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ABCD arq. bras. cir. dig ; 33(2): e1533, 2020. tab, graf
Article in English | LILACS | ID: biblio-1130522


ABSTRACT Background: Despite all the advances in medicine and the wide variety of dressings available, the treatment of burn wounds still represents an important medical challenge. The pinus cellulose membrane dressing is a biomaterial with characteristics similar to those of bacterial cellulose, but with lower cost. Aim: To evaluate the efficacy of pinus nanocellulose membrane on healing of deep second degree burns in rats and compare with Membracel®. Method: Thirty male Wistar rats were submitted to deep second degree burn in dorse, with boiling water at 97o C for 20 s, generating a 314 mm² area wound. The animals were distributed in three dressing groups (n=10): group 1 - simple gauze; group 2 - bacterial cellulose membrane (Membracel®); and group 3 - pinus cellulose membrane. They were evaluated for 20 days to verify clinical condition, macro and microscopic appearance and wound contraction. Results: All of them remained clinically well with no differences in weight. Crusts were observed in group 1, and none in groups 2 and 3. Regarding to scar contraction, groups 2 and 3 were similar, better than group 1. Microscopic analysis showed predominance of advanced healing degree in groups 1 and 3, and initial in group 2. Mature collagen was predominant in all groups. Conclusion: The pinus nanocellulose membrane is effective in the treatment of experimental second degree burn in rats and its effectiveness is similar to that of the bacterial nanocellular membrane.

RESUMO Racional: Apesar de todos os avanços da medicina e da grande variedade de curativos disponíveis, o tratamento das queimaduras ainda representa importante desafio médico. O curativo de membrana de celulose de pinus é biomaterial com características semelhantes à de celulose bacteriana, mas de menor custo. Objetivo: Avaliar a eficácia da membrana de nanocelulose de pinus na cicatrização de queimaduras profundas de segundo grau em ratos e comparar com a Membracel®. Método: Trinta ratos Wistar machos foram submetidos à queimadura profunda de segundo grau em dorso, com água fervente a 97o C por 20 s, gerando lesão de 314 mm². Os animais foram distribuídos em três grupos de curativos (n = 10): grupo 1 - gaze simples; grupo 2 - membrana de celulose bacteriana (Membracel®); e grupo 3 - membrana de celulose de pinus. Eles foram avaliados por 20 dias para verificar o quadro clínico, aspecto macro e microscópico e a contração da ferida. Resultados: Todos permaneceram clinicamente bem, sem diferenças de peso. Crostas foram observadas no grupo 1 e nenhuma nos grupos 2 e 3. Em relação à contração da cicatriz, os grupos 2 e 3 foram semelhantes, melhores que o grupo 1. A análise microscópica mostrou predomínio de grau de cicatrização avançado nos grupos 1 e 3, e inicial no grupo 2. O colágeno maduro foi predominante em todos os grupos. Conclusão: A membrana de nanocelulose de pinus é eficaz no tratamento de queimaduras experimentais de segundo grau em ratos e sua eficácia é semelhante à da membrana nanocelular bacteriana.

Animals , Male , Rats , Bandages , Wound Healing , Burns/therapy , Cellulose/pharmacology , Pinus/chemistry , Collagen , Rats, Wistar
Electron. j. biotechnol ; 40: 40-44, July. 2019. tab, ilus
Article in English | LILACS | ID: biblio-1053231


Background: The study of plant-associated microorganisms is very important in the discovery and development of bioactive compounds. Pseudomonas is a diverse genus of Gammaproteobacteria comprising more than 60 species capable of establishing themselves in many habitats, which include leaves and stems of many plants. There are reports of metabolites with diverse biological activity obtained from bacteria of this genus, and some of the metabolites have shown cytotoxic activity against cancer cell lines. Because of the high incidence of cancer, research in recent years has focused on obtaining new sources of active compounds that exhibit interesting pharmacodynamic and pharmacokinetic properties that lead to the development of new therapeutic agents. Results: A bacterial strain was isolated from tumors located in the stem of Pinus patula, and it was identified as Pseudomonas cedrina. Extracts from biomass and broth of P. cedrina were obtained with chloroform:methanol (1:1). Only biomass extracts exhibited antiproliferative activity against human tumor cell lines of cervix (HeLa), lung (A-549), and breast (HBL-100). In addition, a biomass extract from P. cedrina was fractioned by silica gel column chromatography and two diketopiperazines were isolated: cyclo-(L-Prolyl-L-Valine) and cyclo-(L-Leucyl-L-Proline). Conclusions: This is the first report on the association of P. cedrina with the stems of P. patula in Mexico and the antiproliferative activity of extracts from this species of bacteria against human solid tumor cell lines.

