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1.
Arq. Inst. Biol ; 88: e00212020, 2021.
Article in English | LILACS, VETINDEX | ID: biblio-1349023

ABSTRACT

The consumption of meat and meat products can pose consumers into risk due to the presence of biological hazards that can cause foodborne diseases. Thus, this study aimed to compare the microbiological quality of illegal and inspected salami sold in the state of São Paulo, Brazil. For this purpose, 80 salami samples (40 illegal and 40 inspected) were purchased and their microbiological quality was assessed according to the protocol established by the Brazilian Ministry of Health. All samples were considered as acceptable for consumption according to the Brazilian law. However, the samples of illegal salami were significantly higher contaminated with bacteria belonging to the genus Staphylococcus (p = 0.002) and had a higher trend to be contaminated with total coliforms (p = 0.08) and thermotolerant ones (p = 0.07) compared to inspected salami. Salmonella spp. and coagulase-positive Staphylococcus were not detected. In conclusion, although all samples were considered as safe for consumption, illegal salami had a worse microbiological quality when compared to inspected ones.


Subject(s)
Humans , Animals , Staphylococcus/pathogenicity , Meat/microbiology , Meat Products , Salmonella , Bacteria , Health Surveillance , Food Quality , Public Health , Commerce , Food Safety , Foodborne Diseases
2.
Braz. j. biol ; 81(2): 424-436, 2021. tab, ilus
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1153346

ABSTRACT

Pathogenic Yersinia enterocolitica (Y. enterocolitica) is one of the food-borne entero-pathogen responsible for yersiniosis in humans. The purpose of this research was to survey the prevalence, virulence-associated genes, and antimicrobial resistance of Y. enterocolitica isolated from meat and meat product samples in Egypt. Forty-one (5.9%) out of 700- samples of chicken meat, beef, ground beef, and sausage were positive Y. enterocolitica with a high prevalence in chicken meat (12%). Five virulence genes (ail, inv, ystA, ystB, and yadA) were characterized among 41 Y. enterocolitica isolates with variable frequencies. Among the strains tested, the ystB gene was detected with a high percentage (78.1%), followed by inv gene (70.7%), ail gene (14.6%), ystA gene (12.2%), and yadA gene (2.4%). A high resistance rate was estimated to amoxicillin-clavulanic acid (100%), followed by cefazolin (95%), ampicillin (65.9%), and doxycycline (51.2%), whilst a high sensitivity rate was observed to gentamicin and ciprofloxacin (97.6% each). Interestingly, the multidrug resistance was specified in the 70.7% of strains and showing 13 resistance patterns. Based on nucleotide sequence analysis of the 16s rRNA gene, the phylogenetic tree showed the genetic relatedness amongst Y. enterocolitica isolates. These findings highlighted the emergence of virulent and multidrug-resistant pathogenic Y. entrocolitica in retailed meat and meat products in Egypt.


A Yersinia enterocolitica patogênica (Y. enterocolitica) é um dos enteropatógenos de origem alimentar responsáveis pela yersiniose no ser humano. O objetivo desta pesquisa foi avaliar a prevalência, genes associados à virulência e resistência antimicrobiana de Y. enterocolitica isolada de amostras de carne e produtos à base de carne no Egito. Quarenta e um (5,9%) de 700 amostras de carne de frango, carne bovina, moída e linguiça foram Y. enterocolitica positivas, com alta prevalência em carne de frango (12%). Cinco genes de virulência (ail, inv, ystA, ystB e yadA) foram caracterizados entre 41 isolados de Y. enterocolitica com frequências variáveis. Entre as cepas testadas, o gene ystB foi detectado com uma alta porcentagem (78,1%), seguido pelo gene inv (70,7%), ail genes (14,6%), gene ystA (12,2%) e gene yadA (2,4%). Foi estimada uma alta taxa de resistência ao ácido amoxicilina-clavulânico (100%), seguida de cefazolina (95%), ampicilina (65,9%) e doxiciclina (51,2%), enquanto uma alta taxa de sensibilidade foi observada para gentamicina e ciprofloxacina (97,6% cada). Curiosamente, a resistência a múltiplas drogas foi especificada em 70,7% das cepas e mostrando 13 padrões de resistência. Com base na análise da sequência nucleotídica do gene rRNA 16s, a árvore filogenética mostrou a relação genética entre isolados de Y. enterocolitica. Esses achados destacaram o surgimento de Y. entrocolitica patogênica virulenta e multirresistente em carnes e produtos à base de carne no Egito.


