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1.
Rev. Ciênc. Méd. Biol. (Impr.) ; 20(4): 654-660, fev 11, 2022. fig
Article in English | LILACS | ID: biblio-1359530

ABSTRACT

Introduction: the major goal of endodontic treatment (ET) is the complete elimination and/or maximum possible reduction of bacteria and irritants in the root canal system (RCS). However, persistence of bacterial debris refractory to therapy may leading to ET failure, being necessary to realize conventional or surgical retreatment. Objective: this is a case report on the nonsurgical management of an extensive chronic periapical lesion in teeth 12 and 11 in an adult female patient with history of endodontic treatment failure. She presented with painless swelling and a fistula adjacent to tooth 11. Methodology: conventional ET combined with photodynamic therapy (PDT) was recommended, in addition to calcium hydroxide applications. Regular clinical and radiographic follow-up made over a 2-year period revealed progression of bone regeneration, without sign and symptoms, with a satisfactory outcome. Conclusion: this case report shows that the combination of conservative ET with PDT provided satisfactory results with the resolution of the chronic endodontic infection and bone repair of the extensive periapical lesion.


Introdução: o principal objetivo do tratamento endodôntico (TE) é a eliminação completa e/ou máxima redução possível de bactérias e irritantes no sistema de canais radiculares (SCR). Porém, a persistência de detritos bacterianos refratários à terapia pode levar ao insucesso do TE, sendo necessária a realização do retratamento convencional ou cirúrgico. Objetivo: este relato de caso descreve o manejo não cirúrgico de uma extensa lesão periapical crônica nos dentes 12 e 11 em paciente adulta do sexo feminino com histórico de fracasso endodôntico. A mesma queixava-se de inchaço indolor, com presença de fístula adjacente ao dente 11. Metodologia: preconizou-se a realização do TE convencional associado à terapia fotodinâmica (PDT), com três sessões de troca de hidróxido de cálcio, concluindo-se posteriormente o tratamento. Reavaliações clínicas e radiográficas periódicas ao longo de 2 anos revelaram a progressão da consolidação óssea, com um desfecho satisfatório. Conclusão: este relato de caso mostra que a combinação do TE conservador associado à PDT teve resultados satisfatórios na resolução de infecção endodôntica crônica e no reparo ósseo de lesão periapical extensa.


Subject(s)
Humans , Female , Photochemotherapy , Root Canal Therapy , Dentistry , Endodontics , Lasers
2.
Braz. j. oral sci ; 20: e214034, jan.-dez. 2021. ilus
Article in English | LILACS, BBO | ID: biblio-1282553

ABSTRACT

Aim: To compare Enterococcus faecalis reduction after antimicrobial photodynamic therapy (aPDT) used with methylene blue, toluidine blue, tannin, and curcumin as photosensitizers, an adjunct to endodontic chemomechanical preparation (CMP) in root canals of human teeth. Methods: A total of 120 single-rooted teeth were divided into 6 groups (n = 20): G1- CMP and 2.5% sodium hypochlorite (NaOCl); G2- CMP and saline solution; G3- CMP, 2.5% NaOCl, and aPDT with 0.005% methylene blue; G4- CMP, 2.5% NaOCl, and aPDT with 0.005% toluidine blue; G5- CMP, 2.5% NaOCl, and aPDT with 0.005% tannin; and G6- CMP, 2.5% NaOCl, and aPDT with 0.005% curcumin. A portable semiconductor laser was used (660 nm, 100 mW, 1.8 J, 180s) in groups 1, 2, 3, 4, 5, and a blue LED light-curing (420-480 nm, 1200 mV/cm2 ) in G6. For all groups, a 5 min pre-irradiation time was applied. Samples were collected before (initial collection), immediately after (intermediate collection) and 7 days after CMP (final collection) for colony-forming unit (CFU) counting. The Kruskal-Wallis test and analysis of variance (ANOVA) were performed (p < 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p >< 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.05; 95% confidence interval). Results: In between-group comparisons, there was no significant difference observed in the number of CFUs at the initial (p < 0.001) and final collections (p >< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies.>< 0.001) and final collections (p < 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies>< 0.001) for G2 and G3. In within-group comparisons, the number of CFUs showed a decreasing trend in G4 (p = 0.007) and G5 (p = 0.001). Conclusion: Photosensitizers promoted E. faecalis reduction, with better results for tannin and curcumin. Alternative photosensitizers should be the focus of further studies


Subject(s)
Photochemotherapy , Tannins , Photosensitizing Agents , Curcumin , Endodontics
3.
Diagn. tratamento ; 26(2): 49-57, abr.-jun. 2021. tab, ilus
Article in Portuguese | LILACS | ID: biblio-1280724

ABSTRACT

Contexto e objetivo: No mundo, aproximadamente 20 milhões de mulheres encontram-se infectadas pelo papilomavírus humano (HPV). Esta infecção pode ser assintomática ou causar papilomas verrucosos benignos, neoplasias intraepiteliais cervicais (NICs) de baixo ou alto grau, carcinoma cervical, vaginal e anal. Os tratamentos atuais para as NICs ainda são muito invasivos e destrutivos. Sendo assim, existe a necessidade do desenvolvimento de modalidades menos agressivas. A terapia fotodinâmica (TFD) atende a esses pedidos, induzindo a morte seletiva de células infectadas pelo vírus. Este artigo tem o objetivo de analisar a redução da carga viral de HPV em pacientes com lesões intraepiteliais cervicais de alto grau (LIEAG) antes e após a TFD. Métodos: Foram incluídas 28 pacientes portadoras de NICs de alto grau que realizaram tratamento com 2,5 g de creme contendo o pró-fármaco ácido 5-metil aminolevulínico (MAL) a 20%, por aproximadamente 10 horas, com posterior aplicação da luz de LED com comprimento de onda de 630 nm e irradiância de 120 mW/cm2, por 25 minutos, entregando uma dose total de 180 J/cm2, em duas sessões. As pacientes foram avaliadas por meio de captura híbrida antes e após a TFD. Resultados: Após análise criteriosa, observou-se redução significativa da carga viral dos HPVs de alta oncogenicidade após a TFD (P = 0,0334) nas pacientes com LIEAG. O mesmo não foi observado em relação aos HPVs de baixo grau oncogênico (P = 0,4038). Conclusão: A TFD parece ser eficaz e promissora na diminuição da carga viral em pacientes com LIEAG induzidos por subtipos de HPV de alto grau oncogênico.


