Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 231
Filter
1.
Arq. Inst. Biol ; 86: e0232019, 2019. ilus
Article in English | ID: biblio-1024605

ABSTRACT

Rust, caused by the biotrophic fungus Olivea neotectonae, stands out as an important disease in teak plantations in Brazil. Considering the current importance of this rust and the need to obtain additional basic information about its causal agent to better disease management, the present study aimed to analyze the morphology of O. neotectonae using light microscopy and demonstrate the infection process in teak leaves using scanning electron microscopy techniques (SEM). The morphological characteristics observed using light microscopy were similar to those described in the literature for this species. SEM revealed that the infection structures of the pathogen penetrated the teak leaf through the stomata complex. This study provides the first microscopic evidence of the infection process of Olivea neotectonae in leaves of teak plants.(AU)


A ferrugem, causada pelo fungo biotrófico Olivea neotectonae, destaca-se como uma doença importante nas plantações de teca no Brasil. Considerando a importância atual dessa ferrugem e a necessidade de obter informações básicas adicionais sobre seu agente causal para melhor manejo da doença, o presente estudo teve como objetivo analisar a morfologia da O. neotectonae utilizando microscopia de luz e demonstrar o processo de infecção em folhas de teca por meio de técnicas de microscopia eletrônica de varredura (MEV). As características morfológicas observadas em microscopia de luz foram semelhantes às descritas na literatura para essa espécie. A MEV revelou que as estruturas de infecção do patógeno penetraram na folha de teca através do complexo estomático. Este estudo fornece a primeira evidência microscópica do processo de infecção de Olivea neotectonae em folhas de plantas de teca.(AU)


Subject(s)
Pest Control , Rainforest , Fungi , Plant Diseases , Microscopy, Electron/methods
2.
Mem. Inst. Oswaldo Cruz ; 112(8): 537-543, Aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-894863

ABSTRACT

BACKGROUND Silver nanoparticles (AgNPs) are increasingly being used in medical applications. Therefore, cost effective and green methods for generating AgNPs are required. OBJECTIVES This study aimed towards the biosynthesis, characterisation, and determination of antimicrobial activity of AgNPs produced using Pseudomonas aeruginosa ATCC 27853. METHODS Culture conditions (AgNO3 concentration, pH, and incubation temperature and time) were optimized to achieve maximum AgNP production. The characterisation of AgNPs and their stability were evaluated by UV-visible spectrophotometry and scanning electron microscopy. FINDINGS The characteristic UV-visible absorbance peak was observed in the 420-430 nm range. Most of the particles were spherical in shape within a size range of 33-300 nm. The biosynthesized AgNPs exhibited higher stability than that exhibited by chemically synthesized AgNPs in the presence of electrolytes. The biosynthesized AgNPs exhibited antimicrobial activity against Escherichia coli, P. aeruginosa, Salmonella typhimurium, Staphylococcus aureus, methicillin-resistant S. aureus, Acinetobacter baumannii, and Candida albicans. MAIN CONCLUSION As compared to the tested Gram-negative bacteria, Gram-positive bacteria required higher contact time to achieve 100% reduction of colony forming units when treated with biosynthesized AgNPs produced using P. aeruginosa.


Subject(s)
Humans , Silver/pharmacology , Colony Count, Microbial/methods , Metal Nanoparticles/chemistry , Gram-Negative Bacteria/metabolism , Gram-Negative Bacteria/ultrastructure , Gram-Positive Bacteria/drug effects , Anti-Bacterial Agents/biosynthesis , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Pseudomonas aeruginosa , Spectrophotometry , Microscopy, Electron/methods
3.
Braz. j. pharm. sci ; 52(3): 433-442, July-Sept. 2016. tab, graf
Article in English | LILACS | ID: biblio-828269

ABSTRACT

ABSTRACT Punarnava (Boerhaavia diffusa L.- Nyctaginaceae) is a promising drug to rejuvenate new cells in the body. It is well known in Ayurvedic medicine and locally called Tambadivasu. Superficially it is similar to other species of Boerhaavia and species of Trianthema and Sesuvium. Due to the minute morphological differences, the above plants are erroneously used in medicine as Punarnava, and at times on purpose as an adulterant. Therefore, it is necessary to highlight the anatomical features of Punarnava for proper identification of the medicinal plant species for local people and for scientific research. Due to the ambiguity in local names and similar apparent appearance, market samples of Punarnava are often adulterated with various species of Trianthema and Sesuvium. These adulterated samples contain neither the Punarnavine alkaloid, nor does it possess anisocytic stomata but possess paracytic stomata. Comparative study of stem anatomy showed two main characteristic differences. First, plenty of starch grains can be seen in both the ground parenchymatous tissues present in between successive cambia and xylem parenchyma of Punarnava which is not observed in species of Trianthema, and second, the phloem around the xylem of Punarnava root has semi-circular or eccentric patches, while that of Trianthema only has narrow strips. This study is focused on comparative SEM study of leaf morphologies and anatomy of leaf, stem, and root of Boerhaavia diffusa L., Trianthema portulacastrum L. and Sesuvium portulacastrum L.


