Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 28
Filter
1.
Int. j. morphol ; 38(6): 1639-1644, Dec. 2020. graf
Article in English | LILACS | ID: biblio-1134491

ABSTRACT

SUMMARY: Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavernosum of rats submitted to a semivoluntary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression of AIF and PARP and their regulatory microRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoluntary alcoholism.


RESUMEN: Estudios previos de nuestro grupo describieron las consecuencias del uso de etanol en la erección del pene. Sin embargo, los mecanismos moleculares que rodean a los microARN, el proceso de apoptosis y su relación con la disfunción eréctil asociada con el consumo de alcohol aún no se conocen bien. El objetivo de este análisis fue evaluar el mecanismo de apoptosis mediante la expresión de AIF y PARP, así como sus microARN reguladores: miR-145, miR-210 y miR-486, en el cuerpo cavernoso de ratas sometidas a un modelo de alcoholismo semivoluntario. Se dividieron 24 ratas Wistar en dos grupos: control (C) grupo de ratas tratadas con etanol al 20 % (A) durante siete semanas. Las muestras del cuerpo cavernoso se prepararon para el análisis inmunohistoquímico de la expresión de la proteína AIF y PARP, y la expresión del gen microRNAs miR-145, miR-210, miR-486 en tejido cavernoso se realizó por PCR en tiempo real. El análisis inmunohistoquímico mostró escaso etiquetado nuclear positivo para la proteína PARP y AIF en el cuerpo cavernoso de los animales de control y tratados con etanol. Después del análisis de la expresión de microARN miR-145, -210 y -486 no se encontraron resultados con diferencias estadísticas significativas entre los grupos control y alcoholizados. La expresión de AIF y PARP y sus microARN reguladores involucrados en el proceso apoptótico (miR-145, miR-210 y miR-486) no se alteraron en el cuerpo cavernoso de las ratas sometidas a alcoholismo semivoluntario.


Subject(s)
Animals , Rats , Apoptosis , Alcoholism/metabolism , Erectile Dysfunction/metabolism , Penis/physiopathology , Penis/chemistry , Immunohistochemistry , Rats, Wistar , MicroRNAs/analysis , MicroRNAs/genetics , MicroRNAs/metabolism , Disease Models, Animal , Alcoholism/physiopathology , Apoptosis Inducing Factor/analysis , Apoptosis Inducing Factor/genetics , Apoptosis Inducing Factor/metabolism , Real-Time Polymerase Chain Reaction , Erectile Dysfunction/physiopathology
2.
Int. braz. j. urol ; 45(5): 1033-1042, Sept.-Dec. 2019. tab, graf
Article in English | LILACS | ID: biblio-1040068

ABSTRACT

ABSTRACT Cinnamomum cassia (Cinnamon) is a well-known traditional medicine with therapeutic benefits for centuries. We evaluated the effects of cinnamon essential oil (CEO) and its main component cinnamaldehyde (CA) on human corpus cavernosum (HCC) and rat CC. The essential oil of cinnamon was analyzed for the confirmation of the oil profile. HCC specimens from patients undergoing penile prosthesis surgery (age 48-69 years) were utilized for functional studies. In addition, erectile responses in anesthetized control and diabetic rats were evaluated in vivo after intracavernosal injection of CEO and CA, and rat CC strips were placed in organ baths. After precontraction with phenylephrine (10µM), relaxant responses to CEO and CA were investigated. CA (96.9%) was found as the major component. The maximum relaxation responses to CEO and CA were 96.4±3.5% and 96.0±5.0% in HCC and 97.5±5.5% and 96.8±4.8% in rat CC, respectively. There was no difference between control and diabetic rats in relaxation responses to CEO and CA. The relaxant responses obtained with essential oil and CA were not attenuated in the presence of nitric oxide synthase (NOS) inhibitor, and soluble guanylate cyclase inhibitor (sGS) in CC. In vivo, erectile responses in diabetic rats were lower than in control rats, which was restored after intracavernosal injection of CEO and CA. CEO and CA improved erectile function and relaxation of isolated strips of rat CC and HCC by a NO/cGMP-independent mechanism. Further investigations are warranted to fully elucidate the restorative effects of CEO and CA on diabetic erectile dysfunction.


