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Article in English | WPRIM | ID: wpr-888616


BACKGROUND@#Remnant-like particle cholesterol (RLP-C) is highly atherogenic, which is associated with atherosclerosis. However, RLP-C has not been routinely measured in the clinical practice. We estimated RLP-C levels using conventional lipid profiles and examined the association between estimated RLP-C and related factors including nutrient intake.@*METHODS@#This study was performed in Uku town, Nagasaki prefecture, Japan in 2019. A total of 225 subjects were enrolled and directly measured RLP-C levels. Estimated RLP-C levels were defined as the following formula [total cholesterol - (LDL-cholesterol) - (HDL-cholesterol)]. Multivariate analyses were used to assess the relationship between estimated RLP-C and atherogenic factors. We calculated cut-off values on dichotomized RLP-C (< 7.5 mg/dL vs. ≥ 7.5 mg/dL) by receiver operating characteristic (ROC) curve.@*RESULTS@#The mean values of directly measured RLP-C levels and estimated RLP-C were 4.0 mg/dL and 16.4 mg/dL, respectively. In the multiple stepwise linear regression analysis, directly measured and estimated RLP-C levels were independently and commonly associated with apolipoprotein E, triglycerides, and vegetable fat intake (inversely). Using ROC curves, we found the cut-off value of estimated RLP-C was 22.0 mg/dL.@*CONCLUSION@#We demonstrated that the estimated RLP-C levels using conventional lipid profiles may substitute for directly measured RLP-C and these levels were independently and inversely associated with vegetable fat intake in the community-dwelling Japanese population.

Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Cholesterol/blood , Dietary Fats/blood , Japan , Lipids/blood , Lipoproteins/blood , Triglycerides/blood , Vegetables
Braz. j. med. biol. res ; 54(10): e11035, 2021. tab, graf
Article in English | LILACS | ID: biblio-1285659


In this eight-year retrospective study, we evaluated the associations between climatic variations and the biological rhythms in plasma lipids and lipoproteins in a large population of Campinas, São Paulo state, Brazil, as well as temporal changes of outcomes of cardiovascular hospitalizations. Climatic variables were obtained at the Center for Meteorological and Climatic Research Applied to Agriculture (University of Campinas - Unicamp, Brazil). The plasma lipid databases surveyed were from 27,543 individuals who had their lipid profiles assessed at the state university referral hospital in Campinas (Unicamp). The frequency of hospitalizations was obtained from the Brazilian Public Health database (DATASUS). Temporal statistical analyses were performed using the methods Cosinor or Friedman (ARIMA) and the temporal series were compared by cross-correlation functions. In normolipidemic cases (n=11,892), significantly different rhythmicity was observed in low-density lipoprotein (LDL)- and high-density lipoprotein (HDL)-cholesterol (C) both higher in winter and lower in summer. Dyslipidemia (n=15,651) increased the number and amplitude of lipid rhythms: LDL-C and HDL-C were higher in winter and lower in summer, and the opposite occurred with triglycerides. The number of hospitalizations showed maximum and minimum frequencies in winter and in summer, respectively. A coincident rhythmicity was observed of lower temperature and humidity rates with higher plasma LDL-C, and their temporal series were inversely cross-correlated. This study shows for the first time that variations of temperature, humidity, and daylight length were strongly associated with LDL-C and HDL-C seasonality, but moderately to lowly associated with rhythmicity of atherosclerotic outcomes. It also indicates unfavorable cardiovascular-related changes during wintertime.

Humans , Cardiovascular Diseases/epidemiology , Climate , Lipids/blood , Lipoproteins/blood , Periodicity , Seasons , Triglycerides/blood , Brazil/epidemiology , Retrospective Studies , Cholesterol, HDL/blood
Int. j. cardiovasc. sci. (Impr.) ; 32(6): 623-634, Nov.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1056381


Abstract Background: Vegetarian diets have been linked to reduced risk of chronic noncommunicable diseases, since they positively modulate biochemical parameters, particularly those related with glycemic control and lipemia, and considered as potential strategy for weight control. Objective: To compare the nutritional status, lifestyle and lipid profile of adult vegetarians with omnivores in a sample of individuals in the city of São Paulo. Methods: This was a cross-sectional study. Anthropometric, biochemical and lifestyle variables were compared between vegetarians and omnivores. A significance level of 5% was considered for all analyses. Results: Vegetarians were more likely to practice physical activity (64.3% vs 42.5%, p = 0.056) and consuming dietary supplements (48.1% vs 20.5%, p = 0.012). There was no statistically significant difference for the variables: age, sex, triglycerides, total cholesterol and low-density lipoprotein between the two groups. Vegetarians had significantly lower weight [60.8 kg (56.7 - 69.4) vs 71.1 kg (58.0 - 75.4), p = 0.038], BMI [22.4 kg/m2 (20.9 - 23.8) vs 24.6 kg/m2 (21.7 - 26.1), p = 0.001], and waist circumference [(81.8 ± 8.2 vs 87.8 ± 10.9 cm, p = 0.003)], and higher high-density lipoprotein (54.88 ± 14.44 vs 47.30 ± 12.27 mg /dL p = 0.008) than omnivores. Conclusion: Compared with omnivores, vegetarians had a better nutritional status, with lower BMI and waist circumference, significantly higher levels of plasma lipoprotein high-density, and healthier lifestyle.