Subject(s)
Comparative Study , Nyctaginaceae/anatomy & histology , Medicine, Ayurvedic , Microscopy, Electron/methods , Plants, Medicinal/anatomy & histology
4.
Reprod. clim ; 29(1): 37-40, jan.-abr. 2014.
Article in Portuguese | LILACS | ID: lil-743338

ABSTRACT

A microscopia eletrônica de espermatozoides é uma ferramenta complementar da análise seminal que pode contribuir na interpretação clínica da astenozoospermia grave e da teratozoospermia e na investigação de infertilidade idiopática. Reportamos um caso de paciente com varicocele, submetido à varicocelectomia, com análise seminal ultraestrutural por microscopia eletrônica.


Electron microscopy of sperm is a complementary tool to semen analysis that can contribute to the clinical interpretation of severe astenozoospermia, teratozoospermia and idiopathic infertility investigation. We report a patient with varicocele, submitted to varicocelectomy,with seminal ultrastructural analysis by electron microscopy.


Subject(s)
Humans , Male , Adult , Microscopy, Electron/methods , Spermatozoa , Varicocele/diagnosis
5.
J. bras. med ; 102(2)março-abril 2014. tab
Article in Portuguese | LILACS | ID: lil-712224

ABSTRACT

Diarreia aguda é a passagem de quantidade acima do normal de fezes amolecidas associada ao aumento do número de evacuações. No diagnóstico diferencial das diarreias agudas devem ser enfocados as infecções, as alergias alimentares, a intoxicação alimentar, o uso de medicações e a apresentação inicial de diarreia crônica. Dentre estas possíveis etiologias, especialmente em nosso meio, as causas infecciosas devem sempre vir à mente e constituir uma das primeiras opções na investigação diagnóstica. As infecções intestinais associadas a quadros diarreicos são a segunda causa de mortes de origem infecciosa em todo o mundo, com prevalência estimada de 3 a 5 bilhões de casos/ano. Os autores atualizam as novidades e peculiaridades a respeito do diagnóstico e dos tratamentos — geral e/ou específico — dos diferentes agentes associados à diarreia aguda infecciosa.


Acute diarrhea is the passage of above normal quantities of soft faeces also associated with increased bowel movements. Differential diagnosis of acute diarrhea should be focused on infections, food allergies, food poisoning, use of medications and the initial presentation of chronic diarrhea. Among these possible etiologies, given the environment we live in, infectious causes should always be taken into account and be one of the first options in diagnostic investigation. Intestinal infections associated with diarrheal frames are the second leading cause of infectious deaths worldwide, with an estimated to 3-5 billion cases/per year. In this review, the authors intend to review the new features and aspects concerning diagnosis and treatment — general and/or specific — of the different agents associated with acute infectious diarrhea.


Subject(s)
Humans , Male , Female , Diarrhea/diagnosis , Diarrhea/etiology , Diarrhea/therapy , Gastroenteritis/microbiology , Gastroenteritis/virology , Drug Utilization , Parasitic Diseases/diagnosis , Food Hypersensitivity , Foodborne Diseases , Fluid Therapy , Immunologic Tests , Bacterial Infections/diagnosis , Microscopy, Electron/methods , Thiazoles/therapeutic use
6.
Int. j. morphol ; 31(1): 184-188, mar. 2013. ilus
Article in Spanish | LILACS | ID: lil-676156

ABSTRACT

En peces, el nervio óptico es el encargado de transportar la información integrada por las células ganglionares de la retina hacia el tectum óptico, para que se generen imágenes acerca del entorno. El objetivo de este trabajo es describir morfométricamente el nervio óptico del tiburoncito (Ariopsis seemanni), para lo cual se utilizó la Microscopía óptica de Alta Resolución (MOAR), realizando cortes a 1 micra de espesor. El nervio óptico de A. seemanni presenta fibras mielínicas de diverso calibre, acompañadas de oligodendrocitos y astrocitos. El nervio está cubierto por las meninges, que presentan vasos sanguíneos y adipocitos. El nervio tiene un área total de 179604 ± 30163 µm2, diámetro de 478 ± 42 µm y un número total de fibras mielínicas de 22848 ± 4350, de las cuales la mayoría tiene un tamaño pequeño, que puede estar relacionado con una velocidad de conducción baja.


