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Braz. j. med. biol. res ; 53(11): e9974, 2020. graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1132490


Moyamoya disease (MMD) is currently thought to involve endothelial progenitor cells (EPCs). We investigated whether superparamagnetic iron oxide (SPIO) can be used to label EPCs. Mononuclear cells from 10 moyamoya disease patients were isolated, and cluster of differentiation 133 (CD133) positive cells sorted by magnetic-activated cell sorting were cultured in vitro. The positive rates of CD133, vascular endothelial growth factor receptor (VEGFR)-2, and cluster of differentiation 34 (CD34) were detected by flow cytometry. The cells were co-cultured with fluorescence labeled Dil-acetylated-low-density lipoprotein (Dil-ac-LDL) and Ulex europaeus agglutinin-1 (UEA-1) to observe the endocytosis of Dil-ac-LDL and binding to UEA-1. Prussian blue staining and transmission electron microscopy were used to observe the endocytosis of different SPIO concentrations in EPCs, and CCK-8 was used to detect proliferation of cells transfected with different concentrations of SPIO. T2 weighted imaging (T2WI) signals from magnetic resonance imaging after SPIO endocytosis were compared. Positive rates of CD133, VEGFR-2, and CD34 on sorted mononuclear cells were 68.2±3.8, 57.5±4.2, and 36.8±6.5%, respectively. The double-positive expression rate of CD34 and VEGFR-2 was 19.6±4.7%, and 83.1±10.4% of cells, which showed the uptake of Dil-ac-LDL and binding with UEA-1. The labeling efficiencies of SPIO at concentrations of 25 and 50 μg/mL were higher than for 12.5 μg/mL. The proliferation of cells was not influenced by SPIO concentrations of 12.5 and 25 μg/mL. After labeling, the T2WI of EPCs was reduced. The concentration of 25 μg/mL SPIO had high labeling efficiency detected by magnetic resonance imaging (MRI) without decreased EPCs viability.

Humans , Male , Adult , Middle Aged , Magnetite Nanoparticles , Endothelial Progenitor Cells , Moyamoya Disease/diagnostic imaging , Magnetic Resonance Imaging , Ferric Compounds , Cells, Cultured , Vascular Endothelial Growth Factor A , Metal Nanoparticles
Rev. bras. cir. cardiovasc ; 33(3): 309-311, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-958415


Abstract Moyamoya disease is a rare, idiopathic, progressive, occlusive disease of the internal carotid artery characterized by the development of collateral vasculature in the brain base. In patients with accompanying coronary artery disease, cardiopulmonary bypass posses a potential risk for perioperative cerebral ischemic complication. Herein, we report a 53-year-old male case of Moyamoya disease and coronary artery disease who was treated with off-pump coronary artery bypass grafting.

Humans , Male , Middle Aged , Coronary Stenosis/complications , Coronary Artery Bypass, Off-Pump/methods , Moyamoya Disease/surgery , Angiography, Digital Subtraction/methods , Risk Factors , Treatment Outcome , Coronary Angiography/methods , Ultrasonography, Doppler/methods , Coronary Stenosis/diagnostic imaging , Moyamoya Disease/diagnostic imaging
An. bras. dermatol ; 92(6): 870-873, Nov.-Dec. 2017. graf
Article in English | LILACS | ID: biblio-887126


Abstract: Neurofibromatosis type 1 is a multisystem genetic disease of autosomal dominant transmission that reveals important cutaneous manifestations such as café-au-lait spots, multiple neurofibromas, and ephelides in skin fold areas, as well as hamartomatous lesions in the eyes, bones, glands, and central nervous system. Moyamoya disease is a rare progressive vaso-occlusive disorder that occurs with important ischemic cerebrovascular events. Despite the rarity of this association in childhood, children diagnosed with neurofibromatosis type 1 and focal neurologic symptoms should be investigated for moyamoya syndrome. The present study reports the case of a pediatric patient with a rapidly progressive cerebrovascular accident and a late diagnosis of Neurofibromatosis type 1 associated with moyamoya disease.

Humans , Male , Child, Preschool , Neurofibromatosis 1/complications , Moyamoya Disease/complications , Tomography, X-Ray Computed , Neurofibromatosis 1/pathology , Neurofibromatosis 1/diagnostic imaging , Magnetic Resonance Angiography , Cafe-au-Lait Spots/pathology , Moyamoya Disease/pathology , Moyamoya Disease/diagnostic imaging