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1.
Electron. j. biotechnol ; 52: 35-44, July. 2021. tab, ilus
Article in English | LILACS | ID: biblio-1283494

ABSTRACT

BACKGROUND: Alginates are polysaccharides used in a wide range of industrial applications, with their functional properties depending on their molecular weight. In this study, alginate production and the expression of genes involved in polymerization and depolymerization in batch cultures of Azotobacter vinelandii were evaluated under controlled and noncontrolled oxygen transfer rate (OTR) conditions. RESULTS: Using an oxygen transfer rate (OTR) control system, a constant OTR (20.3 ± 1.3 mmol L 1 h 1 ) was maintained during cell growth and stationary phases. In cultures subjected to a controlled OTR, alginate concentrations were higher (5.5 ± 0.2 g L 1 ) than in cultures under noncontrolled OTR. The molecular weight of alginate decreased from 475 to 325 kDa at the beginning of the growth phase and remained constant until the end of the cultivation period. The expression level of alyA1, which encodes an alginate lyase, was more affected by OTR control than those of other genes involved in alginate biosynthesis. The decrease in alginate molecular weight can be explained by a higher relative expression level of alyA1 under the controlled OTR condition. CONCLUSIONS: This report describes the first time that alginate production and alginate lyase (alyA1) expression levels have been evaluated in A. vinelandii cultures subjected to a controlled OTR. The results show that automatic control of OTR may be a suitable strategy for improving alginate production while maintaining a constant molecular weight.


Subject(s)
Polysaccharide-Lyases/metabolism , Oxygen Transfer , Azotobacter vinelandii/metabolism , Oxygen/metabolism , Gene Expression , Polymerase Chain Reaction , Azotobacter vinelandii/genetics , Alginates/metabolism , Fermentation , Molecular Weight
2.
Rev. Pesqui. Fisioter ; 11(2): 435-444, Maio 2021. ilus, tab
Article in English, Portuguese | LILACS | ID: biblio-1254018

ABSTRACT

INTRODUÇÃO: O pré-condicionamento isquêmico remoto (PCIR) é uma intervenção cardioprotetora não invasiva que atenua a lesão celular sofrida por uma isquemia prolongada. Seus efeitos de proteção sobre o coração, quando aplicado ao esporte, pode melhorar o desempenho do exercício. OBJETIVO: Investigar o efeito do pré-condicionamento isquêmico remoto no consumo máximo de oxigênio (VO2máx) e potência máxima (Wmáx) em corredores e ciclistas. METODOLOGIA: Revisão sistemática e metanálise, com ensaios clínicos randomizados. Baseado no PRISMA e avaliado pelo repositório de projetos de revisões sistemática PROSPERO; entretanto, não obteve o registro por se tratar de um desfecho de performance esportiva. As buscas foram realizadas nas bases de dados Medline/PubMed, SciELO, Periódicos CAPES. A seleção dos estudos foi realizada em duas etapas: leitura do título e resumo, e leitura completa dos artigos. A extração dos dados foi realizada pela transcrição das informações. A qualidade metodológica foi avaliada pela escala risco de viés através da ferramenta Cochrane. Excluíram-se estudos que investigaram variáveis diferentes dos desfechos selecionados para esta revisão. RESULTADOS: Foram incluídos oito ensaios clínicos. Verificou-se que nos itens geração de sequência aleatória, ocultação de alocação e cegamento de avaliadores de desfecho em quase todos os estudos tiveram alto risco de viés. Os resultados da metanálise revelou VO2máx (p < 0,01), o PCIR mostrou ser eficaz; Wmáx não houve diferença significativa. CONCLUSÃO: O pré-condicionamento isquêmico remoto pode ser capaz de aumentar o VO2máx em corredores e ciclistas. A Wmáx demonstra não ser influenciada pelo PCIR.


INTRODUCTION: Remote ischemic preconditioning (PIRC) is a non-invasive cardioprotective intervention that attenuates cell damage suffered by prolonged ischemia. Its protective effects on the heart, when applied to sport, can improve exercise performance. OBJECTIVE: To investigate the effect of remote ischemic preconditioning on maximum oxygen consumption (VO2max) and maximum power (Wmax) in runners and cyclists. METHODOLOGY: Systematic review and metaanalysis, with randomized clinical trials. Based on PRISMA and evaluated by the PROSPERO systematic review project repository; however, it did not obtain registration because it is an outcome of sports performance. The searches were carried out in the Medline / PubMed, SciELO, Capes Periodicals databases. The selection of studies was carried out in two stages: reading the title and summary and reading the articles in full. Data extraction was performed by transcribing the information. Methodological quality was assessed by the risk of bias scale using the Cochrane tool. Studies that investigated variables other than the outcomes selected for this review were excluded. RESULTS: Eight clinical trials were included. In the generation of the item of random sequence, concealment of allocation and blinding of outcome evaluators in almost all studies had a high risk of bias. The analysis of the risk of bias was high risk. The meta-analysis results revealed VO2max (p <0.01), the PCIR proved to be effective; Wmax there was no significant difference. CONCLUSION: Remote ischemic preconditioning may be able to increase VO2max in runners and cyclists. Wmax demonstrates that the PCIR does not influence it.


