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1.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 226-243, may. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1342815

ABSTRACT

Several species of the Myrcia genus have been used in folk medicine to treat diabetes. Therefore, the aim of this work was to investigate the inhibitory activity of α-glucosidase and pancreatic lipase in the crude extract (EBF) and in the ethyl acetate fraction (FFA) of Myrcia hatschbachii, as well as to identify isolated phenolic compounds and to evaluate the antioxidant property and preliminary in vitro toxicity against Artemia salina. EBF (IC50: 3.21 µg/mL) and FFA (IC50: 1.14 µg/mL) showed inhibitory activity superior to acarbose (IC50: 193.65 µg/mL). In addition, they showed inhibitory effects of pancreatic lipase (IC50: 556.58 µg/mL for EBF and 532.68 µg/mL for FFA), antioxidant potential, absence of preliminary toxicity and presence of gallic andellagic acids in FFA. The relevant results in the inhibition of α-glucosidase and pancreatic lipase motivate new studies for the development of herbal medicines that assist in the treatment of diabetic patients.


Varias especies del género Myrcia se han utilizado en la medicina popular para tratar la diabetes. Por lo tanto, el objetivo de este trabajo fue investigar la actividad inhibitoria de la α-glucosidasa y la lipasa pancreática en el extracto crudo (EBF) y en la fracción de acetato de etilo (FFA) de Myrcia hatschbachii, así como identificar compuestos fenólicos aislados y evaluar la propiedad antioxidante y toxicidad in vitro preliminar contra Artemia salina. EBF (IC50: 3.21 µg/mL) y FFA (IC50: 1.14 µg/mL) mostraron una actividad inhibitoria superior a la acarbosa (IC50: 193.65 µg/mL). Además, mostraron efectos inhibitorios de la lipasa pancreática (IC50: 556.58 µg/mL para EBF y 532.68 µg/mL para FFA), potencial antioxidante, ausencia de toxicidad preliminar y presencia de ácidos gálico y elágico en FFA. Los resultados relevantes en la inhibición de la α-glucosidasa y la lipasa pancreática motivan nuevos estudios para el desarrollo de medicamentos a base de hierbas que ayudan en el tratamiento de pacientes diabéticos.


Subject(s)
Plant Extracts/pharmacology , Myrtaceae/chemistry , Glycoside Hydrolase Inhibitors/pharmacology , Lipase/drug effects , Antioxidants/pharmacology , Pancreas/enzymology , Phenols/analysis , X-Ray Diffraction , In Vitro Techniques , Plant Extracts/toxicity , Plant Extracts/chemistry , Free Radical Scavengers , Complex Mixtures , Ellagic Acid , Gallic Acid , Antioxidants/chemistry
2.
Bol. latinoam. Caribe plantas med. aromát ; 20(3): 260-269, may. 2021. tab
Article in English | LILACS | ID: biblio-1342820

ABSTRACT

The aim of this study was to undertake a screening experiment on essential oils (EO) of Myrcia lundiana, Ocimum basilicum and Lippia alba against six food-spoiling pathogenic bacteria. Seventy-two (72) samples were initially analyzed fo antimicrobial activity based on the agar diffusion test. The minimum inhibitory (MIC) and bactericidal (MBC) concentrations were determined for the 12 samples which showed greatest antimicrobial potential in this stage. Two samples of L. alba, three samples of M. lundiana and seven samples of O. basilicum showed a MIC of 0.12-125 µL/mL for the six tested bacteria. Of these, the EO of O. basilicum cultivar Maria Bonita stood out with the lowest MIC and MBC. Thus, a mixture simulating this essential oil was prepared from commercial standards of the compounds (±)-linalool, geraniol and 1,8-cineole. Significantly higher MIC and MBC were detected in the simulation compared to the respective EO, suggesting a synergistic effect between compounds.


El objetivo de este estudio fue realizar un experimento de detección en aceites esenciales (AE) de Myrcia lundiana, Ocimum basilicum y Lippia alba contra seis bacterias patógenas que estropean los alimentos. Setenta y dos (72) muestras fueron analizadas inicialmente para la actividad antimicrobiana basada en la prueba de difusión en agar. Se determinaron las concentraciones mínimas inhibitoria (CMI) y bactericida (CMB) para las 12 muestras que mostraron el mayor potencial antimicrobiano en esta etapa. Dos muestras de L. alba, tres muestras de M. lundiana y siete muestras de O. basilicum mostraron un CMI de 0.12-125 µL/mL para las seis bacterias analizadas. De estos, el AE de O. basilicum cultivar Maria Bonita se destacó con el CMI y CMB más bajos. Por lo tanto, se preparó una mezcla que simula este aceite esencial a partir de los estándares comerciales de los compuestos de (±)-linalol, geraniol y 1,8-cineol. Se detectaron CMI y CMB significativamente más altos en la simulación en comparación con el AE respectivo, lo que sugiere un efecto sinérgico entre los compuestos.


Subject(s)
Bacteria/drug effects , Oils, Volatile/pharmacology , Ocimum basilicum/chemistry , Myrtaceae/chemistry , Lippia/chemistry , Anti-Bacterial Agents/pharmacology , Sesquiterpenes/analysis , Oils, Volatile/chemistry , Microbial Sensitivity Tests , Food , Anti-Bacterial Agents/chemistry
3.
Bol. latinoam. Caribe plantas med. aromát ; 20(2): 132-146, 2021. ilus, tab
Article in English | LILACS | ID: biblio-1342208

ABSTRACT

We investigated the effects of dichloromethane extract (DME) from Myrcia splendenson alterations caused by type 2 diabetes in the blood and kidney of rats, in order to reduce side effects caused by synthetic drugs. Rats received streptozotocin (60 mg/kg),15 minutes after nicotinamide (120 mg/kg) or water. After 72 hours, the glycemic levels were evaluated to confirm diabetes and the animals received (15 days) DME (25, 50, 100 or 150 mg/Kg) or water. DME partially reversed hyperglycemia and (100 and 150 mg/kg) reversed hypertriglyceridemia. Histopathological findings elucidated that DME reduced damage to pancreatic islets. DME 150 mg/kgreversed the increases in TBA-RS, the reduction in the sulfhydryl content, 100 and 150 mg/kg increased CAT, reversed the decrease in GSH-Px and increased it activity in the blood. DME 150 mg/kg reversed CAT and GSH-Px reductions in the kidney. We believe that DME effects might be dependent on the presence of phenolic compounds.


