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1.
Int. j. odontostomatol. (Print) ; 14(4): 617-622, dic. 2020. graf
Article in English | LILACS | ID: biblio-1134548

ABSTRACT

ABSTRACT: This study examined the salivary pH, salivary lactate, and salivary IL-1 β responses from a high-intensity intermittent running test, and the influence of hygiene oral status on these biomarkers in elite adolescent basketball players. Forty-six adolescent players participated. Saliva sampling was taken before and 3 min after a high-intensity exercise (Yo-Yo Intermittent Recovery Test Level 1; Yo-Yo IR1). In order to quantify and classify the oral hygiene level, the athletes were submitted to a dental examination, and an adapted Simplified Oral Hygiene Index was applied. After the dental examination, the whole group was divided into good oral hygiene group (GHG) and poor oral hygiene group (PHG). The results of a two- way analysis of variance showed a significant interaction effect (P = 0.0003), group effect (P < 0.0001), and time effect (pre to post Yo-yo IR1; P < 0.0001) for salivary pH and for salivary lactate (interaction effect, P = 0.008; group effect, P < 0.000 1; time effect, P < 0.0001) with a lower salivary pH and a higher salivary lactate at pre and post-Yo-Yo IR1 for PHG, but no difference was observed for IL-1β. The data demonstrated that the high-intensity exercise led to a significant change in salivary pH and salivary lactate concentration of the basketball players, and that the oral hygiene status influenced these responses, with a greater change for those players showing a poor oral hygiene.


RESUMEN: Este estudio examinó las respuestas de pH salival, lactato salival e IL-1β salival de una prueba de carrera intermitente de alta intensidad, y la influencia del estado de higiene oral en los biomarcadores en jugadores adolescentes de baloncesto de élite. En el análisis participaron 46 jugadores adolescentes. Se tomó una muestra de saliva antes y 3 minutos después de un ejercicio de alta intensidad (Prueba de recuperación intermitente Yo-Yo Nivel 1; Yo-Yo IR1). Para cuantificar y clasificar el nivel de higiene oral, los atletas fueron sometidos a un examen dental y se aplicó un índice adaptado de higiene oral simplificado. Después del examen dental, el grupo se dividió en un grupo de buena higiene oral (GHG) y un grupo de mala higiene oral (PHG). Los resultados de un análisis de varianza mostraron un efecto de interacción significativo (P = 0.0003), efecto de grupo (P<0.0001) y efecto de tiempo (antes y después de Yo-yo IR1; P <0.0001) para el pH salival y para lactato salival (efecto de interacción, P = 0.008; efecto de grupo, P <0.0001; efecto de tiempo, P <0.0001) con pH salival más bajo y lactato salival más alto en IR1 pre y post YoY para PHG, pero no se observó una diferencia para IL-1β. Los datos demostraron que el ejercicio de alta intensidad genera un cambio significativo en el pH salival y el lactato de los jugadores de baloncesto, y que el estado de higiene oral influyó en estas respuestas, con un cambio mayor para aquellos jugadores que mostraron una mala higiene oral.


Subject(s)
Humans , Animals , Adolescent , Oral Hygiene/education , Basketball , Oral Health/education , Oral Hygiene/statistics & numerical data , Saliva , Brazil , Statistical Analysis , Lactic Acid , Cytosine , Exercise Test , Athletes , Microbiota , Mouth/microbiology
2.
Arq. bras. med. vet. zootec. (Online) ; 72(4): 1113-1121, July-Aug. 2020. tab, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1131513

ABSTRACT

A proximidade dos primatas não humanos (PNH) com o ser humano pode ser considerada um fator de risco para transmissão de bactérias entre essas duas populações. Neste estudo, foi investigada a microbiota anfibiôntica aeróbica oral e retal de calitriquídeos em um fragmento de Mata Atlântica localizado no Rio de Janeiro, Brasil, e foram realizados testes fenotípicos para detecção de bactérias multirresistentes nos isolados encontrados. Foram capturados 14 calitriquídeos e coletadas 21 amostras (14 de cavidade oral e sete de cavidade retal) em dois pontos da mata próximos às habitações humanas. As espécies mais frequentes, na cavidade oral, foram Klebsiella oxytoca (50,0%), K. pneumoniae (28,6%), Kluyvera ascorbata (21,4%) e Stenotrophomonas maltophilia (21,4%) e, na cavidade retal, K. pneumoniae (85,7%), Escherichia coli (28,6%) e Enterobacter spp. (42,9%). Todos os 48 isolados da família Enterobacteriaceae foram negativos para ESBL (betalactamase de espectro ampliado), mostrando-se não produtores da enzima nos dois métodos utilizados: disco-aproximação e método de detecção automatizado. Na pesquisa de ERC (enterobactérias resistentes a carbapenêmicos), esses mesmos isolados não apresentaram resistência aos antibióticos imipenem, meropenem e ertapenem. Todas as bactérias isoladas apresentam um potencial zoonótico, o que representa um risco à saúde pública e à conservação das espécies.(AU)


Proximity of nonhuman primates (NHP) to humans can be considered a risk factor for transmission of pathogens between these two populations. This study investigated the oral and rectal aerobic bacterial microbiota of marmosets in an anthropized area of the Atlantic Forest located in Rio de Janeiro, Brazil, and performed phenotypic tests for detection of multidrug-resistant bacteria. Twenty-one samples (14 from the oral cavity and seven from the rectum) were collected from 14 Callithrix sp. captured in two sites of the forest near human dwellings. The most frequent species identified from the oral cavity swabs were Klebsiella oxytoca (50.0%), K. pneumoniae (28.6%), Kluyvera ascorbata (21.4%) and Stenotrophomonas maltophilia (21.4%), whereas the species most commonly identified from the rectum swabs were K. pneumoniae (85.7%), Enterobacter spp. (42.9%) and Escherichia coli (28.6%). All isolates of family Enterobacteriaceae showed no extended spectrum ß-lactamase production by disk-diffusion and automated detection tests. In the search for carbapenem-resistant enterobacteriaceae these isolates presented no resistance to the imipenem, meropenem and ertapenem antibiotics. The isolate of Staphylococcus aureus was susceptible to oxacillin and the isolate of Enterococcus was susceptible to vancomycin. All isolated bacteria showed zoonotic potential, thus posing a risk to species conservation and public health.(AU)


Subject(s)
Humans , Animals , Rectum/microbiology , Callithrix/microbiology , Microbiota , Mouth/microbiology , Staphylococcus aureus , Brazil , Disease Transmission, Infectious , Stenotrophomonas maltophilia , Health Risk , Klebsiella oxytoca , Escherichia coli
3.
Int. j. odontostomatol. (Print) ; 14(1): 131-135, mar. 2020. tab, graf
Article in English | LILACS | ID: biblio-1056512

ABSTRACT

ABSTRACT: Oral Lichen planus (OLP) is one of the main inflammatory diseases of the oral mucosa that is considered as a potentially malignant disorder. The exact pathogenesis of OLP remains to be completely understood. However, presence of bacteria has been associated to the inflammatory response observed in OLP. Particularly, Helicobacter pylori a major etiological agent of gastrointestinal inflammatory diseases and risk factor for gastric cancer, has been associated to Lichen planus. Here we studied a group of Chilean patients if there is any association between the presence of Helicobacter pylori and the clinical manifestation of OLP. We found a significant difference between the patients positive for H. pylori and the age of OLP diagnosis, suggesting that oral H. pylori might induce the disease at an earlier age. However, we could not confirm a statistically significance between the presence of the bacteria and OLP.