Subject(s)
Cardiorespiratory Fitness , Oxygen , Exercise
3.
Goiânia; SES-GO; 12 mar. 2021. 1-5 p. fig.
Non-conventional in Portuguese | LILACS, ColecionaSUS, CONASS, SES-GO | ID: biblio-1247400

ABSTRACT

A pandemia do novo coronavírus trouxe grande sobrecarga aos sistemas de saúde de todo o mundo, especialmente aos países de baixa e média renda (BONG CL et al, 2020). O atraso da vacinação tem agravado a situação nestes países, aumentando o índice de novos casos e a mortalidade pela doença (WHO, 2021). Um dos problemas enfrentados tem sido a escassez da oferta de oxigênio (O2) hospitalar. Agências internacionais como Wellcome Trust, Unitaid e MS criaram uma força tarefa em busca de uma resposta emergencial para a situação, estimando a necessidade de um aporte financeiro de cerca de 90 milhões de dólares, a fim de se evitar mortes preveníveis pela falta de oxigênio para o manejo clínico dos pacientes hospitalizados por COVID-19 (USHER AD, 2021).


The pandemic of the new coronavirus has brought great burden to health systems around the world, especially to low- and middle-income countries (BONG CL et al, 2020). The delay of vaccination has aggravated the situation in these countries, increasing the rate of new cases and mortality from the disease (WHO, 2021). One of the problems faced has been the scarcity of hospital oxygen (O2) supply. International agencies such as Wellcome Trust, Unitaid and MS have set up a task force in search of an emergency response to the situation, estimating the need for a financial contribution of about US$90 million in order to avoid preventable deaths from the lack of oxygen for the clinical management of patients hospitalized by COVID-19 (USHER AD, 2021).


Subject(s)
Humans , Male , Female , Pregnancy , Infant, Newborn , Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Oxygen/physiology , Oxygen/supply & distribution , Oxygen Consumption/drug effects
4.
Braz. dent. j ; 32(1): 91-97, Jan.-Feb. 2021. tab, graf
Article in English | LILACS, BBO | ID: biblio-1180719

ABSTRACT

Abstract This study investigated the effects of oxygen inhibition and finishing/polishing procedures on the composite resin properties. One bulk-fill and two conventional composite resins (nanoparticle and microhybrid) were evaluated. Specimens were prepared using 4 surface treatments: control, no treatment; Gly, oxygen inhibition with glycerin; FP, finishing and polishing; Gly + FP, glycerin followed by finishing and polishing. The degree of conversion (DC) was measured using Fourier Transformed Infrared Spectroscopy (FTIR) immediately and after 15 days (n=5). Color stability (ΔEab, and ΔE00) and opacity were evaluated using a spectrophotometer after 15 days of immersion in coffee, using the CIELAB system (n=5). Data were analyzed by two-way ANOVA and Tukey tests (α=0.05) and opacity by two-way repeated-measures ANOVA. Glycerin usage increased significantly the DC however had no influence on the ΔEab, ΔE00 and, opacity values. Finishing and polishing reduced ΔEab and ΔE00 values, regardless of composite resins. Microhybrid showed higher opacity, followed by the nanoparticle and bulk fill, regardless of surface treatment. Post-polymerization polishing procedures resulted in lower conversion than using an oxygen inhibitor agent (Gly condition), but similar staining caused by coffee.