Subject(s)
Humans , Yersinia enterocolitica/genetics , Drug Resistance, Bacterial/genetics , Meat/microbiology , Meat Products/microbiology , Phylogeny , Virulence/genetics , RNA, Ribosomal, 16S , Egypt , Genotype , Anti-Bacterial Agents/pharmacology
3.
Braz. j. biol ; 79(3): 460-465, July-Sept. 2019. tab, graf
Article in English | LILACS | ID: biblio-1001467

ABSTRACT

Abstract The fidelity of the genomes is defended by mechanism known as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems. Three Type II CRISPR systems (CRISPR1- cas, CRISPR2 and CRISPR3-cas) have been identified in enterococci isolates from clinical and environmental samples. The aim of this study was to observe the distribution of CRISPR1-cas, CRISPR2 and CRISPR3-cas in non-clinical strains of Enterococcus faecalis and Enterococcus faecium isolates from food and fecal samples, including wild marine animals. The presence of CRISPRs was evaluated by PCR in 120 enterococci strains, 67 E. faecalis and 53 E. faecium. It is the first report of the presence of the CRISPRs system in E. faecalis and E. faecium strains isolated from wild marine animal fecal samples. The results showed that in non-clinical strains, the CRISPRs were more frequently detected in E. faecalis than in E. faecium. And the frequencies of CRISPR1-cas and CRISPR2 were higher (60%) in E. faecalis strains isolated from animal feces, compared to food samples. Both strains showed low frequencies of CRISPR3-cas (8.95% and 1.88%). In conclusion, the differences in the habitats of enterococcal species may be related with the results observe in distribution of CRISPRs systems.


Resumo A fidelidade dos genomas ​​é defendida por mecanismos conhecidos como sistemas de repetições palindrômicas curtas agrupadas e regularmente interespaçadas (CRISPRs). Três tipos de sistemas CRISPR II (CRISPR1-cas, CRISPR2 e CRISPR3-cas) têm sido identificados em cepas de enterococos isolados de amostras clínicas e ambientais. O objetivo deste estudo foi observar a distribuição dos CRISPR1-cas, CRISPR2 e CRISPR3-cas em cepas não-clínicas de Enterococcus faecalis e Enterococcus faecium isoladas de amostras alimentícias e fecais, incluindo animais marinhos selvagens. A presenca dos CRISPRs foi determinada por PCR em 120 cepas de enterococos, sendo 67 E. faecalis e 53 E. faecium. É o primeiro relato da presença do sistema CRISPRs nas estirpes E. faecalis e E. faecium isoladas de amostras fecais de animais marinhos selvagens. Os resultados mostraram que em cepas não-clínicas, os CRISPRs foram mais frequentemente detectados em E. faecalis do que em E. faecium. E as frequências de CRISPR1-cas e CRISPR2 foram maiores (60%) em cepas de E. faecalis isoladas de fezes de animais, quando comparadas à amostras de alimentos. Ambas as cepas apresentaram baixas freqüências de CRISPR3-cas (8,95% e 1,88%). Em conclusão, as diferenças nos habitats das espécies de enterococos podem estar relacionadas com os resultados observados na distribuição dos sistemas CRISPRs.


Subject(s)
Animals , Enterococcus faecium/genetics , Enterococcus faecalis/genetics , Feces/microbiology , Clustered Regularly Interspaced Short Palindromic Repeats , Food Microbiology , Turtles/microbiology , Vegetables/microbiology , Chickens/microbiology , Dairy Products/microbiology , Milk/microbiology , Spheniscidae/microbiology , Fur Seals/microbiology , Meat/microbiology
4.
Braz. J. Vet. Res. Anim. Sci. (Online) ; 56(2): [e150446], ago. 2019. tab
Article in English | LILACS, VETINDEX | ID: biblio-1025062

ABSTRACT

Poultry meat is often involved as a vehicle for microorganisms that cause food-borne diseases. Salmonella sp. is a major pathogen involved in outbreaks around the world. Based on its importance, the objective of this study was to determine the occurrence of Salmonella sp. in frozen and chilled poultry carcasses slaughtered and marketed in the western region of Paraná state, Brazil. A total of 340 samples were collected between January 2007 and April 2013, with 66 (19.41%) carcasses positive for Salmonella sp. It can be concluded from the results obtained that the occurrence of Salmonella sp. in poultry carcasses remains high, even though it is a product marketed refrigerated or frozen, methods considered appropriate for food preservation. A lower occurrence result was expected due to the 14-year implementation of the national pathogen reduction program established by the Ministry of Agriculture, Livestock and Supply aimed at a gradual reduction in the occurrence of Salmonella sp. in these products through constant monitoring of carcasses immediately after slaughter.(AU)


