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1.
J. appl. oral sci ; 28: e20190215, 2020. graf
Article in English | LILACS, BBO | ID: biblio-1056582

ABSTRACT

Abstract Objective: This study evaluated the angiogenesis-enhancing potential of a tricalcium silicate-based mineral trioxide aggregate (ProRoot MTA), Biodentine, and a novel bioceramic root canal sealer (Well-Root ST) in human dental pulp stem cells (hDPSCs), human periodontal ligament stem cells (hPLSCs), and human tooth germ stem cells (hTGSCs). Methodology: Dulbecco's modified Eagle's medium was conditioned for 24 h by exposure to ProRoot MTA, Biodentine, or Well-Root ST specimens (prepared according to the manufacturers' instructions). The cells were cultured in these conditioned media and their viability was assessed with 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy-phenyl)-2-(4-sulfo-phenyl)-2H tetrazolium (MTS) on days 1, 3, 7, 10, and 14. Angiogenic growth factors [platelet-derived growth factor (PDGF), basic fibroblast growth factor (FGF-2), and vascular endothelial growth factor (VEGF)] were assayed by sandwich enzyme-linked immunosorbent assay (ELISA) on days 1, 7, and 14. Human umbilical vein endothelial cell (HUVEC) migration assays were used to evaluate the vascular effects of the tested materials at 6-8 h. Statistical analyses included Kruskal-Wallis, Mann-Whitney U, and Friedman and Wilcoxon signed rank tests. Results: None of tricalcium silicate-based materials were cytotoxic and all induced a similar release of angiogenic growth factors (PDGF, FGF-2, and VEGF) (p>0.05). The best cell viability was observed for hDPSCs (p<0.05) with all tricalcium silicate-based materials at day 14. Tube formation by HUVECs showed a significant increase with all tested materials (p<0.05). Conclusion: The tricalcium silicate-based materials showed potential for angiogenic stimulation of all stem cell types and significantly enhanced tube formation by HUVECs.


Subject(s)
Humans , Root Canal Filling Materials/pharmacology , Stem Cells/drug effects , Ceramics/pharmacology , Silicates/pharmacology , Calcium Compounds/pharmacology , Angiogenesis Inducing Agents/pharmacology , Periodontal Ligament/cytology , Periodontal Ligament/drug effects , Tooth Germ/cytology , Tooth Germ/drug effects , Biocompatible Materials/pharmacology , Materials Testing , Platelet-Derived Growth Factor/analysis , Platelet-Derived Growth Factor/drug effects , Enzyme-Linked Immunosorbent Assay , Cell Survival/drug effects , Reproducibility of Results , Fibroblast Growth Factor 2/analysis , Fibroblast Growth Factor 2/drug effects , Statistics, Nonparametric , Neovascularization, Physiologic/drug effects , Dental Pulp/cytology , Dental Pulp/drug effects , Vascular Endothelial Growth Factor A/analysis , Vascular Endothelial Growth Factor A/drug effects , Human Umbilical Vein Endothelial Cells/drug effects , Flow Cytometry
2.
J. appl. oral sci ; 27: e20180693, 2019. graf
Article in English | LILACS, BBO | ID: biblio-1040226

ABSTRACT

ABSTRACT Objectives: To compare the sealing ability and biocompatibility of Biodentine with mineral trioxide aggregate (MTA) when used as root-end filling materials. Methodology: The Cell Counting Kit-8 (CCK-8) assay was used to compare the cytotoxicity of MTA and Biodentine. Twenty-one extracted teeth with a single canal were immersed in an acidic silver nitrate solution after root-end filling. Then, the volume and depth of silver nitrate that infiltrated the apical portion of the teeth were analyzed using micro-computed tomography (micro-CT). Seventy-two roots from 3 female beagle dogs were randomly distributed into 3 groups and apical surgery was performed. After six months, the volume of the bone defect surrounding these roots was analyzed using micro-CT. Results: Based on the results of the CCK-8 assay, MTA and Biodentine did not show statistically significant differences in cytotoxicity (P>0.05). The volume and the depth of the infiltrated nitrate solution were greater in the MTA group than in the Biodentine group (P<0.05). The volume of the bone defect was larger in the MTA group than in the Biodentine group. However, the difference was not significant (P>0.05). The volumes of the bone defects in the MTA and Biodentine groups were smaller than the group without any filling materials (P<0.05). Conclusions: MTA and Biodentine exhibited comparable cellular biocompatibility. Biodentine showed a superior sealing ability to MTA in root-end filling. Both Biodentine and MTA promoted periradicular bone healing in beagle dog periradicular surgery models.


Subject(s)
Humans , Animals , Male , Adolescent , Dogs , Oxides/pharmacology , Periapical Tissue/drug effects , Periodontal Ligament/drug effects , Root Canal Filling Materials/pharmacology , Root Canal Therapy/methods , Wound Healing/drug effects , Silicates/pharmacology , Calcium Compounds/pharmacology , Aluminum Compounds/pharmacology , Osteogenesis/drug effects , Periapical Tissue/cytology , Periapical Tissue/diagnostic imaging , Periodontal Ligament/diagnostic imaging , Time Factors , Tooth Root/surgery , Tooth Root/drug effects , Tooth Root/diagnostic imaging , Bone Regeneration/drug effects , Materials Testing , Cell Count , Cells, Cultured , Reproducibility of Results , Treatment Outcome , Drug Combinations , X-Ray Microtomography
3.
Braz. oral res. (Online) ; 32: e7, 2018. tab, graf
Article in English | LILACS | ID: biblio-889497

