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1.
Rev. colomb. cir ; 36(3): 438-445, 20210000. tab
Article in Spanish | LILACS | ID: biblio-1254238

ABSTRACT

Introducción. La mastitis granulomatosa crónica es una enfermedad inflamatoria poco frecuente y con mayor incidencia en el sexo femenino. Su sintomatología y su presentación clínica causan gran ansiedad tanto en el paciente como en el personal médico, debido a su comportamiento similar al de la patología mamaria maligna. No hay una etiología clara ni un manejo terapéutico definido. El objetivo de este estudio fue determinar las características clínico-patológicas, el tratamiento y la evolución de las pacientes con mastitis granulomatosa, durante el periodo de estudio. Métodos. Estudio retrospectivo en el que se revisaron las historias clínicas de pacientes con diagnóstico y manejo de trastorno inflamatorio de la mama no especificado (N61X), entre enero de 2010 y diciembre de 2019. Se encontraron 236 pacientes, se excluyeron 176 por no cumplir con el diagnóstico de mastitis granulomatosa crónica o por no tener un seguimiento adecuado. Se evaluaron las características sociodemográficas, clínicas y de evolución, comparando la respuesta que se obtuvo con cada tratamiento. Resultados. Se incluyeron 60 pacientes femeninas que presentaron manifestaciones variadas. El 38,3 % (n=23) recibieron manejo antibiótico, el 30 % (n=18) fue tratado con corticoides, el 8,3 % (n=5) recibió antibióticos más corticoides y se realizó manejo expectante en el 16,6 % (n=10). El 6,6 % (n=4) de los pacientes fueron llevados a cirugía. Discusión. La mejor respuesta y la menor tasa de recidiva se encontró en las pacientes que fueron sometidas a observación y en aquellas que recibieron corticoides


Introduction. Chronic granulomatous mastitis is a rare inflammatory disease with a higher incidence in females. Its symptoms and its clinical presentation cause great anxiety both in the patient and in the medical personnel, due to its behavior similar to that of malignant breast disease. There is no clear etiology or defined therapeutic management. The objective of this study was to determine the clinical-pathological characteristics, treatment and evolution of the patients with granulomatous mastitis, during the study period. Methods. Retrospective study in which the medical records of patients with diagnosis and management of unspecified inflammatory disorder of the breast (N61X) were reviewed, between January 2010 and December 2019. Two-hundred-thirty-six patients were found, 176 were excluded for not complying with the diagnosis of chronic granulomatous mastitis or for not having an adequate follow-up. The sociodemographic, clinical and evolution characteristics were evaluated, comparing the response obtained with each treatment. Results. Sixty female patients who presented varied manifestations were included, of which 38.3% (n=23) received antibiotic treatment, 30% (n=18) were treated with steroids, 8.3% (n=5) received antibiotics plus steroids, expectant management was performed in 16, 6% (n = 10), and 6.6% (n=4) of the patients were taken to surgery.Discussion. The best response and the lowest recurrence rate were found in patients who were observed and in those who received steroids


Subject(s)
Humans , General Surgery , Mastitis , Adrenal Cortex Hormones , Granulomatous Mastitis , Fibrocystic Breast Disease , Anti-Bacterial Agents
2.
Rev. méd. Minas Gerais ; 31: 31408, 2021.
Article in Portuguese | LILACS | ID: biblio-1291383

ABSTRACT

A mastite é uma patologia relativamente frequente na mulher que amamenta. Surge maioritariamente nas primeiras seis semanas após o parto (prevalência entre 75-95% antes do bebé completar os três meses de vida), podendo, contudo, ocorrer ao longo de todo o período da amamentação. Apresenta-se clinicamente com mastalgia, eritema e edema mamário, linfonodomegalia axilar, febre, calafrios, mal-estar e prostração. Entretanto, o quadro clínico completo pode não estar presente em todos os casos. A técnica incorreta de amamentação provoca as microlesões mamáriasque são fatores associados à mastite, inicialmente, e esta quando não recebe tratamento adequado pode evoluirpara os abscessos mamários, que são caracterizados clinicamente por nodulação palpável ou não, flutuante, parcialmente circunscrita, geralmente no quadrante superior lateral da mama afetada. O microrganismo mais envolvido é o Staphylococcus aureus. O diagnóstico diferencial envolve principalmente outras causas não infecciosas de mastite, por exemplo a granulomatosa com reação a corpo estranho (piercing, implantes de silicone) e também malignidade. A ultrassonografia é um método bastante útil na investigação inicial daqueles quadros com suspeita de desenvolvimento de tal complicação e possibilita de maneira segura o tratamento minimamente invasivo e a obtenção de amostra para analise laboratorial. Isto permite a escolha de antibioticoterapia direcionada para os microorganismos causadores.


Mastitis is a relatively common condition in breastfeeding women. It appears mostly in the first six weeks after delivery (prevalence between 75-95% before the baby is three months old), however, it can occur throughout the entire period of breastfeeding. It may present clinically with mastalgia, erythema and breast edema, axillary lymph node enlargement, fever, chills, malaise and prostration. However, the complete clinical picture may not be present in all cases. The incorrect breastfeeding technique causes breast microlesions which are factors associated with mastitis, initially, and when it does not receive adequate treatment it can evolve for breast abscesses, which are characterized clinically by palpable or not, floating, partially circumscribed nodulation, usually in the upper lateral quadrant of the affected breast. The most involved microorganism is Staphylococcus aureus. The differential diagnosis mainly involves other non-infectious causes of mastitis, for example granulomatous with a foreign body reaction (piercing, silicone implants) and also malignancy. Ultrasonography is a very useful method in the initial investigation of those conditions suspected of developing such a complication, and it safely allows minimally invasive treatment and obtaining a sample for laboratory analysis. This allows the choice of antibiotic therapy directed at the causative microorganisms.


Subject(s)
Humans , Female , Adult , Silicones , Breast Implants , Mastitis , Staphylococcus aureus , Wounds and Injuries , Breast Feeding , Diagnostic Imaging , Ultrasonography, Mammary , Infections , Inflammation , Anti-Bacterial Agents/therapeutic use
3.
Pesqui. vet. bras ; 41: e06129, 2021. tab
Article in English | LILACS, VETINDEX | ID: biblio-1180876

