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1.
Article in Chinese | WPRIM | ID: wpr-879185

ABSTRACT

Rhus chinensis is an important resource plant. The aqueous extract of R. chinensis roots or stems was to produce Shuguantong Syrup, which is mainly used for the treatment of coronary heart disease and angina pectoris with definite curative effect. On this basis, the crude phenolic part of R. chinensis prepared by macroporous resin was evaluated for the cardio protective effect against myocardial ischemia in mice. The results showed that the phenolic part group with oral administration at the dosages of 190.8-381.6 mg·kg~(-1), compared with the model group, reduced the values of left ventricular end systolic diameter(LVEDs) and the left ventricular end diastolic diameter(LVEDd), and increased the cardiac ejection fraction(EF) and left ventricular fractional shortening(FS) rate, which could effectively improve cardiac function and exert its anti-myocardial ischemia effect, and reduce the rising levels of creatine kinase isoenzyme(CK-MB) and lactate dehydrogenase(LDH) in serum. HE staining showed that the phenolic part group reduced the infiltration of myocardial inflammatory cells and alleviated the degree of myocardial fibrosis and collagen deposition. TUNEL staining showed that the blue-green fluorescence of the phenolic part group decreased successively, and the degree of myocardial cell apoptosis was reduced. Immunohistochemical staining suggested that it could reduce the number of positive cells for p53 protein expression and significantly improve myocardial cell damage. All above data suggested that the phenolic part group had an anti-mycardial ischemis effect. Related mechanism studies revealed that the crude phenolic part could regulate the expressions of the p53 gene(p53), Bcl-2-associated X protein(Bax), B lymphoma-2 gene(Bcl-2), and caspase-3 protein(caspase-3) in myocardial tissue, suggesting that it could reduce cardiac remodeling and myocardial ischemic damage, and improve cardiac function by inhibiting myocardial apoptosis.This research laid a foundation for the elucidation of the pharmacological ingredients R. chinensis.


Subject(s)
Animals , Apoptosis , Mice , Myocardial Ischemia/drug therapy , Myocardium , Myocytes, Cardiac , Plant Extracts/pharmacology , Rhus , bcl-2-Associated X Protein
2.
Article in Chinese | WPRIM | ID: wpr-879814

ABSTRACT

This article summarizes and analyzes the clinical features and gene mutation characteristics of children with noncompaction of the ventricular myocardium (NVM). For the 6 children with NVM (4 boys and 2 girls), the age of onset ranged from 3 months to 12 years. Of the 6 children, 5 had arrhythmia, 3 had cardiac insufficiency, 1 had poor mental state, and 1 had chest distress and sighing. NVM-related gene mutations were detected in 4 children, among whom 2 had


Subject(s)
Cardiomyopathies , Child , Child, Preschool , Echocardiography , Female , Heart Ventricles/diagnostic imaging , Humans , Infant , Male , Mutation , Myocardium
3.
ABC., imagem cardiovasc ; 34(3)2021. tab, graf
Article in Portuguese | LILACS | ID: biblio-1292072

ABSTRACT

Fundamentos: O papel da cintilografia de perfusão miocárdica em pacientes assintomáticos permanece restrito a situações clínicas muito específicas, muitas delas abordadas nos Critérios de Uso Apropriado (AUC) de Cintilografia de Perfusão Miocárdica. Objetivo: Realizar uma análise crítica da aplicação desses critérios nas indicações de exames realizados em pacientes assintomáticos do Instituto Dante Pazzanese de Cardiologia, cuja população é notadamente de alto risco cardiovascular. Métodos: Foram selecionados pacientes assintomáticos que realizaram cintilografia de perfusão miocárdica para pesquisa de isquemia. As indicações dos exames foram classificadas em apropriadas, inapropriadas ou incertas. Hipocaptação fixa, hipocaptação transitória ou dilatação isquêmica transitória foram consideradas exames alterados. Na análise estatística, buscou-se avaliar a correlação entre o grau de recomendação das indicações e a presença de exames alterados. Resultados: A partir de uma seleção inicial de 2.999 prontuários, 490 foram considerados assintomáticos e incluídos conforme critérios de inclusão estabelecidos previamente. Apenas 9,8% das indicações foram inapropriadas, enquanto que 61,4% foram apropriadas, e 28,8% foram incertas. A hipocaptação fixa do radiofármaco ocorreu em 43,5% dos casos e a hipocaptação transitória, em 16,1%. Solicitar o exame de maneira apropriada ou incerta foi fator preditor de exame com resultado alterado nesta população. Conclusão: O uso dos critérios de uso apropriado da cintilografia de perfusão miocárdica mostrou-se eficaz em predizer exames alterados em uma população assintomática de alto risco cardiovascular, especialmente no grupo de pacientes com indicação incerta, o que pode significar que algumas das indicações consideradas incertas talvez sejam apropriadas para uma população de alto risco cardiovascular. (AU)


