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1.
Article in English | WPRIM | ID: wpr-16097

ABSTRACT

A survey was performed to investigate the infection status of zoonotic trematode (ZT) metacercariae in fish from a local market in Yangon City, Myanmar. A total of 264 fish (12 species) were collected through 4 times from December 2013 to June 2015. All collected fish were transferred to our laboratory on ice and examined by the artificial digestion method. More than 7 species of ZT metacercariae, i.e., Haplorchis taichui, H. pumilio, H. yokogawai, Centrocestus spp., Stellantchasmus falcatus, Pygidiopsis cambodiensis, and Procerovum sp. were detected. Metacercariae of H. taichui were collected in 58 (42.3%) out of 137 fish (5 species), and their average density was 42.9 per fish infected. Metacercariae of H. pumilio were detected in 96 (49.0%) out of 196 fish (9 species), and their average density was 23.6 per fish infected. H. yokogawai metacercariae were found in 40 (50.0%) out of 80 fish (5 species), and Centrocestus spp. metacercariae in 91 (50.8%) out of 179 fish (8 species), and their densities were 306 and 25.8 per fish infected, respectively. Metacercariae of S. falcatus and P. cambodiensis were detected only in mullets, Chelon macrolepis. A total of 280 Procerovum sp. metacercariae were found in 6 out of 12 climbing perch, Anabas testudineus. Morphological characteristics of adult flukes recovered from experimental animals were described. It has been first confirmed that fish from Yangon, Myanmar are commonly infected with various species of ZT metacercariae.


Subject(s)
Adult , Animals , Digestion , Humans , Ice , Metacercariae , Methods , Myanmar , Perches , Smegmamorpha , Trematoda
2.
Article in English | WPRIM | ID: wpr-7493

ABSTRACT

A 46-year-old woman visited our hospital presenting throat pain and globus sensation. The symptoms occurred seven days after eating raw perch and mullet. An endoscopy under sedation showed a fluke―with an approximate length of 4.8 mm and width of 1.5 mm―on the left aryepiglottic fold, with active motility on the mucosa. It was extracted from the larynx using biopsy forceps and was identified as Clinostomum complanatum. To the best of our knowledge, this is the second reported case of human infection with Clinostomum complanatum diagnosed and treated by an endoscopy in Korea. Endoscopy is a useful tool in the diagnosis and treatment of patients at risk for parasitic infections complaining of throat pain.


Subject(s)
Biopsy , Diagnosis , Eating , Endoscopy , Female , Foodborne Diseases , Humans , Korea , Larynx , Middle Aged , Mucous Membrane , Parasites , Perches , Pharynx , Sensation , Smegmamorpha , Surgical Instruments
3.
Int. j. morphol ; 33(2): 751-758, jun. 2015. ilus
Article in English | LILACS | ID: lil-755539

ABSTRACT

The ultrastructure of the gastrointestinal tract of Nile perch was described using Transmission Electron Microscopy standard procedures. Investigations revealed the presence of mucous cells, blood vessels and oil droplets plus several nerve cells and muscle bundles in the oral cavity. Further observations revealed columnar epithelial cells in the oesophagus, with a ragged surface, high electron dense cytoplasm, intercellular spaces, mitochondria and mucus granules. The lamina propria of the oesophagus was composed of loose connective tissue, blood vessels, nerve cells and several leucocytes, while the muscularis externa was composed of striated muscle bundles with the a Z line, A band and I band. The stomach was characterized by mucosal glandular cells with electron dense granules, a prominent nucleus, mitochondria, and secretory canaliculi. The liver had an isotropic parenchyma composed of several hepatocytes with a single spherical euchromatic nucleus. The exocrine pancreatic cells in the liver occurred around large blood vessels, had large centrally positioned nuclei with electron dense nucleolus, electron dense granules, Rough Endoplasmic Reticulum, and mitochondria which dominated the cytoplasm of pancreatic cells.


