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2.
Rev. chil. obstet. ginecol. (En línea) ; 83(3): 257-265, jun. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-959513

ABSTRACT

RESUMEN OBJETIVO: Establecer la utilidad diagnóstica de la relación neutrófilos/linfocitos en embarazadas con preeclampsia. MÉTODOS: Se realizó un estudio de casos y controles en el Hospital Central "Dr. Urquinaona", Maracaibo, Venezuela. Se seleccionó un total de 180 embarazadas. Se incluyeron 90 preeclámpticas como grupo de estudio (grupo A) y un grupo de control seleccionado por tener edad e índice de masa corporal similares al grupo de estudio, que consistió en 90 embarazadas normotensas sanas (grupo B). Se determinaron las características generales, valores de la relación neutrófilos/linfocitos y eficacia diagnóstica. RESULTADOS: Las pacientes del grupo A presentaron valores significativamente más altos de leucocitos y neutrófilos comparado con las pacientes del grupo B (p < 0,05). Por otra parte, las pacientes del grupo A presentaron valores más bajos de linfocitos comparado con las pacientes del grupo B (p < 0,001). Se encontraron diferencias estadísticamente significativas en la relación neutrófilos/linfocitos entre las pacientes del grupo A (4.0 +/- 1,3) comprado con las pacientes del grupo B (2,9 +/- 0,9; p < 0,001). Un valor de corte de la relación neutrófilos/linfocitos de 3,4 presentó un valor por debajo de la curva de 0,96, sensibilidad del 92,2%, especificidad del 87,8%, valor predictivo positivo del 88,3% y valor predictivo negativo del 91,9%, con una exactitud diagnóstica del 90,0%. CONCLUSIÓN: La relación neutrófilos/linfocitos es una herramienta útil en el diagnóstico de preeclampsia, ya que las pacientes con el síndrome presentan concentraciones significativamente más elevadas que las embarazadas controles normotensas.


ABSTRACT OBJECTIVE: To establish the diagnostic utility of the neutrophil/lymphocyte ratio in pregnant women with preeclampsia. METHODS: A case-control study was conducted at the Central Hospital "Dr. Urquinaona", Maracaibo, Venezuela. A total of 180 pregnant women were selected. We included 90 preeclamptic patients as a study group (group A) and a control group selected for having age and body mass index similar to the study group, which consisted of 90 healthy normotensive pregnant women (group B). The general characteristics, values of the neutrophil/lymphocyte ratio and diagnostic efficacy were determined. RESULTS: Patients in group A had significantly higher values of leukocytes and neutrophils compared to patients in group B (p < 0.05). On the other hand, patients in group A had lower lymphocyte values compared to patients in group B (p <0.001). Statistically significant differences were found in the neutrophil/lymphocyte ratio between patients in group A (4.0 +/- 1.3) compared with patients in group B (2.9 +/- 0.9, p <0.001). A cut-off value of the neutrophil/lymphocyte ratio of 3.4 showed a value under the curve of 0.96, sensitivity of 92.2%, specificity of 87.8%, positive predictive value of 88.3% and negative predictive value of 91.9%, with a diagnostic accuracy of 90.0%. CONCLUSION: The neutrophil/lymphocyte ratio is a useful tool in the diagnosis of preeclampsia, since the patients with the syndrome have significantly higher concentrations than the normotensive controls.


Subject(s)
Humans , Female , Pregnancy , Pre-Eclampsia/diagnosis , Lymphocytes/immunology , Neutrophils/immunology , Prognosis , Gestational Age , Maternal Age , Arterial Pressure
3.
Pesqui. vet. bras ; 36(12): 1155-1159, Dec. 2016. tab
Article in English | ID: biblio-842035

ABSTRACT

In order to investigate the immune enhancement effects of Ophiopogon japonicus polysaccharide Ophiopogon japonicus (OJPS) on Newcastle disease (ND) live vaccine, chickens vaccinated against ND live vaccine was orally administered with the OJPS at high, medium and low concentrations respectively. In negative control group, chickens were given orally equal volume of physiological saline. On day 14, 21 and 28, the serum antibody titer, erythrocyte-C3b receptor rosette rate (E-C3bRR), erythrocyte-C3b immune complex rosette rate (E-ICRR) and peripheral lymphocyte proliferation were measured. The results showed that at most time points, the antibody titer, peripheral lymphocyte proliferation, E-C3bRR and elimination rate of immune complex of three OJPS administrating groups were significantly higher (P<0.05) than those in negative control group. It indicated that OJPS could significantly improve the immune efficacy of Newcastle disease live vaccine, Ophiopogon japonicus polysaccharide possessed synergistical immunoenhancement.(AU)


Subject(s)
Animals , Chickens/virology , Newcastle Disease/immunology , Ophiopogon/chemistry , Viral Vaccines/analysis , Adjuvants, Immunologic , Antibodies/blood , Erythrocytes/immunology , Lymphocytes/immunology
4.
Rev. bras. reumatol ; 56(6): 497-503, Nov.-Dec. 2016. tab, graf
Article in English | LILACS | ID: biblio-830071

ABSTRACT

ABSTRACT Objective: To characterize the inflammatory profiles of patients with systemic lupus erythematosus receiving standard treatment compared to healthy controls. Patients and methods: Peripheral venous blood was collected from systemic lupus erythematosus patients (n = 14) and controls (n = 18) at enrollment. Blood samples were used for quantification, by flow cytometry, of CD11b (integrin) and Chemokine receptor CXCR2 expression surface antigen in neutrophils and lymphocytes, while cytokines were assayed in serum samples. Purified neutrophils were assayed by their ability to phagocytize human plasma-opsonized zymosan. Results: Patients had a median (interquartile range) disease activity index of 1.0 (0-2.0) characteristic of patients in remission. Interleukin-6 and interleukin-10 serum concentrations were significantly higher in the patient group compared to controls and the phagocytic index of circulating neutrophils was significantly reduced in patients compared to controls. The levels of interleukin-2, interleukin-5, interleukin-8 and tumor necrosis factor alpha did not significantly differ between patients and controls. Flow cytometric analysis revealed that the integrin expression levels were reduced in lymphocytes (but not in neutrophils) obtained from systemic lupus erythematosus patients, while surface expression of the chemokine receptor 2 was similar in both neutrophils and lymphocytes. Conclusion: Systemic lupus erythematosus patients receiving standard treatment presented with elevated systemic levels of interleukin-6 and interleukin-10, reduced neutrophil phagocytic capacity, and reduced lymphocyte expression of integrin even when symptoms were in remission. These alterations to innate immune components may put these individuals at a greater risk for acquiring infections.


