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1.
Article in Chinese | WPRIM | ID: wpr-921534

ABSTRACT

Objective To observe the role of ultrasound biomicroscopy(UBM)in two-year post-operative follow-up for primary open-angle glaucoma patients with modified CO


Subject(s)
Carbon Dioxide , Follow-Up Studies , Glaucoma, Open-Angle/surgery , Humans , Microscopy, Acoustic , Sclera/diagnostic imaging , Treatment Outcome
2.
Ann. afr. méd. (En ligne) ; 14(3): 4263-4268, 2021.
Article in French | AIM | ID: biblio-1292623

ABSTRACT

Contexte & objectif. Les données sur la prise en charge des épaules en rhumatologie sont très limitées. L'objectif de la présente étude était de décrire la place de l'échographie dans la prise en charge des épaules douloureuses en rhumatologie. Méthodes. C'était une étude transversale sur les scapulalgies, suivies en rhumatologie de l'hôpital de Dreux ; entre janvier et mars 2018. Tous les participants avaient réalisé une échographie. Résultats. Au total 40 patients ont été enrôlés. Leur âge moyen était de 61,7 ans, et le sex ratio M/F de 0,29. A l'examen clinique, un conflit sous acromial (67,6%) et un syndrome acromio-claviculaire (32,4 %) étaient souvent observés. Les manœuvres de Jobe (76,5%), Patte (20,6%), et Gerber (14,7%) étaient parfois positives. A l'échographie, on observait les lésions des tendons de la coiffe (82,5%), du long biceps (25%), une bursite sous acromio-deltoïdienne (BSAD : 55%), une arthropathie gléno-humérale (30%), acromio-claviculaire (30%) ou sterno-claviculaire (2,5%). Une infiltration sous échographie était réalisée dans 85% des cas : BSAD (67,5%), gaine du long biceps (7,5%), articulation acromio-claviculaire (7,5%) et sterno- claviculaire (2,5%). Conclusion. L'échographie est très importante dans la prise en charge des scapulalgies dans le département de rhumatologie de l'hôpital de Dreux. Elle permet un diagnostic précis et la réalisation des gestes échoguidées.


Context and objective. Knowledge on management of shoulder pain is limited. The objective of the study was to describe the place of ultrasound in the management of shoulder pain in the Rheumatology Department of Dreux Hospital. Methods. A cross-sectional study was conducted on patients attending Rheumatology Department for shoulder pain from January to March 2018. All participants performed an ultrasound. Results. The mean age of the 40 patients included in the study was 61.7 years and the sex ratio was 0.29. On clinical examination, subacromial conflict (67.6%) and acromioclavicular syndrome (32.4%) were often observed. The Jobe (76.5%), Patte (20.6%), and Gerber (14.7%) tests were sometimes positive. Ultrasound lesions involved the rotator cuff (82.5%), subacromio-deltoid bursitis (55%), the long biceps tendon (25%), the glenohumeral joint (30%), the acromioclavicular joint (30%), or the sternoclavicular joint (2.5%). Ultrasound-guided infiltration was performed in 85% of cases. It concerned subacromio-deltoid bursitis (67.5%), long biceps sheath (7.5%), acromioclavicular joint (7.5%), and sterno-clavicular joint (2.5%). Conclusion. Ultrasound is very important in the management of shoulder pain in the Rheumatology Department of Dreux Hospital. It allows a precise diagnosis and the realization of echo-guided injections.


Subject(s)
Humans , Radiography, Interventional , Ultrasonography , Microscopy, Acoustic , Shoulder , Burkina Faso
3.
Arq. bras. oftalmol ; 82(5): 425-428, Sept.-Oct. 2019. graf
Article in English | LILACS | ID: biblio-1019439

ABSTRACT

ABSTRACT A rare case of bilateral congenital microcoria associated with antimetropia in a 47-year-old man is here described. The patient presented with a chief complaint of progressive vision loss in his right eye over the past five years. A slit-lamp examination and ultrasound biomicroscopy confirmed congenital microcoria and cataracts. Phacoemulsification was performed using an iris expansion device and the anterior capsule was stained using the "trypan down under" technique. Preoperative considerations, the surgical approach, and postoperative management are discussed.


RESUMO Um caso raro de microcoria congênita bilateral associada à antimetropia em um homem de 47 anos de idade é descrito aqui. O paciente queixava-se de perda visual progressiva em seu olho direito nos últimos 5 anos. Um exame com lâmpada de fenda e biomicroscopia ultrassônica confirmaram microcoria congênita e catarata. A facoemulsificação foi realizada usando dispositivo de expansão iriana, e a cápsula anterior foi corada através da técnica "trypan down under". Considerações pré-operatórias, abordagem cirúrgica e manejo pós-operatório são discutidos.