La ultraestructura del tracto gastrointestinal de la perca del Nilo fue descrita utilizando un procedimiento estándar de Microscopía Electrónica de Transmisión (MET). Las investigaciones revelaron la presencia de células mucosas, vasos sanguíneos y pequeñas gotas de aceite, además de varias células nerviosas y haces musculares en la cavidad oral. Nuevas observaciones revelaron células epiteliales columnares en el esófago, con una superficie irregular, citoplasma elevado y electrodenso, espacios intercelulares, mitocondrias y gránulos mucosos. La lámina propia del esófago se compone de tejido conectivo laxo, vasos sanguíneos, células nerviosas y numerosos leucocitos, mientras que la muscular externa se compone de haces musculares estriados, con presencia de la línea Z, banda A y banda I. El estómago se caracteriza por presentar células glandulares mucosas con gránulos electrodensos , un núcleo prominente, mitocondrias y canalículos secretores. El hígado presentó un parénquima isotrópico compuesto de varios hepatocitos con un solo núcleo eucromático y esférico. Las células pancreáticas exocrinas se encontraron alrededor de grandes vasos sanguíneos, presentando grandes núcleos posicionados centralmente, con nucleólos densos, gránulos electrodensos en el retículo endoplasmático rugoso, y mitocondrias, que dominaron el citoplasma de las células pancreáticas.


Subject(s)
Animals , Gastrointestinal Tract/ultrastructure , Microscopy, Electron, Transmission , Perches/anatomy & histology
4.
Article in English | WPRIM | ID: wpr-163866

ABSTRACT

Diphyllobothrium latum infection in humans is not common in Republic of Korea. We report a case of fish tapeworm infection in a 10-year-old boy after ingestion of raw perch about 8 months ago. The patient complained of recurrent abdominal pain and watery diarrhea. A tapeworm, 85 cm in length, without scolex and neck, was spontaneously discharged in the feces of the patient. The patient was treated with 15-mg/kg single dose praziquantel, and follow-up stool examination was negative after one month. There was no evidence of relapse during the next six months.


Subject(s)
Abdominal Pain , Cestoda , Cestode Infections , Child , Diarrhea , Diphyllobothrium , Eating , Feces , Follow-Up Studies , Humans , Male , Neck , Perches , Praziquantel , Recurrence , Republic of Korea
5.
Int. j. morphol ; 31(3): 1068-1075, set. 2013. ilus
Article in English | LILACS | ID: lil-695002

ABSTRACT

The morphology of the gastrointestinal tract of adult Nile perch was described using standard SEM procedures. Investigations revealed the presence of cardiform teeth in the oral cavity, goblet cells and finger print-like microridges on the hard palate and oesophagus lumenal surface. Elaborate patterns and bacterial cells were observed on the stomach lumenal surface and intense foldings in the intestinal region. These observations provide a better understanding of the morphology of the gut in Nile perch and how it is suited for its digestive function.


El objetivo fue describir la morfología del tracto gastrointestinal de la perca del Nilo adulta mediante microscopía electrónica de barrido estándar. La investigación reveló la presencia de dientes cardiformes en la cavidad oral, células caliciformes y microcrestas como huellas digitales en el paladar duro y la superficie luminal del esófago. Se observaron patrones elaborados, así como bacterias en la superficie luminal del estómago, y plegamientos marcados en la región intestinal. Estas observaciones proporcionan una mejor comprensión de la morfología del intestino de la perca del Nilo y como se adapta para su función digestiva.


Subject(s)
Animals , Microscopy, Electron, Scanning , Perches/anatomy & histology , Gastrointestinal Tract/ultrastructure
6.
Int. j. morphol ; 29(4): 1429-1434, dic. 2011. ilus
Article in English | LILACS | ID: lil-627027

ABSTRACT

Morphological differences between two close fish populations were investigated in Doma Dam, north central Nigeria using principal component and discriminant analyses. The purpose was to identify the two species correctly and assign them into distinct genetic groups. To achieve this, seven morphometric measurements (body weight, standard length, total length, head length, body depth, dorsal fin length and caudal fin length) were made on each of the studied 252 fish specimens (153 Oreochromis niloticus and 99 Lates niloticus). The analysis of variance showed that significantly (P<0.05) higher values were recorded for Lates niloticus in all the body traits examined, except dorsal fin length. From the principal component analysis, the differences between the species resulted mainly from dorsal fin length, body weight, caudal fin length and head length. The stepwise discriminant analysis revealed that standard length, dorsal fin length and caudal fin length were the three most discriminating variables useful to distinguish clearly the two species at mature age. These three variables with strong discriminating power correctly classified 98.0 percent of individuals from the sample of known-fish populations. The classification accuracy was cross-validated using split-sample method, and indicated a 97.6 percent success rate (98.0 percent of Oreochromis niloticus and 97.0 percent of Lates niloticus were correctly assigned). The results can provide a sound scientific basis for the effective management and sustainable exploitation of the genetic resources of the two species under subtropical conditions.