Humans , Male , Adult , Diet, Vegetarian , Body Mass Index , Waist Circumference , Vegetarians , Body Weights and Measures , Exercise , Nutritional Status , Chronic Disease , Cross-Sectional Studies , Glycemic Control , Life Style , Lipoproteins/blood
Arq. bras. cardiol ; 113(2): 197-204, Aug. 2019. tab, graf
Article in English | LILACS | ID: biblio-1019399


Abstract Background: Elevated plasma levels of Lipoprotein(a) [Lp(a)] are recognized as a significant risk factor for atherosclerotic vascular disease. However, there are limited data regarding association between Lp(a) and recurrent heart failure (HF) in patients with chronic HF caused by coronary heart disease (CHD). Objective: Elevated levels of Lp(a) might have a prognostic impact on recurrent HF in patients with chronic HF caused by CHD. Methods: A total of 309 patients with chronic HF caused by CHD were consecutively enrolled in this study. The patients were divided into 2 groups according to whether Lp(a) levels were above or below the median level for the entire cohort (20.6 mg/dL): the high Lp(a) group (n = 155) and the low Lp(a) group (n = 154). A 2-sided p < 0.05 was statistically considered significant. Results: During the median follow-up period of 186 days, 31 cases out of a total of 309 patients (10.03%) could not be reached during follow-up. A Kaplan-Meier analysis demonstrated that patients with higher Lp(a) levels had a higher incidence of recurrent HF than those with lower Lp(a) levels (log-rank < 0.0001). A multivariate Cox regression analysis revealed that Lp(a) levels were independently correlated with the incidence of recurrent HF after adjustment of potential confounders (hazard ratio: 2.720, 95 % confidence interval: 1.730-4.277, p < 0.0001). Conclusions: In Chinese patients with chronic HF caused by CHD, elevated levels of Lp(a) are independently associated with recurrent HF.

Resumo Fundamento: Níveis plasmáticos elevados de lipoproteína (a) [Lp(a)] são reconhecidos como um fator de risco significativo para doença vascular aterosclerótica. No entanto, existem dados limitados sobre a associação entre a Lp(a) e insuficiência cardíaca (IC) recorrente em pacientes com IC crônica causada por doença arterial coronariana (DAC). Objetivo: Níveis elevados de Lp(a) podem ter um impacto prognóstico na IC recorrente em pacientes com IC crônica por DAC. Métodos: Um total de 309 pacientes com IC crônica causada por DAC foram consecutivamente incluídos neste estudo. Os pacientes foram divididos em 2 grupos de acordo com os níveis de Lp(a), acima ou abaixo do nível mediano de toda a coorte (20,6 mg/dL): o grupo Lp(a) alto (n = 155) e o grupo Lp ( a) baixo (n = 154). Um p < 0,05 bicaudal foi considerado estatisticamente significativo. Resultados: Durante a mediana do período de seguimento de 186 dias, 31 casos de um total de 309 pacientes (10,03%) não puderam ser contatados durante o acompanhamento. A análise de Kaplan-Meier demonstrou que pacientes com níveis mais elevados de Lp(a) apresentavam maior incidência de IC recorrente do que aqueles com níveis mais baixos de Lp(a) (log-rank < 0,0001). Uma análise de regressão multivariada de Cox revelou que os níveis de Lp(a) foram independentemente correlacionados com a incidência de IC recorrente após ajuste de potenciais fatores de confusão (hazard ratio 2,720, intervalo de confiança de 95%: 1,730-4,277, p < 0,0001). Conclusões: Em pacientes chineses com IC crônica causada por DAC, níveis elevados de Lp(a) estão associados de forma independente à IC recorrente.

Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Coronary Artery Disease/blood , Heart Failure/blood , Lipoproteins/blood , Recurrence , Reference Values , Time Factors , Coronary Artery Disease/complications , Echocardiography , Chronic Disease , Regression Analysis , Retrospective Studies , Risk Factors , Statistics, Nonparametric , Risk Assessment/methods , Kaplan-Meier Estimate , Heart Failure/etiology
Article in English | AIM | ID: biblio-1259669


Background: Hypertension as a global public health challenge is a major risk factor for cardiovascular (CVD) and coronary heart diseases (CHD) because of its chronic sequelae. It is accompanied by dyslipidemia and oxidative stress leading to increase in lipid peroxidation. This study aimed to measure the fasting serum lipid profile and malondialdehyde (MDA) and determine the atherogenic index as well as the cardiovascular risk ratio among hypertensive patients in Kano, Nigeria. Patients and Methods: Two hundred subjects (100 hypertensive patients vs. 100 normotensive controls) were recruited for the study. The fasting serum lipid profile and MDA were assayed using routine laboratory methods. Lipid ratios that predict and identify an individual's increased risk for cardiovascular diseases were then determined from the results of the profile. Results: The serum total cholesterol (7.0±0.5 vs 4.1±0.4 mmol/L), triglycerides (2.9±0.2 vs 2.0±0.3 mmol/Lg/dl), LDL cholesterol (3.8±0.4 vs 2.6±0.4 mmol/L), VLDL cholesterol (3.0±0.2 vs 2.1±0.2 mmol/L) and MDA (TBARS) (9×10-5±1.4×10-5 vs 3×106±0.9×10-6 mol/l) were significantly (p<0.05) increase in hypertensive patients compared to normotensive controls. HDL cholesterol was significantly higher (p<0.05) in normotensive controls compared to hypertensive patients (31.4±8 vs 23.9±6 mg/dl). A statistically significant (p<0.05) positive correlation was observed between LDL cholesterol and MDA only. Both the atherogenic index (AI) ratio and the CardioRisk ratio were significantly higher in Hypertensives than Normal controls (10.4 vs 4.1; 11.7 vs 5.1 respectively). Conclusion: This study demonstrated an increased occurrence of atherogenic lipid profile and oxidative stress among hypertensive patients. It further showed a strong correlation between dyslipidaemia and oxidative stress. Therapeutic lifestyle changes and use of statins should be considered an integral part of the treatment for hypertensive patients in Nigeria

