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1.
Braz. j. biol ; 84: e252364, 2024. graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1355885

ABSTRACT

Abstract Understanding morphological and physiological changes under different light conditions in native fruit species in juveniles' stage is important, as it indicate the appropriate environment to achieve vigorous saplings. We aimed to verify growth and morphophysiological changes under shade gradient in feijoa (Acca sellowiana (O. Berg) Burret) to achieve good quality saplings adequate to improve cultivation in orchards. The saplings were grown for twenty-one-month under four shading treatments (0%, 30%, 50%, and 80%). Growth, photosynthetic pigments, gas exchanges, chlorophyll fluorescence, and leaf anatomy parameters were evaluated. Saplings under full sun and 30% shade had higher height and diameter growth and dry mass accumulation due to higher photosynthesis rate. As main acclimatization mechanisms in feijoa saplings under 80% shade were developed larger leaf area, reduced leaf blade thickness, and enhanced quantum yield of photosystem II. Even so, the net CO2 assimilation and the electron transport rate was lower and, consequently, there was a restriction on the growth and dry mass in saplings under deep shade. Therefore, to obtain higher quality feijoa saplings, we recommend that it be carried out in full sun or up to 30% shade, to maximize the sapling vigor in nurseries and, later, this light environment can also be used in orchards for favor growth and fruit production.


Resumo A verificação de mudanças morfológicas e fisiológicas sob diferentes condições luminosas em espécies frutíferas nativas em estágio juvenil é importante, uma vez que indicam o ambiente adequado para a formação de mudas com alto vigor. Objetivou-se verificar o crescimento e as alterações morfofisiológicas sob gradiente de sombreamento em mudas de feijoa (Acca sellowiana (O. Berg) Burret) para obter mudas de boa qualidade, adequadas para fomentar os plantios da espécie em pomares. As mudas foram cultivadas por vinte e um meses sob quatro tratamentos de sombreamento (0%, 30%, 50% e 80%). Foram avaliados parâmetros de crescimento, pigmentos fotossintéticos, trocas gasosas, fluorescência da clorofila e anatomia foliar. Mudas a pleno sol e 30% de sombra apresentaram maior crescimento em altura, diâmetro e acúmulo de massa seca, devido à maior taxa de fotossíntese. Como principais mecanismos de aclimatação sob 80% de sombra, as mudas desenvolveram maior área foliar, redução da espessura do limbo foliar e aumento do rendimento quântico do fotossistema II. Mesmo assim, a assimilação líquida de CO2 e a taxa de transporte de elétrons foram menores e, consequentemente, houve restrição ao crescimento e acúmulo de massa seca das mudas no maior nível de sombreamento. Portanto, para a obtenção de mudas de feijoa de maior qualidade, recomendamos que seja realizada a pleno sol ou até 30% de sombra, para maximizar o vigor das mudas em viveiros e, posteriormente, este ambiente de luz também pode ser utilizado em pomares para favorecer o crescimento e a produção de frutos.


Subject(s)
Myrtaceae , Feijoa , Photosynthesis , Plant Leaves , Acclimatization , Light
2.
Braz. j. biol ; 81(3): 728-736, July-Sept. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153401

ABSTRACT

Abstract Xylella fastidiosa is a plant-pathogenic bacterium that lives inside host xylem vessels, where it forms biofilm which is believed to be responsible for disrupting the passage of water and nutrients. Pectobacterium carotovorum is a Gram-negative plant-specific bacterium that causes not only soft rot in various plant hosts, but also blackleg in potato by plant cell wall degradation. Chagas disease, which is caused by Trypanosoma cruzi, has been commonly treated with nifurtimox and benzonidazole, two drugs that cause several side effects. As a result, the use of natural products for treating bacterial and neglected diseases has increased in recent years and plants have become a promising alternative to developing new medicines. Therefore, this study aimed to determine, for the first time, the chemical composition of essential oil from Psidium guajava flowers (PG-EO) and to evaluate its in vitro anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi and cytotoxic activities. PG-EO was obtained by hydrodistillation in a Clevenger apparatus while its chemical composition was determined by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Major compounds identified in PG-EO were α-cadinol (37.8%), β-caryophyllene (12.2%), nerolidol (9.1%), α-selinene (8.8%), β-selinene (7.4%) and caryophyllene oxide (7.2%). Results showed that the PG-EO had strong trypanocidal activity against the trypomastigote forms of Trypanosoma cruzi (IC50 = 14.6 μg/mL), promising antibacterial activity against X. fastidiosa (MIC = 12.5 μg/mL) and P. carotovorum (MIC = 62.5 μg/mL), and moderate cytotoxicity against LLCMK2 adherent epithelial cells in the concentration range (CC50 = 250.5 μg/mL). In short, the PG-EO can be considered a new source of bioactive compounds for the development of pesticides and trypanocide drugs.


Resumo Xylella fastidiosa é uma bactéria patogênica que vive dentro dos vasos do xilema hospedeiro, onde forma um biofilme responsável por interromper a passagem de água e nutrientes. Pectobacterium carotovorum é uma bactéria Gram-negativa que causa não só podridão macia em várias plantas hospedeiras, mas também canela-preta na batata por degradação da parede celular da planta. A doença de Chagas, causada pelo Trypanosoma cruzi, é comumente tratada com nifurtimox e benzonidazol, duas drogas que causam vários efeitos colaterais. Como resultado, o uso de produtos naturais para o tratamento de doenças bacterianas e negligenciadas aumentou nos últimos anos e as plantas continuam sendo uma alternativa promissora para o desenvolvimento de novos medicamentos. Portanto, este estudo teve como objetivo determinar, pela primeira vez, a composição química do óleo essencial de flores de Psidium guajava (PG-EO) e avaliar suas propriedades anti-Xylella fastidiosa, anti-Pectobacterium carotovorum, anti-Trypanosoma cruzi e citotóxica in vitro. PG-EO foi obtido por hidrodestilação em um aparelho Clevenger, enquanto sua composição química foi determinada por cromatografia em fase gasosa com detecção por ionização por chama (CG-DIC) e por cromatografia em fase gasosa acoplada à espectrometria de massa (CG-EM). Os principais compostos identificados no PG-EO foram α-cadinol (37,8%), β-cariofileno (12,2%), nerolidol (9,1%), α-selineno (8,8%), β-selineno (7,4%) e óxido de cariofileno (7,2%). Os resultados mostraram que o PG-EO apresentou forte atividade tripanocida contra as formas tripomastigotas de T. cruzi (CI50 = 14,6 μg/mL), promissora atividade antibacteriana contra X. fastidiosa (MIC = 12,5 μg/mL) e P. carotovorum (MIC = 62,5 μg/mL) e citotoxicidade moderada contra células epiteliais aderentes (LLCMK2) na faixa de concentração (CC50 = 250,5 μg/mL). Em suma, o PG-EO pode ser considerado uma nova fonte de compostos bioativos para o desenvolvimento de pesticidas e drogas tripanocidas.