The optic nerve carries out the information integrated by retinal ganglion cells towards the optic tectum, so that surrounding environment images are generated. The main goal of this paper is to describe morphometricaly the Tete sea catfish (Ariopsis seemanni) optic nerve, using high resolution optical microscopy (HROM), on 1 micron thick sections. A. seemanni optic nerve does present myelinated nerve fibers, accompanied by oligodendrocytes and astrocytes. The nerve is covered by the meninges, with blood vessels and adipocites. The nerve has a transversal area of 179,604 ± 30163 mm2, a diameter of 478 ± 42 mm and 22848 ± 4350 myelinated fibers, most are small in size, which may be related to a low conduction velocity.


Subject(s)
Animals , Optic Nerve/ultrastructure , Catfishes/anatomy & histology , Microscopy, Electron/methods , Optic Nerve/anatomy & histology
7.
Int. j. morphol ; 31(1): 321-328, mar. 2013. ilus
Article in English | LILACS | ID: lil-676175

ABSTRACT

The aim of our research was to create an osteogenic unit in the skulls of athymic mice; however, the first challenge we faced was to find sufficient and adequate data that would allow us to determine the morphological, immunohistochemical and microtopographical characteristics that could be used as normality standards in athymic mice skulls and, hence, a reference in the event of achieving the formation of de novo bone using the osteogenic unit we proposed. Knowing the normal bone morphology in the skull of athymic mice was a necessary precondition to develop subsequently an osteogenic unit possessing the Osteogenesis, Osteoinduction and Osteoconductivity that could be compared versus those in the normal bone during its formations and remodeling processes. Therefore, we conducted a pilot study to determine bone morphological characteristics in the skull of athymic mice by means of specific histological staining: hematoxylin-eosin and Von Kossa, for osteoid tissue and mineralized bone, and Masson Tri-chrome for ossified areas. We also use immunohistochemistry to detect bone formation markers: alkaline phosphatase resulting from osteoblastic activity stimulation, type 1 collagen a bonematrix structural protein; Osteopontine, a protein specifically synthesized by osteoblasts that favors cell proliferation and remodeling in bone defects; Osteocalcine, a peptide hormone produced by osteoblasts during bone formation; and, Runx 2, a transcription factor expressed by stem cells which stimulates bone differentiation. Likewise, we used electron microscopy on the newly formed tissue to determine the presence of organic deposits, such as calcium, phosphate and magnesium in bone tissue.


Propusimos la realización de una unidad osteogénica a desarrollar en cráneo de ratones atímicos, Sin embargo, nos enfrentamos al reto de encontrar datos que nos determinaran cuales eran las características morfológicas, inmunohistoquímicas y micro-topográficas del cráneo de estos ratones atímicos, que nos sirvieran como referencia de normalidad y tener un punto de comparación, en caso de que pudiéramos lograr la formación de hueso de novo, a partir de la unidad osteogénica que propusimos. El objetivo, de conocer la morfología del hueso normal de cráneo de ratones atímicos, fue desarrollar posteriormente una unidad osteogénica que reuniera las características de Osteogénesis, Osteoinducción y Osteoconducción, y, compararlas contra las que tiene dicho hueso normal durante su proceso de formación y remodelación. Así, realizamos un estudio piloto donde establecimos características morfológicas de hueso del cráneo de ratones atímicos, a través de tinciones histológicas específicas, con hematoxilina-eosina y von Kossa para buscar tejido osteoide y hueso mineralizado y Tricrómico de Massón para observar zonas osificadas. Además, analizamos el tejido óseo a través de inmunohistoquímica, con la finalidad de buscar marcadores de formación ósea como fosfatasa alcalina que es resultado del estímulo de la actividad osteoblástica; colágena 1, la cual es una proteína estructural de la matriz ósea; osteopontina, proteína sintetizada específicamente por osteoblastos que favorece la proliferación celular y la remodelación en defectos óseos; osteocalcina hormona peptídica producida por los osteoblastos durante la formación ósea y Runx 2 Factor de transcripción expresado por las células progenitoras que estimula la diferenciación ósea. Además, sometimos el tejido óseo a microscopía electrónica para determinar la presencia de depósitos de compuestos como calcio, fósforo y magnesio.


Subject(s)
Animals , Rats , Skull/anatomy & histology , Skull/growth & development , Osteogenesis , Bone Regeneration , Rats, Nude , Immunohistochemistry , Microscopy, Electron/methods , Collagen Type I , Alkaline Phosphatase
8.
Egyptian Journal of Histology [The]. 2013; 36 (2): 312-320
in English | IMEMR | ID: emr-170245