Pseudomonas/chemistry , Pinus/microbiology , Cell Line, Tumor/drug effects , Antineoplastic Agents/pharmacology , Plants/microbiology , Symbiosis , Biomass , Gammaproteobacteria/chemistry , Cell Proliferation/drug effects
Rev. Soc. Cardiol. Estado de Säo Paulo ; 29(2 (Supl)): 197-200, abr.-jun. 2019. ilus, tab
Article in English, Portuguese | LILACS | ID: biblio-1009734


A doença aterosclerótica crônica (DAC) é uma condição bastante prevalente em nosso meio e uma das principais doenças cardiovasculares ligadas ao envelhecimento. Dentre as opções terapêuticas adjuvantes, o Picnogenol ®, extrato da casca do Pinus pinaster, tem sido alvo de estudo em decorrência de função antioxidante, anti-inflamatória e antiplaquetária. Este artigo é uma revisão narrativa, cujo objetivo é avaliar o uso do Picnogenol® como opção terapêutica da DAC. Os estudos incluídos foram pesquisados nas bases de dados: PubMed, Scielo, The Cochrane Library, Scopus e LILACS, sendo excluídos, considerando suas restrições para avaliação terapêutica, os relatos de caso e séries de caso com n ≤ 5. Como resultado, os estudos têm apontado vantagens do uso Picnogenol® no tratamento da DAC, assim como de outras doenças cardiovasculares, porém, o número de pesquisas ainda é pequeno (principalmente ensaios clínicos) e há importantes limitações de tamanho amostral, o que dificulta sua atual recomendação na prática clínica

Chronic atherosclerosis is a highly prevalent condition and one of the main cardiovascular diseases linked to the aging process. Among the adjuvant therapeutic options, Pycnogenol® (Pinus pinaster bark extract) has been studied because of its antioxidant, anti-inflammatory and antiplatelet functions. This article is a narrative review aimed at evaluating the use of Pycnogenol® as a therapeutic option in the treatment of chronic atherosclerosis. The studies included were obtained from the following databases: PubMed, Scielo, The Cochrane Library, Scopus and LILACS. Case reports and case series with n≤5 were excluded due to their restrictions for therapeutic evaluation. As a result, the studies have indicated advantages in the use of Pycnogenol® in the treatment of chronic atherosclerosis as well as other cardiovascular diseases. However, the number of studies is still small (particularly clinical trials), and there are important sample size limitations, which restricts its current recommendation in clinical practice

Humans , Male , Female , Pinus , Atherosclerosis/physiopathology , Aging , Cardiovascular Diseases/mortality , Chronic Disease , Risk Factors , Diabetes Mellitus , Heart Diseases , Hypertension , Phytotherapy/methods , Antioxidants
Experimental Neurobiology ; : 593-601, 2019.
Article in English | WPRIM | ID: wpr-763787


3-Carene, a bicyclic monoterpene, is one of the major components of the pine tree essential oils. It has been reported that, in addition to its known properties as a phytoncide, 3-carene has anti-inflammatory, antimicrobial, and anxiolytic effects. We have previously demonstrated that α-pinene, the major component of pine tree, has a hypnotic effect through GABA(A)-benzodiazepine (BZD) receptors. However, a hypnotic effect of 3-carene has not been studied yet. Here, we report that oral administration of 3-carene increases the sleep duration and reduces sleep latency in pentobarbital-induced sleep test. 3-Carene potentiates the GABA(A) receptor-mediated synaptic responses by prolonging the decay time constant of inhibitory synaptic responses. These enhancing effects of 3-carene are reproduced by zolpidem, a modulator for GABA(A)-BZD receptor, and fully inhibited by flumazenil, an antagonist for GABA(A)-BZD receptor. The molecular docking of 3-carene to the BZD site of GABA(A) protein structure, suggests that 3-carene binds to the BZD site of α1 and ϒ2 subunits of GABA(A)-BZD receptor. These results indicate that, similar to α-pinene, 3-carene shows a sleep-enhancing effect by acting as a positive modulator for GABA(A)-BZD receptor.

Administration, Oral , Anti-Anxiety Agents , Flumazenil , Hypnotics and Sedatives , Oils, Volatile , Pinus
Mycobiology ; : 31-39, 2019.
Article in English | WPRIM | ID: wpr-760529


Three fungal isolates designated as CNUFC-YR329-1, CNUFC-PTS103-1, and CNUFC-PTS2-1 were discovered during a survey of fungal diversity of the order Mortierellales from freshwater and pine tree rhizosphere soil samples in Korea. The strains were analyzed morphologically and phylogenetically based on the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA gene sequences. Based on their morphology and phylogeny, the three isolates were identified as Mortierella elongata, M. horticola, and M. humilis, respectively. To the best of our knowledge, M. elongata, M. horticola, and M. humilis, belonging to an undiscovered taxon, have not been previously described in Korea.

Classification , DNA, Ribosomal , Fresh Water , Korea , Mortierella , Phylogeny , Pinus , Rhizosphere , Soil
Acta cir. bras ; 34(6): e201900606, 2019. graf
Article in English | LILACS | ID: biblio-1019270


Abstract Purpose To investigate the effects of pine needle extract (PNE) on the expression of proliferating cell nuclear antigen (PCNA) and Ki-67 during liver regeneration induced by 70% partial hepatectomy (PH) in rat. Methods Forty-eight male rats (SD, 7 weeks) had surgery (70% PH). They were randomly divided into two groups. PH + PNE group was only provided PNE diluted in water (10%) for drinking and PH group was provided water from 5 days before surgery to the time of sacrifice. PNE was made by pressing and filtering. Animals were sacrificed at 12h, 24h, 36h, 60h, 84h, 168h after PH, respectively. The expressions of PCNA and Ki-67 were determined as proliferation indices. Results Immunohistochemistry turned out to increase the expression of PCNA and Ki-67. PCNA expression of PH+PNE group increased up to twice of that of PH group. Western blot also seemed to increase the PCNA expression. These results indicated the promotion of cell proliferation in liver tissue and hepatic regeneration. Conclusions Pine needle extract stimulates the expression of some mitotic proteins during liver regeneration induced by 70% PH in rats. It suggests that administration of pine needle extract could accelerate the liver regeneration after partial hepatectomy.