Subject(s)
Humans , Male , Adult , Middle Aged , Ophthalmic Solutions/administration & dosage , Atropine/administration & dosage , Cataract/complications , Cataract Extraction , Pupil Disorders/congenital , Phacoemulsification/methods , Pupil Disorders/surgery , Pupil Disorders/complications , Microscopy, Acoustic
4.
Arq. bras. oftalmol ; 81(3): 254-261, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-950458

ABSTRACT

ABSTRACT The diagnosis of angle-closure glaucoma secondary to iridociliary cysts is challenging and lacks compiled literature support. We present a rare case of bilateral angle-closure glaucoma associated with pseudoplateau iris due to multiple ciliary cysts and conducted a systematic review of the literature to find similar case reports published between November 2006 and November 2016. Only 19 case reports present treatment modalities, and most cases required more than one therapeutic approach for controlling the intraocular pressure. Pseudoplateau iris attributed to iridociliary cysts should be considered in the differential diagnosis of patients with narrow angles, particularly those with ocular hypertension and glaucoma, in which management is complex. In addition to gonioscopy, ultrasound biomicroscopy is considered the conclusive method for accurate diagnosis.


RESUMO O diagnóstico de glaucoma de ângulo fechado secundário a cistos iridociliares é desafiador e não possui suporte da literatura compilada. Apresentamos um caso bilateral raro de glaucoma de ângulo fechado associado à íris pseudoplateau devido a cistos ciliares múltiplos e realizamos uma revisão sistemática da literatura de relatos de casos similares publicados entre novembro de 2006 e novembro de 2016. Apenas 19 relatos de casos apresentaram as modalidades de tratamento e na maioria deles foi necessário mais de uma abordagem terapêutica para controlar a pressão intra-ocular. Íris pseudoplateau atribuída a cistos iridociliários deve ser considerada no diagnóstico diferencial de pacientes com ângulos estreitos, particularmente aqueles com hipertensão ocular e glaucoma, em que o manejo é complexo. Além da go nioscopia, a biomicroscopia ultra-sônica é considerada o método conclusivo para o diagnóstico correto.


Subject(s)
Humans , Male , Middle Aged , Uveal Diseases/complications , Glaucoma, Angle-Closure/etiology , Ciliary Body , Cysts/complications , Tonometry, Ocular , Glaucoma, Angle-Closure/diagnosis , Glaucoma, Angle-Closure/drug therapy , Microscopy, Acoustic , Tomography, Optical Coherence
5.
Arq. bras. oftalmol ; 81(3): 219-225, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-950445

ABSTRACT

ABSTRACT Purpose: We aimed to report the ocular manifestations observed in patients with psoriasis. Methods: Patients were included and referred to our ophthalmology clinic from dermatology clinics of Universidade do Estado do Pará between October 2013 and August 2014. Clinical interviews were conducted to identify relevant epidemiological data, clinical features, and treatment details, and data were recorded using the same protocol. Subsequent dermatological examinations were performed and disease severity was rated using the Psoriasis Area and Severity Index and the Dermatological Life Quality Index. Complete eye examination was conducted, including visual acuity, biomicroscopy, tonometry, fundoscopy, Schirmer I test, tear breakup time, rose bengal staining, ocular surface disease index, and glaucoma tests. Results: In total, we included 43 patients with psoriasis and 86 controls. Patients with psoriasis had statistically higher incidences of dry eye (16.28%), likely dry eye (32.56%), and blepharitis (16.28%). Furthermore, the rose bengal and ocular surface disease tests were more abnormal in patients with psoriasis (p<0.05). Conclusions: Patients with psoriasis should undergo regular eye exams, regardless of risk factors, to monitor for the progression of symptomatic or asymptomatic ocular manifestations.