Las diferencias morfológicas entre dos poblaciones de peces similares se han investigado en Doma Dam, centro-norte de Nigeria utilizando análisis de componentes principales y análisis discriminante. El propósito fue identificar correctamente las dos especies y asignarlas a los distintos grupos genéticos. Para lograr esto, siete medidas morfométricas (peso corporal, longitud estándar, longitud total, longitud de la cabeza, profundidad del cuerpo, longitud de la aleta dorsal y la longitud de la aleta caudal) se realizaron en cada una de las muestras de los 252 peces estudiados (153 Oreochromis niloticus y 99 Lates niloticus) . El análisis de varianza mostró que de forma significativa (p<0,05) los valores más altos se registraron en Lates niloticus, en todos los rasgos del cuerpo examinado con excepción de la longitud de la aleta dorsal. A partir del análisis de componentes principales, las diferencias entre las especies se observó principalmente en la longitud de la aleta dorsal, el peso corporal, la longitud de la aleta caudal y longitud de la cabeza. El análisis discriminante reveló que la longitud estándar, longitud aleta dorsal y caudal son las tres variables más discriminatorias de utilidad para distinguir claramente las dos especies en la edad madura. Estas tres variables con fuerte poder de discriminación clasificaron correctamente el 98,0 por ciento de los individuos de la muestra de las poblaciones conocidas de peces. La exactitud de la clasificación fue sometida a una validación cruzada utilizando el método de la muestra dividida, la que indicó una tasa de éxito del 97,6 por ciento (98,0 por ciento de Oreochromis niloticus y 97,0 por ciento de Lates niloticus asignados correctamente). Los resultados pueden proporcionar una base científica sólida para el manejo eficaz y la explotación sostenible de los recursos genéticos de las dos especies en condiciones subtropicales.


Subject(s)
Animals , Cichlids/anatomy & histology , Perches/anatomy & histology , Conservation of Natural Resources , Discriminant Analysis , Genotype , Multivariate Analysis , Nigeria , Principal Component Analysis , Perciformes/anatomy & histology , Perciformes/classification , Species Specificity
7.
Korean Journal of Medicine ; : 553-561, 2011.
Article in Korean | WPRIM | ID: wpr-68589

ABSTRACT

BACKGROUND/AIMS: Acute viral hepatitis A is a major health problem in Korea and the influx of genotype IIIA is thought to be one reason. We examined the differences in the clinical characteristics and laboratory findings of genotypes IA and IIIA in Daejeon. METHODS: From November 2009 to June 2010, 81 patients positive for IgM anti-HAV were enrolled prospectively. The hepatitis A was genotyped using real-time polymerase chain reaction. The clinical characteristics and laboratory results were compared on the basis of genotype. RESULTS: The mean patient age was 32.6 +/- 7.4 years. The mean hospitalization was 7.7 +/- 2.4 days. The patient occupation varied. Clinically, vomiting and diarrhea were relatively more prevalent in genotype IIIA than in IA. Abdominal pain and skin spots were relatively more prevalent in genotype IA than in IIIA. The hemoglobin, peak aspartate aminotransferase (AST) level, and C-reactive protein were statistically higher in genotype IIIA than in IA. The distributions of the peak AST, alanine aminotransferase (ALT) and total bilirubin values tended to be perched in genotype IIIA than in IA. The international normalized ratio (INR) tended to be slightly prolonged in genotype IIIA than in IA. CONCLUSIONS: Recently, genotype IIIA of acute viral hepatitis A has become prevalent in Daejeon. Hepatitis A genotype IIIA probably causes worse laboratory abnormalities than genotype IA.


