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1.
Braz. j. biol ; 83: e249158, 2023. graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1339346

ABSTRACT

Abstract The knowledge of the testicular and ovarian morphology of a particular fish species is of paramount importance. Such analyze enables the development of studies and techniques aiming the improvement of their reproduction, management, commercialization and even their conservation. This study performed the ovarian and testicular characterization of the ornamental Amazon fish Serrapinnus kriegi. A total of three males and three females had their gonads analyzed by optical microscopy. Females present ovaries filled with oocytes in asynchronous development, indicating partial spawning in the species. Moreover, the micropyle and micropilar cell formation was observed in primary growing oocytes, representing a precocious oocyte development; and the zona radiata in the final vitellogenic oocytes is thicker than other related species, evidencing the development of a better protection to the embryos in function of the waters' turbulence that characterize it spawning sites in the Amazonian streams. The male specimens' present anastomosed tubular testes with unrestricted spermatogonia spread along the entire seminiferous tubules. The present data elucidate the dynamic of spermatogenesis and oogenesis of an ornamental Amazonian species, through the description of the male and female germ cells development.


Resumo O conhecimento da morfologia testicular e ovariana de uma determinada espécie de peixe é de suma importância, pois através destas análises é possível o desenvolvimento de estudos e técnicas visando o melhoramento de sua reprodução, manejo e comercialização e até mesmo auxiliar em sua conservação. Este estudo realizou a caracterização ovariana e testicular do peixe Amazônico ornamental Serrapinnus kriegi. Um total de três machos e três fêmeas tiveram suas gônadas analisadas através de microscopia óptica. As fêmeas apresentam ovários preenchidos por oócitos em desenvolvimento assincrônico, indicando desova parcelada da espécie. Além disso, observou-se a formação de micrópila e célula micropilar em oócitos em crescimento primário, representando o desenvolvimento precoce do oócito; a zona radiata nos oócitos vitelogênicos finais é mais espessa em comparação a outras espécies relacionadas, evidenciando o desenvolvimento de uma melhor proteção aos embriões, em função das águas turbulentas que caracterizam seu local de desova nos córregos amazônicos. Os machos apresentam testículos do tipo tubular anastomosado com espermatogônias irrestritas, espalhadas por todo o túbulo seminífero. Os dados apresentados elucidam a dinâmica da espermatogênese e oogênese de uma espécie de peixe ornamental amazônica, por meio da descrição das células germinativas masculinas e femininas.


Subject(s)
Animals , Male , Female , Characidae , Oocytes , Oogenesis , Ovary , Testis , Gonads
2.
Alerta (San Salvador) ; 5(1): 6-11, ene. 28, 2022. ilus
Article in Spanish | LILACS, BISSAL | ID: biblio-1354292

ABSTRACT

El quiste paraovárico se desarrolla en el ligamento ancho, entre la trompa de Falopio y el ovario. Son estructuras redondeadas de pared lisa, llenas de líquido seroso, que se ubican en el mesosálpinx, el ligamento ancho o sobre la propia trompa uterina. Tienen una incidencia aproximada de 1 en 1 500 0002 son frecuentes en la tercera y cuarta década de la vida; raramente se presentan en la edad pediátrica. Generalmente, son benignos y tiene un crecimiento lento y progresivo, suelen ser asintomáticos por lo que el diagnóstico suele ser incidental. En casos de quistes de gran volumen, los síntomas son dolor pélvico o la tumoración ab-dominal. Usualmente son diagnosticados clínicamente y se confirman por imágenes ultrasonográficas. La importancia de este caso es la poca frecuencia de estos tumores en la edad pediátrica y su identificación oportuna por parte de los profesionales de la salud


The paraovarian cyst develops in the broad ligament, between the fallopian tube and the ovary. They are rounded structures with a smooth wall, filled with serous fluid, which are located in the mesosalpinx, the broad ligament or on the uterine tube itself. They have an approximate incidence of 1 in 1,500,0002 and are common in the third and fourth decades of life; they rarely occur in childhood. Generally, they are benign and have a slow and progressive growth, they are usually asymptomatic, so the diagnosis is usually incidental. In cases of large volume cysts, the symptoms are pelvic pain or abdominal mass. They are usually diagnosed clinically and confirmed by ultrasonographic images. The importance of this case is the infrequency of these tumors in the pediatric age and their timely identification by health professionals


Subject(s)
Parovarian Cyst , Pediatrics , Diagnosis , Ovary , Broad Ligament , Pelvic Pain
3.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 24(2, cont.): e2403, jul-dez. 2021. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1348228

ABSTRACT

O procedimento cirúrgico eletivo realizado com maior frequência na rotina veterinária é a ovário-histerectomia (OVH), com isso há uma alta casuística de complicações pós-operatórias. Entre elas, pode-se citar piometra de coto, ligadura acidental de ureter, síndrome do ovário remanescente, formação de granulomas inflamatórios e aderências a órgãos. Os granulomas de coto ovariano são formados, geralmente por um fio de sutura utilizado inadequadamente, ou a um sítio de infecção. Tais granulomas podem levar à aderências, inclusive a órgãos próximos ao sítio cirúrgico. O presente relato refere-se a uma cadela, que apresentou granulomas ovarianos com aderências no pâncreas e em diversos órgãos após a ovário-histerectomia. Para a solução do caso, foi realizado uma celiotomia exploratória para avaliação da cavidade abdominal, retirada das aderências das estruturas e das formações granulomatosas, sendo necessária a realização de uma pancreatectomia parcial para a retirada completa do granuloma. Após a cirurgia, o paciente desenvolveu quadro de pancreatite, uma complicação muito comum em cirurgias pancreáticas. Ainda assim, o resultado foi considerado satisfatório, pois a pancreatite foi solucionada e os granulomas removidos, e até a última revisão pós-operatória, o paciente encontrava-se bem clinicamente. A OVH é uma das cirurgias mais realizadas na rotina médico veterinária, ainda assim é negligenciada em diversos quesitos. Portanto, há a necessidade dessa técnica cirúrgica e suas possíveis complicações serem realizadas de maneira correta e efetiva.(AU)


The most frequently elective surgical procedure performed in the veterinary routine is the ovary hysterectomy (OVH). As such, there is a high number of post-operative complications, which include stump pyometra, accidental ligation of the ureter, remnant ovary syndrome, formation of inflammatory granulomas, and organ adhesions. Ovarian stump granulomas are usually formed by suture which has been improperly used, or to infection. Such granulomas can lead to adhesions, also affecting organs close to the surgical site. This report refers to a female dog that presented ovarian granulomas with adhesions to the pancreas and to several organs after ovary-hysterectomy. In order to solve the case, an exploratory celiotomy was performed to assess the abdominal cavity, removing the adhesions of the structures and granulomatous formations, which required the performance of a partial pancreatectomy for the thorough removal of the granuloma. After surgery, the patient developed pancreatitis, a very common complication in pancreatic surgery. Even so, the result was considered satisfactory, as the pancreatitis was resolved and the granulomas removed and until the last post-operative review, the patient was clinically well. OVH is one of the most commonly performed surgeries in the veterinary medical routine, yet it is neglected in several aspects. Therefore, there is a need for the surgical technique and its possible complications to be performed correctly and effectively.(AU)


