajasCRF contaron con el promedio más alto en el AIP (F(7.482) = 3.270; p = 0,002). Además, el grupo con una muy buena SEADiet con AltasMF/AltasCRF mostró el más bajo AIP, cuando se compara con el grupo que tenía una baja adherencia a la SEADiet con AltasMF/AltasCRF (p = 0.03). El AIP está inversamente asociado con MF, CRF y SEADiet. La baja MF, combinada con los niveles bajos de CRF, parece superar el efecto potencial saludable de contar con una muy buena adherencia a la SEADiet en el AIP. No obstante, una muy buena adherencia a la SEADiet parece mejorar el AIP en aquellos adolescentes con altos niveles de condición física.

Humans , Male , Female , Adolescent , Triglycerides/blood , Physical Fitness/physiology , Diet/methods , Cardiorespiratory Fitness/physiology , Lipoproteins, HDL/blood , Brazil , Cross-Sectional Studies , Adolescent Behavior
Braz. j. med. biol. res ; 52(7): e8429, 2019. graf
Article in English | LILACS | ID: biblio-1011597


The present study aimed to analyze age-related changes to motor coordination, balance, spinal cord oxidative biomarkers in 3-, 6-, 18-, 24-, and 30-month-old rats. The effects of low-intensity exercise on these parameters were also analyzed in 6-, 18-, and 24-month-old rats. Body weight, blood glucose, total cholesterol, and high-density lipoprotein (HDL) cholesterol were assessed for all rats. The soleus muscle weight/body weight ratio was used to estimate skeletal muscle mass loss. Body weight increased until 24 months; only 30-month-old rats exhibited decreased blood glucose and increased total cholesterol and HDL cholesterol. The soleus muscle weight/body weight ratio increased until 18 months, followed by a small decrease in old rats. Exercise did not change any of these parameters. Stride length and step length increased from adult to middle age, but decreased at old age. Stride width increased while the sciatic functional index decreased in old rats. Performance in the balance beam test declined with age. While gait did not change, balance improved after exercise. Aging increased superoxide anion generation, hydrogen peroxide levels, total antioxidant capacity, and superoxide dismutase activity while total thiol decreased and lipid hydroperoxides did not change. Exercise did not significantly change this scenario. Thus, aging increased oxidative stress in the spinal cord, which may be associated with age-induced changes in gait and balance. Regular low-intensity exercise is a good alternative for improving age-induced changes in balance, while beneficial effects on gait and spinal cord oxidative biomarkers cannot be ruled out because of the small number of rats investigated (n=5 or 6/group).

Animals , Male , Rats , Physical Conditioning, Animal/physiology , Biomarkers/blood , Age Factors , Oxidative Stress/physiology , Postural Balance/physiology , Gait/physiology , Spinal Cord/physiology , Spinal Cord/metabolism , Blood Glucose/analysis , Body Weight/physiology , Biomarkers/metabolism , Cholesterol/blood , Rats, Wistar , Lipoproteins, HDL/blood
Acta cir. bras ; 33(6): 524-532, June 2018. graf
Article in English | LILACS | ID: biblio-949352


Abstract Purpose: To evaluate in vivo animal model of cardiac ischemia/reperfusion the cardioprotective activity of pancreatic lipase inhibitor of the orlistat. Methods: Adult male Wistar rats were anesthetized, placed on mechanical ventilation and underwent surgery to induce cardiac I/R by obstructing left descending coronary artery followed by reperfusion to evaluation of ventricular arrhythmias (VA), atrioventricular block (AVB) and lethality (LET) with pancreatic lipase inhibitor orlistat (ORL). At the end of reperfusion, blood samples were collected for determination of triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine kinase (CK), and creatine kinase-MB (CK-MB). Results: Treatment with ORL has been able to decrease the incidence of VA, AVB and LET. Besides that, treatment with ORL reduced serum concentrations of CK and LDL, but did not alter the levels of serum concentration of TG, VLDL and HDL. Conclusion: The reduction of ventricular arrhythmias, atrioventricular block, and lethality and serum levels of creatine kinase produced by treatment with orlistat in animal model of cardiac isquemia/reperfusion injury suggest that ORL could be used as an efficient cardioprotective therapeutic strategy to attenuate myocardial damage related to acute myocardial infarction.