ABSTRACT

Mastitis occupies a prominent place among the diseases that affect dairy herds due to economic problems and public health. Staphylococcus spp. are infectious agents more involved in the etiology of caprine mastites, especially coagulase-negative Staphylococcus. Nineteen isolates of Staphylococcus spp. were obtained from subclinical caprine mastitis. All isolates were characterized by MALDI-TOF MS, being 47.36% (9/19) identified for S. epidermidis, 15.78% (3/19) for S. warneri, 10.52% (2/19) for S. aureus and S. caprae and 5.26% (1/19) for S. lugdunensis, S. simulans, and S. cohnii. All isolates characterized by MALDI-TOF were subjected a to polymerase chain reaction (PCR) for the 16S rRNA gene of Staphylococcus spp. to confirm the gender. After determining the species, tests for phenotypic detection of resistance to beta-lactams were carried out simple disk diffusion oxacillin, cefoxitin, penicillin G and amoxicillin + clavulanic acid, agar "screen" oxacillin and microdilution (MIC) cefoxitin. The disk diffusion test showed a strength of 58% (11/19) for penicillin G, 26.31% (5/19) for cefoxitin and 26.31% (5/19) for oxacillin. All strains were susceptible to amoxicillin + clavulanic acid and agar "screen" oxacillin. In the MIC, 63.15% (12/19) of the samples were cefoxitin resistant (MIC >4.0μg/ml). Then isolates were subjected to detection of the mecA resistance genes and regulators (mecl and mecRI), mecC and blaZ. Two samples of Staphylococcus epidermidis had the mecA gene. All isolates were negative for the mecA gene variant, mecl, mecRI, mecC and blaZ. These findings reinforce the importance of this group of microorganisms in the etiology of subclinical mastitis in goats and open perspectives for future research to investigate the epidemiology of the disease.(AU)


A mastite ocupa lugar de destaque entre as doenças que acometem o rebanho leiteiro, em virtude de problemas econômicos e de saúde pública. Staphylococcus spp. são os agentes infecciosos mais envolvidos na etiologia das mastites caprinas, principalmente Staphylococcus coagulase negativo. Dezenove isolados de Staphylococcus spp. foram obtidos a partir de mastite caprina subclínica. Todos os isolados foram caracterizados por MALDI-TOF MS, sendo 47,36% (9/19) identificadas como S. epidermidis, 15,78%(3/19) como S. warneri, 10,52% (2/19) como S. caprae e S. aureus e 5,26% (1/19) tanto para S. lugdunensis, como para S. simulans e S. cohnii. Todos os isolados caracterizados pelo MALDI-TOF foram submetidos a reação em cadeia da polimerase (PCR) para o gene 16rRNA de Staphylococcus spp. para a confirmação do gênero. Após a determinação da espécie, foram realizadas as provas para a detecção fenotípica de resistência aos beta-lactâmicos: difusão em disco simples de oxacilina, cefoxitina, penicilina G e amoxacilina +ácido clavulânico, ágar "screen" de oxacilina e microdiluição em caldo (MIC) de cefoxitina. O teste de difusão em disco demonstrou resistência de 58% (11/19) para penicilina G, 26,31% (5/19) para cefoxitina e 26,31% (5/19) para oxacilina. Todas as amostras foram sensíveis a amoxicilina + ácido clavulânico e ao ágar "screen" de oxacilina. Pelo MIC, 63,15% (12/19) das amostras foram resistentes a cefoxitina (MIC >4,0μg/ml). Em seguida os isolados foram submetidos a detecção dos genes de resistência mecA e seus reguladores (mecl e mecRI), mecC e blaZ. Duas amostras de S. epidermidis apresentaram o gene mecA. Todos os isolados foram negativos para a variante do gene mecA, mecl, mecRI, mecC e blaZ. Tais achados reforçam a importância deste grupo de microrganismos na etiologia da mastite subclínica em caprinos e abre perspectivas para futuras pesquisas para a investigação da epidemiologia da doença.(AU)


Subject(s)
Animals , Penicillin G , Staphylococcus , Ruminants , Goats , Proteomics , beta-Lactams , Mastitis , Polymerase Chain Reaction
4.
Pesqui. vet. bras ; 41: e06862, 2021. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1346693

ABSTRACT

Mastitis causes significant economic losses to the dairy cattle industry. The present study aimed to evaluate the antibacterial properties of 39 heterocyclic derivatives (1,3-thiazoles and 4-thiazolidinones) against clinical mastitis isolates from dairy cows. Milk samples were collected from cows with clinical mastitis and the bacterial species were identified by PCR. Antibacterial activity was assessed using the broth microdilution method. First, 39 heterocyclic compounds were tested against four bacterial isolates (Staphylococcus aureus, Streptococcus agalactiae, Corynebacterium bovis and Escherichia coli) randomly chosen from those recovered from the milk samples (Study 1). Subsequently, the compounds with the strongest antibacterial activity were tested against all the bacterial isolates recovered from the milk samples (Study 2). 1,3-thiazoles showed the strongest antibacterial activity, specially compounds 30 and 38, which also showed bactericidal properties according to their minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values. Corynebacterium spp. and Enterobacteriaceae isolates were the most susceptible to compounds 30 and 38. Compounds 30 and 38 are promising targets for new antimicrobial agents.(AU)


A mastite causa significativas perdas econômicas à indústria leiteira bovina. O presente estudo teve como objetivo avaliar as propriedades antibacterianas de 39 derivados heterocíclicos (1,3-tiazóis e 4-tiazolidinonas) contra isolados clínicos de mastite em vacas leiteiras. Amostras de leite foram coletadas de vacas com mastite clínica e as espécies bacterianas isoladas foram identificadas por PCR. A atividade antibacteriana foi avaliada pelo método de microdiluição em caldo. Primeiramente, os 39 compostos heterocíclicos foram testados contra quatro isolados bacterianos (Staphylococcus aureus, Streptococcus agalactiae, Corynebacterium bovis e Escherichia coli) escolhidos aleatoriamente dentre os recuperados das amostras de leite (Estudo 1). Posteriormente, compostos com atividade antibacteriana mais forte foram testados contra todos os isolados bacterianos recuperados das amostras de leite (Estudo 2). Os compostos 1,3-tiazóis apresentaram a maior atividade antibacteriana, principalmente os compostos 30 e 38, que também apresentaram propriedades bactericidas de acordo com seus valores de concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM). Os isolados Corynebacterium spp. e Enterobacteriaceae foram os mais suscetíveis aos compostos 30 e 38. Os compostos 30 e 38 mostraram-se promissores como novos agentes antimicrobianos.(AU)


Subject(s)
Animals , Cattle , Thiazoles/administration & dosage , Mastitis/immunology , Anti-Bacterial Agents , Staphylococcus aureus , Streptococcus agalactiae , Cattle/microbiology
5.
Autops. Case Rep ; 11: e2021252, 2021. graf
Article in English | LILACS | ID: biblio-1285405

ABSTRACT

Lactating adenoma is a rare benign breast lesion that most often presents as a small (up to 3 cm), solid, well-circumscribed, solitary, painless, mobile, lobulated mass. The highest incidence occurs in primiparous women (20 to 40 years old) during the third trimester of pregnancy. However, in the rare case presented herein, in addition to its giant size (more than 10 centimeters on palpation), this lactating adenoma is distinctive due to the presence of multiple nodules, poorly defined ultrasonographic margins, worrisome radiologic features, growth since early pregnancy, presence of infarction and association with chronic mastitis. From the clinical-radiologic perspective, the differential diagnoses included abscess associated with puerperal mastitis, phyllodes tumor, and galactocele. Biopsy was performed, and pathologic examination revealed the classic characteristics of lactating adenoma with multiple infarcted areas, leading to an unexpected confirmed case of giant lactating adenoma.