A carne de aves é um alimento que frequentemente encontra-se envolvido como veículo de micro-organismos causadores de enfermidades, sendo Salmonella sp. um desses agentes comumente envolvidos nessas enfermidades em todo o mundo. Com isso, o objetivo deste estudo foi determinar a ocorrência de Salmonella sp. em carcaças de frango congeladas e refrigeradas, abatidas e comercializadas na região oeste do estado do Paraná. Um total de 340 amostras foram coletadas entre janeiro de 2007 e abril de 2013. Das amostras analisadas, 66 (19,41%) das carcaças foram positivas para Salmonella sp. De acordo com os resultados obtidos pode-se concluir que a ocorrência de Salmonella sp. em carcaças de frangos permanece alta, mesmo sendo produtos comercializados refrigerados ou congelados, métodos considerados adequados para conservação de alimentos. Um resultado de menor ocorrência era esperado devido à implementação de 14 anos do Programa Nacional de Redução de Patógenos estabelecido pelo Ministério da Agricultura, Pecuária e Abastecimento visando uma redução gradual na ocorrência de Salmonella sp. nestes produtos através de monitoramento constante de carcaças imediatamente após o abate.(AU)


Subject(s)
Animals , Salmonella/classification , Meat/microbiology , Commerce/classification , Animal Culling
5.
Braz. j. microbiol ; 49(4): 936-941, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-1039269

ABSTRACT

ABSTRACT Shigatoxigenic and enteropathogenic Escherichia coli with virulence and multidrug resistance profile were isolated from Nile tilapia. This study finding is of great importance to public health because they help understand this pathogen epidemiology in fish and demonstrate how these animals can transmit E. coli related diseases to humans.


Subject(s)
Humans , Animals , Enteropathogenic Escherichia coli/isolation & purification , Shiga-Toxigenic Escherichia coli/isolation & purification , Fishes/microbiology , Phylogeny , Food Contamination/analysis , Consumer Product Safety , Escherichia coli Proteins/genetics , Enteropathogenic Escherichia coli/classification , Enteropathogenic Escherichia coli/genetics , Shiga-Toxigenic Escherichia coli/classification , Shiga-Toxigenic Escherichia coli/genetics , Meat/microbiology
6.
Braz. j. microbiol ; 49(3): 607-613, July-Sept. 2018. tab
Article in English | LILACS | ID: biblio-951802

ABSTRACT

Abstract The effect of different modified atmosphere packaging regimes on the behavior of Salmonella spp. on minced meat was studied. Minced meat was experimentally contaminated with a Salmonella spp. cocktail (S. Enteritidis, S. Typhimurium, S. Infantis and S. Arizonae), packaged under vacuum or modified atmosphere with initial headspaces containing 20%O2/50%CO2/30%N2 and 20%O2/30%CO2/50%N2) and stored at 3 ± 1 °C for 12 days. Samples were analyzed for Salmonella spp., viable and lactic acid bacteria count every third day. Salmonella spp. counts decreased during storage in all packaging types, with reductions of about 1.5 log CFU/g. A significant difference (p < 0.01) was noted between Salmonella spp. counts in meat packaged in vacuum and modified atmospheres, although there was no significant difference in Salmonella spp. count between meat packaged in 50%CO2, and meat packaged in 30%CO2. At the end of the study, there were significant differences (p < 0.01; p < 0.05) in total viable and lactic acid bacterial counts between meat packaged in vacuum and modified atmosphere, and the lowest counts were noted in meat packaged in modified atmosphere with 50%CO2.