RESUMEN: Liquen Plano Oral (LPO) es una enfermedad inflamatoria de la mucosa oral considerada como desorden potencialmente maligno. La patogénesis exacta de LPO es desconocida. Sin embargo, se ha asociado la presencia de bacterias como responsables de la inflamación observada en LPO. Particularmente, Helicobacter pylori (H. pylori), agente etiológico principal de enfermedades inflamatorias gastrointestinales y factor de riesgo de cáncer gástrico, ha sido asociado con LPO. Se estudió la posible asociación entre H. pylori y manifestaciones clínicas de LPO en un grupo de pacientes Chilenos. Se encontró diferencia significativa entre los pacientes positivos para H. pylori y la edad de diagnóstico de LPO, sugiriendo que H. pylori podría inducir la enfermedad a temprana edad. Sin embargo, no se pudo confirmar significancia estadística entre la presencia de esta bacteria y la presencia de displasia en LPO.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Helicobacter pylori/isolation & purification , Lichen Planus, Oral/physiopathology , Lichen Planus, Oral/microbiology , Mouth/microbiology , Saliva/microbiology , Chile , Gene Amplification , Statistics, Nonparametric , Nucleic Acid Amplification Techniques
4.
Rev. cuba. estomatol ; 56(4): e1159, oct.-dez. 2019. tab
Article in Portuguese | LILACS | ID: biblio-1093246

ABSTRACT

RESUMO Introdução: No Brasil, uma lista contendo 71 ervas medicinais foi divulgada pelo Ministério da Saúde com intuito de conduzir pesquisas e desenvolvimento de fitoterápicos para uso no Sistema Único de Saúde. Objetivo: Avaliar in vitro a atividade antimicrobiana de extratos de ervas medicinais sugeridos pelo Ministério da Saúde para utilização no Sistema Único de Saúde frente a bactérias orais. Métodos: Os extratos vegetais selecionados estavam na forma de óleo essencial (Eucalyptus globulus, Mentha piperita e Schinus terebinthifolius) ou tintura (Erythrina mulungu, Casearia sylvestris e Maytenus ilicifolia) e foram avaliados sobre Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557) e S. salivarius (ATCC 7073). Realizou-se o teste de difusão em ágar com confecção de poços e adição de 50 µL dos extratos. Após 48 h em estufa bacteriológica, os halos de inibição foram medidos através de paquímetro. Para determinação da concentração inibitória mínima e da concentração bactericida mínima, realizou-se a técnica de microdiluição em microplacas e de esgotamento, respectivamente. A concentração inibitória mínima correspondeu a menor diluição na qual se verificou ausência de crescimento bacteriano visível. O controle positivo utilizado foi Clorexidina 0,12 porcento. Todos os testes foram realizados em triplicata e analisados descritivamente. Resultados: O extrato de Maytenus ilicifolia apresentou halos de inibição discretamente maiores aos demais produtos. Erythrina mulungu apresentou a menor da concentração inibitória mínima frente S. mutans (2,81 mg/mL) entre as tinturas e Mentha piperita (9,00), entre os óleos essenciais. Erythrina mulungu e Mentha piperita foram os únicos extratos que apresentaram concentração bactericida mínima frente às cepas utilizadas. Conclusão: Os extratos vegetais apresentaram atividade antimicrobiana frente a microrganismos orais destacando Mentha piperita e Erythrina mulungu(AU)


RESUMEN Introducción: En Brasil, una lista de 71 hierbas medicinales fue divulgada por el Ministerio de Salud con la meta de llevar a cabo la investigación y desarrollo de medicinas basadas en hierbas para su uso en el Sistema Único de Salud. Objetivo: Evaluar la actividad antimicrobiana in vitro de extractos de hierbas medicinales sugeridas por el Ministerio de Salud para su uso en el Sistema Único de Salud contra las bacterias bucales. Métodos: Los extractos de plantas seleccionadas fueron en forma de aceite essencial (Eucalyptus globulus, Mentha piperita e Schinus terebinthifolius) o tintura (Erythrina mulungu, Casearia sylvestris e Maytenus ilicifolia) y se evaluaron en Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557) y S. salivarius (ATCC 7073). Se llevó a cabo la prueba de difusión en agar con la producción de los pozos y la adición de 50 µL de extractos. Después de 48 h en una incubadora bacteriológica, los halos de inhibición se midieron usando calibradores. Para determinar la concentración inhibitoria mínima y la concentración bactericida mínima, se empleó la técnica de microdilución en microplaca y el agotamiento, respectivamente. La concentración inhibitoria mínima correspondió a la dilución más baja en la que no hubo ningún crecimiento bacteriano visible. El control positivo usado fue de clorhexidina 0,12 por ciento. Todas las pruebas se realizaron por triplicado y se analizaron de manera descriptiva. Resultados: Los extractos de Maytenus ilicifolia presentaron los halos de inhibición ligeramente superior a otros productos. Erythrina mulungu mostró la más baja concentración inhibitoria mínima frente a S. mutans (2,81 mg/mL) entre lãs tinturas y Mentha piperita (9,00) entre los aceites esenciales. Los extractos de Erythrina y Mentha piperita fueron los únicos que mostraron concentración bactericida mínima frente a las cepas utilizadas. Conclusiones: Los extractos de plantas mostraron actividad antimicrobiana contra microorganismos bucales, entre los que se destacan Mentha piperita y Erythrina mulungu(AU)


ABSTRACT Introduction: A list of 71 medicinal herbs has been published by the Brazilian Ministry of Health with the purpose of studying and developing medicines obtained from herbs for their potential use in the Unified Health System. Objective: Evaluate the in vitro antimicrobial activity of extracts from medicinal herbs proposed by the Ministry of Health with a view to their possible use in the Unified Health System to combat oral bacteria. Methods: Extracts were obtained from the plants selected in the form of essential oil (Eucalyptus globulus, Mentha piperita and Schinus terebinthifolius) or tincture (Erythrina mulungu, Casearia sylvestris and Maytenus ilicifolia), and were tested against Streptococcus mutans (ATCC 25175), S. oralis (ATCC 10557) and S. salivarius (ATCC 7073). The agar diffusion test was performed by making wells and adding 50 µL of the extracts. After 48 h in a bacteriological incubator, the inhibition haloes were measured with calipers. Minimum inhibitory concentration and minimum bactericidal concentration were determined by microplate microdilution and depletion technique, respectively. The minimum inhibitory concentration corresponded to the lowest dilution, at which there was no visible bacterial growth. The positive control used was 0.12 percent chlorhexidine. All the tests were performed in triplicate and analyzed descriptively. Results: Maytenus ilicifolia extracts exhibited slightly higher inhibition haloes than the other products. Erythrina mulungu had the lowest minimum inhibitory concentration against S. mutans (2.81 mg/mL) among tinctures, and Mentha piperita (9.00) among essential oils. Erythrina and Mentha piperita extracts were the only two displaying minimum bactericidal concentration against the strains used. Conclusions: The study plant extracts displayed antimicrobial activity against oral microorganisms, particularly against Mentha piperita and Erythrina mulungu(AU)