ABSTRACT

Chemotherapy with cisplatin has adverse effects on spermatogenesis. Therefore, this work aimed at investigating the protective role of ginger against cisplatin-induced testicular toxicity in male albino rats. Twenty-four adult albino rats were used in this study. They were divided into three groups. The first group served as the control group; the second group was injected with cisplatin [12 mg/kg once]; and the third group was injected with cisplatin [12 mg/kg once] and then given ginger [310 mg/kg orally] for 26 days. Testicular specimens were processed for light microscopic examination using H and E. Other specimens were processed for electron microscopic examination. Cisplatin had damaging effects on the seminiferous tubules. Some areas of the tubules showed complete depletion of germ cells. Other areas showed some spermatogonia or primary spermatocytes. Sertoli cells showed a variable degree of degenerative changes in the form of destruction of cellular processes and cell junction. Interruption of the nuclear envelope of spermatids and loss of intercellular bridges were noticed. Treating with ginger resulted in normal Sertoli cells and cell junctions. The germ cells lining the tubules were more or less normal except for some intercellular vacuolations. The use of ginger has some protective effects on the testicular structure; hence, a larger number of experiments with higher doses of ginger or longer administration period could be beneficial for patients taking chemotherapeutic drugs


Subject(s)
Animals, Laboratory , Testis/pathology , Testis/ultrastructure , Microscopy, Electron/methods , Protective Agents , Ginger/drug effects , Treatment Outcome
9.
Assiut Medical Journal. 2013; 37 (1): 147-172
in English, Arabic | IMEMR | ID: emr-150542

ABSTRACT

Malathion is one of the organophosphorus insecticides widely used in agricultural and household applications to control pests. Actually, the studies of the effect of malathion on the adrenal%land are still limited, Vitamin C is the major water soluble antioxidant and free radical scavenger within the body. The aim of the work is to study the effect of chronic exposure to malathion on the adrenal gland. We also aim to study the possible protective effect of vitamin C in attenuating the nossible malathion induced changes. A total number of 30 adult male albino rats aged three months was used in the present study. Rats were randomly divided into three groups: l The first group [GI]: 10 rats were used as control 2- The second group [GH]: 10 animals were used as malathion treated group. 3- The third group [GUI]: 10 rats were used as malathion/vitamin C treated group. Commercial malathion was used, dissolved in distilled water and given orally by intragastric tube in a dose of 100 mg/kg/dayfor 2 months. Vitamin C was given orally by intragastric tube in a dose of 20 mg/WOgm/day for 2 months. After two months, the animals of the three groups were anaesthetized with ether inhalation and the suprarenal glands were dissected out and processed for light and electron microscopic examination. In malathion treated rats, there was cellulae disturbance in the arrangement of the adrenal gland. The cells of the cortex and medulla, showed irregular nuclei and apparent increase in the cytoplasmic vacuolation. Cortical and medullary blood capillaries were dilated and engorged with blood. Immunohistochemical staining for Caspase-3, showed many caspase-3 positive cells in the cortex and medulla. Ultrastructurally, degenerative changes were observed in the cortical and medullary cells in the form of cytoplasmic vacuolation, mitochondria! degeneration and increased lipid droplets. These changes were partially resolved by coadministration of vitamin C. Malathion had a harmful effect on adrenal gland so, it leads to impairment of its function in producing various hormones. This effect could be partially resolved, by concomitant administration of vitamin [C]. So, it is advisable to give vitamin [C] to those exposed to malathion


Subject(s)
Male , Animals, Laboratory , Adrenal Glands/ultrastructure , Microscopy, Electron/methods , Protective Agents , Ascorbic Acid , Treatment Outcome , Rats
10.
Rev. cuba. oftalmol ; 25(2): 202-211, oct.-dic. 2012.
Article in Spanish | LILACS | ID: lil-657924

ABSTRACT

Métodos: se realizó un estudio observacional, descriptivo y prospectivo. El universo lo constituyó 15 pacientes (15 ojos) sometidos a cirugías de catarata y vítreo de forma simultánea. El estudio se efectúo en el Instituto Cubano de Oftalmología Ramón Pando Ferrer de enero a noviembre de 2010...


Methods: a prospective, descriptive and observational research was carried out in Ramón Pando Ferrer Cuban Institute of Ophthalmology from January to November 2010. The sample was made up of 15 eyes from 15 patients, who had undergone cataract surgery and vitrectomy simultaneously...


Subject(s)
Middle Aged , Endothelium, Corneal/surgery , Cataract Extraction/adverse effects , Microscopy, Electron/methods , Vitrectomy/methods , Epidemiology, Descriptive , Observational Studies as Topic , Prospective Studies
11.
J. bras. med ; 100(3): 41-50, jul.-ago. 2012. tab
Article in Portuguese | LILACS | ID: lil-678749

ABSTRACT

Diarreia aguda é a passagem de quantidade acima do normal de fezes amolecidas associada ao aumento do número de evacuações. No diagnóstico diferencial das diarreias agudas devem ser enfocados as infecções, as alergias alimentares, a intoxicação alimentar, o uso de medicações e a apresentação inicial de diarreia crônica. Dentre estas possíveis etiologias, especialmente em nosso meio, as causas infecciosas devem sempre vir à mente e constituir uma das primeiras opções na investigação diagnóstica. As infecções intestinais associadas a quadros diarreicos são a segunda causa de mortes de origem infecciosa em todo o mundo, com prevalência estimada de 3 a 5 bilhões de casos/ano. Os autores atualizam as novidades e peculiaridades a respeito do diagnóstico e dos tratamentos - geral e/ou específico - dos diferentes agentes associados à diarreia aguda infecciosa