ABSTRACT

Abstract The aim of this study is to evaluate the action of paramonochlorophenol associated with Furacin followed by calcium hydroxide (CH) dressing in the control of inflammatory root resorption in cases of immediate tooth replantation with delayed endodontic treatment. A total of 28 incisors of 3 male dogs were extracted and replanted after 15 minutes, and randomly divided into 3 groups: Group I (n = 8) - endodontic treatment was performed before the extraction and replantation; Group II (n = 10) - endodontic treatment was performed 30 days after replantation and the root canal was filled with CH dressing; Group III (n = 10) - endodontic treatment was performed 30 days after replantation and root canals received temporary medication of paramonochlorophenol-Furacin followed by CH dressing. The animals were euthanized 90 days after replantation. The histomorphological events analyzed at the epithelial reattachment site were the intensity and extent of acute and chronic inflammatory processes, periodontal ligament (PDL) organization, the intensity and extent of acute and chronic inflammatory processes in the PDL space, root resorption, bone tissue, and ankylosis. Data were submitted to the Wilcoxon Signed Ranks Test for group comparison (α = 5%). In Groups I, II and III the periodontal ligament was regenerated and most of the resorption areas were repaired by newly formed cementum. The depth and extent of root resorption were significantly higher in Group II than in Group III. The use of paramonochlorophenol-furacin followed by CH dressing was more effective in controlling inflammatory root resorption after immediate tooth replantation.


Subject(s)
Animals , Male , Dogs , Root Resorption/prevention & control , Tooth Replantation/methods , Chlorophenols/pharmacology , Tooth, Nonvital/drug therapy , Anti-Infective Agents, Local/pharmacology , Nitrofurazone/pharmacology , Periodontal Ligament/drug effects , Periodontal Ligament/pathology , Root Canal Filling Materials/pharmacology , Root Canal Therapy/methods , Root Resorption/pathology , Time Factors , Tooth Root/drug effects , Tooth Root/pathology , Calcium Hydroxide/pharmacology , Random Allocation , Reproducibility of Results , Treatment Outcome , Tooth, Nonvital/pathology , Medical Illustration
4.
J. appl. oral sci ; 25(6): 689-699, Nov.-Dec. 2017. graf
Article in English | LILACS, BBO | ID: biblio-893665

ABSTRACT

Abstract Periodontal regeneration is still a challenge in terms of predictability and magnitude of effect. In this study we assess the biological effects of combining chemical root conditioning and biological mediators on three relevant cell types for periodontal regeneration. Material and Methods: Bovine dentin slices were conditioned with 25% citric acid followed by topical application of basic fibroblast growth factor (bFGF, 10 and 50 ng). We used ELISA to assess the dynamics of bFGF release from the dentin surface and RT-qPCR to study the expression of Runx2, Col1a1, Bglap and fibronectin by periodontal ligament (PDL) fibroblasts, cementoblasts and bone marrow stromal cells (BMSC) grown onto these dentin slices. We also assessed the effects of topical application of bFGF on cell proliferation by quantification of genomic DNA. Results: Acid conditioning significantly increased the release of bFGF from dentin slices. Overall, bFGF application significantly (p<0.05) increased cell proliferation, except for BMSC grown on non-conditioned dentin slices. Dentin substrate discretely increased expression of Col1a1 in all cell types. Expression of Runx2, Col1a1 and Fn was either unaffected or inhibited by bFGF application in all cell types. We could not detect expression of the target genes on BMSC grown onto conditioned dentin. Conclusion: Acid conditioning of dentin improves the release of topically-applied bFGF. Topical application of bFGF had a stimulatory effect on proliferation of PDL fibroblasts, cementoblasts and BMSC, but did not affect expression of Runx2, Col1a1, Bglap and fibronectin by these cells.


Subject(s)
Animals , Cattle , Periodontal Ligament/drug effects , Regeneration/drug effects , Fibroblast Growth Factor 2/pharmacology , Dentin/drug effects , Cell Proliferation/drug effects , Periodontal Ligament/metabolism , Gene Expression , Fibroblast Growth Factor 2/administration & dosage
5.
J. appl. oral sci ; 25(2): 159-167, Mar.-Apr. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-841178

ABSTRACT

Abstract Reports have indicated that serotonin plays an important role in cell migration and differentiation during the organogenesis of several tissues, including the oral types. Administration of selective serotonin reuptake inhibitor (SSRI) drugs during pregnancy could affect the delivery of serotonin to embryonic tissues altering its development. Objective This study aimed to assess the effects of fluoxetine, a selective serotonin reuptake inhibitor, on the formation of the periodontal ligament during pregnancy and lactation in rat pups. Material and Methods Twelve pregnant rats of Wistar lineage were divided into four study groups. In the control group, 0.9% sodium chloride solution was administered orally, throughout the entire period of the 21 days of pregnancy (CG group) and in the CGL group, it was administrated during pregnancy and lactation (from day 1 of pregnancy to the 21st day after birth). Fluoxetine was administered orally at the dose of 20 mg/kg in a group treated during pregnancy only (FG group), and during pregnancy and lactation (FGL group). Histometrical, histochemical and immunohistochemical analysis of the maxillary first molar periodontium region of the 24 rat pups was made under light microscopy, and periodontal ligament collagen was qualitatively evaluated under a polarizing light microscope. Results The quantity of fibroblasts (p=0.006), osteoblasts (p=0.027) and cementoblasts (p=0.001) was reduced in pups from the rats that received fluoxetine during pregnancy and lactation. No alterations were seen in the collagen fibers. Conclusion These findings suggest that periodontal tissue may be sensitive to fluoxetine, and its interference in reducing periodontal cells depends on exposure time during lactation.