Subject(s)
Humans , Plants, Medicinal/adverse effects , Anti-Infective Agents/therapeutic use , Mouth/microbiology
5.
Braz. j. biol ; 79(3): 432-437, July-Sept. 2019. tab, graf
Article in English | LILACS | ID: biblio-1001460

ABSTRACT

Abstract The aims of this research were: evaluate the chemical composition and the cytotoxicity of the Cuminum cyminum (cumin), Anethum graveolens (dill), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) essential oils, as well as their antifungal activity in vitro against ten Candida spp. isolates. The chemical composition of the oils was analyzed by means of gas chromatography coupled with mass spectrometry (GC/MS). The cytotoxicity assays were performed, using the cell proliferation reagent WST-1 in L929 mouse fibroblasts (20x103 well-1). The determinate the Minimum Inhibitory Concentration (MIC), was performed through the Broth Microdilution technique (CLSI). The chemical main components were the cuminaldehyde (32.66%) for cumin, carvone (34.89%) for the dill, trans-anethole (94.01%) for the anise and anethole (79.62%) for the fennel. Anise and fennel did not were cytotoxic in all the tested concentrations, however the cumin oil was cytotoxic in the concentration of 20 mg.mL-1 and the dill in the concentrations of 20 and 8 mg.mL-1. All yeasts were susceptible against the evaluated essential oils. Cumin presented the lowest MIC against yeasts. We concluded that all the essential oils presented inhibitory action against Candida spp., and C . cyminum, P. anisum and F. vulgare were not cytotoxic in the same minimum inhibitory concentrations for the fungi.


Resumo Os objetivos desta pesquisa foram: avaliar a composição química e a citotoxicidade dos óleos essenciais de Cuminum cyminum (cominho), Anethum graveolens (endro), Pimpinella anisum (erva-doce) e Foeniculum vulgare (funcho), bem como sua atividade antifúngica in vitro contra dez isolados de Candida spp.. A composição química dos óleos foi analisada por meio de cromatografia gasosa acoplada à espectrometria de massa (GC / MS). Os ensaios de citotoxicidade foram realizados, utilizando o reagente de proliferação celular WST-1 em fibroblastos de ratinho L929 (20x103 poço-1). A determinação da Concentração Inibitória Mínima (MIC) foi realizada através da técnica de microdiluição em caldo (CLSI). Os principais componentes químicos foram o cuminaldeído (32.66%) para cominho, carvona (34.89%) para o endro, trans-anetol (94.01%) para erva-doce e anetol (79.62%) para a funcho. O endro e a erva-doce não foram citotóxicos em todas as concentrações testadas, no entanto, o óleo de cominho foi citotóxico na concentração de 20 mg.mL-1 e o endro nas concentrações de 20 e 8 mg.mL-1. Todas as leveduras foram suscetíveis aos óleos essenciais avaliados. O cominho apresentou a menor CIM contra as leveduras. Concluímos que todos os óleos essenciais apresentaram ação inibidora contra Candida spp., e C. cyminum, P. anisum e F. vulgare não foram citotóxicos nas mesmas concentrações inibitórias mínimas para os fungos.


Subject(s)
Candida/drug effects , Oils, Volatile/pharmacology , Apiaceae/chemistry , Antifungal Agents/pharmacology , In Vitro Techniques , Microbial Sensitivity Tests , Gas Chromatography-Mass Spectrometry , Mouth/microbiology
6.
J. oral res. (Impresa) ; 8(1): 30-36, feb. 28, 2019. ilus, tab
Article in English | LILACS | ID: biblio-1145264

ABSTRACT

Objectives: To study the prevalence of methicillin resistant Staphylococcus aureus (MRSA) in saliva samples of pre-surgical oral squamous cell carcinoma (OSCC) patients along with their resistance pattern to other antibiotics. Methods: Saliva samples of OSCC patients were collected and processed for isolation of MRSA. Staphylococcus aureus isolates were primarily identified using standard microbiological methods like biochemical assays, specialized media and latex agglutination test. Confirmation of MRSA strains was done by growing the isolates on MRSA agar and by using PCR to amplify two MRSA specific genes. All the isolated Staphylococcus aureus strains were subjected to antibiotic sensitivity tests. Results: A total of 17 Staphylococcus aureus strains were isolated from 50 saliva samples of pre-surgical OSCC patients of which 13 were confirmed to be MRSA. These MRSA strains were also found to be mostly resistant to other commonly used antibiotics. Univariate analysis revealed that most patients with MRSA infections had a prior history of hospitalization and surgery. Also, it was confirmed that patients with other comorbidities and infections were more prone to having MRSA present in the saliva. Conclusion: The majority of Staphylococcus aureus isolates from the saliva of OSCC patients were MRSA, and were resistant to several other commonly used antibiotics.


Objetivos: Estudiar la prevalencia de Staphylococcus aureus resistente a la meticilina (MRSA) en muestras de saliva prequirúrgicas de pacientes con carcinoma oral de células escamosas (COCE) junto con su patrón de resistencia a otros antibióticos. Métodos: Se recolectaron muestras de saliva de pacientes con COCE y se procesaron para el aislamiento de SARM. Los aislamientos de Staphylococcus aureus se identificaron principalmente mediante métodos microbiológicos estándar, como los análisis bioquímicos, los medios especializados y la prueba de aglutinación con látex. La confirmación de las cepas de SARM se realizó cultivando los aislados en agar SARM y utilizando PCR para amplificar dos genes específicos de SARM. Todas las cepas aisladas de Staphylococcus aureus se sometieron a pruebas de sensibilidad a los antibióticos. Resultados: Se aislaron un total de 17 cepas de Staphylococcus aureus a partir de 50 muestras de saliva de pacientes prequirúrgicos con COCE, de los cuales solo se confirmó que 13 eran SARM. También se encontró que estas cepas de SARM son resistentes a otros antibióticos de uso común. El análisis univariado reveló que la mayoría de los pacientes con infecciones por SARM tenían antecedentes previos de hospitalización y cirugía. Además, se confirmó que los pacientes con otras comorbilidades e infecciones eran más propensos a las infecciones por SARM. Conclusión: la mayoría de los aislamientos de Staphylococcus aureusde la saliva de los pacientes con OSCC fueron MRSA y fueron resistentes a varios otros antibióticos de uso común.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Staphylococcus/isolation & purification , Drug Resistance, Microbial , Methicillin-Resistant Staphylococcus aureus/genetics , Squamous Cell Carcinoma of Head and Neck , Mouth/microbiology , Saliva , DNA, Bacterial/genetics , Prevalence , Anti-Bacterial Agents
7.
Rev. Soc. Bras. Med. Trop ; 52: e20180294, 2019. graf
Article in English | LILACS | ID: biblio-977115