Subject(s)
Oils, Volatile/pharmacology , Myrtaceae , Psidium , Microbial Sensitivity Tests , Plant Leaves , Flowers , Xylella
3.
Braz. j. biol ; 81(3): 621-631, July-Sept. 2021. tab, graf
Article in English | LILACS | ID: biblio-1153391

ABSTRACT

Abstract Campomanesia xanthocarpa, a plant belonging to the Myrtaceae family, is popularly known as gabiroba. Leaves of gabiroba has been popularly used to treat various diseases, including inflammatory, renal, and digestive, among others. Additionally, studies have shown an effect to reduce blood cholesterol levels. The aim of this study was to evaluate the antihyperglycemic and hypolipidemic effects of Campomanesia xanthocarpa seed extract in hyperglycemic rats. The results showed that 400 mg/kg of seed extract was able to decrease blood glucose levels and to increase the muscular and hepatic glycogen content as well as to inhibit the sucrase and maltase activity. At doses of 200 mg/kg and 800 mg/kg, the activity of these enzymes was also reduced. In the lipid profile 400 mg/kg produced a decrease in total and LDL cholesterol serum levels; and with 200 mg/kg there was an increase in HDL cholesterol levels. The extract did not present hepatic and renal toxic effects at the different doses tested. The results suggest that the treatment with Campomanesia xanthocarpa seeds extract is useful in reducing glycemia, total cholesterol and LDL levels with potential adjuvant therapeutic in the treatment of diabetes and hypercholesterolemia, however, additional pharmacological and toxicological studies are still required.


Resumo Campomanesia xanthocarpa, planta pertencente à família Mirtaceae, é popularmente conhecida como gabiroba. Folhas da gabiroba são popularmente usadas para tratar de doenças inflamatórias, renais, digestivas entre outras. Além disso, estudos têm mostrado um efeito redutor dos níveis de colesterol. O objetivo deste estudo foi avaliar os efeitos anti-hiperglicêmico e hipolipidêmico do extrato de sementes de Campomanesia xanthocarpa em ratos hiperglicêmicos. Os resultados mostraram que 400 mg/kg do extrato da semente foi capaz de reduzir os níveis de glicose sanguínea e aumentar o conteúdo de glicogênio hepático e muscular, bem como inibir a atividade da maltase e sacarase. Na dose de 200 mg/kg e 800 mg/kg, a atividade das enzimas também foi reduzida. No perfil lipídico, 400 mg/kg produziu uma redução nos níveis séricos de colesterol total e LDL e com 200 mg/kg houve um aumento nos níveis de colesterol HDL. O extrato não apresentou efeitos tóxicos hepáticos e renais nas doses testadas. Os resultados sugerem que o tratamento com o extrato de Campomanesia xanthocarpa é eficaz na redução da glicemia, de colesterol total e LDL com potencial para tratamento adjuvante do diabetes e hipercolesterolemia, no entanto estudos farmacológicos e toxicológicos adicionais são necessários.


Subject(s)
Animals , Rats , Carbon Dioxide , Myrtaceae , Seeds , Plant Extracts/pharmacology , Hypoglycemic Agents/pharmacology
4.
Rev. cuba. med. trop ; 73(2): e607, 2021. tab, graf
Article in Spanish | LILACS, CUMED | ID: biblio-1347484

ABSTRACT

Introducción: Streptococcus mutans participa en el origen y progreso de la caries dental, una de las enfermedades más prevalentes a nivel mundial. Su control requiere métodos seguros y accesibles para la población. Myrciaria dubia (Kunth) McVaugh (Myrtaceae) (camu camu) es un árbol nativo de la amazonía peruana. La capacidad antimicrobiana de los componentes de su fruto ya se ha comprobado. Objetivo: Evaluar la actividad antibacteriana in vitro del extracto hidroetanólico de M. dubia contra S. mutans ATCC 35658. Métodos: Investigación experimental con posprueba y grupos controles. El extracto de la pulpa del fruto de M. dubia se obtuvo mediante maceración hidroetanólica. Las concentraciones evaluadas fueron 25 mg/mL, 50 mg/mL y 75 mg/mL. La capacidad antibacteriana se determinó mediante el método de difusión en disco. Gluconato de clorhexidina 0,12 por ciento fue el control positivo y el dimetilsulfóxido al 1 por ciento el control negativo. Resultados: La actividad antibacteriana se incrementó de manera directamente proporcional a la concentración del extracto. La concentración de 75 mg/mL mostró una media de inhibición de 18,2 ± 0,774 mm, seguido de la concentración de 50 mg/mL con una media de inhibición de 14,6 ± 1,055 mm y la concentración de 25 mg/mL con un halo de inhibición promedio de 10,1± 0,833 mm. La zona de inhibición del control positivo fue de 16,5 ± 0,516 mm. Existe diferencia estadísticamente significativa entre la concentración de 75 mg/mL y el control positivo (p< 0,05). Conclusiones: El extracto hidroetanólico de M. dubia muestra actividad antibacteriana in vitro de tipo bactericida sobre S. mutans ATCC 35668(AU)


Introduction: Streptococcus mutans is involved in the genesis and progress of dental caries, one of the most prevalent diseases worldwide, whose control requires safe methods accessible to the population. Myrciaria dubia (Kunth) McVaugh (Myrtaceae) (camu camu) is a tree native to the Peruvian Amazon. The antimicrobial capacity of the components of its fruit has already been verified. Objective: Evaluate the in vitro antibacterial activity of M. dubia hydroethanolic extract against S. mutans ATCC 35658. Methods: An experimental study was conducted with post-test analysis and control groups. The extract from the pulp of the fruit of M. dubia was obtained by hydroethanolic maceration. The concentrations evaluated were 25 mg/mL, 50 mg/mL and 75 mg/mL. Antibacterial capacity was determined by the disc diffusion method. The positive control was 0.12 percent chlorhexidine gluconate, whereas the negative control was 1 percent dimethyl sulfoxide. Results: The antibacterial activity increased directly proportional to the concentration of the extract. The concentration of 75 mg/mL showed a mean inhibition of 18.2 ± 0.774 mm, followed by the concentration of 50 mg/mL with a mean inhibition of 14.6 ± 1.055 mm and the concentration of 25 mg/mL with an average inhibition halo of 10.1 ± 0.833 mm. The zone of inhibition of the positive control was 16.5 ± 0.516 mm. There is a statistically significant difference between the concentration of 75 mg / mL and the positive control (p< 0.05). Conclusions: The M. dubia hydroethanolic extract displays in vitro antibacterial bactericidal activity against S. mutans ATCC 35668(AU)