Subject(s)
Papillomaviridae , Photochemotherapy , Cervical Intraepithelial Neoplasia , Squamous Intraepithelial Lesions , Aminolevulinic Acid , Infections
4.
Odontol. Clín.-Cient ; 20(1): 68-73, jan.-mar. 2021. ilus, tab
Article in Portuguese | LILACS, BBO | ID: biblio-1367957

ABSTRACT

A queilite actínica (QA) é uma desordem potencialmente maligna que se desenvolve principalmente no lábio inferior, decorrente da exposição crônica a luz solar. O objetivo desse estudo é elucidar, a partir de uma revisão da literatura, as abordagens não cirúrgicas mais atuais para o tratamento da QA. Foi realizada uma busca nas bases de dados PubMed, Scielo e Cochrane, sendo obtidos 280 artigos e após aplicação dos critérios de elegibilidade, foram utilizados no presente traba lho 16 estudos. Várias abordagens, como, diclofenaco sódico, mebutato de ingenol, imiquimode, 5-fluorouracil, fludroxicortida e terapia fotodinâmica, são apontadas e estudadas como forma de tratamento para a QA. Entretanto, faltam trabalhos para que se estabeleça um consenso sobre a terapêutica não-cirúrgica mais adequada... (AU)


Actinic cheilitis (AC) is a potentially malignant disorder that develops mainly on the lower lip, result ing from a chronic exposure to sunlight. The objective of this study is to elucidate, from a literature review, the most current non-surgical approaches for the treatment of AC. A search was performed in the PubMed, Scielo and Cochrane databases, obtaining 280 papers and after applying the eligibility cri teria, 16 studies were used in the present study. Various approaches, such as diclofenac sodium, ingenol mebutate, imiquimod, 5-fluorouracil, fludroxycortide and photodynamic therapy are pointed out and studied as a form of treatment for AC. However, there is a lack of work to establish a consensus on the most appropriate non-surgical therapy... (AU)


Subject(s)
Photochemotherapy , Cheilitis/drug therapy , Conservative Treatment , Lip Diseases , Sunlight , Lip
5.
São José dos Campos; s.n; 20210000. 79 p. il, graf, tab.
Thesis in Portuguese | LILACS | ID: biblio-1358938

ABSTRACT

A terapia fotodinâmica antimicrobiana (TFDa) tem sido considerada uma alternativa para o tratamento de infecções cutâneas causadas por Acinetobacter baumannii. Entretanto, é necessária a busca por fotossensibilizadores ou compostos que potencializem seus efeitos. Os objetivos desse estudo foram testar uma nova Clorina e-6 (Fotoenticine FTC) como fotossensibilizador para TFDa sobre A. baumannii, e o Farnesol como potencializador dessa terapia. Além disso, os efeitos do FTC foram comparados ao Azul de Metileno (AM), um fotossensibilizador já aprovado para uso clínico. Para isso, foram empregados os seguintes métodos: 1) Teste de concentração inibitória mínima (CIM) para estabelecer a concentração do Farnesol a ser utilizada no estudo, 2) TFDa em culturas planctônicas com ou sem tratamento prévio com Farnesol, 3) Teste de permeabilidade da membrana celular de A. baumannii e análise por microscopia confocal para avaliar a internalização dos fotossensibilizadores, 4) TFDa em biofilmes para determinar a contagem de UFC/mL de A. baumannii e a viabilidade celular por fluorescência, 5) Ensaios in vivo para avaliar os efeitos da TFDa sobre lesões de queimadura infectadas por A. baumannii em Galleria mellonella. Como resultados, observou-se no teste de CIM que o Farnesol não teve efeito antimicrobiano sobre A. baumannii. A TFDa com AM e FTC reduziu, respectivamente, 4 e 2 log (UFC/mL) de A. baumannii em crescimento planctônico. Ambos os fotossensibilizadores foram capazes de penetrar nas células bacterianas, porém o Farnesol não aumentou a permeabilidade celular. A TFDa com AM reduziu 4 log (UFC/mL) de A. baumannii em biofilmes, já a TFDa com FTC não apresentou efeito sobre a contagem de UFC/mL. A aplicação da TFDa com FTC não teve efeito sobre as lesões de queimadura em G. mellonella, entretanto a TFDa com AM aumentou a sobrevida das larvas em 35%. Concluiu-se que a TFDa com FTC teve ação antimicrobiana apenas em culturas planctônicas de A. baumannii e a ação do Farnesol associada a TFDa foi cepa dependente. Dentro dos parâmetros testados nesse estudo, a atividade antimicrobiana da TFDa com FTC foi inferior ao AM nos ensaios in vitro e in vivo