Subject(s)
Animals , Male , Female , Pregnancy , Osteogenesis/drug effects , Periodontal Ligament/drug effects , Fluoxetine/pharmacology , Selective Serotonin Reuptake Inhibitors/pharmacology , Periodontal Ligament/growth & development , Periodontal Ligament/embryology , Time Factors , Lactation , Immunohistochemistry , Random Allocation , Rats, Wistar , Maternal Exposure , Fibrillar Collagens/analysis , Alveolar Process/drug effects , Alveolar Process/embryology
6.
Dental press j. orthod. (Impr.) ; 21(2): 45-50, Mar.-Apr. 2016. tab
Article in English | LILACS | ID: lil-782946

ABSTRACT

ABSTRACT Introduction: Osteoclasts and osteoblasts are responsible for regulating bone homeostasis during which the trace element zinc has been shown to exert a cumulative effect on bone mass by stimulating osteoblastic bone formation and inhibiting osteoclastic bone resorption. Objective: The aim of the present study was to investigate the effects of zinc (Zn) on orthodontic tooth movement (OTM) in a rat model. Material and Methods: A total of 44 male Wistar rats were divided into four groups of 11 animals each and received 0, 1.5, 20 and 50 ppm Zn in distilled water for 60 days. In the last 21 days of the study, nickel-titanium closed coil springs were ligated between maxillary right incisors and first molars of all rats, and tooth movement was measured at the end of this period. Histological analysis of hematoxylin/eosin slides was performed to assess root resorption lacunae, osteoclast number and periodontal ligament (PDL) width. Results: Mean OTM was calculated as 51.8, 49.1, 35.5 and 45 µm in the 0, 1.5, 20 and 50 ppm zinc-receiving groups, respectively. There were no significant differences in neither OTM nor histological parameters among the study groups (p > 0.05). Conclusion: According to the results obtained in the current investigation, increase in supplementary zinc up to 50 ppm does not affect the rate of OTM neither bone and root resorption in rats.


RESUMO Introdução: os osteoclastos e os osteoblastos são responsáveis por regular a homeostase óssea, processo no qual o oligoelemento zinco tem demonstrado exercer um efeito cumulativo sobre a massa óssea, estimulando a formação óssea osteoblástica e inibindo a reabsorção óssea osteoclástica. Objetivo: o objetivo do presente estudo foi investigar os efeitos do zinco (Zn) sobre a movimentação dentária ortodôntica (MDO) em ratos. Métodos: um total de 44 ratos Wistar machos foi dividido em quatro grupos de 11 animais cada, os quais receberam 0; 1,5; 20 e 50ppm de zinco diluído em água destilada, durante 60 dias. Nos últimos 21 dias do estudo, molas helicoidais fechadas de níquel-titânio foram instaladas entre os incisivos direitos e os primeiros molares superiores de todos os ratos, e a movimentação dentária foi medida ao final desse período. Foi realizada análise histológica de cortes corados por hematoxilina-eosina, para avaliar as lacunas de reabsorção radicular, o número de osteoclastos e a espessura do ligamento periodontal. Resultados: as médias da MDO foram estimadas em 51,8; 49,1; 35,5 e 45µm no grupos que receberam, respectivamente, 0; 1,5; 20 e 50ppm de zinco. Não houve diferença significativa entre os grupos experimentais, nem quanto à MDO, nem quanto aos parâmetros histológicos (p > 0,05). Conclusão: segundo os resultados obtidos na presente investigação, verificou-se que um aumento na dose de suplementação com zinco para 50ppm não afeta nem o índice de MDO, nem a reabsorção óssea ou radicular em ratos.


Subject(s)
Animals , Male , Rats , Osteoblasts/drug effects , Tooth Movement Techniques/methods , Zinc/administration & dosage , Bone Resorption/prevention & control , Osteoblasts/pathology , Periodontal Ligament/drug effects , Periodontal Ligament/pathology , Bone Resorption/pathology , Rats, Wistar , Dose-Response Relationship, Drug
7.
Braz. j. med. biol. res ; 49(8): e5291, 2016. tab, graf
Article in English | LILACS | ID: lil-787385

ABSTRACT

Fluoride, which is often added to toothpaste or mouthwash in order to protect teeth from decay, may be a novel therapeutic approach for acceleration of periodontal regeneration. Therefore, we investigated the effects of fluoride on proliferation and mineralization in human periodontal ligament cells in vitro. The periodontal ligament cells were stimulated with various concentrations of NaF added into osteogenic inductive medium. Immunohistochemistry of cell identification, cell proliferation, alkaline phosphatase (ALP) activity assay, Alizarin red S staining and quantitative real-time-polymerase chain reaction (RT-PCR) were performed. Moderate concentrations of NaF (50-500 μmol/L) had pro-proliferation effects, while 500 μmol/L had the best effects. ALP activity and calcium content were significantly enhanced by 10 μmol/L NaF with osteogenic inductive medium. Quantitative RT-PCR data varied in genes as a result of different NaF concentrations and treatment periods. We conclude that moderate concentrations of NaF can stimulate proliferation and mineralization in periodontal ligament cells. These in vitro findings may provide a novel therapeutic approach for acceleration of periodontal regeneration by addition of suitable concentrations of NaF into the medication for periodontitis treatment, i.e., into periodontal packs and tissue patches.