ABSTRACT

Abstract INTRODUCTION: Goiás State, which is in the midwest region of Brazil, has several urban forests. This fact, along with the expansion of urban areas within the limits of Forest Conservation Units, increases the contact between humans and wildlife, such as capuchin monkeys. The impulsive behavior of these animals and the scarcity of food cause them to vigorously search for food, leading to direct encounters with Park visitors, which can result in scratches and bites and making them potential disseminators of pathogenic microorganisms. METHODS: Ten specimens of bearded capuchin monkey (Sapajus libidinosus) were captured at the Onofre Quinan Environmental Park in Anápolis, Goiás, Brazil. Samples were collected from the monkeys, and the bacteria and fungi present in the samples were isolated and identified. Then, the identified microorganisms were subjected to antibiotic susceptibility testing. RESULTS: A total of 111 bacteria and 12 fungi were isolated, including two strict anaerobic bacteria of the genus Peptostreptococcus, 109 facultative anaerobic bacteria, and 12 yeasts. Among the facultative bacteria, enterobacteria and Staphylococcus were common. Resistance to tetracycline and ampicillin antibiotics was detected in the enterobacteria, and resistance to tetracycline, erythromycin, and clindamycin was detected in the Staphylococci. The other strains were sensitive to all tested antimicrobials. Cefoxitin showed 100% efficacy in all isolated bacteria. CONCLUSIONS: For bites from capuchin monkeys, we recommend performing complete hygiene and antibiotic therapy, according to medical recommendations. Given the 100% effectiveness of cefoxitin, it should be considered for this type of injury, especially in the study region.


Subject(s)
Humans , Animals , Bites and Stings/microbiology , Cebus/microbiology , Microbiota/drug effects , Mouth/microbiology , Brazil , Microbial Sensitivity Tests , Mouth/drug effects
8.
Article in Spanish | LILACS | ID: biblio-1007328

ABSTRACT

Propolis is a substance manufactured by Apis mellifera and has been widely used in folk medicine due to its high concentration of bioactive compounds. The purpose of the following study was to characterize and evaluate in vitro the antimicrobial properties of propolis on clinical samples and ATCC strains. The chemical characterization of propolis presents a concentration of total polyphenols of 247 ± 9 mg EAG g-1 MS, flavones and flavonols 75± 4 mg EQ g-1 MS, flavanonones and flavanonols 118 ± 11 EP g-1 MS. HPLC-DAD identified apigenin, galangin, phenethyl ester of caffeic acid and pinocembrin, in addition to 16 compounds by HPLC MS/MS. Chilean propolis is a natural antimicrobial, showing effectiveness in strains ATCC Staphylococcus aureus, Candida albicans, Trichophyton rubrum and clinical samples of Staphylococcus aureus unlike Escherichia coli. These results demonstrate the antimicrobial effectiveness of the synergy of compounds present in propolis against different human pathogens.


El propóleos es una substancia fabricada por Apis mellifera y ha sido utilizado ampliamente en la medicina popular debido a su alta concentración de compuestos bioactivos. El propósito del siguiente estudio fue caracterizar y evaluar in vitro las propiedades antimicrobianas del propóleos sobre muestras clínicas y cepas ATCC. La caracterización química de propóleos presenta una concentración de polifenoles totales de 247 ± 9 mg EAG g-1 de MS, flavonas y flavonoles 75 ± 4 mg EQ g-1 de MS, flavanononas y flavanonoles 118 ± 11 EP g-1 de MS. Mediante HPLC-DAD se identificó apigenina, galangina, fenetil éster del ácido cafeico y pinocembrina, además de 16 compuestos mediante HPLC MS/MS. El propóleos chileno es un antimicrobiano natural, observándose efectividad en cepas ATCC Staphylococcus aureus, Candida albicans, Trichophyton rubrum y muestras clínicas de Staphylococcus aureus a diferencia de Escherichia coli. Estos resultados demuestran la efectividad antimicrobiana de la sinergia de compuestos presentes en el propóleos ante diferentes patógenos humanos.


Subject(s)
Humans , Propolis/pharmacology , Staphylococcus aureus/drug effects , Candida albicans/drug effects , Escherichia coli/drug effects , Anti-Infective Agents/pharmacology , Pharynx/microbiology , Propolis/chemistry , Trichophyton/drug effects , Flavonoids/analysis , Microbial Sensitivity Tests , Apis mellifica , Chile , Chromatography, High Pressure Liquid , Escherichia coli/drug effects , Gas Chromatography-Mass Spectrometry , Anti-Infective Agents/chemistry , Mouth/microbiology
9.
Braz. j. biol ; 78(4): 644-652, Nov. 2018. tab
Article in English | LILACS | ID: biblio-951608

ABSTRACT

Abstract The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours. The morphology identification was performed through macroscopic and microscopic characterization, the CHROMagar Candida medium and the VITEK® system Yeast Biochemical Card (bio Mérieux SA, France). The results showed a colonization of Candida spp. in 85.7% the hospitalized individuals, where the species found were C. albicans (60%), C. tropicalis (23.4%), C. krusei (3.3%) and Candida spp. (13.3%). In the non-hospitalized individuals the colonization by Candida spp was 47.1%, and the species found were: C. albicans (45.5%), C.krusei (9.1%), C. guilliermondii (9.1% %), C. tropicalis (3.0%), C. famata (3.0%) and Candida spp. (30.3%). In spite of their presence in oral cavity in both groups, Candida spp. was more frequently isolated in hospitalized individuals, who were 6.73 times more likely to have this fungus in the oral cavity and were 3.88 times more likely to have Candida albicans.