Subject(s)
Humans , Animals , Male , Aged , Penis/drug effects , Acrolein/analogs & derivatives , Oils, Volatile/pharmacology , Cinnamomum zeylanicum/chemistry , Muscle Relaxation/drug effects , Penis/physiopathology , Phenylephrine/pharmacology , Vasoconstrictor Agents/pharmacology , Acrolein/pharmacology , Penile Erection/drug effects , Penile Erection/physiology , Reproducibility of Results , Analysis of Variance , Rats, Sprague-Dawley , Phosphodiesterase 5 Inhibitors/pharmacology , Sildenafil Citrate/pharmacology , Erectile Dysfunction/physiopathology , Erectile Dysfunction/drug therapy , Middle Aged , Muscle Relaxation/physiology
3.
Int. braz. j. urol ; 44(3): 550-554, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-954056

ABSTRACT

ABSTRACT Objectives: Evaluate the demographic data, etiology, operative findings and results of surgical treatment of penile fracture (PF) in men who have sex with men(MSM) with emphasis on sexual complications. Materials and Methods: We studied 216 patients underwent surgical correction of PF at our hospital. Patients self-identified as MSM were followed for at least 6 months. Demographic data, presentation, operative findings, International Index of Erection Function - 5 (IIEF-5) and the Premature Ejaculation Diagnostic Tool. Results: Of 216 PF cases, 4 (1.8%) were MSM. All cases resulted from sexual activity and all patients reported using the "doggy style" position during anal intercourse. Unilateral or bilateral injury of corpus cavernosum was found in 2 patients each. One (25%) patient had complete urethral injury associated with bilateral corpus cavernosum lesion. During the follow-up period, all patients developed some type of sexual complication. One patient reported penile pain during intercourse. Another patient experienced low sexual desire and premature ejaculation. This patient was also dissatisfied with the aesthetic result of the surgical scar and complained about decreased penis size after surgery. The third case developed delayed ejaculation. The fourth patient experienced mild to moderate erectile dysfunction. This same patient presented with penile curvature. Finally, palpable fibrotic nodules in the operative area were observed in all cases. Conclusions: Sexual activity in the "doggy style" position was the commonest cause of PF in MSM. Sexual dysfunction is always present in gay man after surgery for PF. However, additional studies with larger samples should be coinducted.


Subject(s)
Humans , Male , Adult , Penis/injuries , Sexual Dysfunction, Physiological/etiology , Homosexuality, Male , Penis/physiopathology , Posture , Rupture/surgery , Rupture/complications , Rupture/physiopathology , Sexual Behavior , Sexual Dysfunction, Physiological , Penile Erection/physiology , Retrospective Studies , Risk Factors , Treatment Outcome , Middle Aged
4.
Int. braz. j. urol ; 44(3): 555-562, May-June 2018. tab, graf
Article in English | LILACS | ID: biblio-954046

ABSTRACT

ABSTRACT Introduction: The study was aimed to assess the presence of actual differences between the objective and the perceived magnitude of a curvature between patients affected by Peyronie's disease (PD) and congenital penile curvature (CPC). Materials and Methods: Wee analysed a cohort of 88 consecutive patients seeking medi- cal help for either CPC or PD. All patients were invited to provide a self-made drawing of their penis in erection in order to obtain self-provided description of the deformity. An objective measurement of the deformity was also performed drawing two intersecting lines through the center of the distal and proximal straight section of the penile shaft. Results: Our findings showed significant differences between patient self-estimation and the objective measurements of the penile angulation performed by trained experts, with only 32% of patients correctly assessing their own curvature. Overall, patients tended to overestimate (56%) their degree of curvature, but the results are different in patients with PD than those with CPC. In the 60 men (68%) who did not accurately assess their curvature, PD patients generally overestimated their curvature versus CPC patients (67% vs 16%). On the contrary CPC patients underestimated their curvature compared to PD (42% vs. 4%). Conclusion: In order to improve patients' satisfaction rates, the surgeon needs to take into consideration the patient's perception of the deformity when planning the type of surgical correction.