Animals , Male , Cardiotonic Agents/pharmacology , Myocardial Reperfusion Injury/prevention & control , Lactones/pharmacology , Myocardial Infarction/prevention & control , Arrhythmias, Cardiac/prevention & control , Triglycerides/blood , Myocardial Reperfusion Injury/blood , Random Allocation , Reproducibility of Results , Risk Factors , Treatment Outcome , Rats, Wistar , Creatine Kinase/blood , Electrocardiography , Atrioventricular Block/prevention & control , L-Lactate Dehydrogenase/blood , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Lipoproteins, VLDL/blood , Myocardial Infarction/blood
Rev. peru. med. exp. salud publica ; 35(1): 15-24, ene.-mar. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-961856


RESUMEN Objetivos. Evaluar los niveles de lipoproteínas de alta densidad (HDL) y citoquinas proinflamatorias en pacientes con dengue clasificados según la gravedad del cuadro clínico. Materiales y métodos. Se realizó un estudio descriptivo, incluyendo 70 individuos con dengue, clasificados como: sin signos de alarma (DSSA), con signos de alarma (DCSA) y dengue grave (DG); 15 donantes sanos fueron incluidos como controles (CS). Los niveles séricos de IL-1β e IL-6 fueron cuantificados por ELISA, y los de HDL en suero por ensayo colorimétrico; además se determinó el recuento de plaquetas y el porcentaje de hematocrito. Resultados. Los niveles de IL-1β e IL-6 se encontraron aumentados en los pacientes con DENV respecto a los controles sanos. Dicho aumento fue más evidente en los pacientes con dengue, de acuerdo con la gravedad del cuadro clínico. Los niveles de HDL se hallaron disminuidos en los pacientes con dengue comparados con los controles sanos. Los análisis de correlación mostraron asociaciones estadísticamente significativas entre HDL y el número de plaquetas; así como entre el porcentaje de hematocrito y los niveles de IL-1β. Conclusiones. El aumento de IL-1β e IL-6 y la disminución de HDL en los estadios clínicos más graves sugieren que ambos factores están implicados en el desarrollo de la patogénesis y severidad. Las correlaciones permiten observar una posible asociación entre HDL y el número de plaquetas que permite dar una aproximación al posible papel de las HDL en la patogénesis del dengue, pero aún es necesario realizar estudios adicionales que permitan elucidar su importancia en el curso de la infección por DENV.

ABSTRACT Objectives. To assess High-Density Lipoprotein (HDL) and pro-inflammatory cytokine levels in patients with dengue classified according to the severity of the clinical presentation. Materials and Methods. A descriptive study was carried out with 70 individuals with dengue, classified as no alarm signs (DSSA), with signs of alarm (DCSA), and severe dengue (DG); 15 healthy donors were included as controls (CS). The serum levels of IL-1β and IL-6 were quantified through ELISA, and the HDL levels through serum by a colorimetric test. In addition, the platelet count and the hematocrit percentage were determined. Results. Levels of IL-1β and IL-6 were found to be increased in patients with DENV vs. the healthy controls. Said increase was more evident in patients with dengue and in line with the severity of the clinical presentation. The HDL levels were found to be decreased in patients with dengue versus healthy controls. The correlation analysis showed statistically significant associations between HDL and the platelet number, as well as between the hematocrit percentage and the levels of IL-1β. Conclusions. The increased IL-1β and IL-6 and the decreased HDL levels in the most severe clinical stages suggest that both factors are involved in the development of the pathogenesis and severity. The correlations allow the observation of a potential association between HDL and platelet count, which allows us to provide an approach to the possible role of HDL in the pathogenesis of dengue, but it is still necessary to perform additional studies that will allow to state their importance in the course of the DENV infection.

Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Young Adult , Interleukin-6/blood , Dengue/blood , Interleukin-1beta/blood , Lipoproteins, HDL/blood , Severity of Illness Index , Cross-Sectional Studies , Retrospective Studies
Biol. Res ; 51: 34, 2018. tab, graf
Article in English | LILACS | ID: biblio-983938


BACKGROUND AND AIMS: Atherosclerotic cardiovascular disease is highly prevalent and its underlying pathogenesis involves dyslipidemia including pro-atherogenic high density lipoprotein (HDL) remodeling. Vitamins C and E have been proposed as atheroprotective agents for cardiovascular disease management. However, their effects and benefits on high density lipoprotein function and remodeling are unknown. In this study, we evaluated the role of vitamin C and E on non HDL lipoproteins as well as HDL function and remodeling, along with their effects on inflammation/ oxidation biomarkers and atherosclerosis in atherogenic diet-fed SR-B1 KO/ApoER61h/h mice. METHODS AND RESULTS: Mice were pre-treated for 5 weeks before and during atherogenic diet feeding with vitamin C and E added to water and diet, respectively. Compared to a control group, combined vitamin C and E administration reduced serum total cholesterol and triglyceride levels by decreasing apo B-48-containing lipoproteins, remodeled HDL particles by reducing phospholipid as well as increasing PON1 and apo D content, and diminished PLTP activity and levels. Vitamin supplementation improved HDL antioxidant function and lowered serum TNF-α levels. Vitamin C and E combination attenuated atherogenesis and increased lifespan in atherogenic diet-fed SR-B1 KO/ApoER61h/h mice. CONCLUSIONS: Vitamin C and E administration showed significant lipid metabolism regulating effects, including HDL remodeling and decreased levels of apoB-containing lipoproteins, in mice. In addition, this vitamin supplementation generated a cardioprotective effect in a murine model of severe and lethal atherosclerotic ischemic heart disease.

Animals , Male , Female , Ascorbic Acid/pharmacology , Vitamin E/pharmacology , Myocardial Ischemia/prevention & control , Apolipoprotein B-48/drug effects , Hyperlipidemias/prevention & control , Lipoproteins, HDL/drug effects , Antioxidants/pharmacology , Reference Values , Coronary Artery Disease/prevention & control , Coronary Artery Disease/blood , Enzyme-Linked Immunosorbent Assay , Cardiotonic Agents/pharmacology , Immunoblotting , Reproducibility of Results , Cytokines/blood , Treatment Outcome , Myocardial Ischemia/blood , Dietary Supplements , Phospholipid Transfer Proteins/blood , Diet, Atherogenic , Scavenger Receptors, Class B/drug effects , Scavenger Receptors, Class B/blood , Lipid Metabolism/drug effects , Apolipoprotein B-48/blood , Hyperlipidemias/blood , Lipoproteins, HDL/blood , Mice, Inbred C57BL
Arch. endocrinol. metab. (Online) ; 61(3): 282-287, May-June 2017. tab, graf
Article in English | LILACS | ID: biblio-887565


ABSTRACT Background Cardiometabolic risk is high in patients with hypogonadism. Visceral adiposity index (VAI) and triglyceride/high-density lipoprotein cholesterol (TG/HDL-C) ratio are the practical markers of atherosclerosis and insulin resistance and independent predictors of cardiaovascular risk. To date, no study has evaluated VAI levels and TG/HDL-C ratio in hypogonadism. Subjects and methods A total of 112 patients with congenital hypogonadotrophic hypogonadism (CHH) (mean age, 21.7 ± 2.06 years) and 124 healthy subjects (mean age, 21.5 ± 1.27 years) were enrolled. The demographic parameters, VAI, TG/HDL-C ratio, asymmetric dimethylarginine (ADMA), high-sensitivity C-reactive protein (hs-CRP), and homeostatic model assessment of insulin resistance (HOMA-IR) levels were measured for all participants. Results The patients had higher total cholesterol (p = 0.04), waist circumference, triglycerides, insulin, and HOMA-IR levels (p = 0.001 for all) than the healthy subjects. VAI and ADMA and TG/HDL-C levels were also higher in patients than in healthy subjects (p < 0.001 for all). VAI was weakly correlated with ADMA (r = 0.27, p = 0.015), HOMA-IR (r = 0.22, p = 0.006), hs-CRP (r = 0.19, p = 0.04), and total testosterone (r = −0.21, p = 0.009) levels, whereas TG/HDL-C ratio was weakly correlated weakly with ADMA (r = 0.30, p = 0.003), HOMA-IR (r = 0.22, p = 0.006), and total testosterone (r = −0.16, p = 0.03) levels. Neither VAI nor TG/HDL-C ratio determined ADMA, HOMA-IR, and hs-CRP levels. Conclusions The results of this study demonstrate that patients with hypogonadism have elevated VAI and TG/HDL-C ratio. These values are significantly correlated with the surrogate markers of endothelial dysfunction, inflammation, and insulin resistance. However, the predictive roles of VAI and TG/HDL-C ratio are not significant. Prospective follow-up studies are warranted to clarify the role of VAI and TG/HDL-C ratio in predicting cardiometabolic risk in patients with hypogonadism.