Resumo Este estudo investigou os efeitos da inibição de oxigênio e dos procedimentos de acabamento/polimento nas propriedades das resinas compostas. Foram avaliadas uma resina composta bulk fill e duas resinas convencionais (nanoparticulada e microhíbrida). Os espécimes foram confeccionados, variando o tratamento de superfície: controle, sem tratamento; glicerina (inibidor de oxigênio); acabamento e polimento; glicerina + acabamento e polimento. O grau de conversão (GC) foi medido pela Espectroscopia de Infravermelho Transformada de Fourier (FTIR) imediatamente e após 15 dias da confecção dos espécimes (n=5). Os índices de alteração de cor ΔEab, ΔE00 e opacidade foram avaliados por meio de espectrofotômetro, após 15 dias de imersão no café, utilizando o sistema CIELAB (n=5). Os dados da alteração da cor foram analisados pelos testes ANOVA de dois fatores e Tukey (α=0,05) e a opacidade por ANOVA de medidas repetidas. O uso de glicerina aumentou significativamente o GC, no entanto, não teve influência sobre os valores ΔEab e ΔE00. O acabamento e o polimento reduziram os valores de ΔEab e ΔE00, independentemente da resina composta. A resina microhíbrida apresentou maior opacidade, seguida pela nanoparticula e bulk-fill, independentemente do tratamento de superfície. O acabamento e polimento resultou em menor grau de conversão das resinas compostas se comparado ao uso de um agente inibidor de oxigênio (glicerina), porém apresenta resultados similares para a pigmentação pelo café.


Subject(s)
Oxygen , Composite Resins , Surface Properties , Materials Testing , Color , Dental Materials , Dental Polishing
6.
Washington; Organización Panamericana de la Salud; feb. 26, 2021. 32 p.
Non-conventional in Spanish | LILACS | ID: biblio-1151146

ABSTRACT

Este documento tiene como objetivo dar las facilitar recomendaciones para asegurar la capacidad de suministro de oxígeno para oxigenoterapia en los módulos asistenciales de los equipos médicos de emergencia (EMT) y en los sitios alternativos de atención médica (SAAM). El documento incluye conocimientos básicos sobre los diferentes tipos de instalaciones de oxigenoterapia, así como las orientaciones para que el personal de apoyo operacional del EMT pueda realizar una adaptación óptima de sus equipos para atender las necesidades clínicas de los pacientes COVID-19.


Subject(s)
Oxygen/supply & distribution , Oxygen Inhalation Therapy/methods , Pneumonia, Viral/prevention & control , Coronavirus Infections/prevention & control , Hospital Rapid Response Team/organization & administration , Pandemics/prevention & control
7.
Article in Chinese | WPRIM | ID: wpr-880467

ABSTRACT

In order to obtain comprehensive brain activity information conveniently in real time, this study designs a portable EEG and blood oxygen synchronous acquisition system for real-time monitoring of brain functional activities. The EEG electrodes filter and amplify the detected EEG signals, and send them to the microprocessor via Bluetooth to analyze the EEG data; the photoelectric probe converts the optical signals into electrical signals, which are amplified and separated, filtered, and AD converted, calculates the brain's oxygenation and blood-red protein (ΔHbO


Subject(s)
Brain , Electrodes , Electroencephalography , Oxygen
9.
Article in English | WPRIM | ID: wpr-879965

ABSTRACT

Microalgae is an easy-to-obtain natural biological material with many varieties and abundant natural reserves. Microalgae are rich in natural fluorescein, which can be used as a contrast agent for fluorescence imaging and photoacoustic imaging for medical imaging. With its active surface, microalgae can effectively adsorb functional molecules, metal elements, etc., and have good application prospects in the field of drug delivery. Microalgae can generate oxygen through photosynthesis to increase local oxygen concentration, reverse local hypoxia to enhance the efficacy of hypoxic tumors and promote wound healing. In addition, microalgae have good biocompatibility, and different administration methods have no obvious toxicity. This paper reviews the research progress on the biomedical application of microalgae in bioimaging, drug delivery, hypoxic tumor treatment, wound healing.


Subject(s)
Drug Delivery Systems , Humans , Hypoxia , Microalgae , Oxygen , Wound Healing
10.
Article in English | WPRIM | ID: wpr-879951

ABSTRACT

:To investigate the effect of transient receptor potential melastatin 2 (TRPM2) inhibitor A10 on oxygen glucose deprivation/reperfusion (OGD/R) injury in SH-SY5Y cells.:Human neuroblastoma SH-SY5Y cells were subject to OGD/R injury,and then were divided into blank control group,model control group and A10 group randomly. The cell survival rate was detected by cell counting kit 8 (CCK-8); the level of cellular reactive oxygen species (ROS) was detected by reactive oxygen detection kit; the mitochondrial membrane potential was detected by tetramethylrhodamine (TMRM) method; the number of apoptotic cells was detected by TUNEL apoptosis assay kit; the protein expression level of cleaved caspase 3 was detected by Western blot.:Compared with 3,20,30,50, has lower cytotoxicity and better inhibition effect on channel activity. Compared with the model control group,ROS level was reduced,the mitochondrial membrane potential was improved,the number of apoptosis cells was reduced ,and the expression of cleaved caspase 3 was significantly reduced in the A10 group(all <0.05). : A10 can alleviate cell damage after OGD/R by inhibiting TRPM2 channel function,reducing extracellular calcium influx,reducing cell ROS levels,stabilizing mitochondrial membrane potential levels,and reducing apoptosis.


