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1.
Rev. méd. panacea ; 9(2): 82-89, mayo-ago. 2020. tab, graf
Article in Spanish | LILACS, LIPECS | ID: biblio-1121154

ABSTRACT

Objetivo: Describir los hallazgos hematológicos en donantes preseleccionados a plaquetoferesis sanguínea en el Hospital Nacional Edgardo Rebagliati Martins (HNERM) de Lima, Perú. Materiales y métodos: Estudio de tipo observacional, descriptivo y transversal, realizado entre Mayo-Julio 2019. Se incluyeron hemogramas de donantes que resultaron ̈Aptos ̈ a la entrevista médica, con resultado ̈Negativo ̈ a marcadores serológicos y que fueron diferidos en donar por presentar alteración hematológica. Los datos fueron recolectados del sistema informático del servicio de medicina transfusional del HNERM. Se utilizó estadística descriptiva y analítica (U de Mann Whitney y Chi-cuadrado de Pearson) considerándose un valor p<0,05 como significativo. Resultados: Se analizaron los hemogramas de 330 donantes preseleccionados diferidos, entre los cuales el recuento absoluto de eosinófilos (14,2%), el hematocrito (14,2%) y el recuento de plaquetas (33,9%) fueron las de mayor presencia. Asimismo, en las observaciones la fórmula leucocitaria invertida (20,6%) fue la más frecuente. Además, se observó diferencia estadística significativa (p<0,05) entre la hemoglobina, el hematocrito, la hemoglobina corpuscular media (HCM) y la fórmula leucocitaria invertida de acuerdo al sexo del donante. Conclusiones: La alteración hematológica observada más frecuentemente en esta población fue la plaquetopenia. Además, algunos parámetros de la serie roja y la distribución leucocitaria en el hemograma estuvieron asociados con el género del donante. (AU)


Objective: To describe the hematological findings in preselected donors to blood plateletpheresis at the Edgardo Rebagliati Martins National Hospital (HNERM) in Lima, Peru. Materials and methods: Observational, descriptive and cross-sectional study, carried out between May-July 2019. Blood counts were included from donors that were ̈Approved ̈ to the medical interview, with a ̈Negative ̈ result for serological markers and that were deferred in donating for present hematological alteration. The data was collected from the computer system of the HNERM transfusion medicine service. Descriptive and analytical statistics (Mann Whitney's U and Pearsonʼs Chi-square) were used, considering a p<0.05 as significant. Results: The blood counts of 330 delayed preselected donors were analyzed, among which the absolute eosinophil count (14.2%), the hematocrit (14.2%) and the platelet count (33.9%) were those with the highest presence. Likewise, in observations, the inverted white blood cell formula (20.6%) was the most frequent. In addition, a statistically significant difference (p<0.05) was observed between hemoglobin, hematocrit, mean corpuscular hemoglobin (MCH) and the inverted leukocyte formula according to the sex of the donor. Conclusions: The hematological alteration most frequently observed in this population was plateletpenia. In addition, some parameters of the red series and the leukocyte distribution on the blood count were associated with the gender of the donor. (AU)


Subject(s)
Humans , Male , Female , Blood Cell Count , Blood Donors , Plateletpheresis , Hematologic Diseases , Epidemiology, Descriptive , Cross-Sectional Studies , Observational Studies as Topic
2.
Article in Chinese | WPRIM | ID: wpr-774325

ABSTRACT

OBJECTIVE@#To estimate the size of HLA -Ⅰ class typed platelet apheresis donor bank.@*METHODS@#A total of 16062 blood samples from Chinese Han voluntary unrelated marrow donors in Jiangsu were included in this study. Luminex-SSO was used to detect the HLA -Ⅰ class(A,B locus) antigens. The probability of finding at least one HLA matched unrelated donor was calculated based on the HLA -I class phenotype frequency.@*RESULTS@#The population genetic data of HLA -Ⅰ class in Jiangsu were obtained, the optinal bans size in HLA typed apheresis plateler donor registry databane hrad been estimated by evaluating the population genetic data of HLA-1 class same donor.@*CONCLUSION@#The establishment of HLA-1 class typed apheresis platelet donor bank with a total size of 1500 persons is acceptable, which can satisty the patients with phenotype freguency>0.002 to find at least 1 phenotype same donor in 95% probavility.