Humans , Male , Young Adult , Triglycerides/blood , Intra-Abdominal Fat/metabolism , Adiposity/physiology , Hypogonadism/metabolism , Lipoproteins, HDL/blood , Arginine/analogs & derivatives , Arginine/blood , Algorithms , C-Reactive Protein/analysis , Insulin Resistance/physiology , Endothelium, Vascular/physiopathology , Biomarkers/blood , Cardiovascular Diseases/etiology , Cardiovascular Diseases/metabolism , Case-Control Studies , Predictive Value of Tests , Hypogonadism/complications
Einstein (Säo Paulo) ; 15(2): 136-140, Apr.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-891366


ABSTRACT Objective To investigate the inter-relation between high sensitivity C-reactive protein and glycated hemoglobin in prediction of risk of obstructive sleep apnea. Methods We included all individuals participating in a check-up program at the Preventive Medicine Center of Hospital Israelita Albert Einstein in 2014. The Berlin questionnaire for risk of obstructive sleep apnea was used, and the high sensitivity C-reactive protein and glycated hemoglobin levels were evaluated. Results The sample included 7,115 participants (age 43.4±9.6 years, 24.4% women). The Berlin questionnaire showed changes in 434 (6.1%) individuals. This finding was associated with high sensitivity C-reactive protein and glycated hemoglobin levels (p<0.001). However, only the association between the Berlin questionnaire result and glycated hemoglobin remained significant in the adjusted multivariate analysis, for the traditional risk factors and for an additional model, including high-density lipoprotein cholesterol and triglycerides. Conclusion The glycated hemoglobin, even below the threshold for diagnosis of diabetes, is independently associated with obstructive sleep apnea syndrome, even after adjustment for obesity and C-reactive protein. These findings suggest a possible pathophysiological link between changes in insulin resistance and obstructive sleep apnea syndrome, independently from obesity or low-grade inflammation.

RESUMO Objetivo Investigar a inter-relação entre proteína C-reativa de alta sensibilidade e hemoglobina glicada na predição do risco de apneia obstrutiva do sono. Métodos Foram incluídos todos os indivíduos participantes do programa de check-up do Centro de Medicina Preventiva Hospital Israelita Albert Einstein em 2014. Foi aplicado o questionário de Berlin sobre risco de apneia do sono, e avaliadas as dosagens de hemoglobina glicada e proteína C-reativa de alta sensibilidade. Resultados Foram incluídos 7.115 participantes (idade 43,4±9,6 anos, 24,4% mulheres). A prevalência de alteração no questionário de Berlin foi de 434 (6,1%). A alteração do questionário de Berlin associou-se positivamente aos resultados da proteína C-reativa de alta sensibilidade e da hemoglobina glicada (p<0,001). No entanto, apenas a associação entre o resultado do questionário de Berlin e a hemoglobina glicada permaneceu significativa na análise multivariada ajustada tanto para fatores de risco tradicionais quanto para um modelo adicional, que incluiu também lipoproteína de alta densidade-colesterol (HDL-c) e triglicérides. Conclusão A hemoglobina glicada, mesmo em valores abaixo do critério diagnóstico para diabetes mellitus, está associada de forma independente ao risco para síndrome da apneia obstrutiva do sono, mesmo após ajuste para obesidade e proteína C-reativa. Estes achados sugerem possível ligação fisiopatológica entre alterações na resistência insulínica e a síndrome da apneia obstrutiva do sono, que independe da obesidade ou inflamação de baixo grau.