El procedimiento quirúrgico electivo que se realiza con mayor frecuencia en la rutina veterinaria es la ovario-histerectomía (OVH), con que existe un elevado número de complicaciones postoperatorias. Estos incluyen piometra del muñón, ligadura accidental del uréter, síndrome de ovario remanente, formación de granulomas inflamatorios y adherencias a órganos. Los granulomas del muñón ovárico se forman por lo general por una sutura mal utilizada o en un sitio de infección. Dichos granulomas pueden provocar adherencias, incluso a órganos cercanos al sitio quirúrgico. El presente informe se refiere a una perra, quien presentó granulomas ováricos con adherencias en el páncreas y en varios órganos tras ovario histerectomía. Para la solución del caso se realizó una celiotomía exploratoria para acceder la cavidad abdominal, removiendo las adherencias de las estructuras y formaciones granulomatosas, requiriendo la realización de una pancreatectomía parcial para la extirpación completa del granuloma. Después de la cirugía, la paciente desarrolló pancreatitis, una complicación muy común en la cirugía pancreática. Aun así, el resultado se consideró satisfactorio, ya que se resolvió la pancreatitis y se retiraron los granulomas, y hasta la última revisión postoperatoria la paciente se encontraba clínicamente bien. La OVH es una de las cirugías que se realizan con mayor frecuencia en la rutina médica veterinaria, pero se descuida en varios aspectos. Por tanto, existe la necesidad de que esta técnica quirúrgica y sus posibles complicaciones se realicen de forma correcta y eficaz.(AU)


Subject(s)
Animals , Female , Dogs , Ovary , Pancreatectomy , Postoperative Complications , Elective Surgical Procedures , Dogs/surgery , Granuloma , Hysterectomy
4.
Arq. Ciênc. Vet. Zool. UNIPAR (Online) ; 24(2, cont.): e2405, jul-dez. 2021. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1352310

ABSTRACT

A castração em fêmeas pode ser feita pela ovariectomia (retirada dos ovários), ou ovariohisterectomia (retirada dos ovários e do útero). Essa última técnica é a mais recomendada em cães e gatos, devido à prevenção de afecções que podem ocorrer posteriormente no útero. Dentre essas complicações pode-se encontrar sangramento por ligadura mal feita em coto, hidroureter, hidronefrose, piometra de coto, formação de tratos sinusais, aderência, obstrução crônica, incontinência urinária, retenção abdominal de compressas cirúrgicas, transcecção de parte do ureter e ovário remanescente. Em cadelas castradas, a causa mais comum da formação de tratos sinusais é a presença de reação tecidual ao redor do fio de sutura. O presente estudo teve como objetivo relatar um caso de uma cadela sem raça definida, 8,7 kg, seis anos, que deu entrada no Hospital Veterinário de Uberaba, MG com queixa principal a presença de ferida no flanco direito sendo tratada há nove meses, porém sem sucesso. Já haviam feito uso de vários antibióticos sistêmicos e antinflamatorios esteroidais e não esteroidais. Diante do caso observado, foram realizados exames laboratoriais e de imagem. Perante o resultado do exame de imagem, a principal suspeita foi a formação de trato sinusal com comunicação com a cavidade abdominal devido a uma reação ao fio de algodão utilizado na ovariohisterectomia.(AU)


Female animals can be castrated by ovariectomy (removal of the ovaries), or ovariohysterectomy (removal of the ovaries and uterus). The latter is the most recommended in dogs and cats due to the prevention of conditions that may subsequently occur in the uterus. Among these complications, one can find bleeding by poor ligation in the stump, hydroureter, hydronephrosis, stump pyometra, formation of sinus tracts, adherence, chronic obstruction, urinary incontinence, abdominal retention of surgical compresses, transection of part of the ureter and remaining ovary. In castrated bitches, the most common cause of the formation of sinus tracts is the presence of tissue reaction around the suture thread. This study aimed at reporting a case of a mixed breed, six-year-old dog, 8.7 kg that was admitted to the Veterinary Hospital of Uberaba with the main complaint of the presence of a wound on the right flank being unsuccessfully treated for nine months. Several systemic antibiotics and steroidal and non-steroidal anti-inflammatory drugs had already been used. In view of the observed case, laboratory and imaging exams were performed. In view of the result of the imaging examination, the main suspicion was the formation of a sinus tract with communication to the abdominal cavity due to a reaction to the cotton thread used in the ovariohysterectomy.(AU)


La castración en hembras se puede realizar mediante ovariectomía (extirpación de los ovarios) u ovario histerectomía (extirpación de los ovarios y del útero). Esa última técnica es la más recomendada en perros y gatos, debido a la prevención de afecciones que pueden presentarse posteriormente en el útero. Entre estas complicaciones se puede encontrar hemorragias por ligadura mal realizada en muñón, hidruréter, hidronefrosis, piometra de muñón, formación de tractos sinusales, adherencia, obstrucción crónica, incontinencia urinaria, retención abdominal de compresas quirúrgicas, transección de parte del uréter y ovario remanente. En las perras castradas, la causa más común de formación de trayectos sinusales es la presencia de reacción tisular alrededor del hilo de sutura. El presente estudio tuvo como objetivo reportar el caso de una perra sin raza definida, de 8,7 kg, de seis años de edad, que ingresó en el Hospital Veterinario de Uberaba, MG con el principal síntoma de presencia de una herida en el flanco derecho siendo tratada durante nueve meses, pero sin éxito. Ya habían usado varios antibióticos sistémicos y medicamentos antiinflamatorios esteroides y no esteroides. A la vista del caso observado, se realizaron exámenes de laboratorio y de imagen. A la vista del resultado del examen de imagen, la principal sospecha fue la formación de un tracto sinusal con comunicación con la cavidad abdominal debido a una reacción al hilo de algodón utilizado en el ovario histerectomía.(AU)


Subject(s)
Animals , Female , Dogs , Ovary , Sutures , Ovariectomy , Castration , Dogs/surgery , Pyometra , Anti-Bacterial Agents
5.
Arq. bras. med. vet. zootec. (Online) ; 73(3): 653-657, May-June 2021. ilus
Article in English | LILACS, VETINDEX | ID: biblio-1278359

