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1.
Braz. dent. j ; 32(3): 21-31, May-June 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345507

ABSTRACT

Abstract This study assessed the fracture resistance of simulated immature teeth reinforced with calcium aluminate cement (CAC) or mineral trioxide aggregate (MTA) containing calcium carbonate nanoparticles (nano-CaCO3). The microstructural arrangement of the cements and their chemical constitution were also evaluated. Forty-eight canines simulating immature teeth were distributed into 6 groups (n=8): Negative control - no apical plug or root canal filling; CAC - apical plug with CAC; CAC/nano-CaCO3 - apical plug with CAC+5% nano-CaCO3; MTA - apical plug with MTA; MTA/nano-CaCO3 - apical plug with MTA+5% nano-CaCO3; and Positive control - root canal filling with MTA. The fracture resistance was evaluated in a universal testing machine. Samples of the cements were analyzed under Scanning Electron Microscope (SEM) to determine their microstructural arrangement. Chemical analysis of the cements was performed by Energy Dispersive X-ray Spectroscopy (EDS). The fracture resistance of CAC/nano-CaCO3 was significantly higher than the negative control (p<0.05). There was no significant difference among the other groups (p>0.05). Both cements had a more regular microstructure with the addition of nano-CaCO3. MTA samples had more calcium available in soluble forms than CAC. The addition of nano-CaCO3 to CAC increased the fracture resistance of teeth in comparison with the non-reinforced teeth. The microstructure of both cements containing nano-CaCO3 was similar, with a more homogeneous distribution of lamellar- and prismatic-shaped crystals. MTA had more calcium available in soluble forms than CAC.


Resumo Este estudo avaliou a resistência à fratura de dentes imaturos simulados reforçados com cimento de aluminato de cálcio (CAC) ou trióxido agregado mineral (MTA) contendo nanopartículas de carbonato de cálcio (nano-CaCO3). O arranjo microestrutural dos cimentos e sua constituição química também foram avaliados. Quarenta e oito caninos simulando dentes imaturos foram distribuídos em 6 grupos (n=8): Controle negativo - sem plug apical ou obturação do canal radicular; CAC - plug apical com CAC; CAC/nano-CaCO3 - plug apical com CAC + 5% nano-CaCO3; MTA - plug apical com MTA; MTA/nano-CaCO3 - plug apical com MTA + 5% nano-CaCO3; e Controle positivo - obturação dos canais radiculares com MTA. A resistência à fratura foi avaliada em máquina universal de ensaios. Amostras dos cimentos foram analisadas em Microscópio Eletrônico de Varredura (MEV) para determinar seu arranjo microestrutural. A análise química dos cimentos foi realizada por Espectroscopia de Energia Dispersiva de Raio-X (EDS). A resistência à fratura de CAC/nano-CaCO3 foi significativamente maior do que o controle negativo (p<0,05). Não houve diferença significativa entre os outros grupos (p>0,05). Ambos os cimentos apresentaram microestrutura mais regular com a adição de nano-CaCO3. As amostras de MTA apresentaram mais cálcio disponível em formas solúveis do que CAC. A adição de nano-CaCO3 ao CAC aumentou a resistência à fratura dos dentes em comparação aos dentes não reforçados. A microestrutura de ambos os cimentos contendo nano-CaCO3 foi semelhante, com uma distribuição mais homogênea de cristais de formato lamelar e prismático. MTA apresentou mais cálcio disponível nas formas solúveis do que CAC.


Subject(s)
Humans , Root Canal Filling Materials , Tooth Fractures , Oxides , Root Canal Obturation , Silicates , Calcium Compounds , Aluminum Compounds , Tooth Apex , Dental Cements , Drug Combinations
2.
Braz. dent. j ; 32(3): 65-74, May-June 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1345502

ABSTRACT

Abstract This study investigated the effect of three commercial calcium silicate-based materials (CSBM) on cytotoxicity and pro-and anti-inflammatory cytokines production in cultured human periodontal ligament stem cells (hPDLSCs). Culture of hPDLSCs was established and characterized. Extracts of Bio-C Sealer (Angelus, Londrina, PR, Brazil), MTA Fillapex (Angelus, Londrina, PR, Brazil) and PBS Cimmo HP (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brazil) were prepared by placing cement specimens (5 x 3 mm) in culture medium. Then, the extracts were serially two-fold diluted (1, 1:2, 1:4, 1:8, 1:16) and inserted into the cell-seeded wells for 24, 48 and 72 h for MTT assays. TNF-α and IL-10 cytokines were quantified by ELISA at 24h-cell supernatants. Data were analyzed by ANOVA and Tukey's test (α = 0.05). All CSBM exhibited some cytotoxicity that varied according to extract concentration and time of evaluation. MTA Fillapex presented the highest cytotoxic effects with significant reduction of metabolic activity/cell viability when compared to Bio-C Sealer and Cimmo HP®. TNF-α was significantly upregulated by the three tested cements (p < 0.05) while only MTA Fillapex significantly upregulated IL-10 in comparison to control. Taken collectively, the results showed that PBS Cimmo HP®, Bio-C Sealer and MTA Fillapex present mild and transient cytotoxicity and slightly induced TNF-α production. MTA Fillapex upregulated IL-10 release by hPDLSCs.


Resumo Este estudo investigou o efeito de três materiais comerciais à base de silicato de cálcio (CSBM) na citotoxicidade e na produção de citocinas pró e antiinflamatórias em células-tronco do ligamento periodontal humano (hPDLSCs). Cultura de hPDLSCs foi estabelecida e caracterizada. Extratos de Bio-C Sealer (Angelus, Londrina, PR, Brasil), MTA Fillapex (Angelus, Londrina, PR, Brasil) e PBS Cimmo HP® (Cimmo Soluções em Saúde, Pouso Alegre, MG, Brasil) foram preparados com a colocação de espécimes dos cimentos (5 x 3 mm) em meio de cultura. Em seguida, os extratos foram diluídos (1, 1: 2, 1: 4, 1: 8, 1:16) e inseridos nos poços semeados de células para ensaio de citotoxicidade por meio de MTT por 24, 48 e 72 h. As citocinas TNF-α e IL-10 foram quantificadas por ELISA em sobrenadantes de células de 24 h. Os dados foram analisados por ANOVA e teste de Tukey (α = 0,05). Todos os CSBM exibiram alguma citotoxicidade que variou de acordo com a concentração do extrato e o tempo de avaliação. O MTA Fillapex apresentou os maiores efeitos citotóxicos com redução significativa da atividade metabólica / viabilidade celular quando comparado ao Bio-C Sealer e Cimmo HP®. O TNF-α foi regulado positivamente pelos três cimentos testados (p <0,05), enquanto apenas o MTA Fillapex regulou positivamente a liberação de IL-10 em comparação com o controle. Tomados em conjunto, os resultados mostraram que PBS Cimmo HP®, Bio-C Sealer e MTA Fillapex apresentam citotoxicidade leve e transitória e induziram a produção de TNF-α. O MTA Fillapex regulou positivamente a liberação de IL-10 por hPDLSCs.