Subject(s)
Humans , Male , Adolescent , Adult , Aged , Young Adult , Penile Induration/pathology , Penis/abnormalities , Penis/pathology , Diagnostic Self Evaluation , Penile Induration/physiopathology , Penile Induration/psychology , Penis/physiopathology , Perception , Reference Values , Severity of Illness Index , Penile Erection/physiology , Multivariate Analysis , Middle Aged
5.
Braz. j. med. biol. res ; 51(3): e6329, 2018. graf
Article in English | LILACS | ID: biblio-889035

ABSTRACT

Recent evidence shows that chronic ethanol consumption increases endothelin (ET)-1 induced sustained contraction of trabecular smooth muscle cells of the corpora cavernosa in corpus cavernosum of rats by a mechanism that involves increased expression of ETA and ETB receptors. Our goal was to evaluate the effects of alcohol and diabetes and their relationship to miRNA-155, miRNA-199 and endothelin receptors in the corpus cavernosum and blood of rats submitted to the experimental model of diabetes mellitus and chronic alcoholism. Forty-eight male Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D), and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study the protein expression of endothelin receptors by immunohistochemistry and expression of miRNAs-155 and -199 in serum and the cavernous tissue. Immunostaining for endothelin receptors was markedly higher in the A, D, and AD groups than in the C group. Moreover, a significant hypoexpression of the miRNA-199 in the corpus cavernosum tissue from the AD group was observed, compared to the C group. When analyzing the microRNA profile in blood, a significant hypoexpression of miRNA-155 in the AD group was observed compared to the C group. The miRNA-199 analysis demonstrated significant hypoexpression in D and AD groups compared to the C group. Our findings in corpus cavernosum showed downregulated miRNA-155 and miRNA-199 levels associated with upregulated protein expression and unaltered mRNA expression of ET receptors suggesting decreased ET receptor turnover, which can contribute to erectile dysfunction in diabetic rats exposed to high alcohol levels.


Subject(s)
Animals , Male , Rats , Alcoholism/metabolism , Diabetes Mellitus, Experimental/metabolism , Endothelin-1/analysis , MicroRNAs/analysis , Penis/metabolism , Receptor, Endothelin A/analysis , Receptor, Endothelin B/analysis , Alcoholism/complications , Alcoholism/physiopathology , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/physiopathology , Immunohistochemistry , Penis/physiopathology , Rats, Wistar
6.
Int. braz. j. urol ; 43(4): 753-761, July-Aug. 2017. tab, graf
Article in English | LILACS | ID: biblio-892855

ABSTRACT

ABSTRACT Objective Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. Materials and methods Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified. Results The weight, but not the length, of the penis was lower in diabetics. The cross-sectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls. Conclusions Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.


Subject(s)
Animals , Male , Rats , Penis/physiopathology , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Type 1/complications , Erectile Dysfunction/etiology , Muscle, Smooth/physiopathology , Penis/innervation , Rats, Sprague-Dawley , Diabetes Mellitus, Experimental/physiopathology , Diabetes Mellitus, Type 1/physiopathology , Nitric Oxide Synthase Type I/metabolism , Erectile Dysfunction/physiopathology
7.
Acta cir. bras ; 32(1): 38-45, Jan. 2017. graf
Article in English | LILACS | ID: biblio-837673

ABSTRACT

Abstract Purpose: To evaluate the expression of endothelial and inducible NOS in addition to the miRNA-27b in the corpus cavernosum and peripheral blood of healthy rats, diabetic rats, alcoholic rats and rats with both pathologies. Methods: Forty eight Wistar rats were divided into four groups: control (C), alcoholic (A), diabetic (D) and alcoholic-diabetic (AD). Samples of the corpus cavernosum were prepared to study protein expressions of eNOS and iNOS by immunohistochemistry and expression of miRNA-27b in the corpus cavernosum and peripheral blood. Results: Immunohistochemistry for eNOS and iNOS showed an increase in cavernosal smooth muscle cells in the alcoholic, diabetic and alcoholic-diabetic groups when compared with the control group. Similarly, the mRNA levels for eNOS were increased in cavernosal smooth muscle (CSM) in the alcoholic, diabetic and alcoholic-diabetic groups and miRNA-27b were decreased in CSM in the alcoholic, diabetic and alcoholic-diabetic groups. Conclusion: The major new finding of our study was an impairment of relaxation of cavernosal smooth muscle in alcoholic, diabetic, and alcoholic-diabetic rats that involved a decrease in the nitric oxide pathway by endothelium-dependent mechanisms accompanied by a change in the corpus cavernosum contractile sensitivity.