Investigamos los efectos del extracto de diclorometano (DME)de Myrcia splendens sobre las alteraciones causadas por la diabetes tipo 2 en la sangre y los riñones de las ratas, para reducir los efectos secundarios causados por las drogas sintéticas. Las ratas recibieron estreptozotocina (60 mg/kg), 15 minutos después de la nicotinamida (120 mg/kg) o agua. Después de 72 horas, se confirmo la diabetes y los animales recibieron (15 días) DME (25, 50, 100 o 150 mg/Kg) o agua. DME revierte parcialmente la hiperglucemia y revierte la hipertrigliceridemia. DME redujo el daño a los islotes pancreáticos. DME revirtió los aumentos en TBA-RS, la reducción en el contenido de sulfhidrilo, aumentó la CAT, revirtió la disminución en GSH-Px y aumentó su actividad en la sangre. Además, DME revirtió las reducciones de CAT y GSH-Px en el riñón. Creemos que los efectos provocados por DME pueden depender de la presencia de compuestos fenólicos.


Subject(s)
Animals , Male , Rats , Plant Extracts/administration & dosage , Myrtaceae/chemistry , Diabetes Mellitus, Experimental/drug therapy , Hypoglycemic Agents/administration & dosage , Methylene Chloride/administration & dosage , Blood Glucose/drug effects , Plant Extracts/chemistry , Chromatography, High Pressure Liquid , Rats, Wistar , Streptozocin , Oxidative Stress/drug effects , Spectrometry, Mass, Electrospray Ionization , Phenolic Compounds/analysis , Hypolipidemic Agents/administration & dosage , Antioxidants/administration & dosage
4.
Braz. arch. biol. technol ; 64: e21200663, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278440

ABSTRACT

Abstract Our study aimed to evaluate the chemical composition of the essential oil from the leaves of Eugenia pyriformis Cambess., belonging to the Myrtaceae family and native to the Brazilian Atlantic forest. The volatile compounds in the essential oil were extracted by hydrodistillation and analyzed using GC-MS; 36 compounds accounted for 78.80% of the total oil content. The major compounds were β-caryophyllene, bicyclogermacrene, globulol, and (δ-cadinene. We evaluated their antimicrobial potential of the essential oil and toxicity to Artemia salina. The antimicrobial activity of the essential oil was evaluated against 12 microorganisms using the broth microdilution method. Our results showed moderate inhibition of Staphylococcus aureus and methicillin-resistant S. aureus (MIC, 250 and 125 μg.mL-1, respectively) and toxicity to A. salina (LC50, 125.64 μg.mL-1). Our results establish the biological activity of the essential oil obtained from E. pyriformis.


Subject(s)
Oils, Volatile , Myrtaceae/chemistry , Drug Resistance, Microbial , Anti-Infective Agents
5.
Article in English | MTYCI, LILACS, MTYCI | ID: biblio-1145994

ABSTRACT

El presente estudio tiene como objetivo explorar las posibles aplicaciones de los extractos de corteza y hoja de Blepharocalyx cruckshanksii como agente citotóxico contra líneas celulares de cáncer in vitro ((MCF-7, PC-3 y HT-29) mediante el uso de ensayo de sulforhodamine B (SRB). El ensayo de citotoxicidad reveló que el extracto de acetato de etilo de la corteza exhibía una actividad anticancerígena marcada. El extracto activo se sometió a un reparto líquido-líquido usando hexano y acetato de etilo para obtener fracciones basadas en su polaridad. Sin embargo, la Fracción 4 (F4) fue identificado como el más efectivo de la serie al mostrar contra todas las líneas celulares de cáncer una citotoxicidad cercana a los agentes antineoplásicos ensayados. Luego, F4 se analizó por cromatografía de gasesespectrometría de masas (GC-MS) para identificar sus componentes principales y relacionar estos componentes con el efecto citotóxico. Los resultados obtenidos indicaron que la corteza de B. cruckshanksii tiene una excelente actividad citotóxica y amerita estudios adicionales para aislar nuevos compuestos para quimioterapia.


The present study aims to explore the potential applications of Blepharocalyx cruckshanksii bark and leaf extracts as a cytotoxic agent against in vitro cancer cell lines (MCF-7, PC-3 and HT-29) by using sulforhodamine B (SRB) assay. The cytotoxicity assay revealed that the ethyl acetate extract from the bark exhibited marked anticancer activity. The active extract was subjected to liquid-liquid partitioning by using hexane and ethyl acetate to obtain fractions based on their polarity. However, Fraction 4 (F4) was identified as the most effective of the series by displaying against all cancer cell lines a cytotoxicity close to antineoplastic agents assayed. Then, F4 was analyzed by gas chromatography­mass spectrometry (GC-MS) to identify their major components and to relate these components to the cytotoxic effect. The results obtained indicated that B. cruckshanksii bark have excellent cytotoxic activity and warrant further studies to isolate novel compounds for chemotherapeutic use.


Subject(s)
Humans , Myrtaceae/chemistry , Cytotoxins , Antineoplastic Agents , In Vitro Techniques , Plant Extracts , Chile , Chromatography, Gas
6.
Rev. peru. med. integr ; 5(2): 49-54, 2020. tab
Article in Spanish | MTYCI, LILACS, MTYCI | ID: biblio-1146105

ABSTRACT

Objetivos. Determinar la estabilidad de cremas fotoprotectoras a base de Myrcianthes rhopaloides «lanche colorado¼ de los páramos de Piura. Materiales y métodos. Se evaluaron tres extractos hidroetanólicos y dos acuosos que fueron purificados con Amberlite® XAD7HP. Se formularon cremas fotoprotectoras con los purificados para la realización de la prueba de shock térmico, la evaluación de los parámetros organolépticos y fisicoquímicos, antes y después del almacenamiento. Se escogieron las mejores formulaciones para la realización de la prueba de estabilidad acelerada. Resultados. No se evidenciaron cambios organolépticos y fisicoquímicos entre las formulaciones. Se optó por escoger la crema a base de purificado de 45%, a la cual se realizó la prueba de estabilidad acelerada; mostrando cambios organolépticos a los siete días, y cambios fisicoquímicos durante todo el tiempo de almacenamiento. Conclusiones. Se determinó que los parámetros físicos y químicos de la crema fotoprotectora a base de extracto purificado de 45% presenta moderada estabilidad.


