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China Journal of Chinese Materia Medica ; (24): 4647-4654, 2023.
Article in Chinese | WPRIM | ID: wpr-1008631


To explore the suitable fertilizing pattern for Saposhnikovia divaricata in the genuine producing area, a field trial was carried out to investigate the changes in the yield and quality of medicinal materials and soil in different fertilization patterns, such as organic fertilizer substitution(organic fertilizer+NPK fertilizer) and chemical fertilizer reduction(organic fertilizer+NPK fertilizer decrement and organic fertilizer+NPK fertilizer decrement+soil conditioner). The comprehensive analysis of all treatments was based on the medicine quality evaluation data set and soil quality evaluation data set, respectively, by CRITIC weight method. The results showed that(1) the yield of S. divaricate increased by 4.93%-12.67% under the organic fertilizer substitution mode, and the yield increased by 44.43% under the treatment of chemical fertilizer reduction YHT15, which was higher than that of the organic fertilizer substitution mode.(2) The quality of S. divaricate under the two fertilization modes was superior to the standard in the Chinese Pharmacopoeia, and the application of biochar was helpful to improve the quality of S. divaricate quality, with an increase of 82.83%-181.54%. CRITIC method analysis showed that fertilization treatments with high comprehensive scores were YHT15, YH30, and YH15.(3) Soil quality under the two fertilization modes was higher than that under the control. The fertilization treatments with higher comprehensive scores of soil quality were YHT15, YHT30, and YHT. The fertilization mode of adding biochar as soil conditioner, applying an appropriate amount of organic fertilizer, and reducing part of chemical fertilizer is the appropriate way to develop ecological plantation of S. divaricata in the Baicheng area in the western Jilin province. The specific fertilization mode is as follows. The basic fertilizer was 361 kg·hm~(-2) superphosphate+110 kg·hm~(-2) potassium sulfate+82 kg·hm~(-2) organic fertilizer+10 000 kg·hm~(-2) rice husk biochar, and urea was applied as top fertilizer three times, 29, 29, and 20 kg·hm~(-2), respectively.

Agriculture , Fertilizers/analysis , Soil , Apiaceae , Nitrogen/analysis
Cienc. tecnol. salud ; 9(1): 6-18, 2022. il^c27
Article in Spanish | LILACS, DIGIUSAC, LIGCSA | ID: biblio-1382357


La deposición de nutrientes por vía atmosférica tiene graves impactos sobre la ecología de bosques y cuerpos de agua templados. Sin embargo, su importancia en cuerpos de agua neotropicales casi no ha sido estudiada. En este artículo se cuantifica la contribución de nitrógeno inorgánico disuelto (NID, [NO3--N + NH4+-N]) y fósforo inorgánico soluble (FIS, [PO4-3-P]) depositados en bulto sobre superficies húmedas por vía atmosférica hacia el lago Atitlán (Guatemala). Las cargas estimadas de NID y FIS consecuencia de la deposición atmosférica directa (depositada sobre la superficie del lago) fueron de 151.2 ton/año y 5.6 ton/año, respectivamente. Con estos resulta-dos, se estima que el aporte de FIS por deposición atmosférica al lago Atitlán es comparable al de sus principales ríos tributarios, y de casi el doble para el ingreso de NID. Las estimaciones para el lago Atitlán son mayores que lo reportado para otros lagos. Nuestro estudio proporciona información básica para entender la eutrofización del lago Atitlán, enfatizado en la importancia de la deposición atmosférica como contribuyente al deterioro de este cuerpo de agua. Además, demuestra la necesidad de extender este tipo de estudio a otras cuencas neotropicales y la importancia de minimizar este impacto.

Atmospheric nutrient deposition has serious impacts on the ecology of forests and temperate water bodies nevertheless its importance in Neotropical water bodies has hardly been studied. Here we quantify the contribution of bulk atmospheric deposition on wet surfaces of dissolved inorganic nitrogen (DIN,[NO3--N + NH4+-N])and soluble inorganic phosphorus (SIP, [PO4-3-P])into Lake Atitlán (Guatemala). The estimated NID and SIP loads from this direct deposition on the lake surface were respectively, 151.2 tons/year and 5.6 tons/year. With these results, we estimated that the SIP input from atmospheric deposition to Lake Atitlán is comparable to that from the lake's main tributary rivers, whereas for DIN entry this is almost twice as much. Estimates for Lake Atitlán are higher than those reported for many lakes. Our study provides basic information towards understanding the eutrophication of Lake Atitlán, emphasizes the importance of atmospheric deposition in this process and the need for additional studies to document the process in neotropical watersheds.

Humans , Phosphorus/analysis , Lakes/analysis , Nitrogen/analysis , Rain/chemistry , Temperature , Wind , Basins , Nutrients , Eutrophication
Braz. j. biol ; 82: 1-9, 2022. ilus, tab, graf
Article in English | LILACS, VETINDEX | ID: biblio-1468472


Root deformation (RD) caused by errors in the pricking out process are irreversible and very difficult to detect in container-grown seedlings at the time of planting in the field. The objective of this study was to evaluate the effects of RD on leaf gas exchange, growth, biomass allocation and mineral nutrition of G. americana seedlings during the recovery phase after soil flooding. Four-months-old seedlings, with and without RD, were flooded for 42 days and their recovery was evaluated 28 days after soil drainage. There were no significant interactions between RD and soil flooding for all leaf gas exchange, growth and mineral nutrition after soil drainage, with the exception of leaf P concentrations. In plants with no RD, the P concentration in leaves of non-flooded plants was significantly higher than that of plants with RD. Soil flooding and RD did not influence leaf or root N concentrations or whole-plant N content. RD increased the K concentration in the roots, but not in the leaves. Changes in the nutrient concentrations in leaves and roots indicate that RD may affect physiological performance of seedlings after planting in the field.