Antimicrobial photodynamic therapy (aPDT) has been an alternative for the treatment of skin infections caused by Acinetobacter baumannii. However, it is necessary to search for photosensitizers or compounds that enhance their effects. The objectives of this study were to test a new Chlorine e-6 (Fotoenticine FTC) as a photosensitizer for aPDT on A. baumannii, and Farnesol as an enhancer for this therapy. In addition the effects of FTC were compared to Methylene Blue (MB), a photosensitizer already approved for clinical use. For this, the following methods were used: 1) Minimum inhibitory concentration test (MIC) to establish the concentration of Farnesol to be used in the study, 2) aPDT in planktonic cultures with or without previous treatment with Farnesol, 3) Permeability test of the cell membrane of A. baumannii and analysis by confocal microscopy to assess the internalization of photosensitizers, 4) aPDT in biofilms to determine the CFU/mL count of A. baumannii and cell viability by fluorescence, 5) In vivo assays to evaluate the effects of aPDT on burn injuries infected by A. baumannii in Galleria mellonella. As a result, it was observed in the MIC test that Farnesol had no antimicrobial effect on A. baumannii. aPDT with MB and FTC reduced, respectively, 4 and 2 log (CFU/mL) of A. baumannii in planktonic growth. Both photosensitizers were able to penetrate bacterial cells, however Farnesol did not increase cell permeability. aPDT with MB reduced 4 log (CFU/mL) of A. baumannii in biofilms, whereas aPDT with FTC had no effect on the CFU/mL count. The application of aPDT with FTC had no effect on burn injuries in G. mellonella, however aPDT with MB increased the larvae survival by 35%. It was concluded that aPDT with FTC had antimicrobial action only in planktonic cultures of A. baumannii and the action of Farnesol associated with PDT was a dependent strain. Within the parameters tested in this study, the antimicrobial activity of aPDT with FTC was lower than MB in in vitro and in vivo assays


Subject(s)
Photochemotherapy , Burns , Photosensitizing Agents , Acinetobacter baumannii
6.
Article in Chinese | WPRIM | ID: wpr-878433

ABSTRACT

Photodynamic therapy (PDT) has developed rapidly in basic and clinical research, and its therapeutic prospects have received increasing attention. PDT has the advantages of minimally invasive, low toxicity, high selectivity, good reproducibility, protection of appearance and vital organ function, and has become a treatment. With the development of medicine, the field of application of PDT becomes more wildly, and brings a new direction for the treatment of oral diseases. This article reviews the basic principles, treatment elements and research results of PDT in the treatment of oral diseases.


Subject(s)
Humans , Mouth Diseases/drug therapy , Photochemotherapy , Photosensitizing Agents/therapeutic use , Reproducibility of Results
7.
São José dos Campos; s.n; 2021. 54 p. ilus, graf.
Thesis in Portuguese | LILACS, BBO | ID: biblio-1362071

ABSTRACT

A terapia fotodinâmica (TFD) vem se mostrando como um método eficaz no controle de micro-organismos patogênicos, sendo investigada para o tratamento de diversas doenças infecciosas, como a candidose bucal. Recentemente, alguns agentes potencializadores dessa terapia têm sido estudados, incluindo a Quitosana (QT), um polímero natural extraído do exoesqueleto de crustáceos. O objetivo desse estudo foi avaliar o efeito potencializador da QT na TFD mediada pelo fotossensibilizador Azul de Metileno (AM) sobre cepas de Candida albicans, investigando sua ação em culturas planctônicas, biofilmes e células persistentes ao fluconazol. Além disso, foi avaliado a capacidade da QT em interferir na absorção do AM pelas células de Candida. Foram utilizadas duas cepas de C. albicans, sendo uma padrão (ATCC 18804) e uma clínica isolada de candidose orofaríngea (70). Para os ensaios em culturas planctônicas, as cepas de C. albicans foram cultivadas em caldo Yeast Nitrogen Base (YNB) por 24 horas. Os biofilmes foram formados por 48 horas no fundo das placas de 96 poços. Para indução de células persistentes, C. albicans foi cultivada com altas concentrações de fluconazol por 48 horas. A seguir, foram realizados os tratamentos com QT a 5 mg/mL (pH de 6,5), AM nas concentrações de 300 ou 600 µM, e irradiação com LED (660 nm) na densidade de energia de 30 J/cm2. Foram incluídos oito grupos experimentais: TFD com AM e QT na presença de Luz (AM+QT+L+), AM e QT sem Luz (AM+QT+L-), QT e Luz (QT+L+), QT sem Luz (QT+L-), TFD com AM e Luz (AM+L+), AM sem Luz (AM+L-), Solução Fisiológica com Luz (F-L+) e apenas Solução Fisiológica (F-L-). Após os tratamentos, foi realizada contagem de unidades formadoras de colônias (UFC/mL). Para determinar a absorção do fotossensibilizador pelas células de C. albicans, as células foram lisadas e centrifugadas para leitura da densidade óptica. Os dados foram analisados por ANOVA e teste de Tukey (p<0,05%). Na TFD em culturas planctônicas, o AM (300 µm) reduziu as células de Candida em 1,6 log (UFC/mL), enquanto que a associação AM+QT levou à redução de 4,7 log. Na TFD em biofilmes, ocorreu redução microbiana de 2,9 log para o tratamento com AM (600 µm) e de 5,3 log para AM+QT. Em relação às células persistentes, a redução encontrada foi de 0,8 log para AM e 1,5 log para AM+QT. No teste de absorção, a penetração do AM nas células de Candida (DO 0,02) foi aumentada na presença da QT (DO 0,39). Concluiu-se que a QT potencializou o efeito antimicrobiano da TFD em culturas planctônicas, biofilmes e células persistentes de C. albicans, provavelmente por facilitar a penetração do AM nas células fúngicas.