Resumo O objetivo deste estudo foi avaliar a frequência de espécies de Candida entre uma população de indivíduos não-hospitalizados e hospitalizados. Para isto, amostras de saliva foram coletadas através de swabs estéreis, umedecidas em água de peptona e friccionadas na cavidade bucal de 140 indivíduos, dos quais 70 eram pacientes internados em uma Clínica Médica de um Hospital Escola e os outros 70 eram indivíduos não hospitalizados sem contato com ambiente hospitalar. Todas as amostras de saliva foram plaqueadas em ágar Sabouraud dextrose adicionadas de cloranfenicol e incubadas a 36 °C durante 48 horas. A identificação morfológica foi realizada através da caracterização macroscópica e microscópica, com o meio CHROMagar Candida e do sistema VITEK® Biochemical Card (bio Mérieux SA, França). Os resultados mostraram uma colonização de Candida spp. em 85,7% dos indivíduos hospitalizados, onde as espécies encontradas foram: C.albicans (60%), C. tropicalis (23,4%), C. krusei (3,3%) e Candida spp. (13,3%). Nos indivíduos não-hospitalizados a colonização por Candida spp foi de 47,1%, e as espécies encontradas foram: C. albicans (45,5%), C. krusei (9,1%), C. guilliermondii (9,1%), C. tropicalis (3,0%), C. famata (3,0%) e Candida spp. (30,3%). Apesar de sua presença na cavidade oral em ambos os grupos, Candida spp. foi mais freqüentemente isolada em indivíduos hospitalizados, que foram 6,73 vezes mais propensos a ter este fungo na cavidade oral e foram 3,88 vezes mais propensos a ter Candida albicans.


Subject(s)
Humans , Male , Female , Middle Aged , Outpatients/statistics & numerical data , Candida/isolation & purification , Candidiasis/diagnosis , Candidiasis/microbiology , Inpatients/statistics & numerical data , Saliva/microbiology , Candida/classification , Candida/growth & development , Colony Count, Microbial , Culture Media , Mouth/microbiology
10.
An. bras. dermatol ; 93(3): 356-361, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-949881

ABSTRACT

Abstract: BACKGROUND: Candidiasis is the most common opportunistic fungal infection of the oral cavity caused by fungi of the genus Candida and usually associated with immunosuppressed individuals. OBJECTIVES: To evaluate the presence of oral candidiasis and identify the presence of Candida spp. in liver transplant recipients and assess the association between the presence of the fungus and sociodemographic variables, dietary habits and environmental exposure. METHODS: A cross-sectional study was performed with 49 patients who had undergone liver transplants at Hospital São Vicente de Paulo in Passo Fundo - RS. Patient information was collected to obtain sociodemographic data, eating habits and environmental exposure. Fungal infections were screened by oral clinical examination and the presence of Candida spp by the collection of oral samples with a sterile swab, seeded in Sabouraud Dextrose Agar, incubated at 25°C and observed at 48 hours. To identify Candida albicans, the germ tube test was performed. RESULTS: In 49 patient samples, 39% had the yeast of the genus Candida isolated and, of these patients, 12% had candidiasis, 66% of atrophic type and 34% pseudomembranous. Eleven yeast species were (58%) Candida non-albicans and eight (42%) Candida albicans. STUDY LIMITATIONS: The present study presents as a limitation the inclusion of patients in different stages of immunosuppression. CONCLUSION: The high incidence of Candida non-albicans in the oral cavity of transplant patients with a long period of transplantation is warning to a more effective control of the health of these individuals, especially those with older age.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Young Adult , Opportunistic Infections/microbiology , Candida/isolation & purification , Candidiasis, Oral/diagnosis , Liver Transplantation , Mouth/microbiology , Candida/classification , Candidiasis, Oral/pathology , Cross-Sectional Studies , Immunocompromised Host , Antifungal Agents/therapeutic use
11.
J. appl. oral sci ; 26: e20170516, 2018. tab, graf
Article in English | LILACS, BBO | ID: biblio-954499

ABSTRACT

Abstract Objective The objective of this study was to clarify differences in bacterial accumulation between gastrointestinal cancer patients who underwent severely invasive surgery and those who underwent minimally invasive surgery. Material and Methods We performed a preliminary investigation of gastrointestinal cancer patients who were treated at the Department of Surgery, Takarazuka Municipal Hospital, from 2015 to 2017 (n=71; 42 laparoscopic surgery, 29 open surgery) to determine changes in bacterial numbers at different sites of the oral cavity (tongue dorsum, gingiva of upper anterior teeth, palatoglossal arch), as well as mouth dryness and tongue coating indices. Specifically, patients received professional tooth cleaning (PTC), scaling, tongue cleaning, and self-care instruction regarding tooth brushing from a dental hygienist a day before the operation. Professional oral health care was also performed by a dental hygienist two and seven days after surgery. Oral bacteria numbers were determined using a bacterial counter with a dielectrophoretic impedance measurement method. Results The number of bacteria at all three examined sites were significantly higher in the open surgery group when compared to the laparoscopic surgery group on the second postoperative day. Relevantly, bacterial count in samples from the gingiva of the upper anterior teeth remained greater seven days after the operation in patients who underwent open surgery. Furthermore, the dry mouth index level was higher in the open surgery group when compared to the laparoscopic surgery group on postoperative days 2 and 7. Conclusions Even with regular oral health care, bacterial numbers remained high in the upper incisor tooth gingiva in gastrointestinal cancer patients who received open surgery. Additional procedures are likely needed to effectively reduce the number of bacteria in the gingival area associated with the upper anterior teeth.


Subject(s)
Humans , Male , Female , Adult , Aged , Aged, 80 and over , Oral Health , Perioperative Care , Gastrointestinal Neoplasms/surgery , Gastrointestinal Neoplasms/microbiology , Mouth/microbiology , Postoperative Period , Time Factors , Body Temperature , C-Reactive Protein/analysis , Laparoscopy , Statistics, Nonparametric , Preoperative Period , Bacterial Load , Gastrointestinal Neoplasms/pathology , Leukocyte Count , Middle Aged , Neoplasm Staging
12.
Braz. dent. j ; 28(4): 423-427, July-Aug. 2017. tab
Article in English | LILACS | ID: biblio-888675

ABSTRACT

Abstract The aim of this study was to isolate Enterobacteria and Pseudomonas from the oral cavity of hospitalized newborns (NB) and determine their prevalence and the sensitivity profile to most commonly used antibiotics for this age group. Samples from the oral cavity of NB from 24 to 48 h age were collected using swabs. The samples were inoculated on MacConkey agar, incubated and the colonies counted and identified. For each strain, the minimum inhibitory concentration (MIC) was determined using agar dilution test. Tests for enterobacteria producing extended spectrumβ-lactamases (ESBL) were performed using agar diffusion. Descriptive statistics was used for data analysis. Two of the isolated strains were submitted to the susceptibility test in biofilm. Of the collected samples, 8% presented Enterobacteria (mean of 6,141 CFU/mL) and no Pseudomona species was isolated. Positive samples were from NB in accommodation set or in the NB nursery. Enterobacter was the most prevalent genus and some strains were resistant to ampicillin, gentamicin and cephalothin. No ESBL strain was detected. Microorganisms in biofilms were resistant to all antibiotics, with concentrations four times higher than MIC. The presence of enterobacteria in the oral cavity of newborns, especially some strains resistant to normally used antibiotics, warns to the need for care to avoid the early colonization of this niche and the occurrence of a possible hospital infection in this age group.