RESUMO Objetivos: Relatar as manifestações oculares observadas em pacientes com psoríase atendidos no Ambulatório de Dermatologia da X e encaminhados ao Y, no período de outubro de 2013 a agosto de 2014. Métodos: A amostra foi constituída por um grupo composto por 43 pacientes com psoríase e um grupo controle com 86 pacientes sem psoríase. Foi realizada uma entrevista clínica com dados epidemiológicos, aspectos clínicos da doença e terapia empregada, sendo todas as informações registradas em protocolo próprio. Posteriormente, realizou-se o exame dermatológico, no qual foi avaliado o índice de gravidade da Psoríase por área (PASI) e índice dermatológico de qualidade de vida (DLQI), e o exame oftalmológico completo, incluindo: Acuidade Visual, Biomicroscopia, Tonometria, Fundoscopia, Teste de Schirmer I, Tempo de Ruptura do Filme Lacrimal (TBUT), rosa bengala, índice de doença da superfície ocular (OSDI) e exames para glaucoma. Resultados: Observou-se que nos pacientes com psoríase houve frequência estatisticamente maior de envolvimento ocular, como olho seco (16,28%), provável olho seco (32,56%) e blefarite (16,28%). Além disso, os valores do rosa bengala e do OSDI apresentaram-se mais alterados nos pacientes com psoríase (p<0,05). Conclusão: Dessa forma, sugere-se que esses pacientes realizem exames oftalmológicos periódicos, já que as manifestações oculares podem progredir sem sintomatologia e ocorrer independentemente de fatores de risco.


Subject(s)
Humans , Male , Female , Middle Aged , Psoriasis/complications , Eye Diseases/etiology , Tonometry, Ocular , Severity of Illness Index , Visual Acuity , Case-Control Studies , Microscopy, Acoustic , Eye Diseases/diagnosis
6.
Article in Korean | WPRIM | ID: wpr-738545

ABSTRACT

PURPOSE: We report a case of late-onset capsular block syndrome, which resulted in a misdiagnosis of intraocular lens (IOL) opacity. CASE SUMMARY: A 59-year-old man visited our clinic with reduced visual acuity in the right eye from 1 year prior. He had undergone uncomplicated bilateral cataract surgery by phacoemulsification with IOL implants at another hospital 10 years before. There was no specific history with the exception of hypertension. After being diagnosed in the ophthalmology clinic with IOL degeneration and opacity in the right eye, he was referred to our hospital for IOL replacement. Upon examination, his right uncorrected visual acuity was 0.06 and intraocular pressure was 22 mmHg. The refractive error could not be checked due to IOL opacity. Slit-lamp microscopy revealed a cloudy, milky IOL. Anterior-segment optical coherence tomography of the right eye showed retention of a highly reflective material in the lens capsule behind the IOL. Posterior capsule enlargement of the right eye was confirmed on ultrasound biomicroscopy. After neodymium-doped yttrium aluminium garnet (Nd:YAG) laser capsulotomy was performed, the homogeneous space disappeared and the eye recovered normal visual acuity. CONCLUSIONS: Capsular block syndrome is a rare complication that can occur shortly (1 day to 2 days) after cataract surgery. Late-onset capsular block syndrome, which occurs 10 years after surgery differs from typical clinical manifestations. Thus, capsular block syndrome is an important consideration upon the presentation of opacification due to IOL degeneration.


Subject(s)
Cataract , Diagnostic Errors , Humans , Hypertension , Intraocular Pressure , Lenses, Intraocular , Microscopy , Microscopy, Acoustic , Middle Aged , Ophthalmology , Phacoemulsification , Refractive Errors , Tomography, Optical Coherence , Visual Acuity , Yttrium
7.
Arq. bras. oftalmol ; 80(5): 327-329, Sept.-Oct. 2017. graf
Article in English | LILACS | ID: biblio-888150

ABSTRACT

ABSTRACT We report a case of a 49-year-old female who presented to the emergency department with blurred vision and vomiting, hours after taking two tablets of 250 mg of acetazolamide. The anterior chamber was bilaterally flat, with normal intraocular pressure in both eyes. An ultrasound biomicroscopic (UBM) examination showed bilateral ciliary effusion and complete appositional angle closure in all quadrants. Acetazolamide-induced bilateral angle closure was diagnosed. Steroid and cycloplegic therapy were initiated, and acetazolamide was discontinued. The following day, the anterior chamber had regained its volume without substantial change in the effusion size. Three weeks later, complete resolution of the ciliary effusion was verified by means of a third UBM scan.


RESUMO Relatamos um caso de uma mulher de 49 anos que se apresentou ao departamento de emergência informando visão borrada e vômitos, horas após ter tomado dois comprimidos de 250 mg de acetazolamida. A câmara anterior era bilateralmente plana com pressão intraocular normal em ambos os olhos. Um exame de biomicroscopia ultrassônica (UBM) mostrou efusão ciliar bilateral e fechamento completo do ângulo aposicional em todos os quadrantes. O bloqueio angular bilateral induzido por acetazolamida foi diagnosticado. O tratamento com esteróides e cicloplégicos foi iniciado e a acetazolamida foi descontinuada. No dia seguinte, a câmara anterior recuperou seu volume sem alterações substanciais no tamanho da efusão. Três semanas depois, a resolução completa da efusão ciliar foi verificada por meio de uma terceira biomicroscopia ultrassônica.