Humans , Male , Female , Adult , Middle Aged , C-Reactive Protein/analysis , Glycated Hemoglobin/analysis , Sleep Apnea, Obstructive/blood , Triglycerides/blood , Brazil/epidemiology , Prevalence , Cross-Sectional Studies , Surveys and Questionnaires , Risk Factors , Sleep Apnea, Obstructive/diagnosis , Sleep Apnea, Obstructive/epidemiology , Inflammation/blood , Lipoproteins, HDL/blood , Obesity/blood
J. pediatr. (Rio J.) ; 92(4): 388-393, July-Aug. 2016. tab
Article in English | LILACS | ID: lil-792580


Abstract Objective Verify the association between metabolic risk profile in students with different levels of cardiorespiratory fitness and body mass index, as well as the nutritional status of their parents. Methods A cross-sectional study comprising 1.254 schoolchildren aged between seven and 17 years. The metabolic risk profile was calculated by summing the standardized values of high density lipoproteins and low density lipoproteins, triglycerides, glucose and systolic blood pressure. The parents’ nutritional status was evaluated by self-reported weight and height data, for body mass index calculating. The body mass index of schoolchildren was classified as underweight/normal weight and overweight/obesity. The cardiorespiratory fitness was assessed by 9-minute running/walk test, being categorized as fit (good levels) and unfit (low levels). Data were analyzed using prevalence ratio values (PR). Results The data indicates a higher occurrence of developing metabolic risk in schoolchildren whose mother is obese (PR: 1.50; 95% CI: 1.01, 2.23), and even higher for those whose father and mother are obese (PR: 2, 79, 95% CI: 1.41; 5.51). Students who have low levels of cardiorespiratory fitness and overweight/obesity have higher occurrence of presenting metabolic risk profile (PR: 5.25; 95% CI: 3.31; 8.16). Conclusion the occurrence of developing metabolic risk in schoolchildren increase when they have low levels of cardiorespiratory fitness and overweight/obesity, and the presence of parental obesity.

Resumo Objetivo Verificar se há associação entre o perfil de risco metabólico em escolares com diferentes níveis de aptidão cardiorrespiratória e índice de massa corporal, bem como com o perfil nutricional de seus pais. Métodos Estudo transversal constituído por 1.254 escolares com idade entre sete e 17 anos. O perfil de risco metabólico foi calculado por meio da soma dos valores estandardizados de lipoproteína de alta densidade e lipoproteína de baixa densidade, triglicerídeos, glicose e pressão arterial sistólica. O perfil nutricional dos pais foi avaliado pelos dados autorreferidos de peso e estatura, calculando-se posteriormente o índice de massa corporal. O índice de massa corporal do escolar foi classificado em baixo peso/peso normal e sobrepeso/obesidade. A aptidão cardiorrespiratória foi avaliada através do teste de corrida/caminhada de 9 minutos, sendo categorizada em apto (bons níveis) e inapto (baixos níveis). Os dados foram analisados através dos valores de razão de prevalência (RP). Resultados Os dados apontam maior ocorrência de desenvolvimento de risco metabólico em escolares que apresentam mãe com obesidade (RP: 1,50; IC 95%: 1,01; 2,23) e, maior ainda, em escolares que possuem pai e mãe obesos (RP: 2,79; IC 95%: 1,41; 5,51). Escolares que apresentam baixos níveis de aptidão cardiorrespiratória e sobrepeso/obesidade possuem maior ocorrência de perfil metabólico de risco (RP: 5,25; IC 95%: 3,31; 8,16). Conclusões a ocorrência de desenvolvimento de risco metabólico em escolares aumentam quando estes apresentam baixos níveis de aptidão cardiorrespiratória e sobrepeso/obesidade, assim como na presença de obesidade dos pais.