Subject(s)
Humans , Child , Adolescent , Adult , Young Adult , Cell Proliferation/drug effects , Periodontal Ligament/drug effects , Sodium Fluoride/pharmacology , Alkaline Phosphatase/drug effects , Alkaline Phosphatase/metabolism , Calcium/metabolism , Cells, Cultured/drug effects , Periodontal Ligament/cytology , Real-Time Polymerase Chain Reaction/methods
8.
Braz. oral res. (Online) ; 30(1): e74, 2016. tab, graf
Article in English | LILACS | ID: biblio-952057

ABSTRACT

Abstract Revascularization of immature teeth with necrotic pulps traditionally involves the use of triple antibiotic paste, which may sometimes lead to undesirable complications. The objective of this study was to assess tissue repair in immature dog teeth with apical periodontitis subjected to revascularization, comparing two different pastes used for root canal disinfection. Apical periodontitis was induced in 30 dog premolars. Teeth were randomly divided into three experimental groups: root canals filled with triple antibiotic paste (n = 10); root canals filled with 1% propolis paste (n = 10); and no medication (n = 10). An additional group (n = 10, no intervention) was used as control. After 7 months, the jaws were histologically evaluated for the following variables: newly formed mineralized tissue (present/absent); vital tissue in the canal space (absent/periodontal ligament-like/pulp-like); apical extension of root (present/absent); and severity of inflammatory process (absent/mild/moderate/severe). There were no statistically significant differences among the experimental groups in new mineralized tissue formation and apical root development. The formation of vital tissue in the canal space, in turn, was statistically different between the triple paste and propolis groups: vital tissues were present in all revascularized teeth disinfected with propolis paste (100%), compared to 71% of those disinfected with the triple paste. Severity of inflammatory process was different between the triple paste and no medication groups. The new tissues formed onto canal walls and in the root canal space showed characteristics of cementum and periodontal ligament, respectively. Propolis may have some advantages over the triple paste for the revascularization of immature teeth.


Subject(s)
Animals , Dogs , Periapical Periodontitis/drug therapy , Propolis/pharmacology , Root Canal Irrigants/pharmacology , Tooth/blood supply , Neovascularization, Physiologic/drug effects , Dental Pulp Necrosis/drug therapy , Guided Tissue Regeneration/methods , Anti-Infective Agents/pharmacology , Ointments , Periapical Periodontitis/physiopathology , Periodontal Ligament/drug effects , Propolis/therapeutic use , Root Canal Irrigants/therapeutic use , Time Factors , Tooth Remineralization/methods , Random Allocation , Reproducibility of Results , Treatment Outcome , Dental Pulp Necrosis/physiopathology , Tooth Apex/drug effects , Tooth Apex/physiopathology , Dental Pulp/drug effects , Dental Pulp/physiopathology , Dental Pulp Cavity/drug effects , Dental Pulp Cavity/physiopathology , Dentin/drug effects , Anti-Infective Agents/therapeutic use
9.
Braz. j. med. biol. res ; 49(4): e4324, 2016. tab, graf
Article in English | LILACS | ID: biblio-951663

ABSTRACT

The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.


Subject(s)
Humans , Periodontal Ligament/drug effects , Interleukin-10/pharmacology , RANK Ligand/metabolism , Osteoprotegerin/metabolism , Fibroblasts/drug effects , Glucose/pharmacology , Periodontal Ligament/cytology , Time Factors , RNA, Messenger/analysis , Down-Regulation , Up-Regulation , Cells, Cultured , Blotting, Western , Analysis of Variance , Reverse Transcriptase Polymerase Chain Reaction , Fibroblasts/metabolism
10.
J. appl. oral sci ; 23(4): 383-389, July-Aug. 2015. ilus
Article in English | LILACS, BBO | ID: lil-759364

ABSTRACT

AbstractObjective RetroMTA® is a new hydraulic bioceramic indicated for pulp capping, perforations or root resorption repair, apexification and apical surgery. The aim of this study was to compare the radiopacity, pH variation and cytotoxicity of this material to ProRoot® MTA.Material and Methods Mixed cements were exposed to a digital x-ray along with an aluminum stepwedge for the radiopacity assay. pH values were verified after incubation period of 3, 24, 48, 72 and 168 hours. The cytotoxicity of each cement was tested on human periodontal ligament fibroblasts using a multiparametric assay. Data analysis was performed using ANOVA and Tukey’spost hoc in GraphPad Prism.Results ProRoot® MTA had higher radiopacity than RetroMTA®(p<0.001). No significant differences were observed for the pH of the materials throughout experimental periods (p>0.05) although pH levels of both materials reduced over time. Both ProRoot® MTA and RetroMTA® allowed for significantly higher cell viability when compared with the positive control (p<0.001). No statistical difference was observed between ProRoot® MTA and RetroMTA® cytotoxicity level in all test parameters, except for the ProRoot® MTA 48-hour extract media in the NR assay (p<0.05).Conclusion The current study provides new data about the physicochemical and biological properties of Retro® MTA concerning radiopacity, pH and cytotoxic effects on human periodontal ligaments cells. Based on our findings, RetroMTA® meets the radiopacity requirements standardized by ANSI/ADA number 572, and similar pH values and biocompatibility to ProRoot® MTA. Further studies should be performed to evaluate additional properties of this new material.