Subject(s)
Humans , Male , Pregnancy , Adult , Breast Neoplasms/pathology , Adenoma/pathology , Lactation Disorders/pathology , Diagnosis, Differential , Mastitis
6.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 13: 451-459, jan.-dez. 2021. ilus
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1151553

ABSTRACT

Objetivo: Avaliar as evidências, de produções científicas brasileiras, acerca da influência dos 10 passos para o sucesso do aleitamento materno na continuidade da amamentação. Método: revisão integrativa de produções científicas brasileiras, desenvolvida em junho de 2017, nas bases eletrônicas de dados LILACS, SCOPUS e PUBMED. Resultados: a normatização da assistência interfere positivamente na continuidade da amamentação. Treinar a equipe de saúde aumenta a prevalência de aleitamento materno. Orientar as vantagens e o manejo da lactação protege contra mastites, aleitamento misto e desmame precoce. Aqueles que receberam apoio no aleitamento materno na primeira meia hora após o parto estiveram mais propensos a mantê-lo no domicílio. Oferta de substitutos do leite materno, chupetas ou bicos artificiais interfere negativamente no estabelecimento e manutenção da amamentação. Conclusão: conclui-se, que os dez passos influenciam na continuidade da amamentação e, portanto, é importante que se amplie a cobertura nacional da IHAC


Objective: To evaluate the evidence of Brazilian scientific production about the influence of the 10 steps to succeed on breastfeeding continuity. Method: integrative review of Brazilian scientific literature developed at the databases LILACS, SCOPUS and MEDLINE in June of 2017. Results: the standardization of care positively interferes on breastfeeding continuity. Health team training increases the prevalence of breastfeeding. Professional orientation on the advantages and management of lactation protects against mastitis, mixed suckling and early weaning. Those who received support in breastfeeding within the first half hour after delivery were more likely to keep it at home. Provision of breast milk substitutes, pacifiers or artificial nipples interferes negatively in stablishing and maintaining breastfeeding. Conclusion: it is concluded that the ten steps influence on the continuity of breastfeeding and, therefore, it is important to expand the Child Friendly Hospital Initiative national coverage


Objetivo: Evaluar la evidencia de la producción científica brasileña sobre la influencia de los 10 pasos para tener éxito en la continuidad de la lactancia materna. Método: revisión integral de la literatura científica brasileña desarrollada en las bases de datos LILACS, SCOPUS y MEDLINE en junio de 2017. Resultados: La estandarización del cuidado interfiere positivamente en la continuidad de la lactancia materna. La capacitación de los equipos de salud aumenta la prevalencia de la lactancia materna. La orientación profesional sobre las ventajas y el manejo de la lactancia protege contra la mastitis, la lactancia mixta y el destete precoz. Aquellos que recibieron apoyo en la lactancia durante la primera media hora después del parto tenían más probabilidades de mantenerlo en casa. La provisión de sustitutos de la leche materna, chupones o pezones artificiales interfiere negativamente en el establecimiento y mantenimiento de la lactancia materna. Conclusión: se concluye que los diez pasos influyen en la continuidad de la lactancia materna y, por tanto, es importante ampliar la cobertura nacional de la Iniciativa Hospital Amigo de los Niños


Subject(s)
Humans , Female , Pregnancy , Infant, Newborn , Infant , Patient Care Team , Breast Feeding/instrumentation , Breast Feeding/methods , Health Education/methods , Weaning , Pacifiers , Parturition , Health Policy , Breast-Milk Substitutes , Mastitis
7.
Pesqui. vet. bras ; 40(12): 947-954, Dec. 2020. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1155047

ABSTRACT

Mastitis is a multifactorial disease and considered one of the most critical problems in the dairy industry worldwide. The condition is characterized by reduced milk and several abnormalities in the mammary gland. This study aimed to report an outbreak of gangrenous mastitis caused by multidrug-resistant Staphylococcus haemolyticus in a Santa Inês sheep herd. Eighteen sheep were affected, and five of them with severe clinical pictures were examined. The clinical and pathological picture were variable and characterized by apathy, anorexia, emaciation, opaque and brittle hair, apparent and congested episcleral vessels, and hyperthermia. These ewes had enlarged, firm, and painful mammary glands. Macroscopically, these lesions consisted of severe gangrenous mastitis, and microscopically, the primary lesions consisted of necrosis, thrombosis, and fibrosis of the mammary parenchyma. Milk samples from one of the five severely affected ewes were collected and cultured under aerobic or microaerophilic incubation at 37°C for 24 hours on sheep blood agar. The obtained colonies were then submitted to MALDI-TOF for speciation. The colonies were also submitted to an antimicrobial susceptibility test, genotyping of virulence factors and resistance genes were also performed. The isolates showed antimicrobial multiresistance since they were resistant to seven out of 13 tested antibiotics. The isolates were also positive for two staphylococcal enterotoxigenic genes (sec and see) and fibronectin-binding protein B (fnbB).(AU)


A mastite é uma doença multifatorial e é considerada um dos problemas mais importantes na indústria de laticínios no mundo todo. A condição é caracterizada pela redução de leite e várias anormalidades na glândula mamária. O objetivo deste estudo foi relatar um surto de mastite gangrenosa causada por Staphylococcus haemolyticus multirresistente em um rebanho ovino Santa Inês. Dezoito ovelhas foram afetadas e cinco delas com quadro clínico severo foram examinadas. O quadro clínico-patológico era variável quanto a severidade e consistia em apatia, anorexia, magreza, pelos opacos e quebradiços e vasos episclerais aparentes e ingurgitados. As ovelhas apresentavam glândulas aumentadas, firmes e dolorosas. Macroscopicamente, as principais lesões consistiam em mastite gangrenosa e microscopicamente havia necrose do parênquima glandular, trombose e fibrose. Amostras de leite de uma das cinco ovelhas severamente afetadas foram coletadas e cultivadas sob incubação aeróbica ou microaerofílica a 37°C por 24 horas em ágar sangue de ovelha. As colônias obtidas foram então submetidas ao MALDI-TOF para especiação. Além disso, as colônias foram submetidas a um teste de suscetibilidade antimicrobiana e foi realizada a genotipagem de fatores de virulência e genes de resistência. Os isolados apresentaram multirresistência antimicrobiana por serem resistentes a sete dos 13 antibióticos testados. Os isolados também foram positivos para dois genes enterotoxigênicos estafilocócicos (sec e see) e proteína B de ligação à fibronectina (fnbB).(AU)


Subject(s)
Animals , Female , Wounds and Injuries , Sheep/microbiology , Staphylococcus haemolyticus/pathogenicity , Mastitis/pathology , Disease Susceptibility
8.
Med. U.P.B ; 39(2): 41-48, 21/10/2020. Ilus
Article in Spanish | LILACS, COLNAL | ID: biblio-1123579

ABSTRACT

La mastitis granulomatosa es una enfermedad rara, de etiología desconocida y de diagnóstico de exclusión luego de descartar otras causas de mastitis, como las asociadas a infecciones bacterianas y a gérmenes atípicos como hongos y tuberculosis. La incidencia ha sido difícil de determinar, pero varia del 0.3% al 1.8%. Más frecuente en mujeres en edad reproductiva, la presentación clínica más común de este tipo de mastitis es una masa mamaria de consistencia dura, unilateral, asociada a dolor, eritema, calor e hinchazón, sin fiebre. Tiende a formar abscesos únicos o múltiples con fistulización, lo que hace que se confunda con carcinoma inflamatorio o localmente avanzado. Además, tiende a ser recurrente. El diagnóstico solo puede confirmarse por histopatología, en la que se evidencian granulomas no caseificantes concentrados en lobulillos mamarios, con infiltrado linfocitario crónico, necrosis y, con frecuencia, ectasia ductal. El tratamiento es controvertido, con opciones que van desde la observación, antibióticoterapia, terapia con corticosteroides, medicamentos inmunosupresores como el metotrexato, a la cirugía con escisión local amplia.