A deformação da raiz (RD) causada por erros no processo de repicagem é irreversível e difícil de detectar em mudas produzidas em embalagens no momento do plantio no campo. O objetivo deste estudo foi avaliar os efeitos do RD nas trocas gasosas foliares, crescimento, alocação de biomassa e nutrição mineral de mudas de G. americana na fase de recuperação após o alagamento do solo. Mudas com quatro meses de idade, com e sem RD, foram alagadas por 42 dias e a sua recuperação foi avaliada 28 dias após a drenagem do solo. Não houve interação significativa entre RD e alagamento do solo nas trocas gasosas foliares, crescimento e nutrição mineral após a drenagem, com exceção das concentrações de P foliar. Em plantas sem RD, a concentração de P nas folhas de plantas não alagadas foi significativamente maior que a das plantas com RD. O alagamento do solo e a RD não influenciaram as concentrações de N nas folhas e raízes, e no conteúdo de N na planta inteira. A RD aumentou a concentração de K nas raízes, mas não nas folhas. Alterações nas concentrações de nutrientes nas folhas e raízes indicam que a RD pode afetar o desempenho fisiológico das mudas após o plantio no campo.

Phosphorus/analysis , Nitrogen/analysis , Nutrients/analysis , Potassium/analysis , Plant Roots/growth & development , Rubiaceae/growth & development , Rubiaceae/physiology , Soil Moisture
Biosci. j. (Online) ; 37: e37013, Jan.-Dec. 2021. graf
Article in English | LILACS | ID: biblio-1358706


Nitrogen (N) is an essential macronutrient for plant growth and rate applications can influence the performance of sesame, and when applied in excess can cause nitrogen loss in the environment, and consequently make the cost of production more costly to the producer. Therefore, the objective of this work was to evaluate the efficiency of nitrogen use by different cultivars of irrigated sesame seeds under the edaphoclimatic conditions of the northeastern semi-arid region in two harvests. The experiments were carried out from February to May (1st harvest) and from July to October (2nd harvest) in 2016. The treatments were arranged in a split plot scheme, in which the plots were the five nitrogen doses (0, 30, 60, 90 and 120 kg ha-1), and in the subplots, the four sesame genotypes (CNPA G2, CNPA G3, CNPA G4 and BRS Seda), the design was in randomized complete blocks with four replications. The nitrogen use efficiency assessments evaluated were: agronomic efficiency (AE), physiological efficiency (PE), agrophysiological efficiency (APE), recovery efficiency (RE) and efficiency of use (EU). The rate that provided the greatest efficiency of use was 30 kg ha-1 of N applied. The cultivar BRS Seda had greater efficiency of use in relation to the other cultivars studied. The crop that had better efficiency of use was the 2nd agricultural harvest.

Crop Production , Sesamum/genetics , Nitrogen/analysis
China Journal of Chinese Materia Medica ; (24): 1883-1892, 2021.
Article in Chinese | WPRIM | ID: wpr-879106


Nitrogen fertilizer has been the long-lasting crucial component in cultivation of Chinese materia medica(CMM) and crops for its profound effects on enhancing the productivity. In consideration of its role in better production, intensive and excessive application of N fertilizer is often found in CMM cultivation. Therefore, firstly, this review summarized various concentrations of N application with regards to different CMM and districts from the literatures published in the last two decades. The recommended concentration of nitrogen application of forty seven CMM species were covered in this review. We found that the optimum rates of nitrogen fertili-zer for different medicinal plants species were varied in the range between 0-1 035.55 kg·hm~(-2). Most of the optimum rates of nitrogen fertilizer for CMM in published researches fell between 100-199 kg·hm~(-2). The optimum rate of nitrogen fertilizer is not only related to amount of nitrogen required for different medicinal plants but also to soil fertilities of different fields. In addition, we outlined the diffe-rent effects of proper and excessive nitrogen deposition on yield of CMM. Proper nitrogen deposition benefits the yield of CMM, howe-ver, excessive nitrogen use accounts for a decrease in CMM yield. We elucidated that nutritional content, water use efficiencies, and photosynthesis capacity were major influencing factors. Researches showed that proper nitrogen fertilizer could promote the water use efficiencies of plants and boost photosynthesis. Consequently, the yield of CMM can be enhanced after nitrogen deposition. However, negative effects of nitrogen fertilizer were also found on plant including producing toxic substances to the soil and causing severe pest damages. Lastly we analyzed the impact of N fertilizer application on secondary metabolites which accounts for a large part of active pharmaceutical ingredients of CMM. It usually caused an increase in nitrogen-containing secondary metabolites content and a decrease in non-nitrogen-containing secondary metabolites content respectively. The potential underlying mechanisms are the different synthetic pathways of these metabolites and the plant nutritional status. Synthesis of non-nitrogen-containing secondary metabolites like phenols can be inhibited after nitrogen application because of the competition of the same precursor substances between metabolites synthesis and plant growth. To sum up, impacts and mechanisms of nitrogen fertilizer on yield and quality enhancement of CMM were discussed in this review. Negative effects of excessive nitrogen application on CMM should be paid special attention in CMM cultivation and prescription fertilization based on the field soil quality is strongly recommended. Overall, this review aims to provides insights on improving the proper application of N fertilizer in the cultivation of CMM.