Subject(s)
Humans , Periodontal Ligament/cytology , Root Canal Filling Materials/adverse effects , Stem Cells/drug effects , Silicates/adverse effects , Calcium Compounds/adverse effects , Oxides , Materials Testing , Cytokines/metabolism , Aluminum Compounds
3.
Braz. dent. j ; 32(1): 53-58, Jan.-Feb. 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1180728

ABSTRACT

Abstract The endodontic revascularization may be an alternative treatment for necrotic immature teeth, however, several treatment steps may cause tooth discoloration. This study evaluated the use of three calcium silicate-based cements with different radiopacifying agents on the color alteration (∆E) of extracted premolars after simulation of revascularization. Forty single rooted extracted premolars were shaped with #1-6 gates Glidden drills, rinsed with sodium hypochlorite, and filled with fresh human blood. Three calcium silicate-based cements with different radiopacifying agents (bismuth oxide - CSBi, calcium tungstate - CSW, and zirconium oxide - CSZr) were applied over the blood clot (n=10). The control group received the application of a temporary zinc oxide-based cement (TFZn) (n=10). ∆E was measured with a spectrophotometer, using the L*a*b* color system of the International Commission on Illumination (CIELab), in different times: prior to the preparation of the access cavity (t0); right after treatment (t1); and after one (t2), two (t3), three (t4) and four (t5) months. The tooth site for color evaluation was standardized by silicon matrix, the color reading was performed 3 times per tooth, and the teeth were stored in 37º water between evaluations. ∆E, whiteness (WID index) and yellowness (b*) were evaluated. Data were subjected to one-way ANOVA and repeated measures ANOVA, followed by Tukey's post hoc test (α=0.05). All groups were similar in ∆E1 (t0-t1). The ∆E was the lowest and constant in the control group. In all evaluation times, CSBi presented the highest ∆E (p<0.01). CSW and CSZr were similar in all evaluated times and presented intermediate ∆E values. WID index from CSBi and CSW presented more distancing from 'white' reference. CSBi presented the greatest decrease in yellowness (b* value). The cement containing bismuth oxide presented the highest color alteration values. All tested calcium silicate-based cements presented clinically perceptible discoloration. Calcium tungstate and zirconium oxide may be used as alternative radiopacifiers to decrease tooth discoloration after endodontic tooth revascularization.


Resumo A revascularização endodôntica pode ser um tratamento alternativo para dentes imaturos necrosados, porém, várias etapas do tratamento podem causar alteração de cor dental. Este estudo avaliou o uso de três cimentos a base de silicato de cálcio com diferentes agentes radiopacificadores na alteração de cor (∆E) de pré-molares extraídos após a simulação de revascularização. Quarenta pré-molares unirradiculares extraídos foram conformados com brocas gates glidden #1-6, irrigados com hipoclorito de sódio, e preenchidos com sangue humano fresco. Três cimentos a base de silicato de cálcio com diferentes agentes radiopacificadores (óxido de bismuto - CSBi, tungstato de cálcio - CSW, e óxido de zircônio - CSZr) foram aplicados sobre o coágulo sanguíneo (n=10). O grupo controle recebeu a aplicação de um cimento temporário a base de óxido de zinco (TFZn) (n=10). ∆E foi medida com um espectrofotômetro, utilizando o sistema de cor L*a*b* da International Commission on Illumination (CIELab), em tempos diferentes: previamente ao preparo da cavidade de acesso (t0); logo após o tratamento (t1); e após um (t2), dois (t3), três (t4) e quatro (t5) meses. O local do dente para a avaliação de cor foi padronizado por uma matriz de silicone, a leitura da cor foi realizada 3 vezes por dente, e os dentes foram armazenados em água a 37ºC entre as avaliações. ∆E, 'clareamento' (índice WID) e tom amarelado (b*) foram avaliados. Os dados foram submetidos ao teste de ANOVA um fator e teste ANOVA de medidas repetidas, seguidos pelo teste post hoc de Tukey (α=0,05). Todos os grupos foram semelhantes em ∆E1 (t0-t1). A ∆E foi menor e constante no grupo controle. Em todos os tempos de avaliação, CSBi apresentou os maiores valores de ∆E (p<0.01). CSW e CSZr foram semelhantes em todos os tempos avaliados e apresentaram valores intermediários de ∆E. O índice WID de CSBi e CSW se distanciaram mais da referência 'branco'. CSBi apresentou maior diminuição no tom amarelado (valor de b*) os outros grupos. O cimento contendo óxido de bismuto apresentou os maiores valores de alteração de cor. Todos os cimentos testados apresentaram alteração de cor clinicamente perceptível. O tungstato de cálcio e óxido de zircônio podem ser usados como radiopacificares alternativos para a diminuição da alteração de cor dental após o processo de revascularização endodontica.


Subject(s)
Humans , Root Canal Filling Materials , Tooth Discoloration , Oxides , Silicates , Calcium Compounds , Aluminum Compounds , Dental Cements , Drug Combinations
4.
Braz. dent. j ; 32(1): 42-47, Jan.-Feb. 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1180727

ABSTRACT

Abstract New methodologies using micro-CT to evaluate solubility besides dimensional and morphological changes of endodontic materials are proposed. However, there is no standardization in the methods. The aim of this study was to assess the effect of different dimensions of test samples on volumetric change evaluation of different endodontic materials. AH Plus, FillCanal and Sealapex root canal sealers, Biodentine, IRM and MTA root-end filling cements were used in the tests. Samples of each material with a thickness of 1.5 mm and different diameters were manufactured: 6.3, 7.75, and 9.0 mm. The samples were scanned in micro-computed tomography (micro-CT) after setting and after 7 days of immersion in distilled water. The volumetric change was evaluated by means of the difference in the total volume of the specimens before and after immersion. Data were submitted to ANOVA and Tukey tests (p<0.05). The size of the samples did not affect the percentage of volumetric change of the materials (p>0.05). All sample sizes had greater volume loss for Sealapex among the sealers and Biodentine for the cements (p<0.05). In conclusion, Biodentine and Sealapex had the highest volume loss after immersion. Samples with 1.5 mm thickness, and diameters ranging between 6.3 and 9.0 mm can be used to assess the stability of endodontic materials using micro-CT without affecting the percentage of volumetric change.