Subject(s)
Humans , Male , Female , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Myocardial Ischemia/diagnostic imaging , Myocardial Perfusion Imaging/methods , Asymptomatic Diseases , Heart Disease Risk Factors , Fibrosis , Cross-Sectional Studies , Multivariate Analysis , Retrospective Studies , Myocardium/pathology
4.
ABC., imagem cardiovasc ; 34(3)2021. tab, graf
Article in Portuguese | LILACS | ID: biblio-1292025

ABSTRACT

Fundamento: A identificação precoce do dano miocárdico parece ser importante na abordagem do paciente com doença de Chagas. A ecocardiografia com strain obtida por speckle tracking e a avaliação da fibrose miocárdica por meio da ressonância magnética cardíaca podem ser métodos diagnósticos promissores nesse sentido. Objetivo: Avaliar o acometimento miocárdico especificamente na forma crônica cardíaca leve da doença de Chagas por meio do strain por speckle tracking e da fibrose miocárdica pela ressonância magnética cardíaca, além de suas correlações. Método: Estudo de corte transversal que analisou portadores da forma cardíaca crônica leve da doença de Chagas (fração de ejeção preservada) submetidos à ecocardiografia com strain por speckle tracking e à ressonância magnética cardíaca. Resultados: Foram incluídos 21 participantes (mulheres: 62%; idade: 54 ± 5 anos). A prevalência de fibrose miocárdica por meio do realce tardio miocárdico foi de 50%. O strain longitudinal global encontrava-se diminuído em 17 pacientes (81%), com mediana de 14,1% (intervalo interquartil de 12,1 a 16,3). Os valores do mapa T1 encontravam-se, em média, elevados nos portadores de doença de Chagas (993 ± 163 ms). O mapa T1 foi significativamente correlacionado com o strain longitudinal global (r= 0,634; p = 0,015). Além disso, o índice de dispersão mecânica, obtido por strain, estava aumentado (> 55 ms) em 84%, com a maior área sob a curva Característica de Operação do Receptor (área sob a curva de 0,696; intervalo de confiança de 95% de 0,412-0,981) para discriminação de fibrose pelo realce tardio miocárdico. Conclusão: O strain miocárdico e o mapa T1 se comportam como marcadores precoces do dano miocárdico na cardiopatia chagásica crônica leve. O índice de dispersão mecânica estava elevado e foi o parâmetro que melhor se correlacionou com o realce tardio miocárdico. (AU)


Background: The early identification of myocardial damage seems important in the management of patients with Chagas disease. However, it is unknown whether speckle tracking echocardiography strain and cardiac magnetic resonance imaging are promising methods for assessing myocardial involvement and fibrosis, respectively. Objective: To evaluate myocardial involvement in the mild chronic cardiac form of Chagas disease using speckle tracking strain and myocardial fibrosis by cardiac magnetic resonance and assess their correlations. Method: This cross-sectional study analyzed patients with the mild chronic cardiac form of Chagas disease (preserved ejection fraction) using speckle tracking echocardiography and cardiac magnetic resonance. Results: The study included 21 participants (women: 62%; age: 54 ± 5 years). The prevalence of myocardial fibrosis was 50% on delayed myocardial enhancement. The median global longitudinal strain was decreased (14.1%; interquartile range, 12.1­16.3%) in 17 patients (81%). The mean T1 mapping value was high in patients with Chagas disease (993 ± 163 ms). The T1 map was significantly correlated with the global longitudinal strain (r = 0.634; p = 0.015). In addition, the mechanical dispersion index obtained by strain was increased (>55 ms) by 84%, with the largest area under the receiver operating characteristic curve (area under the curve, 0.696; 95% confidence interval, 0.412­0.981) for fibrosis discrimination by delayed myocardial enhancement. Conclusion: Myocardial strain and T1 mapping are early markers of myocardial damage in mild chronic Chagas heart disease. The mechanical dispersion index was high and the most closely correlated with delayed myocardial enhancement. (AU)