RESUMO Objetivo: Caracterizar os perfis inflamatórios de pacientes com lúpus eritematoso sistêmico (LES) que recebiam o tratamento padrão em comparação com controles saudáveis. Pacientes e métodos: Coletou-se o sangue venoso periférico de pacientes com LES (n = 14) e controles (n = 18) no momento da entrada no estudo. As amostras de sangue foram usadas para quantificação, por citometria de fluxo, da expressão dos antígenos de superfície CD11b (integrina) e CXCR2 em neutrófilos e linfócitos, enquanto as citocinas foram avaliadas em amostras de soro. Avaliou-se a capacidade dos neutrófilos purificados de fagocitar zimosan opsonizado com plasma humano. Resultados: Os pacientes apresentavam uma pontuação mediana (intervalo interquartil) no Sledai de 1 (0-2), característica de pacientes em remissão. As concentrações séricas de IL-6 e IL-10 foram significativamente maiores no grupo de pacientes em comparação com os controles; o índice de fagocitose de neutrófilos circulantes estava significativamente reduzido nos pacientes em comparação com os controles. Os níveis de IL-2, IL-5, IL-8 e TNF-α não diferiram significativamente entre pacientes e controles. A análise da citometria de fluxo revelou que os níveis de expressão de CD11b estavam reduzidos nos linfócitos (mas não nos neutrófilos) obtidos de pacientes com LES, enquanto a expressão do receptor de superfície CXCR2 foi semelhante em neutrófilos e linfócitos. Conclusão: Os pacientes com LES que recebiam tratamento padrão apresentaram níveis sistêmicos elevados de IL-6 e IL-10, redução na capacidade fagocítica dos neutrófilos e redução da expressão de CD11b em linfócitos, mesmo quando os sintomas estavam em remissão. Essas alterações nos componentes da imunidade inata podem colocar esses indivíduos em maior risco de adquirir infecções.


Subject(s)
Humans , Lymphocytes/immunology , Cytokines/immunology , Lupus Erythematosus, Systemic/immunology , Neutrophils , Biomarkers/blood , Case-Control Studies , Interleukin-6
5.
Pesqui. vet. bras ; 36(4): 345-350, tab
Article in Portuguese | LILACS | ID: lil-787571

ABSTRACT

A gestação é um estado fisiológico que exige adaptações imunológicas para que transcorra normalmente. Nesse período a mãe e o feto apresentam uma relação imunológica, ou seja, a interface materno fetal. A enzima indoleamina 2,3 dioxigenase (IDO) desempenha um papel importante na tolerância materno fetal, por ser responsável pela metabolização do triptofano, impedindo por diversas vias a proliferação principalmente de linfócitos TCD8. Diversos tipos celulares estão presentes na interface materno fetal e vários deles podem expressar a IDO. Os leucócitos com perfil Th1 produzem uma citocina conhecida: o interferon γ que estimula a expressão da IDO em vários tipos celulares. Os linfócitos são divididos em subpopulações de acordo com sua função e fenótipo. Seus tipos incluem linfócitos T, linfócitos B e as células natural killer (NK). Hormônios também atuam nesse processo a progesterona que exerce função determinante sobre a resposta imunológica materna podendo alterar o prognóstico gestacional e o estrógeno essencial para a tolerância materno fetal e manutenção da prenhez. Dessa maneira este trabalho tem por objetivo principal identificar os linfócitos presentes na placenta bovina em cultivo que expressam IDO (linfócitos T, linfócitos B e células NK), frente a estimulação por progesterona, estrógeno e interferon γ nas diversas fases gestacionais utilizando a citometria de fluxo. Segundo os resultados no período de 67,5 a 77, 5 dias com a adição de interferon γ a expressão da enzima IDO aumentou discretamente nos linfócitos TCD3, TCD4, e diferente dos linfócitos T CD8 apresentaram uma elevada expressão da enzima (4,48 ± 2,12 - 8,65± 4,91)....


Pregnancy is a physiological state that requires immune adaptation in order to be successfully carried on. During this period, mother and fetus establish an immune tolerance status at the maternal fetal interface. Indoleamine 2,3-dioxygenase (IDO) plays an important role in maternal-fetal tolerance by metabolizing tryptophan, impairs by several pathways, mainly T CD8 cells proliferation. Several cell types are present in the maternal fetal interface and several of them can express IDO. Leucocytes with Th1 produce a cytokine known as interferon γ that stimulates the expression of IDO in several cell types. Lymphocytes are divided into sub-populations according to their function and phenotype: T lymphocytes, B lymphocytes and natural killer cells (NK). Hormones also involved in this process where progesterone exerts decisive role on maternal immune response that may change gestational outcome and estrogen is essential for fetal maternal tolerance and maintenance of pregnancy. Therefore, the main objective of this study was to identify lymphocytes in the bovine placental cell culture that are sensitive to progesterone, estrogen and interferon γ, IDO expression in various gestational stages using flow cytometry. According to the results in the gestational period from 67.5 to 77.5 days with the addition of interferon γ expression IDO was slightly increased in TCD3 lymphocytes, CD4, and differently from the other T cells CD8 displayed an higher expression of the enzyme (4.48±2.12 to 8.65±4.91)...