Resumo Novas metodologias utilizando micro-CT são propostas para avaliar a solubilidade além de alterações dimensionais e morfológicas em materiais endodônticos. No entanto, não há padronização nos métodos. O objetivo deste estudo foi avaliar o efeito de diferentes dimensões de corpos de prova na avaliação da alteração volumétrica de diferentes materiais endodônticos. Os cimentos obturadores AH Plus, FillCanal e Sealapex e os cimentos retrobturadores Biodentine, IRM e MTA foram utilizados nos testes. Foram confeccionadas amostras de cada material com espessura de 1.5 mm e diâmetros diferentes: 6.3, 7.75 e 9.0 mm. As amostras foram escaneadas em microtomografia computadorizada (micro-CT) após a presa e após 7 dias de imersão em água destilada. A alteração volumétrica foi avaliada por meio da diferença no volume total dos corpos de prova antes e após a imersão. Os dados foram submetidos aos testes ANOVA e Tukey (p<0,05). A dimensão das amostras não afetou o percentual de alteração volumétrica dos materiais (p>0,05). Todos os diâmetros de amostra mostraram maior perda de volume para Sealapex entre os cimentos obturadores e Biodentine entre os cimentos retrobturadores (p<0,05). Como conclusão, Biodentine e Sealapex mostraram a maior perda volumétrica após a imersão. Amostras com 1.5 mm de espessura e diâmetros variando entre 6.3 e 9.0 mm podem ser usadas para avaliação da estabilidade de materiais endodônticos utilizando micro-CT, sem influenciar no percentual de alteração volumétrica.


Subject(s)
Root Canal Filling Materials , Oxides , Root Canal Obturation , Materials Testing , Calcium Compounds , Drug Combinations , Epoxy Resins , X-Ray Microtomography
5.
Chinese Journal of Biotechnology ; (12): 3745-3756, 2021.
Article in Chinese | WPRIM | ID: wpr-921462

ABSTRACT

Coronary artery disease (CAD) is a chronic disease but causes the highest mortality and morbidity among the cardiovascular diseases worldwide. Correlations between CAD and gut microbiota have been observed. This suggests that the gut microbiota could become a vital diagnostic marker of CAD, and restoring the gut habitat may become a promising strategy for CAD therapy. The elevated level of trimethylamine-N-oxide (TMAO), a gut microbiota-derived metabolite, was found to be associated with the increased risk of cardiovascular disease and the all-cause mortality. Preclinical studies have shown that it has pro-arteriosclerosis properties. It is likely that regulating the production of TMAO by gut microbiota may become a promising strategy for anti-atherosclerosis therapy. This review summarizes the clinical and preclinical researches on the intervention of CAD by regulating the gut microbiota and the microbiota-derived metabolite TMAO, with the aim to provide new target for the therapy of CAD.


Subject(s)
Coronary Artery Disease , Gastrointestinal Microbiome , Humans , Methylamines , Oxides
6.
Article in English | WPRIM | ID: wpr-921370

ABSTRACT

OBJECTIVES@#This study was performed to fabricate a bionic coating with titanium (Ti) phosphate to promote the osseointegration of Ti substrate implants.@*METHODS@#Phosphorylated micro/nanocoating was prepared on the surface of pure titanium (i.e., TiP-Ti) by hydrothermal process under special pressure, and the untreated smooth pure titanium (cp-Ti) was selected as the control. To evaluate the characteristics of the coating surface, scanning electron microscopy, X-ray diffraction, atomic force microscopy, and contact-angle measurement were performed. In addition, the effects of TiP-Ti on the proliferation, adhesion, and differentiation of rat bone marrow mesenchymal stem cells (BMSCs) were investigated by using @*RESULTS@#The TiP-Ti surface presented a bionic structure with coexisting nanoscale 3D spatial structure and microscale pores. @*CONCLUSIONS@#A bionic structure with TiP-Ti micro/nanoscale coating was successfully fabricated, indicating a promising method for modifying the surface of implants.


Subject(s)
Animals , Dental Implants , Osseointegration , Osteogenesis , Oxides , Phosphates , Rats , Surface Properties , Titanium
7.
Braz. oral res. (Online) ; 35: e086, 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1285724

ABSTRACT

Abstract This study evaluated the cytotoxicity, the antimicrobial and physicochemical properties of root canal sealers incorporated with phytotherapic Uncaria tomentosa (UT). Unmodified AH Plus (Dentsply, DeTrey, Germany) and MTA Fillapex (Angelus, Londrina, Brazil) were used as controls. UT was incorporated into AH Plus and MTA Fillapex, at concentrations of 2% and 5% of the total weight of these sealers (w/w). Flowability, setting time, and solubility were evaluated following ISO requirements. The pH values were measured at periods of 12, 24, 48 hours, and 7 days. The antimicrobial activity of the sealers against Enterococcus faecalis was analyzed by both direct contact tests in freshly prepared sealers, and after 7 days. The cytotoxicity of the samples was evaluated by the MTT assay, to check Balb/c 3T3 cell viability. The statistical analysis was performed by one-way ANOVA and Tukey's test (p < 0.05). The incorporation of UT was associated with a decrease in flow, for both sealers, an increase in AH Plus setting time, increase in MTA Fillapex pH values, and solubility (after 14 days), for both sealers (p < 0.05). Regarding the antibacterial evaluation, bacterial reduction was reported after incorporation of UT into both AH Plus and MTA Fillapex, up to 7 days after handling of the material (P<0.05). UT incorporation decreased the cytotoxic effects of both AH Plus and MTA Fillapex sealers in a way directly proportional to their respective concentrations (p < 0.05). In conclusion, UT can be added to both sealers to reduce their cytotoxicity, and improve their antibacterial effects, without compromising their original physicochemical properties.


Subject(s)
Humans , Root Canal Filling Materials/toxicity , Cat's Claw , Oxides , Materials Testing , Silicates , Calcium Compounds , Drug Combinations , Epoxy Resins/toxicity , Anti-Bacterial Agents/toxicity
8.
Braz. oral res. (Online) ; 35: e077, 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1278594

ABSTRACT

Abstract This study evaluated the physicochemical, biological, and antimicrobial properties of a new hydraulic calcium silicate-based modified material, and compared it with MTA Repair HP and MTA Angelus. The materials were assessed regarding color luminosity (L), color change, radiopacity, setting time, and ISO 6876:2012 linear flow. Volumetric filling and volume change were evaluated using microcomputed-tomography (µCT). Chemical characterization after 28 days in Hank's Balanced Salt Solution (HBSS) and pH analysis were also assessed. Biological characterization of cytotoxicity and microbiological assessment were also undertaken. Shapiro-Wilk, ANOVA, Levene and post hoc analyses with Bonferroni correction were performed, adopting a 5% significance level (p <0.05). Bio-C Pulpo exhibited the highest L values after 90 days. All tested materials demonstrated color change during the analyses, and had radiopacity above 5 mm Al. MTA Repair HP set faster than Bio-C Pulpo, whereas the latter had the highest linear flow. MTA Repair HP had the highest volumetric filling in µCT analysis. Bio-C Pulpo showed the highest alkalinity during all tested periods, and the highest volumetric loss (above 9%), in comparison with MTA Repair HP and MTA Angelus. Bio-C Pulpo did not form calcium hydroxide after hydration. MTA Repair HP demonstrated the highest cytocompatibility, and Bio-C Pulpo, the highest cytotoxicity. No inhibition halos were observed for any material, and similar higher turbidity values were seen after direct contact. Composition additives used in Bio-C Pulpo modified its properties, and both the absence of calcium hydroxide deposition after hydration, and the related cytotoxicity of this material are of particular concern.