Subject(s)
Humans , Male , Female , Middle Aged , Chagas Cardiomyopathy/physiopathology , Chagas Cardiomyopathy/diagnostic imaging , Heart/diagnostic imaging , Myocardium/pathology , Prognosis , Stroke Volume , Fibrosis , Magnetic Resonance Imaging , Echocardiography/methods , Linear Models , Chronic Disease , Cross-Sectional Studies , ROC Curve
5.
REME rev. min. enferm ; 25: e1354, 2021. tab
Article in Portuguese | LILACS, BDENF | ID: biblio-1287714

ABSTRACT

RESUMO Introdução: observando a diferença de perfil dos pacientes submetidos à cirurgia de revascularização do miocárdio daqueles submetidos à correção cirúrgica de valvopatias, considera-se importante a avaliação do estado emocional de acordo com a cirurgia a ser realizada. Objetivo: comparar os sintomas de ansiedade, depressão e ansiedade cardíaca de pacientes no pré-operatório, segundo o tipo de cirurgia cardíaca a ser realizada: revascularização do miocárdio ou cirurgia de correção de valvopatias. Método: estudo observacional, analítico, de corte transversal, realizado nas enfermarias de cirurgia de um hospital universitário do interior paulista. Uma amostra consecutiva e não probabilística foi constituída pelos pacientes maiores de idade que vieram de casa para a internação e cujo agendamento para a realização da cirurgia foi eletivo. Para a avaliação dos sintomas de ansiedade e depressão, foi utilizado o instrumento Hospital Anxiety and Depression Scale; já para a avaliação da ansiedade cardíaca, foi utilizado o "Questionário de Ansiedade Cardíaca". Para a comparação das medidas dos sintomas, segundo o tipo de cirurgia, foi realizado o teste de Mann-Whitney para amostras independentes, com nível de significância de 5%. Resultados: os grupos foram homogêneos entre si, quanto à caracterização sociodemográfica, exceto para idade. Pacientes em pré-operatório de correção de valvopatias apresentaram escore maior para os sintomas de ansiedade cardíaca quando comparados com pacientes em pré-operatório de revascularização do miocárdio, e a diferença encontrada foi estatisticamente significativa (p=0,020). Conclusão: pacientes em pré-operatório de correção de valvopatias apresentaram mais sintomas de ansiedade cardíaca quando comparados com pacientes em pré-operatório de revascularização do miocárdio.


RESUMEN Introducción: al observar la diferencia en el perfil de los pacientes sometidos a cirugía de revascularización miocárdica respecto a los sometidos a corrección quirúrgica de valvulopatías, se considera importante evaluar el estado emocional de acuerdo con la cirugía a realizar. Objetivo: comparar los síntomas de ansiedad, depresión y ansiedad cardíaca de los pacientes en el período preoperatorio, según el tipo de cirugía cardíaca a realizar: revascularización miocárdica o cirugía de corrección de valvulopatías. Método: estudio observacional, analítico, transversal, realizado en las salas de recuperación de cirugía de un hospital universitario del interior de São Paulo. Una muestra consecutiva y no probabilística estuvo conformada por pacientes mayores que acudieron a su domicilio para ser hospitalizados y cuya cita para la cirugía fue electiva. Para evaluar los síntomas de ansiedad y depresión se utilizó el instrumento Escala Hospitalaria de Ansiedad y Depresión; para la evaluación de la ansiedad cardíaca se utilizó el "Cuestionario de Ansiedad Cardíaca". Para comparar las medidas de síntomas, según el tipo de cirugía, se realizó la prueba de Mann-Whitney para muestras independientes, con un nivel de significancia del 5%. Resultados: los grupos fueron homogéneos entre sí, encuanto a caracterización sociodemográfica, excepto por edad. Los pacientes en el período preoperatorio para la corrección de valvulopatías tuvieron una puntuación más alta para los síntomas de ansiedad cardíaca en comparación con los pacientes en el período preoperatorio de revascularización miocárdica, y la diferencia encontrada fue estadísticamente significativa (p=0,020). Conclusión: los pacientes en período preoperatorio de corrección de valvulopatías presentaron más síntomas de ansiedad cardíaca en comparación con los pacientes en el período preoperatorio de revascularización miocárdica.