Agriculture , China , Fertilizers , Materia Medica , Nitrogen/analysis , Soil
Biosci. j. (Online) ; 36(Supplement1): 217-227, Dec. 2020. ilus, tab, graf
Article in English | LILACS | ID: biblio-1355250


Nitrogen accumulation in hydroponically-grown lettuce may pose a health risk to consumers. Thus, the objective of this study was to analyze different concentrations of nitrogen applications in hydroponic lettuce cultivation and their effect on toxicity, cytotoxicity and genotoxicity. A nutrient film technique (NFT) hydroponic system was used to grow the lettuce variety "Vanda." The treatments consisted of different concentrations of nitrogen (in the form of calcium nitrate) in Furlani solution (75, 100, 125 and 150%), a negative and a positive control. The following commercial characteristics were measured: plant fresh weight (PFW), root fresh weight (RFW), shoot fresh weight (SFW), shoot diameter (SD), root dry weight (RDW), shoot dry weight (SDW) and leaf nitrogen (LN). Cytogenotoxicity was indicated by toxicity, cytotoxicity and genotoxicity, which were in turn determined by root length, the mitotic index, chromosomal aberrations and the presence of micronuclei. The nitrogen concentrations used in this experiment did not cause phenotypic toxicity or cytotoxicity in lettuce roots. The most severe genotoxicity was observed at the 125% nitrogen concentration, which nevertheless did not affect commercial characteristics. Although nitrogen fertilization provides great benefits to agriculture, such as greater yields, indiscriminate use should be avoided since concentrations above recommended rates may induce genotoxicity.

O acúmulo de nitrogênio em alface cultivada hidroponicamente pode representar um risco à saúde dos consumidores. Assim, o objetivo deste estudo foi analisar diferentes concentrações de aplicações de nitrogênio no cultivo de alface hidropônica e seus efeitos na toxicidade, citotoxicidade e genotoxicidade. Em sistema hidropônico do tipo filme de nutrientes (NFT) foi usado para cultivar a variedade de alface "Vanda". Os tratamentos consistiram em diferentes concentrações de nitrogênio (na forma de nitrato de cálcio) na solução Furlani (75, 100, 125 e 150%), um controle negativo e um positivo. Foram medidas as seguintes características comerciais: peso fresco da planta (PFW), peso fresco da raiz (RFW), peso fresco da parte aérea (SFW), diâmetro da parte aérea (SD), peso seco da raiz (RDW), peso seco da parte aérea (SDW) e nitrogênio foliar (LN). A citogenotoxicidade foi indicada por toxicidade, citotoxicidade e genotoxicidade, que por sua vez foram determinadas pelo comprimento da raiz, índice mitótico, aberrações cromossômicas e presença de micronúcleos. As concentrações de nitrogênio utilizadas neste experimento não causaram toxicidade fenotípica ou citotoxicidade em raízes de alface. A genotoxicidade mais severa foi observada na concentração de nitrogênio de 125%, que, no entanto, não afetou as características comerciais. Embora a fertilização nitrogenada traga grandes benefícios à agricultura, como maiores rendimentos, o uso indiscriminado deve ser evitado, pois concentrações acima das taxas recomendadas podem induzir genotoxicidade.

Lactuca , Toxicity , Genotoxicity , Nitrogen/analysis
Cienc. tecnol. salud ; 7(2): 155-169, 2020. il 27 c
Article in Spanish | LILACS, DIGIUSAC, LIGCSA | ID: biblio-1348112


Se realizó un estudio sobre la composición y abundancia del fitoplancton en el lago de Amatitlán, y el efecto de la calidad de agua sobre su biodiversidad. Para ello, se colectaron muestras de agua en cuatro puntos específicos del lago, en la superficie del agua y a profundidades de 5, 10 y 20 m, de manera mensual durante el 2017. Se midieron parámetros fisicoquímicos in situ como temperatura y pH. Igualmente, se identificaron y contabilizaron cianobacterias y microalgas. El índice de estado trófico (IETP) catalogó al lago como eutrófico e hipertrófico (IETP = 63.80-88.18). Se reportan 34 géneros de fitoplancton distribuidos en 30 familias, 17 órdenes y 10 clases. Los indicadores biológicos, tales como, floraciones algales de Microcystis (38.41%), baja diversidad de diatomeas (Nitzschia, Aulacoseira y Cyclotella), presencia de microalgas Nitzschia y Scenedesmusresistentes a procesos de eutrofización, y alta concentración de coliformes fecales, de hasta 24,000 NMP/100 ml, evidenciaron la baja calidad de agua que se presenta en el lago de Amatitlán. En época seca se encontró más diversidad de microalgas debido a la mayor incidencia de radiación solar, el poco recambio de agua y la acumulación de materia orgánica. Aunque esto varía con los cambios en la concentración de nitrógeno total (NT) y fosforo total (PT), que potencian la proliferación de cianobacterias tóxicas. La biodiversidad del lago fue baja debido al estado hipereutrófico en que se encuentra. Se recomienda poner en funcionamiento plantas de tratamiento de aguas residuales para evitar que esta problemática continúe.