Subject(s)
Animals , Female , Pregnancy , Cattle , Immunophenotyping/veterinary , /analysis , Lymphocytes/classification , Lymphocytes/immunology , Placenta , Placenta/physiology , Immune Tolerance/physiology , B-Lymphocytes , Estrogens/analysis , Interferon-gamma/analysis , Killer Cells, Natural , Progesterone/analysis , T-Lymphocytes
6.
Braz. j. phys. ther. (Impr.) ; 20(2): 133-141, Mar.-Apr. 2016. tab, graf
Article in English | LILACS | ID: lil-783870

ABSTRACT

BACKGROUND: Therapeutic high-frequency ultrasound, microcurrent, and a combination of the two have been used as potential interventions in the soft tissue healing process, but little is known about their effect on the immune system. OBJECTIVE: To evaluate the effects of therapeutic high frequency ultrasound, microcurrent, and the combined therapy of the two on the size of the wound area, peritoneal macrophage function, CD4+ and CD8+, T lymphocyte populations, and plasma concentration of interleukins (ILs). METHOD: Sixty-five Wistar rats were randomized into five groups, as follows: uninjured control (C, group 1), lesion and no treatment (L, group 2), lesion treated with ultrasound (LU, group 3), lesion treated with microcurrent (LM, group 4), and lesion treated with combined therapy (LUM, group 5). For groups 3, 4 and 5, treatment was initiated 24 hours after surgery under anesthesia and each group was allocated into three different subgroups (n=5) to allow for the use of the different therapy resources at on days 3, 7 and 14 Photoplanimetry was performed daily. After euthanasia, blood was collected for immune analysis. RESULTS: Ultrasound increased the phagocytic capacity and the production of nitric oxide by macrophages and induced the reduction of CD4+ cells, the CD4+/CD8+ ratio, and the plasma concentration of IL-1β. Microcurrent and combined therapy decreased the production of superoxide anion, nitric oxide, CD4+-positive cells, the CD4+/CD8+ ratio, and IL-1β concentration. CONCLUSIONS: Therapeutic high-frequency ultrasound, microcurrent, and combined therapy changed the activity of the innate and adaptive immune system during healing process but did not accelerate the closure of the wound.


Subject(s)
Humans , Rats , Ultrasonic Therapy , Lymphocytes/immunology , Immune System/immunology , Wound Healing , Rats, Wistar
7.
Salvador; s.n; 2016. 88 p. ilus, tab.
Thesis in Portuguese | LILACS | ID: biblio-1001024

ABSTRACT

As leishmanioses constituem um complexo de doenças causada pelo protozoário intracelular, do gênero Leishmania, sendo a resposta imune celular essencial para controle, eliminação e proteção contra a infecção. A teoria clonal da imunidade celular propõe que as respostas imunológicas são estabelecidas através do aumento na frequência de clones específicos ao antígeno. Para avaliar a resposta das células T à infecção por Leishmania, investigamos, por citometria de fluxo, a expressão de cadeias Vβ de receptores de células T (TCRs), estado de ativação, capacidade de adesão ao endotélio e potencial funcional de clones específico. Em um grupo de pacientes com Leishmaniose Cutânea Localizada (LCL), avaliamos diferentes subpopulações de células T através da expressão da região Vβ, no sangue periférico e na biópsia da lesão. Utilizamos células mononucleares de sangue periférico (CMSPs), de pacientes LCL e controles saudáveis, nas quais avaliamos, ex vivo, a expressão de moléculas de ativação (CD25, CD69 e HLA-DR), adesão (LFA-1, VLA-4 e CD62L), co-estimulatória (CD27 e CD28)...


Leishmaniasis is a desease caused by infection with the Leishmania protozoan parasite. The cellular immune response is essential for controlling, eliminating and protection of the Leishmania infection. The clonal theory of cellular immunity proposes that immunological responses are established by increasing the frequency of antigen-specific clones. In order to measure the host T cell response to Leishmania infection, we have investigated by flow cytometry, the expression of Vβ chains of T-cell receptors (TCRs), activation state, adhesion to endothelium of capacity and functional potential of specific T. In a group of localized cutaneous leishmaniasis (LCL) patients, we evaluated different T cell subpopulations as identified by their Vβ region expression, in peripheral blood and biopsy. We used peripheral blood mononuclear cells (PBMCs), from CL patients and healthy volunteers, in which we evaluate, ex vivo, the expression of activation molecules (CD25, CD69 and HLA-DR), adhesion (LFA-1, VLA-4 and CD62L), co-stimulatory (CD27 and CD28)...


Subject(s)
Humans , Leishmaniasis, Cutaneous/diagnosis , Leishmaniasis, Cutaneous/epidemiology , Leishmaniasis, Cutaneous/parasitology , Leishmaniasis, Cutaneous/pathology , Leishmaniasis, Cutaneous/prevention & control , Lymphocytes/immunology , Lymphocytes/microbiology , Lymphocytes/pathology
8.
Rev. Soc. Bras. Med. Trop ; 48(5): 514-523, Sept.-Oct. 2015. tab, graf
Article in English | LILACS | ID: lil-763338

ABSTRACT

ABSTRACTINTRODUCTION:While no single factor is sufficient to guarantee the success of influenza vaccine programs, knowledge of the levels of immunity in local populations is critical. Here, we analyzed influenza immunity in a population from Southern Brazil, a region with weather conditions that are distinct from those in the rest of country, where influenza infections are endemic, and where greater than 50% of the population is vaccinated annually.METHODS:Peripheral blood mononuclear cells were isolated from 40 individuals. Of these, 20 had received the H1N1 vaccine, while the remaining 20 were unvaccinated against the disease. Cells were stimulated in vitro with the trivalent post-pandemic influenza vaccine or with conserved major histocompatibility complex I (MHC I) peptides derived from hemagglutinin and neuraminidase. Cell viability was then analyzed by [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide)]-based colorimetric assay (MTT), and culture supernatants were assayed for helper T type 1 (Th1) and Th2-specific cytokine levels.RESULTS:Peripheral blood lymphocytes from vaccinated, but not unvaccinated, individuals exhibited significant proliferation in vitro in the presence of a cognate influenza antigen. After culturing with vaccine antigens, cells from vaccinated individuals produced similar levels of interleukin (IL)-10 and interferon (IFN)-γ, while those from unvaccinated individuals produced higher levels of IFN-γ than of IL-10.CONCLUSIONS:Our data indicate that peripheral blood lymphocytes from vaccinated individuals are stimulated upon encountering a cognate antigen, but did not support the hypothesis that cross-reactive responses related to previous infections can ameliorate the immune response. Moreover, monitoring IL-10 production in vaccinated individuals could comprise a valuable tool for predicting disease evolution.


