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1.
Article in English | WPRIM | ID: wpr-762186

ABSTRACT

MicroRNAs (miRs) are single-stranded RNAs of 18-25 nucleotides. These molecules regulate gene expression at the post-transcriptional level; several of these are differentially expressed in asthma as well as in viral acute respiratory infections (ARIs), the main triggers of acute asthma exacerbations. In recent years, miRs have been studied in order to discover drug targets as well as biomarkers for diagnosis, disease severity and prognosis. We describe recent findings on miR expression and function in asthma and their role in the regulation of viral ARIs, according to cell tissue specificity and asthma severity. By combining the above information, we identify miRs that may be important in virus-induced asthma exacerbations. This is the first attempt to link miR profiles of asthmatic patients and ARI-induced miRs, addressing the question of whether there might be a specific miR deficit in asthmatic subjects that make them more susceptible and/or reactive to infection.


Subject(s)
Asthma , Biomarkers , Diagnosis , Disease Progression , Gene Expression , Humans , Inflammation , MicroRNAs , Nucleotides , Organ Specificity , Prognosis , Respiratory Tract Infections , RNA
2.
Article in English | WPRIM | ID: wpr-776623

ABSTRACT

Although the foundations and evolution of Chinese medicine and Western medicine are very different, an increasing amount of research has revealed that those Eastern medicine principles practiced over thousands of years are confirmed by new technologies applied to the basic science of the human body. Recent scientific discoveries present enticing opportunities to reconcile Chinese medicine theories with Western biomedicine. Is there a trend toward the convergence of Eastern and Western medicine? Four studies which exemplify the potential for convergence are described in this article. The studies present findings in regard to mesentery, interstitium, a gut-lung axis, and lung-centered hematopoiesis, and were published recently in leading journals such as Science, Nature, and Lancet.


Subject(s)
Hematopoiesis , Humans , Medicine, Chinese Traditional , Meridians , Organ Specificity
3.
Journal of Breast Disease ; (2): 30-37, 2019.
Article in English | WPRIM | ID: wpr-764286

ABSTRACT

PURPOSE: We aimed to investigate organ-specific recurrence or the metastatic pattern of breast cancer according to biological subtypes and clinical characteristics. METHODS: We retrospectively analyzed the medical records of 168 patients with recurrent breast cancer who were diagnosed between January 1, 2000 and April 30, 2017. Four biological subtypes were classified according to estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER2), and Ki-67 expression: luminal A, luminal B, HER2-enriched, and triple negative breast cancer (TNBC). To analyze recurrence patterns according to biological subtypes, we accessed clinical variables including age at diagnosis, TNM stage, type of surgery in the breast and axilla, histologic grade, nuclear grade, lymphatic, vascular, and neural invasion, Ki-67 expression and recurrence to distant organs. RESULTS: The biological subtypes of recurrent breast cancer comprised the following luminal A (n=33, 19.6%), luminal B (n=95, 56.5%), HER2 enriched (n=19, 11.3%), and TNBC (n=21, 12.5%). Luminal A (7.7%) and B (6.5%) subtypes were associated with the increased rate of local recurrence compared to HER2-enriched (2.4%) and TNBC subtypes (1.8%) (p=0.005). The bone (53.6%) was the most common metastatic organ, followed by the lung (34.5%), liver (29.8%), brain (17.9%), and other visceral organ (7.7%). Bone metastasis was commonly observed in individuals with luminal B (63.2%), HER2-enriched (57.9%), and luminal A (42.4%) subtypes (p=0.005). Most liver metastases occur in individuals with luminal B (40.0%) and HER2-enriched subtypes (31.6%) (p=0.002). CONCLUSION: Luminal B subtype was commonly observed in individuals with recurrent breast cancer, and the bone is the most common target organ for breast cancer metastasis, followed by the lungs and liver.