Subject(s)
Animals , Cattle , Salmonella/growth & development , Food Packaging/methods , Microbial Viability , Meat/microbiology , Salmonella/isolation & purification , Salmonella/genetics , Swine , Vacuum , Colony Count, Microbial , Food Packaging/instrumentation , Meat/analysis
8.
Hig. aliment ; 32(282/283): 40-43, jul.-ago. 2018. ilus
Article in Portuguese | LILACS | ID: biblio-946665

ABSTRACT

O hambúrguer é um produto industrializado de origem animal, extremamente utilizado pela população principalmente pela sua praticidade no consumo. Esse trabalho teve como objetivo realizar a análise microbiológica de hambúrgueres congelados comercializados em Maceió-AL. Foram selecionadas nove marcas comerciais de hambúrgueres dos tipos: bovino, frango e misto (bovino e frango), foram pesquisados os seguintes micro-organismos: bactérias do grupo coliformes, Estafilococos coagulase positiva e Salmonella sp. As amostras das marcas comerciais B (misto), E (frango), F (misto), H (bovino) apresentaram contaminação por coliformes a 35 ºC e 45 ºC, porém dentro dos padrões da legislação brasileira. Para inibir o crescimento de micro-organismos nos alimentos cárneos é necessário manter a temperatura de armazenamento e distribuição a -18 °C, além da conservação destes em equipamentos adequadamente higienizados, de forma a garantir a qualidade sanitária do produto. De acordo com os resultados encontrados, todos os hambúrgueres congelados industrializados avaliados estavam aptos para consumo.


The burger is a product of animal origin used by the population, highly industrialized mainly for your convenience in consumption. This work aimed to carry out microbiological analysis of frozen hamburgers marketed in enniskillen has been selected nine trademarks of burgers: beef, chicken and mixed (beef and chicken), were searched the following microorganisms: bacteria of the coliform group, coagulase positive and salmonella sp. In trademarks b (mixed), and(chicken), f (mixed), h (veal) samples obtained coliform contamination to 35° c and 45° c, but within the brazilian legislation standards. To inhibit the growth of microorganisms in meat food is necessary to maintain the temperature of storage and distribution, in-18° c, in addition to the conservation of these properly sanitized equipment, to ensure the sanitary quality of the product. According to the results all the frozen processed burgers were able for consumption.


Subject(s)
Animals , Cattle , Food Contamination/analysis , Colimetry/methods , Meat/microbiology , Salmonella/pathogenicity , Prospective Studies , Industrialized Foods , Food Storage/standards , Food Preservation/standards , Frozen Foods/microbiology
9.
Braz. j. microbiol ; 49(2): 320-328, Apr.-June 2018. tab, graf
Article in English | LILACS | ID: biblio-889239

ABSTRACT

Abstract Detection of Salmonella is very important to minimize the food safety risk. In this study, the recombinant PagC protein and PagC antibody were prepared and coupled with immunomagnetic beads (IMBs) to capture Salmonella cells from pork and milk samples. And then the SYBR Green qualitative PCR was developed to detect the pathogenic Salmonella. The results showed that the PagC polyclonal antiserum is of good specificity and the capture rate of 0.1 mg IMBs for Salmonella tended to be stable at the range of 70-74% corresponding to the concentrations between 101 and 104 CFU/mL. The method developed demonstrated high specificity for the positive Salmonella samples when compared to non-specific DNA samples, such as Escherichia coli, Staphylococcus aureus, Yersinia enterocolitica, and Yersinia pseudotuberculosis. The limit of detection of this assay was 18 CFU/mL. Detection and quantitative enumeration of Salmonella in samples of pork or milk shows good recoveries of 54.34% and 52.07%. In conclusion, the polyclonal antibody of recombinant PagC protein is effective to capture Salmonella from detected samples. The developed pagC antibody IMBs-qPCR method showed efficiency, sensitivity and specificity for 30 Salmonella detection, enabling detection within 10 h, which is a promising rapid method to detect Salmonella in emergency.


Subject(s)
Animals , Salmonella/isolation & purification , Food Contamination , Immunomagnetic Separation/methods , Real-Time Polymerase Chain Reaction/methods , Food Microbiology/methods , Salmonella/genetics , Bacterial Proteins/immunology , Sensitivity and Specificity , Milk/microbiology , Meat/microbiology , Antibodies, Bacterial/immunology , Antibodies, Bacterial/metabolism
11.
Hig. aliment ; 32(276/277): 103-107, fev. 27, 2018.
Article in Portuguese | LILACS | ID: biblio-884013