Objectives. To determine the stability of photoprotective creams based on Myrcianthes rhopaloides "lanche colorado" from the paramos of Piura. Materials and methods. Three hydroethanolic and two aqueous extracts were evaluated that were purified with Amberlite® XAD7HP. Photoprotective creams were formulated with the purified ones for the thermal shock test, the evaluation of the organoleptic and physicochemical parameters, before and after storage. The best formulations were chosen to perform the accelerated stability test. Results. There were no organoleptic and physicochemical changes between the formulations. It was decided to choose the cream based on purification of 45%, to which the accelerated stability test was carried out; showing organoleptic changes at 7 days and physicochemical changes throughout the storage time. Conclusions. It was determined that the physical and chemical parameters of the photoprotective cream based on 45% purified extract presents moderate stability.


Subject(s)
Humans , Sunscreening Agents , Myrtaceae/chemistry , Peru , Plant Extracts , Freeze Drying
7.
Bol. latinoam. Caribe plantas med. aromát ; 18(4): 435-443, jul. 2019. ilus, tab
Article in English | LILACS | ID: biblio-1008258

ABSTRACT

Volatiles compounds are involved in defensive induction against insects, playing an important role in insect-plant interaction being induced by response to mechanical damage. However, they could decrease according to the domestication degree in cultivated plants. Currently, it has been established that secondary metabolites are reduced due to the domestication process in murtilla. Hence, the follow question emerges: Are volatile organic compounds induced by mechanical damage reduced in cultivated murtilla plants in relation to wild plants? Two cultivated ecotypes and their respective wild counterparts were sampled. Volatiles compounds were obtained using Porapak-Q columns and analyzed by gas chromatography. Results showed that compounds as 2-hexanone, α-pinene, 2-thujene, 3-thujene and 1,8- cineole were more abundant in wild plants exposed to a mechanical damage than cultivated plants. Hence, these compounds have been associated to induced defense, these results suggest that domestication reduced the induction of defensive volatiles in cultivated murtilla in response to mechanical damage.


Los compuestos volátiles están implicados en la defensa inducida contra insectos, desempeñando un papel importante en esta interacción. Sin embargo, estos compuestos podrían disminuir según el grado de domesticación. Actualmente, se ha reportado que algunos metabolitos secundarios son reducidos en plantas de murtilla domesticadas. Por lo tanto, surge la siguiente pregunta de investigación: ¿Los compuestos orgánicos volátiles inducidos por el daño mecánico son reducidos en plantas cultivadas de murtilla en comparación con plantas silvestres? Para dos ecotipos cultivados y sus respectivas contrapartes silvestres, los compuestos volátiles fueron capturados usando columnas de Porapak-Q y las muestras analizadas por cromatografía gaseosa. Los resultados mostraron que compuestos tales como 2- hexanona, α-pineno, 2-tujeno, 3-tujeno y 1,8-cineol fueron más abundantes en plantas silvestres expuestas a daño mecánico que en cultivadas. Debido a que estos compuestos se han asociado a defensa inducida, estos resultados sugieren que la domesticación reduce la inducción de volátiles en plantas cultivadas sometidas a daño mecánico.


Subject(s)
Animals , Oils, Volatile/metabolism , Myrtaceae/metabolism , Myrtaceae/microbiology , Domestication , Insecta/physiology , Sesquiterpenes/analysis , Sesquiterpenes/metabolism , Oils, Volatile/chemistry , Myrtaceae/chemistry , Monoterpenes/analysis , Monoterpenes/metabolism , Wilderness , Larva/physiology , Gas Chromatography-Mass Spectrometry
8.
Braz. j. biol ; 79(1): 127-132, Jan.-Mar 2019. tab
Article in English | LILACS | ID: biblio-983990

ABSTRACT

Abstract In recent years, natural products with antifungal and antioxidant activities are being increasingly researched for a more sustainable alternative to the chemicals currently used for the same purpose. The plant pathogenic fungus Alternaria alternata is a causative agent of diseases in citrus, leading to huge economic losses. Antioxidants are important for the production of medicines for various diseases that may be related to the presence of free radicals, such as cancer, and in the cosmetic industry as an anti-aging agent and the food industry as preservatives. This study evaluated the antifungal and antioxidant potential of extracts of mature leaves of Myrcia splendens, a tree species that occurs in the Brazilian Cerrado. The antioxidant potential was analyzed by an assay of 1,1-diphenyl-2-picrylhydrazyl radical-scavenging method, and the antifungal activity was assessed through the evaluation of mycelial growth. Majority of the extracts exhibited a strong antioxidant activity, especially the acetonic extract (4A). The antioxidant activity may be related to the presence of phenolic compounds. However, the extracts showed no inhibitory activity of mycelial growth of the fungus tested, with the exception of dichloromethanic extract (2B), which had an inhibitory effect (10.2%) at the end of testing.


Resumo A busca de produtos naturais com atividades antifúngica e antioxidante tem crescido nos últimos anos como alternativa mais sustentável para os produtos químicos atualmente usados para estas funções. O fungo fitopatogênico Alternaria alternata é agente causador de doenças nos citros, levando a grandes perdas econômicas. Substâncias antioxidantes são importantes tanto para a produção de medicamentos para diversas doenças que podem estar relacionadas à presença de radicais livres, como o câncer, bem como para a indústria cosmética, como agentes anti-envelhecimento e para a indústria alimentícia, como conservantes. Este trabalho avaliou o potencial antifúngico dos extratos de folhas maduras de Myrcia splendens , uma espécie arbórea que ocorre no cerrado brasileiro. O potencial antioxidante foi analisado por meio de ensaio da capacidade sequestrante do radical 1,1-diphenyl-2-picrylhydrazyl e o antifúngico, por meio da avaliação do crescimento micelial. A maioria dos extratos apresentou atividade antioxidante muito forte, especialmente o extrato acetônico (4A). A atividade antioxidante pode ser relacionada a presença de compostos fenólicos. Por outro lado, os extratos não apresentaram atividade inibitória do crescimento micelial do fungo testado, com exceção do extrato diclorometânico (2B), que foi o único que teve efeito inibitório (10,2%) ao final do teste.