Subject(s)
Animals , Male , Rats , Penis/chemistry , MicroRNAs/analysis , Diabetes Mellitus, Experimental/metabolism , Alcoholism/metabolism , Nitric Oxide Synthase Type II/analysis , Nitric Oxide Synthase Type III/analysis , Penis/physiopathology , Immunohistochemistry , Rats, Wistar , Diabetes Mellitus, Experimental/complications , Diabetes Mellitus, Experimental/physiopathology , Alcoholism/complications , Alcoholism/physiopathology
9.
Korean Journal of Urology ; : 722-728, 2015.
Article in English | WPRIM | ID: wpr-128351

ABSTRACT

PURPOSE: The aim of this study was to compare the penile cuff test (PCT) and standard pressure-flow study (PFS) in patients with bladder outlet obstruction. MATERIALS AND METHODS: A total of 58 male patients with moderate to severe lower urinary tract symptoms (LUTS) were selected. Seven patients were excluded; thus, 51 patients were finally enrolled. Each of the patients underwent a PCT and a subsequent PFS. The sensitivity (SE), specificity (SP), positive predictive value (PPV), negative predictive value (NPV), and likelihood ratio were calculated. Chi-square and Fisher exact test were used to evaluate relationships between PCT results and maximal urine flow (Qmax); a p<0.05 was considered statistically significant. RESULTS: The mean (±standard deviation) age of the study group was 65.5±10.4 years. Overall, by use of the PCT, 24 patients were diagnosed as being obstructed and 27 patients as unobstructed. At the subsequent PFS, 16 of the 24 patients diagnosed as obstructed by the PCT were confirmed to be obstructed, 4 were diagnosed as unobstructed, and the remaining 4 patients appeared equivocal. Of the 27 patients shown to be unobstructed by the PCT, 25 were confirmed to not be obstructed by PFS, with 13 equivocal and 12 unobstructed. Two patients were diagnosed as being obstructed. For detecting obstruction, the PCT showed an SE of 88.9% and an SP of 75.7%. The PPV was 66.7% and the NPV was 93%. CONCLUSIONS: The PCT is a beneficial test for evaluating patients with LUTS. In particular, this instrument has an acceptable ability to reject obstruction caused by benign prostatic hyperplasia.


Subject(s)
Aged , Aged, 80 and over , Humans , Male , Middle Aged , Nomograms , Penis/physiopathology , Predictive Value of Tests , Pressure , Sensitivity and Specificity , Urinary Bladder Neck Obstruction/diagnosis , Urination/physiology , Urodynamics
10.
Korean Journal of Urology ; : 197-204, 2015.
Article in English | WPRIM | ID: wpr-60934

ABSTRACT

PURPOSE: Electroporation is known to enhance the efficiency of gene transfer through a transient increase in cell membrane permeability. The aim of this study was to determine the optimal conditions for in vivo electroporation-mediated gene delivery into mouse corpus cavernosum. MATERIALS AND METHODS: Diabetes was induced in C57BL/6 mice by intraperitoneal injections of streptozotocin. After intracavernous injection of pCMV-Luc (100 microg/40 microL), different electroporation settings (5-50 V, 8-16 pulses with a duration of 40-100 ms) were applied to the penis to establish the optimal conditions for electroporation. Gene expression was evaluated by luciferase assay. We also assessed the undesired consequences of electroporation by visual inspection and hematoxylin-eosin staining of penile tissue. RESULTS: Electroporation profoundly induced gene expression in the corpus cavernosum tissue of normal mice in a voltage-dependent manner. We observed electrical burn scars in the penis of normal mice who received electroporation with eight 40-ms pulses at a voltage of 50 V and sixteen 40-ms pulses, eight 100-ms pulses, and sixteen 100-ms pulses at a voltage of 30 V. No detectable burn scars were noted in normal mice stimulated with eight 40-ms pulses at a voltage of 30 V. Electroporation also significantly induced gene expression in diabetic mice stimulated with 40-ms pulse at a voltage of 30 V without injury to the penis. CONCLUSIONS: We have established the optimal electroporation conditions for maximizing gene transfer into the corpus cavernosum of mice while avoiding damage to the erectile tissue. The electroporation-mediated gene delivery technique will be a valuable tool for gene therapy in the field of erectile dysfunction.


