Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 8 de 8
Filter
1.
J. coloproctol. (Rio J., Impr.) ; 41(4): 393-405, Out.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1356431

ABSTRACT

Background: Anatomopathological staging is the primary method to determine the prognosis of patients with colorectal carcinoma (CRC). However, new tools have been developed that can complement it, such as the analysis of the elevation of systemic inflammatory markers. Objective: To evaluate the impact of the elevation of scores based on inflammatory markers (the neutrophil-to-lymphocyte ratio [NLR], the Glasgow Prognostic Score [GPS], and isolated C-reactive protein [CRP]) in the prognosis of patients diagnosed with CRC and submitted to potentially curative surgery in Hospital de Braga, Portugal, between January 1st, 2005, and December 31st, 2010. Methods: A retrospective analysis of the data of 426 patients was performed, with a collection of several clinico-pathological variables, as well as the levels of lymphocytes, neutrophils, albumin and CRP, in the pre- and postoperative periods, to apply the different scores to the sample. Results: From the analysis of the survival curves, we concluded that patients with increased NLR in the pre- and postoperative periods present a lower cancer-related survival than patients with normal NLR (preoperative period: 93.7 versus 122 months; p<0.001; postoperative period: 112 versus 131 months; p=0.002). Patients with increased NLR in the pre- and postoperative periods also had a lower disease-free survival (preoperative period: 88.0 versus 122 months; p<0.001; postoperative period: 111 versus 132 months; p=0.002). In addition, increased pre- and postoperative NLR was associatedwith a higher risk of death due to CRC (preoperatively: hazard ratio [HR]=2.25; p<0.001; postoperatively: HR=2.18; p=0.003). However, the multivariate analysis shows that only postoperative NLR (ajusted HR =2.66; p=0.002) does so independently of the remaining variables. Conclusion: Regarding the scores applied to the sample, the NLR was the one that most consistently related to the prognosis of the patients. However, it would be useful to develop a prospective study that could confirm this relationship. (AU)


Subject(s)
Humans , Male , Female , Prognosis , Colorectal Neoplasms/mortality , C-Reactive Protein/analysis , Colorectal Neoplasms/therapy , Survival Rate , Disease-Free Survival , NLR Proteins/analysis
2.
Hematol., Transfus. Cell Ther. (Impr.) ; 43(4): 424-429, Oct.-Dec. 2021. tab, graf
Article in English | LILACS | ID: biblio-1350803

ABSTRACT

ABSTRACT Introduction: Smoking is associated with the occurrence and progression of cardiovascular diseases, inflammatory disorders and malignancies. Objective: To study the platelet indices, neutrophil lymphocyte ratio (NLR) and platelet lymphocyte ratio (PLR) in smokers and their correlation with smoking pack-years. Method: A total of 110 smokers and 110 non-smokers were included. The smokers were grouped into three groups: mild (<5 pack-years), moderate (5-10 pack-years) and heavy (>10 pack-years). The platelet count, plateletcrit (PCT), mean platelet volume (MPV) and platelet distribution width (PDW) were noted. The NLR and PLR were calculated and the statistical analysis was made using the Student's T-test, Analysis of Variance (ANOVA) and Spearman's correlation coefficient. Results: The platelet count, PCT and PDW were significantly higher with mean values: 218.56 ± 121.31 vs 203.23 ± 80.35 (p-value = 0.038), 0.27 ± 0.10 vs 0.26 ± 0.10 (p-value = 0.041) and 12.54 ± 1.45 vs 11.99 ± 1.70 (p-value = 0.001) in smokers and non-smokers, respectively. The PLR differed significantly with mean values: 119.40 ± 84.81 in smokers and 181.99 ± 313.09 in non-smokers, with a p-value of 0.045. A significant positive correlation was found between pack-years of smoking and platelet count and PLR with the Pearson correlation coefficient of 0.250 and 0.198 and p-values, 0.008 and 0.037, respectively. The Platelet Count, PCT, MPV and PDW varied significantly between mild, moderate and heavy smoker groups, with p-values of 0.045, 0.010, 0.015 and 0.017, respectively. Conclusion: The platelet indices and inflammatory markers NLR and PLR are derived from routine blood investigations, which are easily available and inexpensive. The monitoring of platelet indices, along with the PLR, can be used as early predictors of morbidity in smokers.