ABSTRACT

Squamous metaplasia of the rete ovarii is an ovarian pathologic change characterized by replacement of the normal single layered cuboidal epithelium of the rete ovarii by a stratified squamous keratinized epithelium. Uterus and ovaries from a local slaughterhouse pregnant crossbreed cow were evaluated through ultrasound, macroscopically and histologically. Grossly, there were multiple cysts in both ovaries, which were histologically characterized as rete ovarii cysts with squamous metaplasia and intraluminal accumulation of keratinized material. Squamous metaplasia of the rete ovarii has been previously reported in cows, however this is the first report of this condition in a pregnant animal, demonstrating that this ovarian change is compatible with pregnancy.(AU)


A metaplasia escamosa da rete ovarii é uma patologia ovariana caracterizada pela substituição do epitélio simples cuboidal normal da rete ovarii por um epitélio estratificado escamoso queratinizado. Útero e ovários de uma vaca mestiça gestante, proveniente de abatedouro, foram avaliados por ultrassonografia, macroscopia e histologia. Verificaram-se vários cistos em ambos os ovários, histologicamente caracterizados como cistos de rete ovarii com metaplasia escamosa, com acúmulo intraluminal de material queratinizado. Metaplasia escamosa da rete ovarii foi relatada anteriormente em vacas, porém este é o primeiro relato em que essa alteração ovariana é compatível com manutenção da ciclicidde ovariana e gestação na vaca.(AU)


Subject(s)
Animals , Female , Pregnancy , Cattle , Ovary/pathology , Teratoma/veterinary , Pregnancy, Animal/physiology , Estrous Cycle/physiology , Epidermal Cyst/veterinary , Epithelium/pathology , Metaplasia/veterinary
6.
Article in Chinese | WPRIM | ID: wpr-921809

ABSTRACT

The present study investigated the effect of Modified Dihuang Decoction in improving ovarian reserve in mice through the Bcl-2-related mitochondrial apoptosis pathway. Forty-eight adult female BALB/c mice were randomly divided into the following six groups with eight mice in each group: a blank group, a model group, a femoston group(three cycles of treatment with 0.13 mg·kg~(-1) estradiol tablets for 2 days and 1.43 mg·kg~(-1) estradiol and dydrogesterone tablets for 3 days), and high(64.74 g·kg~(-1))-, medium(43.16 g·kg~(-1))-, and low-dose(21.58 g·kg~(-1)) Modified Dihuang Decoction groups. Mice in other groups except the blank group received a single intraperitoneal injection of 12 mg·kg~(-1) cyclophosphamide and 1.2 mg·kg~(-1) busulfan to induce a model of diminished ovarian reserve(DOR), while those in the blank group received an equal volume of normal saline. Mice were treated with corresponding drugs for 15 d from the 36 th day, once per day, and the mice in the blank group and the model group were treated with an equal volume of normal saline. The general condition and oestrous cycle were observed. The serum hormone levels were detected with the enzyme-linked immunosorbent assay(ELISA). The morphological changes of ovaries were observed by HE staining. Western blot was used to detect the protein expression of cysteinyl aspartate specific proteinase-9(caspase-9), cleaved caspase-3, Bcl-2 associated X protein(Bax), Bcl-2, superoxide dismutase-2(SOD-2), and glutathione peroxidase-1(GPx-1). The mRNA expression of Bax and Bcl-2 was detected by real-time fluorescence-based quantitative polymerase chain reaction(real-time PCR). The results showed that compared with the blank group, the model group showed body weight loss, disordered oestrous cycle, elevated serum levels of follicle-stimulating hormone(FSH) and luteinizing hormone(LH), reduced serum levels of estradiol(E_2), anti-mullerian hormone(AMH), and inhibin B(INHB), the declining number of ovarian follicles and granulosa layers, increased number of atretic follicles, up-regulated protein expression of caspase-9, cleaved caspase-3, and Bax and Bax mRNA expression in ovaries, and down-regulated protein expression of Bcl-2, SOD-2 and GPx-1, and Bcl-2 mRNA expression. Compared with the model group, the Modified Dihuang Decoction groups displayed restored body weight and oestrous cycle, decreased serum levels of FSH and LH, elevated serum levels of E_2, AMH, and INHB, increased number of ovarian follicles, thickened granulosa layers, and declining number of atretic follicles. Additionally, the protein expression of caspase-9, cleaved caspase-3, and Bax, and Bax mRNA expression was down-regulated, and the protein expression of Bcl-2, SOD-2, and GPx-1, and Bcl-2 mRNA expression was up-regulated. The results suggest that Modified Dihuang Decoction can regulate endocrine hormone, promote follicle growth and improve ovarian reserve by enhancing ovarian anti-oxidant capacity, inhibiting the Bcl-2-related mitochondrial apoptosis pathway, and further inhibiting cell apoptosis.


Subject(s)
Animals , Apoptosis , Female , Mice , Mice, Inbred BALB C , Ovarian Follicle , Ovarian Reserve , Ovary
7.
Article in Chinese | WPRIM | ID: wpr-879041

ABSTRACT

Protein kinase C(PKC) is a kind of kinase which is widely involved in cell proliferation and development. PKC(Wp-PKC) in Whitmania pigra body belongs to classic PKC. In order to investigate the effect of Wp-PKC on the development of Wh. pigra germ cells, 17β-estradiol(17β-E2)(100 ng·mL~(-1)) and methyltestosterone(MT)(150 μg·L~(-1)), 150 μg·L~(-1)(MT)+0.5 mg·L~(-1) PKC, 0.5 mg·L~(-1) PKC inhibitor were added to Wh. pigra culture water, and no addition group(control group) was added, and the effects on the development of Wh. pigra germ cells and the expression of Wp-PKC were observed. The results showed that: Wp-PKC in male gonads was always higher than that in female gonads; MT promoted the development of male gonads in Wh. pigra, while the expression of Wp-PKC was significantly higher than that in the control; 17β-E2 promoted the development of female gonads in Wh. pigra and Wp-PKC expression significantly lower than that of the control; while the development of the female and male gonads in the PKC inhibitor group was inhibited, the expression of Wp-PKC was significantly lower than that of the control. In summary, Wp-PKC may promote the development of Wh. pigra, especially the development of male gonads.