Subject(s)
Humans , Male , Female , Streptococcus mutans , Myrtaceae , Dental Caries , Herpes Zoster , Anti-Bacterial Agents , In Vitro Techniques
5.
Acta amaz ; 51(2): 91-101, jun. 2021.
Article in English | LILACS | ID: biblio-1353303

ABSTRACT

Myrciaria dubia (Myrtaceae) é um arbusto que produz frutos com altas concentrações de ácido ascórbico (1380 a 1490 mg 100 g-1 na polpa e 2050 mg 100 g-1 na casca), 20 vezes mais que a acerola e 100 vezes mais que o limão. Nativo da região amazônica, ocorre naturalmente em ambientes sazonalmente inundados, nas margens de lagos e rios, mas também é cultivado comercialmente em terra firme. Neste estudo, observações fenológicas e avaliação da produtividade de frutos foram realizadas em uma população natural de camu-camu em ambiente inundável e em uma plantação em terra firme, na Amazônia central. Visitas quinzenais foram feitas durante um ano para coleta de dados sobre floração, frutificação e fenologia foliar. No auge da floração, os botões florais foram marcados e acompanhados até a maturação dos frutos, que foram colhidos para biometria e contagem das sementes. Os frutos variaram de 1,01 a 2,73 cm em diâmetro e 1,14 a 10,87 g em peso, com maior produção de frutos em ambiente natural. A fenologia diferiu entre os habitats e a sincronia das fenofases foi maior na população natural. Apesar de ser nativo de florestas inundáveis, a produção de frutos em terra firme ocorreu ao longo do ano, sugerindo que há poucas restrições para o desenvolvimento de frutos em cultivo.(AU)


Subject(s)
Myrtaceae , Flowers , Rainforest , Fruit
6.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 226-243, may. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1342815

ABSTRACT

Several species of the Myrcia genus have been used in folk medicine to treat diabetes. Therefore, the aim of this work was to investigate the inhibitory activity of α-glucosidase and pancreatic lipase in the crude extract (EBF) and in the ethyl acetate fraction (FFA) of Myrcia hatschbachii, as well as to identify isolated phenolic compounds and to evaluate the antioxidant property and preliminary in vitro toxicity against Artemia salina. EBF (IC50: 3.21 µg/mL) and FFA (IC50: 1.14 µg/mL) showed inhibitory activity superior to acarbose (IC50: 193.65 µg/mL). In addition, they showed inhibitory effects of pancreatic lipase (IC50: 556.58 µg/mL for EBF and 532.68 µg/mL for FFA), antioxidant potential, absence of preliminary toxicity and presence of gallic andellagic acids in FFA. The relevant results in the inhibition of α-glucosidase and pancreatic lipase motivate new studies for the development of herbal medicines that assist in the treatment of diabetic patients.


Varias especies del género Myrcia se han utilizado en la medicina popular para tratar la diabetes. Por lo tanto, el objetivo de este trabajo fue investigar la actividad inhibitoria de la α-glucosidasa y la lipasa pancreática en el extracto crudo (EBF) y en la fracción de acetato de etilo (FFA) de Myrcia hatschbachii, así como identificar compuestos fenólicos aislados y evaluar la propiedad antioxidante y toxicidad in vitro preliminar contra Artemia salina. EBF (IC50: 3.21 µg/mL) y FFA (IC50: 1.14 µg/mL) mostraron una actividad inhibitoria superior a la acarbosa (IC50: 193.65 µg/mL). Además, mostraron efectos inhibitorios de la lipasa pancreática (IC50: 556.58 µg/mL para EBF y 532.68 µg/mL para FFA), potencial antioxidante, ausencia de toxicidad preliminar y presencia de ácidos gálico y elágico en FFA. Los resultados relevantes en la inhibición de la α-glucosidasa y la lipasa pancreática motivan nuevos estudios para el desarrollo de medicamentos a base de hierbas que ayudan en el tratamiento de pacientes diabéticos.


Subject(s)
Plant Extracts/pharmacology , Myrtaceae/chemistry , Glycoside Hydrolase Inhibitors/pharmacology , Lipase/drug effects , Antioxidants/pharmacology , Pancreas/enzymology , Phenols/analysis , X-Ray Diffraction , In Vitro Techniques , Plant Extracts/toxicity , Plant Extracts/chemistry , Free Radical Scavengers , Complex Mixtures , Ellagic Acid , Gallic Acid , Antioxidants/chemistry
7.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 260-269, may. 2021. tab
Article in English | LILACS | ID: biblio-1342820

ABSTRACT

The aim of this study was to undertake a screening experiment on essential oils (EO) of Myrcia lundiana, Ocimum basilicum and Lippia alba against six food-spoiling pathogenic bacteria. Seventy-two (72) samples were initially analyzed fo antimicrobial activity based on the agar diffusion test. The minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined for the 12 samples which showed greatest antimicrobial potential in this stage. Two samples of L. alba, three samples of M. lundiana and seven samples of O. basilicum showed a MIC of 0.12-125 µL/mL for the six tested bacteria. Of these, the EO of O. basilicum cultivar Maria Bonita stood out with the lowest MIC and MBC. Thus, a mixture simulating this essential oil was prepared from commercial standards of the compounds (±)-linalool, geraniol and 1,8-cineole. Significantly higher MIC and MBC were detected in the simulation compared to the respective EO, suggesting a synergistic effect between compounds.