Subject(s)
Humans , Male , Female , Tobacco Use Disorder , Mean Platelet Volume , Thromboembolism , Lymphocytes , NLR Proteins , Heart Disease Risk Factors , Neutrophils
3.
Article in Chinese | WPRIM | ID: wpr-879867

ABSTRACT

OBJECTIVE@#To study the effect of astragaloside IV (AS-IV) on NOD-like receptor protein 3 (NLRP3) inflammasome in neonatal rats with hypoxic-ischemic brain damage (HIBD).@*METHODS@#A total of 24 Sprague-Dawley rats, aged 7 days, were randomly divided into a sham-operation group, an HIBD group, and an AS-IV treatment group, with 8 rats in each group. After 24 hours of modeling, brain tissue was collected for hematoxylin-eosin staining, yo-PRO-1 staining, and EthD-2 immunofluorescent staining in order to observe the cerebral protection effect of AS-IV in vivo. HT22 cells were used to prepare a model of oxygen-glycogen deprivation (OGD), and a concentration gradient (50-400 μmol/L) was established for AS-IV. CCK-8 assay was used to measure the viability of HT22 cells. RT-PCR and Western blot were used to observe the effect of different concentrations of AS-IV on the mRNA and protein expression of NLRP3, gasdermin D (GSDMD), caspase-1, and interleukin-1β (IL-1β).@*RESULTS@#Yo-Pro-1 and EthD-2 staining showed that compared with the sham-operation group, the HIBD group had an increase in pyroptotic cells with a small number of necrotic cells, and the AS-IV group had reductions in both pyroptotic and necrotic cells. Compared with the sham-operation group, the HIBD group had significantly higher protein expression levels of NLRP3, IL-1β, caspase-1, and GSDMD (@*CONCLUSIONS@#AS-IV may alleviate HIBD in neonatal rats by inhibiting the expression of NLRP3, GSDMD, caspase-1, and IL-1β.


Subject(s)
Animals , Animals, Newborn , Brain , Hypoxia-Ischemia, Brain/drug therapy , Inflammasomes , NLR Proteins , Rats , Rats, Sprague-Dawley , Saponins , Triterpenes
5.
Chinese Medical Journal ; (24): 20-27, 2020.
Article in English | WPRIM | ID: wpr-878002

ABSTRACT

The NOD-like receptor protein 3 (NLRP3) inflammasome is a key regulator of the host's immune response, and many immune and metabolic disorders are linked to its activation. This review aimed to investigate and clarify the relationship between this inflammasome and high-risk reproductive disorders. Papers cited here were retrieved from PubMed up to August 2020 using the keywords "NLRP3" or "NALP3", "caspase-1", "endometriosis", "gestational diabetes", "interleukin (IL)-18", "IL-1β", "pre-eclampsia (PE)", "preterm birth", "polycystic ovarian syndrome (PCOS)", "recurrent spontaneous abortion (RSA)", and combinations of these terms. The results show that NLRP3 inflammasome is associated with various high-risk reproductive disorders and many inflammatory factors are secreted during its activation, such as IL-1β induced during the development of endometriosis. PCOS is also associated with activation of the NLRP3 inflammasome, especially in overweight patients. It also participates in the pathogenesis of RSA and is activated in fetal membranes before preterm birth. The placentas of pregnant women with PE show higher expression of the NLRP3 inflammasome, and gestational diabetes mellitus occurs simultaneously with its activation. Current evidence suggest that the NLRP3 inflammasome plays an important role in female reproductive disorders. New treatment and management methods targeting it might help reduce the incidence of such disorders and improve neonatal outcomes.


Subject(s)
Caspase 1 , Female , Humans , Infant, Newborn , Inflammasomes , Interleukin-1beta , NLR Family, Pyrin Domain-Containing 3 Protein , NLR Proteins , Pregnancy , Premature Birth , Risk Assessment
6.
Article in Chinese | WPRIM | ID: wpr-819080

ABSTRACT

The innate immune system provides a first line of defense against invading pathogens, in which the pattern recognition receptors (PRR) recognize pathogen-associated molecular patterns (PAMP) and initiate the downstream signaling pathways to eliminate the encountered pathogens. There are two main classes of such signaling pathways: NOD-like receptor (NLR) signaling pathway and Toll-like receptor (TLR) signaling pathway. The microbial pathogens under selective pressure have evolved numerous mechanisms to avoid and/or manipulate the NLR and TLR signal transduction for survival and replication. To evade the NLR signaling pathway, pathogens interfere and/or inhibit inflammasome activation in innate immune cells by producing virulence factors or reducing PAMPs expression. The mechanisms for pathogens to evade TLR signaling pathway include: inhibition of mitogen activated protein kinases (MAPKs) cascade reaction, inhibition of NF-КB activation, and interference of down-stream signal transduction by producing Toll/interleukin-1 receptor (TIR)-containing proteins which bind directly with TLRs or adaptor proteins in the signaling pathway.