ABSTRACT Introduction: observing the difference in profile of patients undergoing coronary artery bypass graft surgery from those undergoing surgical correction of valvular heart disease, it is considered important to assess the emotional state according to the surgery to be performed. Objective: to compare the symptoms of anxiety, depression, and cardiac anxiety of patients in the preoperative period, according to the type of cardiac surgery to be performed: myocardial revascularization or surgery to correct valvular heart diseases. Method: observational, analytical, cross-sectional study carried out in the surgery wards of a university hospital in the countryside of São Paulo. A consecutive and non-probabilistic sample was made up of older patients who came home for hospitalization and whose appointment for the surgery was elective. To evaluate the symptoms of anxiety and depression, the Hospital Anxiety and Depression Scale instrument was used; for the assessment of cardiac anxiety, the "Heart Anxiety Questionnaire" was used. To compare the measures of symptoms, according to the type of surgery, the Mann-Whitney test was performed for independent samples, with a significance level of 5%. Results: the groups were homogeneous among themselves, in terms of sociodemographic characterization, except for age. Patients in the preoperative period for correction of valvular heart disease had a higher score for the symptoms of cardiac anxiety when compared with patients in the preoperative period of myocardial revascularization, and the difference found was statistically significant (p=0.020). Conclusion: patients in the preoperative period for correction of valvular heart disease presented more symptoms of cardiac anxiety when compared with patients in the preoperative period of myocardial revascularization.


Subject(s)
Humans , Anxiety , Cardiac Surgical Procedures , Myocardial Revascularization , Cardiovascular Nursing , Myocardium
6.
Clinics ; 76: e1958, 2021. tab, graf
Article in English | LILACS | ID: biblio-1153932

ABSTRACT

OBJECTIVES: To evaluate the effects of sympathectomy on the myocardium in an experimental model. METHODS: The study evaluated three groups of male Wistar rats: control (CT; n=15), left unilateral sympathectomy (UNI; n=15), and bilateral sympathectomy (BIL; n=31). Sympathectomy was performed by injection of absolute alcohol into the space of the spinous process of the C7 vertebra. After 6 weeks, we assessed the chronotropic properties at rest and stress, cardiovascular autonomic modulation, myocardial and peripheral catecholamines, and beta-adrenergic receptors in the myocardium. The treadmill test consisted of an escalated protocol with a velocity increment until the maximal velocity tolerated by the animal was reached. RESULTS: The bilateral group had higher levels of peripheral catecholamines, and consequently, a higher heart rate (HR) and blood pressure levels. This suggests that the activation of a compensatory pathway in this group may have deleterious effects. The BIL group had basal tachycardia immediately before the exercise test and increased tachycardia at peak exercise (p<0.01); the blood pressure had the same pattern (p=0.0365). The variables related to autonomic modulation were not significantly different between groups, with the exception of the high frequency (HF) variable, which showed significant differences in CT vs UNI. There was no significant difference in beta receptor expression between groups. There was a higher concentration of peripheral norepinephrine in the BIL group (p=0.0001), and no significant difference in myocardial norepinephrine (p=0.09). CONCLUSION: These findings suggest that an extra cardiac compensatory pathway increases the sympathetic tonus and maintains a higher HR and higher levels of peripheral catecholamines in the procedure groups. The increase in HF activity can be interpreted as an attempt to increase the parasympathetic tonus to balance the greater sympathetic activity.


Subject(s)
Animals , Male , Rats , Sympathectomy , Myocardium , Blood Pressure , Rats, Wistar , Heart Rate
7.
Acta méd. colomb ; 45(4): 59-62, Oct.-Dec. 2020. graf
Article in English | LILACS, COLNAL | ID: biblio-1278143

ABSTRACT

Abstract Cocaine abuse is related to a greater risk of cardiovascular events such as myocardial infarction and ischemic cerebrovascular accidents. The pathophysiological mechanisms are not fully understood, although the formation of intravascular thrombi and accelerated atherosclerosis are notable findings. We report the case of a 38-year-old man addicted to cocaine who presented ischemic events in the form of acute myocardial infarction complicated by heart failure. The pathophysiology of cocaine-induced vascular damage and the treatment of complications are discussed.