Subject(s)
Adult , Humans , Young Adult , Antibodies, Viral/immunology , Influenza A Virus, H1N1 Subtype/immunology , Influenza Vaccines/immunology , Influenza, Human/immunology , Lymphocytes/immunology , Antibodies, Viral/blood , Brazil/epidemiology , /immunology , Cross-Sectional Studies , Influenza, Human/epidemiology , Influenza, Human/prevention & control , Interferon-gamma/biosynthesis , /biosynthesis , Leukocytes, Mononuclear/immunology , Pandemics
9.
Article in English | IMSEAR | ID: sea-159279

ABSTRACT

Lymphoid malignancies (LM) are a heterogeneous group of disorders that are broadly divided into Hodgkin disease (HD) and Non-Hodgkin Lymphoma (NHL). Diagnosing lymphoid malignancies based on morphology in conjunction with immunohistochemistry (IHC) forms the basis of WHO classification and this has prognostic implications.With this background this study was designed thus including all the lymphoid malignancies both NHL and HD. Materials and Methods: This study was conducted at a tertiary centre in Uttarakhand and included a total of 116 cases of lymphoid malignancies. Of these 41 cases were of Hodgkin disease and 75 cases were of NHL. These cases were initially diagnosed on morphology employing Hematoxylin and Eosin (H&E) and special stains like Reticulin. Subsequently, a preliminary panel of monoclonal antibodies using CD3, CD15, CD20, CD30, and CD45 were employed. All the cases were then classified using WHO classification. Results: HD- Of the 41 cases of Hodgkin’s disease the commonest subtype was Nodular Sclerosis seen in 26 cases (48.78%). Reed Sternberg in reactive milieu is diagnostic of Hodgkin disease. In all cases except one Reed Sternberg cells exhibited positivity for both CD15 and CD30. NHL – Of the 75 cases of NHL an initial classification based on morphology was done. All the cases were classified according to International Working Formulation initially. Subsequently, IHC was employed using CD3, CD15, CD20 and CD45. The disease was then classified according to WHO classification and broadly divided into B or T cell types. B cell expression was seen in 60 cases (80%) and T cell expression in 15 cases (20%). The commonest B cell subtype was Diffuse Large B cell Lymphoma (26.4%).


Subject(s)
Adolescent , Adult , Child , Female , Hodgkin Disease/immunology , Humans , Immunohistochemistry/methods , Lymph Nodes/immunology , Lymphocytes/immunology , Lymphoma/immunology , Lymphoma, Non-Hodgkin/immunology , Male , Middle Aged , Young Adult
10.
Article in English | WPRIM | ID: wpr-210970

ABSTRACT

Mucosal immune responses against Pygidiopsis summa (Trematoda: Heterophyidae) infection were studied in ICR mice. Experimental groups consisted of group 1 (uninfected controls), group 2 (infection with 200 metacercariae), and group 3 (immunosuppression with Depo-Medrol and infection with 200 metacercariae). Worms were recovered in the small intestine at days 1, 3, 5, and 7 post-infection (PI). Intestinal intraepithelial lymphocytes (IEL), mast cells, and goblet cells were counted in intestinal tissue sections stained with Giemsa, astra-blue, and periodic acid-Schiff, respectively. Mucosal IgA levels were measured by ELISA. Expulsion of P. summa from the mouse intestine began to occur from days 3-5 PI which sustained until day 7 PI. The worm expulsion was positively correlated with proliferation of IEL, mast cells, goblet cells, and increase of IgA, although in the case of mast cells significant increase was seen only at day 7 PI. Immunosuppression suppressed all these immune effectors and inhibited worm reduction in the intestine until day 7 PI. The results suggested that various immune effectors which include IEL, goblet cells, mast cells, and IgA play roles in regulating the intestinal mucosal immunity of ICR mice against P. summa infection.


Subject(s)
Animals , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Goblet Cells/immunology , Heterophyidae/immunology , Immunity, Mucosal , Immunoglobulin A/analysis , Intestine, Small/parasitology , Leukocyte Count , Lymphocytes/immunology , Male , Mast Cells/immunology , Mice , Mice, Inbred ICR , Parasite Load , Time Factors , Trematode Infections/immunology
11.
Rev. Soc. Bras. Med. Trop ; 46(1): 73-78, Jan.-Feb. 2013. ilus, tab
Article in English | LILACS | ID: lil-666798

ABSTRACT

INTRODUCTION: This study evaluated the intracellular profile of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-10 (IL-10) and interferon-γ (IFN-γ) in peripheral blood mononuclear cells (PBMCs) from leprosy patients based on oral infections presence to determine whether these coinfections could be associated with pro-inflammatory activity in leprosy. METHODS:Leprosy patients regardless of clinical form and specific leprosy treatment (n=38) were divided into two groups: Group I - leprosy patients with oral infections (n=19), and Group II - leprosy patients without oral infections (n=19). Non-leprosy patients presenting oral infections were assigned to the control Group (n=10). Intracellular IL-2, IL-4, IL-10 and IFN-γ production was evaluated by flow cytometry (FACS) before and 7 days after controlling the oral infection in the Group I, before and 7 days after dental prophylaxis in the Group II, and during oral infection process in control Group. RESULTS: Low percentages of CD3+ lymphocytes bearing IL-2, IL-10 and IFN-γ were observed in the Group I and Group II at baseline and 7 days after therapy or prophylaxis compared to controls. Group I showed reduced percentages of IL-4 at baseline and 7 days after therapy compared to controls, or at baseline of Group II, and the Group II showed reduced percentages of CD3+ cells bearing IL-4 compared to control. An increase of the percentages of CD3+cells bearing IL-4 was observed in the Group I after the oral infections treatment. CONCLUSIONS: The occurrence of oral infections favors the intracellular cytokines expression and, probably, the inflammatory reaction operating as a stimulatory signal triggering the leprosy reactions.