Granulomatous mastitis is a rare disease of unknown etiology, and the diagnosis is of exclusion after ruling out other causes of mastitis such as those associated with bacterial infectious diseases and by atypical germs such as fungi and tuberculosis. the incidence has been difficult to determine but varies from 0.3 to 1.8%. More common in women of reproductive age, the most common clinical presentation is a breast mass of hard, unilateral consistency, associated with pain, erythema, heat, swelling, and without fever. It tends to form single or multiple abscesses with fistulization, which makes it possible to be confused with an inflammatory or locally advanced carcinoma. Also with tendency to recurrence. The diagnosis can only be confirmed by histopathology, it is characterized by non-caseifying granulomas concentrated in breast lobules with chronic lymphocyte infiltrate, necrosis and often ductal ectasia. The treatment is controversial, with options ranging from observation, antibiotic therapy, corticosteroid therapy, immunosuppressive medications such as methotrexate, to surgery such as wide local excision.


A mastite granulomatosa é uma doença rara, de etiologia desconhecida e de diagnóstico de exclusão logo de descartar outras causas de mastite, como as associadas a infecções bacterianas e a germens atípicos como fungos e tuberculose. A incidência há sido difícil de determinar, mas varia de 0.3% a 1.8%. Mais frequente em mulheres em idade reprodutiva, a apresentação clínica mais comum deste tipo de mastite é uma massa mamária de consistência dura, unilateral, associada a dor, eritema, calor e inchaço, sem febre. Tende a formar abscessos únicos ou múltiplos com fistulização, o que faz confundir com carcinoma inflamatório ou localmente avançado. Além disso, tende a ser recorrente. O diagnóstico só se pode confirmar por histopatologia, na qual se evidenciam granulomas não caseificantes concentrados em lóbulos mamários, com infiltrado linfocitário crônico, necrose e, com frequência, extasia ductal. O tratamento é controvertido, com opções que vão desde a observação, antibióticoterapia, terapia com corticosteroides, medicamentos imunossupressores como o metotrexato, à cirurgia com incisão local ampla.


Subject(s)
Humans , Female , Granulomatous Mastitis , Women , Breast , Diagnosis , Abscess , Fever , Mastitis , Anti-Bacterial Agents
9.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 1047-1050, May-June, 2020. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1129749

ABSTRACT

The aim of the present study was to evaluate the antibacterial behavior of polypyrrole nanoparticles (PPy-NPs) in water against biofilm producer or not S. aureus isolated from cows and goats with mastitis. One hundred and thirty-eight isolates of S. aureus were initially evaluated for biofilm formation by spectrophotometry in microplates. In addition, the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of PPy-NPs in water for planktonic S. aureus were determined. From the bovine samples analyzed, 5 (4.46%) S. aureus isolates showed a strong biofilm production, 17 (15.18%) moderate production, 36 (32.14%) with weak production and 54 (48.21%) did not produce biofilms. Strains from goats (26) showed no biofilm production in 18 (69.23%) strains and weak biofilm production in 8 (30.76%) strains. The MIC and MBC of S. aureus to PPy-NPs were found in the same concentration (125搭/mL) in all strains tested, regardless of biofilm production or not. This finding provides a new insight into the interaction between PPy-NPs and S. aureus, and will offer potential benefits for the control of mastitis.(AU)


Subject(s)
Animals , Female , Cattle , Pyrroles/administration & dosage , Staphylococcus aureus/drug effects , Goats/microbiology , Mastitis/veterinary , Biofilms , Anti-Bacterial Agents/therapeutic use
10.
Arq. Inst. Biol ; 87: e0032020, 2020. tab
Article in English | LILACS, VETINDEX | ID: biblio-1130088

ABSTRACT

In order to characterize the milk production chain and study the dairy herd health in the city of Codó, state of Maranhão, Brazil, a checklist was applied and tests were carried out to detect clinical and subclinical mastitis using mastitis test strip cup and the California mastitis test (CMT), from June to August 2019, in 295 dairy cows from 20 farms. Water and milk samples were collected for microbiological analysis. It was observed that herdsmen do not have adequate knowledge about good agricultural practices. As for milking, only 60% are performed in corrals with coverage, and as for the floor, 60% are made of clay and 40% are made of concrete. In 35% of the properties, the water used in milking management comes from wells and the rest from dams. Pre and postdipping practices, CMT, mastitis strip cup test and the adoption of a mastitis control program are not carried out on any of the properties Two cows tested positive for subclinical mastitis and one cow tested positive for tuberculosis. In the microbiological analysis of the milk, a high count of total coliforms and thermotolerants was obtained, with values between 23 to > 1,100 MPN/mL and < 3.0 to > 1,100 MPN/mL, respectively. The presence of coagulase positive staphylococci was also observed in 25% of the samples. The water samples also showed high contamination by total coliforms between 4.1 to > 2.419.6 MPN/mL and 40% showed the presence of Escherichia coli. These results reflect the need for more investments in technical assistance and technical training for these producers.(AU)


Este estudo teve como objetivo caracterizar a cadeia produtiva do leite e estudar a sanidade do rebanho leiteiro do município de Codó, estado do Maranhão, Brasil, através da aplicação de um checklist e da realização de exames para detecção de mastite clínica e subclínica pelos métodos da caneca do fundo escuro e California mastitis test (CMT), no período de junho a agosto de 2019, em 295 vacas leiteiras procedentes de 20 propriedades. Coletaram-se amostras de leite e água da ordenha para análises microbiológicas. Observou-se que os ordenhadores não possuem conhecimento adequado sobre as boas práticas agropecuárias. Quanto à realização da ordenha, apenas 60% realizam-na em currais com cobertura; quanto ao piso, 60% são de terra batida e 40% de concreto. A água utilizada no manejo da ordenha é proveniente de poços em 35% das propriedades e as demais de açudes. As práticas de pré e pós-dipping e os testes CMT e caneca de fundo escuro e a adoção de programa de controle da mastite não são realizados em nenhuma das propriedades. Diagnosticaram-se duas vacas com mastite subclínica e uma com tuberculose e verificou-se elevada contaminação por coliformes totais e termotolerantes nas análises microbiológicas do leite, variando entre 23 NMP/mL a >1.100 NMP/mL e < 3.0 a > 1.100 NMP/mL, respectivamente, e presença de estafilococos coagulase positivos em 25% das amostras. As amostras de água também apresentaram elevada contaminação por coliformes totais entre 4,1 NMP/mL a > 2.419,6 NMP/mL e 40% apresentaram presença de Escherichia coli. Esses resultados refletem a necessidade de mais investimentos em assistência técnica e treinamento técnico para esses produtores.(AU)


