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Int. j. morphol ; 41(6): 1837-1845, dic. 2023. ilus, tab
Article in English | LILACS | ID: biblio-1528786


SUMMARY: The potential anti-inflammatory and antifibrotic activity of polyphenolic extracts of blueberry and grape was evaluated in a mouse model of lung damage induced by subcutaneous administration of bleomycin. The results of testing the polyphenolic extracts on two different systemic administration variants of bleomycin (intraperitoneal and subcutaneous) were compared. It was found that regardless of the method of bleomycin administration, indirect cross-acute and subacute damage to the pulmonary system was observed. Both patterns exhibited the same prevalence and severity. The administration of polyphenolic extracts of blueberry and grape to mice resulted in a significant decrease in theseverity of acute and subacute patterns of lung damage, suggesting their protective properties for the microcirculatory bed and a pronounced anti-inflammatory effect.

La potencial actividad antiinflamatoria y antifibrótica de los extractos polifenólicos de arándano y uva se evaluó en un modelo de daño pulmonar en ratón inducido por la administración subcutánea de bleomicina. Se compararon los resultados de las pruebas de los extractos polifenólicos en dos variantes diferentes de administración sistémica de bleomicina (intraperitoneal y subcutánea). Se encontró que, independientemente del método de administración de bleomicina, se observaba daño indirecto cruzado, agudo y subagudo al sistema pulmonar. Ambos patrones exhibieron la misma prevalencia y gravedad. La administración de extractos polifenólicos de arándano y uva a ratones dio como resultado una disminución significativa en la gravedad de los patrones agudos y subagudos de daño pulmonar, lo que sugiere sus propiedades protectoras del lecho micro- circulatorio y un efecto antiinflamatorio pronunciado.

Animals , Mice , Bleomycin/toxicity , Plant Extracts/administration & dosage , Lung Injury/chemically induced , Lung Injury/drug therapy , Polyphenols/administration & dosage , Blueberry Plants/chemistry , Vitis/chemistry , Disease Models, Animal , Lung Injury/pathology , Lung/drug effects , Anti-Inflammatory Agents/administration & dosage
Chinese Journal of Industrial Hygiene and Occupational Diseases ; (12): 375-378, 2023.
Article in Chinese | WPRIM | ID: wpr-986017


Ingestion of corrosive substances can severely burn the upper digestive tract leading to bleeding or perforation, and may even be life-threatening. Less commonly, damage to the trachea and bronchi is involved. In this paper, a case of corrosive digestive tract injury and lung injury after oral administration of pipeline dredging agent (the main components are hydroxide, sodium carbonate, sodium hypochlorite, etc.) was analyzed. After active rescue treatment, the patient died of massive hemoptysis. It is suggested that serious complications may occur after ingestion of corrosive substances. Timely diagnosis and reasonable medical management are needed to improve the level of recognition and treatment of such diseases.

Humans , Caustics , Lung Injury/chemically induced , Gastrointestinal Tract , Burns, Chemical/therapy , Eating
Braz. arch. biol. technol ; 62: e19180096, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055376


Abstract Clinical research has shed the light on the relation between coagulation and inflammation. Coagulation cascade is activated in lung injury resulting in thrombotic and fibrotic lesions. Such a cascade is initiated by inflammation, then the two systems intense each other. New therapies that modulate coagulation and inflammation will be more successful than therapies targeting only one of them. Mesenchymal stem cells showed anti-inflammatory functions in animal models. The role of mesenchymal stem cells in methotrexate induced lung injury model was evaluated, but no studies scoped on the role of stem cells in coagulation associated with inflammation in such models. This study focuses on the therapeutic role of mesenchymal stem cells against the development of clotting in methotrexate induced lung injury rat model. Results showed that mesenchymal stem cells treatment for 4 weeks caused a decrease in lung activated coagulation factors; protease activated receptor-1, fibrinogen, plasminogen activator inhibitor-1 and platelet count with a decrease in inflammatory factors; tumor necrosis factor-α, interferon- γ, interleukin-8, monocyte chemoattractant protein-1 and total leukocyte count. Thus, mesenchymal stem cells have anti-inflammatory potency against clotting risk in methotrexate induced lung injury model. This opens the outlook for stem cells as a new therapy that moderates coagulation associated with inflammation.