Subject(s)
Myrtaceae/chemistry , Antifungal Agents/pharmacology , Antioxidants/pharmacology , Brazil , Plant Extracts/pharmacology , Plant Extracts/chemistry , Plant Leaves/chemistry , Antifungal Agents/isolation & purification , Antioxidants/isolation & purification
9.
Rev. Col. Bras. Cir ; 46(4): e2238, 2019. graf
Article in Portuguese | LILACS | ID: biblio-1020367

ABSTRACT

RESUMO Objetivo: comparar os efeitos do extrato hidroalcoólico da casca do Camu-Camu, uma planta frutífera pertencente à família Myrtaceae amplamente distribuída na bacia amazônica, com os da gastrectomia vertical, sobre o peso e a glicemia de ratos Wistar. Métodos: vinte e quatro ratos Wistar foram submetidos à indução de obesidade através de dieta hiperlipídica por oito semanas (período de engorda), e randomizados em três grupos: Grupo Controle (GC), Grupo Camu-Camu (GCC) e Grupo Cirurgia Bariátrica (GCB). Após esse período, todos os animais retornaram a uma dieta normal e iniciou-se o período de intervenção: o GC não sofreu nenhuma intervenção além da mudança da dieta; o GCC foi submetido ao procedimento de gavagem para administração de extrato hidroalcoólico de Camu-Camu 1g/kg/dia por quatro semanas; e o GCB foi submetido ao procedimento cirúrgico de gastrectomia vertical. Todos os animais foram acompanhados por quatro semanas. Resultados: houve apenas uma perda no GCB devido à fístula gástrica. Observou-se variações significativas no peso médio dos animais: o GC evoluiu com aumento de peso mesmo após a retirada da dieta de engorda, enquanto os outros dois grupos apresentaram redução de peso. O GCB apresentou redução significativa do peso e do IMC (p<0,05); o GCC obteve redução significativa apenas do IMC (p<0,05). Não houve alterações estatisticamente significantes nos níveis glicêmicos. Conclusão: apesar de reduzir o peso, o extrato hidroalcoólico bruto da casca do Camu-Camu não foi capaz de se mostrar tão eficiente quanto a cirurgia de gastrectomia vertical no controle do peso corporal em ratos Wistar.


ABSTRACT Objective: to compare the effects of the hydroalcoholic extract from the peel of Camu-Camu, a fruit plant belonging to the Myrtaceae family, widely distributed in the Amazon basin, with those of sleeve gastrectomy, on the weight and glycemia of Wistar rats. Methods: twenty-four Wistar rats underwent obesity induction through a hyperlipid diet for eight weeks (fat period), and were randomized into three groups: Control Group (CG), Camu-Camu Group (CCG) and Bariatric Surgery Group (BSG). After this period, all animals returned to a normal diet and the intervention period began: CG did not undergo any intervention beyond diet change; CCG animals underwent gavage procedure for administration of Camu-Camu hydroalcoholic extract, 1g/kg/day, for four weeks; and the BSG was submitted to the surgical procedure of sleeve gastrectomy. We followed all animals for four weeks. Results: there was only one loss in BSG due to a gastric fistula. We observed significant variations in the animals' mean weight: the CG evolved with weight gain even after the withdraw of the hypercaloric diet, while the other two groups presented weight reduction. BSG presented a significant reduction of weight and BMI (p<0.05); CCG achieved a significant reduction only of the BMI (p<0.05). There were no statistically significant changes in the glycemic levels. Conclusion: in spite of reducing weight, the crude hydroalcoholic extract of the Camu-Camu peel was not able to be as efficient as sleeve gastrectomy in the control of body weight in Wistar rats.


Subject(s)
Animals , Male , Rats , Weight Loss , Myrtaceae/chemistry , Bariatric Surgery , Obesity/diet therapy , Rats, Wistar , Dietary Supplements , Nutrition Therapy , Disease Models, Animal , Fruit/chemistry , Obesity/surgery , Obesity/etiology
10.
Bol. latinoam. Caribe plantas med. aromát ; 16(4): 385-397, jul. 2017. ilus, tab
Article in English | LILACS | ID: biblio-882137

ABSTRACT

Myrcianthes is a Myrtaceous genus of flowering plants of about 30 to 40 species, distributed in the American continent. The aim of this work was to study the chemical composition of the foliar essential oil from M. fragrans growing wild in central Costa Rica. The essential oil was obtained through the steam distillation process in a Clevenger type apparatus. The chemical composition of the oil was performed by capillary gas chromatography with a flame detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) using the retention indices on a DB-5 type capillary column in addition to mass spectral fragmentation patterns. A total of 98 compounds were identified, accounting for 98.8% of the total amount of the oil. The major constituents in the leaf oil were (E)-methyl cinnamate (39.6%), limonene (34.6%), α-pinene (6.8%), and linalool (6.8%). This is the first report of (E)-methyl cinnamate occurring in oils of this plant genus. These findings appear to suggest a new chemotype of M. fragrans.


Myrcianthes (Myrtaceae) consta de 30 a 40 especies, distribuidas en el continente americano. El objetivo del presente trabajo consistió en identificar la composición química del aceite esencial contenido en las hojas de M. fragrans, planta que crece en forma silvestre en el Valle Central de Costa Rica. La extracción del aceite se efectuó mediante el método de hidrodestilación usando un equipo de Clevenger modificado. La composición química del aceite se analizó mediante las técnicas de cromatografía gaseoso-líquida con detector de ionización de llama (GC-FID) y de cromatografía gaseoso-líquida acoplada a un detector de masas (GC-MS). Se utilizaron índices de retención obtenidos en una columna capilar tipo DB-5 y se compararon con los patrones de iones de fragmentación de masas. Se identificaron en total 98 compuestos, correspondientes a un 98.8% de los constituyentes totales. Los componentes mayoritarios del aceite resultaron ser (E)-cinamato de metilo (39.6%), limoneno (34.6%), α-pineno (6.8%) y linalol (6.8%). Este es el primer informe de la aparición de (E)-cinamato de metilo en aceite de hojas de este género de plantas. Los datos obtenidos parecen sugerir un nuevo quimiotipo de M. fragrans.