Subject(s)
Animals , Cattle , Milk/microbiology , Livestock , Food Safety , Microbiological Techniques , Coagulase , Food Quality Standards , Escherichia coli , Multiple Tube Method , Checklist , Mastitis
11.
Pesqui. vet. bras ; 39(12): 954-960, Dec. 2019. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1056928

ABSTRACT

The indiscriminate use of antibiotics in the treatment of caprine mastitis causes the appearance of resistant microorganisms, besides leaving residues in milk, putting at risk to human health. In this way, propolis is an alternative in the treatment of diseases because it has antimicrobial activity, mainly because of the presence of flavonoids in its composition. The aim of this study was to evaluate the antimicrobial potential of propolis to Staphylococcus spp. Isolated from cases of goat mastitis and qualify the crude ethanoic extract by high performance liquid chromatography (HPLC). In this study, the minimum bactericidal concentration values of propolis extracts in ethanol, ethyl acetate and hexane showed that the best concentrations capable of promoting the highest mortality of the isolates of Staphylococcus spp. from mastitis in goats, were 6250, 3125 and 1562.5µg/mL, respectively. By the microplate adherence test, it was found that 20.78% isolates were not able to form biofilm, 14.70% were classified as moderate and 64.70% were weak and none as a strong biofilm producer. Propolis in its different diluents was able to affect the formation of biofilm and showed a pronounced marked antimicrobial activity against Staphylococcus spp. strains and may be indicated for use in in vivo studies.(AU)


O uso indiscriminado de antibióticos no tratamento de mastite caprina leva ao desenvolvimento de micro-organismos resistentes que poderão estar presentes em alimentos, colocando em risco a saúde humana. Dessa forma, a própolis surge como uma alternativa para o tratamento de doenças por possuir uma ação antimicrobiana, principalmente pela presença de flavonoides em sua composição. O objetivo desse estudo foi avaliar o potencial antimicrobiano da própolis frente à Staphylococcus spp. isolados de casos de mastite caprina e qualificar o extrato etanoico bruto por cromatografia líquida de alta eficiência (CLAE-DAD). Neste estudo, os valores de concentração bactericida mínima (CBM) dos extratos de própolis em álcool etílico, acetato de etila e hexano nos isolados foram de 6250, 3125 e 1562,5µg/mL, respectivamente. Pelo teste de aderência à microplacas, observou-se que 20,78% dos microorganismos, não foram capazes de formar biofilme, 14,70% foram classificados como moderados, 64,70% em fracos e nenhum como forte produtor de biofilme. A própolis em seus diferentes diluentes foi capaz de afetar a formação de biofilme e apresentou significativa atividade antimicrobiana frente a cepas de Staphylococcus spp., podendo ser indicada para utilização em estudos "in vivo".(AU)


Subject(s)
Animals , Female , Propolis/therapeutic use , Staphylococcal Infections/therapy , Staphylococcal Infections/veterinary , Staphylococcus/isolation & purification , Goats/microbiology , Apitherapy/veterinary , Mastitis/therapy , Mastitis/veterinary
12.
Med. U.P.B ; 38(2): 140-146, 17 de octubre de 2019.
Article in Spanish | LILACS | ID: biblio-1023409

ABSTRACT

La mastitis puerperal es un problema de salud importante en el posparto, la incidencia varía del 2% al 33%. Se define como la inflamación de los lóbulos glandulares, siendo la infección la etiología más frecuente y el puerperio, en las primeras semanas, el periodo de mayor prevalencia. La mayoría de las mujeres experimentan algún grado de congestión mamaria, al preparar la glándula para la eyección láctea durante el amamantamiento, pero cuando la técnica es inadecuada y lleva a fisuras del pezón, se genera riesgo de colonización bacteriana de los ductos mamarios con sobreinfección e, incluso, formación de abscesos, que van a requerir manejo hospitalario, antibioticoterapia e intervenciones quirúrgicas. Es esta la causa más importante de suspensión de la lactancia y su repercusión en la alimentación y desarrollo del recién nacido. No hay uniformidad en los criterios y el diagnóstico sigue siendo fundamentalmente clínico. El microrganismo más implicado es el estafilococo, su confirmación microbiológica se hace a partir del Gram y cultivo del drenaje de abscesos o de cultivos de leche, siendo estos, pocas veces realizados, a menos que el cuadro se complique con repercusión sistémica (sepsis) o no haya respuesta a la antibioticoterapia empírica. El tratamiento fundamental es el drenaje del seno, por lo que debe mantenerse la lactancia.


Puerperal mastitis is a major health problem in the postpartum period, whose incidence varies from 2% to 33%. It is defined as the inflammation of the glandular lobes. The infection would be the most frequent etiology, and the puerperium would be the period of highest prevalence in the first weeks. In general, most women experience some degree of breast engorgement, preparing the mammary gland for milk ejection during breastfeeding, but when the technique is inadequate and leads to nipple fissures, there is a risk of bacterial colonization of the mammary ducts with superinfection and even the formation of abscesses, which will require hospital management, antibiotic therapy and surgical interventions. This is the most important cause of breastfeeding suspension and its impact on the feeding and development of the newborn. There is no uniformity in the diagnostic criteria. It remains fundamentally clinical. The most implicated microorganism is staphylococcus. Its microbiological confirmation is made from the Gram and the culture of the drainage of abscesses, or milk cultures. These are only a few times carried out, unless the patient's record gets complicated with systemic repercussion (sepsis) or there is no response to empirical antibiotic therapy. The fundamental treatment is sinus drainage; therefore, breastfeeding should be maintained.


A mastite puerperal é um problema de saúde importante no pós-parto, a incidência varia de 2% a 33%. Se define como a inflamação dos lóbulos glandulares, sendo a infecção a etiologia mais frequente e o puerpério, nas primeiras semanas, o período de maior prevalência. A maioria das mulheres experimentam algum grau de congestão mamária, ao preparar a glândula para a ejeção láctea durante a amamentação, mas quando a técnica é inadequada e leva a fissuras do mamilo, se gera risco de colonização bacteriana dos ductos mamários com sobreinfecção e, incluso, formação de abscessos, que vão a requerer manejo hospitalar, antibioticoterapia e intervenções cirúrgicas. É esta a causa mais importante de suspensão da amamentação e sua repercussão na alimentação e desenvolvimento do recém-nascido. Não há uniformidade nos critérios e o diagnóstico segue sendo fundamentalmente clínico. O microrganismo mais implicado é o estafilococo, sua confirmação microbiológica se faz a partir do Gram e cultivo da drenagem de abscessos ou de cultivos de leite, sendo estes, poucas vezes realizados, a menos que o quadro se complique com repercussão sistémica (sepse) ou não haja resposta à antibioticoterapia empírica. O tratamento fundamental é a drenagem do seio, pelo que deve manter-se a amamentação.