Animals , Rats , Blood Coagulation , Methotrexate/administration & dosage , Lung Injury/chemically induced , Mesenchymal Stem Cells/drug effects , Inflammation/drug therapy , Models, Animal
Int. j. morphol ; 37(1): 87-92, 2019. tab, graf
Article in English | LILACS | ID: biblio-990010


SUMMARY: This study aimed to investigate the toxic effects of cigarette smoke exposure on lung and the protective role of Omega 3 and Vitamin D against these toxic effects biochemically and histologically. 28 pregnant Wistar Albino rats were divided into four groups. The first group was control group; the second group was exposed to smoke of 10 cigarette by puff device 2 hours/day after pregnancy; the third group was exposed to cigarette smoke together with Omega 3 (0.5 mg/kg/day) and the fourth group was exposed to cigarette smoke together with vitamin D (42 microgram/kg/day). Finally, lung tissue sections of the newborn rats were stained with Hemotoxilen eosine and Masson tricromite. Malondialdehyde (MDA) and Fluorescent Oxidation Products (FOU) levels were measured. Fetal weights and the number of fetuses were significantly lower in the group received only cigarette smoke (both p<0.001). Histopathologically, pulmonary volume, number of developed alveols and parenchyma elasticity decreased significantly, meanwhile interstitial tissue increased, elastin and collagen did not develop adequately. Histopathologic changes significantly decreased in the group given Omega 3 and Vitamin D. Statistically, MDA and FOU levels were found to be higher in the group exposed to cigarette smoke compared to the control group, and MDA and FOU levels were lower in the group given Omega 3 along with cigarette smoke (p<0.001). Cigarette smoke caused histologically significant damage to fetal lung tissue, oxidative stress and increased MDA and FOU levels. This damage was significantly reduced with Omega 3 and Vitamine D supplementation. Omega 3 is an important antioxidant; vitamin D has no significant antioxidant effect.

RESUMEN: Este estudio tuvo como objetivo investigar los efectos tóxicos de la exposición al humo de cigarrillo en el pulmón, y el papel protector de Omega 3 y la Vitamina D contra esos efectos. 28 ratas Wistar albino preñadas fueron separadas en cuatro grupos. El primer grupo grupo control; el segundo grupo estuvo expuesto al humo de 10 cigarrillos por dispositivo de inhalación 2 horas / día después de la preñez; el tercer grupo se expuso al humo del cigarrillo junto con Omega 3 (0,5 mg / kg / día) y el cuarto grupo se expuso al humo del cigarrillo junto con vitamina D (42 microgramos / kg / día). Secciones de tejido pulmonar de las ratas recién nacidas se tiñeron con Hematoxilina Eosina y tricrómico de Masson. Se midieron los niveles de malondialdehído (MDA) y productos de oxidación fluorescente (POF). Los pesos fetales y el número de fetos fueron significativamente más bajos en el grupo que recibió solamente humo de cigarrillo (ambos p <0,001). Histopatológicamente, el volumen pulmonar, el número de alveolos desarrollados y la elasticidad del parénquima disminuyeron significativamente; mientras que el tejido intersticial aumentó y la elastina y el colágeno no se desarrollaron adecuadamente. Los cambios histopatológicos disminuyeron significativamente en el grupo que recibió Omega 3 y Vitamina D. Estadísticamente, se encontró que los niveles de MDA y POF eran más altos en el grupo expuesto al humo de cigarrillo en comparación con el grupo control, además los niveles de MDA y POF fueron más bajos en el grupo que recibió Omega 3 junto con el humo del cigarrillo (p <0,001). El humo del cigarrillo causó daños histológicamente significativos en el tejido pulmonar fetal, el estrés oxidativo y el aumento de los niveles de MDA y FOU. Este daño se redujo significativamente con los suplementos de Omega 3 y Vitamina D. El omega 3 es un importante antioxidante; la vitamina D no tiene ningún efecto antioxidante significativo.