Subject(s)
Humans , Female , Middle Aged , Carbonic Anhydrase Inhibitors/adverse effects , Microscopy, Acoustic/methods , Anterior Chamber/drug effects , Anterior Chamber/diagnostic imaging , Acetazolamide/adverse effects , Myopia/chemically induced , Steroids/therapeutic use , Treatment Outcome , Intraocular Pressure , Mydriatics/therapeutic use , Myopia/drug therapy , Myopia/diagnostic imaging
8.
Arq. bras. oftalmol ; 80(4): 266-267, July-Aug. 2017. graf
Article in English | LILACS | ID: biblio-888134

ABSTRACT

ABSTRACT We report the case of a 46-year-old female patient who presented with bilateral acute angle closure during the course of dengue infection. Dengue diagnosis was confirmed by serological tests. The patient had plateau iris configuration identified by ultrasound biomicroscopy. This is the second reported case with this condition during dengue fever and the first to describe the characteristics of the angle.


RESUMO Os autores relatam o caso de uma mulher de 46 anos que teve fechamento agudo de ângulo bilateral durante a infecção por dengue. O diagnóstico de dengue foi confirmado por testes sorológicos. A paciente teve configuração de íris em platô confirmada pela biomicroscopia ultrassônica. Esse é o segundo caso descrevendo essa condição durante um episódio de dengue e o primeiro a descrever as características do ângulo.


Subject(s)
Humans , Female , Middle Aged , Glaucoma, Angle-Closure/etiology , Dengue/complications , Glaucoma, Angle-Closure/diagnostic imaging , Severe Dengue/complications , Microscopy, Acoustic
9.
Article in English | WPRIM | ID: wpr-26623

ABSTRACT

PURPOSE: To introduce a new convenient and accurate method to measure the angle kappa using ultrasound biomicroscopy (UBM) and corneal topography. METHODS: Data from 42 eyes (13 males and 29 females) were analyzed in this study. The angle kappa was measured using Orbscan II and calculated with UBM and corneal topography. The angle kappa of the dominant eye was compared with measurements by Orbscan II. RESULTS: The mean patient age was 36.4 ± 13.8 years. The average angle kappa measured by Orbscan II was 3.98°± 1.12°, while the average angle kappa calculated with UBM and corneal topography was 3.19°± 1.15°. The difference in angle kappa measured by the two methods was statistically significant (p < 0.001). The two methods showed good reliability (intraclass correlation coefficient, 0.671; p < 0.001). Bland-Altman plots were used to demonstrate the agreement between the two methods. CONCLUSIONS: We designed a new method using UBM and corneal topography to calculate the angle kappa. This method is convenient to use and allows for measurement of the angle kappa without an expensive device.


Subject(s)
Corneal Topography , Humans , Male , Methods , Microscopy, Acoustic , Ultrasonography
10.
Acta cir. bras ; 31(2): 103-110, Feb. 2016. graf
Article in English | LILACS | ID: lil-775563

ABSTRACT

PURPOSE: To track the regeneration process of lateral gastrocnemius due to a muscle laceration in rats, and to treatment with plateletrich plasma (PRP). METHODS: Ultrasound (40 MHz) images were used for measuring pennation angle (PA), muscle thickness (MT) and mean pixel intensity, along with claudication scores, of treated (PRPG) and non-treated (NTG) groups of rats. RESULTS: NTG showed a PA increase for the non-injured leg (p<0.05) and a tendency of MT to increase, whereas for PRPG there were no differences. There was a progressive reduction of the claudication score for the PRPG group throughout the entire period, with an immediate difference after seven days (p<0.05), whereas the NTG had a significant reduction only at day 28 (p<0.05). CONCLUSION: It was observed a compensatory hypertrophic response due to the overload condition imposed to healthy leg for NTG that did not occur in PRPG, suggesting an accelerated repair process of the injured leg due to treatment, anticipating its use.