Subject(s)
Bone Marrow , Bone Marrow Transplantation , HLA Antigens , Histocompatibility Testing , Humans , Plateletpheresis , Registries , Tissue Donors
3.
Article in Chinese | WPRIM | ID: wpr-771860

ABSTRACT

OBJECTIVE@#To understand the iron stores of the plateletpheresis donors, so as to provide some new experimental data for further exploration and more perfect health examination criteria of the plateletpheresis donors.@*METHODS@#A total of 297 plateletheresis donors conformed to standard in October 2018 were selected by the cross sectional study. The related factors affecting iron stores were analyzed; the effect of plateletpheresis times of donation on the levels of the hemoglobin(Hb) and serum ferritin(SF) as well as the iron deficency rate in the blood donors was also analyzed; the iron stores in the blood donors was evaluated.@*RESULTS@#The SF level in plateletpheresis donors negatively correlated with annual plateletphersis times of donation(r=-0.416, P<0.001); The SF level decreased with the increase of annual times of donation(P<0.05); The iron deficiency rate in plateletpheresis donors showed the increase trend with the increase of annual times of donation. The iron deficiency rate in male and femal with 18-23 times of donation was 12.5%(8/64) and 40%(6/15) respectively.@*CONCLUSION@#The blood center should reduce recruitment frequency and increase the testing of SF for regularly plateletpheresis donors.


Subject(s)
Blood Donors , Cross-Sectional Studies , Ferritins , Hemoglobins , Humans , Iron , Male , Plateletpheresis
4.
Journal of Experimental Hematology ; (6): 1993-1997, 2019.
Article in Chinese | WPRIM | ID: wpr-781506

ABSTRACT

OBJECTIVE@#To explore the effect of storage time on discharge and content of exosome from leukocyte-reduced apheresis platelets (LRA-Plt).@*METHODS@#Exosome (EXO) from LRA-Plt were acquired by ExoQuick, and its' morphology, immunological marker and particle size distribution were detected by transmission electron microscopy (TEM), Western blotting and dynamic light scattering (DLS), respectively. The changes in particle size distribution of EXO from LRA-Plt with different storage time were detected by DLS. The changes in content of protein and RNA of EXO from LRA-Plt with different storage time were detected by Nanodrop® ND-2000.@*RESULTS@#EXO from LRA-Plt was acquired successfully, which was characterized by cup-like shape, CD63/TSG101 enriched and Calnexin negative, and the particle size of which ranged from 30 to 200 nm. At early stored stage (stored for 1 day and 2 days), particle size of EXO from LRA-Plt was small and ranges from 30 to 40 nm. Meanwhile, the contents of protein and RNA were low. The particle size distribution, contents of protein and RNA of EXO from LRA-Plt were not significanty different ammg groups (P>0.05). At middle-late stored stage (stored for 3, 4 and 5 days), the particle size of EXO from LRA-Plt was larger than that of early stored stage, which ranges was from 130 to 200 nm. Meanwhile, the contents of protein and RNA were higher than those of early stored stage. Particle size distribution, contents of protein and RNA of EXO from LRA-Plt stored for middle-late stage were significant higher than those of early stored stage (P<0.05).@*CONCLUSION@#Morphology of EXO from LRA-Plt stored for middle-late stage was different from that stored for early stored stage. Moreover, the particle size distribution, contents of protein and RNA of EXO from LRA-Plt stored for middle-late stage were higher than those of early stored stage. A large amount of protein and RNA contained in EXO from LRA-Plt may participate in the multiple functions caused by platelet transfusion.