A study was carried out on the composition and abundance of phytoplankton in Amatitlán lake, and the effect of water quality on its biodiversity. For this, water samples were collected at four specific points in the lake, on the water surface and at depths of 5, 10 and 20 m, in a monthly way during 2017. Physicochemical parameters were measured in situ such as temperature and pH. Likewise, cyanobacteria and microalgae were identified and accounted. The trophic state index (IETP) cataloged the lake as eutrophic and hypertrophic (IETP = 63.80-88.18). 34 genera of phytoplankton distributed in 30 families, 17 orders and 10 classes are reported. Biological indicators, such as Microcystis algal blooms (38.41%), low diatom diversity (Nitzschia, Aulacoseira and Cyclotella), presence of Nitzschia and Scenedesmus microalgae resistant to eutrophication processes, and high concentration of fecal coliforms, up to 24,000 NMP/100 ml, evidenced the low quality of water that occurs in lake Amatitlán. In the dry season, more microalgae diversity was found due to the higher incidence of solar radiation, little water change and the accumulation of organic matter. Even though this varies with changes in the concentration of total nitrogen (NT) and total phosphorus (PT), which enhance the proliferation of toxic cyanobacteria. The lake's biodiversity was low due to its hypereutrophic state. We recommend to put this wastewater treatment plants into operation to prevent this problem to continue.

Phytoplankton/growth & development , Water Quality , Lakes/analysis , Phosphorus/analysis , Biodiversity , Microcystis/growth & development , Coliforms , Eutrophication , Microalgae , Wastewater/toxicity , Nitrogen/analysis
Rev. bras. parasitol. vet ; 29(2): e002220, 2020. tab
Article in English | LILACS | ID: biblio-1138085


Abstract This study aimed to evaluate the effects of Trichostrongylus colubriformis infection on the hemato-biochemical parameters, feed digestibility, and nitrogen balance in Santa Inês lambs. Eighteen three-month-old Santa Ines castrated male lambs (16.9 ± 1.43 kg of body weight) were randomly distributed in two experimental treatments: infected with T. colubriformis (I, n = 9) and uninfected (U, n = 9). The I group received a total of 45,000 L3 larvae of T. colubriformis (5,000 infective larvae, three times per week, for three weeks). During the experimental period, blood, feed digestibility, and nitrogen balance were evaluated. The I lambs showed a reduction in erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, and total proteins, as well as an increase in platelets and eosinophils compared to those in the U group (p < 0.05). With the exception of total protein content, these values were within the normal range for the species. Furthermore, lower dry matter and organic matter digestibility were observed in the I lambs (p = 0.08). The present findings highlight that T. colubriformis infection has the potential to impair some hemato-biochemical parameters as well as feed digestibility in lambs, which could affect their productivity.

Resumo O objetivo deste estudo foi avaliar os efeitos da infecção por Trichostrongylus colubriformis nos parâmetros hemato-bioquímicos, digestibilidade aparente dos nutrientes e balanço de nitrogênio de cordeiros Santa Inês. Dezoito cordeiros Santa Inês, de três meses de idade e castrados (16,9 ± 1,43 kg de peso corporal), foram distribuídos aleatoriamente em dois tratamentos experimentais: infectados com T. colubriformis (I, n = 9) e não infectados (U, n = 9). O grupo I recebeu um total de 45.000 larvas L3 de T. colubriformis (5.000 larvas infectantes, três vezes por semana, durante três semanas). Durante o período experimental, foram avaliadas as variáveis sanguíneas, digestibilidade e balanço de nitrogênio. Os cordeiros I apresentaram redução de eritrócitos, hemoglobina, hematócrito, volume corpuscular médio e proteínas totais, além de aumento de plaquetas e eosinófilos, quando comparados ao grupo U (p< 0,05). Contudo, exceto para proteínas totais, os valores estavam dentro do intervalo normal para a espécie. Além disso, a digestibilidade da matéria seca e da matéria orgânica foi menor no grupo I de cordeiros (p = 0,08). Os presentes achados destacam que a infecção por T. colubriformis teve potencial para prejudicar alguns parâmetros hemato-bioquímicos, bem como a digestibilidade aparente da dieta, o que poderia afetar a produtividade dos cordeiros.

Animals , Male , Trichostrongylosis/parasitology , Trichostrongylus/parasitology , Sheep/parasitology , Blood Proteins/analysis , Digestive System/parasitology , Nitrogen/analysis , Sheep Diseases/parasitology , Trichostrongylosis/urine , Trichostrongylosis/blood , Trichostrongylosis/veterinary , Blood Cell Count/veterinary , Hemoglobins/analysis , Serum Albumin, Bovine/analysis , Serum Globulins/analysis , 3-Hydroxybutyric Acid/blood , Disease Models, Animal , Erythrocyte Indices/veterinary , Feces/parasitology , Feces/chemistry , Nitrogen/urine
Braz. arch. biol. technol ; 63(spe): e20190536, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142500


