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1.
Biocell ; 37(1): 1-9, Apr. 2013. ilus, graf
Article in English | LILACS | ID: lil-694715

ABSTRACT

Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.


Subject(s)
Animals , Mice , Apoptosis/drug effects , Hydrogen Peroxide/pharmacology , Mitochondria/pathology , Myoblasts, Skeletal/pathology , Oxidants/pharmacology , Blotting, Western , Cell Culture Techniques , Cells, Cultured , /metabolism , Cell Division/drug effects , Immunoprecipitation , Microscopy, Fluorescence , Membrane Potential, Mitochondrial/drug effects , Mitochondria/drug effects , Mitochondria/metabolism , Myoblasts, Skeletal/drug effects , Myoblasts, Skeletal/metabolism , /metabolism
2.
Article in English | WPRIM | ID: wpr-32320

ABSTRACT

Recombinant human epidermal growth factor (rhEGF) stimulates the proliferation and migration of epithelial cells in human cell culture systems and animal models of partial-thickness skin wounds. This study investigated the effect of a topical rhEGF ointment on the rate of wound healing and skin re-epithelialization in a rat full thickness wound model, and verified whether or not the rhEGF treatment affected both myofibroblast proliferation and collagen synthesis in the dermis. When rhEGF (10 microgram/g ointment) was applied topically twice a day for 14 days, there was significantly enhanced wound closure from the 5th to the 12th day compared with the control (ointment base treatment) group. A histological examination at the postoperative 7th day revealed that the rhEGF treatment increased the number of proliferating nuclear antigen immunoreactive cells in the epidermis layer. In addition, the immunoreactive area of alpha-smooth muscle actin and the expression of prolyl 4-hydroxylase were significantly higher than those of the control group. Overall, a topical treatment of rhEGF ointment promotes wound healing by increasing the rate of epidermal proliferation and accelerating the level of wound contraction related to myofibroblast proliferation and collagen deposition.


Subject(s)
Actins/genetics , Administration, Topical , Animals , Cell Proliferation/drug effects , Collagen/biosynthesis , Epidermal Growth Factor , Gene Expression Regulation , Male , Myoblasts, Skeletal/drug effects , Proliferating Cell Nuclear Antigen/genetics , Rats , Rats, Sprague-Dawley , Wound Healing/drug effects
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