Subject(s)
Abdominal Pain , Alanine Transaminase , Aspartate Aminotransferases , Bilirubin , C-Reactive Protein , Diarrhea , Genotype , Hemoglobins , Hepatitis , Hepatitis A , Hepatitis A Antibodies , Hospitalization , Humans , Immunoglobulin M , International Normalized Ratio , Korea , Occupations , Perches , Prevalence , Prospective Studies , Real-Time Polymerase Chain Reaction , Skin , Vomiting
8.
J Environ Biol ; 2007 Jan; 28(1): 141-3
Article in English | IMSEAR | ID: sea-113707

ABSTRACT

The present work reports oxygen uptake in relation to group size in the juveniles (body weight: 0.70 to 1.30 g) of a climbing perch, Anabas testudineus (Bloch). The experiments were conducted at 21.5 +/- 1.0 degrees C using a cylindrical glass respirometer having continuous water flow system. With an increase in the number of fish at an interval of 5 from 5 to 40 in each fourth subsequent experiment, the weight specific aquatic oxygen uptake of a fish decreased from 0.192 +/- 0.030 to 0.800 +/- 0.006 ml O2 x g(-1)x(h(-1). When compared with a controlled fish, there was a reduction of 27.80% in the oxygen uptake in a group of 5 but 58.39% in a group of 40. A negative and significant correlation (r = 0.8411, p < 0.01) was calculated between oxygen uptake and group size of fishes. The investigation showed that probably due to shoaling behaviour, the aquatic oxygen uptake in the juveniles of A. testudineus always remained high in controlled fish or a fish in isolation than when they were in a group.


Subject(s)
Animals , Behavior, Animal/physiology , Body Weight , Carbon Dioxide/analysis , Environment , Hydrogen-Ion Concentration , Oxygen/analysis , Perches/physiology , Population Density , Temperature
9.
Indian J Biochem Biophys ; 2006 Apr; 43(2): 119-22
Article in English | IMSEAR | ID: sea-27266

ABSTRACT

The short-term action of thyroid hormone tri-iodothyronine (T3) was studied in vivo and in vitro on antioxidant enzyme activities in a teleost Anabas testudineus (Bloch). T3 injection in vivo (200 ng) in normal fish decreased the lipid peroxidation products and increased superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) activities after 30 min. T3 in vitro (10(-6) M) increased the antioxidant activities of catalase, glutathione reductase (GR), GPx and glutathione level after 15/30 min, except SOD, substantiating in vivo effects in normal fish. The results suggest a rapid regulatory effect of thyroid hormone in vivo and in vitro, in the removal of reactive oxygen species in A testudineus.


Subject(s)
Animals , Antioxidants/metabolism , Catalase/metabolism , Enzyme Activation/physiology , Glutathione Peroxidase/metabolism , Glutathione Reductase/metabolism , Perches/metabolism , Superoxide Dismutase/metabolism , Triiodothyronine/physiology
10.
Braz. j. biol ; 63(1): 17-26, Feb. 2003. ilus, tab, graf
Article in English | LILACS | ID: lil-343402

ABSTRACT

The reproductive ecology of the freshwater fish Cichla monoculus Spix, 1831 (Osteichthyes: Cichlidae) was investigated in the Campo Grande Reservoir, Northeast Brazil. Rainfall, temperature, pH, dissolved oxygen and electrical conductivity of the water were recorded monthly. Fish collected on a monthly basis were measured, weighed, dissected, sexed and the stage of maturation of the gonads were assessed by macro and microscopic means. The semi-arid study region has short spells of rain of 2-3 months duration interspersed with dry seasons. A positive correlation was observed between rainfall and air and water temperatures and conductivity of the water. The study population had an extended spawning season, with peak reproductive activity coinciding with low water temperatures. Males were longer and heavier than females on average and were larger at onset of sexual maturity. The size frequency distributions of the oocytes indicate that C. monoculus is a multiple spawner with an estimated batch fecundity of 3100. Condition factor showed an inverse relationship in relation to gonad size during maturation in both sexes and spent fish were in poor condition. In mature males, lipid stores in the post-occipital cephalic protuberance, a secondary sexual characteristic developed during the reproductive phase, which depleted in spent individuals. The success of this fish is attributed to its reproductive capacity and to the phenotypic plasticity that allows it to adapt to the harsh ecological conditions that prevail in the semi-arid region of Northeast Brazil


Subject(s)
Animals , Male , Female , Ecology , Gonads , Perches , Reproduction , Sexual Maturation , Electric Conductivity , Environment , Fresh Water , Hydrogen-Ion Concentration , Perches , Seasons , Temperature
11.
Braz. j. biol ; 62(4a): 591-594, Nov. 2002. ilus
Article in English | LILACS | ID: lil-335614