Subject(s)
Blood Platelets , Blood Preservation , Exosomes , Humans , Leukocytes , Patient Discharge , Platelet Transfusion , Plateletpheresis
5.
Journal of Experimental Hematology ; (6): 1607-1611, 2019.
Article in Chinese | WPRIM | ID: wpr-775677

ABSTRACT

OBJECTIVE@#To explore the effect of high volume platelet reduction therapy on the white blood cell (WBC) count and hemoglobin (Hb) level in patients with thrombocytosis.@*METHODS@#Thirty-two plateletphoreses were performed for patients with thromocytosis by using ELP or MNC program of blood component isolator of COBE spectra continuous flow concentrifugation and the ACD-A preservation solution for blood as blood anticoagulant. In each treatment of patients, 2.5-3.0 tines total blood volume (TBV) were circulated, then the platelet suspension of 1/5-1/4 time TBV was prepared and collected.@*RESULTS@#A single plateletpheresis took (212.53±41.54) minutes in which (8 812.63±2087.15) ml blood were treated, and (798.84±190.77) ml platelet suspension was collected. In the suspension, the platelet count was 4 486.50 (3 058.50-5 279.50)×10/L, containing 3 455.50 (2 288.68-4 226.71)×10. WBC count was 13.79 (10.21-20.72)×10/L, containing 11.90(7.81-14.40)×10. Hemoglobin concentration was (3.28±1.25) g/L,containing (2.62 ± 1.17) g. Before and after plateletpheresis, the patients' platelet count was 1 263.00 (1 052.50-1 807.50)×10/L and (778.83±247.25)×10/L(Z=4.94, P<0.01), WBC count was 9.96(6.44-14.01)×10/L and 8.59(5.37, 13.12)×10/L (Z=13.31, P<0.05), Hemoglobin concentration was (112.63 ± 24.56)g/L and (109.55 ± 24.46)g/L (t=1.68,P>0.05).@*CONCLUSION@#Using continuous flow centrifugation and blood component separating in plateletpheresis for the patients with thrombocytosis can obviously decrease the high ratio of platelets, and improve the effect of plateletpheresis. The high volume platelet reduction therapy can lead to decrease of WBC count to some alent, degree but WBC count still in the normal range, moreover not affect the hemoglobin level significantly.


Subject(s)
Hemoglobins , Humans , Leukocyte Count , Platelet Count , Plateletpheresis , Thrombocytosis
6.
Article in Chinese | WPRIM | ID: wpr-360040

ABSTRACT

<p><b>OBJECTIVE</b>To study the gene polymorphism distribution characteristics of human platelet HPA-1-5 and 15 blood group antigens and construct a certain scale of platelet HPA database in the north area of Henan Province so as to provide platelet apheresis for clinical departments.</p><p><b>METHODS</b>Using polymerase chain reaction with sequence-specific primers (PCR-SSP), the genotyping of HPA-1-5 and 15 system was carried out; the periperal blood of 500 healthy Han donors in north area of Henan Province was collected randomly, the gene and genotype frequencies were detected by direct counting method, and the population distribution frequncy of HPA genes was analyzed by Hardy-Weinberg balance test, and compared with other regions and ethnics by using χ(2) test.</p><p><b>RESULTS</b>There was statistically significant (P < 0.05) of increase HPA-3b and HPA-5a in North area of Henan Province, compared with Chinese Han population; the HPA-3b and 5a increase and HPA-2a decrease were statistically significant (P < 0.05), compared with Ethnic minority of China. There was partly increase of HPA-1a, 2a, 3a and 5a, compared with different regions and ethnic in abroad. HPA allele genes of 500 Han donors in the North area of Henan Province were as follows: 0.985 and 0.015 for 1a and 1b; 0.924 and 0.076 for 2a and 2b; 0.469 and 0.531 for 3a and 3b; 1.000 and 1.000 for 4a and 5a; 0.532 and 0.468 for 15a and 15b, respectively. HPA allele gene frequencies were 1aa0.970, 1ab0.030; 2aa0.848, 2ab0.152; 3aa0.222, 3ab0.494, 3bb0.284; 4aa1.000; 5aa1.000; 15aa0.282, 15ab0.500, 15bb0.218. Compared with other regions and ethnic, HPA gene frequencies partly had statistical significance.</p><p><b>CONCLUSION</b>Distribution of HPA allele frequencies in the North area of Henan province is in accordence with the Hardy-Weinberg law. There are race and regional differences in HPA allele gene frequencies, compared with other regions and countries. And the HPA systems HPA-3 and 15 display the genetic polymorphisms, which provides a theoretical basis for the relevant research of the same type platelet infusion and alloimmune thrombocytopenia.</p>