Resumen El abuso de cocaína se asocia con un mayor riesgo de eventos cardiovasculares, como infarto de miocardio y accidente cerebrovascular isquémico. Los mecanismos fisiopatológicos no se entienden completamente, aunque la formación de trombos intravasculares y la aterosclerosis acelerada son hallazgos destacados. Reportamos el caso de un hombre de 38 años adicto a la cocaína, que presentó eventos isquémicos caracterizados por infarto agudo de miocardio complicado por insuficiencia cardíaca. Se discute la fisiopatología del daño vascular inducido por la cocaína y el manejo de las complicaciones.


Subject(s)
Humans , Male , Adult , Myocardial Infarction , Endothelium, Vascular , Cocaine , Stroke , Myocardium
8.
Rev. bras. cir. cardiovasc ; 35(5): 824-830, Sept.-Oct. 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1137319

ABSTRACT

Abstract Objective: To investigate the cardiovascular effects produced by transthoracic application of low-intensity pulsed ultrasound therapy (LIPUST). Methods: Three-month-old male Wistar rats (± 300 g, N=16) were randomly allocated in two groups, namely SHAM (control group, faked procedures) and UST (animals treated with LIPUST). These animals, under anesthesia, were instrumented (femoral artery and vein catheterization) for hemodynamic recordings (mean blood pressure [MBP], heart rate [HR]) and blood biochemical profile (lipids, creatine kinase-myocardial band [CK-MB]). Then, LIPUST (spatial average-temporal average [ISATA] 1-MHz, power 0.1 to 1.2 W/cm2, pulsed 2:8 ms, cycle at 30%, for three minutes) was applied to animals from the UST group, externally to their thorax. SHAM animals were equally manipulated, but without application of ultrasound energy. After the hemodynamic and biochemical measurements, animals were sacrificed, and their hearts were mounted in a Langendorff apparatus for coronary reactivity evaluation. Standard histology techniques were employed to analyze the hearts. Results: LIPUST application caused statistically significant reductions in MBP (92±4 vs. 106±1 mmHg) and HR (345±14 vs. 380±17 rpm) when compared with SHAM procedures. UST rats exhibited higher CK-MB levels (318±55 vs. 198±26 U/dL) and lower plasma triglycerides levels (38±7 vs. 70±10 mg/dL) than SHAM animals. Coronary reactivity was not significantly changed by LIPUST. Cardiac histopathology showed an increase in capillary permeability in treated animals when compared with SHAM animals. Conclusion: Noninvasive LIPUST induces significant metabolic and hemodynamic changes, including intensity-dependent bradycardia and hypotension, indicating a possible therapeutic effect for cardiac events.


Subject(s)
Animals , Male , Rats , Bradycardia/therapy , Hypotension , Myocardium , Rats, Wistar , Ultrasonic Waves , Heart , Hemodynamics
10.
Rev. bras. cir. cardiovasc ; 35(4): 530-538, July-Aug. 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1137302

ABSTRACT

Abstract In December 2019, a striking appearance of new cases of viral pneumonia in Wuhan led to the detection of a novel coronavirus (SARS-CoV2). By analyzing patients with severe manifestations, it became apparent that 20 to 35% of patients who died had preexisting cardiovascular disease. This finding warrants the important need to discuss the influence of SARS-CoV2 infection on the cardiovascular system and hemodynamics in the context of clinical management, particularly during mechanical ventilation. The SARS-CoV2 enters human cells through the spike protein binding to angiotensin-converting enzyme 2 (ACE2), which is important to cardiovascular modulation and endothelial signaling. As ACE2 is highly expressed in lung tissue, patients have been progressing to acute respiratory injury at an alarming frequency during the Coronavirus Disease (COVID-19) pandemic. Moreover, COVID-19 leads to high D-dimer levels and prothrombin time, which indicates a substantial coagulation disorder. It seems that an overwhelming inflammatory and thrombogenic condition is responsible for a mismatching of ventilation and perfusion, with a somewhat near-normal static lung compliance, which describes two types of pulmonary conditions. As such, positive pressure during invasive mechanical ventilation (IMV) must be applied with caution. The authors of this review appeal to the necessity of paying closer attention to assess microhemodynamic repercussion, by monitoring central venous oxygen saturation during strategies of IMV. It is well known that a severe respiratory infection and a scattered inflammatory process can cause non-ischemic myocardial injury, including progression to myocarditis. Early strategies that guide clinical decisions can be lifesaving and prevent extended myocardial damage. Moreover, cardiopulmonary failure refractory to standard treatment may necessitate the use of extreme therapeutic strategies, such as extracorporeal membrane oxygenation.