Abstract Swine manure is applied in agricultural fields as a source of nutrients for plant growth, however, excessive application over the years can promote soil phosphorus (P) accumulation. The objective of this study was to establish the environmental soil P threshold based on the degree of P saturation (DPS), as well, to evaluate the soil P storage capacity. The experiment was carried out in an Oxisol (sandy clay loam texture), under no-tillage and crop rotation. Treatments consisted of four annual doses of liquid swine manure (0, 100, 200, and 300 m3 ha-1 year-1), and three doses of mineral fertilizer (0, 50, and 100% of the crop nutrients requirement), in a randomized block with split-plot design (four replications). Soil P content was analyzed by PMehlich-1, PCaCl2, water-soluble P (WSP) and total P. The application of swine manure and mineral fertilizer increased soil P contents mainly at 0-10 cm depth. The DPS corresponding to the change point was 14.9% at depth 0-10 and 8.6% at depth 0-20 cm with WSP and 18.7% at 0-10 cm and 8.9% at 0-20 cm depth with PCaCl2. The lowest change point value was DPS 8.6% which corresponds to 43 mg kg-1 of PMehlich-1, so, in practical terms, we suggest this value as the environmental soil P threshold. The soil P storage capacity indicated negative values with the higher doses of swine manure and mineral fertilizer which increases the vulnerability of P loss by surface and subsurface hydrological transfer pathway.

Animals , Phosphorus/analysis , Soil/chemistry , Fertilizers/analysis , Manure/analysis , Potassium/analysis , Swine , Models, Theoretical , Nitrogen/analysis
Braz. arch. biol. technol ; 63(spe): e20190063, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142506


Abstract System fertilization is characterized by partial or total fertilizer application at the predecessor crop; and it can be a viable practice to soybean crop. This study aimed to determine the fertilizer management and fertilizer levels for black oat-soybean cropping system, in high fertility soils and no-tillage system. The field trial was conducted in a bifactorial scheme, consisting of six environments, by combination of locations (Bom Sucesso do Sul - Paraná, Itapejara d'Oeste - Paraná) and fertilization management (all fertilization in black oat; splitting with 50% in black oat and 50% in soybean, all fertilization in soybean), and four fertilizer levels (0, 100, 200 and 300%) defined according to soil analysis and production expected. The evaluated traits were dry mass production, N, P and K nutrient accumulation of straw, dry mass remaining of black oat crop; and plant height, number of pods per plant, thousand grain weight, grain yield for soybean crop. Higher black oat dry mass production was observed at higher fertilization level. The fertilizer anticipation in black oat crop had better performance. Phosphorus and potassium accumulation increased linearly with fertilizer level increase. For N, the highest accumulated value occurred at the 200%, decreasing at the 300% of fertilizer level. The soybean crop had no influence in grain yield considering fertilization management, anticipation or splitting, and fertilizer levels. Thus, the system fertilization can be a viable practice, and favor black oat dry mass production and soybean development.

Humans , Glycine max/growth & development , Soil Analysis , Avena/growth & development , Fertilizers , Phosphorus/analysis , Potassium/analysis , Crop Production/methods , Nitrogen/analysis
Braz. arch. biol. technol ; 63(spe): e20190537, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142508


Abstract The main destination of manure is the application to croplands near livestock farms as nutrient source to enhance crop production. The aim of this study was to define the dairy liquid manure (DLM) dose, complementary to the mineral fertilizer, for higher crops yield, in rotation black oat-maize-wheat-soybean, and to identify the soil chemical variables improved by the manure that most affect the yield, in long-term. The experiment was conducted from 2006 to 2015, in no-tillage system, at Paraná State, Brazil. The soil was a Latossolo Bruno Distrófico típico, clayey texture. The treatments consisted by doses of DLM (0, 60, 120 and 180 m3 ha-1 year-1), complementary to the mineral fertilizer (applied in the same amount for all DLM doses). Crops yield and soil chemical variables were evaluated at six depths (0-10; 10-20; 20-30; 30-40; 40-50 and 50-60 cm). The DLM application increased the yield of all crops, but not in all harvests. The DLM even applied at soil surface improved the soil chemical variables in deep layers, resulting in high positive correlation between yield and exchange bases, P, Zn and Mn contents, and high negative correlation with Ca/Mg ratio and potential acidity at depth 0-10 cm. The DLM dose, complementary to the mineral fertilization, that provided higher soybean and wheat yield was about 130 m3 ha-1 year-1, while for maize this dose was equal to or greater than 180 m3 ha-1 year-1. This effect was not attributed to a single chemical variable but the improvement of all chemical variables evaluated.

Humans , Animals , Soil/chemistry , Fertilizers , Crop Production/methods , Manure , Phosphorus/analysis , Glycine max , Triticum , Carbon/analysis , Avena , Crops, Agricultural , Zea mays , Farms , Minerals/analysis , Nitrogen/analysis
Braz. arch. biol. technol ; 63(spe): e20190651, 2020. tab
Article in English | LILACS | ID: biblio-1142512


Abstract To accelerate the recovery of degraded environments, it is necessary to use ecological restoration techniques, which require validation according to the ecosystem conditions where are implemented. This work aimed to evaluate soil microbiological attributes under different ecological restoration technologies in a subtropical forest. The study was conducted at UTFPR-DV, southwest of Paraná, in an ecotone between Mixed Ombrophilous Forest and Semideciduous Seasonal Forest and on an Oxisol. In December 2010, a tillage area of at least 17 years old was isolated and the passive restoration, tree planting and nucleation treatments were installed in 40x54 m plots and four replications. In November 2018 the soil was sampled in these plots and in a native forest area as a reference. There were calculated soil organic carbon content (OCC) and microbiological attributes such as microbial biomass N and C (NMIC and CMIC), basal respiration, fungal spore content and the metabolic (qCO2) and microbial quotient (MICq). It can be concluded that nucleation technology can restore soil microbiological attributes but has not yet reached the conditions of a natural environment. Passive restoration is not a good technology for restoring soil microbiological attributes. The higher contents of CMIC, NMIC, OCC, MICq and fungal spores in the soil under native forest compared to ecological restoration technologies indicate that eight years of adoption of these techniques have not yet been enough to fully recover soil microbiological activity.