Subject(s)
Cinnamates/analysis , Myrtaceae/chemistry , Oils, Volatile/chemistry , Plant Leaves/chemistry , Terpenes/analysis , Chromatography, Gas/methods , Costa Rica , Cyclohexanes/analysis
11.
Arq. gastroenterol ; 54(2): 135-138, Apr.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-838830

ABSTRACT

ABSTRACT BACKGROUND Given the increase of people with gastrointestinal disorders, the search for alternative treatments with fewer side effects is vital, as well as the demand for food or plants that can help protect the stomach. OBJECTIVE The aim of this study was to evaluate the gastroprotective action of the extracts of wild fruit trees of Myrcianthes pungens (guabiju); Inga vera Willd. (ingá-banana) and Marlierea tomentosa Cambess. (guarapuruna) in in vivo pharmacological models. METHODS The different parts of the fruits were separately subjected to a process of extraction by methanol. Two experimental pharmacological models were conducted in mice; the gastric ulcer model induced by non-steroidal anti-inflammatory (indomethacin), and the gastric ulcer model induced by ethanol/HCl, which allowed us to evaluate the gastroprotective activity of the extracts at a dose of 250 mg/kg. Subsequently, the total lesion area (mm2) and relative lesion area (%) were determined. RESULTS The results showed significant gastroprotective activity against the aggressive agents used - ethanol and indomethacin - for all the extracts tested. CONCLUSION It is assumed that the fruits have bioactive compounds such as antioxidant substances that act on the prostaglandin levels, protecting them from the damage caused by ethanol and indomethacin. These results prompt further studies to isolate and identify the active properties.


RESUMO CONTEXTO Devido ao aumento de pessoas com distúrbios gastrointestinais, a busca de tratamentos alternativos com menos efeitos cola­terais é fundamental, assim como a demanda por alimentos ou plantas que possam ajudar a proteger o estômago. OBJETIVO O presente estudo teve como objetivo avaliar a ação gastroprotetora dos extratos plantas frutíferas silvestres Myrcianthes pungens (guabiju); Inga vera Willd. (ingá-banana) e Marlierea tomentosa Cambess. (guarapuruna) em modelos farmacológicos in vivo. MÉTODOS As diferentes partes do fruto foram submetidas se­paradamente a um processo de maceração em solução metanólica a frio. Foram realizados dois modelos experimentais em camundongos, modelo de úlcera gástrica induzida por anti-inflamatório não-esteroidal (indometacina) e modelo de úlcera gástrica induzida por etanol/HCl, que permitiram avaliar a atividade gastroprotetora dos extratos na dose de 250 mg/kg. Posteriormente, foram determinadas a área total de lesão (mm2) e a área relativa lesada (%). RESULTADOS Os resultados apresentaram atividade gastroprotetora significativa para todos os extratos estudados frente aos agentes agressores utilizados, etanol e indometacina. CONCLUSÃO Supõe-se que os frutos apresentam compostos bioativos, como as substancias antioxidantes, que atuam sobre os níveis de prostaglandinas, protegendo dos danos causados pelo etanol e indometacina. Os resultados encorajam futuros estudos para isolamento e identificação dos princípios ativos dos frutos.


Subject(s)
Animals , Male , Mice , Stomach Ulcer/prevention & control , Plant Extracts/pharmacology , Protective Agents/pharmacology , Myrtaceae/chemistry , Fruit/chemistry , Fabaceae/chemistry , Stomach Ulcer/chemically induced , Indomethacin , Myrtaceae/classification , Disease Models, Animal , Ethanol , Fruit/classification , Gastric Mucosa/drug effects , Fabaceae/classification
12.
An. acad. bras. ciênc ; 89(2): 1111-1120, Apr.-June 2017. tab, graf
Article in English | LILACS | ID: biblio-886682

ABSTRACT

ABSTRACT Myrciaria floribunda (H. West ex Willd.) O. Berg, Myrtaceae, is a native plant species of the Atlantic Rain Forest, from north to south of Brazil. The lyophilized ethyl acetate extract from the leaves of M. floribunda was investigated for its antiproliferative activity in tumor cell lines, antioxidant capacity and its total phenolic, flavonoid and tannin contents. Antiproliferative activity was tested in vitro against seven human cancer cells and against immortalized human skin keratinocytes line (HaCat, no cancer cell). Antioxidant activity was determined using 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and oxygen radical absorbing capacity (ORAC) assays and total phenolic, flavonoid and tannin contents were determined by spectrophotometric techniques. Ethyl acetate extract of M. floribunda exhibited antiproliferative activity against cancer cell lines with total growth inhibition (TGI) between 69.70 and 172.10 µg/mL. For HaCat cell, TGI value was 213.60 µg/mL. M. floribunda showed a strong antioxidant potential: EC50 of 45.89±0.42 µg/mL and 0.55±0.05 mmol TE/g for DPPH and ORAC, respectively. Total phenolic content was 0.23±0.013g gallic acid equivalents (GAE)/g extract and exhibited 13.10±1.60% of tannins content. The content of flavonoid was 24.08±0.44% expressed as rutin equivalents. These results provide a direction for further researches about the antitumoral potential of M. floribunda.


Subject(s)
Humans , Phenols/analysis , Flavonoids/analysis , Myrtaceae/chemistry , Cell Proliferation/drug effects , Antioxidants/pharmacology , Picrates , Biphenyl Compounds , Brazil , Cell Line, Tumor , Oxygen Radical Absorbance Capacity , Indicators and Reagents , Acetates/pharmacology , Acetates/chemistry
13.
Rev. Soc. Bras. Med. Trop ; 49(5): 586-592, Sept.-Oct. 2016. tab, graf
Article in English | LILACS | ID: lil-798117

ABSTRACT

Abstract INTRODUCTION: Malaria and leishmaniasis are prevalent in tropical regions, which have environmental characteristics that are highly favorable to protozoa and vectors of these diseases; the transmission of these infections in sub-tropical regions, although recognized, represents only a small fraction of cases. Plants are constantly being used in the search for and acquisition of new drugs, and many compounds derived from them have been used to combat various diseases. In this study, we evaluated the action of the dichloromethanolic extract of Myrciaria dubia leaves against the protozoa Plasmodium falciparum, Leishmania amazonensis, Leishmania braziliensis, and Leishmania chagasi through bioassays. METHODS The extract from M. dubia was tested for its anti-P. falciparum activity in an anti-histidine-rich protein II immunosorbent assay. The antileishmanial assays were performed using the resazurin method, while cytotoxicity against human hepatoma (HepG2) strain was determined using the colorimetric MTT [3-(4, 5-dimethyl-2- thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide] method. RESULTS The M. dubia extract presented a half-maximal inhibitory concentration equal to 2.35 (1.05)μg/mL for P. falciparum, 190.73 (6.41) μg/mL for L. amazonensis, and greater than equal to 200µg/mL for L. chagasi and L. braziliensis strains. The cytotoxic concentration for 50% of the cells was above 500μg/mL for HepG2, indicating no toxicity and greater selectivity against parasites. CONCLUSIONS The results obtained indicate the presence of antiplasmodial and leishmanicidal bioactive compounds in the dichloromethanolic extracts of M. dubia leaves, and point towards future studies to elucidate the mechanism of action for each physiological effect.