Subject(s)
Humans , Aluminum Compounds/chemistry , Calcium Compounds/chemistry , Ceramics/chemistry , Oxides/chemistry , Root Canal Filling Materials/chemistry , Silicates/chemistry , Aluminum Compounds/toxicity , Analysis of Variance , Calcium Compounds/toxicity , Cell Survival/drug effects , Cells, Cultured , Ceramics/toxicity , Contrast Media , Drug Combinations , Fibroblasts/drug effects , Materials Testing , Oxides/toxicity , Periodontal Ligament/drug effects , Reference Values , Reproducibility of Results , Root Canal Filling Materials/toxicity , Silicates/toxicity , Time Factors
11.
Braz. j. med. biol. res ; 47(12): 1036-1043, 12/2014. graf
Article in English | LILACS | ID: lil-727657

ABSTRACT

Diabetics have an increased prevalence of periodontitis, and diabetes is one of the causative factors of severe periodontitis. Apoptosis is thought to be involved in this pathogenic relationship. The aim of this study was to investigate apoptosis in human periodontal ligament (PDL) fibroblasts induced by advanced glycation end products (AGEs) and their receptor (RAGE). We examined the roles of apoptosis, AGEs, and RAGE during periodontitis in diabetes mellitus using cultured PDL fibroblasts that were treated by AGE-modified bovine serum albumin (AGE-BSA), bovine serum albumin (BSA) alone, or given no treatment (control). Microscopy and real-time quantitative PCR indicated that PDL fibroblasts treated with AGE-BSA were deformed and expressed higher levels of RAGE and caspase 3. Cell viability assays and flow cytometry indicated that AGE-BSA reduced cell viability (69.80±5.50%, P<0.01) and increased apoptosis (11.31±1.73%, P<0.05). Hoechst 33258 staining and terminal-deoxynucleotidyl transferase-mediated nick-end labeling revealed that AGE-BSA significantly increased apoptosis of PDL fibroblasts. The results showed that the changes in PDL fibroblasts induced by AGE-BSA may explain how AGE-RAGE participates in and exacerbates periodontium destruction.


Subject(s)
Animals , Cattle , Humans , Apoptosis/drug effects , Fibroblasts/drug effects , /pharmacology , Periodontal Ligament/cytology , Receptors, Immunologic/metabolism , Serum Albumin, Bovine/pharmacology , Cell Count , /metabolism , Cell Survival/drug effects , Diabetes Complications , Flow Cytometry , Fibroblasts/metabolism , Immunohistochemistry , In Situ Nick-End Labeling , Primary Cell Culture , Periodontal Diseases/complications , Periodontal Ligament/drug effects , Real-Time Polymerase Chain Reaction
12.
Dental press j. orthod. (Impr.) ; 19(2): 96-107, Mar-Apr/2014. tab, graf
Article in English | LILACS | ID: lil-714625

ABSTRACT

Introduction: Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen. Objective: The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats. Methods: Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were processed histologically. We quantified blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments. The extent of bone formation was evaluated under polarized light to determine the percentage of immature/mature collagen. Results: We observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen increased in the NM group in comparison to the CM group with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001). Conclusions: Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in developed bone matrix. .


Introdução: a nicotina apresenta efeito prejudicial sobre a angiogênese, osteogênese e síntese de colágeno. Objetivo: investigar a ação da nicotina sobre a remodelação óssea durante o movimento dentário induzido em ratos. Métodos: oitenta ratos machos Wistar foram divididos em três grupos: grupo C (sem indução de movimento dentário e sem a ação da nicotina - controle); grupo CM (indução de movimento dentário) e grupo NM (indução de movimento dentário associado à ação da nicotina). Os animais dos grupos C e CM receberam solução salina a 0,9% e os animais do grupo NM receberam nicotina (solução PA a 98% diluída em solução salina a 0,9% estéril) por via subcutânea (2mg/kg). Após a eutanásia dos animais, com 3, 7, 14 e 21 dias de uso da mola ortodôntica, os espécimes teciduais foram processados histologicamente e quantificou-se o número de vasos sanguíneos, lacunas de Howship e células osteoclásticas nos lados de tração e compressão do ligamento periodontal. A neoformação óssea foi avaliada por meio de luz polarizada, para determinar a porcentagem de colágeno maduro e imaturo. Resultados: observou-se que a quantidade de vasos sanguíneos diminuiu no grupo NM, quando comparado ao grupo CM, nos períodos de três (p < 0,001) e sete (p < 0,05) dias. Quanto às células osteoclásticas e lacunas de Howship, o grupo NM apresentou menores níveis de expressão em relação ao grupo CM, com diferença estatisticamente significativa nos períodos de 7 e 14 dias. A porcentagem de colágeno imaturo apresentou-se aumentada no grupo NM, quando comparado ao grupo CM, em todos os períodos analisados, com diferença e...