Subject(s)
Humans , Pneumonia, Viral/complications , Cardiovascular System/virology , Coronavirus Infections/complications , Hemodynamics , Respiration, Artificial , Cardiovascular System/physiopathology , Coronavirus Infections , Pandemics , Betacoronavirus , Myocardium/pathology
14.
Arq. bras. cardiol ; 114(1): 100-105, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1055084

ABSTRACT

Abstract Background: The emergence of coronary heart disease is increased with menopause, physical inactivity and with dyslipidemia. Physical training is known to promote the improvement of cardiovascular functions. Objective: To investigate the effects of aerobic physical training on the left ventricle in ovariectomized LDL knockout mice. Methods: Thirty animals were divided into 6 groups (n = 5): Sedentary non-ovariectomized control; Sedentary ovariectomized control; Trained ovariectomized control; Sedentary non-ovariectomized LDL-knockout, sedentary ovariectomized LDL-knockout and trained ovariectomized LDL-knockout. We analyzed the average parameters of apparent density of collagen fibers types I and III, and metalloproteinase type 2 and type 9, were considered significant p < 0.05. Results: The results showed that the proposed exercise protocol altered the volume of type I collagen fibers, altered collagen remodeling parameters (MMP-2), and also reduced the 8-hydroxy-2'-deoxyguanosine (8OHdG) oxidative stress parameter. Conclusion: Moderate intensity aerobic training acts on collagen fiber volume, on collagen remodeling with the reduction of oxidative stress in the left ventricles of ovariectomized LDL-knockout mice.


Resumo Fundamento: O surgimento da doença cardíaca coronariana aumenta com a menopausa, inatividade física e dislipidemia. Sabe-se que o treinamento físico promove a melhora das funções cardiovasculares Objectivo: Investigar os efeitos do treinamento físico aeróbico sobre o ventrículo esquerdo em camundongos LDL knockout ovariectomizadas. Métodos: Trinta animais foram divididos em 6 grupos (n = 5): controle sedentário não ovariectomizado, controle sedentário ovariectomizado, controle treinado ovariectomizado, sedentário LDL-knockout não ovariectomizado, sedentário LDL-knockout ovariectomizado e treinado LDL-knockout ovariectomizado. Analisamos os parâmetros médios da densidade de volume de fibras colágenas tipo I e III, e metaloproteinases 2 e 9. Valores de p < 0,05 foram considerados significativos. Resultados: Os resultados mostram que o protocolo de exercício proposto alterou o volume de fibras colágenas do tipo I e os parâmetros de remodelamento do colágeno (MMP-2), e ainda reduziu o parâmetro de estresse oxidativo do 8-hidroxi-2'-deoxiganosina (8-OhdG). Conclusão: O treinamento aeróbico de intensidade moderada age sobre o volume das fibras colágenas e sobre o remodelamento de colágeno, com redução do estresse oxidativo em ventrículos esquerdos de camundongos ovariectomizados LDLr Knockout.