Subject(s)
Root Canal Filling Materials/toxicity , Aluminum Compounds/toxicity , Oxides/toxicity , Materials Testing , Calcium , Silicates/toxicity , Calcium Compounds/toxicity , Drug Combinations
9.
Braz. dent. j ; 31(6): 680-684, Nov.-Dec. 2020. graf
Article in English | LILACS, BBO | ID: biblio-1132355

ABSTRACT

Abstract This case report discusses the endodontic treatment of a 7-year-old girl who suffered trauma (intrusion) to the immature upper central incisors secondary to a fall from a bicycle. Thirty days after the accident the patient was brought by her mother for clinical and radiographic assessment with a chief complaint of swelling and tenderness to percussion and palpation. Acute apical abscess associated with immature teeth were diagnosed. A decision was made to perform regenerative endodontic treatment. Access cavities were made and the root canals were disinfected by irrigation with 2.5% sodium hypochlorite. Final irrigation was performed with 17% EDTA. Due to pain and presence of secretions, 2% chlorhexidine gel was applied as an intracanal medicament. Seven days later, at the second visit, the root canals were once again disinfected and the canals of the right and left permanent upper central incisors were filled with double antibiotic paste (metronidazole/ciprofloxacin) and calcium hydroxide paste, respectively. Zinc oxide was mixed in both pastes. At the third visit, after 21 more days, the pastes were removed and the periapical areas were stimulated with a #80 K-file to encourage clot formation within the pulp cavities. A mineral trioxide aggregate (MTA) paste cervical plug was placed and the teeth were restored with glass ionomer cement. Radiographs and CBCT scans demonstrated complete root formations. The patient has been followed for 12 years, with evidence of clinical success throughout.


Resumo Este relato de caso discute o tratamento endodôntico de uma menina de 7 anos que sofreu trauma nos incisivos centrais superiores imaturos, após queda de bicicleta. Trinta dias após o acidente, a paciente foi levada pela mãe para avaliação clínica e radiográfica com queixa principal de inchaço e sensibilidade à percussão e palpação. Foram diagnosticados abscessos apicais agudos em ambos os dentes. Foi decidido por realizar o tratamento endodôntico regenerativo. Após acessos coronários, os canais radiculares foram submetidos à protocolos de antissepsia com auxílio de irrigação com hipoclorito de sódio a 2,5% e EDTA 17%. Devido à dor e presença de secreções, foi utilizada medicação intracanal com clorexidina gel 2% por 7 dias. Na segunda sessão, os canais radiculares foram novamente submetidos aos procedimentos de antissepsia e os canais dos incisivos centrais superiores permanentes direito e esquerdo foram preenchidos com pasta antibiótica dupla (Metronidazol / Ciprofloxacina) e pasta de hidróxido de cálcio, respectivamente. Em ambas as pastas foi adicionado o óxido de zinco. Na terceira sessão, após mais 21 dias, as pastas foram removidas e as regiões periapicais foram estimuladas com lima K nº 80 para incentivar a formação de coágulos sanguíneos no interior das cavidades pulpares. Tampões cervicais de MTA foram adaptados e os dentes restaurados com cimento de ionômero de vidro resinoso. Radiografias e tomografias computadorizadas demonstraram formações radiculares completas. A paciente é acompanhada há 12 anos, com evidências clínicas e radiográficas de sucesso.


Subject(s)
Humans , Female , Child , Regenerative Endodontics , Incisor , Oxides , Follow-Up Studies , Silicates , Calcium Compounds , Dental Pulp Necrosis , Abscess , Drug Combinations
10.
Braz. dent. j ; 31(5): 511-515, Sept.-Oct. 2020. tab
Article in English | LILACS, BBO | ID: biblio-1132334

ABSTRACT

Abstract This study aimed to assess the physicochemical properties of a repair material in the Brazilian market, BioMTA, in comparison to other two materials currently in use (Biodentine and MTA Angelus). The initial setting time was evaluated using Gillmore needle. The pH was measured with a pH-meter after 24 h, 3, 7, 14 and 21 days. The radiopacity was determined using the equivalence in millimeters of aluminum (mm Al) from digitized occlusal radiographs. Solubility was determined after immersion in water for 7 days. The data were analyzed by one-way ANOVA and Tukey tests (a=0.05). The BioMTA initial setting time (5.2 min) was lower than the other materials (p<0.05). All materials showed an alkaline pH at 21 days. At 24 h, BioMTA was the most alkaline material (p<0.05); and at 3, 7, 14 and 21 days there was no difference between BioMTA and Biodentine (p>0.05), both being more alkaline than MTA Angelus (p<0.05). The radiopacity of BioMTA (4.2 mm Al) was significantly higher compared to Biodentine (p<0.05) and lower than MTA Angelus (p<0.05). The solubility of the materials was -4.2%, -1.6% and 4.1% for BioMTA, MTA Angelus and Biodentine, respectively, with a significant difference between them (p<0.05). Therefore, it can be concluded that BioMTA displayed a shorter setting time, an alkaline pH, a higher radiopacity, and a gain in mass.


Resumo Este estudo teve como objetivo avaliar as propriedades físico-químicas de um material reparador disponível no mercado brasileiro, BioMTA, em comparação com outros dois materiais atualmente em uso (Biodentine e MTA Angelus). O tempo de presa inicial foi avaliado usando a agulha de Gillmore. O pH foi medido com um pH-metro após 24 h, 3, 7, 14 e 21 dias. A radiopacidade foi determinada aplicando a equivalência em milímetros de alumínio (mm Al) de radiografias oclusais digitalizadas. A solubilidade foi determinada após imersão em água por 7 dias. Os dados foram analisados ​​por meio dos testes ANOVA de uma via e Tukey (a=0,05). O tempo de presa inicial do BioMTA (5,2 min) foi menor do que os outros materiais (p<0,05). Todos os materiais apresentaram pH alcalino aos 21 dias. Às 24 h, o BioMTA foi o material mais alcalino (p<0,05); e aos 3, 7, 14 e 21 dias não houve diferença entre BioMTA e Biodentine (p>0,05), sendo ambos mais alcalinos que MTA Angelus (p<0,05). A radiopacidade do BioMTA (4,2 mm Al) foi significativamente maior em comparação ao Biodentine (p<0,05) e menor que o MTA Angelus (p<0,05). A solubilidade dos materiais foi de -4,2%, -1,6% e 4,1% para BioMTA, MTA Angelus e Biodentine, respectivamente, havendo diferença significativa entre eles (p<0,05). Portanto, pode-se concluir que o BioMTA apresentou um menor tempo de presa, pH alcalino, uma alta radiopacidade e um ganho em massa.