Subject(s)
Axilla , Brain , Breast Neoplasms , Breast , Diagnosis , Estrogens , Humans , Liver , Lung , Medical Records , Neoplasm Metastasis , Organ Specificity , Phenobarbital , ErbB Receptors , Receptors, Progesterone , Recurrence , Retrospective Studies , Triple Negative Breast Neoplasms
4.
Article in English | WPRIM | ID: wpr-772996

ABSTRACT

The mA modification has been implicated as an important epitranscriptomic marker, which plays extensive roles in the regulation of transcript stability, splicing, translation, and localization. Nevertheless, only some genes are repeatedly modified across various conditions and the principle of mA regulation remains elusive. In this study, we performed a systems-level analysis of human genes frequently regulated by mA modification (mAfreq genes) and those occasionally regulated by mA modification (mAocca genes). Compared to the mAocca genes, the mAfreq genes exhibit gene importance-related features, such as lower dN/dS ratio, higher protein-protein interaction network degree, and reduced tissue expression specificity. Signaling network analysis indicates that the mAfreq genes are associated with downstream components of signaling cascades, high-linked signaling adaptors, and specific network motifs like incoherent feed forward loops. Moreover, functional enrichment analysis indicates significant overlaps between the mAfreq genes and genes involved in various layers of gene expression, such as being the microRNA targets and the regulators of RNA processing. Therefore, our findings suggest the potential interplay between mA epitranscriptomic regulation and other gene expression regulatory machineries.


Subject(s)
Adenosine , Metabolism , Gene Expression Regulation , Gene Regulatory Networks , Humans , MicroRNAs , Metabolism , Organ Specificity , Signal Transduction
5.
Article in English | WPRIM | ID: wpr-772990

ABSTRACT

Sex differences are widely observed under various circumstances ranging from physiological processes to therapeutic responses, and a myriad of sex-biased genes have been identified. In recent years, transcriptomic datasets of microRNAs (miRNAs), an important class of non-coding RNAs, become increasingly accessible. However, comprehensive analysis of sex difference in miRNA expression has not been performed. Here, we identified the differentially-expressed miRNAs between males and females by examining the transcriptomic datasets available in public databases and conducted a systemic analysis of their biological characteristics. Consequently, we identified 73 female-biased miRNAs (FmiRs) and 163 male-biased miRNAs (MmiRs) across four tissues including brain, colorectal mucosa, peripheral blood, and cord blood. Our results suggest that compared to FmiRs, MmiRs tend to be clustered in the human genome and exhibit higher evolutionary rate, higher expression tissue specificity, and lower disease spectrum width. In addition, functional enrichment analysis of miRNAs show that FmiR genes are significantly associated with metabolism process and cell cycle process, whereas MmiR genes tend to be enriched for functions like histone modification and circadian rhythm. In all, the identification and analysis of sex-biased miRNAs together could provide new insights into the biological differences between females and males and facilitate the exploration of sex-biased disease susceptibility and therapy.


Subject(s)
Biological Evolution , Female , Genome, Human , Humans , Male , MicroRNAs , Blood , Genetics , Organ Specificity , Sex Characteristics , Transcriptome
6.
Immune Network ; : e5-2018.
Article in English | WPRIM | ID: wpr-740204

ABSTRACT

Chemokine (C-X3-C motif) ligand 1 (CX₃CL1, also known as fractalkine) and its receptor chemokine (C-X3-C motif) receptor 1 (CX₃CR1) are widely expressed in immune cells and non-immune cells throughout organisms. However, their expression is mostly cell type-specific in each tissue. CX₃CR1 expression can be found in monocytes, macrophages, dendritic cells, T cells, and natural killer (NK) cells. Interaction between CX3CL1 and CX₃CL1 can mediate chemotaxis of immune cells according to concentration gradient of ligands. CX₃CL1 expressing immune cells have a main role in either pro-inflammatory or anti-inflammatory response depending on environmental condition. In a given tissue such as bone marrow, brain, lung, liver, gut, and cancer, CX₃CL1 expressing cells can maintain tissue homeostasis. Under pathologic conditions, however, CX₃CL1 expressing cells can play a critical role in disease pathogenesis. Here, we discuss recent progresses of CX3CL1/CX₃CL1 in major tissues and their relationships with human diseases.