Animals , Female , Pregnancy , Rats , Vitamin D/administration & dosage , Fatty Acids, Omega-3/administration & dosage , Maternal Exposure/adverse effects , Lung Injury/prevention & control , Nicotine/toxicity , Smoke/adverse effects , Analysis of Variance , Rats, Wistar , Oxidative Stress , Lung Injury/chemically induced , Lung Injury/pathology , Fetus/drug effects , Fluorescence , Animals, Newborn , Malondialdehyde/analysis
J. bras. pneumol ; 41(4): 323-330, July-Aug. 2015. tab, ilus
Article in English | LILACS | ID: lil-759332


AbstractObjective: To evaluate HRCT scans of the chest in 22 patients with cocaine-induced pulmonary disease.Methods: We included patients between 19 and 52 years of age. The HRCT scans were evaluated by two radiologists independently, discordant results being resolved by consensus. The inclusion criterion was an HRCT scan showing abnormalities that were temporally related to cocaine use, with no other apparent causal factors.Results:In 8 patients (36.4%), the clinical and tomographic findings were consistent with "crack lung", those cases being studied separately. The major HRCT findings in that subgroup of patients included ground-glass opacities, in 100% of the cases; consolidations, in 50%; and the halo sign, in 25%. In 12.5% of the cases, smooth septal thickening, paraseptal emphysema, centrilobular nodules, and the tree-in-bud pattern were identified. Among the remaining 14 patients (63.6%), barotrauma was identified in 3 cases, presenting as pneumomediastinum, pneumothorax, and hemopneumothorax, respectively. Talcosis, characterized as perihilar conglomerate masses, architectural distortion, and emphysema, was diagnosed in 3 patients. Other patterns were found less frequently: organizing pneumonia and bullous emphysema, in 2 patients each; and pulmonary infarction, septic embolism, eosinophilic pneumonia, and cardiogenic pulmonary edema, in 1 patient each.Conclusions: Pulmonary changes induced by cocaine use are varied and nonspecific. The diagnostic suspicion of cocaine-induced pulmonary disease depends, in most of the cases, on a careful drawing of correlations between clinical and radiological findings.

ResumoObjetivo:Avaliar achados em TCAR de tórax de 22 pacientes com doença pulmonar induzida pelo uso de cocaína.Métodos:Foram incluídos pacientes com idades variando de 19 a 52 anos. As TCAR foram avaliadas por dois radiologistas, de forma independente, e os casos discordantes foram resolvidos por consenso. O critério de inclusão foi a presença de anormalidades na TCAR temporalmente relacionadas ao uso de cocaína, sem outros fatores causais justificáveis.Resultados:Oito pacientes (36,4%) apresentavam quadro clínico-tomográfico compatível com "pulmão de crack", e esses casos foram estudados separadamente. Os achados tomográficos predominantes nesse subgrupo de pacientes foram opacidades em vidro fosco, em 100% dos casos; consolidações, em 50%; e sinal do halo, em 25%. Em 12,5% dos casos, observou-se espessamento septal liso, enfisema parasseptal, nódulos centrolobulares e padrão de árvore em brotamento. Dentre os outros 14 pacientes (63,6%), observou-se barotrauma em 3 casos, apresentando-se como pneumomediastino, pneumotórax, e hemopneumotórax, respectivamente. Talcose foi diagnosticada em 3 pacientes, caracterizada como massas conglomeradas peri-hilares, distorção arquitetural e enfisema. Outros padrões encontrados com menor frequência foram pneumonia em organização e enfisema bolhoso, observados em 2 pacientes cada; e infarto pulmonar, embolia séptica, pneumonia eosinofílica e edema pulmonar cardiogênico, em 1 paciente cada.Conclusões:As alterações pulmonares induzidas pelo uso de cocaína são múltiplas e inespecíficas, e sua suspeita diagnóstica depende, na maioria dos casos, de uma cuidadosa correlação clínico-radiológica.