Humans , Male , Female , Child , Adolescent , Nutritional Status/physiology , Metabolic Syndrome/etiology , Cardiorespiratory Fitness/physiology , Obesity/complications , Parents , Rural Population , Triglycerides/blood , Urban Population , Blood Glucose/analysis , Blood Pressure/physiology , Brazil , Body Mass Index , Sex Factors , Cross-Sectional Studies , Risk Factors , Risk Assessment , Metabolic Syndrome/physiopathology , Exercise Test , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Obesity/physiopathology
Arq. bras. cardiol ; 107(1): 10-19, July 2016. tab, graf
Article in English | LILACS | ID: lil-792494


Abstract Background: Regular physical activity (PA) induces desirable changes in plasma levels of high- and low-density lipoproteins (HDL and LDL, respectively) and triglycerides (TG), important risk factors for cardiometabolic diseases. However, doubts whether intensity and duration have equivalent benefits remain. Objective: To assess the association of PA intensity and duration with HDL, LDL and TG levels. Methods: Cross-sectional study with 12,688 participants from the Brazilian Longitudinal Study of Adult Health (ELSA-Brasil) baseline, who were not on lipid-lowering medication. After adjustment for important covariates, multiple linear regression was used to assess the association of PA intensity and duration with HDL, LDL and TG (natural logarithm) levels. Results: Both moderate and vigorous PA and PA practice ≥ 150 min/week were significantly associated with higher HDL and lower TG levels. Vigorous PA was associated with lower LDL only on univariate analysis. After adjustments, moderate and vigorous PA increased mean HDL level by 0.89 mg/dL and 1.71 mg/dL, respectively, and reduced TG geometric mean by 0.98 mg/dL and 0.93 mg/dL, respectively. PA practice ≥ 150 min/week increased mean HDL level by 1.05 mg/dL, and decreased TG geometric mean by 0.98 mg/dL. Conclusion: Our findings reinforce the benefits of both PA parameters studied on HDL and TG levels, with a slight advantage for vigorous PA as compared to the recommendation based only on PA duration.

Resumo Fundamento: A prática regular de atividade física (AF) induz alterações desejáveis nos níveis das lipoproteínas de alta densidade (HDL) e de baixa densidade (LDL) e dos triglicérides (TG), importantes fatores de risco cardiometabólico, mas persistem dúvidas se intensidade e duração da AF têm benefícios equivalentes. Objetivo: Investigar a associação da intensidade e da duração da AF com os níveis de HDL, LDL e TG. Métodos: Estudo transversal com 12.688 participantes da linha de base do Estudo Longitudinal da Saúde do Adulto que não usavam medicação para controle de lipídeos. Regressão linear múltipla foi usada para avaliar a associação, após ajustes por fatores sociodemográficos e de saúde, entre a intensidade e a duração da AF e os níveis de HDL, LDL e TG (logaritmo natural). Resultados: AF moderada e vigorosa bem como a prática de AF ≥ 150 min/semana foram associadas a maiores níveis de HDL. Maior intensidade de AF e AF ≥ 150 min/semana foram associadas a menores níveis de TG. Após ajustes, AF moderada e AF vigorosa aumentaram a média de HDL em 0,89 mg/dL e 1,71 mg/dL, respectivamente, e reduziram a média geométrica de TG em 0,98 mg/dL e 0,93 mg/dL, respectivamente. AF ≥ 150 min/semana aumentou a média de HDL em 1,05 mg/dL e reduziu a média geométrica de TG em 0,98 mg/dL. Conclusão: Nossos resultados reforçam os benefícios da AF sobre níveis de HDL e TG, sugerindo vantagem para a intensidade vigorosa quando comparada à recomendação baseada apenas na duração da AF.

Humans , Male , Female , Adult , Middle Aged , Aged , Triglycerides/blood , Exercise/physiology , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Reference Values , Socioeconomic Factors , Time Factors , Brazil/epidemiology , Cross-Sectional Studies , Risk Factors , Longitudinal Studies , Sex Distribution , Age Distribution , Statistics, Nonparametric , Dyslipidemias/epidemiology
Sâo Paulo med. j ; 134(3): 234-239, tab
Article in English | LILACS | ID: lil-785803