Dyslipidemias , Hypertension , Lipid Peroxidation , Lipids , Lipoproteins/blood , Nigeria , Oxidative Stress
Rev. bras. ginecol. obstet ; 40(5): 281-286, May 2018. tab, graf
Article in English | LILACS | ID: biblio-958993


Abstract Several changes occur in lipid metabolism during gestation due to hormonal and metabolic changes, which are essential to satisfy the nutritional demands of the maternal-fetal unit development. The gestation shows two distinct periods that begin with fat accumulation, mainly in maternal adipose tissue, and the late phase, characterized by accelerated catabolism, with the increase of fatty acids in the circulation that causes hyperlipidemia, especially the one characterized as hypertriglyceridemia. Maternal hyperlipidemia may be associated with the development of maternal-fetal complications (preterm birth, preeclampsia, vascular complications) and the development of long-term cardiovascular disease. The cardiovascular risk may not only be related to lipoproteins cholesterol content, but also to the number and functionality of circulating lipoprotein particles. This review reports themajor changes that occur in lipoprotein metabolismduring pregnancy and that are associated with the development of dyslipidemias, lipoprotein atherogenic phenotype, and maternal-fetal unit complications.

Resumo Diversas mudanças ocorrem no metabolismo lipídico durante a gestação em função das alterações hormonais e metabólicas, que são essenciais para satisfazer a demanda nutricional ocasionada pelo desenvolvimento da unidade feto-placentária. O período da gestação apresenta dois momentos distintos que iniciam com acúmulo de gordura principalmente no tecido adiposo materno, e a fase tardia, caracterizada por catabolismo acelerado, com aumento de ácidos graxos na circulação causando hiperlipidemia, principalmente a aquela caracterizada como hipertrigliceridemia. A hiperlipidemia materna pode estar associada ao desenvolvimento de complicações materno-fetais (parto prematuro, pré-eclâmpsia, complicações vasculares) e de doenças cardiovasculares, a longo prazo. O risco pode estar relacionado não apenas ao teor de colesterol contido nas frações lipoprotéicas, mas também ao número e a funcionalidade das partículas lipoproteicas. Esta revisão aborda as principais mudanças que ocorrem no metabolismo lipoproteico durante a gravidez, e que estão associadas ao desenvolvimento de dislipidemias, fenótipo aterogênico e complicações maternofetais.

Humans , Female , Pregnancy , Pregnancy Complications/blood , Pregnancy Complications, Cardiovascular/blood , Fetal Diseases/blood , Lipoproteins/blood , Pregnancy Complications, Cardiovascular/epidemiology , Biomarkers/blood , Risk Assessment
Braz. j. med. biol. res ; 51(3): e6955, 2018. graf
Article in English | LILACS | ID: biblio-889047


The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing of samples immediately after collection.

Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Blood Specimen Collection/methods , Lipids/blood , Blood Chemical Analysis , Blood Preservation , Blood Specimen Collection/instrumentation , Blood Specimen Collection/standards , Cholesterol/blood , Laboratories/standards , Lipoproteins/blood , Prospective Studies , Temperature , Time Factors , Triglycerides/blood
Rev. bras. ginecol. obstet ; 38(12): 600-608, Dec. 2016. tab
Article in English | LILACS | ID: biblio-843887


ABSTRACT Purpose: Female sexual dysfunction is a complex and common condition that affects women, and the relationship between sexual function and dyslipidemia is poorly studied. This study aims to assess this relationship in the reproductive life women in the menacme who use combined oral contraceptives (COCs) . Methods: A total of 49 healthy women who were sexually active received COC pills that contained ethinylestradiol 30 mcg (EE30) plus levonorgestrel 150 mcg (LNG150). The women were divided into two groups according to their lipid profiles. Dyslipidemia was defined as a high-density lipoprotein (HDL) level < 50 mg/dL or a low-density lipoprotein (LDL) level > 130 mg/dL. Sexual function was assessed using the Female Sexual Function Index (FSFI) Questionnaire. Lipid and lipoprotein parameters were obtained at baseline and after the sixth cycle. Results: After six cycles of the COCs, the total cholesterol and LDL cholesterol levels in the women with a LDL level > 130 mg/dL decreased by 14.7% and 22.1% respectively. In the women with a HDL level < 50 mg/dL at baseline, the HDL level increased by 15.5% at the end of the study. The arousal and orgasm domains and the FSFI total scores significantly increased in women with and without dyslipidemia. The desire and satisfaction domains increased only in the group without dyslipidemia at the end of the treatment period. Conclusions: The EE30/LNG150 formulation increased the sexual function and it was only positively correlated with the HDL cholesterol level. These data indicated a low correlation between sexual function and the changes in the lipid and lipoprotein metabolism.

