Your browser doesn't support javascript.
Show: 20 | 50 | 100
Results 1 - 5 de 5
Arq. neuropsiquiatr ; 70(8): 574-577, Aug. 2012. ilus, tab
Article in English | LILACS | ID: lil-645366


OBJECTIVE: To determine if the presence of oligoclonal bands (OB) at early stages of multiple sclerosis was associated with higher brain atrophy, when compared with patients without OB. METHODS: Relapsing-remitting multiple sclerosis (RRMS) patients with less than two years of disease onset and OB detection in cerebrospinal fluid (CSF) were included. SIENAX was used for total brain volume (TBV), gray matter volume (GMV), and white matter volume (WMV). RESULTS: Forty patients were included, 29 had positive IgG-OB. No differences were found between positive and negative patients in gender, expanded disability status scale (EDSS), treatment received, and T2/T1 lesion load. TBV in positive IgG-OB patients was 1.5 mm³ x 10(6) compared with 1.64 mm³ x 10(6) in the negative ones (p=0.02). GMV was 0.51 mm³ x 10(6) in positive IgG-OB compared with 0.62 mm³ x 10(6) in negative ones (p=0.002). No differences in WMV (p=0.09) were seen. CONCLUSIONS: IgG-OB in the CSF was related to neurodegeneration magnetic resonance (MR) markers in early RRMS.

OBJETIVO: Evaluar si la presencia de bandas oligoclonales (BO) en líquido cefalorraquídeo (LCR) de pacientes con esclerosis múltiple recaídaremisión (EMRR) se asociaba con mayor atrofia cerebral al inicio de la enfermedad. MÉTODOS: Pacientes con EMRR con menos que dos años del inicio de la enfermedad y en quiénes se realizó la búsqueda de IgG-BO en LCR fueron incluidos. SIENAX fue usado para la medición del volumen cerebral total (VCT), volumen de substancia gris (VSG) y volumen de sustancia blanca (VSB). RESULTADOS: Cuarenta pacientes fueron incluidos, 29 tenían IgG-BO positivo. No fueron encontradas diferencias entre pacientes positivos y negativos en: género, expanded disability status scale (EDSS), tratamiento recibido y carga lesional en resonancia magnética. El VCT en pacientes IgG-BO positivos fue de 1,5 mm³ x 10(6) versus 1,64 mm³ x 10(6) en BO negativo (p=0,02). El VSG fue 0,51 mm³ x 10(6) BO positivo versus 0,62 mm³ x 10(6) BO negativo (p=0,002). No fueron encontradas diferencias en VSB (p=0,09). CONCLUSIONES: La presencia de IgG-BO en el LCR se asoció con signos de neurodegeneración temprana en este estudio.

Adult , Female , Humans , Male , Brain Diseases/cerebrospinal fluid , Brain/pathology , Multiple Sclerosis, Relapsing-Remitting/cerebrospinal fluid , Neurodegenerative Diseases/cerebrospinal fluid , Oligoclonal Bands/cerebrospinal fluid , Atrophy/cerebrospinal fluid , Atrophy/pathology , Biomarkers/cerebrospinal fluid , Brain Diseases/pathology , Cross-Sectional Studies , Diagnosis, Differential , Disability Evaluation , Disease Progression , Magnetic Resonance Imaging , Multiple Sclerosis, Relapsing-Remitting/pathology , Neurodegenerative Diseases/pathology
Braz. j. med. biol. res ; 39(4): 441-445, Apr. 2006. ilus
Article in English | LILACS | ID: lil-425080


Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the human central nervous system. Although its etiology is unknown, the accumulation and activation of mononuclear cells in the central nervous system are crucial to its pathogenesis. Chemokines have been proposed to play a major role in the recruitment and activation of leukocytes in inflammatory sites. They are divided into subfamilies on the basis of the location of conserved cysteine residues. We determined the levels of some CC and CXC chemokines in the cerebrospinal fluid (CSF) of 23 relapsing-remitting MS patients under interferon-ß-1a therapy and 16 control subjects using ELISA. MS patients were categorized as having active or stable disease. CXCL10 was significantly increased in the CSF of active MS patients (mean ± SEM, 369.5 ± 69.3 pg/mL) when compared with controls (178.5 ± 29.1 pg/mL, P < 0.05). CSF levels of CCL2 were significantly lower in active MS (144.7 ± 14.4 pg/mL) than in controls (237.1 ± 16.4 pg/mL, P < 0.01). There was no difference in the concentration of CCL2 and CXCL10 between patients with stable MS and controls. CCL5 was not detectable in the CSF of most patients or controls. The qualitative and quantitative differences of chemokines in CSF during relapses of MS suggest that they may be useful as a marker of disease activity and of the mechanisms involved in the pathogenesis of the disease.