Subject(s)
Alleles , Antigens, CD , Genetics , Antigens, Human Platelet , Genetics , Blood Platelets , China , DNA Primers , Ethnic Groups , GPI-Linked Proteins , Genetics , Gene Frequency , Genotype , Humans , Neoplasm Proteins , Genetics , Plateletpheresis , Polymerase Chain Reaction , Polymorphism, Genetic
7.
Journal of Experimental Hematology ; (6): 1226-1231, 2016.
Article in Chinese | WPRIM | ID: wpr-246786

ABSTRACT

<p><b>OBJECTIVE</b>To explore the key technique for preparation of the frozen platelet and efficacy of its clinical application.</p><p><b>METHODS</b>The influences of the donators' peripheral platelet count, starting time of freeze, injection rate and evenness of the freeze-protective agent, storage mode, re-melting temperature and the capacity of water-bath etc. on the quality of the frozen platelets were analyzed retrospectively in 3 257 samples of frozen platelets before platelet pheresis. Then, the platelet counts were examined in 150 cases transfused with frozen platelets at the time-points of 1, 24, 48 and 72 hrs after transfusion, 90 cases suffered from the obstetrical bleeding were transfused with 200 parts of the re-melting frozen platelets, and then the peripheral blood platelet count, platelet increasing index(CCI), bleeding time and blood clot retraction rate etc. were observed for determining the clinical efficiency of the frozen platelets.</p><p><b>RESULTS</b>The floccule in the re-melting frozen platelets from the donators with (175-250)×10(9)/L platelets were decreased significantly(P<0.01). The quality of frozen platelets was influenced by the following factors, such as injection of DMSO at a too fast and heterogeneous rate, blood bags stored in a multilamminar space, and re-melting in a water-bath of small capacity etc. The routine storage for 0 and 3 days did not influence the quality of the frozen platelets. The recovery rate of one year-freezing platelets all was higher than 80%. The effects of the frozen platelets transfused into the patients with obstetrical bleeding displayed good haemostatic results, and the blood transfusion reaction did not occur. However, the frozen platelets immediately were exhausted and displayed their function, but the counting after 48 hrs could not display a good effect of raising platelet number.</p><p><b>CONCLUSIONS</b>The peripheral platelet count before platelet pheresis, the injection rate and evenness of the protective agent, the number of stratum for blood bags and the capacity of re-melting water-bath etc. all are the key factors influencing the quality of the frozen platelets. The frozen platelets prepared in this study shows a good efficacy of clinical application.</p>


Subject(s)
Blood Platelets , Blood Preservation , Blood Transfusion , Freezing , Hemostasis , Humans , Platelet Count , Platelet Transfusion , Plateletpheresis , Transfusion Reaction
8.
Article in Chinese | WPRIM | ID: wpr-357258

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the changes of physiological activities and functions in vitro of apheresis platelets during storage.</p><p><b>METHOD</b>17 units of apheresis platelets were randomly chosen and stored at 20 °C to 24 °C with agitation. Platelet counting (Plt), mean platelet volume (MPV), blood gases, pH value, glucose (Glu) concentration, lactate (LA) concentration, LDH concentration, thromboelastogram (TEG), hypotonic shock response (HSR), CD62p expression rate and anew expression rate were measured on days 0, 1, 3, 5 after platelet storage. Changes of physiological activities and functions in vitro were systematically evaluated by above-mentioned indexes.</p><p><b>RESULTS</b>During storage, Plt, MPV and HSR were not significantly changed; but pH value, blood gases, Glu, LA, LDH, HSR, expression rate of CD62p and anew expression rate were significant differenty. Among thromboelastogram indexes, R value increased obviously with prolongation of storage time; K value and αAngle were not significantly changed; MA was not significantly changed on day 1 and 3, but was slightly increase on day 5.</p><p><b>CONCLUSION</b>The physiological activities and functions in vitro of apheresis platelets are kept well during storage. For clinical transfusion of apheresis platelet during storage, clinical effect of transfusione is not influenced.</p>