RESUMO Objetivo: Disfunção sexual feminina é uma condição complexa acomete as mulheres, e a relação entre a função sexual e a dislipidemia é muito pouco estudada. Este estudo objetivou avaliar esta relação em mulheres na menacme que fazem uso de contraceptivos orais combinados (COCs). Métodos: Um total de 49 mulheres saudáveis com vida sexual ativa receberam pílulas anticoncepcionais contendo etinilestradiol 30 mcg (EE30) associado a levonorgestrel 150 mcg (LNG150). As mulheres foram divididas em dois grupos, de acordo com o perfil lipídico. Dislipidemia foi definida como nível de lipoproteína de alta densidade (HDL) < 50 mg/dL, ou nível de lipoproteína de baixa densidade (LDL) > 130 mg/dL. A função sexual feminina foi avaliada utilizando o questionário de Índice de Função Sexual Feminina (IFSF). O IFSF e os parâmetros lipídicos e lipoproteicos foram obtidos no início e após o sexto ciclo do estudo. Resultados: Após seis ciclos de uso dos COCs, as mulheres com LDL > 130 mg/dL, tiveram redução dos níveis de colesterol total e colesterol LDL de 14,7% e 22,1% respectivamente. Nas mulheres com níveis HDL < 50 mg/dL no momento basal, o nível de HDL aumentou 15,5% ao final do estudo. Os domínios de excitação, orgasmo e os escores totais do IFSF aumentaram significativamente nas mulheres com e sem dislipidemia. Os domínios de desejo e satisfação aumentaram no final do período de tratamento exclusivamente no grupo sem dislipidemia. Conclusões: A formulação EE30/LNG150 aumentou a função sexual das mulheres, sendo positivamente correlata somente com os níveis de colesterol HDL. Estes achados demonstram baixa correlação entre a função sexual e as alterações no metabolismo lipídico e lipoproteico.

Humans , Female , Contraceptives, Oral, Combined/therapeutic use , Dyslipidemias/drug therapy , Sexual Dysfunction, Physiological/drug therapy , Dyslipidemias/blood , Dyslipidemias/complications , Lipids/blood , Lipoproteins/blood , Orgasm/drug effects , Sexual Behavior , Sexual Dysfunction, Physiological/blood , Sexual Dysfunction, Physiological/complications
Rev. Soc. Cardiol. Estado de Säo Paulo ; 26(3): 195-204, jul.-set. 2016. tab, ilus
Article in Portuguese | LILACS | ID: biblio-832613


O Diabetes mellitus é uma doença com elevada prevalência global, associada à mortalidade cardiovascular e complicações microvasculares, que conferem o caráter crônico a essa patologia. No diabetes tipo II, o processo aterosclerótico tem início antes mesmo do diagnóstico, daí a importância do reconhecimento dos fatores de risco implicados na fisiopatologia da doença vascular nessa população. A dislipidemia no diabético é caracterizada por lipoproteínas ricas em triglicérides, LDL pequenas, densas e muito aterogênicas e HDL-c baixo. As estatinas são os medicamentos de escolha para tratar a dislipidemia e reduzir de forma significativa o risco cardiovascular nesses pacientes. Apesar do controle glicêmico intensivo não reduzir eventos cardiovasculares nos estudos randomizados, alguns hipoglicemiantes apresentam efeito favorável sobre o perfil lipídico, com redução de futuros eventos

Diabetes mellitus is a disease with high global prevalence, associated with cardiovascular mortality and microvascular complications, which give this disease its chronic nature. In type II diabetes, the atherosclerotic process begins even before diagnosis, hence the importance of recognizing the risk factors involved in pathophysiology of vascular disease in this population. Dyslipidemia in the diabetic patient is characterized by triglyceride-rich lipoproteins; small-dense and very atherogenic LDLs and low HDL-c. Statins are the drugs of choice for treating dyslipidemia and significantly reducing the cardiovascular risk in these patients. Although intensive glycemic control did not reduce cardiovascular events in randomized trials, some hypoglycemic drugs have demonstrated a favorable effect on the lipid profile, and may reduce future events

Humans , Insulin Resistance , Diabetes Mellitus/diagnosis , Dyslipidemias/complications , Dyslipidemias/diagnosis , Hypertension/complications , Hypertension/physiopathology , Cardiovascular Diseases/physiopathology , Chronic Disease , Risk Factors , Lipoproteins/analysis , Lipoproteins/blood , Cholesterol, HDL/analysis , Cholesterol, HDL/blood , Cholesterol, LDL/analysis , Cholesterol, LDL/blood , Liver/physiopathology
IJFS-International Journal of Fertility and Sterility. 2016; 10 (1): 29-35
in English | IMEMR | ID: emr-178863


Background: Dyslipidemia and insulin resistance [IR], occurring in most infertile women with polycystic ovarian syndrome [PCOS], increase the risk of cardiovascular disease [CVD] and type 2 diabetes. This study aimed to assess the relationships between lipopro-tein ratios and IR in PCOS women

Materials and Methods: Thirty six infertile women with PCOS selected based on Andro-gen Excess Society [AES] criteria and 29 healthy women matched for age were recruited to this case-control study. After physical measurements, fasting serum glucose [Glu], insulin and lipid profile levels [triglycerides [TGs], total cholesterol [TC], low-density lipoprotein-cholesterol [LDL-C] and high-density lipoprotein-cholesterol [HDL-C]] were measured, while lipoprotein ratios [TC/HDL-C, LDL-C/HDL-C, TG/HDL-C] were calculated. IR was also calculated using homeostasis model assessment [HOMA]-IR. The optimal cutoffs of lipoprotein ratios in relation to HOMA-IR were calculated based on the Receiver Operating Characteristics [ROC] curve analysis using the area under curve [AUC]

Results: Waist circumference [WC], insulin levels, HOMA-IR, TG levels, and all lipoprotein ratios were significantly higher, while HDL-C was lower in PCOS group as compared to healthy controls. All lipoprotein ratios, TG levels, and WC are significantly correlated with insulin levels and HOMA-IR. Among lipoprotein ratios, the highest AUC of the ROC belonged to TG/HDL-C ratio with sensitivity of 63.6% and specificity of 84.4% [TG/HDL-O3.19] as a marker of IR in infertile PCOS women

Conclusion: Lipoprotein ratios, particularly TG/HDL-C, are directly correlated with insulin levels and can be used as a marker of IR [HOMA-IR] in infertile PCOS patients

Humans , Women , Adult , Lipoproteins/blood , Insulin Resistance , Infertility, Female , Case-Control Studies
Arq. bras. cardiol ; 105(1): 45-52, July 2015. tab
Article in English | LILACS | ID: lil-755005



Evidences suggest that paraoxonase 1 (PON1) confers important antioxidant and anti-inflammatory properties when associated with high-density lipoprotein (HDL).