Subject(s)
Humans , Plasmodium falciparum/drug effects , Plant Extracts/pharmacology , Myrtaceae/chemistry , Leishmania/drug effects , Antimalarials/pharmacology , Antiprotozoal Agents/pharmacology , Plant Extracts/toxicity , Immunoenzyme Techniques , Colorimetry , Inhibitory Concentration 50 , Parasitic Sensitivity Tests , Hep G2 Cells/drug effects , Leishmania/classification , Antimalarials/isolation & purification , Antimalarials/toxicity , Antiprotozoal Agents/isolation & purification , Antiprotozoal Agents/toxicity
14.
Arq. bras. cardiol ; 107(3): 223-229, Sept. 2016. graf
Article in English | LILACS | ID: lil-796033

ABSTRACT

Abstract Background: Despite the important biological effects of jabuticaba, its actions on the cardiovascular system have not been clarified. Objectives: To determine the effects of jabuticaba hydroalcoholic extract (JHE) on vascular smooth muscle (VSM) of isolated arteries. Methods: Endothelium-denuded aortic rings of rats were mounted in isolated organ bath to record isometric tension. The relaxant effect of JHE and the influence of K+ channels and Ca2+ intra- and extracellular sources on JHE-stimulated response were assessed. Results: Arteries pre-contracted with phenylephrine showed concentration-dependent relaxation (0.380 to 1.92 mg/mL). Treatment with K+ channel blockers (tetraethyl-ammonium, glibenclamide, 4-aminopyridine) hindered relaxation due to JHE. In addition, phenylephrine-stimulated contraction was hindered by previous treatment with JHE. Inhibition of sarcoplasmic reticulum Ca2+ ATPase did not change relaxation due to JHE. In addition, JHE inhibited the contraction caused by Ca2+ influx stimulated by phenylephrine and KCl (75 mM). Conclusion: JHE induces endothelium-independent vasodilation. Activation of K+ channels and inhibition of Ca2+ influx through the membrane are involved in the JHE relaxant effect.


Resumo Fundamentos: Embora a jabuticaba apresente importantes efeitos biológicos, suas ações sobre o sistema cardiovascular ainda não foram esclarecidas. Objetivos: Determinar os efeitos do extrato de jabuticaba (EHJ) sobre o músculo liso vascular (MLV) em artérias isoladas. Métodos: Aortas (sem endotélio) de ratos foram montadas em banho de órgãos isolados para registro de tensão isométrica. Foram verificados o efeito relaxante, a influência dos canais de K+ e das fontes de Ca2+ intra- e extracelular sob a resposta estimulada pelo EHJ. Resultados: Artérias pré-contraídas com fenilefrina apresentaram relaxamento concentração-dependente (0,380 a 1,92 mg/mL). O tratamento com bloqueadores de canais de K+ (tetraetilamônio, glibenclamida, 4-aminopiridina) prejudicaram o relaxamento pelo EHJ. A contração estimulada com fenilefrina também foi prejudicada pelo tratamento prévio com EHJ. A inibição da Ca2+ATPase do reticulo sarcoplasmático não alterou o relaxamento pelo EHJ. Além disso, o EHJ inibiu a contração causada pelo influxo de Ca2+ estimulado por fenilefrina e KCl (75 mM). Conclusão: O EHJ induz vasodilatação independente do endotélio. Ativação dos canais de K+ e inibição do influxo de Ca2+ através da membrana estão envolvidas no efeito relaxante do EHJ.


Subject(s)
Animals , Male , Vasodilator Agents/pharmacology , Plant Extracts/pharmacology , Myrtaceae/chemistry , Muscle, Smooth, Vascular/drug effects , Aorta, Thoracic/drug effects , Time Factors , Vasoconstriction/drug effects , Vasodilation/drug effects , Calcium Channel Blockers/pharmacology , Verapamil/pharmacology , Calcium Channels/drug effects , Potassium Channels/drug effects , Cell Membrane/drug effects , Reproducibility of Results , Rats, Wistar , Potassium Channel Blockers/pharmacology
15.
Rev. cuba. plantas med ; 21(3)jul.-set. 2016. ilus, tab
Article in English | LILACS, CUMED | ID: biblio-845106

ABSTRACT

Introduction: Ugni molinae Turcz., Ugni candollei Barn. (Berg) and Ugni selkirkii (Hook. et Arn.) Berg are Chilean species that share morphological characteristics and they are distributed in geographic locations with very diverse habitats. Its is considered important for the characterization of the Chilean flora to determine if there are similarities in the sort of chemical compounds among species with close morphological relations, growing in different habitats and their consequent biological activity. Objective: to assess the chemical composition and the antioxidant capacity of leaf extracts from the Chilean species of the genus Ugni, and to compare with the U. molinae characteristics. Methods: composition of chemical compounds was determined by chromatographic methods (HPLC-ESI-MS). The antioxidant capacity was assessed by DPPH, ABTS and by stabilization of the hydroxyl radical. Results: as expected, given the great morphological similarity existing among the three species of Ugni that grow in Chile, similarities were found in their chemical composition. Nevertheless, it was also expectable to find variations among them. Thus, U. candollei and U. selkirkii are the species that present greater content and variety of phenolic and terpenic compounds. These species exert greater antioxidant capacity in comparison toU. molinae. U. candollei hightlights for its flavonoid content such as glycosides and quercetin derivatives, and the species U. selkirkii, is important in galotannins. U. molinae is characterized in ellagic acids derivatives. Conclusion: these data and the morphological characteristics could become a useful toll in order to determine the closeness degree among these species(AU


Introducción: Ugni molinae Turcz., Ugni candollei (Barn.) Berg y Ugni selkirkii (Hook. et Arn.) Berg son especies chilenas que comparten características morfológicas y se distribuyen en lugares geográficos con muy diversos hábitats. Se considera importante para la caracterización de la flora chilena determinar si hay similitudes en el tipo de compuestos químicos entre especies con relaciones morfológicas cercanas, que crecen en diferentes hábitats y su actividad biológica consecuente. Objetivo: evaluar la composición química y la capacidad antioxidante de extractos de hojas de las especies chilenas del género Ugni, y compararlas con las características de la especie U. molinae. Métodos: la composición química se determinó por métodos cromatográficos (HPLC-ESI-MS). La capacidad antioxidante se evaluó por los métodos DPPH, ABTS, y la estabilización del radical hidroxilo. Resultados: como era de esperar, dada la gran similitud morfológica existente entre las tres especies de Ugni que crecen en Chile, se encontraron similitudes en su composición química. Sin embargo, también era esperable encontrar variaciones entre ellas. Por lo tanto, U. candollei y U. selkirkii son las especies que presentan mayor contenido y variedad de compuestos fenólicos y terpénicos. Estas especies ejercen una mayor capacidad antioxidante en comparación con U. molinae. U. candollei se caracteriza por su contenido en flavonoides como quercetina glucósidos y sus derivados, y la especie U. selkirkii, por la presencia de galotaninos. U. molinae se caracteriza por contener derivados del ácido elágico. Conclusión: estos datos y las características morfológicas podrían convertirse en una herramienta útil para determinar el grado cercanía entre estas especies(AU)