Subject(s)
Animals , Female , Rats , Matrix Metalloproteinase 2/metabolism , Matrix Metalloproteinase 9/metabolism , Collagen Type I/metabolism , Collagen Type III/metabolism , Inflammation/physiopathology , Myocardium/metabolism , Physical Conditioning, Animal/physiology , Immunohistochemistry , Ovariectomy , Mice, Knockout , Oxidative Stress/physiology , Models, Animal
15.
Article in English | WPRIM | ID: wpr-812991

ABSTRACT

OBJECTIVES@#To study myocardial perfusion and systolic function in patients with different coronary artery stenosis by myocardial contrast echocardiography (MCE) and two-dimensional speckle tracking imaging (2D-STI).@*METHODS@#MCE, 2D-STI and coronary angiography were conducted in patients with coronary heart disease in our hospital. Myocardial segments were divided into 4 groups according to the degree of coronary artery stenosis: group A (normal group), group B (mild stenosis, 50%-75%), group C (moderate stenosis, 76%-90%) and group D (severe stenosis, 91%-100%). Blood volume, blood flow velocity, blood flow and longitudinal myocardial strain value (SL) in each group were measured by analysis software for MCE and 2D-STI. The differences in blood volume, blood flow velocity, blood flow and SL were evaluated.@*RESULTS@#The blood flow velocity and blood flow in B, C and D groups were decreased gradually, with significant difference (0.05). There were significant differences in SL among the 4 groups (<0.05).@*CONCLUSIONS@#There is a good correlation between coronary stenosis degree and myocardial perfusion or systolic function. The ability of 2D-STI to detect mild stenosis of coronary artery is better than MCE.


Subject(s)
Coronary Circulation , Coronary Stenosis , Echocardiography , Humans , Myocardium , Systole
16.
Article in Chinese | WPRIM | ID: wpr-826680

ABSTRACT

OBJECTIVE@#To observe the effects of electroacupuncture (EA) pretreatment on the cardiac ejection fraction (EF), the number of macrophages in spleen and heart, and the expression of nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) and interleukin-1β (IL-1β) in myocardium in mice with acute myocardial ischemia, and to explore the possible mechanism of EA pretreatment on promoting myocardial protection.@*METHODS@#A total of 30 male C57BL/6J mice were randomly divided into a control group, a model group and an EA pretreatment group, 10 rats in each group. The acute myocardial ischemia model was established by ligating the left anterior descending branch of the coronary artery in the model group and EA pretreatment group, while threading but no ligating at left anterior descending branch of the coronary artery was applied in the control group. In the EA pretreatment group, mice were intervented with EA at bilateral "Neiguan" (PC 6), disperse-dense wave, frequency of 2 Hz/15 Hz, intensity of 2 mA; each EA treatment last for 20 min, once a day, and 3-day treatment was given before model establishment. The EF value was evaluated by ultrasonic cardiogram; the number of macrophages in spleen and heart was measured by flow cytometry; the expression level of NLRP3 and IL-1β in myocardium was measured by Western blot.@*RESULTS@#Compared with the control group, the EF value was decreased in the model group (<0.001), the number of macrophages in the heart and spleen was increased (<0.001), and the expression level of NLRP3 and IL-1β in the myocardium was increased (<0.001, <0.01). Compared with the model group, the EF value was increased in the EA pretreatment group (<0.01), the number of macrophages in the heart and spleen was decreased (<0.01), and the expression level of NLRP3 and IL-1β in the myocardium was decreased (<0.01, <0.05).@*CONCLUSION@#EA pretreatment could reduce the number of macrophages in spleen and heart, down-regulate the expression of NLRP3 and IL-1β in myocardial tissue in mice with acute myocardial ischemia, which could relieve the local inflammatory response and achieve the myocardial protective effect.


Subject(s)
Acupuncture Points , Animals , Electroacupuncture , Heart , Physiology , Inflammation , Allergy and Immunology , Interleukin-1beta , Metabolism , Macrophages , Cell Biology , Male , Mice , Mice, Inbred C57BL , Myocardial Ischemia , Allergy and Immunology , Therapeutics , Myocardium , NLR Family, Pyrin Domain-Containing 3 Protein , Metabolism , Random Allocation , Spleen
17.
Article in English | WPRIM | ID: wpr-811138