To investigate the relationships between p.Q192R SNP of PON1, biochemical parameters and carotid atherosclerosis in an asymptomatic, normolipidemic Brazilian population sample.


We studied 584 volunteers (females n = 326, males n = 258; 19-75 years of age). Total genomic DNA was extracted and SNP was detected in the TaqMan® SNP OpenArray® genotyping platform (Applied Biosystems, Foster City, CA). Plasma lipoproteins and apolipoproteins were determined and PON1 activity was measured using paraoxon as a substrate. High-resolution β-mode ultrasonography was used to measure cIMT and the presence of carotid atherosclerotic plaques in a subgroup of individuals (n = 317).


The presence of p.192Q was associated with a significant increase in PON1 activity (RR = 12.30 (11.38); RQ = 46.96 (22.35); QQ = 85.35 (24.83) μmol/min; p < 0.0001), HDL-C (RR= 45 (37); RQ = 62 (39); QQ = 69 (29) mg/dL; p < 0.001) and apo A-I (RR = 140.76 ± 36.39; RQ = 147.62 ± 36.92; QQ = 147.49 ± 36.65 mg/dL; p = 0.019). Stepwise regression analysis revealed that heterozygous and p.192Q carriers influenced by 58% PON1 activity towards paraoxon. The univariate linear regression analysis demonstrated that p.Q192R SNP was not associated with mean cIMT; as a result, in the multiple regression analysis, no variables were selected with 5% significance. In logistic regression analysis, the studied parameters were not associated with the presence of carotid plaques.


In low-risk individuals, the presence of the p.192Q variant of PON1 is associated with a beneficial ...


Evidências sugerem que a paroxonase 1 (PON1) confere importantes propriedades antioxidantes e antiinflamatórias quando associada à lipoproteína de alta densidade (HDL).


Investigar as relações entre o SNP p.Q192R da PON1, parâmetros bioquímicos e aterosclerose carotídea em uma amostra populacional brasileira assintomática e normolipidêmica.


Foram estudados 584 voluntários (mulheres, n = 326; homens, n = 258; idade entre 19-75 anos). Foi extraído DNA genômico total e o SNP foi detectado na plataforma de genotipagem TaqMan® SNP OpenArray® (Applied Biosystems, Foster City, CA). Foram dosadas lipoproteínas e apolipoproteínas plasmáticas, e a atividade da PON1 foi medida utilizando-se paraoxon como substrato. Foi utilizada ultrassonografia bidimensional de alta resolução para determinar a espessura íntimo‑medial das artérias carótidas (EIMc) e a presença de placas ateroscleróticas carotídeas em um subgrupo de indivíduos (n = 317).


A presença de p.192Q esteve associada a um aumento significativo da atividade da PON1 (RR = 12,30 (11,38); RQ = 46,96 (22,35); QQ = 85,35 (24.83) μmol/min; p < 0,0001), HDL-C (RR = 45 (37); RQ = 62 (39); QQ= 69 (29) mg/dL; p < 0,001) e apo A-1 (RR = 140,76 ± 36,39; RQ = 147,62 ± 36,92; QQ = 147,49 ± 36,65 mg/dL; p = 0,019). A análise de regressão stepwise mostrou que heterozigotos e portadores de p.192Q influenciaram 58% da atividade da PON1 em relação ao paraoxon. A análise de regressão linear univariada demonstrou que não houve associação entre o SNP p.Q192R e a EIMc média; como resultado, na análise de regressão múltipla nenhuma variável foi selecionada com 5% de significância. Os parâmetros estudados não se associaram à presença de placas carotídeas na análise de regressão logístic...

Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Aryldialkylphosphatase/genetics , Carotid Artery Diseases/genetics , Lipoproteins/genetics , Polymorphism, Single Nucleotide , Aryldialkylphosphatase/blood , Brazil , Carotid Intima-Media Thickness , Carotid Artery Diseases/ethnology , Carotid Artery Diseases , Genetic Association Studies , Lipoproteins/blood , Real-Time Polymerase Chain Reaction , Reference Values , Regression Analysis , Risk Factors
Arq. bras. cardiol ; 104(4): 266-273, 04/2015. tab
Article in English | LILACS | ID: lil-745743


Introduction: Obesity-related comorbidities are present in young obese children, providing a platform for early adult cardiovascular disorders. Objectives: To compare and correlate markers of adiposity to metabolic disturbances, vascular and cardiac morphology in a European pediatric obese cohort. Methods: We carried out an observational and transversal analysis in a cohort consisting of 121 obese children of both sexes, between the ages of 6 and 17 years. The control group consisted of 40 children with normal body mass index within the same age range. Markers of adiposity, plasma lipids and lipoproteins, homeostasis model assessment-insulin resistance, common carotid artery intima-media thickness and left ventricular diameters were analyzed. Results: There were statistically significant differences between the control and obese groups for the variables analyzed, all higher in the obese group, except for age, high-density lipoprotein cholesterol and adiponectin, higher in the control group. In the obese group, body mass index was directly correlated to left ventricular mass (r=0.542; p=0.001), the homeostasis model assessment-insulin resistance (r=0.378; p=<0.001) and mean common carotid artery intima-media thickness (r=0.378; p=<0.001). In that same group, insulin resistance was present in 38.1%, 12.5% had a combined dyslipidemic pattern, and eccentric hypertrophy was the most common left ventricular geometric pattern. Conclusions: These results suggest that these markers may be used in clinical practice to stratify cardiovascular risk, as well as to assess the impact of weight control programs. .