Subject(s)
Animals , Diabetes Mellitus, Experimental/complications , Electroporation/methods , Erectile Dysfunction/therapy , Gene Expression , Gene Transfer Techniques , Genes, Reporter , Genetic Therapy/methods , Luciferases/metabolism , Male , Mice , Mice, Inbred C57BL , Penile Erection/physiology , Penis/physiopathology , Transfection
11.
Int. braz. j. urol ; 36(4): 490-496, July-Aug. 2010. ilus
Article in English | LILACS | ID: lil-562115

ABSTRACT

PURPOSE: To describe a technique for en bloc harvesting of the corpus cavernosum, cavernous artery and urethra from transplant organ donors and contraction-relaxation experiments with corpus cavernosum smooth muscle. MATERIALS AND METHODS: The corpus cavernosum was dissected to the point of attachment with the crus penis. A 3 cm segment (corpus cavernosum and urethra) was isolated and placed in ice-cold sterile transportation buffer. Under magnification, the cavernous artery was dissected. Thus, 2 cm fragments of cavernous artery and corpus cavernosum were obtained. Strips measuring 3 x 3 x 8 mm3 were then mounted vertically in an isolated organ bath device. Contractions were measured isometrically with a Narco-Biosystems force displacement transducer (model F-60, Narco-Biosystems, Houston, TX, USA) and recorded on a 4-channel Narco-Biosystems desk model polygraph. RESULTS: Phenylephrine (1µM) was used to induce tonic contractions in the corpus cavernosum (3 - 5 g tension) and cavernous artery (0.5 - 1g tension) until reaching a plateau. After precontraction, smooth muscle relaxants were used to produce relaxation-response curves (10-12M to 10-4 M). Sodium nitroprusside was used as a relaxation control. CONCLUSION: The harvesting technique and the smooth muscle contraction-relaxation model described in this study were shown to be useful instruments in the search for new drugs for the treatment of human erectile dysfunction.


Subject(s)
Adult , Aged , Humans , Male , Middle Aged , Endothelium, Vascular/physiology , Erectile Dysfunction/surgery , Muscle Relaxation/physiology , Muscle, Smooth/physiology , Penis/surgery , Erectile Dysfunction/physiopathology , Models, Theoretical , Penis/innervation , Penis/physiopathology
12.
Rio de Janeiro; s.n; 2009. 58 p. ilus.
Thesis in Portuguese | LILACS | ID: lil-564727