Photodynamic therapy (PDT) has been shown to be an effective method to control pathogenic microorganisms, being investigated for the treatment of several infectious diseases, such as oral candidiasis. Recently, some agents that enhance this therapy have been studied, including Chitosan (CS), a natural polymer extracted from the exoskeleton of crustaceans. The aim of this study was to evaluate the potentiating effect of CS on Methylene Blue (MB) photosensitizer-mediated PDT on Candida albicans strains, investigating its action on planktonic cultures, biofilms and persister cells to fluconazole. In addition, the ability of CS to interfere with MB absorption by Candida cells was evaluated. Two strains of C. albicans were used, one standard (ATCC 18804) and one isolated clinical of oropharyngeal candidosis (70). For assays in planktonic cultures, C. albicans strains were cultivated in Yeast Nitrogen Base broth (YNB) for 24 hours. Biofilms were formed for 48 hours at the bottom of 96-well plates. For the induction of persister cells, C. albicans was cultivated with high concentrations of fluconazole for 48 hours. Next, treatments were performed with CS at 5 mg/mL (pH 6.5), MB at concentrations of 300 or 600 µM, and irradiation with LED (660 nm) at an energy density of 30 J/cm2. eight experimental groups were included: PDT with MB and CS in the presence of Light (MB+CS+L+), MB and CS without Light (MB+CS+L-), CS and Light (CS+L+), CS without Light (CS +L-), PDT with MB and Light (MB+L+), MB without Light (MB+L-), Physiological Solution with Light (F-L+) and Physiological Solution only (FL-). After the treatments, colony forming units (CFU/mL) were counted. To determine the absorption of the photosensitizer by C. albicans cells, the cells were lysed and centrifuged for optical density reading. Data were analyzed by ANOVA and Tukey test (p<0.05%). In PDT in planktonic cultures, MB (300 µm) reduced Candida cells by 1.6 log (CFU/mL), while the MB+CS association led to a 4.7 log reduction. In PDT in biofilms, there was a microbial reduction of 2.9 log for the treatment with MB (600 µm) and of 5.3 log for MB+CS. Regarding persister cells, the reduction found was 0.8 log for MB and 1.5 log for MB+CS. In the absorption test, the penetration of MB into Candida cells (OD 0.02) was increased in the presence of CS (OD 0.39). It was concluded that CS potentiated the antimicrobial effect of PDT in planktonic cultures, biofilms and persister cells of C. albicans, probably by facilitating the penetration of MB into fungal cells .


Subject(s)
Photochemotherapy , Candida albicans , Chitosan , Methylene Blue , Analysis of Variance , Photosensitizing Agents , Biofilms
8.
Braz. dent. sci ; 24(2): 1-12, 2021. tab, ilus
Article in English | LILACS, BBO | ID: biblio-1178040

ABSTRACT

Objective: The aim of this study was to evaluate the subcutaneous tissue response after different protocols to photodynamic therapy (PDT). In Phase 1, were tested the diode laser (used for 1min) associated to the photosensitizer phenothiazine chloride solution (PCS) in different concentrations. In Phase 2 ­ the diode laser and LED were tested associated to two different photosensitizers, PCS and Curcumin, in different exposure times of light application. Material and Methods: After 7, 21 and 63-days the animals were euthanized and the subcutaneous tissue processed to histological analysis. Qualitative and semi-quantitative descriptions of the inflammatory process and immunohistochemical technique were performed. The obtained data were analyzed by Kruskal-Wallis and Dunn's post-test (α= 0.5). Results: On Phase 1, the tissue response was very similar among the groups. For the inflammatory infiltrate, PCS with concentration of 10mg/mL exhibited the most intense reaction (p > 0.05). On Phase 2, at 7-days period, the analyzed parameters presented small magnitude and after 21 and 63-days, all the parameters demonstrated tissue compatibility. Conclusion: Both photosensitizers presented proper tissue compatibility regardless the different concentrations used on Phase 1 and different durations of light exposure on Phase 2 (AU)


Objetivo: Este estudo avaliou a resposta do tecido subcutâneo após terapia fotodinâmica, utilizando na Fase 1 - laser diodo por 1min e solução fotossensibilizadora de cloreto de fenotiazina (CF) em diferentes concentrações e Fase 2 - laser diodo e LED e dois fotossensibilizadores, CF e Curcumina, em diferentes tempos de exposição da aplicação de luz. Material e Métodos: Após 7, 21 e 63 dias, foram realizadas descrições qualitativas e semiquantitativas do processo inflamatório e técnica de imunoistoquímica. Os dados foram analisados pelo pós-teste de Kruskal-Wallis e Dunn (α = 0,5). Resultados: Na Fase 1, a resposta do tecido foi muito semelhante. O infiltrado inflamatório, na concentração de 10 mg / mL, exibiu reação mais intensa (p > 0,05). Na Fase 2, aos 7 dias, os parâmetros analisados apresentaram pequena magnitude. Aos 21 e 63 dias, todos os parâmetros demonstraram compatibilidade com o tecido. Conclusão: Ambos os fotossensibilizadores apresentaram compatibilidade de tecido adequada, independentemente das diferentes concentrações utilizadas na Fase 1 e diferentes durações de exposição à luz na Fase 2 (AU)


Subject(s)
Animals , Mice , Photochemotherapy , Rats, Inbred Strains , Curcumin , Subcutaneous Tissue
9.
Arq. bras. med. vet. zootec. (Online) ; 72(5): 1599-1608, Sept.-Oct. 2020. tab, graf, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1131555

ABSTRACT

A doença periodontal é uma afecção comum, relacionada ao aprisionamento de alimentos em diastemas não fisiológicos, em equinos. O tratamento consiste na correção da causa primária, limpeza e desbridamento do sulco gengival, denominado tratamento convencional (TC). Frequentemente antimicrobianos são necessários, pela gravidade ou patogenicidade dos agentes. A terapia fotodinâmica adjuvante (TF) tem sido estudada pelo seu potencial combate bacteriano, sem causar resistência bacteriana. O objetivo deste estudo foi analisar o uso da TF na doença periodontal, experimentalmente induzida, em dentes incisivos de equinos, e compará-la com o TC. O TC não resultou em melhora clínica estatisticamente, tanto em graus como em profundidade, apenas numérica na profundidade aos 30 dias. A TF foi empregada em dentes com profundidade maior da bolsa periodontal que a do grupo TC e, mesmo assim, apresentou melhora clínica já com sete dias, e mais efetiva aos 30, atingindo, em média, o valor considerado normal, três milímetros. A TF apresentou potencial para ser aplicada na rotina, pelo incremento nos resultados, sem causar efeitos colaterais.(AU)