Subject(s)
Apoptosis , Benzeneacetamides , Cell Survival , Glucose , Humans , Oxygen/metabolism , Piperidones , Reactive Oxygen Species/metabolism , Reperfusion , TRPM Cation Channels
11.
Acta Physiologica Sinica ; (6): 286-294, 2021.
Article in Chinese | WPRIM | ID: wpr-878257

ABSTRACT

Many studies have shown that high-altitude exposure could significantly influence human cognition, and the approaches which could enhance the human cognition in high-altitude hypoxia environment attract great attention. In the present study, we recruited a total of 60 subjects who had been migrated to Tibet University as adults for more than one year. These participants were randomly divided into the experimental group and the control group. The participants in the experimental group were instructed to complete a hyperbaric oxygen treatment, and those in the control group just completed a wait condition. By using the attention network test (ANT), the changes of the attention function before and after a single session of hyperbaric oxygen treatment were explored. The results showed that single hyperbaric oxygen treatment significantly improved the orienting function of attention, with an obvious post-intervention effect, but not the alerting and conflict function of attention. We also found a strong association between alerting function and conflict function after the end of intervention, suggesting the change of the overall performance of attention function. The present findings might suggest that the improvement of attention function by a single session of hyperbaric oxygen intervention is derived from the increase of general cognitive resources, rather than the transfer of cognitive resources within the attention system.


Subject(s)
Adult , Humans , Hyperbaric Oxygenation , Orientation , Oxygen , Reaction Time , Tibet , Transients and Migrants
12.
Chinese Medical Journal ; (24): 699-707, 2021.
Article in English | WPRIM | ID: wpr-878065

ABSTRACT

BACKGROUND@#Autophagy of alveolar macrophages is a crucial process in ischemia/reperfusion injury-induced acute lung injury (ALI). Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent cells with the potential for repairing injured sites and regulating autophagy. This study was to investigate the influence of BM-MSCs on autophagy of macrophages in the oxygen-glucose deprivation/restoration (OGD/R) microenvironment and to explore the potential mechanism.@*METHODS@#We established a co-culture system of macrophages (RAW264.7) with BM-MSCs under OGD/R conditions in vitro. RAW264.7 cells were transfected with recombinant adenovirus (Ad-mCherry-GFP-LC3B) and autophagic status of RAW264.7 cells was observed under a fluorescence microscope. Autophagy-related proteins light chain 3 (LC3)-I, LC3-II, and p62 in RAW264.7 cells were detected by Western blotting. We used microarray expression analysis to identify the differently expressed genes between OGD/R treated macrophages and macrophages co-culture with BM-MSCs. We investigated the gene heme oxygenase-1 (HO-1), which is downstream of the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathway.@*RESULTS@#The ratio of LC3-II/LC3-I of OGD/R treated RAW264.7 cells was increased (1.27 ± 0.20 vs. 0.44 ± 0.08, t = 6.67, P  < 0.05), while the expression of p62 was decreased (0.77 ± 0.04 vs. 0.95 ± 0.10, t = 2.90, P  < 0.05), and PI3K (0.40 ± 0.06 vs. 0.63 ± 0.10, t = 3.42, P  < 0.05) and p-Akt/Akt ratio was also decreased (0.39 ± 0.02 vs. 0.58 ± 0.03, t = 9.13, P  < 0.05). BM-MSCs reduced the LC3-II/LC3-I ratio of OGD/R treated RAW264.7 cells (0.68 ± 0.14 vs. 1.27 ± 0.20, t = 4.12, P  < 0.05), up-regulated p62 expression (1.10 ± 0.20 vs. 0.77 ± 0.04, t = 2.80, P  < 0.05), and up-regulated PI3K (0.54 ± 0.05 vs. 0.40 ± 0.06, t = 3.11, P  < 0.05) and p-Akt/Akt ratios (0.52 ± 0.05 vs. 0.39 ± 0.02, t = 9.13, P  < 0.05). A whole-genome microarray assay screened the differentially expressed gene HO-1, which is downstream of the PI3K/Akt signaling pathway, and the alteration of HO-1 mRNA and protein expression was consistent with the data on PI3K/Akt pathway.@*CONCLUSIONS@#Our results suggest the existence of the PI3K/Akt/HO-1 signaling pathway in RAW264.7 cells under OGD/R circumstances in vitro, revealing the mechanism underlying BM-MSC-mediated regulation of autophagy and enriching the understanding of potential therapeutic targets for the treatment of ALI.