El objetivo de este estudio fue realizar un experimento de detección en aceites esenciales (AE) de Myrcia lundiana, Ocimum basilicum y Lippia alba contra seis bacterias patógenas que estropean los alimentos. Setenta y dos (72) muestras fueron analizadas inicialmente para la actividad antimicrobiana basada en la prueba de difusión en agar. Se determinaron las concentraciones mínimas inhibitoria (CMI) y bactericida (CMB) para las 12 muestras que mostraron el mayor potencial antimicrobiano en esta etapa. Dos muestras de L. alba, tres muestras de M. lundiana y siete muestras de O. basilicum mostraron un CMI de 0.12-125 µL/mL para las seis bacterias analizadas. De estos, el AE de O. basilicum cultivar Maria Bonita se destacó con el CMI y CMB más bajos. Por lo tanto, se preparó una mezcla que simula este aceite esencial a partir de los estándares comerciales de los compuestos de (±)-linalol, geraniol y 1,8-cineol. Se detectaron CMI y CMB significativamente más altos en la simulación en comparación con el AE respectivo, lo que sugiere un efecto sinérgico entre los compuestos.


Subject(s)
Bacteria/drug effects , Oils, Volatile/pharmacology , Ocimum basilicum/chemistry , Myrtaceae/chemistry , Lippia/chemistry , Anti-Bacterial Agents/pharmacology , Sesquiterpenes/analysis , Oils, Volatile/chemistry , Microbial Sensitivity Tests , Food , Anti-Bacterial Agents/chemistry
9.
Bol. latinoam. Caribe plantas med. aromát ; 20(2): 147-161, 2021. tab, graf
Article in English | MTYCI, LILACS, MTYCI | ID: biblio-1145965

ABSTRACT

Myrcianthes pungens (Myrtaceae) es un árbol nativo de Brasil conocido como guabijú. En nuestro estudio, describimos la composición química del aceite esencial de las hojas secas de M. pungens, la actividad antioxidante por diferentes métodos y la actividad antibacteriana contra Staphylococcus aureus, Bacillus cereus y Pseudomonas aeruginosa. La identificación química se realizó mediante un cromatógrafo de gases acoplado a un espectrómetro de masas y la actividad antibacteriana se evaluó mediante un método de microdilución en caldo. El aceite esencial de la hoja presentó sesquiterpenos de hidrocarburos (42,6%) como su principal clase química cuyos compuestos principales fueron ß-cariofileno (11,7%) y 1,8-cineol (10,1%). La mayor protección antioxidante fue de 57.5 a 63.3% de ß-caroteno. La mayor actividad antibacteriana fue contra S. aureus (MIC de 78.12 µg/mL). Este valor fue 2,56 veces menor que el control positivo de estreptomicina. M. pungens es una fuente prometedora de moléculas naturales con potencial estudio para el desarrollo de productos farmacológicos, cosméticos y alimenticios.


Myrcianthes pungens (Myrtaceae) is a native tree to Brazil known as guabijú. In our study, we described the chemical composition of the essential oil from M. pungens dried leaves, the antioxidant activity by different methods and antibacterial activity against Staphylococcus aureus, Bacillus cereus and Pseudomonas aeruginosa. The chemical identification was done by gas chromatograph coupled to mass spectrometer and antibacterial activity was evaluated by broth microdilution method. The leaf essential oil presented hydrocarbon sesquiterpenes (42.6%) as its main chemical class whose major compounds were ß-caryophyllene (11.7%) and 1,8-cineole (10.1%). The best antioxidant protection was from 57.5 to 63.3% of ß-carotene. The highest antibacterial activity was against S. aureus (MIC of 78.12 µg/mL). This value was 2.56-fold lower than the positive streptomycin control. M. pungens is a promising source of natural molecules with potential study for the development of pharmacological, cosmetic and food products.


Subject(s)
Myrtaceae , Anti-Bacterial Agents , Antioxidants , Plants, Medicinal , Pseudomonas aeruginosa , Staphylococcus aureus , Bacillus cereus , Brazil , Gas Chromatographers , Medicine, Traditional
10.
Rev. Soc. Bras. Med. Trop ; 54: e00922020, 2021. tab, graf
Article in English | ColecionaSUS, LILACS, ColecionaSUS, SES-SP | ID: biblio-1143892

ABSTRACT

Abstract INTRODUCTION: Despite their widespread usage, synthetic insecticides and larvicides are harmful for controlling disease-causing mosquitoes owing to the development of resistance. The leaves of Eugenia astringens, Myrrhinium atropurpureum, and Neomitranthes obscura were collected from Marambaia and Grumari restingas. The safety and larvicidal efficacy of their extracts were tested against Aedes (Stegomyia) aegypti L. and Simulium (Chirostilbia) pertinax Kollar. METHODS: The dry leaves were subjected to static maceration extraction using 90% methanol. A. aegypti and S. pertinax larvae were exposed to 7.5, 12.5, and 25.0 µL/mL of the extracts (n= 30). The larvicidal activity after 24 h and 48 h, and the mortality, were determined. The median lethal concentration (CL50) was estimated by a Finney's probit model. RESULTS: M. atropurpureum and E. astringens extracts exhibited the strongest larvicidal effects against A. aegypti. M. atropurpureum extracts (25 µL/mL) caused mortalities of over 50% and 100% after 24 h and 48 h, respectively (CL50 = 11.10 and 9.68 ppm, respectively). E. astringens extracts (25 µL/mL) caused mortalities of 50% and 63.33% after 24 h and 48 h, respectively. High concentrations of N. obscura extracts induced a maximum mortality of 46.66% in A. aegypti larvae after 48 h (CL50= 25 ppm). The larvae of S. pertinax showed 100% mortality following exposure to all the plant extracts at all the tested concentrations after 24 h. CONCLUSIONS: The extracts of M. atropurpuerum exhibited the strongest larvicidal activity against A. aegypti. The larvae of S. pertinax were sensitive to all the extracts at all the tested concentrations.


Subject(s)
Animals , Simuliidae , Aedes , Culex , Myrtaceae , Insecticides/pharmacology , Anopheles , Plant Extracts/pharmacology , Plant Leaves , Larva
11.
Bol. latinoam. Caribe plantas med. aromát ; 20(2): 132-146, 2021. ilus, tab
Article in English | LILACS | ID: biblio-1342208

ABSTRACT

We investigated the effects of dichloromethane extract (DME) from Myrcia splendenson alterations caused by type 2 diabetes in the blood and kidney of rats, in order to reduce side effects caused by synthetic drugs. Rats received streptozotocin (60 mg/kg),15 minutes after nicotinamide (120 mg/kg) or water. After 72 hours, the glycemic levels were evaluated to confirm diabetes and the animals received (15 days) DME (25, 50, 100 or 150 mg/Kg) or water. DME partially reversed hyperglycemia and (100 and 150 mg/kg) reversed hypertriglyceridemia. Histopathological findings elucidated that DME reduced damage to pancreatic islets. DME 150 mg/kgreversed the increases in TBA-RS, the reduction in the sulfhydryl content, 100 and 150 mg/kg increased CAT, reversed the decrease in GSH-Px and increased it activity in the blood. DME 150 mg/kg reversed CAT and GSH-Px reductions in the kidney. We believe that DME effects might be dependent on the presence of phenolic compounds.