Subject(s)
Animals , Estradiol , Female , Gonads , Leeches , Male , Methyltestosterone , Ovary
8.
Article in Chinese | WPRIM | ID: wpr-878884

ABSTRACT

Protein kinase C(PKC) is a type of protein kinase widely involved in cell proliferation and development, but the developmental mechanism in the gonads of androgynous animals is still unclear. In order to explore the role of protein kinase C in the development of Whitmania pigra germ cells, the Wh. pigra PKC(Wp-PKC) gene was cloned, bioinformatics analysis was conducted, and fluorescent quantitative PCR was used to analyze the expression of female and male gonads. The results showed that:(1)The cloned Wp-PKC had a full length of 2 580 bp, a relative molecular weight of 76 555.19, and contains an open reading frame encoding 670 amino acids, Wp-PKC was closely related to Danio rerio PKC-α and rat PKC-γ. The similarity of amino acid sequence was 55% and 58%.(2)The protein encoded by Wp-PKC had no signal peptide and was a hydrophilic protein. The secondary structure is mainly composed of random coils, α-helices, extended chains, folds and folds, with the largest proportion of random coils and α-helices. Wp-PKC protein does not contain a transmembrane domain. Multiple sequence alignment and domain prediction analysis show that Wp-PKC contains 4 conserved domains of classical protein kinase C.(3)Fluorescence quantitative results showed that the expression of Wp-PKC in Wh. pigra gonads was positively correlated with the development of germ cells, and the expression in male gonads was significantly higher than that in female gonads. In summary, Wp-PKC is a classic PKC, and Wp-PKC may promote the development of Wh. pigra, especially the development of male gonads, and provide references for further research on the developmental mechanisms of Wh. pigra.


Subject(s)
Animals , Cloning, Molecular , Female , Gonads , Leeches/genetics , Male , Ovary , Protein Kinase C/genetics , Rats
9.
Article in Chinese | WPRIM | ID: wpr-878719

ABSTRACT

Objective To investigate the effects of self-made carriers on the cryopreservation of ovarian tissue of sheep. Methods Thirty-two ovaries were randomly assigned to fresh group,programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group.The morphology,proliferation,apoptosis,and estrogen level of the ovarian tissue in each group were observed. Results After cryopreservation,the morphology normal rate of the primordial follicles in programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group were 74.2%,72.8%,and 72.3%,respectively,lower than that(83.7%)in the fresh group(χ


Subject(s)
Animals , Cryopreservation , Female , Freezing , Ovarian Follicle , Ovary , Sheep , Vitrification
10.
Arq. bras. med. vet. zootec. (Online) ; 72(6): 2101-2110, Nov.-Dec. 2020. tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1142286

ABSTRACT

O conhecimento da anatomia de qualquer animal silvestre é de fundamental importância para sua preservação e proteção. Neste contexto, o presente estudo objetivou descrever a morfologia do sistema reprodutor feminino de Alouatta belzebul. Foram utilizados seis espécimes de A. belzebul, fêmeas, adultas, e livres de lesões. Observou-se macroscopicamente que os ovários têm características morfológicas em formato ovoides, com superfície lisa, e, na análise histológica na região de córtex, evidenciou-se folículos ovarianos em diferentes estágios de desenvolvimento. As tubas uterinas anatomicamente são finas e curvilíneas, apresentando uma camada mucosa, uma muscular e outra serosa. O útero possui formato simples, com fundo globoso, com um miométrio altamente vascularizado, sendo organizado em feixes de fibras musculares lisas. A estrutura anatômica da vagina apresentou-se como um tubo muscular longo de paredes finas, onde, na região vestibular, o óstio externo da uretra é marcado por uma papila uretral bilobada e, na região de vulva, em sua porção caudal, contatou-se um clitóris bem desenvolvido. No que concerne à análise histológica da vagina, verificou-se, em região de mucosa vaginal, um extrato basal composto por epitélio estratificado pavimentoso não queratinizado atrófico. As descrições morfológicas fornecem, de forma inédita, informações importantes relativas à anatomia macroscópica e microscópica do sistema reprodutor feminino dessa espécie.(AU)


Knowledge of the anatomy of any wild animal is of fundamental importance for its preservation and protection. In this context the present study aimed to describe the morphology of the female reproductive system of A. belzebul. We used 6 specimens of A. belzebul, female, adult and free of lesions. It was macroscopically observed that the ovaries are ovoid with smooth surface and the histological analysis in cortical region showed ovarian follicles in different stages of development. The fallopian tubes are anatomically thin and curvilinear, with one mucous layer, one muscular and one serous layer. The uterus was presented in a simple format with a globular fundus, with a highly vascularized myometrium, being organized in bundles of smooth muscle fibers. The anatomical structure of the vagina presented itself as a long thin-walled muscular tube where in the vestibular region the external orifice of the urethra is marked by a bilobed urethral papilla and in the caudal portion in its caudal portion a well-developed clitoris. Regarding the histological analysis of the vagina, a basal extract composed of atrophic non-keratinized stratified squamous epithelium was found in the vaginal mucosa region. The morphological descriptions provide important information regarding the macroscopic and microscopic anatomy of the female reproductive system of this species in an unprecedented way.(AU)


Subject(s)
Animals , Female , Ovary/anatomy & histology , Uterus/anatomy & histology , Vagina/anatomy & histology , Alouatta/anatomy & histology , Fallopian Tubes/anatomy & histology , Genitalia, Female/anatomy & histology
11.
Int. j. morphol ; 38(5): 1405-1411, oct. 2020. graf
Article in English | LILACS | ID: biblio-1134456

ABSTRACT

SUMMARY: Establishing guidelines for responsible management in fish production systems requires knowledge of the basic biology of the fish to be raised. The objective of this work was to determine the reproductive capacity of hybrids produced from the crossing of females of Pseudoplatystoma metaense with males of Leiarius marmoratus. Males presented a digitiform, unrestricted spermatogonial testicle containing caudal digits producing glycoproteins that do not form a seminal vesicle. It was possible to find free sperm in the lumen of the tubules and in the ducts. The ovary of the females was found to be saccular and synchronous with at least three groups of oocytes. In the first year of life, only oogonia up to previtellogenic oocytes (cortical alveolus 284.9 ± 35.7 mm in diameter) were found. After the second year vitellogenic oocytes 730 ± 3.78 mm in diameter were observed. The events of gonadal development of the hybrids indicate that they are gonochoric, synchronic animals. The maturation peaks in the high-water season, overlapping with the parental species. Therefore, the escape of hybrids from fish cultures to the rivers may increase the risk of crosses, gene introgression, or diminution of the reproductive capacity of the pure species.


RESUMEN: El establecimiento de pautas para la gestión responsable en los sistemas de producción de peces requiere el conocimiento de la biología básica de los peces a criar. El objetivo de este trabajo fue determinar la capacidad reproductiva de los híbridos producidos por el cruce de hembras de Pseudoplatystoma metaense con machos de Leiarius marmoratus. Los machos presentaron un testículo espermatogonial digital no restringido que contiene dígitos caudales que producen glucoproteínas que no forman una vesícula seminal. Fue posible encontrar esperma libre en la luz de los túbulos y en los conductos. Se encontró que el ovario de las hembras era sacular y sincrónico con al menos tres grupos de ovocitos. En el primer año de vida, solo se encontraron oogonia hasta ovocitos previtelogénicos (alvéolo cortical de 284,9 ± 35,7 mm de diámetro). Después del segundo año, se observaron ovocitos vitelogénicos de 730 ± 3,78 mm de diámetro. Los eventos de desarrollo gonadal de los híbridos indican que son animales sincrónicos gonocóricos. La maduración alcanza su punto máximo en la temporada de aguas altas, superponiéndose con las especies parentales. Por lo tanto, el escape de híbridos de cultivos de peces a los ríos puede aumentar el riesgo de cruces, introgresión genética o disminución de la capacidad reproductiva de las especies puras.