ABSTRACT: CONTEXT AND OBJECTIVE: Red grape seed extract (RGSE) contains oligomeric proanthocyanidin complexes as a class of flavonoids. These compounds are potent antioxidants and exert many health-promoting effects. This study aimed to determine the effects of RGSE on serum levels of triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoprotein AI (apo-AI) levels and paraoxonase (PON) activity in patients with mild to moderate hyperlipidemia (MMH). DESIGN AND SETTINGS: A randomized double-blind placebo-controlled clinical trial was conducted at Shahid-Modarres Hospital (Tehran, Iran) and Tabriz University of Medical Sciences. Seventy MMH patients were randomly assigned to receive treatment (200 mg/day of RGSE) or placebo for eight weeks. RESULTS: Significant elevation in serum levels of apo-AI (P = 0.001), HDL-C (P = 0.001) and PON activity (P = 0.001) and marked decreases in concentrations of TC (P = 0.015), TG (P = 0.011) and LDL-C (P = 0.014) were found in the cases. PON activity was significantly correlated with apo-AI (r = 0.270; P < 0.01) and HDL-C (r = 0.45; P < 0.001). Significant differences between the RGSE and control groups (before and after treatment) for TC (P = 0.001), TG (P = 0.001), PON (P = 0.03), apo-AI (P = 0.001) and LDL-C (P = 0.002) were seen. CONCLUSION: It is possible that RGSE increases PON activity mostly through increasing HDL-C and apo-AI levels in MMH patients. It may thus have potential beneficial effects in preventing oxidative stress and atherosclerosis in these patients.

RESUMO: CONTEXTO E OBJETIVO: Extrato de semente de uva vermelha (RGSE) contém complexos de proantocianidinas oligoméricas como classe de flavonoides. Estes compostos são antioxidantes potentes e exercem muitos efeitos de promoção da saúde. Este estudo visou determinar os efeitos de RGSE nos níveis séricos de triglicérides (TG), colesterol total (TC), colesterol de lipoproteína alta-densidade (HDL-C), colesterol de lipoproteína baixa-densidade (LDL-C), apolipoproteína AI (apo-AI) e atividade de paraoxonase (PON) em pacientes com hiperlipidemia leve a moderada (MMH). DESENHO E LOCAL: Estudo clínico randomizado duplo-cego controlado com placebo, realizado no Hospital Shahid-Modarres (Teerã, Irã) e na Universidade de Ciências Médicas de Tabriz. Setenta pacientes com MMH foram aleatoriamente designados para receber tratamento (200 mg/dia de RGSE) ou placebo durante oito semanas. RESULTADOS: Elevação significativa nos níveis séricos de apo-AI (P = 0,001), HDL-C (P = 0,001) e atividade de PON (P = 0,001) e diminuição marcada nas concentrações de TC (P = 0,015), TG (P = 0,011) e LDL-C (P = 0,014) foram encontradas nos casos. Atividade de PON mostrou correlação significativa com apo-AI (r = 0,270; P < 0,01) e HDL-C (r = 0,45; P < 0,001). Diferenças significativas entre os grupos RGSE e controle (antes e após tratamento) para TC (P = 0,001), TG (P = 0,001), PON (P = 0,03), apo-AI (P = 0,001) e LDL-C (P = 0,002) foram observadas. CONCLUSÃO: É possível que RGSE aumente atividade de PON principalmente através da elevação dos níveis de HDL-C e apo-AI em pacientes MMH. Ele pode, assim, ter efeitos benéficos potenciais na prevenção de estresse oxidativo e aterosclerose nesses pacientes.

Humans , Male , Female , Adult , Middle Aged , Young Adult , Aryldialkylphosphatase/blood , Grape Seed Extract/therapeutic use , Hyperlipidemias/drug therapy , Antioxidants/therapeutic use , Placebos , Triglycerides/blood , Cholesterol/blood , Double-Blind Method , Lipoproteins, HDL/blood , Lipoproteins, LDL/blood , Phytotherapy
Braz. j. med. biol. res ; 48(11): 973-982, Nov. 2015. tab, graf
Article in English | LILACS | ID: lil-762908


Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to proteome-wide expression profiling by two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight tandem mass spectrometry. Proteomic results were validated by immunoblotting and bioinformatically analyzed using MetaCore. From a total of 32 proteins identified with 1.5-fold changes in expression compared with healthy controls, 16 proteins were perturbed in BD independent of mood state, while 16 proteins were specifically associated with particular BD mood states. Two mood-independent differential proteins, apolipoprotein (Apo) A1 and Apo L1, suggest that BD pathophysiology may be associated with early perturbations in lipid metabolism. Moreover, down-regulation of one mood-dependent protein, carbonic anhydrase 1 (CA-1), suggests it may be involved in the pathophysiology of depressive episodes in BD. Thus, BD pathophysiology may be associated with early perturbations in lipid metabolism that are independent of mood state, while CA-1 may be involved in the pathophysiology of depressive episodes.