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Coinfection/immunology , Cytokines/immunology , Leprosy/immunology , Lymphocytes/immunology , Periodontal Diseases/immunology , Case-Control Studies , Cytokines/blood , Interferon-gamma/blood , Interferon-gamma/immunology , /blood , /immunology , /blood , /immunology , /blood , /immunology , Leprosy/complications , Periodontal Diseases/complications
12.
Article in English | WPRIM | ID: wpr-103960

ABSTRACT

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated. The ORF of Gam56 gene was cloned into an eukaryotic expression vector pcDNA3.1(zeo)+. Expression of Gam56 protein in COS-7 cells transfected with recombinant plasmid pcDNA-Gam56 was confirmed by indirect immunofluorescence assay. The DNA vaccine was injected intramuscularly to yellow feathered broilers of 1-week old at 3 dosages (25, 50, and 100 microg/chick). Injection was repeated once 1 week later. One week after the second injection, birds were challenged orally with 5x10(4) sporulated oocysts of E. maxima, then weighed and killed at day 8 post challenge. Blood samples were collected and examined for specific peripheral blood lymphocyte proliferation activity and serum antibody levels. Compared with control groups, the administration of pcDNA-Gam56 vaccine markedly increased the lymphocyte proliferation activity (P<0.05) at day 7 and 14 after the first immunization. The level of lymphocyte proliferation started to decrease on day 21 after the first immunization. A similar trend was seen in specific antibody levels. Among the 3 pcDNA-Gam56 immunized groups, the median dosage group displayed the highest lymphocyte proliferation and antibody levels (P<0.05). The median dosage group had the greatest relative body weight gain (89.7%), and the greatest oocyst shedding reduction (53.7%). These results indicate that median dosage of DNA vaccine had good immunogenicity and immune protection effects, and may be used in field applications for coccidiosis control.


Subject(s)
Animals , Antibodies, Protozoan/blood , Antigens, Protozoan/genetics , Cell Proliferation , Chickens , Coccidiosis/immunology , Disease Models, Animal , Eimeria/genetics , Injections, Intramuscular , Lymphocytes/immunology , Protozoan Vaccines/administration & dosage , Vaccination/methods , Vaccines, DNA/administration & dosage
13.
Rev. Fac. Med. (Caracas) ; 35(2): 28-35, jul.-dic. 2012. tab, graf
Article in Spanish | LILACS | ID: lil-682984

ABSTRACT

El manejo nutricional de los pacientes infectados con el virus de inmunodeficiencia humana (VIH) es desafiante; especialmente cuando los recuentos de linfocitos T CD4 son < de 200 células/mm³, debido a los episodios de pérdida de peso que experimentan los pacientes. En este estudio se plantea demostrar la relación entre los niveles de carga del VIH y los valores de linfocitos T CD4+ con los cambios de peso corporal en los pacientes ambulatorios del Centro de Atención a Pacientes con Enfermedades Infectocontagiosas (CAPEI) de la Facultad de Odontología de la Universidad Central de Venezuela. La mayoría de los pacientes que presentaba pérdida de peso tuvo linfocitos TCD4 ‹ de 200 células/mm³. Los que mantuvieron el peso o lo ganaron, presentaron contajes de linfocitos TCD4 entre 200 y 499 células/mm³ (p = 0,012). Con relación con la carga viral se encontró que los pacientes que perdieron peso, cursaban en su mayoría, con valores ‹ 1000 o › 100.000 copias/ml. Al contrario, la mayor parte de los que aumentaron de peso, presentaron valores indetectables de carga viral. Estas diferencias no fueron estadísticamente significativas. Estos resultados tal vez reflejen el pronóstico de la pérdida de peso cuando el contaje de linfocitos TCD4 es ‹ de 200 células/mm³, ya que en este nivel ocurren infecciones oportunistas, favorecidas por la desnutrición, estableciéndose un círculo vicioso que aumentaría la morbi mortalidad. Evaluar el estado inmunológico – nutricional en pacientes ambulatorios con infección por VIH es importante para iniciar estrategias encaso de pérdida de peso corporal


The nutritional management of patients infected with the human immunodeficiency (HIV) virus is challenging; especially when linfocitos T CD4 counts are < of 200 cells/mm³, due to episodes of weight loss experienced patients. This study raises show the relationship between the levels of the HIV load and the values of T CD4 + lymphocytes with changes in body weight in the outpatient care center for patients with diseases transmittable-contagious (CAPEI) of the Faculty of Dentistry of the University Central of Venezuela. Most of the patients who had weight loss had CD4 cell count of ‹ 200 cells/mm³ Those who maintained the weight or won it, presented between 200 and 499 CD4 cell counts cells/mm³ (p = 0.012). In relation with the viral load was found that patients who lost weight, were mostly with values 1000 ‹ or › 100,000 copies/ml. On the contrary, most of those who gained weight, they were undetectable viral load values. These differences were not statistically significant. These results may reflect the prognosis of weight loss when the CD4 cell count is ‹ 200 cells/mm³, since at this level occur opportunistic infections, favored by malnutrition, establishing a vicious circle that would increase the morbidity mortality. Assess the immune status - nutrition in outpatients with HIV infection is important to initiate strategies in the event of loss of body weight


Subject(s)
Humans , Male , Female , Body Mass Index , HIV , Lymphocytes/immunology , Weight Loss/immunology , Receptors, HIV , Viremia/immunology , Allergy and Immunology
14.
J. bras. pneumol ; 38(3): 321-330, maio-jun. 2012. ilus, tab
Article in Portuguese | LILACS | ID: lil-640755

ABSTRACT

OBJETIVO: Investigar o significado de marcadores de imunidade celular e de componentes elásticos/colágeno da matriz extracelular em estruturas granulomatosas em biópsias de pacientes com sarcoidose pulmonar ou extrapulmonar. MÉTODOS: Determinações qualitativas e quantitativas de células inflamatórias, de fibras de colágeno e de fibras elásticas em estruturas granulomatosas em biópsias cirúrgicas de 40 pacientes com sarcoidose pulmonar e extrapulmonar foram realizadas por histomorfometria, imuno-histoquímica, e técnicas de coloração com picrosirius e resorcina-fucsina de Weigert. RESULTADOS: A densidade de linfócitos, macrófagos e neutrófilos nas biópsias extrapulmonares foi significativamente maior do que nas biópsias pulmonares. Os granulomas pulmonares apresentaram uma quantidade significativamente maior de fibras de colágeno e menor densidade de fibras elásticas que os granulomas extrapulmonares. A quantidade de macrófagos nos granulomas pulmonares correlacionou-se com CVF (p < 0,05), ao passo que as quantidades de linfócitos CD3+, CD4+ e CD8+ correlacionaram-se com a relação VEF1/CVF e com CV. Houve correlações negativas entre CPT e contagem de células CD1a+ (p < 0,05) e entre DLCO e densidade de fibras colágenas/elásticas (r = -0,90; p = 0,04). CONCLUSÕES: A imunofenotipagem e o remodelamento apresentaram características diferentes nas biópsias dos pacientes com sarcoidose pulmonar e extrapulmonar. Essas diferenças correlacionaram-se com os dados clínicos e espirométricos dos pacientes, sugerindo que há duas vias envolvidas no mecanismo de depuração de antígenos, que foi mais eficaz nos pulmões e linfonodos.