ABSTRACT

[{"text": "A campilobacteriose é uma zoonose\r\nemergente de origem alimentar\r\ncausada por bactérias do gênero\r\nCampylobacter. Vários fatores dificultam\r\no isolamento deste patógeno\r\nem amostras naturalmente contaminadas,\r\npor isso devem ser utilizadas\r\nmetodologias normalizadas bem\r\ncomo meios de cultura com desempenho\r\nadequado, prevenindo a ocorrência\r\nde resultados falso negativos.\r\nAssim, avaliou-se a performance\r\nde meios de cultura recomendados\r\npelas ISO 10272-1 para detecção\r\nde Campylobacterspp. com testes\r\nde seletividade e produtividade em\r\nculturas puras e o desempenho destes\r\nmeios em amostras de carne de\r\nfrango artificialmente contaminadas.\r\nCepas ATCC de C. coli e C. jejuni e\r\ndos interferentes S. aureus, E. coli e\r\nProteusmirabilis foram inoculadas\r\nnos meios indicados pelas normas\r\noficiais e posteriormente inoculados\r\nem amostras fortificadas. Os meios\r\ntestados, tanto em culturas puras\r\nquanto em amostras fortificadas,\r\ntiveram desempenho satisfatório,\r\nmostrando boa seletividade e produtividade,\r\npermitindo que os laboratórios\r\noptem pela combinação de\r\nmeios com melhor performance para\r\nisolamento e identificação de Campylobacter\r\nspp. em amostras naturalmente\r\ncontaminadas.(AU)", "_i": "pt"}]


Subject(s)
Animals , Campylobacter/isolation & purification , Campylobacter Infections , Food Contamination/analysis , Culture Media , Food Microbiology , Meat/microbiology , Poultry/microbiology , Food Samples
12.
Arq. bras. med. vet. zootec. (Online) ; 70(2): 623-627, mar.-abr. 2018. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-910971

ABSTRACT

In order to evaluate the microbiological quality of broiler chickens produced in Minas Gerais State, 240 samples of broiler carcasses from the five regions of the Minas Gerais State were collected, by official inspection services, for one year. The samples were submitted to counts of total and thermotolerant coliforms, coagulase-positive and negative Staphylococcus, besides Campylobacter spp., Listeria monocytogenes, E.coli O157:H7, and Salmonella spp. resource. The results showed the presence of total and thermotolerant coliforms in 34.2% and 13.5% of broiler carcasses evaluated, respectively. All tested samples were positive for Staphylococcus spp., 9.1% for Salmonella spp., 15.5% for Listeria monocytogenes, and 2.1% for Campylobacter spp. E.coli O157:H7 was not isolated from the samples.(AU)


Subject(s)
Animals , Meat/classification , Meat/microbiology , Campylobacter , Listeria monocytogenes , Poultry , Staphylococcus
13.
Braz. j. microbiol ; 49(supl.1): 113-118, 2018. tab, graf
Article in English | LILACS | ID: biblio-974319

ABSTRACT

Abstract Poultry meat is a food product that usually carries high rates of microbial contamination, including foodborne pathogens. The poultry industry has established different systems to minimize these hazards. In recent years, extensive literature has demonstrated the antimicrobial activity of different contact surfaces made of copper to effectively reduce microbial loads. The aim of the present study was to evaluate the antibacterial effect of copper surfaces on the transmission of two foodborne pathogens - Salmonella enterica and Listeria monocytogenes - and a poultry native microbiota bacterial species - Enterobacter cloacae. We also evaluated the impact of the poultry meat matrix on the antimicrobial activity of a copper surface. Our results indicated that copper surfaces reduced the bacterial load quickly (<than 4 min) when the microorganisms were exposed to polished copper surfaces. Even when bacteria were inoculated on copper surfaces soiled with the organic matrix (washing water from poultry carcasses) and survival rates were significantly higher, an antimicrobial effect was still observed. Survival rates of two microorganisms simultaneously exposed to copper did not show significant differences. We found an antimicrobial effect over pathogenic and non-pathogenic microorganisms. Results suggest a potential role for copper surfaces in the control of microbiological hazards in the poultry industry.


Subject(s)
Animals , Poultry/microbiology , Copper/pharmacology , Meat/microbiology , Anti-Bacterial Agents/pharmacology , Cadaver , Food Contamination/analysis , Salmonella enterica/drug effects , Microbiota/drug effects , Listeria monocytogenes/drug effects
14.
Pesqui. vet. bras ; 37(12): 1373-1379, dez. 2017. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-895409