Subject(s)
Animals , Male , Female , Sexual Maturation , Catfishes , Gonads/growth & development , Oocytes , Ovary/anatomy & histology , Ovary/growth & development , Testis/anatomy & histology , Testis/growth & development , Gonads/anatomy & histology
12.
Rev. cuba. med. mil ; 49(3): e457, jul.-set. 2020. fig
Article in Spanish | LILACS, CUMED | ID: biblio-1144487

ABSTRACT

Introducción: Los tumores de gran tamaño han sido descritos a través de la historia, entre ellos los ginecológicos y los de ovario cuando pesan más de 12 kg constituyen una rareza médica. Las masas tumorales en hemiabdomen inferior son causas frecuentes de consulta en cirugía general. Dentro de estas, los quistes de ovarios son los que con más frecuencia se diagnostican. Objetivo: Describir el caso de un quiste gigante de ovario en una paciente que fue atendida en consulta de cirugia general por aumento de tamaño en el abdomen de varios años de evolución. Caso clínico: Se expone el caso de una paciente femenina, con antecedentes de salud, que acude al servicio de cirugía general por aumento de volumen del abdomen, progresivo, insidioso de 2 años de evolución. Se diagnostica masa quística dependiente de ovario y en el transoperatorio se constata una tumoración quística del ovario que en el análisis histopatológico informa un cistoadenoma seroso de ovario. Conclusión: Los tumores del ovario no son tan frecuentes como los del útero y los de la mama, pero constituyen el tercer grupo de tumores benignos y malignos de la mujer. Se presentó el caso por lo infrecuente que resulta, la escasa frecuencia de reporte de estos casos lo cual aporta conocimiento a la comunidad médica sobre el tema(AU)


Introduction: Large tumors have been described throughout history, including gynecological tumors, and ovarian tumors when they weigh more than 12 kg constitute a medical rarity. Tumor masses in lower hemiabdomen are frequent causes of consultation in general surgery. Within these, ovarian cysts are the most frequently diagnosed. Objective: To describe the case of a giant ovarian cyst in a patient who was treated in a general surgery consultation due to an increase in abdomen size of several years of evolution. Clinical case: The case of a female patient, with a history of health, which goes to the general surgery service due to an increase in abdomen volume, progressive, insidious of 2 years of evolution, is presented. Ovarian-dependent cystic mass is diagnosed and a cystic tumor of the ovary is found in the transoperative period, which in the histopathological analysis reports a serous ovarian cystadenoma. Conclusion: Ovarian tumors are not as frequent as those of the uterus and those of the breast, but they constitute the third group of benign and malignant tumors of women. The case was presented because of the infrequent result, the low frequency of reporting these cases, which brings knowledge to the medical community on the subject(AU)


Subject(s)
Humans , Female , Middle Aged , Ovarian Cysts/surgery , Ovary/diagnostic imaging , Uterus , Abdomen
13.
Oncol. (Guayaquil) ; 30(2): 106-122, 31 de agosto del 2020.
Article in Spanish | LILACS | ID: biblio-1141454

ABSTRACT

Introducción:El cáncer avanzado de ovario es una de las enfermedades oncológicas de mayor mortalidad en el mundo. En el Ecuador representa aproximadamente el 0.2% de todas las muertes. Elobjetivo de del estudio fue realizar una descripción clínica retrospectiva de las mujeres con cáncer avanzado de ovario. Métodos:El presente estudio observacional, retrospectivo fue realizado en el servicio de Oncología Clínica del Instituto Oncológico Nacional "Dr. Juan Tanca Marengo" de Solca-Guayaquil.El período de estudio fue enero del 2003 al diciembre del 2007. Se incluyeron los registros de pacientes con diagnostico histopatológico del tipo de cáncer avanzado de ovario: con estadificación según FIGO III (IIIA-IIIB-IIIC) y IV. Las variables fueron descriptivas poblacionalesy clínicas: tipo histológico, estadificación, tratamiento empleado. El cálculo de la muestra es no probabilística, en la que se incluyen todos los casos posibles. Se utiliza estadística descriptiva. Resultados:79 casos ingresaron al análisis. Las mujeres de 50 a 59 años20/79 casos 25.32 (IC95%24.24-26.4%). El Adenocarcinomapapilar seroso IIIcfue la histología más prevalente 34/79 (43.04 % IC95% 41.81-44.27%). El tratamientoquimioterápico con cirugía fue el más prevalente 56/79 (70.89% IC95% 69.76-72.01). Se usó Paclitaxel+Cisplatinoen 44/79casos(55.7% IC95% 54.46-56.93%). La recidiva se presentó en 30 casos. Respuesta completa 17 casos (21.52% IC95% 20.5-22.54%), progresión en 18casos (22.78% IC95% 21.74-23.83%).Mortalidad 11 casos (13.92% IC95% 13.07-14.78%)y abandono de tratamiento32casos(40.51% IC95%39.29-41.72%). Conclusiones: La tasa de abandono es un factor que se presenta con una alta frecuenciaentre los pacientes con cáncer avanzado de ovario. Esto no permite evaluar el resultado de los tratamientos que son utilizadas para tratar los cánceres de ovario en estas etapas.


Introduction:Advanced ovarian cancer is one of the cancer diseases with the highest mortality in the world. In Ecuador it represents approximately 0.2% of all deaths. The objective of the study was to carry outa retrospective clinical description of women with advanced ovarian cancer. Methods: The present observational, retrospective study was carried out in the Clinical Oncology service of the National Oncological Institute "Dr. Juan Tanca Marengo"from Solca-Guayaquil. The study period was January 2003 to December 2007. The records of patients with histopathological diagnosis of advanced ovarian cancer were included: staging according to FIGO III (IIIA-IIIB-IIIC) and IV. The variables were descriptive, population and clinical: histological type, staging, treatment used. The calculation of the sample is non-probabilistic, which includes all possible cases. Descriptive statistics are used. Results: 79 cases entered the analysis. Women aged 50 to 59 years 20/79 cases 25.32 (95% CI 24.24-26.4%). Serous papillary adenocarcinoma IIIc was the most prevalent histology 34/79 (43.04% 95% CI 41.81-44.27%). Chemotherapy treatment with surgery was the most prevalent 56/79 (70.89% 95% CI 69.76-72.01). Paclitaxel + Cisplatin was used in 44/79 cases (55.7% 95% CI 54.46-56.93%). Recurrence occurred in 30 cases. Complete response in 17 cases (21.52% 95% CI 20.5-22.54%), progression in 18 cases (22.78% 95% CI 21.74-23.83%). Mortality 11 cases (13.92% 95% CI 13.07-14.78%) and treatment abandonment 32 cases (40.51% 95% CI 39.29-41.72%). Conclusions: The dropout rate is a factor that occurs with a high frequency among patients with advanced ovarian cancer. This does not allow evaluating the outcome of the treatments that are used to treat ovarian cancers at these stages