ABSTRACT

This paper reports on Luidia senegalensis being followed by Diplectrum formosum and D. radiale in shallow soft bottoms of southeastern Brazilian coast, and comments on this association between bottom-dwelling organisms and serranids. Following is likely due to the serranid's inquisitive behaviour, and both juvenile and adult sea basses acted as followers, feeding on disturbed or exposed prey due to sea star activities


Subject(s)
Animals , Feeding Behavior , Perches , Starfish , Behavior, Animal , Brazil
12.
Indian J Exp Biol ; 2002 Nov; 40(11): 1285-94
Article in English | IMSEAR | ID: sea-62649

ABSTRACT

Genotoxic effects of EMS have been assessed in fish, A. testudineus, using widely accepted cytogenetic protocols like chromosome aberrations, nuclear anomalies in red blood cells and abnormal sperm head morphology. In addition, gel electrophoretic protein profiles and total protein contents in nine selected tissues were analysed for evaluating their utility as potential indicators of genotoxicity. EMS not only caused chromosomal aberrations in somatic cells, nuclear anomalies in red blood cells, and increased incidence of sperm with abnormal head morphology, but also altered significantly both protein profiles and total protein contents in all tissues tested vis-à-vis suitable controls, indicating relevance of protein data in genotoxicity assessment.


Subject(s)
Animals , Chromosome Aberrations/chemically induced , Erythrocytes/drug effects , Ethyl Methanesulfonate/toxicity , India , Male , Micronucleus Tests , Mutagens/toxicity , Perches/genetics , Proteins/metabolism , Spermatozoa/abnormalities
13.
Journal of the Egyptian Society of Parasitology. 2002; 32 (1): 145-54
in English | IMEMR | ID: emr-59712

ABSTRACT

Forty-four cases of intestinal capillariasis were diagnosed; 37 females and 7 males with ages ranged from 10 to 65 years, however most of them were between 20-40 years. Twenty-six cases were from the northern part of Upper Egypt, particularly Beni-Swif and Menia governorates. The patients were presented with borborygmi, chronic diarrhea, vomiting, loss of weight, lower limb edema and electrolyte imbalance for durations ranging from one month to two years. The morbidity and mortality of the disease in Egyptian cases were described. Some patients gave history of eating raw whole fish, while others gave history of eating raw parts of fish or half-cooked fish. The diagnosis was based on the clinical picture and confirmed stool analysis. The differential diagnosis was discussed and the cost paid by patients to reach the correct diagnosis was estimated


Subject(s)
Humans , Male , Female , Parasitic Diseases , Fishes , Catfishes , Feeding Behavior , Diagnosis, Differential , Feces/parasitology , Intestinal Diseases, Parasitic , Perches , Epidemiologic Studies
14.
Braz. j. med. biol. res ; 34(6): 719-26, Jun. 2001. ilus, graf
Article in English | LILACS | ID: lil-285844

ABSTRACT

The livers of Geophagus brasiliensis collected from both a non-polluted site and a polluted site were analyzed for different antioxidant defenses, O2 consumption, thiobarbituric acid-reactive substance (TBARS) levels, and histological damage. Compared to controls (116.6 + or - 26.1 nmol g-1), TBARS levels were enhanced at the polluted site (284.2 + or - 25.6 nmol g-1), as also was oxygen consumption (86.6 + or - 11.3 and 128.5 + or - 9.8 µmol O2 min-1 g-1, respectively). With respect to enzymatic antioxidants, increased catalase activities (8.7 + or - 1.3 and 29.2 + or - 2.4 mmol min-1 g-1, respectively), unchanged superoxide dismutase activities (767.2 + or - 113.3 and 563.3 + or - 70.2 U g-1, respectively), and diminished glutathione S-transferase activities (29.0 + or - 3.2 and 14.9 + or - 3.2 µmol min-1 g-1, respectively) were detected. Reduced glutathione (1.91 + or - 0.17 and 1.37 + or - 0.25 mM, respectively), oxidized glutathione (1.50 + or - 0.20 and 0.73 + or - 0.17 mM, respectively), and total glutathione (3.40 + or - 0.26 and 2.07 + or - 0.27 mM, respectively) concentrations were also below control values at the polluted site. Nevertheless, the observed ethoxyresorufin-O-deethylase activities (1.34 + or - 0.11 and 16.7 + or - 0.21 pmol min-1 mg-1, respectively) showed enhanced values at the polluted site. The main histological damage observed in the hepatocytes from fish collected at the polluted site was characterized by heavy lipid infiltration. Fish collected at the end of spring showed higher O2 consumption, higher superoxide dismutase and glutathione S-transferase activities, and higher total and oxidized glutathione concentrations compared to the beginning of autumn. No seasonal changes were observed in catalase activities, glutathione or TBARS levels. Fish chronically exposed to relatively high pollution levels seem to be unable to set up adequate antioxidant defenses, probably due to severe injury to their hepatocytes. The higher antioxidant defenses found at the end of spring are probably related to the enhanced activities during high temperature periods in thermoconforming organisms.