Subject(s)
Animals , Female , Regeneration/physiology , Muscle, Skeletal/physiology , Muscle, Skeletal/physiopathology , Lacerations/therapy , Platelet-Rich Plasma , Random Allocation , Follow-Up Studies , Rats, Wistar , Muscle, Skeletal/diagnostic imaging , Lacerations/diagnostic imaging , Models, Animal , Microscopy, Acoustic/methods , Intravital Microscopy/methods , Intermittent Claudication/therapy
13.
Article in English | WPRIM | ID: wpr-51219

ABSTRACT

PURPOSE: Dry eye syndrome is commonly thought of as an inflammatory disease, and we have previously presented data showing the effectiveness of topical TNF-α blocker agents for the treatment of this condition. The purpose of this study was to investigate the effectiveness of the TNF-α blocking agent HL036337 compared to cyclosporine A for the treatment of dry eye induced inflammation in order to establish whether HL036337 represents a more effective method for suppressing inflammation. The efficacy of HL036337 and cyclosporine A was determined using an experimental murine dry eye model. METHODS: The TNF-α blocker HL036337 is a modified form of TNF receptor I. Using dry eye induced C57BL/6 mice (n = 45), corneal erosion was measured at day 4 and 7 after topical treatment with cyclosporine A or HL036337. To determine the effective treatment dose, 0.25, 0.5, 1, 2.5, and 5 mg/mL of HL036337 were topically administered twice per day to dry eye induced murine corneas for 1 week. RESULTS: The optimal concentration of the TNF-α blocker HL036337 for treatment of dry eye induced corneal erosion was determined to be 1 mg/mL. Dry eye induced corneal erosion was improved after 1 week with topically applied cyclosporine A and HL036337 at 1 mg/mL. CONCLUSIONS: HL036337 administered topically at 1 mg/mL effectively improved corneal erosion induced by dry eye. This finding may also suggest that inhibition of TNF-α can improve dry eye syndrome.


Subject(s)
Animals , Cornea/diagnostic imaging , Disease Models, Animal , Dose-Response Relationship, Drug , Dry Eye Syndromes/diagnosis , Female , Mice , Mice, Inbred C57BL , Microscopy, Acoustic , Ophthalmic Solutions/administration & dosage , Tumor Necrosis Factor-alpha/antagonists & inhibitors
14.
Rev. Soc. Colomb. Oftalmol ; 49(1): 67-74, 2016. ilus. graf.
Article in Spanish | LILACS, COLNAL | ID: biblio-910415

ABSTRACT

Propósito: Presentar los hallazgos ultrabiomicroscópicos observados en distintos tipos de catarata. Materiales y Métodos: Se correlacionan los hallazgos clínicos en la biomicroscopía con los obtenidos en la Biomicroscopía ultrasónica utilizando una sonda de 48 Mhz de Accutome. Resultados: Se incluyeron 32 ojos de 16 pacientes, de ellos 3 cataratas polares posteriores donde se observan imágenes ecodensas en el polo posterior con adelgazamiento de la cápsula sin compromiso del vítreo anterior. En los 15 casos de catarata corticonuclear se observan imágenes hiperecoicas en cortical anterior siendo mayor la densidad de la misma a mayor compromiso cortical con un núcleo poco ecoíco e incluso en 8 casos de opacidad netamente nuclear no se evidenciaron mayores alteraciones. En los 13 casos de catarata madura, con opacidades corticonucleares totales y blanquecinas se observaron imágenes de mayor densidad y homogéneas en la cortical anterior y posterior y si presentaron vacuolas o contenido acuoso se observaron áreas hipoecoicas inmediatamente subcapsulares anteriores. Por último, en 2 casos de catarata morganiana se observó engrosamiento irregular de la cápsula anterior con la presencia de un núcleo libre en el polo posterior del cristalino. En Conclusión, la Biomicroscopía ultrasónica ofrece detalles morfométricos del cristalino que son útiles en la planificación y abordaje quirúrgico de la catarata con la finalidad de minimizar las posibles complicaciones perioperatorias.


Purpose: To present the findings observed in different types of cataract by Ultrasonic Biomicroscopy. Materials and Methods: The clinical findings were correlated with those obtained by ultrasound using an ultrasonic probe of 48 Mhz by Accutome. Results: 32 eyes of 16 patients, 3 cases of posterior polar cataract with an image of echodense mass was observed at the posterior pole with slightly thinning of posterior capsule without involvement of anterior vitreous. In the 15 cases of moderate corticonuclear cataract hyperechoic images where observed in the anterior cortical region being of greater density as higher cortical compromise, with some or non-echoic activity in nucleus as observed in 8 cases of nuclear opacity without major cortical alterations. In the 13 cases of mature cataract, with total corticonuclear white opacities, compact images of high density and homogeneous were observed in anterior and posterior cortical region with some hypoechoic areas beneath the anterior capsule. Finally, in two cases of organian cataract irregular thickening of the anterior capsule with the presence of a free nucleus at the posterior pole of the lens was observed. In conclusion, the ultrasound ultrabiomicroscopy offers morphometric details of the lens which are useful in planning the surgical approach minimizing potential for intraoperative complications.