Adult , Female , Humans , Male , Middle Aged , Young Adult , Cocaine-Related Disorders , Cocaine/adverse effects , Lung Injury/chemically induced , Lung Injury , Brazil , Cocaine-Related Disorders/complications , Tomography, Spiral Computed
Rev. chil. enferm. respir ; 30(2): 95-99, jun. 2014. ilus, tab
Article in Spanish | LILACS | ID: lil-719129


Ammonia (NH3) is an irritant and corrosive gas whose inhalation at high concentrations mainly occurs during agricultural and industrial activities, as occupational accidents. The extent and severity of the damage depends on the concentration and time of exposure to the toxic, which can cause skin, eye, respiratory and life-threatening injuries. We present two cases of patients acutely exposed to high concentrations of NH3. Both patients survived to the acute phase of the respiratory injury, but developed chronic lung derangements.

El amoniaco (NH3) es un gas irritante y corrosivo cuya inhalación aguda en altas concentraciones se produce principalmente durante accidentes laborales en el sector agrícola e industrial. La extensión y severidad del daño depende de la concentración y tiempo de exposición al tóxico, el cual puede causar lesiones a nivel cutáneo, ocular, respiratorio y riesgo vital. Presentamos dos casos de pacientes expuestos en forma aguda a NH3 en altas concentraciones. Ambos pacientes sobrevivieron a la fase aguda y evolucionaron con lesiones respiratorias crónicas.

Humans , Male , Female , Adult , Bronchiectasis/chemically induced , Bronchiolitis/chemically induced , Lung Injury/chemically induced , Ammonia/adverse effects , Bronchi/injuries , Burns, Chemical/complications , Radiography, Thoracic , Accidents, Occupational , Tomography, X-Ray Computed , Occupational Diseases/chemically induced
Ann Card Anaesth ; 2013 Jul; 16(3): 188-192
Article in English | IMSEAR | ID: sea-147263


Aims and Objectives: We evaluated the incidence of coronary artery disease (CAD) in Sulfur mustard (SM) exposed veterans. We also evaluated the relationship between exposure to SM and angiography findings and compared angiography findings of SM exposed individuals with unexposed ones after two decades from the time of exposure to SM. Materials and Methods: A case-control study was conducted on 200 consecutive patients (100 SM exposed vs. 100 unexposed) undergoing angiographic assessments due to CAD. Results: The coronary angiography findings between two groups were significantly different ( P < 0.001). Ninety two (92%) patients in SM exposed group and 82 (82%) in unexposed group had abnormal findings in their coronary arteries ( P = 0.031). Conclusions: The incidence of CAD and angiographic changes were significantly increased with exposure to SM. Further studies on cardiovascular effects of SM are needed.

Chemical Warfare Agents/poisoning , Coronary Angiography , Coronary Disease/chemically induced , Coronary Disease/diagnostic imaging , Female , Humans , Iran , Lung Injury/chemically induced , Male , Middle Aged , Mustard Gas/poisoning , Veterans
The Korean Journal of Internal Medicine ; : 420-427, 2013.
Article in English | WPRIM | ID: wpr-212582


BACKGROUND/AIMS: Cyclophosphamide (CP) is a promising treatment for severe cases of paraquat (PQ) poisoning. We investigated the effective dose of CP for mitigating PQ-induced lung injury. METHODS: Adult male Sprague-Dawley rats were allocated into five groups: control, PQ (35 mg/kg, intraperitoneal injection), and PQ + CP (1.5, 15, or 30 mg/kg). The dimensions of lung lesions were determined using X-ray microtomography (micro-CT), and histological changes and cytokine levels were recorded. RESULTS: The micro-CT results showed that 15 mg/kg CP was more effective than 1.5 mg/kg CP for treating PQ-induced lung injury. At a dose of 1.5 mg/kg, CP alleviated the histological evidence of inflammation and altered superoxide dismutase activity. Using 15 mg/kg CP reduced the elevated catalase activity and serum transforming growth factor (TGF)-beta1 level. CONCLUSIONS: A CP dose of > 15 mg/kg is effective for reducing the severity of PQ-induced lung injury as determined by histological and micro-CT tissue examination, possibly by modulating antioxidant enzyme and TGF-beta1 levels.