Subject(s)
Apoptosis , Autophagy , Bone Marrow , Glucose , Heme Oxygenase-1/metabolism , Macrophages/metabolism , Mesenchymal Stem Cells/metabolism , Oxygen , Phosphatidylinositol 3-Kinase , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , Signal Transduction
13.
Einstein (Säo Paulo) ; 19: eAO6069, 2021. tab, graf
Article in English | LILACS | ID: biblio-1286308

ABSTRACT

ABSTRACT Objective To evaluate the ability of blood-oxygen-level-dependent (BOLD) magnetic resonance imaging at 3 Tesla to measure tissue oxygen bioavailability based on R2* values, and to differentiate between acute tubular necrosis and acute rejection compared to renal biopsy (gold standard). Methods A prospective, single-center study, with patients submitted to renal transplantation between 2013 and 2014, who developed graft dysfunction less than 4 weeks after transplantation. All patients were submitted to abdominal magnetic resonance imaging at 3 Tesla using the same protocol, followed by two BOLD sequences and kidney biopsy. Results Twelve male (68.75%) and three female (31.25%) patients were included. A total of 19 percutaneous renal biopsies were performed (four patients required a second biopsy due to changes in clinical findings). Pathological findings revealed ten cases of acute tubular necrosis, four cases of acute rejection, and five cases with other (miscellaneous) diagnoses. Comparison between the four groups of interest failed to reveal significant differences (p=0.177) in cortical R2* values, whereas medullary R2* values differed significantly (p=0.033), with lower values in the miscellaneous diagnoses and the acute tubular necrosis group. Conclusion BOLD magnetic resonance imaging at 3 Tesla is a feasible technique that uses indirect tissue oxygen level measurements to differentiate between acute rejection and acute tubular necrosis in renal grafts.


RESUMO Objetivo Avaliar a sequência de ressonância magnética blood-oxygen-level-dependent (BOLD) realizada em um equipamento 3 Tesla para medir a biodisponibilidade do oxigênio do tecido pelo valor de R2* na diferenciação de necrose tubular aguda e rejeição aguda, comparando à biópsia renal (padrão-ouro). Métodos Estudo unicêntrico, prospectivo, com pacientes submetidos a transplante renal de 2013 a 2014, que desenvolveram disfunção do enxerto menos de 4 semanas após o transplante. Todos os pacientes foram submetidos à ressonância magnética abdominal 3 Tesla com o mesmo protocolo, seguida de duas sequências BOLD e biópsia renal. Resultados Foram incluídos 12 homens (68,75%) e três mulheres (31,25%). Foram realizadas 19 biópsias renais percutâneas (quatro pacientes necessitaram de segunda biópsia devido à alteração nos achados clínicos). Os resultados histopatológicos incluíram dez casos de necrose tubular aguda, quatro de rejeição aguda e cinco casos de outros diagnósticos (miscelânea). A comparação entre os quatro grupos de interesse não mostrou diferenças significativas em relação ao R2* no córtex (p=0,177). Quanto ao R2* da medula, observaram-se diferenças significativas (p=0,033), com miscelânea e necrose tubular aguda apresentando valores mais baixos quando comparados aos demais. Conclusão A ressonância magnética BOLD 3 Tesla é uma técnica viável que indiretamente utiliza a concentração de oxigênio no tecido em enxertos renais e pode ser usada para a diferenciação entre rejeição aguda e necrose tubular aguda.


Subject(s)
Humans , Male , Female , Kidney Transplantation , Oxygen , Biopsy , Prospective Studies , Allografts
14.
Acta toxicol. argent ; 28(3): 1-10, dic. 2020. graf
Article in Spanish | LILACS | ID: biblio-1284970

ABSTRACT

Resumen Se realiza una revisión narrativa que plantea una reflexión acerca del rol de la oxigenación hiperbárica en la recuperación de los intoxicados con monóxido de carbono (ICO). La relación presión de tratamiento de oxigenación hiperbárica (TOHB), o sea dosis de oxígeno, y demora en su implementación son descriptas en esta revisión. Se presentan 9 casos de pacientes con ICO tratados con TOHB a 1,45 ATA (Atmósferas absolutas) por falta de acceso a TOHB de alta presión. Si bien es necesario investigación adicional, sugerimos que esta modalidad terapéutica a 1,45 ATA para ICO debe ser elegida frente al oxígeno normobárico, y considerada cuando las instalaciones de alta presión no están disponibles a distancias razonables.