Periodontal disease is a common condition, related to the entrapment of food in non-physiological diastemas in horses. Treatment consists of correction of the primary cause, cleansing and debridement of the gingival sulcus, called Conventional Therapy (CT). Often antimicrobials are requested due to the severity or pathogenicity of the agents. Adjuvant Photodynamic Therapy (PDT), has been studied for its potential bacterial combat, without causing bacterial resistance. The objective of this study was to analyze the use PDT with the experimentally induced periodontal disease in the incisor teeth of horses, and to compare with the CT. The CT did not result in clinical improvement, either in degrees or millimeters. The PDT was used in teeth with a greater depth of the periodontal pocket than the TC group, and even then, showed clinical improvement in only seven days, and more effective at 30, reaching the three millimeter value considered normal on average. The PDT presented the potential to be applied in the routine by the increase in the results without causing side effects.(AU)


Subject(s)
Animals , Periodontal Diseases/therapy , Periodontitis/veterinary , Diastema/therapy , Horses , Photochemotherapy/veterinary
10.
Braz. j. biol ; 80(3): 497-505, July-Sept. 2020. graf
Article in English | LILACS | ID: biblio-1132403

ABSTRACT

Abstract Little is known regarding whether photodynamic therapy (PDT)-induced cell death can substantially compromise macrophages (MΦ), which are important cells in PDT-induced immune responses. Here, parameters of PDT-mediated MΦ cytotoxicity and cytokine production in response to protoporphyrin IX (PpIX) were evaluated. Peritoneal MΦ from BALB/c mice were stimulated in vitro with PDT, light, PpIX, or lipopolysaccharide (LPS). After that, cell viability, lipid peroxidation, Nitric Oxide (NO), DNA damage, TNF-α, IL-6 and IL-10 were evaluated. Short PDT exposure reduced cell viability by 10-30%. There was a two-fold increase in NO and DNA degradation, despite the non-increase in lipoperoxidation. PDT increased TNF-α and IL-10, particularly in the presence of LPS, and decreased the production of IL-6 to 10-fold. PDT causes cellular stress, induces NO radicals and leads to DNA degradation, generating a cytotoxic microenvironment. Furthermore, PDT modulates pro- and anti-inflammatory cytokines in MΦ.


Resumo Pouco se sabe se a morte celular induzida pela terapia fotodinâmica (PDT) compromete os macrófagos (MΦ), envolvidos nas respostas imunes induzidas pela PDT. Neste estudo, foram avaliados parâmetros de citotoxicidade dos MΦ mediada pela PDT e a produção de citocinas, frente à protoporfirina IX (PpIX). MΦ peritoneais de camundongos BALB/c foram estimulados in vitro com PDT, luz, PpIX ou lipopolissacarídeo (LPS). Após isto, a viabilidade celular (VC), a lipoperoxidação, os níveis de óxido nítrico (NO), de DNA degradado, de TNF-α, IL-6 e IL-10 foram avaliados. A exposição curta à PDT reduziu a VC em 10-30%. Os níveis de NO e de DNA degradado duplicaram, sem aumento da lipoperoxidação. Houve aumento de TNF-α e IL-10, sendo maior na presença de LPS. Já a produção de IL-6 reduziu em dez vezes. A PDT induz estresse celular, gera radicais NO e causa dano ao DNA, tornando o microambiente citotóxico. Ainda, modula citocinas pró e anti-inflamatórias em MΦ.


Subject(s)
Animals , Rabbits , Photochemotherapy , Interleukin-10 , Protoporphyrins , Cytokines , Interleukin-6 , Tumor Necrosis Factor-alpha , Macrophages , Mice, Inbred BALB C
11.
An. bras. dermatol ; 95(4): 529-531, July-Aug. 2020. tab
Article in English | ColecionaSUS, LILACS, ColecionaSUS | ID: biblio-1130898
12.
Arq. bras. oftalmol ; 83(4): 277-282, July-Aug. 2020. tab
Article in English | LILACS | ID: biblio-1131614

ABSTRACT

ABSTRACT Purpose: This study was performed to evaluate the outcomes of accelerated corneal cross-linking in keratoconic corneas with thinnest pachymetry values of <400 µm. Methods: The study included 28 eyes of 24 patients. The uncorrected and best-corrected visual acuities (logMAR), flattest and steepest keratometric readings, central corneal thickness at the thinnest point, corneal higher-order aberrations, and contrast sensitivity were assessed before and at 1, 3, 6, 12, and 24 months after corneal cross-linking. Result: The mean best-corrected visual acuity and contrast sensitivity increased (p=0.02, p=0.03, respectively), whereas the mean uncorrected visual acuity did not significantly differ (p>0.05) at 24 months after corneal cross-linking, compared with measurements before corneal cross-linking. Although the mean flattest keratometric reading showed no significant change (p=0.58), the mean steepest keratometric reading was reduced when compared with its value before corneal cross-linking (p=0.001). No change was observed in the mean central corneal thickness at the thinnest point at 24 months after corneal cross-linking, compared with its value before corneal cross-linking (p=0.12). Conclusion: Accelerated corneal cross-linking in keratoconic eyes with thin corneas could halt the progression of keratoconus in corneas thinner than 400 µm at 24 months after treatment.


RESUMO Objetivo: Este estudo foi realizado para avaliar os resultados do cross-linking corneano acelerado em córneas ceratocônicas com os valores mais baixos de paquimetria <400 µm. Métodos: O estudo incluiu 28 olhos de 24 pacientes. As acuidades visuais não corrigidas e melhor corrigidas (logMAR), leituras ceratométricas mais planas e íngremes, espessura corneana central no ponto mais fino, aberrações corneanas de mais alta ordem e a sensibilidade ao contraste foram avaliadas antes e em 1, 3, 6, 12 e 24 meses após a realização do do cross-linking. Resultados: A média da acuidade visual melhor corrigida e a sensibilidade ao contraste aumentaram (p=0,02, p=0,03, respectivamente), enquanto a média da acuidade visual não corrigida não diferiu significativamente (p>0,05) aos 24 meses após o cross-linking, comparada com medidas antes do procedimento. Embora a leitura da média da ceratometria mais plana não tenha apresentado alteração significativa (p=0,58), a leitura ceratométrica mais íngreme diminuiu quando comparada ao seu valor antes do cross-linking (p=0,001). Não foi observada alteração na média da espessura corneana central no ponto mais fino aos 24 meses após o cross-linking em comparação com seu valor antes do procedimento (p=0,12). Conclusão: O cross-linking corneano acelerado nos olhos ceratocônicos com córneas finas pode interromper a progressão do ceratocone nas córneas mais finas que 400 µm 24 meses após o tratamento.