ABSTRACT

O objetivo deste trabalho foi introduzir a técnica de espectrometria de massa com fonte de ionização e dessorção a laser assistida por matriz e analisador de tempo-de-voo (MALDI-TOF) para incrementar o método tradicional microbiológico na detecção de Salmonella spp. e Escherichia coli em carcaças bovinas. Foram avaliadas 270 amostras de 90 carcaças de bovinos. Para isolamento de Salmonella spp. e E. coli, foram utilizadas, respectivamente, as metodologias descritas na ISO 6579:2002 e no Compendium of Methods for the Microbiological Examination of Foods. As análises por MALDI-TOF foram realizadas a partir de isolados cultivados em ágar nutriente ou em caldo triptona de soja, provenientes das amostras com características bioquímicas positivas (n=7), inconclusivas (n=4) e negativas (n=85) para Salmonella spp. e bioquímicas positivas (n=37) e negativas (n=85) para E. coli. Os perfis de massas foram adquiridos com o espectrômetro de massas MALDI-TOF Autoflex III SmartBeam e os espectros brutos foram processados usando o programa MALDI Biotyper (Bruker Daltonics). De acordo com a identificação preliminar, com base na morfologia das colônias e nas reações bioquímicas, sete isolados foram considerados positivos para Salmonella spp. Através do MALDI Biotyper, esses sete isolados foram classificados como pertencentes ao gênero Salmonella e, além disso, identificados como S. enterica. Quatro isolados que apresentaram características fenotípicas não usuais e resultados inconclusivos nos testes bioquímicos para Salmonella foram identificados como pertencentes aos gêneros Citrobacter e Proteus após análise por MALDI. Para E. coli, 37 amostras foram positivas pelos testes bioquímicos da espécie, o que foi confirmado por MALDI Biotyper. A metodologia MALDI-TOF permitiu a rápida confirmação da identidade de Salmonella spp. e E. coli, podendo ser utilizada para detecção desses microrganismos em isolados bacterianos de carcaças bovinas.(AU)


The aim of this study was to introduce matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry to improve the traditional microbiological method for the detection of Salmonella spp. and Escherichia coli in beef carcasses. Two hundred seventy samples from 90 beef carcasses were evaluated. The methodologies described in ISO 6579:2002 and in the Compendium of Methods for the Microbiological Examination of Foods were used for Salmonella spp. and E. coli isolation, respectively. MALDI-TOF analysis were performed on tryptone soya broth suspension isolates or directly from nutrient agar colonies, from the positive, inconclusive or negative biochemically tested samples for Salmonella and E. coli. Mass profiles were acquired on an Autoflex III SmartBeam MALDI-TOF mass spectrometer and the raw spectra were processed using the MALDI Biotyper software (Bruker Daltonics). According to the preliminary identification based on colony morphology and the biochemical reactions, seven isolates were positive for Salmonella spp. Through MALDI Biotyper these seven isolates were also classified as belonging to the genus Salmonella and further identified as S. enterica. Four isolates showing unusual phenotypic characteristics and inconclusive results in biochemical tests for Salmonella were identified as belonging to Citrobacter and Proteus genera after MALDI analysis. Regarding Escherichia coli, 37 were positive for species biochemical testing which MALDI Biotyper confirmed. MALDI-TOF methodology allowed rapid Salmonella spp. and E. coli identity confirmation and may be used to detect these microrganisms within bacterial isolates from beef carcasses.(AU)


Subject(s)
Animals , Cattle , Salmonella , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/veterinary , Escherichia coli , Meat/microbiology , Mass Spectrometry/veterinary , Abattoirs , Enterobacteriaceae
15.
Braz. j. microbiol ; 48(4): 689-694, Oct.-Dec. 2017. tab, graf
Article in English | LILACS | ID: biblio-889163

ABSTRACT

ABSTRACT The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment. L. monocytogenes was isolated from 11.2% of samples. The highest prevalence was observed in frozen foods (38%), followed by cheese (10%). 1/2b and 4b were the most frequently identified serotypes. In six of 236 analyzed establishments we successively recovered L. monocytogenes isolates belonging to the same serotype. Most of them corresponded to serotype 1/2b. Pulsed-field gel electrophoresis profiles suggest that at least 33% of L. monocytogenes 1/2b isolates are genetically related and that may remain viable for prolonged periods. The observed prevalence of L. monocytogenes was lower than reported in neighboring countries. Our findings highlight the role that frozen foods may play in the spread of this pathogen, and the relevance of serotypes 1/2b and 4b.