Subject(s)
Breast , Abscess , Mastitis
13.
Rev. cuba. pediatr ; 91(3): e616, jul.-set. 2019. tab
Article in Spanish | LILACS, CUMED | ID: biblio-1093716

ABSTRACT

Introducción: Las infecciones de piel y partes blandas constituyen una de las causas más frecuentes de consulta pediátrica, de lo cual no está exento el recién nacido, que por sus peculiaridades anatómicas, fisiológicas e inmunológicas, pueden convertirse en afecciones letales. Objetivo: Describir aspectos clínicos y epidemiológicos de los recién nacidos con infecciones de piel y partes blandas. Métodos: Se realizó un estudio observacional, prospectivo y transversal en 256 pacientes que ingresaron en el Departamento de Neonatología del Hospital Pediátrico Universitario William Soler de enero de 2013 a diciembre de 2015. Se determinó la incidencia de las infecciones de piel y partes blandas, formas clínicas de presentación, edad, sexo, estadía, terapéutica utilizada y microorganismos aislados en hemocultivo. Resultados: En la muestra, 95,3 por ciento de los neonatos tenían más de 7 días de nacidos y 59,0 por ciento eran hembras. La tasa de incidencia fue de 16,1 × 100 ingresos y el 75,0 por ciento presentaba mastitis. El microorganismo más aislado en hemocultivos fue Staphylococcus aureus. El 60,9 por ciento de los pacientes recibieron monoterapia con Cefazolina. Conclusiones: La incidencia de las infecciones de piel y partes blandas fue mayor en el año 2014; los recién nacidos inician la enfermedad en la segunda semana de vida, en el sexo femenino, con una estadía hospitalaria menor de una semana. El microorganismo más aislado en hemocultivos es Staphylococcus aureus. Más de la mitad de los pacientes reciben monoterapia con Cefalosporina de primera generación como terapéutica con una evolución favorable(AU)


Introduction: Skin and soft tissue infections constitute one of the most frequent causes of pediatric consultations. Newborns are not exempt of that since their anatomic, physiological and immunological peculiarities can help to become those infections in letal ones. Objective: To describe clinical and epidemiological aspects of newborns presenting skin and soft tissue infections. Methods: An observational, prospective and cross-sectional study was carried out in 256 patients admitted in the Neonatology Department of William Soler Pediatric University Hospital from January, 2013 to Decmeber, 2015. There were determined: the incidence of skin and soft tissue infections, the clinical kinds, age, sex, stay, used therapy, microorganisms isolated in hemocultures. Results: In the sample, 95.3 percent of the newborns were of more than 7 days alive and 59.0 percent were female. The incidence rate was of 16.1 × 100 admissions, and 75.0 percent presented mastitis. The most isolated in hemocultures microorganism was Staphylococcus aureus. 60.9 percent of the patients had monotherapy with Cefazolin. Conclusions: The incidence of skin and soft tissue infections was higher in 2014; the disease onset in newborns in the second week of life, in females, with a hospital stay of less than a week. The most isolated in hemocultures microorganism is Staphylococcus aureus. More tan half of the patients undergone monotherapy with first generation Cephalosporines as a therapy with a favorable evolution(AU)


Subject(s)
Humans , Male , Female , Infant, Newborn , Skin Diseases, Infectious/complications , Infant, Newborn, Diseases/prevention & control , Cross-Sectional Studies , Prospective Studies , Observational Study , Mastitis/prevention & control
14.
Rev. argent. mastología ; 38(138): 45-54, jul 2019. ilus
Article in Spanish | LILACS | ID: biblio-1116809

ABSTRACT

Introducción La mastitis granulomatosa es unaenfermedad infrecuente caracterizada por una inflamación granulomatosa crónica de los lobulillos mamarios. Las opciones de tratamiento siguen siendo controvertidas. Objetivo El objetivo del presente estudio fue analizar nuestra experiencia en el diagnóstico y tratamiento de las pacientes con mastitis granulomatosas, valorando la conveniencia de tratamientos quirúrgicos o tratamientos más conservadores. Material y método Se presenta un estudio retrospectivo y descriptivo de las pacientes diagnosticadas y tratadas en nuestro centro desde enero de 2010 hasta diciembre de 2018. Se analizaron las características clínicas y radiológicas de cada una, así como el tratamiento y su evolución. Resultados Los resultados del estudio fueron los siguientes: • El número de pacientes fue 10; el tiempo medio de seguimiento fue de 10,5 meses (rango 2-49 meses); la mediana de edad fue de 44,5 años (rango 31-81 años);ocho pacientes (80%)se manifestaron como tumoración palpable; el tiempo medio de duración de los síntomas fue de 6,8 meses (rango 2-24 meses); el tamaño medio de la lesión alcanzó los 23,6 mm (rango 12-40); una paciente se clasificó como bi-rads 2, 1 paciente como bi-rads 3, 1 paciente como bi-rads 4y 3 pacientes como bi-rads 5. • El tratamiento fue quirúrgico en 6 ocasiones (4 resecciones y 2 drenajes con biopsia) y médico en 4 ocasiones. Siete de las pacientes (70%) se curaron con el tratamiento efectuado (5 con cirugía y 2 con tratamiento conservador). Tres pacientes presentaron recurrencia o persistencia (1 con cirugía y 2 con tratamiento conservador). Conclusiones La mastitis granulomatosa es una enfermedad infrecuente y de causa desconocida, con tendencia a la recurrencia y cronicidad, cuyo tratamiento es todavía motivo de controversia


Introduction Granulomatous mastitis is an infrequent disease characterized by a chronic granulomatous inflammation of mammary lobules. Treatment options remain controversial. Objective The aim of the present study was to analyze our experience in the diagnosis and treatment of patients with granulomatous mastitis, assessing the convenience of surgical treatments or more conservative treatments. Materials and method This is a retrospective and descriptive study of the patients diagnosed and treated in our center from January 2010 to December 2018. We analyzed the clinical and radiological characteristics of each one, as well as the treatment and its evolution. Results • Number of patients 10; mean time of follow-up 10.5 months (range 2-49 months); median of age 44.5 years (range 31-81 years); eight patients (80%) manifested as a palpable tumor;mean duration of symptoms was 6.8 months (range 2-24 months); mean lesion size of 23.6mm (range 12-40); one patient was classified as bi-rads 2, 1 patient as bi-rads 3, 1 patient as bi-rads 4 and 3 patients as bi-rads 5. • The treatment was surgical 6 times (4 resections and 2 drainages with biopsy) and doctor on 4 occasions. Seven of the patients (70%) were cured with the treatment performed (5 with surgery and 2 with conservative treatment). Three patients presented recurrence or persistence (1 with surgery and 2 with conservative treatment). Conclusions Granulomatous mastitis is an infrequent disease of unknown cause, with a tendency to recurrence and chronicity, whose treatment is still controversial