Acute diarrhea is the passage of above normal quantities of soft faeces also associated with increased bowel movements. Differential diagnosis of acute diarrhea should be focused on infections, food allergies, food poisoning, use of medications and the initial presentation of chronic diarrhea. Among these possible etiologies, given the environment we live in, infectious causes should always be taken into account and be one of the first options in dignostic investigation. Intestinal infections associated with diarrheal frames are the second leading cause of infectious deaths worldwide, with an estimated to 3-5 billion cases/per year. In this review, the authors inted to review the new features and aspects concerning diagnosis and treatment - general and/or specific - of the different agents associated with acute infectious diarrhea


Subject(s)
Humans , Male , Female , Diarrhea/diagnosis , Diarrhea/etiology , Diarrhea/therapy , Gastroenteritis/microbiology , Infections/complications , Parasitic Diseases/diagnosis , Fluid Therapy , Immunologic Tests , Loperamide/therapeutic use , Medical History Taking , Microscopy, Electron/methods , Physical Examination , Thiazoles/therapeutic use
12.
Egyptian Journal of Histology [The]. 2012; 35 (4): 686-696
in English | IMEMR | ID: emr-170222

ABSTRACT

Aging is believed to affect the structure and function of the enteric nervous system in the gastrointestinal tract. This work was designed to study the histological changes that might occur in the myenteric plexus of rat gastric fundus during aging. Thirty male albino rats were used in this study and divided equally into three groups: group A [which included 4-month-old rats], group B [which included 18-monthold rats], and group C [which included 24-month-old rats]. All the animals were anesthetized, and their stomachs were dissected out and processed for light and electron microscope examination. Also, immunohistochemical staining was carried out for the detection of protein gene product [PGP 9.5] and Bcl-2 proteins. The area% of myenteric ganglia was also determined. The results were analyzed statistically. With age, in group B [early senile], there was obvious neuronal loss and few gaps in the myenteric plexus, with a significant decrease in the number of PGP-positive neurons and moderate immunoreactions for the Bcl-2 protein. Glial cells had cytoplasmic vacuoles and nerve cells had peripheral condensation of heterochromatin in their nuclei. The myenteric plexus of the rats of group C [late senile] showed nerve cell bodies with apoptotic nuclei and cytoplasmic vacuolations. Many large cavities and eosinophillic cellular infiltration were observed within the neuropil. There was a marked apparent decrease in PGP 9.5-positive neurons and minimal immunoreactions for the Bcl-2 protein. Also, the area of myenteric ganglia increased with advancing age. In the current study, it was found that the myenteric plexus of the gastric fundus showed histological and immunohistochemical changes during aging, which may potentially be an explanation for the gastrointestinal tract disturbance in elderly individuals


Subject(s)
Animals, Laboratory , Gastric Fundus/ultrastructure , Histology , Microscopy, Electron/methods , Rats , Age Factors
13.
Egyptian Journal of Histology [The]. 2012; 35 (4): 790-804
in English | IMEMR | ID: emr-170231

ABSTRACT

The histological structure of the fallopian tube shows remarkable changes during pregnancy. Interstitial Cajal cell is known to play a key role in pacemaking motility in the gastrointestinal tract and the urinary tract. Its role in the motility of the fallopian tube is not fully explained. To describe the histological changes in the fallopian tube during early and late pregnancy, to shed light on the morphology of interstitial Cajal-like cells [ICLCs], and to localize their regional distribution in the fallopian tube in nonpregnant and pregnant rabbits. Thirty adult female rabbits were divided into three groups: group I [nonpregnant], group II [early pregnancy - day 4], and group III [late pregnancy - day 27]. The ampulla of the fallopian tube was excised and processed for histological, immunohistochemical, transmission, and scanning electron microscopy. In the nonpregnant group, it was found that the lining epithelium of the fallopian tube was composed of both ciliated and secretory cells. The ciliated cells were the major epithelial cell types; CD34-positive ICLCs were found maximally in the superficial zone of lamina propria, followed by musculosa, the deep portion of lamina propria, and least in serosa. The early pregnancy group showed a significant reduction in ciliated cells and an increase in secretory cells. In addition, there was a significant increase in the height of secretory cells and development of secretory organelles. There was a reduction in the number of CD34-positive ICLC in the superficial lamina propria compared with nonpregnant animals. The late pregnancy group showed an insignificant increase in ciliated cells and a decrease in secretory cells compared with group II. In addition, there was a decrease in the height of secretory cells and a reduction in their secretory organelles, but still more than that in group I. The structure of the fallopian tube showed histological changes during pregnancy. These changes represented a functional conditioning to the pregnancy state. ICLCs in the fallopian tube were prone to the same changes in their count and distribution