Animals , Male , Rats , Plant Extracts/pharmacology , Proliferating Cell Nuclear Antigen/drug effects , Ki-67 Antigen/adverse effects , Pinus/chemistry , Hepatectomy/methods , Liver Regeneration/drug effects , Time Factors , Rats, Sprague-Dawley , Proliferating Cell Nuclear Antigen/metabolism , Ki-67 Antigen/metabolism , Cell Proliferation , Mitotic Index
Rev. biol. trop ; 66(4): 1580-1596, oct.-dic. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-1003348


Resumen Los estudios dendrocronológicos se utilizan para reconstruir algunas variables climáticas; en México, estos estudios se han centrado en los bosques templados del centro y del norte, donde los árboles presentan anillos anuales bien definidos. Pocos estudios se han llevado a cabo en la parte sur del país, donde el crecimiento anual del anillo no se identifica fácilmente y, por lo tanto, esto hace que la datación se dificulte. Se analizó el potencial dendrocronológico de Pinus oocarpa para la reconstrucción de variables climáticas en la porción noroeste de Lagunas de Montebello, Chiapas. Empleando un muestreo selectivo se recolectaron 65 núcleos de incremento de 34 árboles. Aunque las muestras presentaron una alta frecuencia de anillos falsos (8 a 60 %), se logró fechar 30 muestras de 22 árboles (46 %) mediante técnicas dendrocronológicas estándar y el desarrollo de cronologías de anillo total, madera temprana y madera tardía para un período de 91 años (1925-2015). Se encontró una influencia significativa de la precipitación media y de la temperatura media máxima y mínima del período 1961-2004 sobre el crecimiento anual de P. oocarpa. Los resultados muestran que la precipitación inviernoprimavera (enero-mayo) fue la más importante para el crecimiento del anillo anual de la especie. Sin embargo, la correlación más alta se observó entre la precipitación de primavera (marzo-mayo) y la cronología de la madera temprana (r = 0.719, P < 0.05). La cronología de la madera temprana también mostró potencial para reconstruir la temperatura mínima (marzo a mayo) (r = 0.732, P < 0.05), mientras que la cronología de madera tardía registra potencial para reconstruir la temperatura máxima (septiembreenero) (r = 0.714, P < 0.05). Estos resultados muestran que P. oocarpa puede emplearse para reconstruir variables climáticas en los trópicos mexicanos. Se recomienda explorar nuevas áreas con árboles más viejos a fin de aumentar la extensión de las cronologías y reconstruir los registros climáticos varios siglos en el pasado.(AU)

Abstract Dendrochronological studies are used to reconstruct some climatic variables; in México these studies have focused on central and Northern temperate forests where trees present well defined annual rings. Few studies have been carried out in the Southern part of the country where annual ring growth is not easily identified and thus makes cross-dating problematic. We analyzed the dendrochronological potential of Pinus oocarpa Schiede for reconstructing climatic variables in the Northwest portion of Lagunas de Montebello, Chiapas. We used a selective sampling approach and collected 65 increment cores from 34 trees. While our samples showed a high frequency of false rings (8 to 60 %), we were able to date 30 samples from 22 trees (46 %) using standard dendrochronological techniques and developed total chronologies for total ring width, earlywood, and latewood for a period of 91 years (1925-2015). We found a significant influence of mean precipitation and mean maximum and minimum temperature over the annual ring growth of P. oocarpa in the period 1961-2004. Our results show that winter-spring precipitation (January-May) was the most important for the species' annual ring growth. However, we found the highest correlation between spring (March-May) precipitation and the earlywood chronology (r = 0.719, P < 0.05). The earlywood chronology also showed potential for reconstructing minimum temperatures (March to May) (r = 0.732, P < 0.05), while the latewood chronology had the potential for reconstructing the maximum temperature (September to January) (r = 0.714, P < 0.05). These results showed that P. oocarpa can be used to reconstruct climatic variables in the Mexican tropics. We recommend that new areas with older trees should be explored in order to increase the depth of chronologies and reconstruct climate records several centuries into the past.(AU)

Wood , Climate Change , Pinus , Trees , Mexico
Bol. latinoam. Caribe plantas med. aromát ; 17(1): 53-60, ene. 2018. tab
Article in English | LILACS | ID: biblio-915115


This paper reports the chemical composition of essential oils obtained from Pinus dalatensis Ferré, Pinus kwangtungensis Chun ex. Tsiang and Pinus armandii subsp. xuannhaensis L.K. Phan. The oils were studied by gas chromatograpgy (GC) and gas chromatography coupled to mass spectrometry (GC-MS). The main constituents of P. dalatensis were the terpene hydrocarbons namely α-pinene (38.2%), ß- pinene (25.3%), ß-myrcene (11.0%) and ß-caryophyllene (10.5%), while α-cedrol (19.2%) was the only significant compound of P. armandi subsp. xuannhaensis. P. kwangtungensis showed ß-pinene (26.3%), α-pinene (18.0%), limonene (16.1%) and ß-myrcene (10.4%) as the dominant compounds. The volatile constituents of P. dalatensis and P. armandi subsp. xuannhaensis are being reported for the first time.