Investigamos los efectos del extracto de diclorometano (DME)de Myrcia splendens sobre las alteraciones causadas por la diabetes tipo 2 en la sangre y los riñones de las ratas, para reducir los efectos secundarios causados por las drogas sintéticas. Las ratas recibieron estreptozotocina (60 mg/kg), 15 minutos después de la nicotinamida (120 mg/kg) o agua. Después de 72 horas, se confirmo la diabetes y los animales recibieron (15 días) DME (25, 50, 100 o 150 mg/Kg) o agua. DME revierte parcialmente la hiperglucemia y revierte la hipertrigliceridemia. DME redujo el daño a los islotes pancreáticos. DME revirtió los aumentos en TBA-RS, la reducción en el contenido de sulfhidrilo, aumentó la CAT, revirtió la disminución en GSH-Px y aumentó su actividad en la sangre. Además, DME revirtió las reducciones de CAT y GSH-Px en el riñón. Creemos que los efectos provocados por DME pueden depender de la presencia de compuestos fenólicos.


Subject(s)
Animals , Male , Rats , Plant Extracts/administration & dosage , Myrtaceae/chemistry , Diabetes Mellitus, Experimental/drug therapy , Hypoglycemic Agents/administration & dosage , Methylene Chloride/administration & dosage , Blood Glucose/drug effects , Plant Extracts/chemistry , Chromatography, High Pressure Liquid , Rats, Wistar , Streptozocin , Oxidative Stress/drug effects , Spectrometry, Mass, Electrospray Ionization , Phenolic Compounds/analysis , Hypolipidemic Agents/administration & dosage , Antioxidants/administration & dosage
12.
Braz. arch. biol. technol ; 64(spe): e21200772, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278459

ABSTRACT

Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger apparatus and the compounds were identified by GC/MS. The antioxidant activity was evaluated by DPPH, ABTS, and reducing phosphomolybdenum complex. Broth microdilution was used to determine antimicrobial activity. Cytotoxicity was verified against HeLa, HRT-18, and Calu-3 cells by MTT assay. The cytotoxic mechanism was studied by cell DNA content, cell cycle, and DNA fragmentation. The microscopic analyzes of the leaves and the stems were performed by light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The main constituent of the EO was 1,8-cineole (55.24%). The EO showed low antioxidant and antimicrobial activities. Calu-3 cells showed a significant reduction in viability with IC50 of 689.79 ± 29.34 μg/mL. EO at 1000 μg/mL decreased the DNA content in Jurkat cells. In general, EO increased cell percentage in sub-G0 and S phases with concomitant reduction of cell percentage in G0/G1 and G2/M phases and provided DNA fragmentation of 29.73%. Anatomical and micromorphological features of the leaves and stems can help in the species identification and its differentiation from other Eucalyptus species.


Subject(s)
Terpenes , Biological Phenomena , Oils, Volatile , Myrtaceae , Microscopy
13.
Braz. arch. biol. technol ; 64: e21200663, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278440

ABSTRACT

Abstract Our study aimed to evaluate the chemical composition of the essential oil from the leaves of Eugenia pyriformis Cambess., belonging to the Myrtaceae family and native to the Brazilian Atlantic forest. The volatile compounds in the essential oil were extracted by hydrodistillation and analyzed using GC-MS; 36 compounds accounted for 78.80% of the total oil content. The major compounds were β-caryophyllene, bicyclogermacrene, globulol, and (δ-cadinene. We evaluated their antimicrobial potential of the essential oil and toxicity to Artemia salina. The antimicrobial activity of the essential oil was evaluated against 12 microorganisms using the broth microdilution method. Our results showed moderate inhibition of Staphylococcus aureus and methicillin-resistant S. aureus (MIC, 250 and 125 μg.mL-1, respectively) and toxicity to A. salina (LC50, 125.64 μg.mL-1). Our results establish the biological activity of the essential oil obtained from E. pyriformis.


Subject(s)
Oils, Volatile , Myrtaceae/chemistry , Drug Resistance, Microbial , Anti-Infective Agents
14.
Braz. j. biol ; 80(4): 777-782, Oct.-Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1142524

ABSTRACT

Abstract The objective of this study was to evaluate the internal morphology of seeds of Campomanesia pubescens (Mart. ex DC.) O. Berg with varying water content through X-ray image analysis, and correlate the images with the germination of these seeds. Fruits were collected in Jataí, Goiás, Brazil, and taken to the Seed Laboratory of the Federal Institute Goiano, Rio Verde Campus, where they were processed and the seeds were extracted. To establish the predetermined water content (43%, 37%, 34%, 31%, and 28% wet basis), seeds with an initial water content of 43% were kept in an oven with forced air circulation at a temperature of 40 °C until they reached the predetermined water content levels. Next, the seeds were placed in acrylic holders with double-sided tape and transported to the Federal University of Lavras (UFLA), where they were exposed to different intensities of radiation. The X-ray test was conducted with 200 seeds per treatment, and images of the internal structures of the seeds were evaluated. The seeds were returned to the Seed Laboratory of the Federal Institute Goiano, Rio Verde Campus, where the germination test was initiated. The experimental design was completely randomized, in a factorial design (5 × 4), in which there were five water content levels and four seed classes, with four replications. The X-ray test was efficient in the identification of filled, malformed, damaged and empty seeds, which germination rate above 50% in the filled seeds. This visualization of the internal morphology of the seed can be useful to select seeds of better quality, improving the percentage of germination of C. pubescens seeds.