Soil Microbiology , Forests , Conservation of Natural Resources/methods , Spores, Fungal , Tropical Climate , Brazil , Carbazoles , Carbon/analysis , Nitrogen/analysis
Braz. arch. biol. technol ; 63(spe): e20190520, 2020. tab, graf
Article in English | LILACS | ID: biblio-1142513


Abstract Conservation agriculture practices can contribute to changes in soil nutrient dynamics over time. This experiment evaluated the changes in total stocks and distribution of carbon, nitrogen, phosphorus and sulfur concentrations in soil, during 60 months, in an integrated crop-livestock system (ICLS) due to anticipated fertilization of sources and doses phosphates applied in soil surface. The experiment was conducted over a period of five years, under Typic Dystrudept, using a randomized block design, in an incomplete factorial scheme (3×3+1), with four replications. Treatments consisted of three sources of P [triple superphosphate (TSP), rock phosphate - Arad (RP) and magnesium thermophosphate (MTP)], along with four doses of P (0, 60, 120 and 180 kg ha-1 P2O5 total). Samples of soil were collected in 0-5, 5-10, 10-15, 15-20 and 20-30 cm layers at 24, 36, 48 and 60 months after beggining of experiment where the following chemical attributes were evaluated: (i) total organic carbon (TOC); (ii) total nitrogen Kjeldahl (TNK); (iii) available P by ion exchange resin method (P-IER); and (iv) available S-SO4 2-. The ICLS conditions provided increased total stocks and concentrations of TOC, TNK, P-IER and S-SO4 2- over time. The applications of different phosphates had no influence on soil TOC concentrations during the five years of experimentation. The concentrations of TNK, P-IER and S-SO4 2- showed an increase in different layers of soil, with the application of sources and doses of P. The P fertilization practice that was anticipated can consist of an efficient management of soil fertility, using properly managed conservation systems.

Humans , Animals , Phosphates/administration & dosage , Soil/chemistry , Crop Production , Soil Analysis , Fertilizers , Animal Husbandry , Phosphorus/analysis , Sulfur/analysis , Carbon/analysis , Nitrogen/analysis
Cienc. tecnol. salud ; 7(1): 129-136, 2020. ^c27 cmilus
Article in Spanish | LILACS | ID: biblio-1121037


El impacto antrópico cercano al lago de Amatitlán ha generado niveles altos de eutrofización que conllevan cambios en la dinámica del ecosistema. Uno de ellos es la proliferación de cianobacterias del género Microcystis que pueden llegar a ser perjudiciales para la fauna y flora de lugar e incluso para los seres humanos. Se presenta el caso de cultivo de un consorcio de fitoplancton, tomado directamente del lago y llevado al laboratorio en condiciones controladas, para medir su consumo y aporte de nitrógeno y fósforo, además de los cambios en los factores fisicoquímicos y la biomasa. Se observó la presencia de diatomeas del genero Nitszchia y cianobacterias como Dolichospermum, con una marcada dominancia de Microcystis sp. Se analizó el porcentaje de cambio en la concentración de nutrientes. Los resultados indican que hubo un aumento en las concentraciones de amonio, nitrato y nitrógeno inorgánico disuelto, mientras que disminuyó el nitrógeno total, el fósforo total y los ortofosfatos. Esto indica que hay aporte de nitrógeno inorgánico, consumo de fósforo y nitrógeno orgánico. El fósforo parece ser el nutriente limitante, ya que, al consumirse en un 90 % la biomasa empieza a decrecer.

The anthropic impact near lake Amatitlán has generated high levels of eutrophication that lead to changes in ecosystem dynamics. One of them is the proliferation of cyanobacteria of the genus Microcystis that can be harmful to the fauna and flora of the place and even to humans. The case of cultivation of a phytoplankton consortium, taken directly from the lake and taken to the laboratory under controlled conditions, to measure its consumption and contribution of nitrogen and phosphorus, in addition to changes in physicochemical factors and biomass is presented. The presence of diatoms of the genus Nitszchia and cyanobacteria such as Dolichospermum was detected, with a marked dominance of Microcystis sp. The percentage change in nutrient concentration was analyzed. The results indicated that there was an increase in the amounts of dissolved inorganic ammonium, nitrate and nitrogen, while total nitrogen, total phosphorus and orthophosphates decreased. This indicates that there is contribution of inorganic nitrogen, consumption of phosphorus and organic nitrogen. Phosphorus seems to be the limiting nutrient, since, when consumed by 90 %, biomass begins to decrease.