En este artículo se reportan los constituyentes químicos de los aceites esenciales de Pinus dalatensis Ferré, Pinus kwangtungensis Chun ex. Tsiang y Pinus armandii subsp. Xuannhaensis L.K. Phan que se analizaron mediante cromatografía de Gases (GC) y por Cromatografía de Gases acoplada a la Espectrometría de Masas (GC-EM). Los principales constituyentes de P. dalatensis fueron los hidrocarburos terpénicos, a saber, α-pineno (38.2%), ß-pineno (25.3%), ß-mirceno (11.0%) y ß-cariofileno (10.5%). Por otro lado, α- cedrol (19.2%) fue el único compuesto significativo de P. armandi subsp. Xuannhaensis mientras que el aceite de P. kwangtungensis estuvo dominado por ß-pineno (26.3%), α-pineno (18.0%), limoneno (16.1%) y ß-mirceno (10.4%). Los constituyentes volátiles de P. dalatensis y P. armandi subsp. xuannhaensis se informa por primera vez.

Oils, Volatile/chemistry , Pinus/chemistry , Terpenes/analysis , Vietnam , Gas Chromatography-Mass Spectrometry
Mycobiology ; : 138-146, 2018.
Article in English | WPRIM | ID: wpr-729786


Two-hundred and fifty-five strains of actinomycetes isolated from soil samples were screened for their antagonistic activities against four well-known wood decay fungi (WDF), including a brown rot fungus, Gloeophyllum trabeum and three white rot fungi Donkioporia expansa, Trametes versicolor, and Schizophyllum commune. A dual culture assay using culture media supplemented with heated or unheated culture filtrates of selected bacterial strains was used for the detection of their antimicrobial activity against four WDF. It was shown that Streptomyces atratus, S. tsukiyonensis, and Streptomyces sp. greatly inhibited the mycelial growth of the WDF tested compared with the control. To evaluate the biocontrol efficacy of S. atratus, S. tsukiyonensis, and Streptomyces sp., wood blocks of Pinus densiflora inoculated with three selected Streptomyces isolates were tested for weight loss, compression strength (perpendicular or parallel to the grain), bending strength, and chemical component changes. Of these three isolates used, Streptomyces sp. exhibited higher inhibitory activity against WDF, especially G. trabeum, as observed in mechanical and chemical change analyses. Scanning electron microscopy showed that cell walls of the wood block treated with Streptomyces strains were thicker and collapsed to a lesser extent than those of the non-treated control. Taken together, our findings indicate that Streptomyces sp. exhibits the potential to be used as a biocontrol agent for wood decay brown rot fungus that causes severe damage to coniferous woods.

Actinobacteria , Cell Wall , Tracheophyta , Culture Media , Fungi , Hot Temperature , Mass Screening , Microscopy, Electron, Scanning , Pinus , Schizophyllum , Soil , Streptomyces , Trametes , Weight Loss , Wood
Mycobiology ; : 388-395, 2018.
Article in English | WPRIM | ID: wpr-729737


Radionuclides were deposited at forest areas in eastern parts of Japan following the Fukushima Daiichi Nuclear Power Plant incident in March 2011. Ectomycorrhizal (EM) fungi have important effects on radiocaesium dynamics in forest ecosystems. We examined the effect of colonization by the EM fungus Astraeus hygrometricus on the uptake of cesium (Cs) and potassium (K) by Pinus densiflora seedlings. Pine seedlings exhibited enhanced growth after the EM formation due to the colonization by A. hygrometricus. Additionally, the shoot Cs concentration increased after the EM formation when Cs was not added to the medium. This suggests that A. hygrometricus might be able to solubilize Cs fixed to soil particles. Moreover, the shoot K concentration increased significantly after the EM formation when Cs was added. However, there were no significant differences in the root K concentration between EM and non-EM seedlings. These results suggest that different mechanisms control the transfer of Cs and K from the root to the shoot of pine seedlings.