Resumo O objetivo foi isolar enterobactérias e Pseudomonas da cavidade oral de recém-nascidos hospitalizados (RN) e determinar a prevalência e o perfil de sensibilidade aos antibióticos mais comumente utilizados para este grupo etário. Foram coletadas amostras da cavidade oral de NB com idade de 24-48 horas, usando swab. As amostras foram inoculadas em ágar MacConkey, incubadas e, as colônias contadas e identificadas. Para cada cepa, a concentração inibitória mínima (CIM) foi determinada utilizando teste de ágar diluição. Testes para enterobactérias produtoras de b-lactamases de espectro estendido (ESBL) foram realizados utilizando difusão em ágar. Estatística descritiva foi utilizada para análise dos resultados. Duas das cepas isoladas foram submetidas ao teste de susceptibilidade em biofilme. Das amostras coletadas, 8% apresentaram enterobactérias (média de 6,141 UFC / ml) e nenhuma espécie de Pseudomonas foi isolada. As amostras positivas foram de RN de alojamento conjunto ou RN de berçário. Enterobacter foi o gênero mais prevalente e algumas cepas foram resistentes à ampicilina gentamicina e cefalotina. Não foi detectada cepa ESBL. Micro-organismos em biofilme foram resistentes a todos os antibióticos, em concentrações quatro vezes superiores ao MIC. A presença de enterobactérias em cavidade oral de recém-nascidos, especialmente algumas cepas resistentes aos antibióticos normalmente utilizados, alerta para a necessidade de cuidados, evitando a colonização precoce deste nicho e a ocorrência de possível infecção nosocomial neste grupo etário.


Subject(s)
Humans , Infant, Newborn , Anti-Bacterial Agents/pharmacology , Enterobacteriaceae/drug effects , Mouth/microbiology , Pseudomonas/drug effects , Biofilms , Colony Count, Microbial , Drug Resistance, Bacterial , Enterobacteriaceae/isolation & purification , Microbial Sensitivity Tests , Pseudomonas/isolation & purification
13.
Arq. gastroenterol ; 54(3): 217-221, July-Sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-888207

ABSTRACT

ABSTRACT BACKGROUND: The clinical outcome of Helicobacter pylori infection has been associated with virulence factors. The presence of these factors is useful as molecular markers in the identification of the high risk for developing severe gastric pathologies. OBJECTIVE: To correlate the presence of virulence markers cagA and bab2A of H. pylori in oral and gastric biopsy samples. METHODS: An observational, prospective, descriptive, and cross-sectional study was carried out between September 2011 and September 2012. Patients suffering dyspepsia with indication for upper gastrointestinal video endoscopy who attended the Gastroenterology Service of the Hospital Dr. Julio C. Perrando were included. Epidemiological investigation was completed. To detect the bacteria and their virulence genes, samples of saliva, dental plaque and gastric biopsy were taken and processed by PCR. RESULTS: Sixty-one patients were selected for this study (30 women and 31 men). H. pylori was detected in 31 gastric biopsies and 31 oral samples. Significant difference between oral and gastric samples was found in cagA genotype. Agreement between oral and gastric genotypes was found in 38.7% of samples from the same patient. CONCLUSION: This study is the first in provide information about the genotypes of the Argentinean Northeast H. pylori strains. Despite the high prevalence of H. pylori infection, the most of patients had less virulent genotypes in oral cavity and gastric tissue. The cagA / babA2 combination was not frequent in the samples studied. There was not a statistical correlation between the virulence genes and gastroduodenal or oral diseases. Although in some patients the same genotype was found both in oral and gastric samples, it cannot be ensure that they corresponding to the same strain because a DNA sequencing was not performed.


RESUMO CONTEXTO: O resultado clínico da infecção por Helicobacter pylori tem sido associado com fatores de virulência. A presença desses fatores como marcadores moleculares é útil na identificação do risco elevado para o desenvolvimento de graves patologias gástricas. OBJETIVOS: Correlacionar a presença de marcadores de virulência cagA e bab2A do H. pylori em amostras de biópsias gástricas e orais. MÉTODOS: Um estudo observacional, prospectivo, descritivo e transversal foi realizado entre setembro de 2011 e setembro de 2012. Foram incluídos pacientes com sintomas de dispepsia com indicação de endoscopia gastrointestinal que compareceram ao Serviço de Gastroenterologia do Hospital Dr. Julio C. Perrando . Investigação epidemiológica foi concluída. Para detectar a bactéria e seus genes de virulência, amostras de saliva, placa dentária e biópsia gástrica foram tomadas e processadas pelo PCR. RESULTADOS: Sessenta e um pacientes foram selecionados para este estudo (30 mulheres e 31 homens). H. pylori foi detectado em 31 biópsias gástricas e 31 amostras orais. Foi encontrada diferença significativa entre as amostras orais e gástricas no genótipo cagA . A ocorrência simultânea entre genótipos orais e gástricos do mesmo paciente foi encontrada em 38,7% das amostras. CONCLUSÃO: Este é o primeiro estudo a fornecer informações sobre os genótipos das cepas do H. pylori no Nordeste Argentino. Apesar da alta prevalência da infecção pelo H. pylori , a maioria dos pacientes tinha genótipos menos virulentos na cavidade oral e tecido gástrico. A combinação cagA / babA2 não foi frequente nas amostras estudadas. Não houve correlação estatística entre os genes de virulência e doenças gastroduodenais ou orais. Embora em alguns pacientes o mesmo genótipo tenha sido encontrado tanto nas amostras orais quanto gástricas, não se pode garantir que correspondam à mesma variação, pois um sequenciamento de DNA não foi realizado.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Aged , Aged, 80 and over , Young Adult , Bacterial Proteins/genetics , Helicobacter pylori/pathogenicity , Helicobacter Infections/microbiology , Adhesins, Bacterial/genetics , Gastric Mucosa/microbiology , Mouth/microbiology , Antigens, Bacterial/genetics , Biopsy , Biomarkers/analysis , Cross-Sectional Studies , Prospective Studies , Helicobacter pylori/isolation & purification , Virulence Factors/genetics , Genotype , Middle Aged
14.
Rev. cuba. estomatol ; 54(1): 84-99, ene.-mar. 2017.
Article in Spanish | LILACS | ID: biblio-844859