OBJECTIVE: To investigate the significance of cellular immune markers, as well as that of collagen and elastic components of the extracellular matrix, within granulomatous structures in biopsies of patients with pulmonary or extrapulmonary sarcoidosis. METHODS: We carried out qualitative and quantitative evaluations of inflammatory cells, collagen fibers, and elastic fibers in granulomatous structures in surgical biopsies of 40 patients with pulmonary and extrapulmonary sarcoidosis using histomorphometry, immunohistochemistry, picrosirius red staining, and Weigert's resorcin-fuchsin staining. RESULTS: The extrapulmonary tissue biopsies presented significantly higher densities of lymphocytes, macrophages, and neutrophils than did the lung tissue biopsies. Pulmonary granulomas showed a significantly higher number of collagen fibers and a lower density of elastic fibers than did extrapulmonary granulomas. The amount of macrophages in the lung samples correlated with FVC (p < 0.05), whereas the amount of CD3+, CD4+, and CD8+ lymphocytes correlated with the FEV1/FVC ratio and VC. There were inverse correlations between TLC and the CD1a+ cell count (p < 0.05), as well as between DLCO and collagen/elastic fiber density (r = -0.90; p = 0.04). CONCLUSIONS: Immunophenotyping and remodeling both showed differences between pulmonary and extrapulmonary sarcoidosis in terms of the characteristics of the biopsy samples. These differences correlated with the clinical and spirometric data obtained for the patients, suggesting that two different pathways are involved in the mechanism of antigen clearance, which was more effective in the lungs and lymph nodes.


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Extracellular Matrix/immunology , Immunity, Cellular , Immunophenotyping/methods , Sarcoidosis/immunology , Analysis of Variance , Biopsy , Collagen/immunology , Elastic Tissue/immunology , Elastic Tissue/pathology , Extracellular Matrix/pathology , Granuloma, Respiratory Tract/immunology , Granuloma, Respiratory Tract/pathology , Lung/immunology , Lung/pathology , Lymph Nodes/immunology , Lymph Nodes/pathology , Lymphocytes/immunology , Macrophages, Alveolar/immunology , Sarcoidosis, Pulmonary/immunology , Sarcoidosis, Pulmonary/pathology , Sarcoidosis/pathology
15.
Braz. dent. j ; 23(4): 322-327, 2012. ilus, tab
Article in English | LILACS | ID: lil-658005

ABSTRACT

It was assessed the immunohistochemical profile of CD25+ cells in cases of chronic gingivitis (CG) and chronic periodontitis (CP). Immunohistochemistry was carried out using streptoavidin-biotin complex and anti-CD25 antibody in 17 cases of CG and 25 cases of CP. Sixteen cases (94.1%) of CG were immunopositive. CD25 was focally expressed in 50% of the sample and diffusely expressed in 25%. The stained cells were localized not only beneath the epithelium, but also far from it. In relation to the cellular density quantification of CD25+ cells, score ++ was the most common. Concerning CP, all cases were immunopositive. CD25+ cells were expressed in focal or diffuse pattern either close or far from the epithelium. Diffuse distribution of positive cells throughout the connective tissue was seen in 60% of the cases and 32% showed focal or diffuse cellular pattern. Sixteen cases (64%) received score +++. It was identified that CD25+ cells are present in either a focal or a diffuse pattern in connective tissue. Significant differences in the density of cellular immunostaining between CG and CP were found. The greatest density was observed in CP cases, which suggests that the infiltrate of lymphocytes show a higher degree of cellular activation in periodontitis compared with gingivitis.


Foi avaliado o perfil imunohistoquímico das células CD25+ em casos de gengivite (CG) e periodontite crônica (CP). A imunohistoquímica foi realizada utilizando o complexo de streptoavidina-biotina e o anticorpo anti-CD25 em 17 casos de CG e 25 casos de CP. 16 casos (94.1%) de CG foram imunopositivos. O CD25 foi expresso focalmente em 50% da amostra e difusamente em 25% dos casos. As células imunomarcadas estavam localizadas não apenas no epitélio, mas também por todo o tecido conjuntivo. Em relação à quantificação da densidade celular de células CD25+, o escore ++ foi o mais comum. Em relação a CP, todos os casos foram imunopositivos. As células CD25+ foram expressas em padrão ora focal ora difuso, tanto no epitélio como no conjuntivo. A distribuição difusa das células positivas apenas no tecido conjuntivo foi observada em 60% dos casos, e 32% dos casos exibiram padrão celular ora focal ora difuso. 16 casos (64%) foram considerados como escore +++. Identificamos que as células CD25+ estão presentes em padrão ora focal ora difuso no tecido conjuntivo. Diferenças significantes na densidade da imunomarcação celular entre CG and CP foram encontradas. A maior densidade celular foi observada na periodontite, sugerindo que o infiltrado de linfócitos mostrou um maior grau de ativação celular na periodontite comparada à gengivite.