ABSTRACT

Introdução: Embora a disfunção erétil seja um problema frequente em pacientes com diabete mellitus, poucos estudos avaliaram as alterações da estrutura peniana nesta condição. Objetivo: Avaliar a densidade volumétrica de colágeno, fibras do sistema elástico e células musculares lisas no corpo cavernoso (CC), corpo esponjoso (CE) e túnica albugínea (TA) no pênis de coelhos diabéticos. Método: Vinte seis coelhos Nova Zelândia foram utilizados. A diabete foi induzida em 13 coelhos com 8 semanas de idade com injeção intravenosa de 100 mg/kg de aloxano. 13 colehos normais serviram como controle. Após 10 semanas os animais foram mortos com overdose de thiopenthal sódico endovenoso. Os pênis foram dissecados retirando-se um fragmento da porção média sendo fixado em formol 10% tamponado (pH 7.3). Todo material foi processado para inclusão em parafina seguindo a técnica histológica de rotina. Principais medidas: A análise estereológica do colágeno, das fibras do sistema elástico e do músculo liso foi realizada em cortes de 5um no sistema teste M42. Os dados foram expressos em densidade volumétrica (Vv %). A organização de colágeno foi avaliada pela coloração do Picrosirius red sob polarização. Resultados: na túnica albugínea de coelhos diabéticos, houve um aumento de 88% da espessura (p<0.0003) com um acentuado turnover do colágeno. Além disso, o conteúdo de fibras elásticas foi 34% maior (p<0.0001). No CC dos coelhos diabéticos o colágeno diminuiu 45% (p<0.0001) com a presença de um colágeno mais organizado. As fibras do sistema elástico tiveram um decréscimo de 46% (p<0.0001). A diabete induziu um aumento de 11% de colágeno no CE (P<0.0235) com um acentuado turnover do colágeno. No CC de coelhos diabéticos houve um aumento de 40% de músculo liso (p<0.0001), enquanto no CE uma diminuição de 38% (p<0.0001). Conclusão: Os tecidos do pênis foram afetados de formas diferentes pela diabete, possivelmente devido à heterogeneidade celular...


Introdution: Although erectile dysfunction is a frequent problem in patients with diabetes mellitus, few studies have evaluated penile structure alteration in this condition. Aim: To assess the volumetric density of collagen, elastic system fibers and smooth muscles cells in the corpora cavernosa (CC), corpus spongiosum (CE) and tunica albuginea (TA) in the penis of diabetic rabbits. Methods: Twenty-six New Zealand white rabbits were used. Diabetes was induced at 8 weeks of age in 13 rabbits by intravenous injection of 100 mg/kg of alloxan. The remaining 13 rabbits served as a control group. After 10 weeks, the rabbits were killed using sodium thiopenthal. Mid-shaft fragments of the penis were obtained and processed by routine histological techniques. Main Outcome Measures: Stereological analysis of collagen, elastic system fibers and smooth muscle was performed in 5-um sections by using a M42 System Test grid. Data were expressed as volumetric density (Vv - %). Collagen organization was evaluated by Picrosirius red staining under polarization. Results: In the TA of diabetic rabbits, thickness increased by 88% (p<0.0003) with an enhanced collagen turnover. Moreover, the elastic fiber content was 34% higher (p<0.0001). In the CC of diabetics, collagen was diminished by 45% (p<0.0001) with a more organized collagen. The elastic fibers were decreased by 46% (p<0.0001). Diabetes induced a 11% increase in CS collagen (p<0.0235) with an enhanced collagen turnover. Smooth muscle in the CC of diabetic rabbits was increased by 40% (p<0.0001), whereas in the CS it was decreased by a similar amount (p<0.0001). Conclusion: Penile tissues were affected differently by diabetes, possibly due to cellular heterogeneity. These changes could have an impact on blood flow and tissue resistance, and therefore, might adversely affect erection...


Subject(s)
Animals , Rabbits , Diabetes Complications , Diabetes Mellitus, Experimental/physiopathology , Erectile Dysfunction/etiology , Muscle, Smooth/physiopathology , Penis/physiopathology , Penis/blood supply , Connective Tissue/physiopathology , Elastic Tissue/anatomy & histology , Elastic Tissue/metabolism , Alloxan
14.
Yonsei Medical Journal ; : 29-33, 2000.
Article in English | WPRIM | ID: wpr-41099