Subject(s)
Cataract , Diagnostic Techniques, Ophthalmological , Eye Diseases , Microscopy, Acoustic , Slit Lamp Microscopy
15.
Acta cir. bras ; 30(1): 13-23, 01/2015. tab, graf
Article in English | LILACS | ID: lil-735706

ABSTRACT

PURPOSE: Implement a laceration protocol of the rat lateral gastrocnemius (LG) and following-up its repair with ultrasound biomicroscopy (UBM), contractility tests and histology. METHODS: Sixty-three male Wistar rats were distributed into two groups. One, with sub-groups GI, GII and GIII (n=12), each containing right LG lacerated (n=6), control and sham (n=3) animals. LG muscles in GI, GII and GIII were inspected by UBM (40 MHz) immediately after, 14 and 28 days post-surgery and thereafter excised with four (GI), 14 (GII) and 28 (GIII) days post-surgery for histology. Animals in second group were distributed into right LG lacerated and control sub-groups. LG muscles in lacerated sub-group were submitted to contractility tests at four (n=8), 14 (n=8) and 28 (n=8) days post-surgery, while in the control sub-group (n=5) were submitted to contractility tests along the course of the experiments. RESULTS: Descriptive findings agreed between the lesion model, muscle repair, UBM images and histology. Contractility results for right LG were different (p<0.05) between control and injured muscle with four and 14 days post-surgery, at tetanic stimulating frequencies (50 and 70 Hz). CONCLUSION: A laceration protocol of the lateral gastrocnemius was implemented and ultrasound biomicroscopy, contractility and histology findings agreed regarding the following-up of injured muscle repair. .


Subject(s)
Animals , Male , Disease Models, Animal , Lacerations/physiopathology , Muscle, Skeletal/injuries , Regeneration/physiology , Lacerations/pathology , Lacerations , Microscopy, Acoustic/methods , Muscle Cells/physiology , Muscle Contraction/physiology , Muscle, Skeletal/physiopathology , Muscle, Skeletal , Rats, Wistar , Reproducibility of Results , Time Factors
16.
Article in English | WPRIM | ID: wpr-21887

ABSTRACT

BACKGROUND/AIMS: Smooth muscle cells (SMCs) characteristically express serum response factor (SRF), which regulates their development. The role of SRF in SMC plasticity in the pathophysiological conditions of gastrointestinal (GI) tract is less characterized. METHODS: We generated SMC-specific Srf knockout mice and characterized the prenatally lethal phenotype using ultrasound biomicroscopy and histological analysis. We used small bowel partial obstruction surgeries and primary cell culture using cell-specific enhanced green fluorescent protein (EGFP) mouse lines to study phenotypic and molecular changes of SMCs by immunofluorescence, Western blotting, and quantitative polymerase chain reaction. Finally we examined SRF change in human rectal prolapse tissue by immunofluorescence. RESULTS: Congenital SMC-specific Srf knockout mice died before birth and displayed severe GI and cardiac defects. Partial obstruction resulted in an overall increase in SRF protein expression. However, individual SMCs appeared to gradually lose SRF in the hypertrophic muscle. Cells expressing low levels of SRF also expressed low levels of platelet-derived growth factor receptor alpha (PDGFRalphalow) and Ki67. SMCs grown in culture recaptured the phenotypic switch from differentiated SMCs to proliferative PDGFRalphalow cells. The immediate and dramatic reduction of Srf and Myh11 mRNA expression confirmed the phenotypic change. Human rectal prolapse tissue also demonstrated significant loss of SRF expression. CONCLUSIONS: SRF expression in SMCs is essential for prenatal development of the GI tract and heart. Following partial obstruction, SMCs down-regulate SRF to transition into proliferative PDGFRalphalow cells that may represent a phenotype responsible for their plasticity. These findings demonstrate that SRF also plays a critical role in the remodeling process following GI injury.