Subject(s)
Animals , Male , Rats , Alveolar Process/drug effects , Bone Remodeling/drug effects , Nicotine/pharmacology , Periodontal Ligament/drug effects , Tooth Movement Techniques , Alveolar Process/blood supply , Bone Resorption/pathology , Collagen/drug effects , Dental Alloys/chemistry , Dental Cementum/blood supply , Dental Cementum/drug effects , Molar/blood supply , Molar/drug effects , Neovascularization, Physiologic/drug effects , Nickel/chemistry , Orthodontic Wires , Osteoclasts/drug effects , Osteogenesis/drug effects , Periodontal Ligament/blood supply , Random Allocation , Rats, Wistar , Stress, Mechanical , Time Factors , Titanium/chemistry , Tooth Movement Techniques/instrumentation , Tooth Root/blood supply , Tooth Root/drug effects
13.
Braz. dent. j ; 24(5): 503-507, Sep-Oct/2013. tab, graf
Article in English | LILACS | ID: lil-697637

ABSTRACT

The aim of this study was to evaluate, by histological analysis, the effect of growth hormone (GH) on periodontal ligament and alveolar bone during experimental tooth movement in rats. Eighty male Wistar rats divided into control (C) and experimental (E) groups were examined after 3, 7, 14 and 21 days under controlled climate conditions. Orthodontic force (30 cN) was applied on the maxillary first molar by an orthodontic appliance. Group E received 0.1 IU/kg/day of GH and Group C received 0.5 mL/kg/day of saline. The samples were processed and evaluated under optical microscopy and polarized light microscopy. The Kruskal Wallis test was applied to compare the intergroup variables at 5% significance level. Group E presented a larger number of osteoclasts on the 3rd and 7th days and Howship lacunae on the 3 rd day, a smaller number of blood vessels and greater amount of mature collagen on the 3 rd and 7 th days than Group C (p<0.05). It was concluded that GH accelerated and intensified bone resorption and produced delay in immature collagen formation during experimental tooth movement.


O objetivo deste estudo foi avaliar histologicamente o efeito do hormônio de crescimento (HC) no ligamento periodontal e osso alveolar, durante a movimentação dentária induzida em ratos. Oitenta ratos Wistar, machos, divididos nos grupos controle e experimental, foram observados nos dias 3, 7, 14 e 21. Foi aplicada força ortodôntica (30 cN) sobre o primeiro molar superior por meio de um dispositivo ortodôntico. No grupo experimental foi administrada 0,1 UI/kg/dia de HC e, no grupo controle, 0,5 mL/kg/dia de solução salina. As amostras foram processadas e avaliadas por microscopia de luz e luz polarizada. O teste de Kruskal Wallis foi utilizado para a comparação das variáveis intergrupos. Verificou-se que o grupo experimental apresentou maior quantidade de osteoclastos nos 3° e 7° dias e de lacunas de Howship no 3° dia, menor quantidade de vasos sanguíneos e maior quantidade de colágeno maduro nos 3° e 7° dias do que no grupo controle (p<0,05). Concluiu-se que o HC acelerou e intensificou a reabsorção óssea e produziu atraso na formação de colágeno imaturo, durante o movimento ortodôntico induzido.


Subject(s)
Animals , Male , Rats , Alveolar Process/drug effects , Growth Hormone/pharmacology , Tooth Movement Techniques , Periodontal Ligament/drug effects , Rats, Wistar
14.
Braz. dent. j ; 24(2): 111-116, Mar-Apr/2013. tab, graf
Article in English | LILACS | ID: lil-675663

ABSTRACT

The aim of this study was to compare the in vitro cytotoxicity of white mineral trioxide aggregate (MTA), MTA Fillapex® and Portland cement (PC) on human cultured periodontal ligament fibroblasts. Periodontal ligament fibroblast culture was established and the cells were used for cytotoxic tests after the fourth passage. Cell density was set at 1.25 X10 4 cells/well in 96-well plates. Endodontic material extracts were prepared by placing sealer/cement specimens (5X3mm) in 1mL of culture medium for 72 h. The extracts were then serially two-fold diluted and inserted into the cell-seeded wells for 24, 48 and 72 h. MTT assay was employed for analysis of cell viability. Cell supernatants were tested for nitric oxide using the Griess reagent system. MTA presented cytotoxic effect in undiluted extracts at 24 and 72 h. MTA Fillapex® presented the highest cytotoxic levels with important cell viability reduction for pure extracts and at ½ and » dilutions. In this study, PC did not induce alterations in fibroblast viability. Nitric oxide was detected in extract-treated cell supernatants and also in the extracts only, suggesting presence of nitrite in the soluble content of the tested materials. In the present study, MTA Fillapex displayed the highest cytotoxic effect on periodontal ligament fibroblasts followed by white MTA and PC.


Resumo O objetivo deste estudo foi comparar a citotoxicidade in vitro de agregado trióxido mineral (MTA) branco, MTA Fillapex® e cimento Portland (PC) em cultura de fibroblastos de ligamento periodontal humano. A cultura de fibroblastos de ligamento periodontal foi estabelecida e as células foram utilizadas para os testes citotóxicos após a quarta passagem. A densidade celular foi ajustada em 1,25X10 4 células/poço em placas de 96 poços. Extratos dos materiais endodônticos foram preparados por meio da inserção de corpos de prova dos cimentos (5 X 3 mm) em 1 mL de meio de cultura durante 72 h. Os extratos foram diluídos serialmente na razão de ½ e inseridos aos poços contendo as células por 24, 48 e 72 h. Ensaio de MTT foi realizado para a avaliação da viabilidade celular. O sobrenadante das células foi testado em relação à presença de óxido nítrico utilizando o sistema de reagentes de Griess. O MTA apresentou efeito citotóxico quando o extrato era aplicado sem diluição durante 24 e 72 h. O MTA Fillapex apresentou os maiores níveis de citotoxicidade com importante redução da viabilidade celular quando o extrato foi aplicado puro e em diluições de ½ e ». Neste estudo, PC não induziu alterações na viabilidade de fibroblastos. Óxido nítrico foi detectado no sobrenadante de células tratadas com os extratos e ainda nos extratos somente, o que sugere a presença de nitrito no conteúdo solúvel dos materiais testados. No presente estudo, MTA Fillapex foi o material que demonstrou o maior efeito citotóxico sobre fibroblastos de ligamento periodontal seguido do MTA branco e do PC. .