Subject(s)
Bone Marrow , Brain , Chemokine CX3CL1 , Chemotaxis , Dendritic Cells , Homeostasis , Humans , Ligands , Liver , Lung , Macrophages , Monocytes , Organ Specificity , T-Lymphocytes
7.
Rev. gaúch. enferm ; 38(4): e68716, 2017. tab, graf
Article in Portuguese | LILACS, BDENF | ID: biblio-960780

ABSTRACT

Resumo OBJETIVO Mapear a produção de conhecimento acerca das complicações do acesso vascular em pacientes submetidos a procedimentos percutâneos em Laboratório de Hemodinâmica. MÉTODOS Estudo do tipo revisão de escopo. Elaborou-se estratégia de busca em três etapas, considerando o período entre julho de 2005 e 2015, nas bases de dados PubMed, CINAHL, Scopus e LILACS. Os dados extraídos foram analisados e sintetizados de forma narrativa. RESULTADOS Foram incluídas 128 publicações que permitiram mapear os contextos de estudo das complicações, a ocorrência de acordo com as vias, bem como a compreensão do diagnóstico e manejo clínico. Como síntese da análise identificou-se três categorias temáticas: Complicações; Fatores preditores; e Diagnóstico/tratamento. CONCLUSÃO As complicações no local do acesso vascular são de ocorrência variável conforme a via de acesso utilizada. O conhecimento dos fatores que permeiam a ocorrência destes eventos podem auxiliar no reconhecimento precoce, planejamento e monitorização dos cuidados implementados.


Resumen OBJETIVO Mapear la producción de conocimiento acerca de las complicaciones del acceso vascular en pacientes sometidos a procedimientos percutâneos en el Laboratorio de Hemodinamia. MÉTODOS Estudio de tipo revisión de escopo. Se elaboró la estrategia de búsqueda en tres etapas, considerando el período comprendido entre julio 2005 y 2015, en las bases PubMed, CINAHL, Scopus y LILACS. Los datos extraídos fueron analizados y sintetizados de forma narrativa. RESULTADOS Fueron incluidas 128 publicaciones que permitieron mapear los contextos de estudio de las complicaciones, la ocurrencia de acuerdo con las vías, así como la comprensión del diagnóstico y manejo clínico. Como síntesis del análisis se identificó tres categorías temáticas: Complicaciones, Factores predictores y Diagnóstico/tratamiento. CONCLUSIÓN Las complicaciones en el sitio del acceso vascular son de ocurrencia variable de acuerdo con la vía de acceso utilizada. El conocimiento de los factores que están presentes en la ocurrencia de estos eventos puede auxiliar en el reconocimiento temprano, planeamiento y control de la atención implementados.


Abstract OBJECTIVE To map the production of knowledge on vascular access complications in patients undergoing percutaneous procedures in hemodynamic laboratories. METHODS Scoping review study. The search strategy was developed in three stages, considering the period from July 2005 to July 2015 in the PubMed, CINAHL, Scopus, and LILACS databases. The collected data were analyzed and summarized in a narrative form. RESULTS One-hundred twenty-eight publications that made it possible to map the contexts of study of complications, occurrence according to access routes, as well as an understanding of diagnosis and clinical management, were included. Three theme categories were identified: complications; predictive factors; and diagnosis/treatment. CONCLUSION Vascular access site complications range according to the access route used. Knowledge of factors that permeate the occurrence of these events may contribute to early detection, planning, and monitoring of the care implemented.


Subject(s)
Humans , Vascular Access Devices/adverse effects , Hemodynamics , Organ Specificity , Wound Infection , Punctures/adverse effects , Risk Factors , Aneurysm, False/etiology , Hemorrhage/etiology
8.
Acta cir. bras ; 31(4): 278-285, Apr. 2016. tab, graf
Article in English | LILACS | ID: lil-781333

ABSTRACT

PURPOSE: To investigate gender differences in the evolution of the inflammatory process in rats subjected to brain death (BD). METHODS: Adult Wistar rats were divided into three groups: female; ovariectomized female; and male rats. BD was induced using intracranial balloon inflation and confirmed by maximal pupil dilatation, apnea, absence of reflex, and drop of mean arterial pressure. Six hours after BD, histological evaluation was performed in lungs, heart, liver and kidneys, and levels of inflammatory proteins, estrogen, progesterone, and corticosterone were determined in plasma. RESULTS: In the lungs, females presented more leukocyte infiltration compared to males (p<0.01). Ovariectomized female rat lungs were more hemorrhagic compared to other groups (p<0.001). In the heart, females had higher leukocyte infiltration and tissue edema compared to males (p<0.05). In the liver and kidneys, there were no differences among groups. In female group estradiol and progesterone were sharply reduced 6 hours after BD (p<0.001) to values observed in ovariectomized females and males. Corticosterone levels were similar. CONCLUSIONS: Sex hormones influence the development of inflammation and the status of organs. The increased inflammation in lungs and heart of female rats might be associated with the acute reduction in female hormones triggered by BD.