Subject(s)
Animals , Blotting, Western , Fluorescent Antibody Technique , Gastrointestinal Tract , Heart , Humans , Mice , Mice, Knockout , Microscopy, Acoustic , Muscle Cells , Muscle, Smooth , Myocytes, Smooth Muscle , Parturition , Phenotype , Plastics , Polymerase Chain Reaction , Primary Cell Culture , Receptors, Platelet-Derived Growth Factor , Rectal Prolapse , RNA, Messenger , Serum Response Factor
17.
Arq. bras. oftalmol ; 77(6): 392-394, Nov-Dec/2014. graf
Article in English | LILACS | ID: lil-735800

ABSTRACT

We herein present a case with corneal overriding due to improper suturing of a full-thickness corneal laceration. There was a 2.5-mm difference between horizontal and vertical white-to-white measurements in the cornea. However, slit lamp examination failed to demonstrate the exact architecture of the laceration. Ultrasound biomicroscopy defined the wound edges thoroughly and confirmed the presence of corneal overriding. Six weeks after suture enhancement, the abnormal oval appearance of the cornea was absent and correct apposition of the corneal edges was seen on ultrasound biomicroscopy. Ultrasound biomicroscopy can be used in preoperative surgical planning of cases with complicated corneal lacerations. It can be used to adjust and enhance wound architecture in eyes with penetrating injury.


Apresentamos um caso com encavalamento corneano devido à sutura inadequada de uma laceração da córnea de espessura total. Houve uma diferença 2,5 mm entre as medidas do branco ao branco horizontais e verticais na córnea. No entanto, o exame da lâmpada de fenda não conseguiu demonstrar a arquitetura exata da laceração. A biomicroscopia ultrassônica definiu as bordas da ferida completamente e confirmou a presença de encavalamento da córnea. Seis semanas após a melhora da sutura, a aparência oval anormal da córnea havia desaparecido, e a correta aposição das bordas da córnea foi identificada na biomicroscopia ultrassônica. A biomicroscopia ultrassônica pode ser usada no planejamento cirúrgico pré-operatório de casos com lacerações corneanas complicadas. Ela pode ser utilizada para ajustar e melhorar a arquitetura da ferida em olhos com lesão penetrante.


Subject(s)
Adult , Humans , Male , Corneal Injuries/surgery , Lacerations/surgery , Postoperative Complications/surgery , Suture Techniques/adverse effects , Corneal Injuries , Eye Injuries, Penetrating/complications , Eye Injuries, Penetrating/surgery , Lacerations , Microscopy, Acoustic/methods , Postoperative Complications , Treatment Outcome
18.
Arq. bras. oftalmol ; 77(6): 382-387, Nov-Dec/2014. tab, graf
Article in English | LILACS | ID: lil-735805

ABSTRACT

Purpose: To describe quantitative and qualitative features of eyes with advanced bullous keratopathy assessed using ultrasound biomicroscopy, before and after anterior stromal puncture (ASP) or amniotic membrane transplantation (AMT) procedures to relieve chronic pain. Methods: The present descriptive comparative study included 40 eyes of 40 patients with chronic intermittent pain due to bullous keratopathy who were randomly assigned to one of the two treatments (AMT or ASP). Ultrasound biomicroscopy (Humphrey, UBM 840, 50 MHz transducer, immersion technique) was used, and a questionnaire about pain intensity was completed preoperatively and postoperatively at days 90 and 180, respectively. Exclusion criteria were age<18 years, presence of concurrent infection, ocular hypertension, and absence of pain. Results: In a 180-day follow-up, the AMT group exhibited mean central corneal thickness (CCT), 899.4 µm preoperatively and 1122.5 µm postoperatively (p<0.001); mean epithelial thickness (ET), 156.4 µm preoperatively and 247.8 µm postoperatively (p<0.001); and mean stromal thickness (ST), 742.9 µm preoperatively and 826.3 µm postoperatively (p=0.005). The ASP group exhibited mean CCT, 756.7 µm preoperatively and 914.8 µm postoperatively (p<0.001); mean ET, 102.1 µm preoperatively and 245.2 µm postoperatively (p<0.001); and mean ST, 654.6 µm preoperatively and 681.5 µm postoperatively (p<0.999). Correlations between CCT and pain intensity in the AMT group (p=0.209 pre- and postoperatively) and the ASP group (p=0.157 preoperatively and p=0.426 at the 180-day follow-up) were not statistically significant. Epithelial and stromal edema, Descemet’s membrane folds, epithelial bullae, and the presence of interface fluid were frequently observed qualitative features. Conclusion: CCT increased over time in both groups. The magnitude of CCT did not correlate with pain intensity in the sample studied. The presence of interface ...


