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1.
Braz. j. microbiol ; 47(3): 551-562, July-Sept. 2016. tab, graf
Article in English | LILACS | ID: lil-788953

ABSTRACT

ABSTRACT The goal of this investigation was to isolate competent polynuclear aromatic hydrocarbons degraders that can utilize polynuclear aromatic hydrocarbons of former industrial sites at McDoel Switchyard in Bloomington, Indiana. Using conventional enrichment method based on soil slurry, we isolated, screened and purified two bacterial species strains PB1 and PB2. Applying the ribotyping technique using the 16S rRNA gene analysis, the strains were assigned to the genus Pseudomonas (Pseudomonas plecoglossicida strain PB1 and Pseudomonas sp. PB2). Both isolates showed promising metabolic capacity on pyrene sprayed MS agar plates during the preliminary investigations. Using time course studies in the liquid cultures at calculated concentrations 123, 64, 97 and 94 ppm for naphthalene, chrysene, fluroanthene and pyrene, P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 showed partial utilization of the polynuclear aromatic hydrocarbons. Naphthalene was degraded between 26% and 40%, chrysene 14% and 16%, fluroanthene 5% and 7%; pyrene 8% and 13% by P. plecoglossicida strain PB1 and Pseudomonas sp. PB2 respectively. Based on their growth profile, we developed a model R2 = 1 to predict the degradation rate of slow polynuclear aromatic hydrocarbon-degraders where all the necessary parameters are constant. From this investigation, we confirm that the former industrial site soil microbial communities may be explored for the biorestoration of the industrial site.


Subject(s)
Polycyclic Aromatic Hydrocarbons/metabolism , Pseudomonas/metabolism , Phylogeny , Pseudomonas/classification , Pseudomonas/genetics , Pyrenes/metabolism , Soil/chemistry , Soil Microbiology , Biodegradation, Environmental , Carbon/chemistry , RNA, Ribosomal, 16S/genetics , Chrysenes/metabolism , Naphthalenes/metabolism , Nitrogen/chemistry
2.
J Biosci ; 2006 Jun; 31(2): 219-22
Article in English | IMSEAR | ID: sea-110864

ABSTRACT

A sensitive,specific, and rapid method for the detection of carbohydrate-protein interactions is demonstrated by fluorophore-assisted carbohydrate electrophoresis (FACE). The procedure is simple and the cost is low. The advantage of this method is that carbohydrate-protein interactions can be easily displayed by FACE, and the carbohydrates do not need to be purified.


Subject(s)
Carbohydrates , Electrophoresis/economics , Fluorescent Dyes/metabolism , Naphthalenes/metabolism , Proteins/metabolism , Sensitivity and Specificity
3.
Indian J Exp Biol ; 2004 Feb; 42(2): 214-6
Article in English | IMSEAR | ID: sea-56585

ABSTRACT

Esterase activity of resistant and susceptible H. armigera were compared in gels with different substrate such as naphthyl acetate, naphthyl phosphate, paraoxon and monocrotophos. Whole body extract of resistant H. armigera hydrolyzed paraoxon, monocrotophos and naphthyl phosphate in gels. Resistant H. armigera showed high esterase, phosphatase and paraoxon hydrolase activity compared to susceptible ones.


Subject(s)
Animals , Esterases/metabolism , Hydrolysis , Insecticide Resistance , Insecticides/metabolism , Larva/drug effects , Lepidoptera/metabolism , Monocrotophos/metabolism , Naphthalenes/metabolism , Naphthols/metabolism , Organophosphorus Compounds/metabolism , Paraoxon/metabolism , Phosphoric Monoester Hydrolases/metabolism
4.
Indian J Exp Biol ; 1995 May; 33(5): 353-6
Article in English | IMSEAR | ID: sea-56751

ABSTRACT

A Pseudomonas strain NGK1 capable of degrading naphthalene as a sole carbon source was isolated from biological waste treatment effluent. After 48 hr of incubation in 0.1% naphthalene mineral salts medium, the bacterial culture showed irregular clumped cells and salicylic acid (68 micrograms per ml) in the medium (pH 3.8). The strain degraded naphthalene through salicylate and catechol as was evidenced by metabolite characterization, oxygen uptake and enzymatic studies.


Subject(s)
Naphthalenes/metabolism , Pseudomonas/metabolism
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