ABSTRACT

SS-cream made with extracts from natural products is a topical agent for treating premature ejaculation (PE). In order to elucidate the penile vibratory threshold changes and clinical effects of various doses of SS-cream, 53 patients with primary PE were investigated in a double-blind randomized placebo-controlled study. The mean age was 37.3 +/- 6.4 years and mean ejaculatory latency was 1.37 +/- 0.52 minutes. Neither the patients nor their sexual partners were satisfied with their sexual lives. Vibratory threshold at the glans penis, penile shaft, scrotum and index finger were measured using a biothesiometer twice during the screening period and three times one hour after the application of respective creams (SS-cream 0.05, 0.10. 0.15, 0.20 gm and placebo 0.10 gm) on the glans penis according to the order of the allocation table in a randomized fashion. The efficacy of SS-cream was defined as when the vibration threshold increased by more than 4 microns compared to the value tested during the screening period. The vibratory thresholds at the glans penis increased significantly in a dose-dependent manner after the application of various doses (0.05, 0.10, 0.15, 0.20 gm) of SS-cream (p < 0.001), and the efficacy of SS-cream on the penile vibration threshold increased according to the increased dosage (penile shaft: 48.4, 51.6, 54.8, 64.5%, glans penis: 58.1, 67.7, 77.4, 83.9%, respectively). With these results, we concluded that SS-cream increased the penile sensory threshold dose dependently, and therefore it is clinically effective for treating the heightened penile sensory response in patients with PE.


Subject(s)
Adult , Double-Blind Method , Ejaculation/drug effects , Humans , Male , Penis/physiopathology , Plant Extracts/therapeutic use , Plants, Medicinal , Sensory Thresholds , Sexual Dysfunction, Physiological/physiopathology , Sexual Dysfunction, Physiological/drug therapy , Time Factors , Vibration
15.
Rev. argent. urol. (1990) ; 64(1): 9-17, ene.-mar. 1999. ilus
Article in Spanish | LILACS | ID: lil-239571

ABSTRACT

Correlación cavernosográfica de un hallazgo (drenaje continuo de la vena dorsal profunda del pene), que esclarece el conocimiento del "mal denominado" Plexo de Santorini al definir sus bases morfológicas reales. Se realizaron disecciones en cadáveres adultos para investigar el drenaje venoso del plexo de estudio, y se sistematizaron sus afluentes y relaciones. En pacientes con disfunción eréctil seleccionados se efectuaron cavernosográfias, y se sumaron archivos radiográficos. Observamos que: a) la región del supuesto Plexo de Santorini presenta un conjunto de plexos en relación con los órganos subyacentes; b) tiene como colector principal una rama de bifurcación emergente de la vena dorsal profunda del pene, que continua y finaliza formando la vena pudenda interna; c) el patrón cavernosográfico acorde con este hallazgo es constante, no varía con el grado de disfunción, su correlación con su morfología y ubicación. En conclusión: 1) el supuesto Plexo de Santorini es un conjunto de plexos distribuidos según la ubicación de los órganos de la región y de las paredes que lo contienen, sistematizados por el hallazgo y su patrón cavernmosográfico; 2) la vena dorsal profunda del pene no finaliza en el Plexo de Santorini, sino que a trtavés de su rama de bifurcación y su continuidad forma un eje colector que " ordena " el drenaje venosos de la región; 3) esta visible y constante ruta colectora mo sería sinónimo de patrón disfuncional a diferenciar en las cavernosografías


Subject(s)
Humans , Male , Erectile Dysfunction , Penis , Penis/anatomy & histology , Penis/physiopathology
17.
Medical Journal of the Islamic Republic of Iran. 1996; 10 (2): 113-115
in English | IMEMR | ID: emr-42062

ABSTRACT

A 14 year old boy suffering from marked penoscrotal lymphedema is reported. Reduction scrotoplasty was done and the neoscrotum was made by combining the anterior suprapubic and posterior scrotal U-shaped flaps. A split thickness skin graft from the thigh was placed over the denuded penis. No complication was encountered and the cosmetic result was satisfactory


Subject(s)
Penis/physiopathology
18.
Acta pediátr. Méx ; 16(2): 73-80, mar.-abr. 1995. ilus, tab
Article in Spanish | LILACS | ID: lil-173791

ABSTRACT

En el presente trabajo se analizaron los expedientes de 63 casos de hipospadias severos, corregidos mediante injerto autólogo de piel o mucosa vesical, con la técnica de Horton-Devine, en el periodo de 1986 a 1993 en el servicio de Urología del Instituto Nacional de Pediatría. La suma de porcentajes de fístulas, divertículos y estenosis, alcanzó el 85 por ciento de todos los casos, resultado distinto y en mayor proporción a las series publicadas. Analizados los pacientes con estímulo hormonal preoperatorio, comparados con aquellos en los que no se usaron hormonas, sus porcentajes de complicaciones disminuyeron significativamente. El resultado estético final fue entre excelente y bueno en 84 por ciento de los casos. Se concluye que aunque es un procedimiento que ofrece excelentes resultados estéticos y funcionales, amerita una segunda operación en el 85 por ciento de los casos