Fundamento: As comorbidades relacionadas com a obesidade encontram-se patentes em crianças jovens obesas e são, potencialmente, um ponto de partida para as doenças cardiovasculares em adultos jovens. Objetivos: Comparar e correlacionar marcadores de adiposidade com distúrbios metabólicos e alterações cardiovasculares numa coorte de crianças obesas europeias. Métodos: Estudo observacional e transversal de uma coorte composta por 121 crianças obesas de ambos os gêneros, entre 6 e 17 anos de idade. O grupo controle incluiu 40 crianças com índice de massa corporal normal dentro da mesma faixa etária. Analisamos marcadores de adiposidade, lípides e lipoproteínas, o índice de insulino-resistência, a espessura da camada íntima-média da artéria carótida comum e os diâmetros do ventrículo esquerdo. Resultados: Observamos diferenças significativas entre os grupos controle e obeso para todos os parâmetros em análise, com todos os valores mais elevados no grupo obeso, exceto a idade, a lipoproteína de alta densidade e a adiponectina, superiores no grupo controle. No grupo obeso, o índice de massa corporal correlacionou‑se diretamente com a massa ventricular esquerda (r=0,542; p=0,001), com o índice de insulino-resistência (r = 0,378; p = < 0,001) e com a espessura da camada íntima-média da artéria carótida comum (r = 0,378; p = <0,001). Ainda no grupo obeso, 38,1% tinham insulino-resistência, 12,5% apresentavam um padrão de dislipidemia combinada, e hipertrofia excêntrica foi a forma geométrica ventricular mais observada. Conclusões: Os resultados obtidos sugerem que os marcadores analizados podem ser utilizados para aferir risco cardiovascular, assim como para avaliar o impacto analítico e morfológico dos programas de redução de peso. .

Adolescent , Child , Female , Humans , Male , Adiposity , Dyslipidemias , Insulin Resistance , Pediatric Obesity , Age Factors , Acanthosis Nigricans/complications , Acanthosis Nigricans/diagnosis , Adiponectin/blood , Body Mass Index , Biomarkers/blood , Carotid Intima-Media Thickness , Case-Control Studies , Cross-Sectional Studies , Cardiovascular Diseases/etiology , Cardiovascular Diseases/prevention & control , Dyslipidemias/blood , Dyslipidemias/complications , Hypertrophy, Left Ventricular/pathology , Leptin/blood , Lipoproteins/blood , Pediatric Obesity/blood , Pediatric Obesity/complications , Risk Factors , Sex Factors , Waist Circumference
Int. j. cardiovasc. sci. (Impr.) ; 28(1): 9-15, jan.-fev. 2015. tab, graf
Article in English, Portuguese | LILACS | ID: lil-762184


Fundamentos: Estudos apontam que durante a lipemia pós-prandial (LPP) ocorre formação de radicais livres estimulando o endotélio a secretar citocinas que vão mediar a resposta inflamatória. O excesso de tecido adiposo, especialmente na região abdominal, correlaciona-se positivamente com os valores da proteína C-reativa (PCR). No entanto, pouco se sabe sobre a variação da PCR na fase inicial da LPP especialmente em indivíduos obesos. Objetivo: Verificar se existe variação na concentração plasmática da PCR na fase inicial da LPP em indivíduos com obesidade central. Métodos: Avaliados 40 indivíduos de ambos os sexos, sedentários, sem alteração do perfil lipídico de jejum e comcircunferência de cintura acima do ponto de corte considerado normal. Em jejum de 12 horas e três horas após a ingestão de 50g de gordura foi dosada a PCR. Resultados: Os valores da mediana da PCR de jejum e três horas após a ingestão de lipídeos foram, respectivamente: 0,6 mg/L (0,2-1,8 mg/L) e 0,4 mg/L (0,2-1,8 mg/L) (p=1,000). Conclusão: Neste estudo a fase inicial da LPP não induziu variação da concentração da PCR em indivíduos com obesidade central.

Background: Studies indicate that during postprandial lipemia (PPL), free radical formation occurs, stimulating the endothelium to secrete cytokines that mediate inflammatory responses. Excess adipose tissue, especially in the abdominal region, is positively correlated with C-reactive protein (CRP) values. However, little is known about CRP variations during the initial phase of PPL, especially among obese individuals. Objective: To determine if there are variations in CRP plasma concentrations among individuals with central obesity during the initial phase of PPL. Methods: This study assessed forty sedentary men and women with no alterations to fasting lipid profiles and waist circumferences above the normal cut off point, measuring their CRP levels after fasting for twelve hours and three hours after ingesting 50g of fat. Results: The mean CRP values after fasting and three hours after lipids intake were 0.6 mg/L (0.2 to 1.8 mg/L) and 0.4 mg/L(0.2 to 1.8mg/L) (p=1.000) respectively. Conclusion: In this study, the initial phase of PPL did not present any variations in CRP concentrations among subjects with central obesity.