Subject(s)
Animals , Male , Female , Brain Death/pathology , Sex Characteristics , Kidney/pathology , Liver/pathology , Lung/pathology , Myocardium/pathology , Organ Specificity , Progesterone/blood , Reference Values , Time Factors , Ovariectomy , Sex Factors , Rats, Wistar , Edema/pathology , Estradiol/blood , Chemokine CXCL1/analysis , Chemokine CXCL2/analysis , Inflammation/pathology
9.
Article in Chinese | WPRIM | ID: wpr-263993

ABSTRACT

<p><b>OBJECTIVE</b>To examine the expression patterns of short palate, lung and nasal epithelium clone 1 (SPLUNC1) gene in human tissues.</p><p><b>METHODS</b>In situ hybridization was used to detect the expression of SPLUNC1 gene in 37 different human tissues.</p><p><b>RESULTS</b>We found that SPLUNC1 gene was not expressed in squamous epithelial cells of the palate, epidermis, esophagus, or the esophagus-cardia junction, metaplastic squamous cells in the nasopharynx, trachea, or uterus cervix, or tumor cells of esophageal squamous cell carcinoma or lung squamous cell carcinoma. SPLUNC1 gene was not expressed in the single layer columnar epithelia cells in the stomach, gallbladder, jejunum, colon, endometrium, or uterus cervix. SPLUNC1 expression was detected mainly in pseudostratified columnar epithelial cells in the nasopharynx, trachea and bronchi, and was gradually down-regulated from the upper to lower end of the respiratory tract, but was not detected in the lung tissues. SPLUNC1 expression was detected not only in the duct and serous gland cells in the parotid and submandibular glands, but also in cells of submucosal serous glands in the nasopharynx and lung, but not in the cells of the mucosal glands. The parietal cells of the gastric submucosa and epithelial cells of the lobula and ducts of the mammary glands expressed SPLUNC1. The adenocarcinoma cells in the lung, stomach, colon, mammary gland, uterus endometrium and cervix showed strong expressions of SPLUNC1 gene.</p><p><b>CONCLUSION</b>SPLUNC1 expression is highly cell-specific in association with the cell functions.</p>


Subject(s)
Epithelial Cells , Metabolism , Gene Expression , Glycoproteins , Genetics , Metabolism , Humans , Organ Specificity , Phosphoproteins , Genetics , Metabolism
10.
Article in English | WPRIM | ID: wpr-287176

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the protective effects and mechanisms of Radix Astragali Injection on multiple organs of rats with obstructive jaundice (OJ).</p><p><b>METHODS</b>A total of 180 rats were randomly divided into the sham-operated, model control and treated groups (60 in each group). On 7, 14, 21 and 28 days after operation, the serum contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), r-glutamyl transpeptidase (r-GT), total bilirubin (TBil), direct bilirubin (DBil), blood urine nitrogen (BUN), and creatinine (CREA) were determined. And the pathological changes of livers, kidneys and lungs, and protein expressions of toll-like receptor-4 (TLR-4) of livers, intercellular adhesion molecule-1 (ICAM-1) of lungs, Bax and nuclear factor-kappa B (NF-κB), as well as apoptotic indexes of multiple organs were observed, respectively.</p><p><b>RESULTS</b>The pathological severity scores of multiple organs (including livers on 7, 14, 21 and 28 days, kidneys on 14 and 28 days, and lungs on 14 days), serum contents of ALT (14 and 21 days), AST (14 days), TBil (7, 14, 21 and 28 days), DBil (14 and 21 days), BUN (28 days), protein expressions of TLR-4 (in livers, 28 days), Bax (in livers and kidneys, 21 days), and apoptotic indexes in livers (7 and 21 days) in the treated group were significantly lower than those in the model control group (P<0.05 or P<0.01).</p><p><b>CONCLUSION</b>Radix Astragali Injection exerts protective effects on multiple organs of OJ rats by improving the pathological changes of lung, liver and kidney, decreasing the serum index of hepatic and renal function as well as inhibiting the protein expression of TLR-4 and Bax in the livers and Bax in the kidneys.</p>