Subject(s)
Humans , Ovarian Neoplasms , Mortality , Drug Therapy , Ovary , Treatment Adherence and Compliance
14.
Semina cienc. biol. saude ; 41(2, Supl.): 389-402, jun./dez. 2020. Ilus
Article in Portuguese | LILACS | ID: biblio-1247563

ABSTRACT

Os herbicidas estão entre os insumos químicos mais utilizados na agricultura e apresentam ação toxicológica para as plantas. Dentre os herbicidas existentes, o glifosato e a sua formulação comercial Roundup® são os mais utilizados devido à eficácia no controle de ervas daninhas. Entretanto, estudos sugerem que estes herbicidas podem provocar distúrbios a organismos aquáticos, principalmente o Roundup®, que além do glifosato ativo, apresenta na composição componentes surfactantes que aumentariam o seu potencial toxicológico para os seres vivos. Desta forma, o presente estudo teve como objetivo comparar o potencial mutagênico e desenvolvimento gonadal do princípio ativo glifosato e sua formulação Roundup® em peixes Danio rerio. Para isso, exemplares adultos de D. rerio permaneceram em aquários expostos à concentração de 65 µg/L de cada herbicida. Foram realizadas contagens de micronúcleos, calculado o índice gonadossomático (IGS) e verificado os estágios de maturação gonadal. Os resultados demonstraram que os peixes expostos aos herbicidas apresentaram aumentos significativos na frequência de micronúcleos, bem como no IGS, e maior ocorrência de ovários com estágios em maturação e maduros. Não foram verificadas diferenças significativas na comparação dos herbicidas, exceto um aumento na frequência de ovários maduros nas fêmeas expostas ao glifosato. Observou-se que, mesmo em pequenas concentrações, os herbicidas poderiam ter ocasionado efeitos nocivos para os peixes D. rerio, podendo induzir efeitos clastogênicos nos eritrócitos, assim como distúrbios reprodutivos, aumentando a preocupação sobre os impactos que estes herbicidas podem acarretar sobre o ciclo de vida destes organismos.(AU)


The herbicides are among the most used chemical inputs in the agriculture and present a great toxicological action for the plants. Among the existing herbicides, glyphosate and its commercial formulation Roundup® are the most used due to their effectiveness in control of weeds. However, studies suggest that these herbicides can cause disturbances to aquatic organisms, mainly the Roundup®, that besides active glyphosate, it presents in the composition components surfactants that would increase its toxicological potential for the alive beings. Therefore, the aim of this study is to compare the mutagenic potential and gonadal development of the active principle glyphosate and its formulation Roundup® in fish Danio rerio. Thus, adult copies of D. rerio stayed in aquariums exposed to a concentration of 65 µg / L of each herbicide. Micronucleus counts were performed and the Gonadossomatic index (GSI) was calculated and the stages of gonadal maturation were verified. The results demonstrated that the fish exposed to the herbicides, presented significant increases in the micronucleus frequency, as well as in GSI and larger occurrence of ovaries with stages in maturation and mature. There were no significant differences in herbicide comparison, except an increase in the frequency of mature ovaries in the exposed females to glyphosate. It was observed that even in small concentrations, the herbicides could presented harmful effects for the fish D. rerio, could have caused clastogenic effects in the erythrocytes, as well as, reproductive disturbances, increasing the concern about the impacts that these herbicides can have on the life cycle of these organisms.(AU)


Subject(s)
Animals , Zebrafish , Fishes , Ovary , Agriculture , Weed Control
15.
Arch. argent. pediatr ; 118(3): e329-e332, jun. 2020. tab, ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-1117357

ABSTRACT

Los tumores de los cordones sexuales y estromales son neoplasias poco frecuentes, que corresponden al 8 % de los tumores primarios del ovario. El tumor de los cordones sexuales con túbulos anulares del ovario es considerado un subtipo y es infrecuente. Puede presentarse de manera esporádica o asociado al síndrome de Peutz-Jeghers y tiene diferente comportamiento y características en cada situación.Se presenta el caso de una paciente adolescente con diagnóstico de tumor de los cordones sexuales con túbulos anulares del ovario asociado a síndrome de Peutz-Jeghers


Tumors of the sexual and stromal cords are rare neoplasms, corresponding to 8 % of primary ovarian tumors. The tumor of the sexual cords with annular tubules of the ovary is considered a subtype and is uncommon. It can occur sporadically or associated with Peutz-Jeghers Syndrome, having different behavior and characteristics in each situation.We present the case of an adolescent patient with a diagnosis of a tumor of the sexual cords with annular tubules of the ovary associated with Peutz-Jeghers Syndrome


Subject(s)
Humans , Female , Adolescent , Peutz-Jeghers Syndrome , Sex Cord-Gonadal Stromal Tumors/diagnosis , Ovary/pathology , Sex Cord-Gonadal Stromal Tumors/surgery , Neoplasms
16.
Rev. chil. obstet. ginecol. (En línea) ; 85(3): 275-280, jun. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1126163

ABSTRACT

RESUMEN Presentamos caso clínico de puérpera de cesárea de noveno día con cuadro febril en espigas, asintomática, en que se plantea desde su ingreso el diagnóstico de tromboflebitis séptica pelviana y se inicia tratamiento con antibióticos parenterales de amplio espectro y heparina de bajo peso molecular en dosis terapéuticas. La respuesta es a la mejoría al tercer día de tratamiento. Se discuten los métodos diagnósticos, el diagnóstico diferencial y el tratamiento.


ABSTRACT A case of a woman on her 9th post-operative day after childbirth by cesarean section (CS) consulting with febrile spikes, otherwise asymptomatic, is presented. The diagnosis of a septic pelvic thrombophlebitis is proposed from the admission and treated with parenteral broad-spectrum antibiotics and low-molecular-weight heparin at therapeutic doses. Patient significantly improved on her third day of treatment. The diagnostic procedures, differential diagnosis and treatment are discussed.