Subject(s)
General Surgery , Granulomatous Mastitis , Mastitis
15.
Article in English | WPRIM | ID: wpr-758948

ABSTRACT

As an important zoonotic pathogen, Staphylococcus aureus has led to serious mastitis and endometritis in infected dairy cows. In this study, a total of 164 strains of S. aureus were isolated from dairy cows in Xinjiang Province, China, and subjected to assays to determine drug susceptibility and biofilm (BF) formation ability. Enterotoxin-related genes were detected, and the transcription levels of genes related to BF formation were determined by using reverse transcription-quantitative polymerase chain reaction. Moreover, the pathogenicity of isolates with different BF formation abilities was determined by measuring their hemolysis activity, half lethal dose (LD₅₀) and organ bacterial load. The results showed that 86.0% of S. aureus isolates could form BF. Among them, 42.1% of the strains had weak BF formation ability, and most strains with a strong BF formation ability were ica gene carriers. The S. aureus isolates displayed multidrug resistance and their drug resistance was positively correlated with their BF formation ability. Moreover, 96.3% of the S. aureus isolates carried enterotoxin genes. Among them, the detection rates of the novel enterotoxin genes were higher than those of conventional enterotoxin genes. Furthermore, isolates with a strong BF formation ability had higher LD50 but lower hemolysis ability and organ bacterial load than those of the isolates with weak or no BF ability. However, isolates without BF ability produced more severe pathological changes than those of isolates with strong BF formation ability. These findings suggest that higher BF ability and presence of novel enterotoxin genes are important characteristics of S. aureus isolates from dairy cows in Xinjiang Province, China, and such isolates may pose potential threats to food safety.


Subject(s)
Bacterial Load , Biofilms , China , Drug Resistance , Drug Resistance, Microbial , Drug Resistance, Multiple , Endometritis , Enterotoxins , Female , Food Safety , Hemolysis , Lethal Dose 50 , Mastitis , Polymerase Chain Reaction , Staphylococcus aureus , Staphylococcus , Virulence
16.
Article in English | WPRIM | ID: wpr-758910

ABSTRACT

Buffalo mastitis is an important economic problem in southern Italy, causing qualitative/quantitative alterations in milk and resulting in economic losses due to the sub-clinical course and chronic evolution. We investigated 50 udders of slaughtered buffaloes and subjected them to effectual microbiological screening to evaluate macro and microscopic mammary gland changes, immune-characterize the cell infiltrates, and compare the degree of tissue inflammation with somatic cell counts. Numerous Gram-positive and Gram-negative bacteria were isolated from all samples, majority of which were environmental mastitis pathogens. Histological features referable to chronic mastitis were observed in 92% udders. Lymphocytes, plasma cells and macrophages were found to evolve into aggregates in 48% udders, which often organized to form tertiary lymphoid structures (TLSs). A predominance of interstitial CD8+ over CD4+ lymphocytes and, in TLSs, scattered CD8+ lymphocytes in the mantle cells and CD79+ lymphocytes in germinal centers, were evidenced. Environmental pathogens are known to persist and cause chronic inflammatory changes in buffaloes, where CD8+ lymphocytes play an important role by controlling the local immune response. Moreover, the TLSs evidenced here for the first time in buffalo mastitis, could play a role in maintaining immune responses against persistent antigens, thereby contributing in determining the chronic course of mastitis.


Subject(s)
Animals , Buffaloes , Cell Count , Female , Germinal Center , Gram-Negative Bacteria , Inflammation , Italy , Lymphocytes , Macrophages , Mammary Glands, Animal , Mammary Glands, Human , Mass Screening , Mastitis , Milk , Plasma Cells
17.
Article in English | WPRIM | ID: wpr-758896

ABSTRACT

Mammary lesions in sows can prevent suckling piglets from consuming colostrum that provides fundamental nutrients and protective immunity. Although mammary gross lesions are frequently found in sows at farms or slaughterhouses, with the exception of mastitis, they have received little research attention. In this study, we investigated mammary lesions observed in South Korean sows between 2015 and 2016. Mammary tissue samples of 82 sows showing gross lesions during meat inspection were histologically classified and immunohistochemical analysis was conducted to assess the expression of estrogen receptor (ER)-α, ER-β, and progesterone receptor (PR) for mammary hyperplastic lesions as well as that of cluster of differentiation (CD) 3, CD79a, interleukin (IL)-1α, IL-1β, IL-6, and IL-8 for mastitis. Furthermore, 20 swab samples were cultured, and the isolated bacteria were identified using polymerase chain reactions for 16S ribosomal RNA genes. The lesions were classified as hyperplasia, mastitis, or hyperplasia with mastitis. Immunohistochemistry results revealed that there was neither expression of ER-α nor of ER-β, but all examined hyperplastic samples expressed PR. In addition, there was a significant correlation between CD3 and IL-1β expressions, as well as between IL-1β and IL-6 expressions. Regarding the identity of the isolated bacteria, Pseudomonas spp. were most frequently detected. The results of this study have revealed the incidence and characteristics of porcine mammary lesions.


Subject(s)
Abattoirs , Agriculture , Bacteria , Bacterial Infections , Classification , Colostrum , Cytokines , Estrogens , Female , Hyperplasia , Immunohistochemistry , Incidence , Interleukin-6 , Interleukin-8 , Interleukins , Mammary Glands, Human , Mastitis , Meat , Polymerase Chain Reaction , Pseudomonas , Receptors, Progesterone , RNA, Ribosomal, 16S , Swine
18.
Article in English | WPRIM | ID: wpr-761720

ABSTRACT

Trypanosomiasis is caused by a pathogenic protozoan of the genus Trypanosoma, being Trypanosoma vivax the most important agent for cattle. The aim of the present study was to demonstrate the expansion of T. vivax infection in different mesoregions of Minas Gerais, Brazil, and describe the clinicopathological findings of trypanosomiasis in cattle. The diagnosis was based on visualization of the parasite in blood smears and DNA detection of T. vivax in the blood of live cows and tissues of necropsied animals by the polymerase chain reaction (PCR). Thirty suspected herds were tested, of which 11 were positive for T. vivax. The most frequent clinical signs were anemia, apathy, drop in milk production, weight loss, reproductive disorders, and nervous signs. Concomitant diseases, such as malignant edema, pneumonia and increased cases of mastitis were associated with T. vivax infection. Three cows were necropsied and the most significant findings were low body condition score, pale mucous and spleen with white pulp hyperplasia. The results demonstrated the expansion of T. vivax infection in Minas Gerais, that PCR-associated blood smears are promising for diagnosis, and that other diseases often occur concomitantly to T. vivax infection in regions with trypanosomiasis in cattle.