Subject(s)
Alanine Transaminase , Blood , Animals , Apoptosis , Aspartate Aminotransferases , Blood , Bilirubin , Blood , Blood Urea Nitrogen , Creatinine , Blood , Drugs, Chinese Herbal , Pharmacology , Therapeutic Uses , Immunohistochemistry , Injections , Intercellular Adhesion Molecule-1 , Metabolism , Jaundice, Obstructive , Blood , Drug Therapy , Kidney , Pathology , Liver , Pathology , Lung , Pathology , Male , NF-kappa B , Metabolism , Organ Specificity , Plant Extracts , Pharmacology , Protective Agents , Pharmacology , Therapeutic Uses , Rats, Sprague-Dawley , Toll-Like Receptor 4 , Metabolism , bcl-2-Associated X Protein , Metabolism , gamma-Glutamyltransferase , Metabolism
11.
Article in English | WPRIM | ID: wpr-287104

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the acute and sub-chronic toxicity of intravenously administered tetrandrine (TET) in female BALB/c mice.</p><p><b>METHODS</b>The median lethal dose (LD) of intravenously administered TET was calculated in mice using Dixon's up-and-down method. In the acute toxicity study, mice were intravenously administered with TET at a single dose of 20, 100, 180, 260 and 340 mg/kg, respectively and were evaluated at 14 days after administration. In the sub-acute toxicity study, mice were intravenously administered various doses of TET (30, 90 and 150 mg/kg) each day for 14 consecutive days. Clinical symptoms, mortality, body weight, serum biochemistry, organ weight and histopathology were examined at the end of the experiment, as well as after a 1-week recovery period.</p><p><b>RESULT</b>LDwas found to be 444.67±35.76 mg/kg. In the acute toxicity study, no statistically signifificant differences in body weight, blood biochemistry, or organ histology were observed between the administration and control groups when mice were intravenously administered with single dose at 20, 100, 180, 260 and 340 mg/kg of TET (P >0.05). In the sub-acute toxicity study, no signifificant changes in body weight, biochemistry and organ histology were observed with up to 90 mg/kg of TET compared with the control group (P >0.05), however, in the 150 mg/kg administered group, TET induced transient toxicity to liver, lungs and kidneys, but withdrawal of TET can lead to reversal of the pathological conditions.</p><p><b>CONCLUSIONS</b>The overall fifindings of this study indicate that TET is relatively non-toxic from a single dose of 20, 100, 180, 260 or 340 mg/kg, and that up to 90 mg/kg daily for 14 consecutive days can be considered a safe application dose.</p>


Subject(s)
Administration, Intravenous , Animals , Benzylisoquinolines , Toxicity , Body Weight , Female , Mice, Inbred BALB C , Organ Specificity , Toxicity Tests, Acute , Toxicity Tests, Chronic
12.
National Journal of Andrology ; (12): 175-179, 2016.
Article in Chinese | WPRIM | ID: wpr-304730

ABSTRACT

The G protein-coupled estrogen receptor (GPER), also known as G protein-coupled receptor 30 (GPR30), was identified in the recent years as a functional membrane receptor different from the classical nuclear estrogen receptors. This receptor is widely expressed in the cortex, cerebellum, hippocampus, heart, lung, liver, skeletal muscle, and the urogenital system. It is responsible for the mediation of nongenomic effects associated with estrogen and its derivatives, participating in the physiological activities of the body. The present study reviews the molecular structure, subcellular localization, signaling pathways, distribution, and function of GPER in the male reproductive system.


Subject(s)
Estrogens , Metabolism , Genitalia, Male , Metabolism , Humans , Male , Molecular Structure , Organ Specificity , Receptors, Estrogen , Chemistry , Physiology , Receptors, G-Protein-Coupled , Chemistry , Physiology , Reproduction , Physiology , Signal Transduction
13.
Article in English | WPRIM | ID: wpr-110500

ABSTRACT

Claudins, which are known as transmembrane proteins play an essential role in tight junctions (TJs) to form physical barriers and regulate paracellular transportation. To understand equine diseases, it is helpful to measure the tissue-specific expression of TJs in horses. Major equine diseases such as colic and West Nile cause damage to TJs. In this study, the expression level and distribution of claudin-1, -2, -4, and -5 in eight tissues were assessed by Western blotting and immunohistochemistry methods. Claudin-1 was primarily identified in the lung, duodenum, and uterus, claudin-2 was evenly observed in equine tissues, claudin-4 was abundantly detected in the liver, kidney and uterus, and claudin-5 was strongly expressed in the lung, duodenum, ovary, and uterus, as determined by Western blotting method. The localization of equine claudins was observed by immunohistochemistry methods. These findings provide knowledge regarding the expression patterns and localization of equine claudins, as well as valuable information to understand tight junction-related diseases according to tissue specificity and function of claudins in horses.