Subject(s)
Humans , Photochemotherapy , Collagen/therapeutic use , Cornea , Corneal Topography , Cross-Linking Reagents/therapeutic use , Riboflavin/therapeutic use , Ultraviolet Rays , Follow-Up Studies , Photosensitizing Agents/therapeutic use , Keratoconus/drug therapy
13.
Int. j. odontostomatol. (Print) ; 14(1): 12-18, mar. 2020. tab, graf
Article in Spanish | LILACS | ID: biblio-1056495

ABSTRACT

RESUMEN: La caries dental no tratada es la enfermedad crónica más frecuente en adultos y la caries no tratada en dientes temporales es la décima enfermedad crónica más frecuente. En Chile los adultos presentan un COPD promedio entre 3,76 - 22,16, pero los estudios no son en población representativa y no existen estudios comparativos entre ceod/ COPD e ICDASTM. A nivel mundial, su prevalencia puede alcanzar el 100 % de la población estudiada. Se buscará evaluar si existen diferencias en la sensibilidad y especificidad en la detección de caries utilizando ICDASTM y ceod/COPD. Se realizó una revisión sistemática cualitativa realizando una búsqueda sobre los índices de caries ceod/COPD y sistema ICDASTM, tanto en Chile como a nivel mundial, en las bases de datos Medline, ClinicalKey y SciELO encontrando 3.581, de ellos, 21 se leyeron por completo y 14 cumplieron nuestros criterios. La sensibilidad y especificidad es mayor con ICDASTM que con el índice ceod/COPD y entrega hasta un 43 % más información al detectar lesiones no cavitadas, pero necesita de mayor tiempo para su realización y más recursos al utilizar luz, aire comprimido y una profilaxis previa al examen. Capacitaciones previas, conocimientos y experiencia en ICDASTM son fundamentales para el mejor resultado, estudios demostraron que mientras mayor experiencia, la sensibilidad y especificidad aumentaba a diferencia del índice ceod/COPD. Se deben realizar más estudios comparativos entre ambos índices e incluir ICDASTM en niños por su capacidad de detectar lesiones no cavitadas las cuales son las más prevalentes en dientes temporales.


ABSTRACT: Untreated dental caries is the most frequent chronic disease in adults and untreated caries in primary teeth is the tenth most frequent chronic disease. In Chile, adults have an average DMFT between 3.76-22.16, but the studies are not in a representative population and there are no comparative studies between dmft/DMFT and ICDASTM. Worldwide, its prevalence can reach 100 % of the population studied. The objective of the study was to evaluate if there are differences in the sensitivity and specificity in the detection of caries using ICDASTM and dmft/DMFT. A qualitative systematic review was done, carrying out a search on the dmft/DMFT index and the ICDASTM system, both in Chile and worldwide, in the Medline, ClinicalKey and SciELO databases, finding 3,581, of which, 21 were read completely and 14 met the inclusion and exclusion criteria. The sensitivity and specificity is greater with ICDASTM than with the dmft/DMFT index and delivers up to 43 % more information when detecting non-cavitated lesions, but it needs more time for its realization and more resources because it needs to use light, compressed air and prophylaxis before the examination. Previous training, knowledge and experience in ICDASTM are fundamental for the best results. Studies showed that the greater the experience, the sensitivity and specificity increased, unlike the dmft/DMFT index. More comparative studies should be conducted between both indexes and include ICDASTM in children for their ability to detect non-cavitated lesions, which are the most prevalent in temporary teeth.


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Photochemotherapy/methods , DMF Index , Dental Caries/diagnostic imaging , Time Factors , Photosensitizing Agents , Dental Caries Susceptibility
14.
Braz. j. med. biol. res ; 53(1): e8389, Jan. 2020. tab, graf
Article in English | LILACS | ID: biblio-1055479

ABSTRACT

Photodynamic therapy (PDT) promotes cell death, and it has been successfully employed as a treatment resource for neuropathic complications of diabetes mellitus (T1DM) and hepatocellular carcinoma. The liver is the major organ involved in the regulation of energy homeostasis, and in pathological conditions such as T1DM, changes in liver metabolic pathways result in hyperglycemia, which is associated with multiple organic dysfunctions. In this context, it has been suggested that chlorophyll-a and its derivatives have anti-diabetic actions, such as reducing hyperglycemia, hyperinsulinemia, and hypertriglyceridemia, but these effects have not yet been proven. Thus, the biological action of PDT with chlorophyll-a on hepatic parameters related to energy metabolism and oxidative stress in T1DM Wistar rats was investigated. Evaluation of the acute effects of this pigment was performed by incubation of isolated hepatocytes with chlorophyll-a and the chronic effects were evaluated by oral treatment with chlorophyll-based extract, with post-analysis of the intact liver by in situ perfusion. In both experimental protocols, chlorophyll-a decreased hepatic glucose release and glycogenolysis rate and stimulated the glycolytic pathway in DM/PDT. In addition, there was a reduction in hepatic oxidative stress, noticeable by decreased lipoperoxidation, reactive oxygen species, and carbonylated proteins in livers of chlorophyll-treated T1DM rats. These are indicators of the potential capacity of chlorophyll-a in improving the status of the diabetic liver.