Subject(s)
Animals , Male , Adaptation, Physiological , Antioxidants/metabolism , Perches/physiology , Seasons , Water Pollution , Glutathione/metabolism , Liver/metabolism , Liver/pathology , Oxidoreductases/metabolism , Oxygen Consumption
15.
Biocell ; 25(1): 35-42, Apr. 2001.
Article in English | LILACS | ID: lil-335886

ABSTRACT

The adenohypophysis of the cichlid fish Cichlasoma dimerus was studied using the avidin-biotin-peroxidase method with antisera raised against piscine pituitary hormones and heterologous antisera against mammalian pituitary hormones. Antiserum raised against rabbit ACTH recognized a group of cells bordering the neurohypophysis (NH) in the rostral pars distalis (RPD). Anti-chum salmon prolactin (PRL) identified a compact group of cells in the periphery of the RPD. Gonadotropin II (GTH II), thyrotropin (TSH) and growth hormone (GH)-ir cells were localized in the proximal pars distalis. Ir-GTH II cells were also observed in the dorsal area of the pars intermedia (PI). Ir-GTH I cells could not be identified using anti-chum salmon GTH I, this may be due either to a failure of the antisera to recognize the gonadotropin or to a low expression of the hormone in adults of this species. PAS positive cells from the PI bound specifically with three different antisera raised against somatolactin (SL) of four different fish species. These cells surrounded deep branches of the NH in the PI.


Subject(s)
Humans , Animals , Pituitary Gland, Anterior/chemistry , Pituitary Gland, Anterior/cytology , Perches , Adrenocorticotropic Hormone , Glycoproteins/analysis , Gonadotropins , Growth Hormone/analysis , Pituitary Hormones/analysis , Immunohistochemistry , Neurons/cytology , Prolactin , Thyrotropin
16.
J Environ Biol ; 2001 Apr; 22(2): 87-90
Article in English | IMSEAR | ID: sea-113238

ABSTRACT

Histopathological effects of sublethal doses of monocrotophos on the gills have been studied by exposing the fish for a period ranging from ten to twenty days. The extent of damage to gills was dependent on the dose and duration of exposure. Histopathological changes in the gills observed were characterized primarily by hemorrhage in the primary and secondary gill lemellae. Degeneration and necrosis of epithelial cells were very prominent. Distortion of the secondary lamellae was very prominent, beginning with disruption of epithelial cells from pillar cells.


Subject(s)
Animals , Dose-Response Relationship, Drug , Gills/blood supply , Hemorrhage , Insecticides/adverse effects , Monocrotophos/adverse effects , Perches , Time Factors , Water Pollutants, Chemical/adverse effects
17.
Mem. Inst. Oswaldo Cruz ; 94(1): 31-5, Jan.-Feb. 1999. ilus, tab, graf
Article in English | LILACS | ID: lil-225928

ABSTRACT

The mollucs Heleobia castellanosae and Ampullaria canaliculata and cichlid fishes were collected from an artificial pond at Tres de Febrero Park (Buenos Aires city), between June 1994 and May 1995. One hundred and eight of 2,467 H. castellanosae examined were infected with pre-adults of Lobatostoma jungwirthi, 112 of them in concurrent infections with digeneans. L. jungwirthi was significantly more prevalent in larger snails, specially those infected with larval digeneans, but the prevalence of infection did not fluctuate significantly throughout the year. A. canaliculata and cichlids smaller than 10 cm were not infected, but larger Cichlasoma facetum harboured adults of L. jungwirthi in their intestines. Experimental infections of C. facetum and Gymnogeophagus meridionalis with pre-adult aspidogastreans obtained from H. castellanosae were successful. The development of L. jungwirthi in the snail host involves five arbitrary growth phases. Its life-cycle is heteroxenous, similar to that described for L. manteri, with H. castellanosae as the intermediate host and cichlid fishes as the definitive host in this pond.