Subject(s)
Root Canal Filling Materials , Silicates , Oxides , Solubility , Acrylic Resins , Materials Testing , Brazil , Water , Calcium Compounds , Aluminum Compounds , Drug Combinations
11.
Braz. dent. j ; 31(5): 477-484, Sept.-Oct. 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132331

ABSTRACT

Abstract This study evaluated the biocompatibility, biomineralization, and collagen fiber maturation induced by Resorbable Tissue Replacement (RTR®; β-tricalcium phosphate [TCP]), Bioglass (BIOG; bioactive glass), and DM Bone® (DMB; hydroxyapatite and β-TCP) in vivo. Sixty-four polyethylene tubes with or without (control group; CG) materials (n=8/group/period) were randomly implanted in the subcutaneous tissue of 16 male Wistar rats (four per rat), weighting 250 to 280 g. The rats were killed after 7 and 30 days (n=8), and the specimens were removed for analysis of inflammation using hematoxylin-eosin; biomineralization assay using von Kossa (VK) staining and polarized light (PL); and collagen fiber maturation using picrosirius red (PSR). Nonparametric data were statistically analyzed by Kruskal-Wallis and Dunn tests, and parametric data by one-way ANOVA test (p<0.05). At 7 days, all groups induced moderate inflammation (p>0.05). At 30 days, there was mild inflammation in the BIOG and CG, and moderate inflammation in the RTR and DMB groups, with a significant difference between the CG and RTR (p<0.05). The fibrous capsule was thick at 7 days and predominantly thin at 30 days in all groups. All materials exhibited structures that stained positively for VK and PL. Immature collagen fibers were predominant at 7 and 30 days in all groups (p>0.05), although DMB exhibited more mature fibers than BIOG at 30 days (p<0.05). RTR, BIOG, and DMB were biocompatible, inducing inflammation that reduced over time and biomineralization in the subcutaneous tissue of rats. DMB exhibited more mature collagen fibers than BIOG over a longer period.


Resumo Este estudo avaliou a biocompatibilidade, biomineralização e maturação das fibras de colágeno induzidas por Resorbable Tissue Replacement (RTR®; fosfato β-tricálcico [TCP]), Bioglass (BIOG; vidro bioativo) e DM Bone® (DMB; hidroxiapatita e β-TCP) in vivo. Sessenta e quatro tubos de polietileno com ou sem (grupo controle; GC) os materiais (n=8/grupo/período) foram implantados aleatoriamente em tecido subcutâneo de 16 ratos machos Wistar (quatro por rato), pesando entre 250 a 280g. Os ratos foram mortos após 7 e 30 dias (n=8), e as amostras foram removidas para análise da inflamação utilizando hematoxilina-eosina; avaliação da biomineralização utilizando coloração de von Kossa (VK) e luz polarizada (LP); e maturação das fibras colágenas, utilizando picrosirius red (PSR). Os dados não-paramétricos foram analisados pelos testes de Kruskal-Wallis e Dunn, e os paramétricos pelo teste de one-way ANOVA (p<0.05). Aos 7 dias, todos os grupos induziram inflamação moderada (p>0,05). Aos 30 dias, houve inflamação leve nos grupos BIOG e GC, e inflamação moderada nos grupos RTR e DMB, com diferença significativa entre os GC e RTR (p<0,05). A cápsula fibrosa foi espessa aos 7 dias, e predominantemente fina aos 30 dias em todos os grupos. Todos os materiais exibiram estruturas positivas para VK e LP. Fibras colágenas imaturas foram predominantes aos 7 e 30 dias em todos os grupos (p>0,05), embora o DMB exibiu fibras mais maduras do que o BIOG aos 30 dias (p<0,05). RTR, BIOG e DMB foram biocompatíveis, induzindo inflamação que reduziu com o tempo, e biomineralização no tecido subcutâneo de ratos. O DMB exibiu mais fibras colágenas maduras do que o BIOG em período mais longo.


Subject(s)
Animals , Male , Rats , Root Canal Filling Materials , Biomineralization , Oxides , Biocompatible Materials , Materials Testing , Ceramics , Collagen , Rats, Wistar , Silicates , Calcium Compounds , Aluminum Compounds , Subcutaneous Tissue
12.
Int. j. odontostomatol. (Print) ; 14(2): 144-149, June 2020. graf
Article in English | LILACS | ID: biblio-1090666

ABSTRACT

Loss of teeth vitality when root formation is incomplete, results in weaker structures leaving them prone to fractures and unfavourable long-term prognosis. Apexogenesis is currently the treatment of choice in immature teeth and is indicated in vital teeth without pulpal pathologies. The treatment aims to eliminate the causal agent of the damage, and provide the necessary conditions to preserve vitality in the tooth and induce apical root closure. A 6-year-old male patient was treated at the Endodontics Clinic, Universidad de La Frontera upon complaining of acute pain in tooth 30. The tooth presented incomplete root development due to dental caries with pulp exposure and a diagnosis of irreversible symptomatic pulpitis. Total pulpotomy was performed with the application of Mineral Trioxide Aggregate and controlled at 1, 4, 6, 7 and 12 months, achieving root development and apical closure in the permanent molar. The result was comparable with studies that support this therapy in teeth with irreversible pulpitis. This work seeks to contribute to the existing evidence on the management of immature permanent teeth with irreversible pulpitis to induce root development and apical closure, and maintain pulp vitality.


La pérdida de vitalidad en dientes con formación radicular incompleta trae como resultado el debilitamiento de estos, dejándolos propensos a fracturas con un desfavorable pronóstico a largo plazo. Las terapéuticas actuales de regeneración pulpar en dientes inmaduros estan principalmente indicadas en cuadros de pulpitis irreversible y buscan eliminar el agente causal de daño y brindarle al diente las condiciones y estímulos necesarios para preservar vitalidad e inducir el cierre apical radicular. Un paciente de 6 años de edad y de sexo masculino, acude a la Clínica de Especialidad de Endodoncia de la Universidad de la Frontera, consultando por un dolor agudo en diente 4.6 el cual presentaba un desarrollo radicular incompleto producto de una caries con exposición pulpar con diagnóstico de Pulpitis Irreversible Sintomática. Se realiza una pulpotomia total con aplicación de Mineral Trioxide Aggregate y se controla a los 1, 4, 6 y 7 meses obteniendo un interesante resultado comparable con estudios que avalan dicha terapeutica en dientes con pulpitis irreversible. Este trabajo busca contribuir a la evidencia existente sobre el manejo de dientes permanentes inmaduros con cuadros de pulpitis irreversible para inducir el desarrollo radicular, cierre apical y mantener vitalidad pulpar.