Adolescent , Adult , Female , Humans , Male , Young Adult , Apolipoprotein A-I/blood , Apolipoproteins/blood , Bipolar Disorder/blood , Carbonic Anhydrase I/blood , Lipid Metabolism Disorders/metabolism , Lipoproteins, HDL/blood , Proteomics , Bipolar Disorder/complications , Bipolar Disorder/diagnosis , Databases, Protein , Diagnosis, Differential , Disease Progression , Down-Regulation , Depressive Disorder, Major/diagnosis , Electrophoresis, Gel, Two-Dimensional , Immunoblotting , Immunoprecipitation , Lipid Metabolism Disorders/complications , Mass Spectrometry/methods
An. bras. dermatol ; 90(2): 265-267, Mar-Apr/2015. graf
Article in English | LILACS | ID: lil-741067


A sixty-one year old white female was referred to the Dermatology Department to treat an ingrown nail in the inner corner of the left hallux. Examination of the entire nail unit showed the presence of xanthonychia in the outer corner besides thickening and increase in the transverse curvature of the nail plate. Dermoscopy and nuclear magnetic resonance of the free edge of the nail plate detected characteristic signs of onychomatricoma, a diagnosis that was later confirmed by anatomopathological exam.

Humans , Anticholesteremic Agents/therapeutic use , Cholesterol Ester Transfer Proteins/antagonists & inhibitors , Fibric Acids/therapeutic use , Lipoproteins, HDL/blood , Niacin/therapeutic use , Coronary Disease/blood , Coronary Disease/mortality , Coronary Disease/prevention & control , Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use , Myocardial Infarction/blood , Myocardial Infarction/mortality , Myocardial Infarction/prevention & control , Oxazolidinones/therapeutic use , Quinolines/therapeutic use , Randomized Controlled Trials as Topic , Stroke/blood , Stroke/mortality , Stroke/prevention & control , Sulfhydryl Compounds/therapeutic use
Article in English | IMSEAR | ID: sea-158404


Background & objectives: Recent data suggest that insulin resistance can predict cardiovascular disease independently of the other risk factors, such as hypertension, visceral obesity or dyslipidaemia. However, the majority of available methods to evaluate insulin resistance are complicated to operate, expensive, and time consuming. This study was undertaken to assess whether serum lipoprotein ratios could predict insulin resistance in non-diabetic acute coronary syndrome (ACS) patients. Methods: Ninety non-diabetic patients with impaired fasting glucose admitted with a diagnosis of ACS were included in the study. At the time of admission fasting glucose and insulin concentrations were measured. The homeostatic model assessment-insulin resistance (HOMA-IR) was used for insulin resistance. The fasting serum total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDL-C) levels were checked, and then TC/HDL-C and TG/HDL-C ratios were calculated. The areas under the curves (AUC) of the receiver operating characteristic (ROC) curves were used to compare the power of these serum lipoprotein ratios as markers. Results: Lipoprotein ratios were significantly higher in patients with HOMA-IR index > 2.5 as compared to patients with index <2.5 (P < 0.05). Both TG/HDL-C and TC/HDL-C ratios were significantly correlated with HOMA-IR (P<0.05). The area under the ROC curve of the TG/HDL-C and TC/HDL-C ratio for predicting insulin resistance was 0.80 (95% CI, 0.67 to 0.93), 0.78 (95% CI, 0.65 to 0.91), respectively. Interpretation & conclusions: The findings of this study demonstrate that serum lipoprotein ratios can provide a simple means of identifying insulin resistance and can be used as markers of insulin resistance and cardiovascular diseases risk in adult non-diabetic patients.

Acute Coronary Syndrome/complications , Biomarkers , Blood Glucose/analysis , Blood Glucose/blood , Female , Humans , India , Insulin Resistance/physiology , Lipoproteins/analysis , Lipoproteins/blood , Lipoproteins, HDL/analysis , Lipoproteins, HDL/blood , Male , Middle Aged , Triglycerides/analysis , Triglycerides/blood