Subject(s)
Humans , Chronic Periodontitis/immunology , Gingivitis/immunology , /analysis , Cell Count , Chronic Disease , Connective Tissue Cells/immunology , Disease Progression , Epithelial Cells/immunology , Fibroblasts/immunology , Immunohistochemistry , Lymphocytes/immunology , Plasma Cells/immunology , T-Lymphocytes, Helper-Inducer/immunology
16.
Recife; s.n; 2012. 75 p. ilus, graf, tab, ^c30 cm.
Thesis in Portuguese | LILACS | ID: lil-638863

ABSTRACT

As interações moleculares entre os monócitos e os linfócitos são de extrema importância para a produção de uma resposta imune eficiente. Nesse contexto nos propomos avaliar a expressão da molécula de superfície HLA-DR+ em monócitos CD14+, e das moléculas co-estimulatórias CD80+ e CD86+ e a produção das citocinas IL-10, IFN-gama, TNF-alfa e IL-12 por monócitos CD14+ HLA-DR+ de portadores de formas clínicas crônicas da doença de Chagas. O grupo de indivíduos acometidos cronicamente pela infecção chagásica foi composto por 10 portadores da forma cardíaca (FC) sem dilatação cardíaca (FC1), 14 portadores da forma cardíaca com dilatação cardíaca (FC2) e 7 portadores da forma indeterminada (FI). O sangue total destes indivíduos foi submetido à cultura na presença dos antígenos CRA ou FRA, específicos ao Trypanosoma cruzi. Observamos que o aumento na expressão da molécula co-estimulatória CD80+ associado à diminuição da expressão de CD86+ no grupo de indivíduos FC, quando comparamos os contextos ex vivo e após estímulo com CRA ou FRA, aponta um possível envolvimento desses antígenos nos mecanismos de fuga do sistema imune do hospedeiro pelo T. cruzi. Correlações positivas entre citocinas inflamatórias e anti-inflamatórias foram observadas entre os portadores da FI e FC1, indicando um controle do parasitismo, concomitantemente a uma modulação da resposta inflamatória. No grupo de pacientes FC2 observamos uma predominância de uma correlação positiva entre as citocinas inflamatórias, bem como de correlação positiva entre citocinas Th1 e Th2, indicando que há predomínio de um perfil do tipo Th1, mas com indícios de mecanismo de regulação da resposta inflamatória exacerbada. Nossos resultados sugerem que os antígenos CRA e FRA, por estarem presentes no parasito, poderiam atuar no processo de apresentação e ativação celular durante a cronicidade da doença, sobretudo nos indivíduos portadores da FC.


Subject(s)
Chagas Disease/immunology , Biomarkers , Monocytes/immunology , Recombinant Proteins , Trypanosoma cruzi/immunology , Antigens, Protozoan/immunology , Cell Culture Techniques , Lymphocytes/immunology , Tumor Necrosis Factor-alpha
17.
Rio de Janeiro; s.n; 2011. 111 p. ilus, tab.
Thesis in Portuguese | LILACS | ID: lil-613885

ABSTRACT

A L-aginina é reconhecida como um nutriente de fundamental importância na resposta imune, apesar de seus efeitos serem, por vezes, considerados inconstantes. O autoimplante esplênico tem sido proposto como alternativa à esplenectomia total isolada, mas existem preocupações quanto à eficácia do restabelecimento da resposta imune, haja vista que o paciente pode permanecer com risco aumentado de desenvolvimento de infecção fulminante pós esplenectomia, mesmo após a regeneração morfológica do órgão. O objetivo deste estudo foi avaliar a participação da suplementação dietética com L-arginina em subpopulações linfocitárias no sangue, no baço e nos autoimplantes esplênicos de ratos submetidos a esplenectomia isolada ou combinada com autoimplante esplênico. Foram utilizados 42 ratos Sprague-Dawlay machos, randomicamente distribuídos em seis grupos: 1 - Controle - operação simulada; 2 - esplenectomia total; 3 - esplenectomia total combinada com autoimplante esplênico; 4 - Controle - operação simulada, com suplementação de L-arginina; 5 - esplenectomia total, com suplementação de L-arginina; e 6 - esplenectomia total combinada com autoimplante esplênico, com suplementação de L-arginina. Os animais dos grupos 4, 5 e 6 receberam suplementação de L-arginina, uma vez ao dia, durante 15 dias anteriores a coleta sangüínea realizada imediatamente antes dos procedimentos operatórios (semanas 0 e 12). A dose utilizada foi de 1,0 g/kg/dia, administrada por via intragástrica em bolus. As avaliações foram realizadas por meio de hemograma e citometria de fluxo. A análise estatística utilizou testes paramétricos e não-paramétricos, sendo p<0,05 considerado para a rejeição da hipótese nula. A suplementação com L-arginina acarretou elevação da contagem relativa e absoluta de neutrófilos periféricos, 12 semanas após a realização de esplenectomia total combinada com autoimplante esplênico. A esplenectomia total ocasionou diminuição da contagem relativa de linfócitos T totais, T CD4+...


L-arginine is recognized as a nutrient of fundamental importance in immune implants has been proposed as an alternative to total splenectomy isolated, but there are concerns about the effectiveness of the restoration of the immune response, considering that the patient can remain at increased risk of developing overwhelming postsplenectomy infection, even after morphological regeneration of the organ. The aim of this study was to determine the role of dietary supplementation with L-arginine in lymphocyte subsets in blood, spleen, and splenic auto-transplantation in rats subjected to total splenectomy alone or in combination with splenic auto-transplantation. Forty two male Sprague-Dawley rats, were randomly divided into six groups: 1 - Control - sham operation, 2 - total splenectomy, 3 - total splenectomy combined with splenic auto-implants, 4 - Control - sham operation, with L-arginine supplementation, 5 - total splenectomy, supplemented with L-arginine, and 6 - total splenectomy combined with splenic auto-implants, supplemented with L-arginine. Animals in groups 4, 5 and 6 were supplemented with L-arginine, once daily for 15 days before blood sample was collected immediately before the operative procedures (weeks 0 and 12). The dose was 1.0 g/kg/day administered by intragastric bolus. The laboratory evaluations were made by blood count and flow cytometry. Statistical analysis used parametric tests and nonparametric, p<0.05 was considered to reject the null hypothesis. Supplementation with L-arginine led to increase in relative and absolute count of peripheral neutrophils, 12 weeks after completion of total splenectomy combined with splenic auto-implants. Total splenectomy caused a decrease in relative count of T lymphocytes, CD4+ and CD8B in blood, but dietary supplementation with L-arginine prevented the decrease in the percentage of total T cells and CD8B in the blood of animals subjected to splenic auto-transplantation...