Subject(s)
Humans , Male , Child , Oxides/administration & dosage , Pulpitis/therapy , Pulpotomy/methods , Silicates/administration & dosage , Calcium Compounds/administration & dosage , Aluminum Compounds/administration & dosage , Regeneration , Root Canal Filling Materials , Dentition, Permanent , Tooth, Nonvital/therapy , Dental Caries , Drug Combinations , Apexification
13.
Braz. dent. j ; 31(3): 252-256, May-June 2020. tab
Article in English | LILACS, BBO | ID: biblio-1132297

ABSTRACT

Abstract The purpose of this study was to investigate and to compare the physical-mechanical properties of a resin-modified calcium silicate material (TheraCal LC), used for pulp-capping, to MTA (Angelus) and a calcium hydroxide cement (Dycal). Specimens of each material (n=12) were prepared in Teflon molds (3.58 mm x 3 mm) and measured before and after immersion in distilled water for 24 h and 30 days to evaluate the dimensional change. The same specimens were submitted to compressive strength test on a Universal Testing Machine (Instron) (1 mm/min). Root canals were filled with the cements (n=8), and after 24 h, the bond strength (push-out test) to dentin was also assessed on a Universal Testing Machine (1 mm/min). Eight additional specimens of TheraCal LC were prepared to evaluate the bond strength immediately after light curing. Data were analyzed using One-Way ANOVA, and Tukey or Bonferroni post hoc tests (p<0.05). Percentage expansion of TheraCal LC was above the Specification No. 57 of ANSI/ADA, in both periods. The dimensional change for TheraCal LC was higher than MTA in 24 h and 30 days; and Dycal in 30 days (p<0.05). TheraCal LC had higher compressive and bond strength to dentin in comparison with MTA and Dycal (p<0.05). Although TheraCal LC expanded more than the ANSI/ADA recommendation, its compressive and push-out bond strength to dentin were satisfactory and superior to MTA and Dycal.


Resumo O objetivo deste estudo foi investigar e comparar as propriedades físico-mecânicas de um material à base de silicato de cálcio modificado por resina (TheraCal LC) ao MTA (Angelus) e um cimento de hidróxido de cálcio (Dycal). Espécimes de cada material (n=12) foram fabricados em moldes de Teflon (3,58 mm x 3 mm) e medidos antes e após imersão em água destilada por 24 h e 30 dias para avaliar a alteração dimensional. Os mesmos espécimes foram submetidos ao teste de resistência à compressão em uma Máquina Universal de Testes (Instron) (1 mm/min). Canais radiculares foram preenchidos com os cimentos (n=8), e após 24 h, a resistência de união (teste de push-out) também foi avaliada em uma Máquina Universal de Testes (1 mm/min). Oito amostras adicionais de TheraCal LC foram preparadas para avaliar a resistência de união imediatamente após a fotoativação. Os dados foram analisados utilizando os testes ANOVA de 1-fator e Tukey; ou Bonferroni (p<0,05). A expansão percentual do TheraCal LC ficou acima da Especificação No. 57 do ANSI/ADA, em ambos os períodos. Os valores de alteração dimensional para TheraCal LC foram maiores que MTA em 24 h e 30 dias; e Dycal em 30 dias (p<0,05). TheraCal LC apresentou maior resistência à compressão e união à dentina em comparação ao MTA e Dycal (p<0,05). Embora TheraCal LC tenha expandido mais que a recomendação da ANSI/ADA, sua resistência à compressão e de união à dentina foram satisfatórias e superiores ao MTA e Dycal.


Subject(s)
Dental Pulp Capping , Pulp Capping and Pulpectomy Agents , Oxides , Materials Testing , Silicates , Calcium Compounds , Aluminum Compounds , Drug Combinations
14.
Article in Chinese | WPRIM | ID: wpr-828047

ABSTRACT

The present study was aimed to investigate the effect of lime and licorice processing of Pinelliae Rhizoma on its toxic lectin protein and clarify the scientific detoxification connotation of lime and licorice processing of Pinelliae Rhizoma. Western blot was used to semi-quantitatively analyze the contents of lectin in Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum. Raw products and lectin were treated by soaking in licorice juice, lime solution or mixture solution of these two to investigate the different processing time on the content of toxic lectin protein. SDS-PAGE gel electrophoresis was used to analyze the changes of lectin protein bands in the solution and precipitates before and after processing. MALDI-TOF technology was used to qualitatively analyze and compare the protein molecular weight before and after processing. The results showed that the contents of lectin in Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum were 5.01% and 0.04% respectively, indicating that processing could significantly reduce the content of active lectin in raw products. The results also showed that the content of lectin in raw drugs decreased significantly after soaking in lime solution for one day or in licorice juice for three day, and the effect was greatest in mixture solution. Qualitative analysis showed that after being treated by soaking in lime solution, the lectin protein was decomposed into small peptide segments, while after being treated by soaking in licorice juice, the lectin protein was denatured and precipitated. The structure of lectin protein in Pinelliae Rhizoma was broken after being processed with licorice juice and lime solution, which significantly reduced the content of toxic lectinprotein. This is one of the detoxification mechanisms of Pinelliae Rhizoma processing.


Subject(s)
Calcium Compounds , Drugs, Chinese Herbal , Glycyrrhiza , Lectins , Oxides , Pinellia , Technology, Pharmaceutical
15.
Braz. oral res. (Online) ; 34: e102, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132708

ABSTRACT

Abstract The aim of this study was to evaluate and compare the clinical and radiographic success rates of pulpotomies in primary molars using a new stain-free tricalcium silicate cement (Bio-C Pulpo) versus a conventional white MTA (MTA Angelus) as a pulpotomy medicament for primary teeth. Seventy primary teeth in 33 patients were randomly divided into two groups, MTA Angelus (n = 34) and Bio-C Pulpo (n = 36). Clinical and radiographic follow-up examinations were conducted at seven days (except radiographic follow-up), and at one, three, six and 12 months. At 12 months, the clinical success rate of the MTA Angelus group was 100 percent (28 out of 28), and the radiographic success rate was 100 percent (28 out of 28). In comparison, the clinical success rate for the Bio-C Pulpo group was 100 percent (29 out of 29), and the radiographic success rate was 100 percent (29 out of 29). No significant differences were found between the groups at any follow-up evaluation. According to Fisher´s exact test, a significant difference was observed between the pulpotomy agent and tooth discoloration (p < 0.001). The success rates were 100% for the Bio-C Pulpo group, with no case of discoloration, and 10.31% for the MTA Angelus group. Both MTA Angelus and Bio-C Pulp pulpotomy medicaments are appropriate options for pulpotomies in primary teeth, and enable high clinical and radiographic success rates. However, more long-term studies are required to test the new Bio-C Pulpo medicament.


Subject(s)
Humans , Pulpotomy , Oxides , Tooth, Deciduous , Treatment Outcome , Silicates , Calcium Compounds , Aluminum Compounds , Dental Cements , Drug Combinations
16.
Article in English | LILACS | ID: biblio-1283651

ABSTRACT

This study presents analyses on the chemistry, biology, pharmacology and chromatography of essential oils extracted from three species of the Ocotea genus: O. minor, O. ceanothifolia and O. leucoxylon. Leaves and stems, as well as the bark of O. minor, were processed using a modified Clevenger apparatus. Seven essential oils were obtained and analyzed using GC-FID and GC-MS, and their chemical compositions were determined. Assays of cytotoxicity, antioxidant and free radical scavenging activity, as well as tyrosinase and elastase inhibition were performed. In total, 25 constituents were identified, the principal being sesquiterpenes, such as spathulenol caryophyllene and its oxide. The oils did not present cytotoxicity using a hemolytic model, but also did not show antioxidant activity in the DPPH assay. Essential oil from stems of O. ceanothifolia, rich in spathulenol and caryophyllene oxide, demonstrated the capacity to inhibit 49.08% of tyrosinase activity at a concentration of 100 µg/mL. This research contributes to the chemical profile analysis of the three species of Ocoteathrough chemical investigations and biological activity, which are reported for the first time here in this study.