Cesium , Colon , Ecosystem , Forests , Fungi , Japan , Nuclear Power Plants , Pinus , Potassium , Radioisotopes , Seedlings , Soil
IJB-Iranian Journal of Biotechnology. 2017; 15 (2): 95-101
in English | IMEMR | ID: emr-192447


Background: Nanoparticles have been applied to medicine, hygiene, pharmacy and dentistry, and will bring significant advances in the prevention, diagnosis, drug delivery and treatment of disease. Green synthesis of metal nanoparticles has a very important role in nanobiotechnology, allowing production of non-toxic and eco-friendly particles

Objectives: Green synthesis of silver nanoparticles [AgNPs] was studied using pine pollen as a novel, cost-effective, simple and non-hazardous bioresource. The antifungal activity of the synthesized AgNPs was investigated in vitro

Materials and Methods: Biosynthesis of AgNPs was conducted using pollen of pine [as a novel bioresource] acting as both reducing and capping agents. AgNPs were characterized using UV-visible spectroscopy, X-ray diffraction and transmission electron microscopy. In evaluation for antifungal properties, the synthesized AgNPs represented significant in vitro inhibitory effects on Neofusicoccum parvum cultures

Results: Pine pollen can mediate biosynthesis of colloidal AgNPs with an average size of 12 nm. AgNPs were formed at 22[degree]C and observed to be highly stable up to three months without precipitation or decreased antifungal property. AgNPs showed significant inhibitory effects against Neofusicoccum parvum

Conclusion: The first report for a low-cost, simple, well feasible and eco-friendly procedure for biosynthesis of AgNPs was presented. The synthesized AgNPs by pine pollen were nontoxic and eco-friendly, and can be employed for large-scale production. The nanoparticles showed strong effect on quantitative inhibition and disruption of antifungal growth

Antifungal Agents , Pollen , Nanoparticles , Pinus
Asia Pacific Allergy ; (4): 138-147, 2017.
Article in English | WPRIM | ID: wpr-750109


BACKGROUND: Pollen allergens are one of the main contributors to the development and/or aggravation of allergic rhinitis, allergic conjunctivitis, and asthma. OBJECTIVE: An examination of the airborne pollen in residential areas should be conducted to aid the diagnosis and treatment of allergic diseases. METHODS: Airborne pollen samples were collected from 2 sites in Incheon and 2 in Seoul from 2015 to 2016. RESULTS: The highest monthly concentration of airborne pollen was observed in May and September each year. Pollen from 32 families and 50 genera was identified over the 2 years; of these, Pinus spp. showed the highest pollen concentration (41.6%), followed by Quercus spp. (25.3%), and Humulus spp. (15.3%), the latter of which had the highest concentration among weed pollen. The total pollen concentration was significantly higher in Incheon than in Seoul (p = 0.001 in 2015, p < 0.001 in 2016) and higher in 2016 than in 2015. The concentrations of pollen from weed species (Cupressaceae, Humulus spp., Artemisia spp., Ambrosia spp., and Chenopodiaceae) and grass species (Gramineae) were significantly higher (p < 0.001) than those from tree species. Pollen was distributed from February to November. The first pollen identified in both regions in each year was Alnus spp. Overall, the total concentration of Quercus spp., Betula spp., Humulus spp., Artemisia spp., Ambrosia spp., and Chenopodiaceae pollen increased significantly over the 2 years. CONCLUSION: Region-specific differences exist in the pollen of major allergenic plants. Continuous monitoring of pollen is thus essential for management of pollen-related allergic disorders in each region.

Allergens , Alnus , Ambrosia , Artemisia , Asthma , Betula , Chenopodiaceae , Conjunctivitis, Allergic , Diagnosis , Humans , Humulus , Hypersensitivity , Pinus , Poaceae , Pollen , Quercus , Rhinitis, Allergic , Seoul , Trees
Braz. dent. sci ; 20(4): 93-99, 2017. ilus, tab
Article in English | LILACS, BBO | ID: biblio-877984


Objectives: The aim of the study was assess the knowledge and attitudes of students and dentists about the use and cementation of intra-radicular posts. Material and Methods: This cross-sectional study interviewed 150 students and 150 dentists in the city of Pelotas (Brazil). A questionnaire containing questions regarding restorative choice in teeth with large coronal destruction, level of confidence for the use of post and resin cement, function of intra-radicular posts and social-demographic characteristics was applied. Data were analysed using descriptive analysis and associations were tested through logistic regression using 95% confidence intervals. Results: The analysis showed that students presented an odd to choose prefabricated posts 127% greater than the dentists for anterior teeth, 105% greater to choose self-adhesive resin cement and 46% greater to choose conventional resin cement. Considering posterior teeth, students presented an odd to choose prefabricated posts 40% smaller than the dentists and an odd 51% greater to choose conventional resin cement. Conclusion: The formation level (students or dentists) was directly related to clinical choices of the interviewed. The use of prefabricated posts seems to be related to the location of the tooth in the arch, with pre-fabricated posts being more indicated for anterior teeth. Use of pre-fabricated posts in teeth with large coronal destruction is accepted in the literature. However, dentists are still not confident to use of pre-fabricated fiber posts especially in posterior teeth with large coronal destruction. (AU)

