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Article in English | WPRIM | ID: wpr-188651


Eosinophil degranulation plays a crucial role in tissue inflammatory reactions associated with helminth parasitic nfections and allergic diseases. Paragonimus westermani, a lung fluke causing human paragonimiasis, secretes a large amount of cysteine proteases, which are involved in nutrient uptake, tissue invasion, and modulation of hos's immune responses. There is, however, limited information about the response of eosinophils to direct stimulation by cysteine proteases (CP) secreted by P. westermani. In the present study, we tested whether degranulation and superoxide production from human eosinophils can be induced by stimulation of the 2 CP (27 kDa and 28 kDa) purified from excretory-secretory products (ESP) of P. westermani newly excysted metacercariae (PwNEM). A large quantity of eosinophil-derived neurotoxin (EDN) was detected in the culture supernatant when human eosinophils isolated from the peripheral blood were incubated with the purified 27 kDa CP. Furthermore, the 27 kDa CP induced superoxide anion production by eosinophils in time- and dose-dependent manners. In contrast, the purified 28 kDa CP did not induce superoxide production and degranulation. These findings suggest that the 27 kDa CP secreted by PwNEM induces superoxide production and degranulation of human eosinophils, which may be involved in eosinophil-mediated tissue inflammatory responses during the larval migration in human paragonimiasis.

Animals , Astacoidea/parasitology , Cell Degranulation , Cysteine Endopeptidases/immunology , Eosinophils/immunology , Helminth Proteins/immunology , Humans , Paragonimiasis/immunology , Paragonimus westermani/enzymology , Superoxides/immunology
Article in English | IMSEAR | ID: sea-31889


The time course of humoral immune response to Paragonimus siamensis was studied in 10 cats experimentally infected with either 30 or 60 matacercariae and the antibody produced was detected by enzyme linked immunosorbent assay (ELISA), complement fixation test (CFT), and immunoelectrophoresis (IEP) test. With ELISA and CFT, antibodies was detected as early as 2nd week after infection and the cats remained positive throughout the 12 week period of observation. In contrast, the IEP test was persistently negative. With respect to sensitivity, both ELISA and CFT are equally sensitive but the mean ELISA titer was consistently higher than that of CFT. THe magnitude of the antibody response appeared to be related to duration of the infection but not to the infective dose and the number of worms recovered. There was variability in titers among cats infected with equal numbers of metacercariae. The tests can not be used for differential diagnosis of infections by P. siamensis and P. heterotremus because of the cross-reaction. Such cross-reaction did not not occur against unrelated parasites including hookworm. Toxocara cati and Spirometra mansoni.

Animals , Antibody Formation , Cat Diseases/immunology , Cats , Complement Fixation Tests , Enzyme-Linked Immunosorbent Assay , Female , Immunoelectrophoresis , Male , Paragonimiasis/immunology