Este trabajo realiza un estudio químico, biológico, farmacológico y cromatográfico de aceites esenciales extraídos de tres especies del género Ocotea: O. minor, O. ceanothifoliay O. leucoxylon. Las hojas y tallos, así como la corteza de O. minor, se procesaron utilizando un aparato Clevenger modificado. Se obtuvieron siete aceites esenciales y se analizaron usando GC-FID y GC-MS, y se determinaron sus composiciones químicas. Se realizaron ensayos de citotoxicidad, actividad antioxidante y de atrapamiento de radicales libres, así como inhibición de tirosinasa y elastasa. En total, se identificaron 25 componentes, siendo los principales sesquiterpenos, como el spathulenol cariofileno y su óxido. Los aceites no presentaron citotoxicidad en un modelo hemolítico y tampoco mostraron actividad antioxidante en el ensayo con DPPH. El aceite esencial de tallos de O. ceanothifolia, rico en espatulenol y óxido de cariofileno, mostró capacidad para inhibir el 49.08% de la actividad de tirosinasa a una concentración de 100 µg/mL. Esta investigación contribuye al análisis del perfil químico de las tres especies de Ocotea a través de investigaciones químicas y actividad biológica la cual se informan por primera vez.


Subject(s)
Oils, Volatile/pharmacology , Monophenol Monooxygenase/antagonists & inhibitors , Ocotea/chemistry , Oxides/analysis , Sesquiterpenes/analysis , Oils, Volatile/chemistry , Free Radical Scavengers , Lauraceae/chemistry , Cosmetic Industry , Flame Ionization , Gas Chromatography-Mass Spectrometry
17.
São Paulo; s.n; 2020. 98 p.
Thesis in Portuguese | LILACS | ID: biblio-1147548

ABSTRACT

Introdução - A carne é um elemento central na alimentação, entretanto, diferentes condições podem levar a formação de produtos da oxidação do colesterol (POCs). Também os efeitos do excesso de sódio na pressão arterial estão bem documentados. Assim, o uso de sal de ervas poderia reduzir o teor de sódio e a formação de POCs. Objetivo - Avaliar os efeitos de diferentes concentrações de sal de ervas na aceitação da redução de sódio e na proteção contra a oxidação lipídica em hambúrguer. Material e Métodos - Os hambúrgueres foram adicionados de dois níveis de sal (0,60% e 1,90%) e dois níveis de orégano, alecrim e manjericão (0,36% e 0,72%). Aos controles FC1 (0,60% de sal) e FC2 (1,90% de sal), foram adicionadas duas concentrações de ervas, compondo as formulações F3 (0,60% de sal e 0,36% de ervas), F4 (1,90% de sal e 0,36% de ervas), F5 (0,60% de sal e 0,72% de ervas) e F6 (1,90% de sal e 0,72% de ervas). A aceitação sensorial foi realizada por escala hedônica e de intensidade do sabor. A cor e textura foram avaliadas por análises instrumentais. Foi realizada a caracterização físico-química das amostras cruas e grelhadas. A avaliação da oxidação lipídica foi realizada logo após o preparo e após 30, 60 e 90 dias de congelamento. Foram determinados os compostos fenólicos totais e a capacidade antioxidante das ervas. Resultados - Todas as formulações foram aceitas, sendo FC2, F3 e F4 as mais aceitas. No atributo sabor, F3 obteve média maior que FC2, indicando maior aceitação do sabor após adição de sal de ervas, em comparação a adição isolada de sal no teor regularmente consumido. A cor obteve médias de aceitação maiores para os controles. A análise instrumental da dureza não apresentou variações, porém pela avaliação sensorial as ervas auxiliaram na maciez. A maioria das formulações foram consideradas com salinidade e condimentação ideal. A retenção de umidade após a cocção foi maior com o uso de ervas. Os teores de fenólicos totais no orégano foi de 16,46 ± 0,25 mg EAG/g, no alecrim de 17,80 ± 0,18 mg EAG/g e no manjericão 3,24 ± 0,34 mg EAG/g. O uso de ervas não inibiu a oxidação lipídica durante a cocção e após, o armazenamento, a inibição ocorreu no teor reduzido de sal (F3 e F5 com 21% e 16%, respectivamente, em 30 dias e 54% e 59%, respectivamente, em 60 dias) e, em 90 dias, apenas F3 apresentou inibição de 22%. Os teores de colesterol variaram entre 35,25 ± 5,34 a 87,13 ± 8,05 mg/100g de amostra seca e a concentração de POCs totais foi de 68,19 ± 2,89 a 123,68 ± 4,51 µg/g de amostra seca. As formulações com ervas não apresentaram menores teores de POCs que os controles. Conclusão - Todas as formulações foram aceitas em todos os atributos, com maior aceitação a formulação adicionada de teor de sal reduzido e do menor teor de ervas, sendo também a formulação com menor oxidação e redução de sal de 40%. A adição de ervas não apresentou efeito sinérgico contra a formação de POCs.


Introduction - Meat is central element in the diet; however, different conditions can lead to the formation of cholesterol oxidation products (COPs). The effects of excess sodium on blood pressure are also well documented. Thus, the use of herbal salt could reduce the sodium content and the formation of POCs. Objective - To evaluate the effects of different concentrations of herbal salt on the acceptance of sodium reduction and protection against lipid oxidation in hamburgers. Material and Methods - The hamburgers were formulated with two levels of sodium chloride (0.60% and 1.90%) and two levels of herbal mixture (0.36% and 0.72%), composed of oregano, rosemary and basil. To the controls FC1 (0.60% of salt) and FC2 (1.90% of salt), different herbs proportions were added: F3 (0.60% of salt and 0.36% of the herbal mix), F4 (1.90% of the salt and 0.36% of the herbal mix), F5 (0.60% of the salt and 0.72% of the herbal mix) and F6 (1.90% of the salt and 0.72% herbal mix). Sensory acceptance was achieved by hedonic scale and flavor intensity. The color and texture were evaluated by instrumental analyzes. The physical and chemical characterization of raw and grilled samples was carried out. The evaluation of lipid oxidation was performed immediately after preparation and after 30, 60 and 90 days of freezing. The total phenolic compounds and the antioxidant capacity of the herbs were determined. Results - All formulations were accepted, with FC2, F3 and F4 being the most accepted. In the flavor attribute, F3 obtained an average greater than FC2, demonstrating greater acceptance of flavor after adding herbal salt, compared to salt in the content regularly consumed. The color obtains higher acceptance averages for the controls. An instrumental analysis of the hardness does not show variations, however, in the sensorial evaluation the herbs helped in the softness. Most formulations were evaluated with ideal salinity and seasoning. Moisture retention after cooking was greater with the use of herbs. The content of total phenolics in oregano was 16.46 ± 0.25 mg EAG / g, in rosemary 17.80 ± 0.18 mg EAG / g and in basil 3.24 ± 0.34 mg EAG / g. The use of herbs did not inhibit lipid oxidation during cooking and after storage the inhibition occurred reduced salt content (F3 and F5 with 21% and 16%, respectively, in 30 days and 54% and 59%, respectively, in 60 days) and in 60 days only F3 showed 22% inhibition. Cholesterol levels vary between 35.25 ± 5.34 to 87.13 ± 8.05 mg / 100g of dry sample and the concentration of total COPs was 68.19 ± 2.89 to 123.68 ± 4.51 µg / g of dry sample. The herbal formulations has no lower COPs content than controls. Conclusions - All formulations were accepted in all attributes, with preference for the formulation added with reduced salt content and the lowest herbal content, and also the formulation with less oxidation and 40% salt reduction. The addition of herbs has no synergistic effect against the formation of POCs.