Objetivo: o objetivo do estudo foi avaliar o conhecimento e as atitudes dos alunos e dentistas sobre o uso e cimentação de pinos intra-radiculares. Material e Métodos: este estudo transversal entrevistou 150 estudantes e 150 dentistas na cidade de Pelotas (Brasil). Foi aplicado um questionário contendo perguntas sobre a escolha restauração em dentes com grande destruição coronária, nível de confiança para o uso de pinos e cimento resinoso, função dos pinos intra-radiculares e características sociodemográficas. Os dados foram analisados utilizando análise descritiva e as associações foram testadas através de regressão logística usando intervalos de confiança de 95%. Resultados: A análise mostrou que os alunos apresentaram uma chance para escolher pinos pré-fabricados 127% maior que os dentistas para dentes anteriores, 105% maior para escolher cimento resinoso autoadesivo e 46% maior para escolher o cimento resinoso convencional. Considerando os dentes posteriores, os estudantes apresentaram uma chance para escolher os pinos pré-fabricados 40% menor do que os dentistas e uma chance 51% maior para escolher o cimento resinoso convencional. Conclusão: O nível de formação (estudantes ou dentistas) está diretamente relacionado às escolhas clínicas dos entrevistados. O uso de pinos pré- fabricados parece estar relacionado à localização do dente no arco, com pinos pré-fabricados sendo mais indicados para os dentes anteriores. O uso de pinos pré-fabricados em dentes com grande destruição coronária é aceito na literatura. No entanto, os dentistas ainda não estão confiantes para o uso de pinos pré-fabricados de fibra, especialmente em dentes posteriores com grande destruição coronária. (AU)

Humans , Health Knowledge, Attitudes, Practice , Pinus , Resin Cements
Rev. cuba. plantas med ; 21(1): 96-107, jan.-mar. 2016. tab
Article in Spanish | LILACS, CUMED | ID: lil-781976


INTRODUCCIÓN: el follaje de Pinus caribaea Morelet var. caribaea (Barret y Golfari) presenta una variada composición química y es una planta forestal de abundancia en Cuba, que puede ser utilizada para la obtención de productos con actividad biológica, proporciona beneficios económicos, sociales y ambientales. OBJETIVO: evaluar el efecto citostático, citotóxico, antibacteriano y cicatrizante de cuatro extractos obtenidos del follaje verde de Pinus caribaea Morelet var. caribaea (Barret y Golfari) (pino macho). MÉTODOS: se determinó el efecto citostático mediante el ensayo de inhibición de la germinación de semillas de Solanum lycopersicum. La toxicidad se evaluó mediante el ensayo de letalidad frente a Artemia salina. La actividad antibacteriana se determinó in vitro mediante la técnica de Difusión en Agar por la aparición de halos de inhibición frente a tres cepas de microorganismos patógenos. El efecto cicatrizante se evaluó en un modelo in vivo en heridas abiertas en ratas. RESULTADOS: los resultados del presente trabajo indican que tres de los extractos evaluados mostraron toxicidad en ambos ensayos, donde Clorofilina de sodio constituyó la fracción más promisoria. Los extractos sin diluir de Pasta Clorofila Caroteno y Concentrado provitamínico mostraron actividad frente a Staphylococcus aureus, con valores que se encuentran en el límite mínimo que se considera con actividad antimicrobiana. Se comprobó el efecto cicatrizante de la Pasta Clorofila Caroteno a una dosis de 150 mg/mL. CONCLUSIONES: los resultados demostraron la actividad citostática y citotóxica de tres de los extractos evaluados. Los extractos de Pasta Clorofila Caroteno y Concentrado provitamínico mostraron actividad antibacteriana frente a Staphylococcus aureus y se demostró el efecto favorable de la Pasta Clorofila Caroteno en el proceso de curación de heridas abiertas, comparable a los efectos de medicamentos desarrollados con Rhizophora mangle L.

INTRODUCTION: Pinus caribaea Morelet var. caribaea (Barret y Golfari) foliage has a varied chemical composition and it is an abundant forest plant in Cuba, which can be used for the production of biological active products, it provides economic, social and environmental benefits. OBJECTIVE: to evaluate the cytostatic, cytotoxic, antibacterial and healing effect of four extracts obtained from green foliage of Pinus caribaea Morelet var. caribaea (Barret y Golfari) (male pine). METHODS: the cytostatic effect was determined using inhibition of germination of Solanum Lycopersicum seeds test. Toxicity was evaluated using Artemia salina lethality test. The antibacterial activity was determined in vitro using diffusion in agar technique by the appearance of inhibition halos against three strains of pathogenic microorganisms. The healing effect was evaluated performing an in vivo rat model in open wounds. RESULTS: the results of this study indicate that three of the tested extracts showed toxicity in both assays, where FI fraction was the most promising. Undiluted extracts of PCC and F-III showed activity against Staphylococcus aureus, with values considered in the lower limit for antimicrobial activity. The healing effect of the Carotene Chlorophyll Paste to a dose of 150 mg/mL was found. CONCLUSIONS: the results demonstrated cytostatic and cytotoxic activity of three extracts evaluated. Extracts of Carotene Chlorophyll Caste and Provitamin Concentrate showed antibacterial activity against Staphylococcus aureus and the favorable effect of the Carotene Chlorophyll Paste in the healing process of open wounds was demonstrated, comparable to the effect of drugs developed with Rhizophora mangle L.