Animals , Male , Rats , Catalase/metabolism , Cyclophosphamide/pharmacology , Cytokines/metabolism , Disease Models, Animal , Dose-Response Relationship, Drug , Immunosuppressive Agents/pharmacology , Inflammation Mediators/metabolism , Lung/drug effects , Lung Injury/chemically induced , Oxidative Stress/drug effects , Paraquat , Pulmonary Edema/chemically induced , Rats, Sprague-Dawley , Severity of Illness Index , Superoxide Dismutase/metabolism , Transforming Growth Factor beta1/metabolism , X-Ray Microtomography
Iran Occupational Health. 2011; 8 (1): 41-50
in Persian | IMEMR | ID: emr-145988


Ethylene oxide is used as a sterilizing agent in health care industries. The present study aimed to assess and recognize the nature of pulmonary reactions, if any, associated with occupational exposure to ethylene oxide and to investigate the prevalence of dermal, visual, neurologic, reproductive, hematologic, hepatic and renal disorders. Forty exposed and 47 unexposed employees were evaluated. Subjects were interviewed and standard respiratory symptom questionnaire as well as a questionnaire pertaining to symptoms of intoxication with this chemical were administered to them. Furthermore, parameters of pulmonary function were measured during exposure and a few days after exposure ceased. Additionally, blood samples were taken for CBC, liver and kidney function tests. moreover, atmospheric concentrations of ethylene oxide were determined by gas detector tubes. Respiratory symptoms such as cough and phlegm as well as dermal, visual and neurologic symptoms in exposed workers were significantly more prevalent p

Humans , Occupational Exposure , Lung Injury/chemically induced , Delivery of Health Care , Surveys and Questionnaires , Health Care Sector , Neurologic Manifestations
Experimental & Molecular Medicine ; : 525-537, 2011.
Article in English | WPRIM | ID: wpr-7975


Although 4,4'-diaminodiphenylsulfone (DDS, dapsone) has been used to treat several dermatologic conditions, including Hansen disease, for the past several decades, its mode of action has remained a topic of debate. We recently reported that DDS treatment significantly extends the lifespan of the nematode C. elegans by decreasing the generation of reactive oxygen species. Additionally, in in vitro experiments using non-phagocytic human fibroblasts, we found that DDS effectively counteracted the toxicity of paraquat (PQ). In the present study, we extended our work to test the protective effect of DDS against PQ in vivo using a mouse lung injury model. Oral administration of DDS to mice significantly attenuated the lung tissue damage caused by subsequent administration of PQ. Moreover, DDS reduced the local expression of mRNA transcripts encoding inflammation-related molecules, including endothelin-1 (ET-1), macrophage inflammatory protein-1alpha (MIP-1alpha), and transforming growth factor-beta (TGF-beta). In addition, DDS decreased the PQ-induced expression of NADPH oxidase mRNA and activation of protein kinase Cmicro (PKCmicro). DDS treatment also decreased the PQ-induced generation of superoxide anions in mouse lung fibroblasts. Taken together, these data suggest the novel efficacy of DDS as an effective protective agent against oxidative stress-induced tissue damages.

Animals , Male , Mice , Cells, Cultured , Chemokine CCL3/drug effects , Dapsone/administration & dosage , Endothelin-1/drug effects , Fibroblasts/drug effects , Herbicides/antagonists & inhibitors , Lung Injury/chemically induced , Mice, Inbred BALB C , Oxidative Stress , Paraquat/antagonists & inhibitors , Protective Agents/administration & dosage , Protein Kinase C/genetics , Superoxides/analysis , Transforming Growth Factor beta/drug effects
The Korean Journal of Internal Medicine ; : 95-100, 2009.
Article in English | WPRIM | ID: wpr-166675