Humans , Lakes/analysis , Cyanobacteria , Microcystis , Phosphorus/analysis , Phytoplankton , Eutrophication , Nitrogen/analysis
Braz. j. biol ; 79(4): 589-593, Nov. 2019. tab
Article in English | LILACS | ID: biblio-1001490


Abstract The objective of this study was to evaluate the ability to degrade organic matter by edaphic macrofauna (worms), carbon/nitrogen (C/N) ratio and hydrogenation potential (pH) during the vermicomposting process in different organic residues. The treatments were constituted by organic residues of animal origin (bovine, ovine and equine manure) and vegetable (herb-checkmate and coffee drag), which were conditioned in plastic pots with a capacity of 10 liters, comprising five treatments in a completely randomized experimental design, with five replications. Were inoculated 150 earthworms of the species Eisenia foetida, into each plot. After 87 days, the evaluation of the multiplication of the earthworms was carried out, through its manual count and its cocoons. At the beginning and at the end of the experiment, the samples were submitted to analysis of humidity at 60 °C, pH, volumetric density, chemical analysis of macronutrients and C/N ratio. There was a dominance of worms and cocoons in the process of vermicomposting in the residues of ovine manure and herb-checkmate. The macronutrients (P, K and Mg) and C/N ratio were higher in the vegetal residues, while for N higher values were found in ovine manure and coffee drag treatments, and for Ca higher value among treatments was observed in the coffee drag treatment at the end and the lowest value at initiation. The results obtained in this study demonstrate the importance of the edaphic macrofauna to the vermicomposting process, since it allows more information about its influence on the continuity of soil organic matter decomposition processes.

Resumo Este estudo objetivou avaliar a capacidade de degradar a matéria orgânica pela macrofauna edáfica (minhocas), a relação carbono/nitrogênio (C/N) e o potencial hidrogeniônico (pH), durante o processo da vermicompostagem em diferentes resíduos orgânicos. Os tratamentos foram constituídos por resíduos orgânicos de origem animal (esterco bovino, ovino e equino) e vegetal (resíduo de erva-mate e borra-de-café), os quais foram acondicionados em vasos plásticos com capacidade de 10 litros, compondo cinco tratamentos em delineamento experimental inteiramente casualisado, com cinco repetições. Foram inoculadas 150 minhocas da espécie Eisenia foetida, em cada recipiente. Após 87 dias, foi realizada a avaliação da multiplicação das minhocas, através da sua contagem manual e seus casulos. Os resíduos foram submetidos, ao inicio e ao final do experimento, a análises de umidade a 60 °C, pH, densidade volumétrica, análise química de macronutrientes e relação C/N. Houve uma dominância de minhocas e casulos no processo da vermicompostagem nos resíduos de esterco ovino e erva-mate. Observou-se para os macronutrientes (P, K e Mg) e para a relação C/N uma maior quantidade nos resíduos vegetais, enquanto que para N valores maiores foram encontrados nos tratamentos esterco ovino e borra-de-café, e para Ca o maior valor entre os tratamentos foi observado no tratamento com borra de café ao final e o menor valor no inicio. Os resultados obtidos neste estudo demonstram a importância da macrofauna edáfica para o processo da vermicompostagem por possibilitar maiores informações sobre sua influência na continuidade dos processos de decomposição da matéria orgânica.

Animals , Oligochaeta/physiology , Soil/chemistry , Composting , Garbage , Manure/analysis , Biodegradation, Environmental , Brazil , Cattle , Carbon/analysis , Sheep, Domestic , Horses , Hydrogen-Ion Concentration , Nitrogen/analysis
Braz. j. biol ; 79(1): 15-21, Jan.-Mar 2019. tab, graf
Article in English | LILACS | ID: biblio-984006


Abstract Resource amendments commonly promote plant invasions, raising concerns over the potential consequences of nitrogen (N) deposition; however, it is unclear whether invaders will benefit from N deposition more than natives. Growth is among the most fundamental inherent traits of plants and thus good invaders may have superior growth advantages in response to resource amendments. We compared the growth and allocation between invasive and native plants in different N regimes including controls (ambient N concentrations). We found that invasive plants always grew much larger than native plants in varying N conditions, regardless of growth- or phylogeny-based analyses, and that the former allocated more biomass to shoots than the latter. Although N addition enhanced the growth of invasive plants, this enhancement did not increase with increasing N addition. Across invasive and native species, changes in shoot biomass allocation were positively correlated with changes in whole-plant biomass; and the slope of this relationship was greater in invasive plants than native plants. These findings suggest that enhanced shoot investment makes invasive plants retain a growth advantage in high N conditions relative to natives, and also highlight that future N deposition may increase the risks of plant invasions.