Resumo O objetivo deste trabalho foi avaliar a morfologia interna de sementes de Campomanesia pubescens (Mart. ex DC.) O.Berg com diferentes teores de água por meio de análise de imagens de raios-X, e correlacionar as imagens com a germinação dessas sementes. Os frutos foram coletados no município de Jataí, Goiás, Brasil, e levados ao Laboratório de Sementes do Instituto Federal Goiano, Campus Rio Verde, onde foram processados ​​e as sementes foram extraídas. Para estabelecer o teor de água predeterminado (43%, 37%, 34%, 31% e 28% base úmida), as sementes com um teor inicial de água de 43% foram mantidas em estufa com circulação de ar induzida, a uma temperatura de 40 °C até atingirem os níveis de conteúdo de água predeterminados. Em seguida, as sementes foram colocadas em suportes acrílicos com fita dupla face e transportadas para a Universidade Federal de Lavras (UFLA), onde foram expostas a diferentes intensidades de radiação. O teste de raios-X foi realizado com 200 sementes por tratamento, e imagens das estruturas internas das sementes foram avaliadas. As sementes foram devolvidas ao Laboratório de Sementes do Instituto Federal Goiano, Campus Rio Verde, onde foi iniciado o teste de germinação. O delineamento experimental foi inteiramente casualizado, em esquema fatorial (5 × 4), no qual foram encontrados cinco níveis de umidade e quatro classes de sementes, com quatro repetições. O teste de raios-X foi eficiente na identificação de sementes preenchidas, malformadas, danificadas e vazias, com taxas de germinação acima dos 50% nas sementes cheias. Essa visualização das morfologia interna da semente pode ser útil para selecionar sementes de melhor qualidade, melhorando a porcentagem de germinação das sementes de C. pubescens.


Subject(s)
Germination , Myrtaceae , Seeds , Temperature , Brazil , Desiccation
15.
Braz. j. biol ; 80(3): 601-606, July-Sept. 2020. graf
Article in English | LILACS | ID: biblio-1132409

ABSTRACT

Abstract Campomanesia phaea (Myrtaceae), popularly known as cambuci, is one of several species of plants producing comestible fruits, largely used in human nutrition. Despite its consumption and economic potential, limited scientific research is available on the Campomanesia, especially those related to its therapeutic benefits. It is reported by traditional medicine the use of the plant in the treatment of different disorders, such as cardiovascular and nervous system disturbances. So, the aim of this study was to carry out the pharmacological evaluation of the hydro-alcoholic extract (HAE) of Campomanesia fruits in rats by screening consisting of tests: a) neuropharmacological observation, b) test on the cardiovascular system. The HAE, prepared from the extraction of fruits with water/ethanol, was concentrated and freeze-dried. Behavioral responses in rats were investigated in open field test and the cardiovascular actions were investigated by a register of indirect blood pressure and the register of spontaneous beating rate right atrium. The results revealed that HAE induced grooming, hypotension and bradycardia. So, this study identified an action on the central nervous system, represented by grooming, and a cardiovascular activity of Campomanesia. The hypotension, attributed in part to bradycardia, was not related to a cholinergic effect, discarding a possible cholinomimetic action of the plant that could justify both cardiovascular and central actions.


Resumo Campomanesia phaea (Myrtaceae), popularmente conhecida como cambuci, é uma das várias espécies de plantas que produzem frutos comestíveis amplamente utilizados na nutrição humana. Apesar de seu consumo e potencial econômico, há poucas pesquisas científicas sobre a Campomanesia, especialmente aquelas relacionadas aos seus benefícios terapêuticos. É relatado pela medicina tradicional o uso da planta no tratamento de diferentes distúrbios, tais como distúrbios do sistema nervoso e cardiovascular. Assim, o objetivo deste estudo foi realizar a avaliação farmacológica do extrato hidroalcoólico (EHA) dos frutos de Campomanesia phaea em ratos através de triagem composta pelos testes: a) observação neurofarmacológica, b) testes no sistema cardiovascular. O EHA, preparado a partir da extração de frutos com água/etanol, foi concentrado e liofilizado. As respostas comportamentais em ratos foram investigadas em teste de campo aberto e as ações cardiovasculares foram investigadas pelo registro da pressão arterial indireta e o registro da taxa de batimentos cardíacos espontâneos em preparações isoladas de átrio direito. Os resultados revelaram que EHA induziu grooming, hipotensão e bradicardia. Assim, este estudo identificou uma ação da Campomanesia sobre o sistema nervoso central, representada por grooming, e uma atividade cardiovascular. A hipotensão, atribuída em parte à bradicardia, não está relacionada a um efeito colinérgico, descartando uma possível ação colinomimética da planta que pudesse justificar tanto as ações cardiovasculares quanto as centrais.


Subject(s)
Humans , Animals , Rats , Myrtaceae , Fruit , Plant Extracts , Rats, Wistar , Ethanol
16.
Braz. j. biol ; 80(2): 290-294, Apr.-June 2020. tab
Article in English | LILACS | ID: biblio-1132385

ABSTRACT

Abstract Essential oils, which may be extracted from several parts of plants, have different biological activities. The Brazilian Cerrado has a large variety of plants that yield essential oils, even though many have not been studied yet. Taking into account the biodiversity of this biome, this study aimed at evaluating the antiproliferative activity of essential oils extracted from three species of plants of the Cerrado in Goiás state: Campomanesia adamantium (Cambess.) O. Berg, Protium ovatum (Engl. in Mart.) and Cardiopetalum calophyllum (Schltdl.). Essential oils were extracted from both C. adamantium and C. calophyllum leaves and from P. ovatum leaves and green fruits by hydrodistillation carried out by a Clevenger-type apparatus. The chemical composition of the essential oils was determined by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The following major chemical constituents were identified in the essential oils under investigation: β-myrcene (62.00%), spathulenol (28.78%), germacrene-B (18.27%), β-caryophyllene oxide (16.40%), β-caryophyllene (14.00%), α-pinene (11.30%), viridiflorol (9.99%), limonene (7.30%) and (Z,E)-pharnesol (6.51%). The antiproliferative activity was evaluated in different human tumor cell lines: breast adenocarcinoma (MCF-7), cervical adenocarcinoma (HeLa) and glioblastoma (M059J). A normal human cell line was included (GM07492A, lung fibroblasts). Results showed that essential oils from C. adamantium leaves got the lowest values of IC50 in all strains of tumor cells under evaluation. They were significantly lower than the ones of the normal cell line, an evidence of selectivity. It is worth mentioning that this is the first report of the antiproliferative activity of essential oils from C. adamantium , P. ovatum and C. calophyllum against human tumor cells.