BACKGROUND/AIMS: Paraquat-induced lung injury has been considered a progressive and irreversible disease. The purpose of this study was to report the long-term evolution of lung lesions in eight survivors with significant paraquat-induced lung injuries who could be followed-up for longer than 6 months. METHODS: We retrospectively examined high-resolution computed tomography and pulmonary function test of eight survivors with significant paraquat-induced lung injurys. RESULTS: High-resolution computed tomography revealed a predominant pattern of irregularly shaped consolidation with traction bronchiectasis at 1-2 months after paraquat poisoning, a mixed pattern of irregularly shaped consolidation and ground-glass opacity at 3-12 months, and a mixed pattern of consolidation, groundglass opacity, and honeycombing at 1-2 years. At 3-12 months after paraquat ingestion, the areas of consolidation had markedly decreased and the decreased lung volume had returned to normal. At 1-2 years after paraquat poisoning, the cystic changes had disappeared. At 2-3 years after paraquat poisoning, the decrease in forced vital capacity had greatly improved to the normal range. CONCLUSIONS: Recovery of nearly normal pulmonary structure and function may occur over several years following paraquat poisoning. Pulmonary function (both forced vital capacity and forced expiratory volume in 1 sec) evolved toward normal in the long-term survivors of paraquat poisoning with initial prominent lung injuries.

Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Bronchiectasis/chemically induced , Follow-Up Studies , Forced Expiratory Volume , Herbicides/toxicity , Lung/drug effects , Lung Injury/chemically induced , Lung Volume Measurements , Paraquat/toxicity , Pulmonary Fibrosis/chemically induced , Recovery of Function , Retrospective Studies , Survivors , Time Factors , Tomography, X-Ray Computed , Vital Capacity
Journal of Korean Medical Science ; : 636-640, 2009.
Article in English | WPRIM | ID: wpr-170159


Even though plasma paraquat (PQ) levels have known to be an informative predictor, many patients succumb at low PQ levels in acute PQ intoxication. This study was designed to see whether the high resolution computerized tomography (HRCT) of the lungs would be a predictive measure in acute PQ intoxication. HRCT of the lungs was obtained from 119 patients with acute PQ intoxication on 7 days after PQ ingestion. The areas with ground glass opacities (GGOs) were evaluated at five levels with the area measurement tool of the picture archiving and communication systems. Among 119 patients, 102 survived and 17 died. The plasma PQ levels were significantly higher in the non-survivors than in the survivors (2.6+/-4.0 microgram/mL vs. 0.2+/-0.4 microgram/mL, P=0.02). The area with GGOs was 2.0+/-6.4% in the survivors and 73.0+/- 29.9% in the non-survivors (P<0.001). No patients survived when the area with GGOs was more than 40% but all of the patients survived when the area affected by GGOs was less than 20%. In conclusion, the area of GGOs is a useful predictor of survival in acute PQ intoxication, especially in patients with low plasma PQ levels.

Adult , Female , Humans , Male , Middle Aged , Acute Disease , Diagnosis, Differential , Herbicides/blood , Lung Injury/chemically induced , Paraquat/blood , Predictive Value of Tests , Retrospective Studies , Survivors , Tomography, X-Ray Computed
J. bras. pneumol ; 34(7): 445-452, jul. 2008. ilus, tab
Article in English, Portuguese | LILACS | ID: lil-488269


OBJETIVO: Investigar os efeitos da quercetina em um modelo de inflamação pulmonar e fibrose induzidas por bleomicina. MÉTODOS: Setenta e nove hamsters machos adultos foram randomizados para aplicação de injeções pelas vias intratraqueal (IT) e intraperitoneal (IP) em quatro configurações: veículo IP/salina IT (grupo VS, n = 16); salina IT/quercetina IP (grupo QS, n = 16); bleomicina IT/veículo IP (grupo VB, n = 27); e bleomicina IT/quercetina IP (grupo QB, n = 20). A quercetina e a bleomicina foram aplicadas em doses de 30 mg/kg/dia e 10 U/kg, respectivamente.A quercetina foi iniciada/suspensa 3 dias antes/14 dias depois das injeções IT. RESULTADOS: A taxa de mortalidade do grupo VB foi significantemente superior à dos demais grupos (44 por cento vs. VS: 0 por cento; QS: 0 por cento; QB: 15 por cento). O grupo VB exibiu níveis pulmonares de substâncias reativas ao ácido tiobarbitúrico (× 10-2 nmol/mg) significativamente maiores (6,6 ± 1,3 vs. VS: 5,5 ± 0,8; QS: 2,5 ± 0,6; e QB: 5,8 ± 0,6).Os grupos VB/QB mostraram níveis pulmonares de glutationa reduzida (× 10-2 nmol/mg) significativamente menores que os dos grupos VS/QS (28,9 ± 13,8/28,6 ± 14,8 vs. 43,9 ± 16,0/51,1 ± 20,3) e níveis de hidroxiprolina (mg/g) significativamente maiores (201,6 ± 37,3/177,6 ± 20,3 vs. 109,6 ± 26,1/117,5 ± 32,0). CONCLUSÕES: Em um modelo animal de lesão pulmonar, a quercetina exibiu efeitos antiinflamatórios que são relacionados, pelo menos em parte, a suas propriedades antioxidantes.