Subject(s)
Female , Animals, Laboratory , Rabbits , Fallopian Tubes/anatomy & histology , Interstitial Cells of Cajal , Fallopian Tubes/ultrastructure , Microscopy, Electron/methods
14.
Egyptian Journal of Histology [The]. 2012; 35 (1): 127-136
in English | IMEMR | ID: emr-126550

ABSTRACT

Nigella sativa seeds are commonly known as black seed or black cumin. It has been used for thousands of years as a spice and food preservative and also as a protective and curative remedy for numerous disorders. Our research aimed to study the possible toxic effect of different doses of N. sativa oil on the liver and kidney. Twenty-one adult male albino rats were used and divided equally into three groups. The first group was the control group. The second and third groups received the oil in two gradually increasing doses of 15 and 25 ml/kg, respectively, for 1 month. The animals were then sacrificed and samples of liver and kidney were taken and prepared for histological examination. In the kidney of group II there was epithelial shedding and necrosis of some cells of the proximal and distal convoluted tubules, but there was no effect on renal glomeruli. In contrast, in group III there was glomerular injury in the form of degeneration of the tuft of capillaries, ill-defined basement membrane, and destruction of endothelial cells, in addition to tubular necrosis. In group II there was minimal effect on the liver in the form of perivascular cellular infiltration; in group III was seen a markedly vacuolated foamy cytoplasm of hepatocytes, with dilated sinusoids and perivascular cellular infiltration. In conclusion, large doses of N. sativa oil have toxic effects on the histological structure of the kidney and to a lesser degree on the liver. Therefore, Nigella oil should be used in proper doses, and further studies on the effect of large doses of oil are recommended


Subject(s)
Male , Animals, Laboratory , Plant Oils/toxicity , Liver/pathology , Adrenal Cortex/pathology , Histology , Liver/ultrastructure , Adrenal Cortex/ultrastructure , Microscopy, Electron/methods , Rats , Male
15.
Egyptian Journal of Histology [The]. 2012; 35 (2): 304-314
in English | IMEMR | ID: emr-126566

ABSTRACT

Age-related sarcopenia represents a powerful risk factor for the loss of independence and physical disability in the elderly. Green tea contains a series of polyphenols called catechins and has been applied for disease prevention and treatment. To determine the effects of the intake of green tea on age-related changes in skeletal muscle. Thirty female albino rats were divided into three equal groups: group I [control adults] included 6-month-old rats, group II [aged]included 18-month-old rats, and group III [green tea treated] included 12-month-old rats that received green tea at a dose of 300mg/kg body weight in 1.5ml distilled water orally daily by a gastric tube for 6 months. Quadriceps muscle was dissected bilaterally and prepared for light and electron microscopical examination. Morphometric and statistical studies of the mean cross-sectional area of myofibers were carried out in the three studied groups. With increasing age, there were focal degeneration and atrophy, and significant decrease in the mean cross-sectional area of myofibers. The connective tissue framework showed increased collagen fiber deposition and deficient reticular fibers. Ultrathin sections showed areas of myofibrillar loss and mitochondria with destroyed cristae. Dense shrunken nuclei of satellite cells were observed. After treatment with green tea, normal-appearing myofibers, except for centrally located nuclei, were observed. The mean cross-sectional area of myofibers increased significantly compared with the aged group. Collagen and reticular fibers were relatively similar to those of the control. Proliferation of satellite cells was observed with restoration of normal myofiber ultrastructure. Good regenerative outcome was observed in aged skeletal muscle after the intake of green tea


Subject(s)
Female , Animals, Laboratory , Aged , Muscle, Skeletal/ultrastructure , Microscopy, Electron/methods , Plant Extracts , Camellia sinensis , Treatment Outcome , Rats
16.
Int. j. morphol ; 29(3): 960-964, Sept. 2011. ilus
Article in English | LILACS | ID: lil-608689

ABSTRACT

Increasingly the use and convenience of electrical appliances in our daily lives are the cause of harmful effects caused by electromagnetic fields (EMF). The aim of this study was to research the effect of EMF on the ultrastructure of the heart in EMF exposed rats. In this study 45 male Sprague Dawley rats ranging in weight between 260 and 280 grams were used. The rats were divided into 3 groups, control (n:15), Sham (n:15) and EMF group (n: 15) and exposed for 14 days 3 hours per day; gauss levels at 2.5 were applied to the EMF group, while the sham group in the same environment in Plexiglas cage was kept for 14 days 3 hours per day without magnetic field exposure. Control group at 14/10 hours light dark cycle fed in normal cages for 14 days. After two weeks rats were sacrificed by 50mg/kg of Ketalar anesthesia and heart tissue fixed in 2.5 gluteraldehide. Routine follow up with electron microscopic assessment. Mitochondrial structures and cellular structures observed in all the groups were normal. Myofibrillar loss, dilatation of smooth endoplasmic reticulum, mitochondrial swelling or cristalysis was not observed. Intercalated disc degeneration and apoptosis of nucleus was not observed. Therefore, and as a result of our study we did not observe differences between control and EMF groups.