Subject(s)
Humans , Myrtaceae/chemistry , Plant Preparations/therapeutic use , Antioxidants/therapeutic use , Chile
16.
Bol. latinoam. Caribe plantas med. aromát ; 15(5): 274-287, Sept. 2016. ilus, tab
Article in English | LILACS | ID: biblio-907545

ABSTRACT

The aim of this study was to assess the differences in qualitative-quantitative composition of triterpenoids and total phenolic contents, together with anti-inflammatory activity of Ugni molinae leaves obtained from ten genotypes. The ethyl acetate (EAE) and ethanol extracts (ETE) were obtained and analyzed. The plant genotypes were grown under same soil and climate conditions and under same agronomic management; the leaves were also harvested under the same conditions. Anti-inflammatory activity was evaluated by mice ear edema induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) at a single dose of 200 mg/kg BW of each extract. Composition of triterpenoids and total phenolic contents was determined by HPLC-DAD and Folin-Ciocalteu method, respectively. Ugni molinae leaves of different plant genotypes exhibited significant differences in regard to their anti-inflammatory activity, as well as in qualitative-quantitative composition of triterpenoids and total phenolic content.


El objetivo de este estudio fue establecer las diferencias en la composición cualitativa y cuantitativa de triterpenoides y en los contenidos totales de fenoles, junto con la actividad antiinflamatoria de las hojas de Ugni molinae provenientes de diez genotipos. Los extractos de acetato de etilo (EAE) y etanólicos (ETE) fueron obtenidos y analizados. Los genotipos fueron cultivados bajo las mismas condiciones edafo-climáticas y con el mismo manejo agronómico; las hojas fueron cosechadas bajo las mismas condiciones. La actividad antiinflamatoria fue evaluada en ratones a los que se les indujo un edema en la oreja mediante la aplicación del 12-O-tetradecanoilforbol-13 acetato (TPA) y los extractos fueron evaluados a una dosis única de 200 mg/kg de peso corporal. La composición en triterpenoides y los contenidos de fenoles totales fueron determinados por CLAE-DAD y por el método de Folin-Ciocalteu, respectivamente. Las hojas provenientes de los diferentes genotipos de U. molinae, exhibieron significativas diferencias en sus actividades antiinflamatorias, así como, en el contenido cualitativo y cuantitativo de triterpenoides y en el contenido de fenoles totales.


Subject(s)
Animals , Mice , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/therapeutic use , Edema/drug therapy , Myrtaceae/chemistry , Plant Extracts/chemistry , Triterpenes/analysis , Chromatography, High Pressure Liquid/methods , Plant Extracts/therapeutic use
17.
Rev. bras. plantas med ; 18(2): 502-510, tab, graf
Article in Portuguese | LILACS | ID: lil-787951

ABSTRACT

RESUMO Campomanesia adamantium é muito utilizada na medicina popular no tratamento de hipertensão, reumatismo e diabetes. Objetivou-se com o presente trabalho avaliar o rendimento, a composição química, e as atividades antimicrobiana e antioxidante do óleo essencial de folhas de C. adamantium submetidas a diferentes métodos e tempos de secagem. O delineamento utilizado foi inteiramente casualizado, com oito tratamentos e três repetições. O óleo essencial foi extraído por hidrodestilação das folhas e analisado por CG-EM. O tipo de secagem das folhas de C. adamantium influenciou no teor e na composição química do óleo essencial. Os constituintes espatulenol, óxido cariofileno e germacreno-B apresentaram maior concentração na maioria das procedências avaliadas. O óleo essencial das folhas de C. adamantium apresentou moderada atividade antibacteriana em relação a todos os micro-organismos testados. A atividade antioxidante se deu empregando-se o método de redução do radical estável DPPH, sendo observada fraca atividade antioxidante por meio do ensaio utilizado.


ABSTRACT C. adamantiumis widely used in popular medicine for thetreatment of hypertension, rheumatism and diabetes. This study looked to evaluate the essential oil yield, the chemical composition and the antimicrobial and antioxidant activities of the essential oil from leaves of C. adamantium. A completely randomized design was used with eight treatments and three replications. The essential oil obtained by hydrodestillation of the leaves was analyzed by GC-MS. The results show that the drying type and the processing of the leaf influence the content and chemical composition of the essential oil. The compounds spathulenol, caryophyllene oxide and germacrene-B have higher concentrationsin mostorigins. The essentialoil from theleaves of C.adamantiumshowed moderate bacterial activityagainst all tested microorganisms. The antioxidant activity was determined by the methods of reduction of the DPPH stable radical. The antioxidant activity was weak in the test used.


Subject(s)
Anti-Infective Agents/analysis , Antioxidants/analysis , Myrtaceae/chemistry , Oils, Volatile/analysis , Sesquiterpenes/pharmacology
18.
Rev. bras. plantas med ; 18(1): 19-26, jan.-mar. 2016. tab, graf
Article in Portuguese | LILACS | ID: lil-780042

ABSTRACT

RESUMO A dengue está entre as doenças virais de propagação vetorial mais importante no mundo, causando sérios impactos de morbidade e mortalidade. Desta forma, o presente trabalho teve como objetivo analisar a composição química e a toxicidade do óleo essencial de Myrcia sylvatica (G. Mey) D.C. frente Aedes aegypti e Artemia salina. Folhas de M. sylvatica foram coletadas no Parque Nacional da Chapada das Mesas, no município de Carolina (MA) no mês de fevereiro de 2012. O óleo foi obtido por hidrodestilação e sua composição química foi determinada por cromatografia gasosa acoplada à espectrometria de massa (CG/EM). O bioensaio frente Artemia salina e às larvas de 3° estádio de Aedes aegypti foram realizados em diferentes concentrações. Os dados de mortalidade foram avaliados por regressão linear para determinar os valores de CL50. Obteve-se 0,5% de rendimento, sendo o (E)-cariofileno o constituinte majoritário. O óleo essencial apresentou uma CL50 = 79,44 µg/mL frente A. salina, sendo considerado altamente tóxico. No entanto, este óleo não demonstrou efeito sobre as larvas de A. aegypti. Considerando que o teste de Artemia salina tem correlação com atividades biológicas de grande interesse terapêutico como antitumoral, o óleo essencial das folhas de M. sylvatica demonstrou potencial para desenvolvimento de produtos farmacêuticos.