Subject(s)
Humans , Female , Adult , Ovary/blood supply , Thrombophlebitis/etiology , Cesarean Section/adverse effects , Sepsis/etiology , Pelvis/blood supply , Thrombophlebitis/drug therapy , Thrombophlebitis/diagnostic imaging , Heparin/therapeutic use , Tomography, X-Ray Computed , Sepsis/drug therapy , Sepsis/diagnostic imaging , Diagnosis, Differential , Anti-Bacterial Agents/therapeutic use , Anticoagulants/therapeutic use
17.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 711-718, May-June, 2020. tab, graf
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1128882

ABSTRACT

Objetivou-se testar a vitrificação de ovários de camundongos do ICTB/Fiocruz. Inicialmente, fez-se coleta e maturação in vitro dos oócitos de ovários a fresco e vitrificados, bem como avaliação de estruturas no cultivo embrionário, pós-fertilização in vitro. Fêmeas B6D2F1 foram eutanasiadas para remoção dos ovários (n=60) e divididas em três grupos: grupo 1 (n=30 animais) - oócito de ovários vitrificados, maturados e fertilizados in vitro (120 fragmentos); grupo 2 (n=15) (controle 1) - oócitos coletados a fresco, maturados e fertilizados in vitro; e grupo 3 (n=15) (controle 2) - oócitos maturados in vivo e fertilizados in vitro. A técnica foi verificada no desenvolvimento embrionário in vitro, que foi avaliado pelo teste de qui-quadrado (BioStat 5.0). Recuperaram-se 123, 224 e 328 oócitos nos G1, G2 e G3, respectivamente. Observaram-se diferenças significativas nas taxas de clivagem às 24 horas (embriões ≥ 2 células) entre G1 (8%) e G2 (32%) (P<0,1) e G1 e G3 (49%) (P<0,05), mas não entre G2 e G3 (P>0,05). Para blastocistos, às 96 horas, os grupos G1, G2 e G3 apresentaram, respectivamente, 6%, 11% e 46%, diferindo significativamente entre eles (P<0,05). A vitrificação de ovários, a maturação oocitária e a fertilização in vitro são alternativas para a produção de embriões de camundongos in vitro.(AU)


This work aimed test ovarian vitrification of hybrid mouse from ICTB/Fiocruz. Protocol collection and oocyte in vitro maturation from fresh and vitrified ovaries was established and embryos were evaluated after fertilization. B6D2F1 females were euthanized for ovarian removal (n= 60) and divided into 3 groups: G1 (n= 30) - ovaries fragmented (n= 120), vitrified, matured and fertilized; G2 (n= 15) - in vitro fertilization of oocytes matured in vitro from fresh ovaries; G3 (n= 15) - ampulla region oocytes in vitro fertilizated. Viability was verified by thawing, oocyte in vitro maturation and fertilization. In vitro embryo development of each group was evaluated by Chi-square test (BioStat 5.0). 123, 224 and 328 oocytes were recovered from G1, G2 and G3, respectively. Significant differences were observed in cleavage rates at 24 hours (embryos with 2 cells or more) between G1 (8%) and G2 (32%) (P< 0.1) and G1 and G3 (49%) (P< 0.05) but not between G2 and G3 (P> 0.05). Blastocysts at 96 hours presented 6%, 11% and 46%, respectively for G1, G2 and G3, differing significantly (P< 0.05). Ovary vitrification, oocyte in vitro maturation and in vitro fertilization were available for the production of in vitro mouse embryos.(AU)


Subject(s)
Animals , Female , Mice , Ovary , Embryonic Development , Vitrification , Fertilization in Vitro , In Vitro Oocyte Maturation Techniques/veterinary
18.
Arq. bras. med. vet. zootec. (Online) ; 72(3): 895-900, May-June, 2020. ilus
Article in English | LILACS, VETINDEX | ID: biblio-1129565

ABSTRACT

An adult, female, 31kg body weight, free range Myrmecophaga tridactyla was referred for medical consultation due to apathy, dehydration, intense flatulence and fetid stools. The animal was submitted to chemical restraint and physical examination, blood count, fecal analysis, and vaginal cytology and abdominal ultrasonography were performed. Good nutritional status and clinical variables within the normal range were observed at physical examination. At vaginal cytology leukocytes, spermatozoa and a large proportion of cornified cells (superficial) were observed, indicating estrus and recent copula. At ultrasound examination it was possible to locate, identify, evaluate and measure the ovaries and the uterine structures, cervix, body, lumen, myometrium and endometrium, a fact never reported in the literature for this species. These data can be used as reference for clinical evaluation of the reproductive tract in Myrmecophaga tridactyla females considered vulnerable species, for the diagnosis of reproductive pathologies, biotechnologies application or estrous cycle evaluation.(AU)


Uma fêmea, com 31kg de peso corporal, de Myrmecophaga tridactyla, de vida livre, foi apresentada para consulta médica em razão de apatia, desidratação, flatulência intensa e fezes fétidas. O animal foi submetido à contenção química e foram realizados exame físico, hemograma, análise fecal, citologia vaginal e ultrassonografia abdominal. Ao exame físico, foi determinado bom estado nutricional e variáveis clínicas dentro da faixa de normalidade. Na citologia vaginal, foram observados leucócitos, espermatozoides e uma grande proporção de células cornificadas (superficiais), indicando estro e cópula recente. No exame ultrassonográfico, foi possível localizar, identificar, avaliar e mensurar os ovários e as estruturas uterinas, o colo do útero, o corpo do útero, o lúmen, o miométrio e o endométrio, fato nunca relatado na literatura para essa espécie. Esses dados podem ser utilizados como referência para avaliação clínica do trato reprodutivo de fêmeas de Myrmecophaga tridactyla considerada espécie vulnerável, para o diagnóstico de patologias reprodutivas, aplicação de biotecnologias ou avaliação do ciclo estral.(AU)


Subject(s)
Animals , Female , Ovary/diagnostic imaging , Uterus/diagnostic imaging , Cingulata/anatomy & histology , Genitalia/diagnostic imaging , Ultrasonography/veterinary , Estrous Cycle
19.
Pesqui. vet. bras ; 40(2): 141-154, Feb. 2020. tab, graf, ilus
Article in English | LILACS, VETINDEX | ID: biblio-1098447