Subject(s)
Humans , Aluminum Compounds/toxicity , Calcium Compounds/toxicity , Dental Cements/toxicity , Oxides/toxicity , Periodontal Ligament/drug effects , Root Canal Filling Materials/toxicity , Silicates/toxicity , Cell Count , Cell Culture Techniques , Cells, Cultured , Cell Survival/drug effects , Coloring Agents , Drug Combinations , Ferric Compounds/toxicity , Fibroblasts/drug effects , Materials Testing , Nitric Oxide/analysis , Nitrites/toxicity , Periodontal Ligament/cytology , Time Factors , Tetrazolium Salts , Thiazoles
15.
Article in English | IMSEAR | ID: sea-139987

ABSTRACT

Context: Today many materials have been introduced for root-end filling materials. One of them is mineral trioxide aggregate (MTA) that is mentioned as a gold standard. Aims: The purpose of this in vitro study was to evaluate the reaction of human periodontal ligament fibroblasts to the root-end filling materials, such as ProRoot MTA, Portland cement, and amalgam. Settings and Design: Eight impacted teeth were extracted in aseptic condition. The tissues around the roots were used to obtain fibroblast cells. After cell proliferation, they were cultured in the chamber slides and the extracts of the materials were added to the wells. Materials and Methods: Immunocytochemical method for measuring the expression of Fibronectin, collagen I and transforming growth factor beta (TGF®) was performed by Olysia Bioreport Imaging Software. Statistical Analysis Used: The results were analyzed by SPSS 13.0 and Tukey post hoc test with P<0.05 as the limit of significance. Results: Collagen expression in MTA specimens was higher than the other groups in 24 h significantly. After 48 h, the Portland cement group showed the most expression of collagen significantly and after 1 week, Portland cement and MTA groups had the most expression of collagen but there was no significant difference between these 2 groups. After 1 week, the Portland cement group demonstrated a higher amount of TGF® and fibronectin. Conclusions: The results suggest that Portland cement can be used as a less expensive root filling material with low toxicity. It has better effects than amalgam on the fibroblasts.


Subject(s)
Aluminum Compounds/pharmacology , Calcium Compounds/pharmacology , Cell Culture Techniques , Cells, Cultured , Collagen Type I/analysis , Collagen Type I/drug effects , Coloring Agents/diagnosis , Dental Amalgam/pharmacology , Dental Cements/pharmacology , Drug Combinations , Fibroblasts/drug effects , Fibronectins/analysis , Fibronectins/drug effects , Humans , Image Processing, Computer-Assisted , Immunohistochemistry , Materials Testing , Oxides/pharmacology , Periodontal Ligament/drug effects , Retrograde Obturation , Root Canal Filling Materials/pharmacology , Silicates/pharmacology , Time Factors , Transforming Growth Factor beta/analysis , Transforming Growth Factor beta/drug effects
16.
Braz. dent. j ; 21(1): 38-45, Jan. 2010. tab, ilus
Article in English | LILACS | ID: lil-552359

ABSTRACT

This study aimed to assess the response of apical and periapical tissues of dogs' teeth after root canal filling with different materials. Forty roots from dogs' premolars were prepared biomechanically and assigned to 4 groups filled with: Group I: commercial calcium hydroxide and polyethylene glycol-based paste (Calen®) thickened with zinc oxide; Group II: paste composed of iodoform, Rifocort® and camphorated paramonochlorophenol; Group III: zinc oxide-eugenol cement; Group IV: sterile saline. After 30 days, the samples were subjected to histological processing. The histopathological findings revealed that in Groups I and IV the apical and periapical regions exhibited normal appearance, with large number of fibers and cells and no resorption of mineralized tissues. In Group II, mild inflammatory infiltrate and mild edema were observed, with discrete fibrogenesis and bone resorption. Group III showed altered periapical region and thickened periodontal ligament with presence of inflammatory cells and edema. It may be concluded that the Calen paste thickened with zinc oxide yielded the best tissue response, being the most indicated material for root canal filling of primary teeth with pulp vitality.


O objetivo deste estudo foi avaliar a resposta dos tecidos apicais e periapicais de dentes de cães, após obturação dos canais radiculares com diferentes materiais indicados para dentes decíduos. Foram utilizados pré-molares de cães, totalizando 40 raízes que, após pulpectomia e preparo biomecânico, foram divididas em 4 grupos, nos quais os canais radiculares foram obturados com os seguintes materiais: Grupo I - pasta comercial composta de hidróxido de cálcio e polietileno glicol 400 (Calen®) espessada com óxido de zinco; Grupo II - pasta composta de iodofórmio, Rifocort® e paramonoclorofenol canforado; Grupo III - cimento de óxido de zinco e eugenol; e Grupo IV - solução salina. Decorridos 30 dias, as peças foram submetidas ao processamento histológico. De acordo com os resultados da análise histopatológica observou-se que nos Grupos I e IV as regiões apical e periapical apresentaram aspecto de normalidade, com grande número de fibras e células e ausência de reabsorção dos tecidos mineralizados. No Grupo II observou-se infiltrado inflamatório e edema leves, com discreta fibrogênese e reabsorção óssea. O Grupo III apresentou alteração na região periapical e ligamento periodontal ampliado, com presença de células inflamatórias e edema. Os resultados obtidos permitiram concluir que a pasta Calen espessada com óxido de zinco apresentou a melhor resposta tecidual, sendo a mais indicada para a obturação de canais radiculares de dentes decíduos com vitalidade pulpar.