Subject(s)
Animals , Architectural Accessibility , Blotting, Western , Claudin-1 , Claudin-2 , Claudin-4 , Claudin-5 , Claudins , Colic , Duodenum , Female , Horse Diseases , Horses , Immunohistochemistry , Kidney , Liver , Lung , Methods , Organ Specificity , Ovary , Tight Junctions , Transportation , Uterus
15.
Einstein (Säo Paulo) ; 13(2): 326-329, Apr-Jun/2015. graf
Article in English | LILACS | ID: lil-751413

ABSTRACT

ABSTRACT The hepatobiliary-specific contrast medium (gadoxetic acid – Primovist®) is primarily used to improve detection and characterization of focal hepatic lesions, such as in chronic liver disease patients with suspected hepatocellular carcinoma. Since the contrast medium is selectively taken up by functioning hepatocytes in the late hepatobiliary phase, it helps to detect typical hepatocellular carcinoma, which show low signal intensity on this phase. This imaging feature also assists in differentiating regenerative/dysplastic nodules from early hepatocellular carcinomas (with over 90% accuracy), as well as hypervascular hepatocellular carcinomas from arterial pseudo-enhancement foci. Future perspectives include its use in quantification of hepatic function and fibrosis.


RESUMO O contraste hepato-específico (ácido gadoxético – Primovist®) tem como utilidade principal melhorar a detecção e a caracterização de lesões hepáticas focais, por exemplo, em hepatopatas crônicos com suspeita de hepatocarcinoma. Por apresentar captação seletiva por hepatócitos funcionantes na fase hepatobiliar tardia, auxilia na detecção de hepatocarcinomas típicos – a maioria dos quais apresentando hipossinal nessa fase. Essa característica de imagem também auxilia na diferenciação entre nódulos regenerativos/ displásicos e hepatocarcinomas precoces (com mais de 90% de acurácia), e entre hepatocarcinomas hipervascularizados e focos de pseudorrealce arterial. Perspectivas futuras promissoras incluem sua utilização na quantificação de função e de fibrose hepáticas.


Subject(s)
Animals , Humans , Contrast Media , Carcinoma, Hepatocellular/diagnosis , Gadolinium DTPA , Liver Neoplasms/diagnosis , Magnetic Resonance Imaging/methods , Chronic Disease , Carcinoma, Hepatocellular/pathology , Contrast Media/pharmacokinetics , Diagnosis, Differential , Gadolinium DTPA/pharmacokinetics , Image Enhancement/methods , Liver Cirrhosis/pathology , Liver Neoplasms/pathology , Organ Specificity , Organic Anion Transporters , Sensitivity and Specificity
16.
Protein & Cell ; (12): 722-734, 2015.
Article in English | WPRIM | ID: wpr-757184

ABSTRACT

Macroautophagy is an evolutionarily conserved intracellular degradation system used by life ranging from yeasts to mammals. The core autophagic machinery is composed of ATG (autophagy-related) protein constituents. One particular member of the ATG protein family, Atg7, has been the focus of recent research. Atg7 acts as an E1-like activating enzyme facilitating both microtubule-associated protein light chain 3 (LC3)-phosphatidylethanolamine and ATG12 conjugation. Thus, Atg7 stands at the hub of these two ubiquitin-like systems involving LC3 and Atg12 in autophagic vesicle expansion. In this review, I focus on the pleiotropic function of Atg7 in development, maintenance of health, and alternations of such control in disease.


Subject(s)
Animals , Disease , Growth and Development , Humans , Organ Specificity , Species Specificity , Ubiquitin-Activating Enzymes , Metabolism
17.
ABCD arq. bras. cir. dig ; 28(1): 53-56, 2015. tab, graf
Article in English | LILACS | ID: lil-742746