Subject(s)
Blood Platelets , Blood Preservation , Humans , In Vitro Techniques , Platelet Count , Plateletpheresis , Thrombelastography
9.
Article in English | WPRIM | ID: wpr-215691

ABSTRACT

BACKGROUND: While plateletpheresis donation results in less red blood cell loss and therefore less depletion of storage iron, repeated plateletpheresis can also lead to iron depletion. To determine the safety of regular plateletpheresis donations, this study estimated donor's iron status according to age, gender, number of donations, and donation interval. METHODS: The study population included 5,109 plateletpheresis donors (4,824 males, 285 females), who passed the hemoglobin (Hb) criteria for plateletpheresis donation of 12.0 g/dL or more in an inclusion period (September 2013~November 2013). During donor screening, serum ferritin levels were measured for assessment of iron status of plateletpheresis donors. RESULTS: Mean age of donors was 30.4 years (range: 17~59). Donors with a history of donation of more than 3 years accounted for 89.3% and 74.0% in males and females, respectively. Mean donation interval and annual donation number in male (female) donors was 11.9 (7.2) weeks and 4.2 (8.7) times, respectively. Approximately 37.8% of male donors and 64.2% of female donors had a serum ferritin level of less than 15 ng/mL. Serum ferritin levels showed correlation with donation interval, as the percentage of donors with a low ferritin level decreased with increase in donation interval (rho: 0.191~0.438, P<0.001). Serum ferritin levels also showed correlation with annual plateletpheresis number (rho: -0.261~-0.411, P<0.001). CONCLUSION: Depleted iron store was observed in nearly 40% of donors who had acceptable Hb levels for plateletpheresis donation. Hb pre-donation screening is not sufficient to reduce the risk of iron deficiency in regular plateletpheresis donors.


Subject(s)
Anemia, Iron-Deficiency , Blood Donors , Donor Selection , Erythrocytes , Female , Ferritins , Humans , Iron , Male , Mass Screening , Plateletpheresis , Tissue Donors
10.
Article in Korean | WPRIM | ID: wpr-215690

ABSTRACT

BACKGROUND: Transfusion of HLA-matched platelets is required when development of platelet refractoriness occurs after repeated platelet transfusion. This study was conducted to establish a HLA-matched platelet donor registry to supply matched platelets to patients who develop platelet refractoriness. METHODS: HLA-matched platelet donors were recruited among plateletpheresis donors. HLA-A and HLA-B antigen types of recruited donors were tested using a polymerase chain reaction-sequence specific oligonucleotide probe method. RESULTS: A total of 1,029 plateletpheresis donors were recruited. HLA-A and HLA-B antigen frequencies of recruited donors were similar to those of previously reported HLA antigen frequencies of Koreans. During the study period, a patient with platelet refractoriness recovered after receiving six units of HLA-matched platelets. CONCLUSION: During this study 1,029 donors were registered as HLA-matched platelet donors and a patient with platelet refractoriness received HLA-matched platelets using this registry. Supply of HLA-matched platelets will be facilitated by continuous expansion of the number of registered HLA-matched platelet donors, development of a program for management and searching for HLA-matched donors, and establishment of a request-supply system between hospitals and the Korean Red Cross through further studies.