ABSTRACT

Introducción: la cavidad bucal está compuesta de muchas superficies, cada una de ellas recubierta por una gran cantidad de bacterias, formando la biopelícula bacteriana. Algunas de estas bacterias han sido implicadas en enfermedades bucales como la caries y la periodontitis, que están entre las infecciones bacterianas más comunes en los seres humanos. Objetivo: profundizar en el estudio de la microbiota de los ecosistemas de la cavidad bucal a partir de una revisión bibliográfica para mejorar la comprensión de las funciones de la microbiota oral. Métodos: se realizó una revisión bibliográfica de febrero a junio de 2016 sobre los principales microorganismos que forman parte de los diferentes ecosistemas de la cavidad bucal. Los criterios de inclusión en la búsqueda fueron: microbiota oral, flora normal de la cavidad bucal, microbioma oral, ecosistemas primarios y secundarios de la cavidad bucal, microorganismos comensales de la cavidad bucal. La revisión se realizó a través de los buscadores y plataformas HINARI, SciELO y MEDLINE. Se revisaron 49 revistas de impacto de la Web of Science relacionadas con el tema, el 91 por ciento de la bibliografía correspondía a publicaciones realizadas durante los últimos 5 años. Análisis e integración de la información: se realizó un análisis sobre la composición de la microbiota bucal de los diferentes ecosistemas de la cavidad bucal. Conclusiones: el conocimiento de la microbiota bucal es una herramienta valiosa para la identificación correcta de las bacterias que están involucradas en complejas biopelículas bucales y nos permite entender mejor la patología bucal , hacer un diagnóstico efectivo y conocer si los cambios que predisponen a la enfermedad ocurren primero en el huésped o, por el contrario, a nivel microbiano(AU)


Introduction: the oral cavity is composed of many surfaces, each covered by a large number of bacteria forming the bacterial biofilm. Some of these bacteria have been implicated in oral diseases such as caries and periodontitis, which are among the most common bacterial infections in humans. Objective: by conducting a bibliographic review about the microbiota of oral cavity ecosystems improve our knowledge about the functions of the oral microbiota. Methods: a bibliographic review was conducted from February to June 2016 about the main microorganisms involved in the various oral cavity ecosystems. The search was based on the following inclusion criteria: oral microbiota, normal flora of the oral cavity, oral microbiome, primary and secondary oral cavity ecosystems, commensal microorganisms of the oral cavity. The review was based on search engines and platforms HINARI, SciELO and MEDLINE, and included 49 high impact journals from the Web of Science in which the topic was dealt with. 91 percent of the literature were publications from the last five years. Data analysis and integration: an analysis was performed of the composition of the oral microbiota of the various ecosystems in the oral cavity. Conclusions: knowledge about the oral microbiota is a valuable tool to accurately identify the bacteria involved in complex oral biofilms, allowing us to better understand oral pathology, make effective diagnoses, and determine whether the changes leading to disease occur first in the host or on a microbial level(AU)


Subject(s)
Humans , Bacterial Infections/prevention & control , Ecosystem , Microbiota , Mouth/microbiology , Databases, Bibliographic , Dental Plaque/prevention & control , Review Literature as Topic
15.
Braz. j. infect. dis ; 21(1): 27-34, Jan.-Feb. 2017. tab, graf
Article in English | LILACS | ID: biblio-839179

ABSTRACT

Abstract Objectives: This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n = 9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also. Methods: The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays. Statistical analysis was performed on SPSS v. 17.0 software using Friedman test and Wilcoxon signed ranks test. These tests were used to assess inter-group difference (p < 0.05). Results: Our results revealed that LPE exhibited a significant antimicrobial and anti-biofilm activities against the tested strains. A synergistic effect of LPEs and drug susceptibility was observed with a 2–8-fold reduction. Conclusion: LPE may be considered to have resistance-modifying activity. A more detailed investigation is necessary to determine the active compound responsible for therapeutic and disinfectant modulation.


Subject(s)
Humans , Child , Staphylococcus aureus/drug effects , Biofilms/growth & development , Drug Resistance, Bacterial/drug effects , Anti-Bacterial Agents/pharmacology , Mouth/microbiology , Reference Values , Tetracycline/pharmacology , Benzalkonium Compounds/pharmacology , Microbial Sensitivity Tests , Chlorhexidine/pharmacology , Polymerase Chain Reaction , Reproducibility of Results , Statistics, Nonparametric , Microscopy, Atomic Force/methods , Biofilms/drug effects , Lactobacillus plantarum/chemistry , Anti-Infective Agents, Local/pharmacology
16.
Rev. Inst. Med. Trop. Säo Paulo ; 59: e29, 2017. tab, graf
Article in English | LILACS | ID: biblio-842770

ABSTRACT

ABSTRACT The majority of HIV-infected patients develop Candida spp-associated clinical oral lesions. Studies have shown that asymptomatic oral colonization of Candida spp may lead to oral lesions or become a source of disseminated infections. The aim of this study was to verify the effects of periodontal conditions on Candida spp prevalence and Candida spp carriage in the oral cavity of HIV-infected patients compared to non-infected patients. Twenty-five patients not infected with HIV and 48 HIV-infected patients were classified according to periodontal conditions as being periodontal healthy or with periodontal disease. Candida spp carriage and classification were performed in oral rinse samples. Viral load and CD4+ T lymphocyte (CD4+L) counts were performed in blood samples from HIV-infected patients. No differences in Candida spp prevalence related to HIV status or periodontal condition were detected. However, Candida spp carriage was increased in periodontally affected HIV-infected patients when compared to periodontally healthy HIV-infected patients (p= 0.04). Periodontally healthy HIV-infected patients presented Candida spp carriage in similar levels as healthy or periodontally affected non-HIV-infected patients. Candida spp carriage was correlated with CD4+L counting in HIV-infected patients. We concluded that periodontal disease is associated with increased Candida spp carriage in HIV-infected patients and may be a predisposing factor to clinical manifestations of candidiasis.


Subject(s)
Humans , Male , Female , Adult , Periodontal Diseases/microbiology , Candida/classification , Candidiasis, Oral/microbiology , AIDS-Related Opportunistic Infections/microbiology , Periodontal Diseases/epidemiology , Brazil/epidemiology , Candida/isolation & purification , Candidiasis, Oral/epidemiology , Carrier State , AIDS-Related Opportunistic Infections/epidemiology , CD4 Lymphocyte Count , Viral Load , Mouth/microbiology
17.
Braz. oral res. (Online) ; 31: e21, 2017. tab, graf
Article in English | LILACS | ID: biblio-839522