Subject(s)
Animals , Male , Rats , Chlorophyll/analogs & derivatives , Photosensitizing Agents/administration & dosage , Oxidative Stress/drug effects , Diabetes Mellitus, Experimental/drug therapy , Glycolysis/drug effects , Liver/physiopathology , Photochemotherapy , Chlorophyll/administration & dosage , Rats, Wistar , Oxidative Stress/physiology , Diabetes Mellitus, Experimental/pathology , Drug Therapy, Combination , Energy Metabolism/drug effects , Glycolysis/physiology , Liver/pathology
15.
Rev. bras. odontol ; 77(1): 1-6, jan. 2020. ilus, tab
Article in English | LILACS | ID: biblio-1103876

ABSTRACT

O objetivo do presente estudo foi avaliar a eficácia clínica e histopatológica da terapia fotodinâmica (TFD) associada à terapia a laser de baixa potência no tratamento de pacientes com queilite actínica (QA) após seis meses de acompanhamento. Material e Métodos: pacientes com QA ativa foram submetidos a duas sessões de TFD associadas à laserterapia de baixa potência, utilizando o 5-aminolevulinato de metila como droga fotossensibilizadora. Após seis meses de acompanhamento, uma nova biópsia incisional foi realizada para avaliar a resposta ao tratamento. Resultados: excelentes taxas de eficácia foram observadas na melhora dos sintomas neste estudo, no entanto, a análise histopatológica não foi tão promissora e apenas a melhora do grau de displasia só pode ser observada em 20% da amostra. Conclusão: de acordo com os resultados do presente estudo, pode-se concluir que a terapia fotodinâmica associada à laserterapia de baixa potência parece ser uma opção a ser considerada no tratamento da Queilite Actínica. No entanto, os pacientes devem ser acompanhados de perto clinicamente e, eventualmente, histopatologicamente


Objective: the aim of the present study was to evaluate the clinical and histopathological efficacy of photodynamic therapy (PDT) associated with the low-level laser therapy (LLLT) in the treatment of patients with Actinic Cheilitis (AC) after a six-month follow-up. Material and Methods: patients with active AC undergone through two sessions of PDT, associated with the low-level laser therapy using the Methyl 5-aminolevulinate as a photosensybilizer drug. After a six-month follow-up, a new incisional biopsy was performed in order to evaluate the response to treatment. Results: excellent efficacy rates were observed in the improvement of symptoms in this study, however, histopathological analysis was not so promising and the improvement of dysplasia degree could only be seen in 20% of the sample. Conclusion: according to the results of the present study, we concluded that the photodynamic therapy associated with low-level laser therapy is an option to be considered when treating Actinic Cheilits. However, patients should be clinically followed-up in a severing agenda and eventually, histopathologically


Subject(s)
Photochemotherapy , Low-Level Light Therapy , Cheilitis/therapy
16.
Article in Chinese | WPRIM | ID: wpr-878386

ABSTRACT

OBJECTIVE@#To compare the anti-caries effect and safety of Er:YAG laser combined with fluoride and methylene blue-photodynamic therapy (MB-PDT).@*METHODS@#A total of 28 rat dental caries models were established and randomly divided into seven groups: photodynamic therapy (PDT) group, laser combined with fluoride group, laser group, sodium fluoride group, and 0.9% saline control group. Spectrophotometric optical density was used to reflect the growth of Streptococcus mutans. Laser-induced fluorescence diagnostic (LF) instrument was utilized to detect the demineralization degree of dental caries. Histopathological sections were employed to observe the damage of dental pulp and buccal mucosa.@*RESULTS@#The optical density (OD) value of the PDT and combination groups was significantly lower than that of other treatment groups (P<0.05). An increase in LF value and demineralization occurred in varying degrees with different treatment methods. Histopathological observation showed that pulp and buccal mucosa injury was more obvious in the combination group of 70 mw·cm⁻² and Er:YAG laser group compared with other groups.@*CONCLUSIONS@#Under the same parameters, the combined group and PDT have good germicidal efficacy, but PDT has fewer adverse reactions and less damage. It is an effective and safe method for caries prevention.


Subject(s)
Cariostatic Agents , Dental Caries/prevention & control , Fluorides , Humans , Laser Therapy , Lasers, Solid-State , Methylene Blue , Photochemotherapy
18.
Article in Chinese | WPRIM | ID: wpr-826367

ABSTRACT

To investigate the effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on () biofilm. biofilms were constructed on a cell slide and treated with ALA-PDT.According to different light doses,the biofilms were divided into six groups:ALA-PDT group [ALA-PDT1 (50 J/cm),ALA-PDT2 group (100 J/cm),ALA-PDT3 group (200 J/cm)],ALA-only group (ALA group),light-only group (LED),and a negative control group (ALA-PDT-group).The biofilm structure and the ratio of the dead bacteria/live bacteria were observed using a laser confocal microscope (CLSM).Biofilm viability was measured using the XTT assay. CLSM showed that the biofilm structures of ALA group and LED group were not significantly different from that of ALA-PDT-group,whereas the biofilm structure was more seriously damaged in ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group than in the ALA-PDT-group.The ratios of the dead/live bacteria in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group were 0.350±0.033, 0.305±0.046, 0.330±0.032, 1.525±0.439, 2.293±0.148 and 3.092±0.189,respectively.ALA group(=0.003, =1.000)and LED group(=-0.025, =1.000)did not significantly differ from the ALA-PDT-group.However,the ratio of dead/live bacteria in ALA-PDT-group was significantly lower than those in ALA-PDT1 group (=-0.162, <0.001),ALA-PDT2 group (=-0.254, <0.001),and ALA-PDT3 group (=-0.352, <0.001).The values of the XTT assay were were 0.462±0.028,0.465±0.044,0.437±0.047,0.301±0.040,0.207±0.001,and 0.110±0.007,respectively,in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group.Although the values of XTT assay in ALA(=-0.044, =1.000)and LED groups (=-0.020, =1.000)did not significantly differ from that in ALA-PDT-group,it was significantly higher in ALA-PDT-group than in ALA-PDT1 group (=1.175, <0.001),ALA-PDT2 group (=1.942, <0.001),and ALA-PDT3 group (=-0.352, =2.742, <0.001). ALA-PDT has an inhibitory effect on biofilm.ALA-PDT destroys biofilm structure and inhibits biofilm viability.