OBJECTIVE: The aim of this study was to identify the best experimental model in which to observe the pulmonary alterations characterizing hepatopulmonary syndrome (HPS). METHODS: Male Wistar rats, with mean weight of 250 g, were used in four experimental models: inhaled carbon tetrachloride; intraperitoneal carbon tetrachloride; partial portal vein ligation; and bile duct ligation (BDL). The animals in all groups were divided into control and experimental subgroups. The following variables were measured: transaminase levels; blood gases; lipoperoxidation, using thiobarbituric acid reactive substances (TBARS) and chemiluminescence; and levels of superoxide dismutase (SOD) anti-oxidant activity. Anatomopathological examination of the lung was also performed. RESULTS: There were statistically significant differences between the BDL control and BDL experimental groups: aspartate aminotransferase (105.3 ± 43 vs. 500.5 ± 90.3 IU/L); alanine aminotransferase (78.75 ± 37.7 vs. 162.75 ± 35.4 IU/L); alkaline phosphatase (160 ± 20.45 vs. 373.25 ± 45.44 IU/L); arterial oxygen tension (85.25 ± 8.1 vs. 49.9 ± 22.5 mmHg); and oxygen saturation (95 ± 0.7 vs. 73.3 ± 12.07 percent). Lipoperoxidation and antioxidant activity also differed significantly between the two BDL groups (control vs. experimental): TBARS (0.87 ± 0.3 vs. 2.01 ± 0.9 nmol/mg protein); chemiluminescence (16008.41 ± 1171.45 vs. 20250.36 ± 827.82 cps/mg protein); and SOD (6.66 ± 1.34 vs. 16.06 ± 2.67 IU/mg protein). The anatomopathological examination confirmed pulmonary vasodilatation in the BDL model. In the other models, there were no alterations that were characteristic of HPS. CONCLUSIONS: The data obtained suggest that the BDL model can be used in future studies involving hepatic alterations related to oxidative stress and HPS.

Animals , Cricetinae , Male , Antioxidants/therapeutic use , Lung Injury/prevention & control , Oxidative Stress/drug effects , Pulmonary Fibrosis/prevention & control , Quercetin/therapeutic use , Analysis of Variance , Antibiotics, Antineoplastic , Antioxidants/administration & dosage , Bleomycin , Collagen/metabolism , Disease Models, Animal , Lipid Peroxidation/drug effects , Lung Injury/chemically induced , Lung/drug effects , Lung/pathology , Mesocricetus , Pulmonary Fibrosis/chemically induced , Pulmonary Fibrosis/pathology , Quercetin/administration & dosage , Random Allocation
Clinics ; 63(1): 77-84, 2008. ilus, graf, tab
Article in English | LILACS | ID: lil-474932