Resumo As alterações de recursos geralmente promovem invasões de plantas, suscitando preocupações quanto às conseqüências potenciais da deposição de nitrogênio (N); No entanto, não está claro se os invasores se beneficiarão da deposição de N mais do que com os nativos. O crescimento é um dos traços inerentes mais fundamentais das plantas e, portanto, os bons invasores podem ter vantagens de crescimento superiores em resposta a alterações de recursos. Comparamos o crescimento e a alocação entre plantas invasivas e nativas em diferentes regimes de N, incluindo controles (concentrações ambientais de N). Descobrimos que as plantas invasivas sempre cresceram muito mais do que as plantas nativas em diferentes condições de N, independentemente das análises baseadas em crescimento ou filogenia, e que o primeiro atribuiu mais biomassa aos rebentos do que o segundo. Embora N aumentou o crescimento de plantas invasivas, esse aumento não aumentou com o aumento da adição de N. Através das espécies invasivas e nativas, as mudanças na alocação da biomassa do extrato foram correlacionadas positivamente com as mudanças na biomassa da planta inteira; e a inclinação desse relacionamento foi maior em plantas invasivas do que plantas nativas. Essas descobertas sugerem que o aumento do investimento em lançamentos faz com que as plantas invasivas mantenham uma vantagem de crescimento em altas condições de N em relação aos nativos, e também destacar que a futura deposição de N pode aumentar os riscos de invasões de plantas.

Soil/chemistry , Magnoliopsida/growth & development , Introduced Species , Nitrogen/analysis , China , Plant Shoots/growth & development , Fertilizers/analysis
Braz. arch. biol. technol ; 62: e19180365, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055401


Abstract In this study, we aim to determine the shelf life of dried and ready to use-powdered soup samples obtained from different types of fish species such as Rainbow trout (Oncorhynchus mykiss), sea bream (Sparusaurata L.) and sea bass (Dicentrarchuc labrax). For this purpose, the chemical (moisture, protein, fat, carbohydrate, ash, pH, Thiobarbituric acid (TBA), Total volatile basic nitrogen (TVB-N), microbiological (total mesophilic aerobic bacteria, total yeast and mold, total coliform, Staphylococcus spp. and Salmonella spp.) and sensory properties of fish soup samples were determined during 6-month storage period. The pH, TBA and TVB-N values of all samples increased during the storage period, but these values were within the consumption limits. Additionally, the microbiological properties of all fish soup samples were found within the consumption limits during storage. As a result, a product has been obtained with high nutritional value and rich with regard to protein, oil, and minerals by adding the fish meat to soup samples. It has been determined that the product quality and shelf life significantly increased by using the boiled product for the production of soup samples.

Thiobarbiturates/analysis , Soups , Food Storage/standards , Fishes , Nitrogen/analysis
Braz. j. microbiol ; 49(4): 832-839, Oct.-Dec. 2018. tab, graf
Article in English | LILACS | ID: biblio-974313


ABSTRACT Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0-3.20 g L-1), threonine (0.0-1.44 g L-1), ornithine (0.0-4.08 g L-1), and glutamate (0.0-8.16 g L-1), according to a two-level central composite rotatable design. A medium containing ferrous sulfate yielded CA production of 437 mg L-1, while a formulation without this salt produced only 41 mg L-1 of CA. This substantial difference suggested that Fe2+ is important for CA biosynthesis. The experimental design showed that glutamate and ornithine negatively influenced CA production while arginine and threonine had no influence. The soybean protein isolate provided sufficient C5 precursor for CA biosynthesis, so that supplementation was unnecessary. Screening of medium components, together with experimental design tools, could be a valuable way of enhancing CA titers and reducing the process costs.

Streptomyces/metabolism , Clavulanic Acid/biosynthesis , Culture Media/metabolism , Ornithine/analysis , Ornithine/metabolism , Streptomyces/genetics , Glutamic Acid/analysis , Glutamic Acid/metabolism , Culture Media/chemistry , Nitrogen/analysis , Nitrogen/metabolism
Braz. j. microbiol ; 49(3): 443-451, July-Sept. 2018. tab, graf
Article in English | LILACS | ID: biblio-951793


Abstract As a glacier retreats, barren areas are exposed, and these barren areas are ideal sites to study microbial succession. In this study, we characterized the soil culturable bacterial communities and biochemical parameters of early successional soils from a receding glacier in the Tianshan Mountains. The total number of culturable bacteria ranged from 2.19 × 105 to 1.30 × 106 CFU g-1 dw and from 9.33 × 105 to 2.53 × 106 CFU g-1 dw at 4 °C and 25 °C, respectively. The number of culturable bacteria in the soil increased at 25 °C but decreased at 4 °C along the chronosequence. The total organic carbon, total nitrogen content, and enzymatic activity were relatively low in the glacier foreland. The number of culturable bacteria isolated at 25 °C was significantly positively correlated with the TOC and TN as well as the soil urease, protease, polyphenoloxidase, sucrase, catalase, and dehydrogenase activities. We obtained 358 isolates from the glacier foreland soils that clustered into 35 groups using amplified ribosomal DNA restriction analysis. These groups are affiliated with 20 genera that belong to six taxa, namely, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroides, and Deinococcus-Thermus, with a predominance of members of Actinobacteria and Proteobacteria in all of the samples. A redundancy analysis showed that the bacterial succession was divided into three periods, an early stage (10a), a middle stage (25-74a), and a late stage (100-130a), with the total number of culturable bacteria mainly being affected by the soil enzymatic activity, suggesting that the microbial succession correlated with the soil age along the foreland.

Bacteria/isolation & purification , Ice Cover/microbiology , Ice Cover/chemistry , Phylogeny , Soil/chemistry , Soil Microbiology , Bacteria/classification , Bacteria/growth & development , Bacteria/genetics , DNA, Bacterial/genetics , DNA, Ribosomal/genetics , RNA, Ribosomal, 16S/genetics , China , Sequence Analysis, DNA , Nitrogen/analysis , Nitrogen/metabolism