Humans , Plants, Medicinal , Wound Healing , Pinus/toxicity , Anti-Bacterial Agents/therapeutic use , Cuba
PJMR-Pakistan Journal of Medical Research. 2016; 55 (1): 11-15
in English | IMEMR | ID: emr-179078


Background: Emergence of resistance to insecticides against mosquitoes and other disease vectors has prompted the need for alternate sources of mosquito control which are more target-specific, easily bio-degradable and effective against mosquitoes

Materials and Methods: The adulticidal efficacy of crude Neem oil extract, Pine oil extract and Til oil extract with five different concentrations i.e. 2%, 4%, 6%, and 8% was tested against five to six days old sugar fed female Aedes aegypti using standard WHO Bioassay test kit. The percentage mortality was observed after 24 and 48 hours under laboratory condition

Results: The LC[50] [Lethal Concentration] values of Neem oil extract against adults of Aedes aegypti were 1.17 ml and 0.09 ml after 24 and 48 hours of exposure respectively, while the LC[99] were 4.27 ml and 1.17 ml after 24 and 48 hours of exposure respectively. The LC[50] values of Pine oil extract after 24 and 48 hours were 0.25 and, 0.06 ml respectively, while LC[99] values at 24 and 48 hour were and 0.89 ml and 0.64 ml respectively. The LC[50] values of Til oil extract after 24 and 48 hours of exposure were 1.17 ml and 0.05 ml respectively while LC99 values were 4.18 ml and 1.28 ml after 24 and 48 hours of exposure respectively. The LC[50] and LC[90] values were indicative that the plant extracts were highly toxic after 48 hours of exposure. Among the tested plant oil extracts the maximum adulticidal efficacy was observed in the pine oil extract. No mortality was observed in controls

Conclusion: Crude extract of Pine oil was more effective against Aedes aegypti as compared to Neem and Til oil

Insecta , Plant Proteins , Calcium-Binding Proteins , Pinus , Plant Extracts , Plant Oils , Aedes/drug effects
Article in Chinese | WPRIM | ID: wpr-317772


<p><b>OBJECTIVE</b>This study aims to evaluate the effects of Pinus massoniana needle extract (PMNE) on inhibiting demineralization of root dentin.</p><p><b>METHODS</b>Root dentin blocks were randomly divided into distilled deionized water (DDW) group, fluoride sodium (NaF) group, and 4%, 8% and 12% PMNE groups according to the experimental solution used in the process of pH cycling in each group. All specimens in each group experienced pH cycling for 8 d. The dentin mineral density (DMD) of the normal dentin and demineralized dentin and their D-value (ΔDMD) were determined using micro computed tomography. The morphology of dentin surface after pH cycling was also observed using a scanning electron microscope.</p><p><b>RESULTS</b>The ΔDMD values in all PMNE groups and the NaF group were considerably lower than the ΔDMD in the DDW group (P<0.05). The ΔDMD values of the 8% and 12% PMNE groups had no difference (P>0.05), both of which were lower than the ΔDMD in the 4% PMNE group and higher than that in the NaF group (P<0.05). The dentin tubules were partly opened in the PMNE groups. The opening degrees of the dentin tubule in PMNE groups were significantly less and smaller than the opening degree in the DDW group and were larger than that in the NaF group.</p><p><b>CONCLUSIONS</b>PMNE can inhibit the deminera-lization of root dentin and can slow down the reduction in DMD. PMNE has the potential to prevent caries, and 8% PMNE can effectively inhibit dentin demineralization.</p>

Dental Caries , Dentin , Humans , Needles , Pinus , Sodium Fluoride , Tooth Demineralization , Tooth Root , X-Ray Microtomography
Mycobiology ; : 7-13, 2016.
Article in English | WPRIM | ID: wpr-729459


The genets of Suillus granulatus in a Pinus strobus stand (13 m × 60 m) were identified using random amplified polymorphic DNA molecular markers and the DNA of mushrooms that fruited for two years, and variations in genet size and distribution were analyzed. From a total of 116 mushrooms, 73 genets were identified and were grouped into three locations. The genets of mushrooms in close proximity differed from each other. The genet sizes varied at any of the three locations. The lengths of the identified genets in the pine stand ranged from 0.09 to 2.90 m. The average number of mushrooms per genet was 1.2 to 2.3, and the percentage of genets that were represented by a single mushroom was 44% to 94%. This variation in the genets of mushrooms in close proximity suggests that the ectomycorrhizal mycelial bodies of S. granulatus propagated sexually by fusing haploid spores derived from the mushrooms gills with below-ground mycelia. Therefore, it is necessary further to investigate the formation of new genets through spores in ectomycorrhizal fungal colonies.

Agaricales , Animals , DNA , Fruit , Gills , Haploidy , Pinus , Spores , Viverridae
Cienc. tecnol. salud ; 2(1): 47-52, ene.-jun. 2015. ilus, tab
Article in Spanish | LILACS | ID: biblio-834316