Subject(s)
Aminolevulinic Acid , Biofilms , Photochemotherapy , Photosensitizing Agents , Propionibacterium acnes
19.
Braz. j. med. biol. res ; 53(5): e8457, 2020. tab, graf
Article in English | LILACS | ID: biblio-1098118

ABSTRACT

The objective of this study was to investigate whether the conjugation of gold nanoparticles (GNPs) to 5-aminolevulinic acid (5-ALA) could enhance the anti-tumor efficiency of photodynamic therapy (PDT) in epidermoid carcinoma cells. The mRNA and protein expression levels were determined by quantitative real-time PCR and western blot, respectively. Cell viability, apoptosis, invasion, and migration were determined by MTT assay, flow cytometry, transwell invasion assay, and migration assay, respectively. Singlet oxygen generation was detected by the singlet oxygen sensor green reagent assay. Our results showed that PDT with 5-ALA and GNPs-conjugated 5-ALA (5-ALA-GNPs) significantly suppressed cell viability, increased cell apoptosis and singlet oxygen generation in both HaCat and A431 cells, and PDT with 5-ALA and 5-ALA-GNPs had more profound effects in A431 cells than that in HaCat cells. More importantly, 5-ALA-GNPs treatment potentiated the effects of PDT on cell viability, cell apoptosis, and singlet oxygen generation in A431 cells compared to 5-ALA treatment. Further in vitro assays showed that PDT with 5-ALA-GNPs significantly decreased expression of STAT3 and Bcl-2 and increased expression of Bax in A431 cells compared with PDT with 5-ALA. In addition, 5-ALA-GNPs treatment enhanced the inhibitory effects of PDT on cell invasion and migration and Wnt/β-catenin signaling activities in A431 cells compared to 5-ALA treatment. In conclusion, our results suggested that GNPs conjugated to 5-ALA significantly enhanced the anti-tumor efficacy of PDT in A431 cells, which may represent a better strategy to improve the outcomes of patients with cutaneous squamous cell carcinoma.


Subject(s)
Humans , Skin Neoplasms/pathology , Carcinoma, Squamous Cell/pathology , Metal Nanoparticles/administration & dosage , Levulinic Acids/pharmacology , Photochemotherapy , RNA, Neoplasm , Cell Survival/drug effects , Apoptosis/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects
20.
Rev. Pesqui. (Univ. Fed. Estado Rio J., Online) ; 12: 505-509, jan.-dez. 2020. tab
Article in English, Portuguese | LILACS, BDENF | ID: biblio-1097201

ABSTRACT

Objetivo: avaliar a eficácia antimicrobiana da terapia fotodinâmica no tratamento da candidíase oral em pessoas vivendo com HIV/aids. Método: estudo experimental, qualitativo e descritivo com 18 pessoas vivendo com HIV/aids que manifestavam a candidíase oral, maiores de 18 anos, que estavam em tratamento no Hospital Universitário Gaffrée e Guinle. Este grupo subdividiu-se em um grupo controle, composto por sete pessoas, que recebeu tratamento com a terapia fotodinâmica e antifúngicos, e um grupo experimental, com 11, que recebeu apenas a terapia fotodinâmica. A evolução do tratamento de cada participante foi acompanhada por registros fotográficos em duas consultas, inicial e final. Esta pesquisa foi aprovada pelo Comitê de Ética em Pesquisa do hospital, parecer número 2.431.107. Resultados: a maioria dos participantes apresentou melhora clínica, ainda que discreta, e em apenas um houve piora clínica. Conclusão: a terapia fotodinâmica antimicrobiana pode ser eficaz no tratamento da candidíase oral em pessoas vivendo com HIV/Aids


Objective: evaluate the antimicrobial efficacy of photodynamic therapy in the treatment of oral candidiasis in people living with HIV/ aids. Method: experimental, qualitative and descriptive study with 18 people living with HIV/aids who presented oral candidiasis, over 18 years of age, who were being treated at the Gaffrée and Guinle University Hospital. This group was subdivided into a control group, composed of seven people, who received treatment with photodynamic and antifungal therapy, and an experimental group, with 11, who received only the photodynamic therapy. The evolution of the treatment of each participant was followed by photographic registers in two appointments, initial and final. This research was approved by the Research Ethics Committee from the hospital, dictum number 2.431.107. Results: most of the participants showed clinical improvement, albeit discrete, and in only one there was clinical worsening. Conclusion: antimicrobial photodynamic therapy may be effective in the treatment of oral candidiasis in people living with HIV/Aids


Objetivo: evaluar la eficacia antimicrobiana de la terapia fotodinámica en el tratamiento de la candidiasis bucal en personas que viven con VIH/sida. Método: estudio experimental, cualitativo y descriptivo con 18 personas viviendo con VIH/sida que manifestaban la candidiasis bucal, mayores de 18 años, que estaban en tratamiento en el Hospital Universitario Gaffrée y Guinle. Este grupo se subdividió en grupo control, compuesto por siete personas, que recibió tratamiento con la terapia fotodinámica y antifúngicos, y un grupo experimental, con 11, que recibió sólo la terapia fotodinámica. La evolución del tratamiento de cada participante fue acompañada por registros fotográficos en dos consultas, inicial y final. La investigación fue aprobada por el Comité de Ética en Investigación del lugar, dictamen número 2.431.107. Resultados: la mayoría de los participantes presentó mejoría clínica, aunque discreta, y en apenas uno hubo empeoramiento clínico. Conclusión: la terapia fotodinámica antimicrobiana puede ser eficaz en el tratamiento de la candidiasis bucal en personas que viven con el VIH/Sida


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Aged, 80 and over , Photochemotherapy/statistics & numerical data , Candidiasis, Oral/therapy , AIDS-Related Opportunistic Infections/therapy , Photochemotherapy/methods , Candidiasis, Oral/epidemiology , Acquired Immunodeficiency Syndrome/therapy , HIV , Drug Resistance, Fungal
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