El uso y la comodidad de los aparatos eléctricos en nuestra vida cotidiana cada vez más son causa de efectos perjudiciales debido a los campos electromagnéticos(CEM).El objetivo de este estudio fue investigar el efecto de los CEM sobre la ultraestructura del corazón en ratas. Fueron utilizadas 45ratas Sprague Daw ley, con peso entre 260 y 280gramos. Las ratas fueron divididas en 3 grupos: control (n: 15); Sham (n:15), y grupo expuesto a CEM (n:15) durante 14 días,3 horas por día. Se aplicó niveles de 2,5gaussal grupo expuesto a CEM, mientras que el grupo de tratamiento simulado en el mismo entorno en jaulas plexiglás se mantuvo durante 14 días 3 horas día, sin exposición a campo electromagnético. Grupo control alimentado en jaulas normales durante 14 días con ciclo luz/oscuridad de 14/10. Al termino de dos semanas las ratas fueron sacrificadas por medio de anestesia Ketalar 50mg/kg y el tejido del corazón fijado engluteraldehido al 2,5. Se realizó seguimiento de rutina con correspondiente evaluación de microscopía electrónica. Las estructuras mitocondriales y celular es observadas en todos los grupos eran normales. No se observó pérdida miofibrilar, tampoco aumento del volumen mitocondrial ni dilatación del retículo endoplásmico lisoocristalysis. No se observó degeneración de los discosintercaladoso apoptosis de núcleo. Por lo tanto,y como resultado de nuestro estudio no encontramos diferencias entre los grupos control y CEM.


Subject(s)
Rats , Electromagnetic Fields , Myocytes, Cardiac/cytology , Myocytes, Cardiac/ultrastructure , Microscopy, Electron/methods , Rats, Sprague-Dawley/physiology
17.
Braz. j. microbiol ; 42(3): 1017-1029, July-Sept. 2011. ilus, tab
Article in English | LILACS | ID: lil-607532

ABSTRACT

Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indicated an optimum activity in pH 5 at 45ºC. Enzyme was stable in 55ºC for 20 min and at a pH range of 3-9 for 90 min at 25ºC. When the temperature was raised to 60ºC, it might affect the structure of enzymes lead to reduction of chitinase activity. Moreover, the Km and Vmax values for chitin were 8.3 mg/ml and 2.4 mmol/min, respectively. Additionally, the effect of some cations and chemical compounds were found to stimulate the chitinase activity. In addition, Iodoacetamide and Idoacetic acid did not inhibit enzyme activity, indicating that cysteine residues are not part of the catalytic site of chitinase. Finally, chitinase activity was further monitored by scanning electronic microscopy data in which progressive changes in chitin porosity appeared upon treatment with chitinase. This enzyme exhibited antifungal activity against Rhizoctonia solani, Bipolaris sp, Alternaria raphani, Alternaria brassicicola, revealing a potential application for the industry with potentially exploitable significance. Fungal chitin shows some special features, in particular with respect to chemical structure. Difference in chitinolytic ability must result from the subsite structure in the enzyme binding cleft. This implies that why the enzyme didn't have significant antifungal activity against other Fungi.


Subject(s)
Enzyme Activation , Fungicides, Industrial , Microscopy, Electron/methods , Chitin/analysis , Chitinases/analysis , Serratia marcescens , Methods , Methods , Virulence
18.
Int. j. morphol ; 29(1): 272-277, Mar. 2011. ilus
Article in English | LILACS | ID: lil-591986

ABSTRACT

It is widely described in the literature that diabetic patients present hearing impairment. Despite the histological alterations of the internal ear structures in these patients as well as in experimental models of diabetes, to the best of our knowledge, an histological evaluation of the vestibulocochlear nerve have not been performed. In the present study, ultrastructural alterations are described and compared between a spinal nerves and a cranial nerve in rats with chronic induced diabetes. Male Wistar rats (n = 12), fed with standard diet from the animal care facility at 42 days of age were used. Induced diabetic animals (n=6) were fasted for 12 hours prior to being injected intraperitoneally with streptozotocin (STZ - 60mg/kg) in a single dose. Control animals (n=6) received (0.01 mol/l citrate buffer, pH 4.5) vehicle alone. Ten weeks after STZ injection the animals were perfused intracardially with Karnovsky solution. Right and left vestibulocochlear nerves were dissected and histologically processed for epoxy resin embedding. Samples were imaged with the transmission electron microscope. Large myelinated fibers with morphological signs of axonal atrophy in the vestibulocochlear nerves were readily observed. These results suggest that chronic STZ-induced diabetes in rats caused alterations in the myelinated fibers and Schwann cells, compatible to the classic diabetes signs and symptoms. Morphological alterations of the vestibulocochlear nerve in diabetes is described for the first time and contributes information for a better understanding of why there are changes in hearing observed in diabetic patients.