Subject(s)
Blood Platelets , HLA-A Antigens , HLA-B Antigens , Humans , Platelet Transfusion , Plateletpheresis , Red Cross , Tissue Donors
11.
Rev. bras. hematol. hemoter ; 36(3): 191-195, May-Jun/2014. tab
Article in English | LILACS | ID: lil-713679

ABSTRACT

OBJECTIVE: To recognize the profile of platelet donors and the profile of the plateletpheresis session as well as to investigate the main adverse events of platelet donation using plateletpheresis and associated risk factors. METHODS: This retrospective, cross-sectional and analytical study was performed with a quantitative approach by analyzing 316 donation files from February 2010 to December 2011. The IBM SPSS Statistics program was used for data processing and analysis. The chi-square test was used to verify whether there was an association between factors related to the procedure and the donor, and the adverse events that occurred. RESULTS: The mean age of platelet donors was 40 years old (standard deviation = 8.9), with the prevalent age group being between 40 and 49 years old; the prevalent blood type was O positive (53.8%), the mean duration of the procedure was 73 min and the mean amount of anticoagulant used was 360 mL. The association between procedure duration and the volume of anticoagulant was inverse and statistically significant; the longer the procedure and the greater the volume of anticoagulant used, the less adverse reactions occurred. CONCLUSION: The low incidence of adverse events indicates that the procedure is well tolerated by donors. Obtaining data regarding the incidence of adverse events is a way of promoting a dynamic review of medical and nursing teams to improve the safety and comfort of the donor. .


Subject(s)
Humans , Male , Female , Paresthesia , Risk Factors , Plateletpheresis , Syncope, Vasovagal
12.
Article in Chinese | WPRIM | ID: wpr-349678

ABSTRACT

This study was aimed to evaluate the efficiency and effectiveness of platelet-rich plasma(PRP) prepared by acute plateletpheresis in patients undergoing open heart surgery, and to analyze the quality of prepared platelet-rich plasma. Whole blood from 20 patients with ASAII-III was collected and PRP was harvested by machine after induction of anesthesia. Platelet count (Plt), mean platelet volume (MPV), platelet distribution width (PDW), plasma pH, plasma lactic acid (LA) concentration, and lactic dehydrogenase (LDH) concentration, germiculture result, CD62p and PAC-1 positive rate of inactivated and activated platelets by ADP in the whole blood before plateletpheresis (T1) , in the PRP after plateletpheresis (T2) and PRP before back-transfusion (T3) were determinated. The results showed that as compared with whole blood the platelet count in the PRP at T2 was (783 ± 184) ×10(9)/L, MPV, PDW and pH significantly decreased (P < 0.01) , while the plasma LDH, LA concentration, CD62p and PAC-1 positive rate of inactivated platelets were not significantly different from the whole blood at T1. In the PRP at T3, the platelet count, MPV, PDW and pH significantly decreased (P < 0.01) , while plasma LDH concentration, CD62p and PAC-1 positive rate of inactivated platelet significantly increased (P < 0.05 or P < 0.01) compared with the whole blood at T1. There were no significant difference among the CD62p and PAC-1 positive rate of activated platelets in the whole blood and PRP. It is concluded that PRP can be efficiently obtained from the patients undergoing open heart surgery by acute plateletpheresis, and the platelets in PRP are not activated during the preparing process. Some platelets in PRP are activated during the preserving process, but the whole activating function of platelets keeps normal.


Subject(s)
Adult , Aged , Cardiac Surgical Procedures , Methods , Humans , Middle Aged , Platelet-Rich Plasma , Plateletpheresis , Methods
13.
Rev. argent. transfus ; 39(4): 235-246, 2013.
Article in Spanish | LILACS | ID: lil-727287