Abstract A narrative review that raises a reflection about the role of hyperbaric oxygenation in the recovery of monoxide carbon (CO) poisoning is carried out. A description of the relationship of the pressure of hyperbaric oxygen therapy (HBOT), oxygen dosage, and the delay in its implementation was done. Nine cases of intoxications treated with HBOT at 1.45 ATA due to lack of access to high-pressure HBOT were presented. While additional research is necessary, we suggest that this therapeutic modality at 1.45 ATA (Absolute Atmospheres) should be chosen instead of normobaric oxygen therapy for CO poisoning, and considered when high pressure facilities are not available at reasonable distances.


Subject(s)
Humans , Male , Female , Child , Adult , Middle Aged , Aged , Oxygen/administration & dosage , Carbon Monoxide Poisoning/therapy , Hyperbaric Oxygenation , Oxygen/therapeutic use , Argentina/epidemiology , Carbon Monoxide Poisoning/complications , Carbon Monoxide Poisoning/epidemiology , Retrospective Studies
15.
Rev. Assoc. Med. Bras. (1992) ; 66(11): 1577-1582, Nov. 2020. tab, graf
Article in English | LILACS, SES-SP | ID: biblio-1143642

ABSTRACT

SUMMARY Hepatopulmonary Syndrome (HPS) is a complication of cirrhosis that worsens the disease's prognosis, pre and post liver transplant. The objective of this study is to analyze the prevalence of HPS in cirrhotic patients at our service and to correlate it with oxygen saturation (SatO2) using a pulse oximeter to evaluate if this is useful as a screening test for HPS. A prospective study was conducted in patients with hepatic cirrhosis conventionally selected from 2014 to 2016. All the patients underwent an echocardiogram with microbubbles and oxygen saturation measurement by pulse oximetry. Those with intrapulmonary shunt were submitted to arterial blood gas analysis. The relationship between oxygen saturation and HPS was assessed by the multivariate model of binary logistic regression. We analyzed 77 patients, and 23.3% (18 patients) had all criteria for HPS. The relationship between HPS and SatO2 did not show statistical significance, even after the variables were adjusted for sex, age, and smoking. Oxygen saturation alone was not able to detect HPS in the sample of cirrhotic patients. More accurate methods for screening and diagnosis of the syndrome should be used.


RESUMO A Síndrome Hepatopulmonar (SHP) é uma complicação da cirrose que piora o prognóstico da doença pré e pós-transplante hepático. O objetivo do trabalho é analisar a prevalência de SHP em pacientes cirróticos de nosso serviço e correlacioná-la com a saturação de oxigênio (SatO2) pelo oxímetro de pulso, e avaliar se este seria útil como um exame de triagem no diagnóstico de SHP. Foi realizado um estudo prospectivo em pacientes portadores de cirrose hepática no período de 2014 a 2016. Todos os pacientes foram submetidos a um ecocardiogama com microbolhas e a saturação de oxigênio pela oximetria de pulso. Aqueles com shunt intrapulmonar foram submetidos a gasometria arterial. A relação entre a saturação de oxigênio e SHP foi avaliada pelo modelo multivariado de regressão logística binário. Foram analisados 77 pacientes, destes 23,3% (18 pacientes) apresentaram todos os critérios para SHP. A relação entre a SHP com a SatO2 não obteve significância estatística, mesmo após as variáveis terem sido ajustadas pelo sexo, idade e tabagismo atual ou passado. A saturação de oxigênio, de forma isolada, não foi capaz de distinguir a SHP na amostra de pacientes cirróticos em nosso estudo. Deve-se utilizar métodos mais acurados para a triagem e diagnóstico dessa síndrome.