Se ha descrito ampliamente en la literatura que los pacientes diabéticos presentan discapacidad auditiva. En estos pacientes, a pesar de las alteraciones histológicas de las estructuras del oído interno, así como en modelos experimentales de diabetes, que mejoran nuestro conocimiento, la evaluación histológica del nervio vestibulococlear no ha sido realizada. Se describen y comparan las alteraciones ultraestructurales entre un nervio espinal y uno craneal en ratas con diabetes crónica inducida. Fueron utilizadas 12 ratas Wistar machos, de 42 días de edad, alimentadas con dieta estándar. Los animales diabéticos inducidos (n = 6) se mantuvieron en ayuno por 12 horas antes de ser inyectados por vía intraperitoneal con estreptozotocina (STZ - 60mg/kg) en una sola dosis. Los animales control (n = 6) sólo recibieron inyección de 0.01 mol/l buffer, citrato pH 4,5. Diez semanas después de la inyección de STZ, los animales fueron perfundidos intracardiacamente con solución de Karnovsky. Los nervios vestibulococlear derecho e izquierdo fueron disecados y procesados histológicamente para ser incluidos en resina epoxy. Las muestras fueron estudiadas con microscopio electrónico de transmisión. Fueron observadas fácilmente, grandes fibras mielinizadas con signos morfológicos de atrofia axonal en los nervios vestibulococlear. Estos resultados sugieren que la diabetes crónica inducida por STZ en ratas causó alteraciones en las fibras mielínicas y células del neurilema, compatible, con los signos y síntomas clásicos de la diabetes. Alteraciones morfológicas del nervio vestibulococlear en la diabetes son descritas por primera vez, lo que aporta información para una mejor comprensión de por qué hay cambios en la audición en los pacientes diabéticos.


Subject(s)
Animals , Male , Adult , Diabetes Mellitus, Experimental/chemically induced , Vestibulocochlear Nerve , Vestibulocochlear Nerve/ultrastructure , Microscopy, Electron/methods , Cochlear Nerve/physiopathology , Rats, Wistar/physiology
20.
Rev. venez. oncol ; 22(2): 126-129, abr.-jun. 2010.
Article in Spanish | LILACS | ID: lil-574469

ABSTRACT

Los tumores neuroendocrinos en la glándula mamaria, representan menos del 2 por ciento de las lesiones malignas que se presentan en la mama, un 30 por ciento pueden ser metastásicos, principalmente de tumores carcinoides intestinales. Se presenta el caso de una paciente femenina 64 años con el antecedente de carcinoma de mama izquierda pT1N1Mo Estadio II A, se le practicó en el año 2000 cirugía preservadora, recibió tratamiento sistémico y radioterapia, presentando recaída local, histológicamente y por inmuhistoquímica, como tumor neuroendocrino, en mayo de 2008 se le realiza mastectomía simple izquierda. Actualmente viva y sin enfermedad. Los tumores neuroendocrinos pueden presentarse en localizaciones extra intestinales. El diagnóstico debe realizarse por histología y confirmado con técnicas de inmunohistoquímica, son tumores de baja agresividad biológica, no se presentan síntomas sistémicos por liberación de hormonas como en otras localizaciones, y el tratamiento debe basarse en el estadio clínico de la enfermedad al momento del diagnóstico.


Neuroendocrines in the mammary gland tumors represent less than 2 percent of malignant injuries, which 30 percent can be source metastatic, mainly tumors intestinal carcinoid. The clinical of a female patient case 64 years old are presented with the antecedent of pT1N1Mo Stadium II A left breast carcinoma who was practiced in the year 2000 sparing surgery, received systemic therapy and radiotherapy, featuring local relapse, histological and inmuhistochemestry, as neuroendocrines, practicing it in May of 2008 mastectomy left, currently living and without disease tumor. Neuroendocrines tumors can occur in extra intestinal in lung, uterine and less common in the mammary gland neck locations. The diagnosis must be made by histology and confirmed with Immunohistochemistry techniques, are tumors of low biological aggression, not have systemic symptoms by release of hormones as in other locations, and treatment must be based on the clinical stage of the disease at the time of diagnosis.


Subject(s)
Humans , Female , Middle Aged , Microscopy, Electron/methods , Endocrine Gland Neoplasms/diagnosis , Secretory Vesicles/physiology , Biopsy, Fine-Needle/methods , Diagnostic Imaging/methods , Neuroendocrine Tumors/pathology
SELECTION OF CITATIONS
SEARCH DETAIL