Subject(s)
Rats , Arginine/administration & dosage , Arginine/immunology , Spleen/immunology , Spleen/transplantation , Splenectomy/methods , Immune System , Lymphocyte Subsets , Lymphocyte Subsets/immunology , Dietary Supplements , Transplantation, Autologous , Infections/etiology , Lymphocytes/immunology
18.
Iranian Journal of Parasitology. 2011; 6 (1): 34-40
in English | IMEMR | ID: emr-103781

ABSTRACT

Immunological response of host and parasite play a key role in developing vaccination and immunization. The present study deals with the immune response and effecter mechanism, which was confirmed by migration inhibition factor [MIF]. The present work was conducted in Parasitological Lab of Postgraduate Department of Zoology, Government Holkar Science College, Indore [M.P.] during 2006-2007. For MIF assay, lymphocytes were separated from heparinized blood of experimental and control mice. Aliquots of cell suspension were placed in four wells cut in a preparation of agarose in a Petri dish. Two wells were filled with soluble test antigen, while rest two wells were filled with medium [control wells]. Petri dish was incubated overnight at 37°C in a humidified environment at 5% CO2 in air. Cells migrated under the agarose in a circle were fixed and stained. Diameters of the migration areas were measured with ocular micrometer. MIF reaction was maximum [44.2%] in the group IVEgESAg5 and minimum [10.8%] in the group IVASoAg1. The maximum MIF reaction was shown by eggs ES antigen and least by adult worm somatic antigen. The interesting observation was that migration inhibition increases as dose increased or we could say the reaction was dose dependent Increased value of MIF response in vaccinated mice suggested the involvement of lymphocytes in cell-mediated immunity. This study also proves that excretory-secretory [ES] antigen of eggs from Trichuris muris was more effective in imparting immunity in mice


Subject(s)
Female , Animals, Laboratory , Lymphocytes/immunology , Cell Migration Inhibition , Trichuriasis , Mice , Vaccination , Antigens, Helminth
19.
Article in English | WPRIM | ID: wpr-131144

ABSTRACT

BACKGROUND: Since the recent introduction of radioimmunotherapy (RIT) using antibodies against cluster of differentiation (CD) 45 for the treatment of lymphoma, the clinical significance of the CD45 antigen has been increasing steadily. Here, we analyzed CD45 expression on lymphocyte subsets using flow cytometry in order to predict the susceptibility of normal lymphocytes to RIT. METHODS: Peripheral blood specimens were collected from 14 healthy individuals aged 25-54 yr. The mean fluorescence intensity (MFI) of the cell surface antigens was measured using a FACSCanto II system (Becton Dickinson Bioscience, USA). MFI values were converted into antibody binding capacity values using a Quantum Simply Cellular microbead kit (Bangs Laboratories, Inc., USA). RESULTS: Among the lymphocyte subsets, the expression of CD45 was the highest (725,368+/-42,763) on natural killer T (NKT) cells, 674,030+/-48,187 on cytotoxic/suppressor T cells, 588,750+/-48,090 on natural killer (NK) cells, 580,211+/-29,168 on helper T (Th) cells, and 499,436+/-21,737 on B cells. The Th cells and NK cells expressed a similar level of CD45 (P=0.502). Forward scatter was the highest in NKT cells (P<0.05), whereas side scatter differed significantly between each of the lymphocyte subsets (P<0.05). CD3 expression was highest in the Th and NKT cells. CONCLUSIONS: NKT cells express the highest levels of CD45 antigen. Therefore, this lymphocyte subset would be most profoundly affected by RIT or pretargeted RIT. The monitoring of this lymphocyte subset during and after RIT should prove helpful.


Subject(s)
Adult , Antibodies/immunology , Leukocyte Common Antigens/analysis , B-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Female , Flow Cytometry/methods , Fluorescein-5-isothiocyanate/chemistry , Fluorescent Dyes/chemistry , Humans , Killer Cells, Natural/immunology , Lymphocytes/immunology , Lymphoma/radiotherapy , Male , Middle Aged , Natural Killer T-Cells/immunology , Protein Binding , Radioimmunotherapy , Reagent Kits, Diagnostic , T-Lymphocytes, Helper-Inducer/immunology
20.
Article in English | WPRIM | ID: wpr-131141

ABSTRACT

BACKGROUND: Since the recent introduction of radioimmunotherapy (RIT) using antibodies against cluster of differentiation (CD) 45 for the treatment of lymphoma, the clinical significance of the CD45 antigen has been increasing steadily. Here, we analyzed CD45 expression on lymphocyte subsets using flow cytometry in order to predict the susceptibility of normal lymphocytes to RIT. METHODS: Peripheral blood specimens were collected from 14 healthy individuals aged 25-54 yr. The mean fluorescence intensity (MFI) of the cell surface antigens was measured using a FACSCanto II system (Becton Dickinson Bioscience, USA). MFI values were converted into antibody binding capacity values using a Quantum Simply Cellular microbead kit (Bangs Laboratories, Inc., USA). RESULTS: Among the lymphocyte subsets, the expression of CD45 was the highest (725,368+/-42,763) on natural killer T (NKT) cells, 674,030+/-48,187 on cytotoxic/suppressor T cells, 588,750+/-48,090 on natural killer (NK) cells, 580,211+/-29,168 on helper T (Th) cells, and 499,436+/-21,737 on B cells. The Th cells and NK cells expressed a similar level of CD45 (P=0.502). Forward scatter was the highest in NKT cells (P<0.05), whereas side scatter differed significantly between each of the lymphocyte subsets (P<0.05). CD3 expression was highest in the Th and NKT cells. CONCLUSIONS: NKT cells express the highest levels of CD45 antigen. Therefore, this lymphocyte subset would be most profoundly affected by RIT or pretargeted RIT. The monitoring of this lymphocyte subset during and after RIT should prove helpful.


Subject(s)
Adult , Antibodies/immunology , Leukocyte Common Antigens/analysis , B-Lymphocytes/immunology , CD8-Positive T-Lymphocytes/immunology , Female , Flow Cytometry/methods , Fluorescein-5-isothiocyanate/chemistry , Fluorescent Dyes/chemistry , Humans , Killer Cells, Natural/immunology , Lymphocytes/immunology , Lymphoma/radiotherapy , Male , Middle Aged , Natural Killer T-Cells/immunology , Protein Binding , Radioimmunotherapy , Reagent Kits, Diagnostic , T-Lymphocytes, Helper-Inducer/immunology
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