ABSTRACT

Abstract The aim of this randomized, single blinded clinical trial was to evaluate the effect of a pre-procedural mouthwash containing cetylpyridinium chloride (CPC), zinc lactate (Zn) and sodium fluoride (F) in the reduction of viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler. Sixty systemically healthy volunteers receiving dental prophylaxis were randomly assigned to one of the following experimental groups (15 per group): (i) rinsing with 0.075% CPC, 0.28% Zn and 0.05% F (CPC+Zn+F), (ii) water or (iii) 0.12% chlorhexidine digluconate (CHX), and (iv) no rinsing. Viable bacteria were collected from different locations in the dental office on enriched TSA plates and anaerobically incubated for 72 hours. The colonies were counted and species were then identified by Checkerboard DNA–DNA Hybridization. The total number of colony-forming units (CFUs) detected in the aerosols from volunteers who rinsed with CPC+Zn+F or CHX was statistically significantly (p<0.05) lower than of those subjects who did not rinse or who rinsed with water. When all locations were considered together, the aerosols from the CPC+Zn+F and CHX groups showed, respectively, 70% and 77% fewer CFUs than those from the No Rinsing group and 61% and 70% than those from the Water group. The mean proportions of bacterial species from the orange complex were statistically significantly (p<0.05) lower in aerosols from the CPC+Zn+F and CHX groups compared with the others two groups. In conclusion, the mouthwash containing CPC+Zn+F, is effective in reducing viable bacteria in oral aerosol after a dental prophylaxis with ultrasonic scaler.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Young Adult , Aerosols , Anti-Infective Agents, Local/therapeutic use , Bacteria/drug effects , Mouthwashes/therapeutic use , Mouth/microbiology , Cetylpyridinium/therapeutic use , Colony Count, Microbial , DNA Probes , DNA, Bacterial , Lactates/therapeutic use , Mouthwashes/chemistry , Reproducibility of Results , Single-Blind Method , Sodium Fluoride/therapeutic use , Statistics, Nonparametric , Time Factors , Treatment Outcome , Zinc/therapeutic use
18.
Braz. oral res. (Online) ; 31: e38, 2017. tab
Article in English | LILACS | ID: biblio-839532

ABSTRACT

Abstract The aim of this study was to detect possible associations between respiratory pathogens from tracheal aspirate and oral biofilm samples in intubated patients in an intensive care unit (ICU), and to identify the most common respiratory pathogens in oral biofilm, particularly in patients that developed ventilator-associated pneumonia (VAP). Two oral biofilm samples were collected from the tongue of intubated patients (at admission and after 48 hours) and analyzed by culture with the Antibiotic Sensitivity Test. The results from the tongue biofilm samples were compared with the tracheal secretions samples. A total of 59.37% of patients exhibited the same species of pathogens in their tracheal aspirate and oral biofilm, of which 8 (42.1%) developed VAP, 10 (52.63%) did not develop pneumonia and one (5.26%) had aspiration pneumonia. There was a statistically significant association between presence of microorganisms in the tracheal and mouth samples for the following pathogens: Klebsiella pneumoniae, Candida albicans, Pseudomonas aeruginosa, Enterobacter gergoviae, Streptococcus spp and Serratia marcescens (p < 0.05). Pathogens that are present in tracheal aspirates of intubated patients can be detected in their oral cavity, especially in those who developed VAP or aspiration pneumonia. Thus, the results indicate that an improved oral care in these patients could decrease ICU pneumonia rates.


Subject(s)
Humans , Male , Female , Middle Aged , Bacteria/isolation & purification , Biofilms , Mouth/microbiology , Pneumonia, Ventilator-Associated/microbiology , Trachea/microbiology , Ventilators, Mechanical/microbiology , Candida albicans/isolation & purification , Dental Plaque/microbiology , Equipment Contamination , Intensive Care Units , Intubation, Intratracheal/adverse effects , Longitudinal Studies , Microbial Sensitivity Tests , Pneumonia, Aspiration/microbiology , Respiration, Artificial/adverse effects , Time Factors
19.
Int. j. odontostomatol. (Print) ; 10(2): 283-286, ago. 2016. ilus
Article in English | LILACS | ID: lil-794489

ABSTRACT

The objective of this study was to evaluate the prevalence of Candida spp. in complete dentures of institucionalized elderly, aged 60 or more, in a city of northeast of Brazil. A survey was conducted of the health profile and quantification of Candida spp. from isolation in Sabouraud agar. Our results showed that from 181 institutionalized elderlies, only 17 (66-84 years) met the inclusion criteria. 47.1 % were totally dependent, and 58.8 % needed help with hygiene. The most commonly used drugs were antihypertensive. The results showed a high prevalence of Candida spp. (64.5 %) in the dentures of institutionalized elderly and this may be a reflection of poor oral hygiene.


El objetivo fue asociar el uso de prótesis dentales totales y la prevalencia de Candida spp. en ancianos institucionalizados con 60 o más años de edad, en una ciudad del Nordeste de Brasil. Se llevó a cabo un estudio del perfil de salud y cuantificación de Candida spp. por aislamiento con agar Sabouraud. A partir de 181 ancianos institucionalizados, sólo 17 (66­84 años) cumplieron los criterios de inclusión. 47,1 % eran totalmente dependientes y 58,8 % necesitó ayuda con la higiene. Los fármacos más utilizados fueron antihipertensivos. Los resultados mostraron una alta prevalencia de Candida spp. (64,5 %) en las prótesis dentales totales de los ancianos institucionalizados y esto tal vez sea un reflejo de la deficiente higiene oral.


Subject(s)
Humans , Male , Female , Middle Aged , Aged , Aged, 80 and over , Staphylococcus aureus/isolation & purification , Streptococcus mutans/isolation & purification , Candida/isolation & purification , Denture, Complete, Upper/microbiology , Colony Count, Microbial , Prevalence , Cross-Sectional Studies , Ethics Committees , Mouth/microbiology
20.
J. appl. oral sci ; 24(3): 229-238, tab
Article in English | LILACS, BBO | ID: lil-787542

ABSTRACT

ABSTRACT Objectives This cross-sectional study compared the frequency of oral periodontopathogens and H. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery. Material and Methods One hundred and fifty-four men and women aged 18-65 were conveniently distributed into four groups. Two groups were composed of individuals who underwent bariatric surgery with (BP) (n=40) and without (BNP) (n=39) periodontitis and two obese control groups with (CP) (n=35) and without (CNP) (n=40) periodontitis. The oral pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Parvimonas micra, Treponema denticola, Tannerella forsythia, Campylobacter rectus, and Helicobacter pylori were detected by a polymerase chain reaction technique using saliva, tongue and stomach biopsy samples. Results Statistical analysis demonstrated that periodontopathogens were highly frequent in the mouth (up to 91.4%). In the bariatric surgically treated group, orally, P. gingivalis, T. denticola and T. forsythia were more frequent in periodontitis, while C. rectus was more frequent in non-periodontitis subjects. Stomach biopsies also revealed the high frequency of five oral species in both candidates for bariatric surgery (91.6%) and the bariatric (83.3%) groups. H. pylori was frequently detected in the mouth (50.0%) and stomach (83.3%). In the stomach, oral species and H. pylori appeared in lower frequency in the bariatric group. Conclusions Obese individuals showed high frequencies of periodontopathogens and H. pylori in their mouths and stomachs. Bariatric surgery showed an inverse microbial effect on oral and stomach environments by revealing higher oral and lower stomach bacterial frequencies.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Young Adult , Stomach/microbiology , Bacteria/isolation & purification , Helicobacter pylori/isolation & purification , Bariatric Surgery , Mouth/microbiology , Obesity/microbiology , Periodontitis/microbiology , Reference Values , Saliva/microbiology , Biopsy , Body Mass Index , Periodontal Index , Polymerase Chain Reaction , Cross-Sectional Studies , Analysis of Variance , Statistics, Nonparametric , Dental Plaque/microbiology , Obesity/surgery
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