Subject(s)
Animals , Cheese/microbiology , Fast Foods/microbiology , Frozen Foods/microbiology , Listeria monocytogenes/isolation & purification , Meat/microbiology , Food Contamination/analysis , Food Contamination/statistics & numerical data , Food Microbiology , Listeria monocytogenes/classification , Listeria monocytogenes/genetics , Prevalence , Serogroup , Uruguay
16.
Hig. aliment ; 31(272/273): 80-83, 30/10/2017.
Article in Portuguese | LILACS | ID: biblio-876166

ABSTRACT

O trabalho realizado teve como objetivo avaliar a qualidade higienicossanitária de carcaças bovinas, oriundas de abatedouros-frigoríficos sob Inspeção Distrital (Dipova) e Federal (SIF), localizados na região do Distrito Federal e Entorno. Foram realizadas análises microbiológicas para Contagem de micro-organismos Mesófilos aeróbios estritos e facultativos, identificação do Número mais Provável de Coliformes a 45°C e Contagem de Staphylococcus aureus em 05 (cinco) diferentes pontos de 07 (sete) meias carcaças, nas quais as coletas foram realizadas antes e depois da lavagem com água clorada, perfazendo assim um total de 70 análises. Os valores médios dos resultados observados, antes da lavagem com água clorada, para Contagem de micro-organismos Mesófilos aeróbios estritos e facultativos, foram de 9,7 UFC/cm²; para Número Mais provável de Coliformes a 45ºC, de 29 germes/cm² e para Staphylococcus coagulase positivo, de 1 UFC/cm². Os valores observados nas meias carcaças, após a lavagem com água clorada na Contagem de micro- -organismos Mesófilos aeróbios estritos e facultativos foram de 1,5 UFC/ cm²; para a análise de Número Mais Provável de Coliformes a 45ºC, foi de 8,2 germes/cm² e não houve crescimento de Staphylococcus coagulase positiva. Neste trabalho verificou-se a presença de Coliformes a 45ºC e a Contagem de micro-organismos Mesófilos aeróbios estritos e facultativos antes e após a lavagem das meias carcaças e a presença de Staphylococcus coagulase positiva apenas antes das lavagem das mesmas. A lavagem das meias carcaças com água clorada diminuiu o grau de contaminação.(AU)


Subject(s)
Animals , Cattle , Food Inspection , Meat/analysis , Meat/microbiology , Slaughterhouse Sanitation , Chlorine , Disinfection/methods , Food Contamination/prevention & control , Food Safety
17.
Hig. aliment ; 31(272/273): 84-89, 30/10/2017.
Article in Portuguese | LILACS | ID: biblio-876168

ABSTRACT

A pesquisa de micro-organismos nos alimentos é de vital importância, uma vez que, além de causadores de toxinfecções alimentares, servem de barreira às exportações e diminuem o tempo de vida de prateleira de produtos alimentícios. Garantir a inocuidade dos alimentos, assim como monitorar a contaminação dos produtos, é crucial para a saúde pública. Para garantir um alimento com qualidade e inocuidade é necessário uniformizar toda a cadeia produtiva desde a obtenção da matéria-prima até o produto final. No presente estudo, pesquisou-se a presença de Salmonella em amostras de linfonodos mesentéricos e conteúdo intestinal, bem como em quatro pontos da carcaça (papada, barriga, lombo e pernil), em 25 suínos abatidos em abatedouro- frigorífico sob inspeção municipal, provenientes de unidades produtivas que utilizam sobras alimentares como ração dos suínos. Verificou-se a presença de S. Saintpaul em conteúdo intestinal (4%) e linfonodos mesentéricos (12%), perfazendo um total de quatro (16%) suínos portadores; bem como a presenças de S. Saintpaul e S. Enteritidis em suabes de papada (8,0%). Em 18 isolados de Salmonella observou-se resistência a tetraciclina (66,6%), sulfa + trimetropim (38,8%), cloranfenicol (27,7%), nitrofurantoína (22,2%), cefalotina (22,2%), ampicilina (0,1%) e ácido nalidíxico (0,05%). A presença de suínos portadores de salmonela indica a necessidade de medidas preventivas no processo produtivo destes animais. Entretanto, a presença do agente na carcaça é indicativa de falhas na aplicação das boas práticas durante o abate.(AU)


Subject(s)
Animals , Food Contamination/analysis , Food Inspection , Food Microbiology , Meat/microbiology , Salmonella/isolation & purification , Abattoirs/standards , Anti-Bacterial Agents/therapeutic use , Drug Resistance, Bacterial , Swine
18.
Hig. aliment ; 31(270/271): 124-129, 29/08/2017.
Article in Portuguese | LILACS | ID: biblio-848956