Subject(s)
Immunity, Innate , NLR Proteins , Allergy and Immunology , Receptors, Interleukin-1 , Metabolism , Signal Transduction , Toll-Like Receptors , Allergy and Immunology
7.
Article in Chinese | WPRIM | ID: wpr-317739

ABSTRACT

<p><b>OBJECTIVE</b>This study aims to investigate the regulatory effects of calcitonin gene-related peptide (CGRP) on Nod-like receptor protein 3 (NLRP3) and interleukin-1β (IL-1β) to promote osteoblast differentiation.</p><p><b>METHODS</b>Different concentrations of CGRP (0, 10, 30, 100 ng · mL⁻¹) were added to mouse osteoblasts in vitro. The mRNA and protein expression levels of both NLRP3 and IL-1β were examined using Real-time polymerase chain reaction and Western blot, respectively. Moreover, the concentrations of IL-1β protein and intracellular reactive oxygen species (ROS) were detected using enzyme-linked immunosorbent assay and flow cytometry, respectively. The osteogenic differentiation of mouse osteoblasts was identified through alizarin red staining.</p><p><b>RESULTS</b>The protein and mRNA expression levels of both NLRP3 and IL-1β significantly decreased (P < 0.05) with increasing CGRP concentration. Moreover, the contents of intracellular ROS gradually decreased (P<0.05). The osteogenic differentiation of the osteoblasts was more enhanced in the group treated with 100 ng · mL⁻¹ CGRP than in the empty group (0 ng · mL⁻¹ CGRP).</p><p><b>CONCLUSION</b>CGRP promotes osteoblast differentiation by inhibiting the expression of inflammatory factors.</p>


Subject(s)
Animals , Blotting, Western , Calcitonin , Calcitonin Gene-Related Peptide , Cell Differentiation , Enzyme-Linked Immunosorbent Assay , Interleukin-1beta , Mice , NLR Family, Pyrin Domain-Containing 3 Protein , NLR Proteins , Osteoblasts , Osteogenesis , RNA, Messenger , Reactive Oxygen Species , Real-Time Polymerase Chain Reaction
8.
Iatreia ; 27(2): 135-146, Apr.-June 2014. ilus, tab
Article in English | LILACS, COLNAL | ID: lil-712464

ABSTRACT

Objetivo: evaluar la expresión y la función de receptores de reconocimiento de patrones como los de tipo Toll y los de tipo NOD, RIG-I/MDA5, la dectina-1 y moléculas adaptadoras, en neutrófilos humanos. Métodos: a partir de sangre periférica de individuos sanos se purificaron y cultivaron neutrófilos en el medio RMPI-1640, en presencia o ausencia de los agonistas específicos de los receptores de interés. La expresión de los receptores de reconocimiento de patrones se determinó por RT-PCR y la secreción de citocinas proinflamatorias, por ELISA. Resultados: los neutrófilos expresan un amplio espectro de receptores de reconocimiento de patrones y de moléculas adaptadoras. La estimulación de TLR4, TLR5, TLR7/8 induce la secreción de IL-1β e IL-6; la activación de la dectina-1 induce una alta producción de TNF-α, pero bajos niveles de IL-1β e IL-6. Conclusión: los neutrófilos expresan un amplio número de receptores de reconocimiento de patrones y su activación lleva a la expresión de diferentes citocinas proinflamatorias.


Objective: To evaluate the expression and function of pattern recognition receptors such as Toll-like receptors, RIG-I/MDA5, NOD-like receptors, Dectin-1 and adaptor proteins, in human neutrophils. Methods: Neutrophils from peripheral blood of healthy individuals were purified and cultured in RPMI-1640, in the presence or absence of specific agonists of the receptor of interest. The expression of pattern recognition receptors was determined by RT-PCR and the secretion of proinflammatory cytokines, by ELISA. Results: We observed that neutrophils express diverse patterns recognition receptors and adaptor molecules. Stimulation of TLR4, TLR5 and TLR7/8 induces the production of IL-1β and IL-6, and activation of Dectin-1 leads to secretion of high levels of TNF-α, but low levels of IL-1β and IL-6. Conclusion: Neutrophils express a large number of pattern recognition receptors and their activation leads to the expression of proinflammatory cytokines.


Subject(s)
Humans , Cytokines , Neutrophils , NLR Proteins , Receptors, Pattern Recognition , Toll-Like Receptors
SELECTION OF CITATIONS
SEARCH DETAIL