Subject(s)
Anemia , Animals , Apathy , Brazil , Cattle , Diagnosis , DNA , Edema , Female , Hyperplasia , Mastitis , Milk , Parasites , Parasitic Diseases , Pneumonia , Polymerase Chain Reaction , Ruminants , Spleen , Trypanosoma vivax , Trypanosoma , Trypanosomiasis , Weight Loss
19.
Rev. cuba. pediatr ; 90(4): e621, set.-dic. 2018. graf
Article in Spanish | LILACS, CUMED | ID: biblio-978471

ABSTRACT

Introducción: La parotiditis supurativa aguda se presenta con poca frecuencia en el período neonatal. Objetivo: Aportar un nuevo caso de parotiditis supurativa aguda por Staphylococcus aureus resistente a la Meticillina. Presentación del caso: El presente caso tiene la particularidad de que presentó foco de infección inicial (impétigo y conjuntamente mastitis bilateral), en el que se demostró el mismo microorganismo causal de la infección: Staphylococcus aureus resistente a la Meticillina. La mastitis bilateral evolucionó hacia la formación de absceso. En la literatura revisada solo se encontraron tres publicaciones que tratan de neonatos con un foco inicial de infección en sitios diferentes de la parotiditis. Estos aspectos fueron los que motivaron la presentación del caso. Conclusiones: Staphylococcus aureus resistente a la Meticillina ha emergido en los últimos años como agente causal de parotiditis supurativa aguda, que puede diseminarse hacia otro foco infeccioso, habitualmente se logra la curación con tratamiento antibiótico ajustado al agente causal, concretamente con Vancomicina, aunque puede requerir también tratamiento quirúrgico si ocurre abscedación(AU)


Introduction: Acute suppurative parotitis occurs infrequently in the neonatal period. Objective: To provide information of a new case of acute suppurative parotitis caused by Methicillin- resistant Staphylococcus aureus. Case presentation: The present case has the particularity that the patient presented a source of initial infection (impetigo and jointly bilateral mastitis), in which the same causal microorganism of the infection was found: Methicillin resistant Staphylococcus aureus. Bilateral mastitis evolved to the formation of abscess. In the literature reviewed, there were only 3 publications on neonates who presented an initial source of infection in sites different from parotitis. These aspects are those that motivated the presentation of this case. Conclusions: Methicillin resistant Staphylococcus aureus has emerged in the last years as a causal agent of acute suppurative parotitis that can lead to dissemination of another source of infection. Normally, the cure is achieved with antibiotic treatment adjusted to the causal microorganism, specifically with Vancomycin; although it can require surgical treatment if abscesses occurs(AU)


Subject(s)
Humans , Female , Infant, Newborn , Parotitis/complications , Parotitis/drug therapy , Vancomycin/therapeutic use , Methicillin-Resistant Staphylococcus aureus/drug effects , Staphylococcal Infections/complications , Case Reports , Mastitis/complications , Mastitis/drug therapy
20.
Pesqui. vet. bras ; 38(12): 2194-2200, dez. 2018. tab
Article in English | LILACS, VETINDEX | ID: biblio-976427

ABSTRACT

Mastitis represents an important health problem for Santa Inês breed, causing losses to the producer, due to loss of ewes or the decrease in weight gain of lambs. The aim of this work was to assess the health of the mammary gland of Santa Inês ewes at the drying and puerperium and to investigate the efficacy of a dry-off therapy with gentamicin. In this study, 64 ewes were divided in a control group (GC) and treatment group (GT), and the health of the mammary gland was assessed at the drying and puerperium. The GT ewes received 250mg of gentamicin (Gentocin® DryCow/Schering-Plough, product indicated for use in dairy cows) in each mammary half. For diagnosis, clinical examination, California Mastitis Test, somatic cell count and milk culture was performed. In the GC, of the 45 (70.3%) healthy mammary halves at the drying, 12 developed subclinical mastitis and nine clinical mastitis at the puerperium. In the GT, among 51 (79.7%) healthy mammary halves at the drying, six developed subclinical mastitis and 11 clinical mastitis at the puerperium. No association was observed between treatment and the occurrence of mastitis at puerperium. Of the 19 (29.7%) mammary halves of the GC that presented subclinical mastitis at the drying, three remained with subclinical mastitis and five developed clinical mastitis at the puerperium. In the GT, of the 13 (20.3%) mammary halves that had subclinical mastitis at the drying, four remained with subclinical mastitis and four developed clinical mastitis. No association was observed between treatment and cure or persistence of mastitis at the puerperium. The main microorganisms isolated, at the drying and puerperium, from animals with subclinical or clinical mastitis were Staphylococcus spp., predominantly coagulase negative Staphylococcus (CSN). At the puerperium, 29 cases of clinical mastitis occurred, 19 with isolation, where 10 were CNS and six S. aureus. Mannheimia haemolytica was isolated in one case of subclinical mastitis and other of clinical mastitis. News protocols and different ways of handling at drying and at puerperium must be investigated.(AU)


A mastite é um problema sanitário importante em ovelhas da raça Santa Inês, ocasionando prejuízo ao produtor em virtude do descarte de matrizes e da queda no ganho de peso dos cordeiros. O objetivo deste trabalho foi avaliar a saúde da glândula mamária de ovelhas da raça Santa Inês na secagem e no puerpério e pesquisar a eficácia da terapia intramamária com gentamicina na secagem. Sessenta e quatro ovelhas foram divididas em grupos controle (GC) e tratamento (GT), cada um contendo 32 animais, e a saúde da glândula mamária avaliada na secagem e no puerpério. As ovelhas do GT receberam 250mg de gentamicina (Gentocin® Mastite Vaca Seca/ Schering-Plough Veterinária, produto indicado pela empresa para utilização em vacas de leite) em cada metade mamária. Para o diagnóstico, foram realizados exame físico da glândula mamária, California Mastitis Test, contagem de células somáticas e cultura do leite. No GC, das 45 (70,3%) metades mamárias sadias na secagem, 12 desenvolveram mastite subclínica e nove mastite clínica no puerpério. No GT, das 51 (79,7%) metades mamárias sadias na secagem, seis desenvolveram mastite subclínica e 11 mastite clínica no puerpério. Não houve associação entre o tratamento e a ocorrência de mastite no puerpério. Das 19 (29,7%) metades mamárias do GC que apresentaram mastite subclínica na secagem, três permaneceram com mastite subclínica e cinco desenvolveram mastite clínica no puerpério. No GT, das 13 (20,3%) metades mamárias com mastite subclínica na secagem, quatro permaneceram com mastite subclínica e quatro desenvolveram mastite clínica. Não houve associação entre o tratamento e a cura ou persistência da mastite no puerpério. Os principais micro-organismos isolados, na secagem e puerpério, de animais com mastite subclínica ou clínica foram Staphylococcus spp., com predominância de Staphylococcus Coagulase Negativa (SCN). No puerpério, ocorreram 29 casos de mastite clínica, sendo 19 com isolamento, 10 com SCN e seis com S. aureus. Mannheimia haemolytica foi isolado em um caso de mastite subclínica e um caso de mastite clínica. Novos protocolos e diferentes formas de manejo na secagem e no puerpério devem ser pesquisados.(AU)


Subject(s)
Animals , Female , Lactation , Gentamicins/therapeutic use , Sheep, Domestic/injuries , Postpartum Period , Mastitis/veterinary , Staphylococcus/pathogenicity
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