El objetivo principal fue la identificación de microorganismos del género Phytophthora que afectan a bosques naturales mixtos y viveros de Pinus sp. y Quercus sp., con importancia socioeconómica, en los departamentos de Guatemala y Sacatepéquez. Para el aislamiento de Phytophthora se procesaron muestras de suelo y tejido vegetal. En el departamento de Guatemala, del bosque natural se obtuvieron 10 muestras de Quercus sp. y 45 de Pinus sp., de los viveros se obtuvieron 11 muestras de Quercus sp. y 88 de Pinus sp. En el departamento de Sacatepéquez se obtuvieron del bosque natural 15 muestras de Quercus sp. y 48 de Pinus sp.; y en los viveros, 58 muestras de Pinus sp. y 25 de Quercus sp. Un total de 13 muestras procedentes de los viveros fueron positivas a la presencia de Phytophthora sp. en Pinus maximinoi, 10 procedentes del departamento de Guatemala, y tres de Sacatepéquez. Referente al tipo de crecimiento de la colonia en medio PDA, se obtuvieron cinco de tipo estolonífero, cinco tipo semipetaloide, una colonia de tipo estelado y dos colonias sin ningún tipo de patrón de crecimiento. Las pruebas de patogenicidad realizadas con la cepa VP16 mostraron alta incidencia y severidad para las especies de Pinus caribaea, P. oocarpa, P. pseudostrobus, P. maximinoi y en menor grado en Pinus tecunumanii.

The mean purpose of this research was to identify microorganism belonging to Phytophthora genera which are affecting mixed natural forests and nurseries of Pinus sp. and Quercus sp. These species have economic and social impact in provinces such as Guatemala and Sacatepéquez. Soil and vegetal tissue were used to isolate Phytophthora from natural forest of Guatemala, 45 Pinus sp. and 10 Quercus sp. were sampled and from nurseries 88 Pinus sp. and 11 Quercus sp. From Sacatepéquez province, from natural forest system were sampled 48 Pinus sp. and 15 Quercus sp. From nurseries were sampled 58 Pinus sp. and 25 Quercus sp. After processing the samples from soil and roots 13 were found positive to Phytophthora sp. in Pinus maximinoi, 10 from Guatemala and three from Sacatepéquez Provinces. The culture of Phytophthora sp. on PDA produced two colonies without define form and five stoloniferous, five semipetaloid, one stelade type colonies. VP16 isolate was inoculated in five species of pine for pathogenicity test, causing high percentages of incidence and severity on Pinus caribaea, P. oocarpa, P. pseudostrobus and P. maximinoi and low rates of incidence and severity on Pinus tecunumanii.

Humans , Animals , Male , Forests , Phytophthora , Pinus , Forests , Trees/microbiology
Article in English | WPRIM | ID: wpr-155590


Poria cocos is a well-known traditional Chinese traditional medicine (TCM) that grows around roots of pine trees in China, Korea, Japan, and North America. Poria cocos has been used in Asian countries to treat insomnia as either a single herb or part of an herbal formula. In a previous experiment, pachymic acid (PA), an active constituent of Poria cocos ethanol extract (PCE), increased pentobarbital-induced sleeping behaviors. The aim of this experiment was to evaluate whether or not PCE and PA modulate sleep architectures in rats as well as whether or not their effects are mediated through GABA(A)-ergic transmission. PCE and PA were orally administered to individual rats 7 days after surgical implantation of a transmitter, and sleep architectures were recorded by Telemetric Cortical encephalogram (EEG) upon oral administration of test drugs. PCE and PA increased total sleep time and non-rapid eye movement (NREM) sleep as well as reduced numbers of sleep/wake cycles recorded by EEG. Furthermore, PCE increased intracellular chloride levels, GAD65/67 protein levels, and alpha-, beta-, and gamma-subunits of GABA(A) receptors in primary cultured hypothalamic neuronal cells. These data suggest that PCE modulates sleep architectures via activation of GABA(A)-ergic systems. Further, as PA is an active component of PCE, they may have the same pharmacological effects.

Administration, Oral , Animals , Asian Continental Ancestry Group , China , Cocos , Electroencephalography , Ethanol , Eye Movements , Glutamate Decarboxylase , Humans , Japan , Korea , Medicine, Chinese Traditional , Neurons , North America , Pinus , Poria , Rats , Receptors, GABA-A , Sleep Initiation and Maintenance Disorders
Article in Chinese | WPRIM | ID: wpr-351281


Based on variation of Pinus massoniana families, heritablility and correlation analysis, the contents of shikimic acid and procyanidine (heritability 0.90, 0.70), dry weight of single branch (heritability 0.60) and and leaf length (heritability 0.46) were screened out as quality, yield and harvest cost traits of Folium Pini, respectively. For the different medicinal application of Folium Pini, varied methods were chosen to estimate weight and construct index equation. Weight adjustment based.on equal emphasis were used as economic weight determining method to select the best families, and the index (accuracy 0. 936 4 and heritability 0. 881 6) obtained was a little better than that obtained by equal emphasis, and much better than that by restricted index. The superior families selected with adjustment weight and equal emphasis were No. 46, 43 and 28. Partial regression were used as economic weight determining method to select the best families,and the index obtained had the highest accuracy (0.941 5) , index heritability (0. 889 9) and the genetic gain of shikimic acid content. The superior families selected with this method were No. 46, 27 and 47. No. 46 was the best families with maximal economic benefit. Our study indicated that suitable method for estimate weight and construct index equation can be applied for better accuracy of superior families selection of P. massoniana.

Breeding , Drugs, Chinese Herbal , Pinus , Chemistry , Classification , Genetics , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Classification , Genetics