Subject(s)
Oxides , Sodium , Cholesterol , Condiments , Meat
18.
J. appl. oral sci ; 28: e20190023, 2020. graf
Article in English | LILACS | ID: biblio-1056577

ABSTRACT

Abstract When exposure of the pulp to external environment occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain pulp tissue vitality and function. These clinical situations require the use of materials that induce dentin repair and, subsequently, formation of a mineralized tissue. Objective: This work aims to assess the effect of tricalcium silicate cements and mineral trioxide aggregate cements, including repairing dentin formation and inflammatory reactions over time after pulp exposure in Wistar rats. Methodology: These two biomaterials were compared with positive control groups (open cavity with pulp tissue exposure) and negative control groups (no intervention). The evaluations were performed in three stages; three, seven and twenty-one days, and consisted of an imaging (nuclear medicine) and histological evaluation (H&E staining, immunohistochemistry and Alizarin Red S). Results: The therapeutic effect of these biomaterials was confirmed. Nuclear medicine evaluation demonstrated that the uptake of 99mTc-Hydroxymethylene diphosphonate (HMDP) showed no significant differences between the different experimental groups and the control, revealing the non-occurrence of differences in the phosphocalcium metabolism. The histological study demonstrated that in mineral trioxide aggregate therapies, the presence of moderate inflammatory infiltration was found after three days, decreasing during follow-ups. The formation of mineralized tissue was only verified at 21 days of follow-up. The tricalcium silicate therapies demonstrated the presence of a slight inflammatory infiltration on the third day, increasing throughout the follow-up. The formation of mineralized tissue was observed in the seventh follow-up day, increasing over time. Conclusions: The mineral trioxide aggregate (WhiteProRoot®MTA) and tricalcium silicate (Biodentine™) present slight and reversible inflammatory signs in the pulp tissue, with the formation of mineralized tissue. However, the exacerbated induction of mineralized tissue formation with the tricalcium silicate biomaterial may lead to the formation of pulp calcifications


Subject(s)
Animals , Male , Oxides/pharmacology , Biocompatible Materials/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Dental Pulp/drug effects , Dentin/drug effects , Dentinogenesis/drug effects , Phosphoproteins/analysis , Pulpitis/pathology , Pulpitis/drug therapy , Sialoglycoproteins/analysis , Time Factors , Immunohistochemistry , Random Allocation , Reproducibility of Results , Extracellular Matrix Proteins/analysis , Dental Pulp Exposure/pathology , Dental Pulp Exposure/drug therapy , Rats, Wistar , Dental Pulp/pathology , Dental Pulp Capping/methods , Drug Combinations , Molecular Imaging/methods , Pulp Capping and Pulpectomy Agents/pharmacology , Odontoblasts/drug effects
19.
J. appl. oral sci ; 28: e20190105, 2020. tab, graf
Article in English | LILACS | ID: biblio-1056578

ABSTRACT

Abstract Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.


Subject(s)
Humans , Mice , Calcium Compounds/pharmacology , Calcium Compounds/chemistry , Aluminum Compounds/pharmacology , Aluminum Compounds/chemistry , Dental Cements/pharmacology , Dental Cements/chemistry , Dental Pulp/cytology , Dental Pulp/drug effects , Oxides/pharmacology , Oxides/chemistry , Time Factors , Zinc Oxide/pharmacology , Zinc Oxide/chemistry , Bismuth/pharmacology , Bismuth/chemistry , Materials Testing , Calcium Chloride/pharmacology , Calcium Chloride/chemistry , Gene Expression/drug effects , Cell Survival/drug effects , Cells, Cultured , Reproducibility of Results , Silicates/pharmacology , Silicates/chemistry , Drug Combinations , Alkaline Phosphatase/analysis , Alkaline Phosphatase/drug effects , Odontoblasts/drug effects
20.
J. appl. oral sci ; 28: e20200033, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1134805

ABSTRACT

Abstract Aim To evaluate the cytotoxicity, biocompatibility and mineralization capacity of BIO-C PULPO, and MTA. Methodology L929 fibroblasts were cultured and MTT assay was used to determine the material cytotoxicity on 6, 24, and 48 h. A total of 30 male rats (Wistar) aged between 4 and 6 months, weighing between 250 and 300 g were used. Polyethylene tubes containing BIO-C PULPO, MTA, and empty tubes were implanted into dorsal connective tissue. After the experimental periods (7, 15, 30, 60, and 90 days) the tubes were histologically analyzed using hematoxylin-eosin (H&E), immunolabeling of IL-1β and TNF-α, and von Kossa staining, or without staining for polarized light analysis. The average number of inflammatory cells was quantified; the mineralization assessment was determined by the area marked in μm2 and semiquantitative immunolabeling analyses of IL-1β and TNF-α were performed. Then, data underwent statistical analysis with a 5% significance level. Results It was observed that BIO-C PULPO and MTA presented cytocompatibility at 6, 24, and 48 similar or higher than control for all evaluated period. On periods 7 and 15 days, BIO-C PULPO was the material with the highest number of inflammatory cells (p<0.05). On periods 30, 60, and 90 days, BIO-C PULPO and MTA presented similar inflammatory reactions (p>0.05). No statistical differences were found between Control, BIO-C PULPO, and MTA for immunolabeling of IL-1β and TNF-α in the different periods of analysis (p<0.05). Positive von Kossa staining and birefringent structures under polarized light were observed in all analyzed periods in contact with both materials, but larger mineralization area was found with BIO-C PULPO on day 90 (p<0.05). Conclusion BIO-C PULPO was biocompatible and induced mineralization similar to MTA.


Subject(s)
Animals , Male , Rats , Root Canal Filling Materials , Tumor Necrosis Factor-alpha/metabolism , Dental Cements , Interleukin-1beta/metabolism , Biomineralization , Oxides , Biocompatible Materials , Rats, Wistar , Silicates , Calcium Compounds , Aluminum Compounds , Subcutaneous Tissue , Drug Combinations , Inflammation
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