Humans , Male , Female , Young Adult , C-Reactive Protein , Lipids/blood , Obesity, Abdominal , Postprandial Period , Body Mass Index , Abdominal Fat/metabolism , Inflammation , Lipoproteins/blood , Prospective Studies , Sex Factors , Triglycerides/blood , Waist Circumference
Article in English | IMSEAR | ID: sea-158404


Background & objectives: Recent data suggest that insulin resistance can predict cardiovascular disease independently of the other risk factors, such as hypertension, visceral obesity or dyslipidaemia. However, the majority of available methods to evaluate insulin resistance are complicated to operate, expensive, and time consuming. This study was undertaken to assess whether serum lipoprotein ratios could predict insulin resistance in non-diabetic acute coronary syndrome (ACS) patients. Methods: Ninety non-diabetic patients with impaired fasting glucose admitted with a diagnosis of ACS were included in the study. At the time of admission fasting glucose and insulin concentrations were measured. The homeostatic model assessment-insulin resistance (HOMA-IR) was used for insulin resistance. The fasting serum total cholesterol (TC), triglycerides (TG) and high density lipoprotein cholesterol (HDL-C) levels were checked, and then TC/HDL-C and TG/HDL-C ratios were calculated. The areas under the curves (AUC) of the receiver operating characteristic (ROC) curves were used to compare the power of these serum lipoprotein ratios as markers. Results: Lipoprotein ratios were significantly higher in patients with HOMA-IR index > 2.5 as compared to patients with index <2.5 (P < 0.05). Both TG/HDL-C and TC/HDL-C ratios were significantly correlated with HOMA-IR (P<0.05). The area under the ROC curve of the TG/HDL-C and TC/HDL-C ratio for predicting insulin resistance was 0.80 (95% CI, 0.67 to 0.93), 0.78 (95% CI, 0.65 to 0.91), respectively. Interpretation & conclusions: The findings of this study demonstrate that serum lipoprotein ratios can provide a simple means of identifying insulin resistance and can be used as markers of insulin resistance and cardiovascular diseases risk in adult non-diabetic patients.

Acute Coronary Syndrome/complications , Biomarkers , Blood Glucose/analysis , Blood Glucose/blood , Female , Humans , India , Insulin Resistance/physiology , Lipoproteins/analysis , Lipoproteins/blood , Lipoproteins, HDL/analysis , Lipoproteins, HDL/blood , Male , Middle Aged , Triglycerides/analysis , Triglycerides/blood
European J Med Plants ; 2014 Dec; 4(12): 1489-1500
Article in English | IMSEAR | ID: sea-164213


Aims: Today there are concerns about possible adverse effects of dietary sugars. This study was set up to compare the metabolic dysfunction induced by dietary fructose in male rats with that of the female, investigate the modulatory effect of Loranthus micranthus on this dysfunction and compare this with that of nifedipine. Study Design: Fifty six rats assigned to four groups of 7 male and 7 females (hosted in different cages) per group were used in the study. The water of group B, C and D rats were supplemented with 10% fructose for the first two 2 weeks and was later increased to 20%, 30% and 40% fructose after every 2 other weeks respectively. Nifedipine (10mg/Kg) was administered to group C while L. micranthus (600mg/Kg) was orally administered to group D. All administrations were carried out daily as a single dose after which the rats were sacrificed and the serum analyzed for the lipid components. The serum glucose level was also measured after every 2 weeks interval. Results: Fructose administration increased serum total cholesterol, triglyceride, LDL-C, VLDL-C, atherogenic and coronary risk indexes but decreased serum HDL-C significantly. The increase was greater in the male rats. Serum glucose was not altered during the first 6 weeks of study but was observed to be significantly increased above the initial value after 8 weeks of study. Both L. micranthus and nifedipine prevented this metabolic dysfunction but the effect was more pronounced with L. micranthus extract. Conclusion: The study concludes that male subjects are more prone to metabolic dysfunction of fructose than the female group and that L. micranthus is efficacious in preventing this defect in both male and female subject.

Animals , Cardiovascular Diseases/drug therapy , Cardiovascular Diseases/metabolism , Cholesterol/blood , Female , Fructose/metabolism , Lipoproteins/blood , Loranthaceae/therapeutic use , Male , Plant Extracts/therapeutic use , Plant Leaves/therapeutic use , Rats , Risk Factors
Einstein (Säo Paulo) ; 12(4): 480-484, Oct-Dec/2014. tab
Article in English | LILACS | ID: lil-732462


Objective To evaluate the relation between oxidative stress and lipid profile in patients with different types of cancer. Methods This was an observational cross-sectional. A total of 58 subjects were evaluated, 33 males, divided into two groups of 29 patients each: Group 1, patients with cancer of the digestive tract and accessory organs; Group 2 patients with other types of cancers, all admitted to a public hospital. The plasma levels (lipoproteins and total cholesterol, HDL, and triglycerides, for example) were analyzed by enzymatic kits, and oxidative stress based on thiobarbituric acid-reactive substances, by assessing the formation of malondialdehyde. Results In general the levels of malondialdehyde of patients were high (5.00μM) as compared to 3.31μM for healthy individuals. The median values of lipids exhibited normal triacylglycerol (138.78±89.88mg/dL), desirable total cholesterol values (163.04±172.38mg/dL), borderline high LDL (151.30±178.25mg/dL) and low HDL (31.70±22.74mg/dL). Median HDL levels in Group 1 were lower (31.32mg/dL) than the cancer patients in Group 2 (43.67mg/dL) (p=0.038). Group 1 also showed higher levels of oxidative stress (p=0.027). Conclusion The lipid profile of patients with cancer was not favorable, which seems to have contributed to higher lipid peroxidation rate, generating a significant oxidative stress. .