ABSTRACT

BACKGROUND: Recent studies have shown that microRNAs (miRNAs) are involved in the process of cardiomyocyte apoptosis. We have previously reported that granulocyte-colony stimulating factor (G-CSF) ameliorated diastolic dysfunction and attenuated cardiomyocyte apoptosis in a rat model of diabetic cardiomyopathy. In this study, we hypothesized a regulatory role of cardiac miRNAs in the mechanism of the anti-apoptotic effect of G-CSF in a diabetic cardiomyopathy rat model.METHODS: Rats were given a high-fat diet and low-dose streptozotocin injection and then randomly allocated to receive treatment with either G-CSF or saline. H9c2 rat cardiomyocytes were cultured under a high glucose (HG) condition to induce diabetic cardiomyopathy in vitro. We examined the extent of apoptosis, miRNA expression, and miRNA target genes in the myocardium and H9c2 cells.RESULTS: G-CSF treatment significantly decreased apoptosis and reduced miR-34a expression in diabetic myocardium and H9c2 cells under the HG condition. G-CSF treatment also significantly increased B-cell lymphoma 2 (Bcl-2) protein expression as a target for miR-34a. In addition, transfection with an miR-34a mimic significantly increased apoptosis and decreased Bcl-2 luciferase activity in H9c2 cells.CONCLUSION: Our results indicate that G-CSF might have an anti-apoptotic effect through down-regulation of miR-34a in a diabetic cardiomyopathy rat model.


Subject(s)
Animals , Apoptosis , Diabetic Cardiomyopathies , Diet, High-Fat , Down-Regulation , Glucose , Granulocyte Colony-Stimulating Factor , In Vitro Techniques , Luciferases , Lymphoma, B-Cell , MicroRNAs , Models, Animal , Myocardium , Myocytes, Cardiac , Rats , Streptozocin , Transfection
18.
Korean Circulation Journal ; : 379-394, 2020.
Article in English | WPRIM | ID: wpr-816679

ABSTRACT

It is now recognized that the heart can behave as a true endocrine organ, which can modulate the function of other tissues. Emerging evidence has shown that visceral fat is one such distant organ the heart communicates with. In fact, it appears that bi-directional crosstalk between adipose tissue and the myocardium is crucial to maintenance of normal function in both organs. In particular, factors secreted from the heart are now known to influence the metabolic activity of adipose tissue and other organs, as well as modulate the release of metabolic substrates and signaling molecules from the periphery. This review summarizes current knowledge regarding primary cardiokines and adipokines involved in heart-fat crosstalk, as well as implications of their dysregulation for cardiovascular health.


Subject(s)
Adipocytes , Adipokines , Adipose Tissue , Heart , Intra-Abdominal Fat , Myocardium , Myocytes, Cardiac
19.
Korean Circulation Journal ; : 420-429, 2020.
Article in English | WPRIM | ID: wpr-816675

ABSTRACT

BACKGROUND AND OBJECTIVES: It is difficult to estimate the depth of the focal source by activation mapping. The present study was performed to demonstrate the usefulness of a simple maneuver in estimating the depth of the focal activation source (S).METHODS: A total of 44 sites (15 shallow, depth<3.5 mm; 29 deep, depth>5.5 mm) were analyzed in 3 canine left ventricles under general anesthesia. A custom-made bipolar needle electrode was used to simulate a focal activation source. A mapping catheter with an electrode tip size of 2 mm, band electrode size of 1 mm, and inter-electrode spacing of 2-10-2 mm was placed at the mapping area. The position of the center of the distal 2 electrodes was kept at the insertion site of the needle electrode. The time interval between distal and proximal electrodes of the mapping catheter (T(tachy)) was measured during needle electrode pacing. The time interval between distal and proximal electrodes (T(pace)) was measured during pacing with distal electrodes of a mapping catheter. Depth index (δ) was defined as T(tachy)/T(pace). Using in vivo data, simulation was performed to evaluate the depth and δ.RESULTS: Ttachy was 24±7.7 ms and 15±5.6 ms for shallow source and deep source simulation, respectively (p<0.001). δ values were 0.86±0.21 and 0.55±0.21 for shallow source and deep source simulation, respectively (p<0.001). According to simulation data, if δ<0.52, the depth of the focal source will be >5.5 mm.CONCLUSIONS: T(tachy) was shorter and δ was smaller for a deep S than for a shallow S.


Subject(s)
Anesthesia, General , Catheters , Electrodes , Heart Ventricles , Myocardium , Needles , Tachycardia, Ventricular
20.
Korean Circulation Journal ; : 430-431, 2020.
Article in English | WPRIM | ID: wpr-816674

ABSTRACT

No abstract available.


Subject(s)
Myocardium
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