ABSTRACT

A carne bovina in natura constitui um comércio importante e bastante procurado pela população, entretanto está envolvida no aparecimento de inúmeras doenças transmitidas por alimentos. Objetivou-se no presente trabalho avaliar o perfil microbiológico, através da pesquisa de Coliformes totais, Coliformes termotolerantes, Salmonella spp. e Staphylococcus aureus, em amostras de carne bovina in natura comercializada no Município de Picos, Piauí. Coletaram-se 30 amostras de carne (patinho bovino) obtidas em mercados públicos e supermercados, em seguida as amostras foram conduzidas em caixas isotérmicas para o Laboratório de Microbiologia de Alimentos da Universidade Federal do Piauí para análises microbiológicas. Em todas as amostras processadas foi detectada a presença de coliformes totais e Coliformes termotolerantes. Com relação à pesquisa de Salmonella spp, nos supermercados, duas amostras (33,3%) apresentaram Salmonella spp. Nos mercados públicos 14 amostras (58,33%) apresentaram contaminação com Salmonella spp. Com relação à presença de Staphylococcus aureus, 22 amostras (73,33%) apresentaram contaminação acima de 105 UFC/g. De acordo com os resultados obtidos, conclui- -se que as elevadas populações dos micro-organismos aqui pesquisados evidenciaram um produto com risco de ocasionar toxinfecções alimentares, necessitando a intervenção dos órgãos de Vigilância Sanitária para implantar medidas de melhorias nas condições de produção, manuseio e de venda deste produto.(AU)


Subject(s)
Animals , Cattle , Food Contamination/analysis , Food Microbiology , Meat/microbiology , Brazil , Coliforms , Food Samples , Staphylococcus aureus/isolation & purification
19.
Braz. j. microbiol ; 48(2): 352-358, April.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-839386

ABSTRACT

Abstract Meat is one of the most perishable foods owing to its nutrient availability, high water activity, and pH around 5.6. These properties are highly conducive for microbial growth. Fresh meat, when exposed to oxygen, is subjected to the action of aerobic psychrotrophic, proteolytic, and lipolytic spoilage microorganisms, such as Pseudomonas spp. The spoilage results in the appearance of slime and off-flavor in food. In order to predict the growth of Pseudomonas fluorescens in fresh meat at different pH values, stored under refrigeration, and temperature abuse, microbial mathematical modeling was applied. The primary Baranyi and Roberts and the modified Gompertz models were fitted to the experimental data to obtain the growth parameters. The Ratkowsky extended model was used to determine the effect of pH and temperature on the growth parameter µmax. The program DMFit 3.0 was used for model adjustment and fitting. The experimental data showed good fit for both the models tested, and the primary and secondary models based on the Baranyi and Roberts models showed better validation. Thus, these models can be applied to predict the growth of P. fluorescens under the conditions tested.


Subject(s)
Temperature , Pseudomonas fluorescens/growth & development , Food Microbiology/methods , Hydrogen-Ion Concentration , Models, Theoretical , Pseudomonas fluorescens/drug effects , Pseudomonas fluorescens/radiation effects , Aerobiosis , Meat/microbiology
20.
Hig. aliment ; 31(268/269): 102-105, 30/06/2017.
Article in Portuguese | LILACS | ID: biblio-846494

ABSTRACT

A larva do nematódeo Trichinella spiralis é responsável por infectar animais como porcos, javalis e cavalos e por causar grave doença em seres humanos. Surtos devido ao consumo da carne de cavalo são mais comuns na Europa, principalmente na França e Itália, onde existe a cultura de ingeri-la crua. Apesar de no Brasil não ter relatos sobre a infecção desse parasita em animais e seres humanos, os abatedouros exportadores para alguns mercados, como Europa e Estados Unidos, são obrigados a pesquisar o parasita. No presente estudo, a presença de larvas de Trichinella spiralis foi avaliada em 14.852 cavalos abatidos entre dezembro de 2014 a julho de 2016 em um frigorífico de Araguari sob inspeção federal. Os animais eram procedentes dos estados de Minas Gerais, Goiás e Bahia. A técnica empregada foi a de digestão artificial, em que se utilizou o músculo masseter, seguindo as legislações da União Europeia: Diretiva 91/497/CEE, Diretiva 77/96/CEE, Regulamento (CE) N° 2075/2005 e suas alterações. A larva não foi encontrada em nenhuma amostra analisada.


Subject(s)
Animals , Food Contamination/analysis , Food Microbiology , Meat/microbiology , Trichinella spiralis/parasitology , Brazil , Food Inspection , Horses , Trichinella spiralis/isolation & purification , Trichinellosis/diagnosis
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