Subject(s)
Humans , Hepatopulmonary Syndrome/diagnosis , Hepatopulmonary Syndrome/etiology , Oxygen , Oximetry , Prospective Studies , Liver Cirrhosis/complications
16.
Arq. bras. cardiol ; 115(5): 821-827, nov. 2020. tab
Article in Portuguese | LILACS, SES-SP | ID: biblio-1142272

ABSTRACT

Resumo Fundamento O tratamento com sacubitril-valsartana teve seu benefício prognóstico confirmado no ensaio PARADIGM-HF. No entanto, dados sobre alterações no teste de esforço cardiopulmonar (TECP) com o uso de sacubitril-valsartana são escassos. Objetivo O objetivo deste estudo foi comparar os parâmetros do TECP antes e depois do tratamento com sacubitril-valsartana. Métodos Avaliação prospectiva de pacientes com insuficiência cardíaca (IC) crônica e fração de ejeção do ventrículo esquerdo ≤40%, mesmo sob terapia padrão otimizada, que iniciaram tratamento com sacubitril-valsartana, sem expectativa de tratamentos adicionais para a IC. Os dados do TECP foram coletados na semana anterior e 6 meses depois do tratamento com sacubitril-valsartana. Diferenças estatísticas com valor p <0,05 foram consideradas significativas. Resultados De 42 pacientes, 35 (83,3%) completaram o seguimento de 6 meses, uma vez que 2 (4,8%) morreram e 5 (11,9%) interromperam o tratamento devido a eventos adversos. A média de idade foi de 58,6±11,1 anos. A classe NYHA (classificação da New York Heart Association) melhorou em 26 (74,3%) pacientes. O consumo máximo de oxigênio (VO2max) (14,4 vs. 18,3 ml/kg/min, p<0,001), a inclinação VE/VCO2 (36,7 vs. 31,1, p<0,001) e a duração do exercício (487,8 vs. 640,3 s, p<0,001) também melhoraram com o uso de sacubitril-valsartana. O benefício foi mantido mesmo com a dose de 24/26 mg (13,5 vs. 19,2 ml/kg/min, p=0,018) de sacubitril-valsartana, desde que esta tenha sido a maior dose tolerada. Conclusões O tratamento com sacubitril-valsartana está associado a uma melhora acentuada do VO2max, da inclinação VE/VCO2 e da duração do exercício no TECP. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0)


Abstract Background Sacubitril/valsartan had its prognosis benefit confirmed in the PARADIGM-HF trial. However, data on cardiopulmonary exercise testing (CPET) changes with sacubitril-valsartan therapy are scarce. Objective This study aimed to compare CPET parameters before and after sacubitril-valsartan therapy. Methods Prospective evaluation of chronic heart failure (HF) patients with left ventricular ejection fraction ≤40% despite optimized standard of care therapy, who started sacubitril-valsartan therapy, expecting no additional HF treatment. CPET data were gathered in the week before and 6 months after sacubitril-valsartan therapy. Statistical differences with a p-value <0.05 were considered significant. Results Out of 42 patients, 35 (83.3%) completed the 6-month follow-up, since 2 (4.8%) patients died and 5 (11.9%) discontinued treatment for adverse events. Mean age was 58.6±11.1 years. New York Heart Association class improved in 26 (74.3%) patients. Maximal oxygen uptake (VO2max) (14.4 vs. 18.3 ml/kg/min, p<0.001), VE/VCO2slope (36.7 vs. 31.1, p<0.001), and exercise duration (487.8 vs. 640.3 sec, p<0.001) also improved with sacubitril-valsartan. Benefit was maintained even with the 24/26 mg dose (13.5 vs. 19.2 ml/kg/min, p=0.018) of sacubitril-valsartan, as long as this was the highest tolerated dose. Conclusions Sacubitril-valsartan therapy is associated with marked CPET improvement in VO2max, VE/VCO2slope, and exercise duration. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0)


Subject(s)
Humans , Middle Aged , Aged , Ventricular Function, Left , Heart Failure/drug therapy , Oxygen , Stroke Volume , Tetrazoles , Prospective Studies , Treatment Outcome , Drug Combinations , Angiotensin Receptor Antagonists , Aminobutyrates
18.
Lima; Perú. Ministerio de Salud; 20201100. 39 p. ilus.
Monography in Spanish | LILACS, MINSAPERU | ID: biblio-1130139

ABSTRACT

El documento contiene las disposiciones y criterios técnicos para el uso de oxígeno medicinal en las Instituciones Prestadoras de Servicios de Salud, públicas, privadas y mixtas del sector salud. Deroga a la R.M N° 879-2020


Subject(s)
Oxygen , Oxygen Inhalation Therapy , Guidelines as Topic , Health Facilities , Health Services
19.
Arq. ciências saúde UNIPAR ; 24(3): 203-208, set-dez. 2020.
Article in Portuguese | LILACS | ID: biblio-1129431