Subject(s)
Hypospadias/surgery , Hypospadias/therapy , Penis/physiopathology , Surgical Procedures, Operative , Testosterone/therapeutic use , Transplantation, Autologous , Urethral Stricture/complications
19.
Rev. argent. urol. (1990) ; 60(4): 159-66, 1995. ilus, tab
Article in Spanish | LILACS | ID: lil-181504

ABSTRACT

Describimos los hallazgos electromiográficos de 44 pacientes con lesión en la inervación autonómica del músculo liso cavernoso. Se utilizaron dos electromiógrafos adaptados para esa función, un polígrafo ATI 900 y un electromiógrafo de musculo liso Wiest. Las lesiones neurológicas fueron causadas en 17 pacientes por sección traumàtica de la uretra membranosa, en 25 por prostactectomia radical y 2 por resección abdominoperineal de recto (operación de Miles). La electromiografia de los cuerpos cavernosos EMG-CC permitió diagnosticar las lesiones de cada nervio eringente. Se observó que luego de un tiempo las lesiones completas pueden originar un registro compatible con una lesión degenerativa del músculo liso cavernoso causado por la falta de estimulación cavernosa basal. Se efectuaron registros simultáneos de la actividad simpática periférica de palmas y rótula con electrodos de superficio y coaxiales, correlacionándolos con la EMG-CC. Los pacientes con trastornos degenerativos del musculo liso cavernoso por lesión nerviosa presentaron una respuesta simpática epidérmica normal y registros de baja amplitud con respuesta escasa o ausente en los cuerpos cavernosos, aún ante maniobras de estimulación simpática. Se destaca la importancia de mantener farmacológicamente activo al musculo cavernoso, lo más tempranamente posible a la lesión neurológica


Subject(s)
Humans , Male , Adult , Middle Aged , Electromyography , Erectile Dysfunction/physiopathology , Erectile Dysfunction/psychology , Muscle, Smooth/innervation , Urethra/injuries , Penis/physiopathology
20.
Article in English | WPRIM | ID: wpr-218196

ABSTRACT

This study was intended to explore the effects of endothelium-independent, direct smooth muscle relaxants(papaverine, verapamil) and endothelium-dependent vasodilators(acetylcholine, bradykinin, adenosine) on the isolated cavernosal smooth muscle strips taken from diabetic men with impotence. When smooth muscle contraction was evoked with norepinephrine for the study of relaxation to these vasodilators, the tension induced was similar in diabetic and non-diabetic men with importance. Papaverine showed the strongest relaxation response followed by verapamil, acetylcholine, bradykinin and adenosine both in non-diabetic and in diabetic men. Relaxation of the cavernosal tissues to endothelium-independent vasodilators was similar in non-diabetic and diabetic men. However, the relaxation response of the tissues to endothelium-dependent vasodilators was significantly reduced in the diabetic group compared with that in the non-diabetic group (p<0.05). In conclusion, the impairment of endothelium-mediated relaxation of cavernosal smooth muscle seems to play a more important role in the pathogenesis of diabetogenic impotence rather than the problem of smooth muscle itself. This finding forms a rational basis for the use of intracavernosal injections of vasodilators to induce endothelium-independent relaxation of the cavernosal smooth muscle in the patients with diabetogenic impotence.


Subject(s)
Adult , Comparative Study , Diabetes Mellitus/physiopathology , Endothelium, Vascular/physiology , Humans , Erectile Dysfunction/physiopathology , In Vitro Techniques , Male , Middle Aged , Muscle Relaxation/drug effects , Muscle, Smooth/drug effects , Penis/physiopathology , Vasodilator Agents/pharmacology
SELECTION OF CITATIONS
SEARCH DETAIL