Objetivo: Descrever as características quantitativas e qualitativas da biomicroscopia ultrassônica (UBM) em olhos com ceratopatia bolhosa avançada, antes e após os procedimentos de punção estromal anterior (ASP) ou transplante de membrana amniótica (AMT) para alívio de dor crônica. Métodos: Estudo comparativo descritivo incluindo 40 olhos de 40 pacientes com dor crônica intermitente devido a ceratopatia bolhosa, randomizados em duas modalidades de tratamento (AMT e ASP). Biomicroscopia ultrassônica (Humphrey, UBM 840, transdutor de 50 MHz, técnica de imersão) foi utilizada, e um questionário de avaliação da intensidade da dor foi aplicado no pré-operatório, e após 90 e 180 dias de pós-operatório. Critérios de exclusão foram: idade abaixo de 18 anos, presença de infecção, hipertensão ocular, e ausência de dor. Resultados: No seguimento de 180 dias, o grupo Transplante de membrana amniótica apresentou: média da espessura corneana central (CCT): 899,4 µm (pré), 1.122,5 µm (pós-operatório) (p<0,001); média da espessura epitelial (ET): 156,4 µm (pré), 247,8 µm (pós-operatório) (p<0,001); média da espessura estromal (ST): 742,9 µm (pré), 826,3 µm (pós-operatório) (p=0,005), e, grupo ASP apresentou: CCT média: 756.7 µm (pré), 914,8µm (pós-operatório) (p<0,001); ET média: 102,1 µm (pré), 245,2 µm (pós-operatório) (p<0,001); ST média: 654,6 µm (pré), 681.5 µm (pós-operatório) (p<0,999). A correlação entre intensidade da dor e espessura corneana central no grupo AMT (p=0,209 pré e pós-operatórios) e no grupo ASP (p=0,157 pré-operatório e p=0,426 aos 180 dias de seguimento) não foi significativa. Edema epitelial e estromal, dobras na membrana de Descemet, bolhas epiteliais, e presença de fluido na interface foram características qualitativas frequentemente observadas. Conclusão: A espessura corneana central aumentou ao longo do tempo em ambos os grupos. A magnitude da espessura corneana central não interfere na intensidade da dor ...


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Young Adult , Corneal Diseases/surgery , Corneal Diseases , Amnion/transplantation , Blister/surgery , Corneal Pachymetry , Corneal Stroma/pathology , Corneal Stroma/surgery , Corneal Stroma , Endothelium, Corneal/pathology , Endothelium, Corneal/surgery , Endothelium, Corneal , Eye Pain/surgery , Microscopy, Acoustic/methods , Pain Measurement , Postoperative Period , Preoperative Period , Punctures , Pain Management/methods , Palliative Care/methods , Statistics, Nonparametric , Surveys and Questionnaires , Time Factors , Treatment Outcome
20.
Arq. bras. oftalmol ; 77(1): 50-53, Jan-Feb/2014. graf
Article in English | LILACS | ID: lil-715555

ABSTRACT

Cyclodialysis is a relatively rare condition usually caused by ocular injury; however, it can also be caused iatrogenically during intraocular surgery. Hypotony maculopathy is the most important complication and the primary reason for visual loss. Clinical diagnosis using gonioscopy may be difficult, and ultrasound biomicroscopy (UBM) can be an alternative. There are different kinds of treatments, and the optimal one remains controversial. Here we describe a case of traumatic cyclodialysis with persistent ocular hypotony treated by direct cyclopexy, as illustrated by UBM performed before and after surgery.


Ciclodiálise é uma condição relativamente rara, geralmente devido a um trauma ocular, mas também pode ser causada iatrogenicamente como consequência de cirurgia intraocular. A maculopatia hipotônica é a complicação mais importante e a principal razão para a perda visual nessa situação. O diagnóstico clínico por gonioscopia pode ser difícil e a biomicroscopia ultrassônica (UBM) pode ser uma alternativa. Existem diferentes tipos de tratamentos e algumas controvérsias sobre a melhor opção. Neste relato, nós descrevemos um caso de ciclodiálise traumática com hipotonia ocular persistente tratado por cyclopexia direta avaliado por UBM antes e depois da cirurgia.


Subject(s)
Adult , Humans , Male , Ciliary Body/injuries , Ciliary Body/surgery , Eye Injuries/surgery , Ocular Hypotension/surgery , Ciliary Body , Eye Injuries , Gonioscopy , Microscopy, Acoustic , Ophthalmologic Surgical Procedures , Ocular Hypotension
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