ABSTRACT

BACKGROUND: In traditional laparoscopic cholecistectomy, the cystic duct and artery are commonly closed by metallic clips just before their division. Although the placement of these clips for occluding cystic artery and duct can be considered safe, biliary leaks and bleeding may occur especially by its dislodgement. AIM: To report a prospective case-series in total clipless cholecystectomy by means of harmonic shears for closure and division of the artery and cystic duct as well removal of the gallbladder from the liver. METHODS: Was evaluate a series of 125 patients who underwent laparoscopic cholecystectomy where the sealing and division of cystic artery and duct was carried out only by harmonic shears. The intact extracted gallbladder was submitted to a reverse pressure test for assessment of the technique safety by means of CO2 insuflation. RESULTS: The most common indication for surgery was gallstones. The mean operative time was 26 min and all gallbladders were dissected intact from the liver bed. There was no mortality and the overall morbidity rate was 0.8% with no hemorrhage or leaks. The reverse pressure test showed that all specimens support at least 36-mmHg of pressure without leaking. CONCLUSION: The harmonic shears is effective and safe in laparoscopic cholecystectomy as a sole instrument for sealing and division of the artery and cystic duct. The main advantages could be related to the safety and decreased operative time. .


RACIONAL: A colecistectomia laparoscópica na técnica tradicional oclui o ducto cístico e a artéria cística por clipes cirúrgicos, que podem se deslocar ou desprender no pós-operatório, possibilitando a ocorrência de fístula biliar ou hemorragia. OBJETIVO: Relato prospectivo de série de casos de colecistectomias laparoscópicas sem uso de clipe cirúrgico, sendo que a ligadura e secção da artéria cística e do ducto cístico foram realizadas por meio de bisturi ultrassônico. MÉTODO: Foram incluídos 125 pacientes submetidos à colecistectomia laparoscópica sem utilização de clipe cirúrgico metálico, onde a ligadura da artéria e do ducto cístico e também a remoção da vesícula biliar de seu leito hepático foram realizadas por meio de tesoura ultrassônica. Realizou-se teste de pressão reversa na vesícula biliar removida intacta do leito hepático para verificar a segurança da técnica. RESULTADOS: A principal indicação cirúrgica foi a colelitíase. O tempo cirúrgico médio foi de 26 min e todas as vesículas biliares foram retiradas intactas do leito hepático. Não houve mortalidade e a taxa global de morbidade foi de 0,8%, sem hemorragias ou fístulas. O teste de pressão reversa mostrou que o ducto cístico ocluído pelo bisturi harmônico suportou ao pelo menos 36 mmHg de pressão sem que ocorresse nenhum vazamento. CONCLUSÃO: O bisturi harmônico é eficaz e seguro em colecistectomias laparoscópicas eletivas como um instrumento único para ocluir e seccionar tanto a artéria cística quanto o ducto cístico. Vantagens podem ser apontadas ao método com relação a sua segurança e diminuição do tempo cirúrgico. .


Subject(s)
Animals , Humans , Drosophila Proteins/metabolism , Drosophila melanogaster/drug effects , Drosophila melanogaster/physiology , Sodium Chloride/pharmacology , Stress, Physiological/drug effects , Symporters/metabolism , Bacterial Proteins/metabolism , Carbohydrate Metabolism/drug effects , Cell Membrane/drug effects , Cell Membrane/metabolism , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Feeding Behavior/drug effects , Genes, Insect , Gene Expression Regulation/drug effects , Ion Transport/drug effects , Luminescent Proteins/metabolism , Oligonucleotide Array Sequence Analysis , Organ Specificity/drug effects , Phylogeny , Reproducibility of Results , RNA Interference/drug effects , Survival Analysis , Sodium Chloride, Dietary/pharmacology , Time Factors
18.
Article in English | WPRIM | ID: wpr-262646

ABSTRACT

<p><b>OBJECTIVE</b>To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma.</p><p><b>METHODS</b>The inhibitory effect of TAAs was demonstrated in H22-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis.</p><p><b>RESULTS</b>The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 µg/mL (15.13-26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs.</p><p><b>CONCLUSION</b>TAAs is effective for hepatocarcinoma, via inhibiting proliferation and inducing apoptosis.</p>


Subject(s)
Acetogenins , Chemistry , Pharmacology , Therapeutic Uses , Animals , Annona , Chemistry , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Therapeutic Uses , Apoptosis , Carcinoma, Hepatocellular , Drug Therapy , Pathology , Caspases , Metabolism , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Chromatography, High Pressure Liquid , Dose-Response Relationship, Drug , Humans , Liver Neoplasms , Drug Therapy , Pathology , Male , Membrane Potential, Mitochondrial , Mice , Organ Specificity , Spleen , Thymus Gland , Xenograft Model Antitumor Assays
19.
Chinese Journal of Virology ; (6): 379-387, 2015.
Article in Chinese | WPRIM | ID: wpr-296273