Objetivo Avaliar a relação entre estresse oxidativo e perfil lipídico em pacientes com diferentes tipos de câncer. Métodos Estudo do tipo observacional transversal. Foram avaliados 58 indivíduos, sendo 33 do sexo masculino, divididos em dois grupos de 29 pacientes cada: o Grupo 1 foi formado por pacientes com câncer do trato digestivo e órgãos anexos; o Grupo 2 foi formado por pacientes com outros tipos de câncer, todos internados em um hospital público. Foram analisadas concentrações plasmáticas (colesterol total, HDL e triacilglicerol, entre outras), por meio de kits enzimáticos, e o estresse oxidativo, com base em substâncias reativas ao ácido tiobarbitúrico, por meio da formação de malondialdeído. Resultados Na mediana, os pacientes apresentaram valores altos de malondialdeído (5,00μM) quando comparados ao valor de 3,31μM para indivíduos saudáveis. A mediana do perfil lipídico exibiu valores de triacilgliceróis normais (138,78±89,88mg/dL), colesterol total com valores desejáveis (163,04±172,38mg/dL), LDL com limiar elevado (151,30±178,25mg/dL) e HDL baixo (31,70±22,74mg/dL). Os níveis medianos de HDL do Grupo 1 foram mais baixos (31,32mg/dL) do que dos pacientes oncológicos do Grupo 2 (43,67mg/dL) (p=0,038). O Grupo 1 apresentou níveis mais altos de estresse oxidativo (p=0,027). Conclusão O perfil lipídico de pacientes com câncer apresentou-se desfavorável, o que parece ter contribuído para uma maior taxa de peroxidação lipídica, gerando um significativo estresse oxidativo. .

Female , Humans , Male , Lipoproteins/blood , Neoplasms/metabolism , Oxidative Stress/physiology , Cross-Sectional Studies , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cholesterol/blood , Gastrointestinal Neoplasms/metabolism , Lipid Peroxidation , Malondialdehyde/analysis , Malondialdehyde/blood , Reference Values , Risk Factors , Statistics, Nonparametric , Triglycerides/blood
J. vasc. bras ; 13(4): 312-317, Oct-Dec/2014. tab
Article in English | LILACS | ID: lil-736019


Strength training is often identified as a contributing factor in prevention of diseases and as a non-pharmacological treatment for metabolic disorders and for control of body mass. Its protective effects and utility for management of disease are amplified in people at risk of diabetes mellitus and dyslipidemias, and cardiovascular diseases (CVD). Recently the benefits of strength training have been used to reduce the risk of these diseases emerging in postmenopausal women, who are at greater risk of CVD than men of the same age. Notwithstanding, little is known about the effects of strength training on metabolism of blood lipoproteins. The objective of this review was to compare the results of articles that have investigated the effects on lipoprotein concentrations of strength training in postmenopausal women. Current articles dealing with the subject, with publication dates from 1979 to 2012 and large numbers of citations by well-known researchers were identified on the Pubmed, Scopus and EBSCO databases. It was concluded that strength training possibly has an action that affects lipoprotein metabolism and concentrations in postmenopausal women.

O treinamento de força tem sido frequentemente relacionado como contribuinte para a prevenção do aparecimento de doenças e como tratamento não farmacológico para distúrbios metabólicos e controle de massa corporal. Os efeitos protetores e de manejo de doenças se ampliam para sujeitos com riscos para diabetes mellitus e dislipidemias, e doenças cardiovasculares (DCV). Recentemente, os benefícios do treinamento de força têm sido usados para reduzir o risco do aparecimento dessas doenças em mulheres pós-menopausa, as quais apresentam um risco maior para desenvolver DCV quando comparadas aos homens de mesma idade. Entretanto, pouco se sabe sobre a efetividade do treinamento de força sobre metabolismo das lipoproteínas sanguíneas. O objetivo desta revisão foi comparar os resultados de artigos que abordaram os efeitos do treinamento de força em mulheres pós-menopausa e quais os resultados sobre as concentrações de lipoproteínas. Foram pesquisadas referências atuais sobre o tema a ser abordado, sendo que os artigos foram selecionados nas bases de dados Pubmed, Scopus e EBSCO, artigos atuais datados do período de 1979 a 2012, com grande número de citações de pesquisadores renomados no assunto. Em conclusão, o treinamento de força possivelmente tenha uma ação efetiva no metabolismo e na concentração de lipoproteínas em mulheres pós-menopausa.

Humans , Female , Postmenopause/metabolism , Resistance Training , Lipoproteins/blood , Body Weight , Metabolic Syndrome , Fatty Acids , Heart Disease Risk Factors , Lipoproteins/metabolism
Braz. j. phys. ther. (Impr.) ; 18(5): 462-469, 12/09/2014. tab
Article in English | LILACS | ID: lil-727049


Background: It is unclear whether early physical activity has a greater influence on intima-media thickness and metabolic variables than current physical activity. Objective: To analyze the relationship between current and early physical activity, metabolic variables, and intima-media thickness measures in adults. Method: The sample was composed of 55 healthy subjects of both sexes (33 men and 22 women). Total body fat and trunk fat were estimated by dual-energy X-ray absorptiometry. Carotid and femoral intima-media thickness were measured using a Doppler ultrasound device. A 12-hour fasting blood sample collection was taken (fasting glucose and lipid profile). Early physical activity was assessed through face-to-face interview, and the current physical activity was assessed by pedometer (Digi-Walker Yamax, SW200), which was used for a period of seven days. Results: Current physical activity was negatively related to total cholesterol (rho=-0.31), while early physical activity was negatively related to triglycerides (rho=-0.42), total cholesterol (rho=-0.28), very low density lipoprotein (rho=-0.44), and carotid intima-media thickness (rho=-0.50). In the multivariate model, subjects engaged in sports activities during early life had lower values of very low density lipoprotein (b=-8.74 [b=-16.1; -1.47]) and carotid intima-media thickness (b=-0.17 [95%CI: -0.28; -0.05]). Conclusion: Early 95%CI physical activity has a significant influence on carotid intima-media thickness, regardless of the current physical activity. .

Humans , Male , Female , Adult , Exercise , Carotid Intima-Media Thickness , Triglycerides/blood , Blood Glucose/analysis , Cholesterol/blood , Age Factors , Lipoproteins/blood