Subject(s)
Animals , Fishes/parasitology , Mollusca/parasitology , Perches/parasitology , Snails/parasitology , Trematoda , Argentina
18.
Indian J Exp Biol ; 1997 Aug; 35(8): 815-21
Article in English | IMSEAR | ID: sea-56837

ABSTRACT

Oocytes of vitellogenic stage were collected from A. testudineus and incubated in vitro for 4 hr in the absence (control) or presence of 500 ng of piscine gonadotropic hormone (GtH). After the termination of incubation, oocytes were repeatedly washed and then homogenized and ultracentrifuged at 100,000 g to obtain the supernatant fraction (100K sup). Addition of 100K sup from GtH treated oocytes to the oocyte incubation caused a 3-fold increase in ovarian aromatase activity as compared to the control, whereas 100K sup from control oocytes had no such stimulatory activity. Addition of cycloheximide (50 micrograms/ml) along with GtH blocked the stimulatory effect of 100K sup. Treatment of 100K sup from GtH incubate with pepsin or heat also destroyed its stimulatory effect. All these indicate proteinaceous nature of the factor. This factor was purified to 161-fold by utilizing Sephadex G-75 and DEAE Sephacel chromatography. Addition of increasing concentrations of partially purified GtH induced protein (GIP) to oocyte incubation caused a dose dependent increase in ovarian aromatase activity. Both dbcAMP and forskolin mimicked GIP activity. Results indicate that GtH induces the synthesis of a protein factor in perch oocytes which stimulates aromatase activity via the mediation of cAMP.


Subject(s)
Animals , Aromatase/metabolism , Egg Proteins/biosynthesis , Female , Gonadotropins/pharmacology , Oocytes/drug effects , Ovary/cytology , Perches , Stimulation, Chemical , Vitellogenesis/drug effects
19.
Article in Korean | WPRIM | ID: wpr-46176

ABSTRACT

The waste rates of 98 food items after pre-preparation were surveyed to provide database for good menu planning. The waste rates turned out 0~64.27% in vegetables, 6.38~7.03% in potatoes, 6.25~68.75% in fishes and shellfishes, 16.61% in eggs, and 16.00~56.84% in fruits. Foods with high waste rates were vegetables, fishes and shellfishes. Survey results were compared with other food composition tables. Foods with 30% higher waste rate than other food composition tables were squash leaf, pacific ocean perch, sweet potato stalk, water cress, green peas, alaska pollack, bluefin tuna, beka squid, crown daisy, dodok, amaranth, beef ribs. Food which waste rates turned out to be decreased by about 30% in this study were corb shell, pomfret, sea mussel, warty sea squirt. For the menu planning, reasonaly exact waste rates for each food items are essential. Since survey results show signigicant deviations, there should be more studies for exact waste rates for each food.


Subject(s)
Alaska , Bivalvia , Brassicaceae , Crowns , Decapodiformes , Eggs , Fishes , Fruit , Ipomoea batatas , Menu Planning , Ovum , Pacific Ocean , Peas , Perches , Ribs , Shellfish , Solanum tuberosum , Tuna , Vegetables
20.
Article in Korean | WPRIM | ID: wpr-194624

ABSTRACT

Human diphyllobolthriasis is chiefly caused by infection with Diphyllobothrium latum(D. latum) through eating raw or unproperly cooked fish such as salmon, trout or perch. D. latum is worldwidely distributed, but not a common parasite of humans, and its prevalence in Korea is not high. The first worm-proven case of D. latum infection was first reported in 1971 and about 30 cases have been reported in Korea until now. However, with the increasing popularity of eating raw salmon, trout and perch, this tapeworm infection tends to increase in Korea. Most of the patients infected by D. latum have not showed the specific symptoms and only non-specific gastrointestinal symptoms(anorexia, nausea, vomiting, diarrhea, abdominal pain). We report a case which includes a chain whitish, threadlike tapeworm segments spontaneously discharged in the patients stool. On reviewing the literature, the present case is the first report of D. latum infection by eating cultured salmon. The patient was treated with 600mg single dose of Praziquantel.


Subject(s)
Cestoda , Cestode Infections , Diarrhea , Diphyllobothrium , Eating , Humans , Korea , Nausea , Parasites , Perches , Praziquantel , Prevalence , Salmon , Trout , Vomiting
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