ABSTRACT

A Ozonioterapia, método que utiliza a mistura gasosa de ozônio e oxigênio, é uma das Práticas Integrativas aprovadas pelo Ministério da Saúde. Atualmente é utilizada na Odontologia devido, principalmente, às suas características estruturais que permitem vastas aplicações. Essa Prática tem como principal objetivo os fins terapêuticos, por meio de propostas cada vez mais efetivas, que corroboram para técnicas e métodos capazes de propiciar tratamentos complementares a fim de curar e prevenir patologias inerentes à cavidade oral, contribuindo para a potencialização de resultados das técnicas já existentes. Em virtude da busca por técnicas complementares, a Ozonioterapia ganhou destaque e tem se mostrado efetiva e segura em diversas práticas odontológicas. O presente trabalho tem por objetivo, por meio de uma revisão de literatura, ressaltar as aplicabilidades do Ozônio (O3) na área odontológica, explicitando meios de utilização e suas respectivas ações, bem como, as contraindicações frente às ocorrências que acometem a cavidade oral.


Ozone therapy, a method that uses a gaseous mixture of ozone and oxygen, is one of the Integrative Practices approved by the Brazilian Ministry of Health. It is currently used in dentistry mainly due to its structural characteristics that allow a wide range of applications. This Practice focuses on therapeutic purposes through increasingly effective proposals that corroborate techniques and methods capable of providing complementary treatments in order to cure and prevent pathologies inherent to the oral cavity, contributing to the potentiation of results from existing techniques. Due to the search for complementary techniques, ozone therapy has gained prominence and has proven to be both effective and safe in several dental practices. This work aims at highlighting the applicability of Ozone (O3) in the dental area through a literature review, explaining means of use and their respective actions, as well as the contraindications to occurrences that affect the oral cavity.


Subject(s)
Ozone/analysis , Therapeutics , Dentistry , Oxygen/analysis , Bacteria , Oral Surgical Procedures , Dentists , Endodontics
20.
Neumol. pediátr. (En línea) ; 15(3): 402-405, sept. 2020. tab, ilus
Article in Spanish | LILACS | ID: biblio-1127612

ABSTRACT

A clinical case of Neuroendocrine Cell Hyperplasia is presented with a bibliographic review. An infant patient with respiratory distress syndrome, characterized by nasal flaring, retractions, and tachypnea with temporary resolution with the use of bronchodilators. However, the patient requires oxygen. With complementary examinations (negative viral panel twice) and epidemiology it is classified as a viral Bronchiolitis. Without improvement, extrapulmonar pathologies were suspected, discarding hearth disease, epilepsy, pathological gastroesophageal reflux. New tests were performed to rule out other pathologies, including immunological disorders. Those results were normal, so a high-resolution chest tomography was done which allowed the diagnosis of Neuroendocrine Cell Hyperplasia. During the follow up the child had improved and required oxygen until he was two years old. Neuroendocrine Cell Hyperplasia belongs to a huge group of less common interstitial disorders, which diagnosis is clinical and radiological. It can easily be confused with common respiratory disorders. For this reason, it is important to know about this disease to make an early diagnosis. Most of the cases had a gradual (months to years) improvement.


Se presenta un caso clínico de Hiperplasia de Células Neuroendocrinas y la revisión de la literatura. Paciente lactante menor con cuadro de dificultad respiratoria, caracterizado por aleteo nasal, retracciones y taquipnea persistente acompañada de desaturación. Sin adecuada respuesta al uso de broncodilatadores. Por exámenes complementarios, panel viral negativo en dos ocasiones y epidemiología, se le diagnostica una bronquiolitis viral. Por no presentar mejoría se completan estudios, descartándose neumonía atípica, cardiopatía, epilepsia, reflujo gastroesofágico patológico y compromiso inmunológico. El diagnóstico fue determinado en base a la clínica, junto con imágenes en vidrio esmerilado característicos en lóbulo medio y língula. En su seguimiento mejora paulatinamente, requiriendo soporte de oxígeno hasta los dos años. La Hiperplasia de Células Neuroendocrinas es una patología intersticial pulmonar poco frecuente, cuyo diagnóstico es clínico y radiológico. Puede ser fácilmente confundida con desórdenes respiratorios comunes, por lo que es importante sospecharla para realizar un diagnóstico precoz. La mayor parte de los casos evolucionan con declinación de los síntomas, mejorando espontáneamente en meses o en los primeros años de vida.


Subject(s)
Humans , Infant , Lung Diseases, Interstitial/diagnosis , Neuroendocrine Cells/pathology , Hyperplasia/diagnosis , Oxygen/therapeutic use , Lung Diseases, Interstitial/therapy , Tachypnea/etiology , Hyperplasia/therapy
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