ABSTRACT

Although Trachemys scripta elegans is an exotic species popular as a pet in Brazil, studies on reproductive biology and capacity are non-existent in the Brazilian Cerrado. This study analyzed ovarian and oviduct characteristics and the egg production capacity of T. scripta elegans grown in this biome. The findings will associate with the size of the specimens and the sexual maturity, aiming at comparisons with native and exotic populations, as well as interspecific and contributing to the understanding of its impact on the invaded ecosystems and the establishment of eradication programs. Thus, 39 females had evaluated the body biometry and the morphology and morphometry of the ovaries and oviducts. G2 (N=20): with Class I (>5-10mm) follicles, with Class I and Class II (>10-fold) follicles, 25mm) and G3 (N=9) with Class I, Class II and Class III (>25mm) follicles. Analysis of variance, Scott-Knott's test, and Pearson's correlation analysis showed that there was no significant difference between the groups in body biometry; in the mean gonadosomatic index and gonadal morphometry, only the width of the oviducts in the right antimer and the mass and width in the left antimer were higher in G3, the only one that presented eggs. There was positive and harmonic development between body mass, carapace, and plastron, and gonadal growth occurred concomitantly with body growth, indicating a higher reproductive potential and a positive relationship between the size of the litter and the female litter. The gonadosomatic index proved to be an excellent reproductive indicator, and the ovarian evaluation was a better indicator of sexual maturity than the maximum carapace length. Ovaries were irregular structures, without delimitation between the cortical and medullary regions and filled with vitelogenic follicles of different diameters, atresic follicles, and corpora lutea, which reflected the ovarian complexity of the species and the presence of follicular hierarchy. In the scarce stroma, two germinative beds were observed per ovary and the presence of gaps very close to the follicles and associated with the blood vessels. Analysis of gonadal tissue revealed three types of oocytes according to cytoplasmic characteristics: homogeneous, vesicular or vesicular in the cortex with apparent granules. Oviducts were functional and separated, joining only in the final portion to form the cloaca and subdivided into infundibulum, tuba, isthmus, uterus, and vagina. The structure of the uterine tube was composed of serosa, muscular and mucous, which was full of glands. The presence of eggs in the oviducts indicated that the specimens can reproduce in the Brazilian Cerrado. This study provides necessary and relevant information on the reproductive biology and capacity of T. scripta elegans in the Brazilian Cerrado and can contribute to the understanding of its impact on the invaded ecosystems and the establishment of eradication programs. The extraction of females with capacity can reduce the annual reproductive yield of the species and decrease its effect on local biodiversity.(AU)


Embora Trachemys scripta elegans seja uma espécie exótica popular como animal de estimação no Brasil, estudos sobre biologia e capacidade reprodutivas são inexistentes no Cerrado brasileiro. Este estudo analisou características ovarianas e do oviduto e a capacidade de produção de ovos em T. scripta elegans criadas neste bioma, correlacionando estes achados ao tamanho dos espécimes e a maturidade sexual, visando comparações com populações nativas e exóticas, bem como interespecíficas e contribuir para a compreensão de seu impacto nos ecossistemas invadidos e com o estabelecimento de programas de erradicação. Assim, 39 fêmeas tiveram avaliadas a biometria corporal e a morfologia e morfometria dos ovários e ovidutos. De acordo com o tamanho dos folículos ovarianos as fêmeas foram separadas em G1 (N= 10): com folículos Classe I (>5-10 mm), G2 (N= 20): com folículos Classe I e Classe II (>10-25 mm) e G3 (N= 9) com folículos Classe I, Classe II e Classe III (>25 mm). À análise de variância, teste de Scott-Knott e à análise de correlação de Pearson verificou-se que não houve diferença significativa entre os grupos na biometria corporal; no índice gonadossomático médio e na morfometria gonadal, apenas a largura dos ovidutos no antímero direito e a massa e a largura no antímero esquerdo foram maiores no G3, o único que apresentou ovos. Houve desenvolvimento positivo e harmônico entre massa corporal, carapaça e plastrão e o crescimento gonadal ocorreu concomitante ao crescimento corporal, indicando maior potencial reprodutivo e relação positiva entre o tamanho da ninhada de ovos e o da fêmea. O índice gonadossomático mostrou-se um bom indicador reprodutivo e a avaliação ovariana um melhor indicador da maturidade sexual que o comprimento máximo da carapaça. Ovários foram estruturas irregulares, sem delimitação entre a região cortical e medular e repletos de folículos vitelogênicos de diferentes diâmetros, folículos atrésicos e corpos lúteos, que refletiram a complexidade ovariana da espécie e a presença de hierarquia folicular. No estroma escasso foram observados dois leitos germinativos por ovário e a presença de lacunas muito próximas aos folículos e associadas aos vasos sanguíneos. A análise do tecido gonadal revelou três tipos de oócitos de acordo com as características do citoplasma: homogêneo, vesicular ou vesicular no córtex com grânulos aparentes. Ovidutos eram funcionais e separados, unindo-se apenas na porção final para formar a cloaca e subdividiam-se em infundíbulo, tuba uterina, istmo, útero e vagina. A estrutura da tuba uterina era constituída de serosa, muscular e mucosa, a qual era repleta de glândulas. A presença de ovos nos ovidutos indicou que os espécimes podem se reproduzir no cerrado brasileiro. Este estudo fornece informações básicas e relevantes da biologia e capacidade reprodutivas de T. scripta elegans no Cerrado brasileiro e pode contribuir com a compreensão de seu impacto nos ecossistemas invadidos e com o estabelecimento de programas de erradicação, uma vez que a extração de fêmeas com capacidade reprodutiva pode contribuir com a diminuição do rendimento reprodutivo anual da espécie e diminuir seu efeito sobre a biodiversidade local.(AU)


Subject(s)
Animals , Ovary/anatomy & histology , Oviducts/anatomy & histology , Turtles/anatomy & histology , Fallopian Tubes/anatomy & histology , Sexual Maturation , Corpus Luteum/anatomy & histology , Grassland , Ovarian Follicle/anatomy & histology
20.
Biol. Res ; 53: 24, 2020. tab, graf
Article in English | LILACS | ID: biblio-1124209

ABSTRACT

BACKGROUND: BMPR-1B is part of the transforming growth factor ß super family and plays a pivotal role in ewe litter size. Functional loss of exon-8 mutations in the BMPR-1B gene (namely the FecB gene) can increase both the ewe ovulation rate and litter size. RESULTS: This study constructed a eukaryotic expression system, prepared a monoclonal antibody, and characterized BMPR-1B/FecB protein-protein interactions (PPIs). Using Co-immunoprecipitation coupled to mass spectrometry (Co-IP/MS), 23 proteins were identified that specifically interact with FecB in ovary extracts of ewes. Bioinformatics analysis of selected PPIs demonstrated that FecB associated with several other BMPs, primarily via signal transduction in the ovary. FecB and its associated interaction proteins enriched the reproduction process via BMP2 and BMP4 pathways. Signal transduction was identified via Smads proteins and TGF-beta signaling pathway by analyzing the biological processes and pathways. Moreover, other target proteins (GDF5, GDF9, RhoD, and HSP 10) that interact with FecB and that are related to ovulation and litter size in ewes were identified. CONCLUSIONS: In summary, this research identified a novel pathway and insight to explore the PPi network of BMPR-1B.


Subject(s)
Animals , Female , Ovary/metabolism , Bone Morphogenetic Protein Receptors, Type I/genetics , Eukaryota/genetics , Protein Interaction Maps/genetics , Mass Spectrometry , Polymorphism, Restriction Fragment Length , Sheep , Signal Transduction , Polymerase Chain Reaction , Computational Biology , Bone Morphogenetic Protein Receptors, Type I/metabolism , Eukaryota/metabolism , Genotype , Mutation
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