Subject(s)
Animals , Dogs , Dental Pulp Cavity/drug effects , Root Canal Filling Materials/therapeutic use , Tooth, Deciduous/drug effects , Bicuspid/surgery , Bone Resorption/etiology , Calcium Hydroxide/therapeutic use , Camphor/therapeutic use , Chlorophenols/therapeutic use , Connective Tissue/drug effects , Drug Combinations , Dental Cementum/drug effects , Dental Disinfectants/therapeutic use , Dental Pulp Cavity/pathology , Dentin/drug effects , Edema/etiology , Hydrocarbons, Iodinated/therapeutic use , Leukocytes, Mononuclear/pathology , Materials Testing , Periapical Tissue/drug effects , Periapical Tissue/pathology , Periodontal Ligament/drug effects , Periodontal Ligament/pathology , Polyethylene Glycols/therapeutic use , Prednisolone/analogs & derivatives , Prednisolone/therapeutic use , Rifamycins/therapeutic use , Root Canal Preparation/methods , Root Resorption/etiology , Time Factors , Tooth Apex/drug effects , Tooth Apex/pathology , Tooth, Deciduous/pathology , Zinc Oxide-Eugenol Cement/therapeutic use , Zinc Oxide/therapeutic use
17.
Braz. dent. j ; 16(1): 9-16, Jan.-Apr. 2005.
Article in English | LILACS | ID: lil-415737

ABSTRACT

Foi propósito deste trabalho observar o processo de reparo de dentes de cães após obturação dos canais com dois cimentos diferentes, fazendo ou não a patência apical. Após uma sobreinstrumentação, os canais receberam um curativo de uma solução de corticosteróide-antibiótico por 7 dias, com o objetivo de obter invaginação de tecido conjuntivo para dentro dos canais. Após esse período, esse tecido foi totalmente removido em metade dos casos (grupos com patência apical) e preservados no restante dos casos (grupos sem patência apical). Os canais foram obturados pela técnica da condensação lateral empregando um cimento a base de hidróxido de cálcio (Sealer Plus) ou um cimento de Grossman (Fill Canal). Os animais foram sacrificados por overdose anestésica 60 dias após o tratamento endodôntico e as peças anatômicas foram obtidas e preparadas para análise histológica. Os dados obtidos foram analisados com base em diversos parâmetros histomorfológicos. Os resultados foram melhores nos grupos sem patência apical (p=0,01) do que nos grupos com patência. Dentre os cimentos estudados, os melhores resultados foram observados com o cimento Sealer Plus (p=0,01). Em conclusão, tanto a patência apical (presença ou ausência) quanto o tipo de material obturador de canal influíram no processo de reparo apical de dentes de cães com polpas vitais após tratamento endodôntico. O emprego de um cimento a base de hidróxido de cálcio em dentes sem patência apical promoveu os melhores resultados, dentre as condições experimentais propostas.


Subject(s)
Animals , Dogs , Root Canal Filling Materials/pharmacology , Root Canal Obturation/methods , Tooth Apex/physiology , Analysis of Variance , Barium Sulfate/pharmacology , Bismuth/pharmacology , Borates/pharmacology , Calcium Hydroxide/pharmacology , Drug Combinations , Dental Cementum/drug effects , Dental Pulp/physiology , Eugenol/pharmacology , Periodontal Ligament/drug effects , Resins, Synthetic/pharmacology , Statistics, Nonparametric , Wound Healing/drug effects , Zinc Oxide/pharmacology
18.
Bauru; s.n; 1999. 152 p. ilus, tab, graf.
Thesis in Portuguese | LILACS, BBO | ID: lil-250825

ABSTRACT

Os biofosfonatos säo eficientes bloqueadores das reabsorçöes e, por isso, säo utilizados no controle das doenças metabólicas ósseas. Os mecanismos pelos quais essas drogas interferem nos processos de reabsorçäo ainda näo estäo totalmente esclarecidos, mas incluem a inibiçäo da funçäo e a alteraçäo da morfologia dos clastos e precursores, além da atuaçäo como citotóxicos sobre os macrófagos e os osteoblastos. Num modelo experimental de movimentaçäo dentária induzida, procurou-se verificar, microscopicamente, a influência do clodronato sobre a morfologia do osso alveolar, do ligamento periodontal e do cemento dos primeiros molares superiores de 45 ratas com 90 dias de vida, nos tempos experimentais de 24 horas, 3, 5, 7, 10 e 21 dias de movimento. Os resultados microscópicos revelaram que a administraçäo do clodronato näo interferiu em nenhuma das situaçöes referidas, inclusive durante o movimento dentário induzido, nas diversas fases analisadas. A presença localizada mais intensa de infiltrado inflamatório crônico sugere uma influência dos bisfosfonatos nos processos inflamatórios


Subject(s)
Animals , Female , Infant , Rats , Clodronic Acid/adverse effects , Bone Resorption/chemically induced , Clodronic Acid/pharmacokinetics , Alveolar Process/drug effects , Alveolar Process/metabolism , Bone Diseases , Dental Cementum/drug effects , Dental Cementum/metabolism , Microscopy , Tooth Movement Techniques , Periodontal Ligament/drug effects , Periodontal Ligament/metabolism
20.
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