ABSTRACT

The severe fever with thrombocytopenia syndrome virus (SFTSV) is the causative pathogen of an emerging infectious disease severe fever with thrombocytopenia syndrome and a new member in the genus Phlebovirus of family Bunyaviridae. Immune responses and pathological lesions in SFTSV-infected Balb/C mice and hamsters were evaluated by inoculation of SFTSV at 105 TCID50 or 103 TCID50 per animal through four different routes of infection, including intravenous, intramuscular, intraperitoneal, and intracerebral injections. The vehicle control groups were also included. At different time points after the inoculation blood and plasma samples were collected. Blood cell counts, blood viral RNA copies, and plasma antibodies were detected by automatic blood cell counters, real-time PCR, and luminex assays, respectively. At two weeks post inoculation, the animals were sacrificed. Tissues including heart, liver, spleen, lung, kidney, intestine, muscle, and brain, were collected for pathological analyses. Results showed that the SFTSV could infect Balb/C mice and hamsters with SFTSV-specific immunoglobulin (Ig) M and IgG antibodies detected in plasma samples on day 7 post inoculation. The SFTSV-specific IgM levels peaked on day 7 post inoculation and then decreased, whereas the SFTSV-specific IgG levels started to increase on day 7 and then peaked on day 14 post inoculation. Pathological analyses indicated significant pathological lesions in liver and kidney tissues. In conclusion, SFTSV could can infect different strains of rodent animals and cause similar immunological and pathological responses.


Subject(s)
Animals , Antibody Specificity , Bunyaviridae Infections , Blood , Pathology , Cricetinae , Immunoglobulin G , Blood , Immunoglobulin M , Blood , Leukocyte Count , Mice , Mice, Inbred BALB C , Organ Specificity , Phlebovirus , Allergy and Immunology , Physiology
20.
Article in English | WPRIM | ID: wpr-146124

ABSTRACT

IgG4-related disease (IgG4-RD) is a potentially multiorgan disorder. In this study, clinical and serological features from 132 IgG4-RD patients were compared about organ correlations. Underlying pathologies comprised autoimmune pancreatitis (AIP) in 85 cases, IgG4-related sclerosing cholangitis (IgG4-SC) in 12, IgG4-related sialadenitis (IgG4-SIA) in 56, IgG4-related dacryoadenitis (IgG4-DAC) in 38, IgG4-related lymphadenopathy (IgG4-LYM) in 20, IgG4-related retroperitoneal fibrosis (IgG4-RF) in 19, IgG4-related kidney disease (IgG4-KD) in 6, IgG4-related pseudotumor (IgG4-PT) in 3. Sixty-five patients (49%) had multiple IgG4-RD (two affected organs in 36 patients, three in 19, four in 8, five in 1, and six in 1). Serum IgG4 levels were significantly higher with multiple lesions than with a single lesion (P<0.001). The proportion of association with other IgG4-RD was 42% in AIP, the lowest of all IgG4-RDs. Serum IgG4 level was lower in AIP than in other IgG4-RDs. Frequently associated IgG4-RDs were SIA (25%) and DAC (12%) for AIP; AIP (75%) for IgG4-SC; DAC (57%), AIP (38%) and LYM (27%) for IgG4-SIA; AIP (26%) and LYM (26%) for IgG4-DAC; SIA (75%), DAC (50%) and AIP (45%) for IgG4-LYM; SIA (58%), AIP (42%) and LYM (32%) for IgG4-RF; AIP (100%) and SIA (67%) for IgG4-KID; and DAC (67%) and SIA (67%) for IgG4-PT. Most associated IgG4-RD lesions were diagnosed simultaneously, but IgG4-SIA and IgG4-DAC were sometimes identified before other lesions. About half of IgG4-RD patients had multiple IgG4-RD lesions, and some associations were seen between specific organs.


Subject(s)
Adult , Aged , Autoimmune Diseases/epidemiology , Comorbidity , Female , Humans , Immunoglobulin G/immunology , Japan/epidemiology , Male , Middle Aged , Multiple Organ Failure/epidemiology , Organ Specificity/immunology , Prevalence , Risk Factors , Statistics as Topic
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