Resumo Os óleos essenciais podem ser extraídos de várias partes das plantas e apresentam diversas atividades biológicas. O Cerrado brasileiro possui uma grande variedade de plantas produtoras de óleos essenciais muitas delas ainda não estudadas. Levando-se em consideração a biodiversidade desse bioma, o objetivo deste trabalho foi avaliar a atividade antiproliferativa dos óleos essenciais extraídos de três espécies de plantas ocorrentes no Cerrado do estado de Goiás: Campomanesia adamantium (Cambess.) O. Berg, Protium ovatum (Engl. in Mart.) e Cardiopetalum calophyllum (Schltdl.). Os óleos essenciais foram obtidos das folhas de C. adamantium e C. calophyllum e das folhas e frutos verdes de P. ovatum por hidrodestilação, usando o aparelho do tipo Clevenger. A composição química dos óleos essenciais foi determinada pelo método de Cromatografia Gasosa acoplada à Espectrometria de Massas (CG-EM). Os constituintes químicos majoritários identificados nos óleos essenciais estudados foram: β-mirceno (62,00%), espatulenol (28,78%), germacreno-B (18,27%), óxido de β-cariofileno (16,40%), β-cariofileno (14,00%), α-pineno (11,30%), viridiflorol (9,99%), limoneno (7,30%) e (Z,E)-farnesol (6,51%). A atividade antiproliferativa foi avaliada em diferentes linhagens de células tumorais humanas: adenocarcinoma de mama (MCF-7), adenocarcinoma cervical (HeLa) e gliobastoma (M059J), além de, uma linhagem celular humana normal (GM07492A, fibroblastos pulmonares). O óleo essencial das folhas de C. adamantium exibiu menores valores de CI50 em todas as linhagens celulares tumorais avaliadas, sendo menores que aquele obtido na linhagem celular normal, indicando seletividade. Este é o primeiro relato da atividade antiproliferativa dos óleos essenciais de C. adamantium , P. ovatum e C. calophyllum contra células tumorais humanas.


Subject(s)
Humans , Oils, Volatile , Annonaceae , Burseraceae , Myrtaceae , Calophyllum , Brazil , Plant Leaves , Hydrogen
17.
Biosci. j. (Online) ; 36(2): 568-577, 01-03-2020. tab
Article in English | LILACS | ID: biblio-1146421

ABSTRACT

In the Myrtaceae family, the species Eugenia involucrata DC., popularly known as "cerejeira-do-mato", is traditionally used for the antidiarrheal and digestive action of its leaves. However, no studies were found in the literature regarding its antimicrobial and antioxidant potential. In this context, the objective of the present study was to determine the chemical composition by gas chromatography coupled to mass spectrometry (GC-MS) to evaluate the antimicrobial activity by the broth microdilution technique and the antioxidant activity by the 2,2-diphenyl-1-picryl-hydrazila (DPPH) method of the essential oil of E. involucrataleaves. GC-MS identified 28 compounds, all sesquiterpenes, corresponding to 89.41% of the essential oil. The antimicrobial activity of the essential oil was observed for all Gram-positive bacteria tested (Staplylococcus epidermidis, Enterococcus faecalis,Bacillus subtilis and Staplylococcus aureus) and for yeast Candida albicans. The essential oil presented a reduction capacity of DPPH up to 66.81%, evidencing its antioxidant potential. It is suggested that the antimicrobial and antioxidant action of E. involucrata essential oil is related to the presence of the major compounds, elixene (26.53%), ß-caryophyllene (13.16%), α-copaene (8.41%) and germacrene D (7.17%).


Na família Myrtaceae, a espécieEugenia involucrata DC. popularmente denominada "cerejeira-do-mato" é conhecida tradicionalmente pela ação antidiarreica e digestiva de suas folhas. Contudo, na literatura não foram encontrados trabalhos referentes ao seu potencial antimicrobiano e antioxidante. Neste contexto, o objetivo do presente estudo foi determinar a composição química por cromatografia gasosa acoplada a espectrometria de massas (CG-EM) e avaliar a atividade antimicrobiana pela técnica de microdiluição em caldo e a atividade antioxidante pelo método do 2,2-difenil-1-picril-hidrazila (DPPH) do óleo essencial das folhas de E. involucrata. A CG-EM identificou 28 compostos, todos sesquiterpenos, correspondendo a 89,41% do óleo essencial. A atividade antimicrobiana do óleo essencial foi observada para todas as bactérias Gram-positivas testadas (Staplylococcus epidermidis, Enterococcus faecalis,Bacillus subtilise Staplylococcus aureus) e para a levedura Candida albicans. O óleo essencial apresentou capacidade redutora de radicais DPPH de até 66,81%, evidenciando sua potencialidade antioxidante. Sugere-se que a ação antimicrobiana e antioxidante do óleo essencial de E. involucrata esteja relacionada à presença dos compostos majoritários, elixeno (26,53%), ß-cariofileno (13,16%), -copaeno (8,41%) e germacreno D (7,17%).


Subject(s)
Myrtaceae , Eugenia , Anti-Infective Agents , Antioxidants , Mass Spectrometry , Bacillus , Bacteria , Biological Products , Candida albicans , Oils , Distillation , Chromatography, Gas , Enterococcus faecalis , Volatile Organic Compounds
18.
Article in English | MTYCI, LILACS, MTYCI | ID: biblio-1145994

ABSTRACT

El presente estudio tiene como objetivo explorar las posibles aplicaciones de los extractos de corteza y hoja de Blepharocalyx cruckshanksii como agente citotóxico contra líneas celulares de cáncer in vitro ((MCF-7, PC-3 y HT-29) mediante el uso de ensayo de sulforhodamine B (SRB). El ensayo de citotoxicidad reveló que el extracto de acetato de etilo de la corteza exhibía una actividad anticancerígena marcada. El extracto activo se sometió a un reparto líquido-líquido usando hexano y acetato de etilo para obtener fracciones basadas en su polaridad. Sin embargo, la Fracción 4 (F4) fue identificado como el más efectivo de la serie al mostrar contra todas las líneas celulares de cáncer una citotoxicidad cercana a los agentes antineoplásicos ensayados. Luego, F4 se analizó por cromatografía de gasesespectrometría de masas (GC-MS) para identificar sus componentes principales y relacionar estos componentes con el efecto citotóxico. Los resultados obtenidos indicaron que la corteza de B. cruckshanksii tiene una excelente actividad citotóxica y amerita estudios adicionales para aislar nuevos compuestos para quimioterapia.