OBJECTIVE: To evaluate the effects of pentoxifylline on hydrochloric acid-induced lung lesions in rats subjected to mechanical ventilation. METHODS: Twenty male, adult Wistar-EPM-1 rats were anesthetized and randomly grouped (n=5 animals per group) as follows: control-MV (mechanical ventilation, MV group); bilateral instillation of HCl (HCl group); bilateral instillation of HCl followed by pentoxifylline (50 mg/kg bw) infusion (HCl+PTX group) and pentoxifylline infusion followed by bilateral instillation of HCl (PTX+HCl group). At 20, 30, 90 and 180 min after treatments, the blood partial pressures of CO2 and O2 were measured. The animals were euthanized, and bronchoalveolar lavages were taken to determine the contents of total proteins, corticosteroid and TNF-alpha. Samples of lung tissue were used for histomorphometric studies and determining the wet-to-dry (W/D) lung weight ratio. RESULTS: In the MV group, rats had alveolar septal congestion, and, in the HCl group, a remarkable recruitment of neutrophils and macrophages into the alveoli was noticed; these events were reduced in the animals with PTX+HCl. The partial pressure of oxygen increased in PTX+HCl animals (121±5 mmHg) as compared with the HCl (62±6 mmHg) and HCl+PTX (67±3 mmHg) groups within 30 minutes. TNF-alpha levels in bronchoalveolar lavage were significantly higher in the HCl group (458±50 pg/mL), reduced in the HCl+PTX group (329±45 pg/mL) and lowest in the PTX+HCl group (229±41 pg/mL). The levels of corticosteroid in bronchoalveolar lavage were significantly lower in the HCl (8±1.3 ng/mL) and HCl+PTX group (16±2 ng/mL) and were highest in the PTX+HCl (27±1.9 ng/mL). CONCLUSION: Pretreatment with PTX improves oxygenation, reduces TNF-alpha concentration and increases the concentration of corticosteroid in bronchoalveolar lavage upon lung lesion induced by HCl.

Animals , Male , Rats , Bronchoalveolar Lavage Fluid/chemistry , Lung Injury/pathology , Lung/pathology , Pentoxifylline/pharmacology , Tumor Necrosis Factor-alpha/drug effects , Adrenal Cortex Hormones/analysis , Blood Gas Analysis , Hydrochloric Acid , Instillation, Drug , Leukocyte Count , Lung Injury/chemically induced , Neutrophils/pathology , Proteins/analysis , Rats, Wistar , Respiration, Artificial
Rev. AMRIGS ; 51(4): 275-279, out.-dez. 2007. ilus, tab
Article in Portuguese | LILACS | ID: biblio-859916


Objetivo: Estudar o papel do probucol na lesão pulmonar obtida pela administração de doxorrubicina em ratos. Método: foi realizado um estudo piloto experimental, onde o probucol foi testado como protetor da injúria pulmonar obtida pela administração de doxorrubicina em ratos. Resultados: Na análise comparativa dos grupos, estudados por microscopia óptica, não houve diferença significativa de critérios previamente definidos, exceto pelo edema pleural (p < 0,05). Já na microscopia eletrônica, a agressão da doxorrubicina foi identificada através da desorganização estrutural. No grupo que recebeu probucol e doxorrubicina, não foi observada a mesma desorganização (p < 0,05). Conclusões: os resultados deste estudo piloto sugerem que o probucol exerceu um efeito protetor no tecido pulmonar agredido pela doxorrubicina e que a microscopia eletrônica é mais sensível na identificação de critérios de injúria pulmonar decorrente da exposição à doxorrubicina (AU)

Objective: To study the role of probucol in the pulmonary injury caused by doxorubicin in rats. Methods: An experimental study was carried out to verify where the probucol was protective of the pulmonary injury caused by the administration of doxorubicin in rats. Results: In the comparative analysis of the groups studied by optic microscopy, it did not have significant difference in pre-definite criterions, except for pleural edema (p < 0,05). In eletronic microscopy, the aggression of the doxorubicin was indicated through the structural disorganization. In the group that received probucol and doxorubicin was not observed the same disorganization (p < 0,05). Conclusion: The results suggest that the probucol was effective in the protection of pulmonary injury caused by doxorubicin and that the eletronic microscopy is more sensitive for pre-definite criterions of pulmonary injury (AU)

Animals , Male , Probucol/therapeutic use , Doxorubicin/toxicity , Lung Injury/chemically induced , Lung Injury/pathology , Sarcoma/secondary , Lipid Peroxidation/drug effects , Doxorubicin/antagonists & inhibitors , Lung/drug effects , Lung Neoplasms/secondary