ABSTRACT Dengue is among the most important viral diseases of vector spread in the world, causing serious impacts of morbidity and mortality. Thus, this study aimed to analyze the chemical composition and toxicity of the Myrcia sylvatica (G. Mey.) DC essential oil against Aedes aegypti and Artemia salina Leach. Leaves of M. sylvatica. were collected in the Tables Chapada National Park, in the municipality of Carolina (MA) in february 2012. The oil was obtained by hydrodistillation and their chemical composition was determined by GC / MS. The bioassay front and Artemia salina larvae of the 3° stage of Aedes aegypti were performed at different concentrations. Mortality data were evaluated by linear regression to determine the LC50. Was obtained 0.5% yield values, and (E) -caryophyllene the major constituent. The essential oil showed a LC50 = 79.44 mg / mL front A. saline and is considered highly toxic. However, this oil showed no effect on larvae of A. aegypti. Whereas the brine shrimp test has regard to several biological activities, as antitumoral property, the essential oil from the leaves of M. sylvatica demonstrated potential for development of medicines.


Subject(s)
Chemistry , Toxicity , Aedes/classification , Myrtaceae/chemistry , Oils, Volatile/chemistry , Artemia/classification , Larvicides
19.
Rev. ciênc. farm. básica apl ; 37(1)2016. ilus, tab
Article in English | LILACS | ID: biblio-964147

ABSTRACT

Camu-camu, a fruit found in the Amazon Basin river banks and lake shores, is known for its high ascorbic acid content together with, other antioxidants. This feature shows high potential for being exploited in agribusiness industry and pharmaceutical processes. However, its high acidity, as well as peel bitterness associated with the phenolic substances content, has discouraged its consumption while still fresh. The development of alternative forms for consuming this fruit, while still preserving its ascorbic acid and polyphenol content, in addition to its great potential for maintaining human health, has become a major economic activity in coastal communities. The present study evaluated the ascorbic acid stability found in camu-camu capsules. Lyophilization was performed with the fruit pulp and peel. Both freeze-dried fruit and powder-flled capsules were stored at 5° C. Ascorbic acid stability was monitored for 90 days using HPLC assay technique. The encapsulation process of freezedried pulp was considered satisfactory in the ascorbic acid conservation, since there was only a loss of 10% of its initial concentration throughout the study period for 60 days.(AU)


Subject(s)
Humans , Ascorbic Acid/analogs & derivatives , Capsules/chemistry , Myrtaceae/chemistry , Fruit , Brazil , Freeze Drying/methods , Fruit/chemistry , Antioxidants
20.
Braz. j. biol ; 75(4): 948-952, Nov. 2015. tab, graf
Article in English | LILACS | ID: lil-768200

ABSTRACT

Abstract There is considerable interest in identifying new antioxidants from plant materials. Several studies have emphasized the antioxidant activity of species belonging to the Myrtaceae family. However, there are few reports on these species from the Cerrado (Brazilian savanna). In this study, the antioxidant activity and phenolic content of 12 native Myrtaceae species from the Cerrado were evaluated (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum, and Psidium laruotteanum). Antioxidant potential was assessed using the antioxidant activity index (AAI) by the DPPH method and total phenolic content (TPC) by the Folin–Ciocalteu assay. There was a high correlation between TPC and AAI values. Psidium laruotteanum showed the highest TPC (576.56 mg GAE/g extract) and was the most potent antioxidant (AAI = 7.97, IC50 = 3.86 µg·mL−1), with activity close to that of pure quercetin (IC50 = 2.99 µg·mL−1). The extracts of nine species showed IC50 of 6.24–8.75 µg·mL−1. Most species showed TPC and AAI values similar to or higher than those for Camellia sinensis, a commonly consumed tea with strong antioxidant properties. The results reveal that the analyzed Myrtaceae species from the Cerrado possess high phenolic contents and antioxidant activities. Thus, they are a potential source of new natural antioxidants.


Resumo Há um considerável interesse na descoberta de novos antioxidantes de origem vegetal. Muitos estudos enfatizaram a atividade antioxidante de espécies pertencentes à família Myrtaceae. No entanto, há poucos relatos sobre espécies do Cerrado. Neste estudo, a atividade antioxidante e o conteúdo fenólico de 12 espécies nativas de Myrtaceae do Cerrado foram avaliados (Blepharocalyx salicifolius, Eugenia bimarginata, Eugenia dysenterica, Eugenia klotzschiana, Hexachlamys edulis, Myrcia bella, Myrcia lingua, Myrcia splendens, Myrcia tomentosa, Psidium australe, Psidium cinereum e Psidium laruotteanum). O potencial antioxidante foi estimado através do índice de atividade antioxidante (AAI) pelo método do DPPH e o conteúdo fenólico total (TPC) pelo ensaio de Folin-Ciocaulteu. Houve uma alta correlação entre os valores de TPC e AAI. P. laruotteanum teve o maior TPC (576,56 mg de equivalente em ácido gálico por g de extrato) e foi o antioxidante mais potente (AAI = 7,97, IC50 = 3,86 µg.mL–1), com atividade próxima da quercetina pura (IC50 = 2,99 µg.mL–1). Os extratos de nove espécies apresentaram IC50 de 6,24 a 8,75 µg.mL–1. Além disso, a maioria das espécies teve valores de TPC e AAI similares ou maiores que Camellia sinensis, cujo chá é comumente consumido e apresenta fortes propriedades antioxidantes. Os resultados mostraram que as espécies de Myrtaceae do Cerrado analisadas apresentam conteúdos fenólicos e atividades antioxidantes elevadas. Dessa forma, elas são uma fonte potencial de novos antioxidantes.


Subject(s)
Antioxidants/metabolism , Myrtaceae/chemistry , Phenols/metabolism , Plant Extracts/chemistry , Brazil , Grassland , Plant Leaves/chemistry
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