The present study aims to explore the potential applications of Blepharocalyx cruckshanksii bark and leaf extracts as a cytotoxic agent against in vitro cancer cell lines (MCF-7, PC-3 and HT-29) by using sulforhodamine B (SRB) assay. The cytotoxicity assay revealed that the ethyl acetate extract from the bark exhibited marked anticancer activity. The active extract was subjected to liquid-liquid partitioning by using hexane and ethyl acetate to obtain fractions based on their polarity. However, Fraction 4 (F4) was identified as the most effective of the series by displaying against all cancer cell lines a cytotoxicity close to antineoplastic agents assayed. Then, F4 was analyzed by gas chromatography­mass spectrometry (GC-MS) to identify their major components and to relate these components to the cytotoxic effect. The results obtained indicated that B. cruckshanksii bark have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.


Subject(s)
Humans , Myrtaceae/chemistry , Cytotoxins , Antineoplastic Agents , In Vitro Techniques , Plant Extracts , Chile , Chromatography, Gas
19.
Rev. peru. med. integr ; 5(2): 49-54, 2020. tab
Article in Spanish | MTYCI, LILACS, MTYCI | ID: biblio-1146105

ABSTRACT

Objetivos. Determinar la estabilidad de cremas fotoprotectoras a base de Myrcianthes rhopaloides «lanche colorado¼ de los páramos de Piura. Materiales y métodos. Se evaluaron tres extractos hidroetanólicos y dos acuosos que fueron purificados con Amberlite® XAD7HP. Se formularon cremas fotoprotectoras con los purificados para la realización de la prueba de shock térmico, la evaluación de los parámetros organolépticos y fisicoquímicos, antes y después del almacenamiento. Se escogieron las mejores formulaciones para la realización de la prueba de estabilidad acelerada. Resultados. No se evidenciaron cambios organolépticos y fisicoquímicos entre las formulaciones. Se optó por escoger la crema a base de purificado de 45%, a la cual se realizó la prueba de estabilidad acelerada; mostrando cambios organolépticos a los siete días, y cambios fisicoquímicos durante todo el tiempo de almacenamiento. Conclusiones. Se determinó que los parámetros físicos y químicos de la crema fotoprotectora a base de extracto purificado de 45% presenta moderada estabilidad.


Objectives. To determine the stability of photoprotective creams based on Myrcianthes rhopaloides "lanche colorado" from the paramos of Piura. Materials and methods. Three hydroethanolic and two aqueous extracts were evaluated that were purified with Amberlite® XAD7HP. Photoprotective creams were formulated with the purified ones for the thermal shock test, the evaluation of the organoleptic and physicochemical parameters, before and after storage. The best formulations were chosen to perform the accelerated stability test. Results. There were no organoleptic and physicochemical changes between the formulations. It was decided to choose the cream based on purification of 45%, to which the accelerated stability test was carried out; showing organoleptic changes at 7 days and physicochemical changes throughout the storage time. Conclusions. It was determined that the physical and chemical parameters of the photoprotective cream based on 45% purified extract presents moderate stability.


Subject(s)
Humans , Sunscreening Agents , Myrtaceae/chemistry , Peru , Plant Extracts , Freeze Drying
20.
Rev. cuba. estomatol ; 56(4): e1779, oct.-dez. 2019.
Article in Spanish | LILACS | ID: biblio-1093254

ABSTRACT

RESUMEN Introducción: El tratamiento de la enfermedad periodontal incluye la terapia mecánica, el cual se complementa con el uso de antibióticos/antisépticos, lo que podría plantear efectos adversos a largo plazo. Objetivo: Describir el potencial farmacológico del fruto de la M. dubia, y su aplicación como complemento en la terapia periodontal. Métodos: Se revisaron revistas internacionales de impacto de la Web of Science relacionadas con el tema (58 revistas). Se consultaron las bases de datos Google Académico, SciELO, PubMed y EBSCO, utilizando los descriptores: "review"; "phytotherapy"; "myrtaceae"; "gingivitis"; "periodontitis"; "periodontal diseases"; "anti-bacterial agents"; "anti-inflammatory agents"; "dental plaque"; "antioxidants"; "plants, toxic"; "adverse effects". Se obtuvo 517 artículos de los cuales 60 fueron incluidos en el estudio. El 91,7 por ciento de los artículos fueron de los últimos tres años. Integración de la información: Se expuso las propiedades y seguridad en humanos del uso de la M. dubia. Conclusiones: La M. dubia tiene actividad antimicrobiana in vitro frente a microorganismos de la biopelícula dental, siendo más sensibles el S. mutans, S. mitis y P. gingivalis al extracto hidroalcohólico de la semilla, y el S. aureus al extracto hidroalcohólico de las hojas y corteza. También se evidencia su actividad antiinflamatoria. Los hallazgos sugieren que el extracto etanólico de la M. dubia podría incorporarse en antisépticos de uso bucal, dado su potencial antibiopelícula y antiinflamatoria(AU)


ABSTRACT Introduction: Treatment of periodontal disease includes mechanical therapy complemented with antibiotics / antiseptics, which could lead to the occurrence of long-term adverse effects. Objective: Describe the pharmacological potential of the fruit of M. dubia and its use as adjunct in periodontal therapy. Methods: A review was conducted of international high impact journals from the Web of Sciences which dealt with the topic (58 journals). The databases Google Scholar, SciELO, PubMed and EBSCO were consulted, using the descriptors "review", "phytotherapy", "Myrtaceae", "gingivitis", "periodontitis", "periodontal diseases", anti-bacterial agents", "anti-inflammatory agents", "dental plaque", "antioxidants", "plants, toxic", "adverse effects". A total 517 papers were obtained, of which 60 were included in the study. 91.7 percent of the papers had been published in the last three years. Data integration: A description was provided of the properties and safety of the use of M. dubia in humans. Conclusions: M. dubia has in vitro antimicrobial activity against dental biofilm microorganisms, particularly S. mutans, S. mitis and P. gingivalis to the seed hydroalcoholic extract, and S. aureus to the leaf and peel hydroalcoholic extract. Anti-inflammatory activity was also observed. Results suggest that M. dubia ethanolic extract could be incorporated into oral antiseptics, given its antibiofilm and anti-inflammatory potential(AU)


Subject(s)
Humans , Periodontal Diseases/drug therapy , Review Literature as Topic , Myrtaceae/adverse effects , Phytotherapy/methods , Databases, Bibliographic
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