ABSTRACT
To study the chemical constituents from the rhizome of Menispermum dauricum,fifteen compounds,N-methylcorydaldine( 1),thalifoline( 2),stepholidine( 3),acutumine( 4),daurisoline( 5),acutumidine( 6),dauricicoline( 7),bianfugecine( 8),6-O-demethylmenisporphine( 9),bianfugedine( 10),dauricoside( 11),eleutheroside D( 12),aristolactone( 13),aristoloterpenateⅠ( 14) and aristolochic acid( 15) were isolated from 75% ethanol extract of Menispermi Rhizoma by using thin layer chromatography and column chromatography methods. Their structures were identified based on their physicochemical properties and spectral data. Among them,compounds 12-15 were obtained from the genus Menispermum for the first time. Six alkaloids with higher contents were subjected to evaluate the anti-hypoxic activities by using MTT method. As a result,six alkaloids exhibited significant anti-hypoxia activities.
Subject(s)
Humans , Alkaloids , Hypoxia , Menispermum , Plant Extracts , RhizomeABSTRACT
OBJECTIVE@#To explore the anti-tumor effects of asiatic moonseed rhizome extraction-dauricine on bladder cancer EJ cell strain, prostate cancer PC-3Mcell strain and primary cell culture system.@*METHODS@#The main effective component-phenolic alkaloids ofMenispermum dauricum was extracted and separated from asiatic moonseed rhizome by chemical method. MTT method was used to detect dauricine anti-tumor effect.@*RESULTS@#Dauricine had an obvious proliferation inhibition effect on the main tumor cells in urinary system. The minimum drug sensitivity concentration was between 3.81-5.15 μg/mL, and the inhibition ratio increased with the increase of concentration.@*CONCLUSIONS@#Dauricine, the main effective component extracted from asiatic moonseed rhizome, had a good inhibition effect on tumor cells in urinary system. At the same time, Dauricine has certain inhibition effects on the primary cultured tumor cell.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacokinetics , Pharmacology , Benzylisoquinolines , Chemistry , Pharmacokinetics , Pharmacology , Cell Line, Tumor , Cell Proliferation , Menispermum , Chemistry , Plant Extracts , Chemistry , Pharmacology , Rhizome , Chemistry , Tetrahydroisoquinolines , Chemistry , Pharmacokinetics , Pharmacology , Urinary Bladder Neoplasms , Drug Therapy , PathologyABSTRACT
<p><b>AIM</b>To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on thrombosis and platelet aggregation, and to explore its mechanism of action.</p><p><b>METHODS</b>Thrombosis was observed with arteriovenous shunt thrombus model in rat; platelet aggregation was determined by Born's method; ultrastructure of platelet was observed by transmission electron microscope; TXB2 or 6-keto-PGF1alpha levels were assessed by radioimmunoassay; and NO was determined by colorimetric method.</p><p><b>RESULTS</b>PAMD dose-dependently inhibited experimental thrombus formation, platelet aggregation induced by ADP, AA and THR in vivo and ultrastructure changes stimulated by THR; PAMD increased the generation of 6-keto-PGF1alpha in thoracic aortae and NO level in plasma; and had no influence on TXB2 release (P > 0.05).</p><p><b>CONCLUSION</b>PAMD inhibited thrombosis and platelet aggregation, and its mechanism might be due to the increase of PGI2 and NO level.</p>
Subject(s)
Animals , Male , Rabbits , Rats , 6-Ketoprostaglandin F1 alpha , Metabolism , Alkaloids , Pharmacology , Aorta, Thoracic , Metabolism , Benzylisoquinolines , Pharmacology , Blood Platelets , Dose-Response Relationship, Drug , Epoprostenol , Metabolism , Menispermum , Chemistry , Nitric Oxide , Blood , Plants, Medicinal , Chemistry , Platelet Aggregation , Rats, Sprague-Dawley , Rhizome , Chemistry , Tetrahydroisoquinolines , Pharmacology , Thrombosis , Metabolism , Thromboxane B2 , MetabolismABSTRACT
<p><b>AIM</b>To study the protective effects of phenolic alkaloids from Menispermum dauricum (PAMd) on inflammatory injury following focal cerebral ischemia-reperfusion in rats.</p><p><b>METHODS</b>The right middle cerebral artery of the rat was occluded by inserting a nylon suture through the internal carotid artery for 2 h, followed by reperfusion by withdrawing the suture. The expression of intercellular adhesion molecule-1 (ICAM-1) was observed by immunohistochemistry staining. The adhesiveness and infiltration of leucocytes were observed by HE staining. The activity of myeloperoxidase (MPO) and the content of nitric oxide (NO) in the cortex and hippocampus were measured.</p><p><b>RESULTS</b>PAMd was shown to markedly inhibit ICAM-1 expression, alleviate the adhesiveness and infiltration of leucocytes, and decrease the MPO activity and the NO content in ischemic cortex and hippocampus.</p><p><b>CONCLUSION</b>PAMd has protective effects on inflammatory injury following focal cerebral ischemia-reperfusion by inhibiting ICAM-1 expression, alleviating the adhesiveness and infiltration of leucocytes and decreasing the generation of NO.</p>
Subject(s)
Animals , Female , Male , Rats , Alkaloids , Pharmacology , Anti-Inflammatory Agents, Non-Steroidal , Pharmacology , Benzylisoquinolines , Pharmacology , Brain Ischemia , Metabolism , Cell Adhesion , Cerebral Cortex , Metabolism , Hippocampus , Metabolism , Intercellular Adhesion Molecule-1 , Metabolism , Leukocytes , Pathology , Menispermum , Chemistry , Neuroprotective Agents , Pharmacology , Neutrophil Infiltration , Nitric Oxide , Metabolism , Peroxidase , Metabolism , Plants, Medicinal , Chemistry , Rats, Wistar , Reperfusion Injury , Metabolism , Pathology , Tetrahydroisoquinolines , PharmacologyABSTRACT
<p><b>AIM</b>To observe the effect of phenolic alkaloids of Menispermum dauricum (PAMD) on the hemodynamics, coronary circulation and oxygen metabolism of the myocardium in anesthetized dogs.</p><p><b>METHODS</b>In this study, the changes of LVSP, LVEDP and +/- dp/dtmax, the flow of coronary artery and myocardial energy metabolism were measured in anesthetized dog with PAMD or NS.</p><p><b>RESULTS</b>In the anesthetized dogs, compared with pre-treatment status, PAMD at 3.5 and 7.0 mg.kg-1 caused decreases in the left ventricular systolic pressure(LVSP), +/- dp/dtmax, heart rate, the rate of oxygen utilization, the coronary and general peripheral resistance. It was found to increase myocardial oxygen and coronary flow. There were no significant change in the left ventricular end diastolic pressure (LVEDP).</p><p><b>CONCLUSION</b>PAMD can ameliorate hemodynamics, coronary circulation and myocardial metabolism.</p>