Adult , Female , Humans , Male , Middle Aged , Chemokines, CC/cerebrospinal fluid , Chemokines, CXC/cerebrospinal fluid , Multiple Sclerosis, Relapsing-Remitting/cerebrospinal fluid , Adjuvants, Immunologic/therapeutic use , Biomarkers/cerebrospinal fluid , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Interferon-beta/therapeutic use , Multiple Sclerosis, Relapsing-Remitting/drug therapy
Arq. neuropsiquiatr ; 63(4): 914-919, dez. 2005. tab, graf
Article in English | LILACS | ID: lil-418995


Os níveis de citocinas e síntese intratecal de IgG foram dosados no líquido cefalorraquidiano (LCR) e soro, com o objetivo de avaliar a atividade inflamatória em pacientes com esclerose múltipla durante remissão clínica. Foram detectados níveis elevados de citocinas pró-inflamatórias (TNFa e IFNg) no LCR e soro, sem alterações significativas na produção de IL12 e IL10. O perfil de produção das citocinas pró-inflamatórias estava associado ao aumento de leucócitos no LCR, assim como a presença de bandas oligoclonais IgG sugerindo síntese intratecal de IgG. Estes resultados sugerem que mesmo quando a doença está clinicamente silenciosa, a atividade inflamatória está presente nestes pacientes.

Adolescent , Adult , Child , Female , Humans , Male , Middle Aged , Cytokines/biosynthesis , Immunoglobulin G/biosynthesis , Multiple Sclerosis, Relapsing-Remitting/immunology , Oligoclonal Bands/biosynthesis , Biomarkers/blood , Biomarkers/cerebrospinal fluid , Case-Control Studies , Cytokines/blood , Cytokines/cerebrospinal fluid , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G/blood , Immunoglobulin G/cerebrospinal fluid , Inflammation/blood , Inflammation/cerebrospinal fluid , Inflammation/immunology , Leukocyte Count , Multiple Sclerosis, Relapsing-Remitting/blood , Multiple Sclerosis, Relapsing-Remitting/cerebrospinal fluid , Oligoclonal Bands/blood , Oligoclonal Bands/cerebrospinal fluid
Arq. neuropsiquiatr ; 59(1): 18-22, Mar. 2001. tab
Article in English | LILACS | ID: lil-284231


Cytokines and adhesion molecules have been implicated in the pathogenesis of multiple sclerosis (MS), a chronic inflammatory disease of the central nervous system. In this study we analyzed intrathecal (CSF) and serum levels of soluble intercellular adhesion molecule (ICAM-1) and TNFalphaR (60kD) from 20 patients with clinically definite MS during acute relapse or stable disease. Comparing to control groups of healthy individuals and patients with intervertebral herniated disc, MS patients showed increased levels (p< 0.001) of sICAM-1 and TNFalphaR in both serum and CSF samples. Regardless stage of disease there was no significant difference in the levels of sICAM-1 during acute relapse (657 + or - 124.9 ng/ml) or remission (627 + or - 36.2 ng/ml). A steady increase of TNFalphaR (60kD) in both serum and CSF, indicate the existence of a continuous inflammatory process within the brain tissue of MS patients despite absence of clinical signs of disease activity

Humans , Male , Female , Adolescent , Adult , Middle Aged , Intercellular Adhesion Molecule-1/cerebrospinal fluid , Multiple Sclerosis, Relapsing-Remitting/physiopathology , Receptors, Tumor Necrosis Factor/analysis , Tumor Necrosis Factor-alpha/cerebrospinal fluid , Case-Control Studies , Enzyme-Linked Immunosorbent Assay , Intercellular Adhesion Molecule-1/blood , Multiple Sclerosis, Relapsing-Remitting/blood , Multiple Sclerosis, Relapsing-Remitting/cerebrospinal fluid , Receptors, Tumor Necrosis Factor/blood