ABSTRACT

En la actualidad disponemos de tres tipos de concentrados de plaquetas (CP): CP procesados por el método de plasma rico en plaquetas (CP-PRP) o por el método de capa leucocitaria "buffycoat" (CP-BC) a partir de unidades de sangre entera donadas al azar (CPR) y CP obtenido por aféresis (CP-aféresis). La calidad y caracerísticas de las plaquetas durante el almacenamiento se afectan por una serie de factores, tales como el anticoagulante, la centrifugación y procesamiento después de la colecta, y el agrupamiento antes o después del almacenamiento. Por último, el uso de cada uno de estos componentes ya sean originales, o leucorreducidos, o suspendidos en solución de almacenamiento, o procesados con una técnica de inactivacion de patógenos agrega nuevos factores de complejidad para compararlos. Aunque está claro que el CP-BC retiene mucho más funciones in vitro que el CP-PRP, lo que indica que no se debería utilizar más este último, es mucho más difícil encontrar diferencias con los CP-aféresis. Otro factor que puede afectar la decisión política es la aparición de reacciones adversas en los receptores. Si se consideran solamente los datos comparables, por ejemplo CPR leucorreducida en comparación con CP-aféresis leucorreducida, hay pruebas de que este último está más asociado con reacciones adversas en los receptores. Muy pocos estudios se han publicado comparando la eficacia clínica de los CPR frente a CP-aféresis, considerando como resultado final principalmente el incremento del recuento corregido de plaquetas (IRC). De manera similar a los estudios in vitro, aunque el CP-PRP muestra IRC más bajos, no existe una diferencia clara entre CP-BC y CP-aféresis. Otros aspectos que pueden afectar la decisión es el hecho de que el uso de CP-aféresis en lugar de CPR reduce la exposición total de los pacientes a los donantes, lo cual se considera crítico en algunos países para reducir el riesgo de transmisión de infecciones transmisibles por la sangre...


Subject(s)
Blood Buffy Coat , Platelet Transfusion , Plateletpheresis/methods , Plateletpheresis/standards , Blood Component Removal , Blood Platelets , Communicable Diseases , Blood Preservation/methods , Platelet-Rich Plasma , Risk
14.
Article in Chinese | WPRIM | ID: wpr-313956

ABSTRACT

This study was aimed to evaluate the impact of regular donating platelets on serum ferritin (SF) of donors. A total of 93 male blood donors including 24 initial plateletpheresis donors and 69 regular plateletpheresis donors were selected randomly. Their SF level was measured by ELISA. The results showed that the SF level of initial plateletpheresis donors and regular plateletpheresis donors were 91.08 ± 23.38 µg/L and 57.16 ± 35.48 µg/L respectively, and all were in normal levels, but there was significant difference between the 2 groups (p < 0.05). The SF level decreased when the donation frequency increased, there were no significant differences between the groups with different donation frequency. Correlation with lifetime donations of platelets was not found. It is concluded that regular plateletpheresis donors may have lower SF level.


Subject(s)
Adult , Blood Donors , Ferritins , Blood , Humans , Iron , Blood , Male , Middle Aged , Platelet Count , Plateletpheresis , Serum , Chemistry , Young Adult
15.
Journal of Experimental Hematology ; (6): 1046-1050, 2010.
Article in Chinese | WPRIM | ID: wpr-237598

ABSTRACT

This study was purposed to determine the mode and size of human platelet antigens (HPA) typed platelet apheresis donor bank. The published data of HPA distribution collected from Chinese Han population of 16 provinces were analyzed. The combined data were tested with the Hardy-Weinberg equilibrium. The results showed that the bb homozygote was not detected in HPA-1, -4, -6, -10, and b gene was not found in HPA-7-9, 11-14, -16. There were 648 combined HPA 1-16 genotypes in Chinese Han population, and the cumulative frequency of 42 combinations higher than 0.001 were 0.9763. The highest frequency (0.2012) in combination was HPA-(7-8-9-11-12-13-14-16) aa - (1-4-5-6-10) aa-2aa-3ab-15ab. The probability of HPA dual antigen mismatch in HPA-15, -3 and -2 was higher than the 0.1, and the probability in the HPA-1, -5, and -6 was between 0.01 - 0.1. The probability of full-match in HPA1-16 antigens was 0.3195 in Chinese Han population after the random blood transfusion. According to the curve drawn by donor number (N) versus frequency (F), the regression equation LogN = -0.4394 x Ln (F) +0.4324 was derived at P = 95%. If the derived frequency (product of HPA frequency and ABO frequency) is 0.005, then the N should be 576.07 at least in Chinese Han population. It is concluded that the mode of regional, multi-center database of HPA-typed platelet apheresis donor bank may be acceptable in Chinese Han population, and the suitable number of HPA-typed platelet donor in one bank may be 600. Therefore, the bank can be used to treat the platelet transfusion refractoriness (PTR) caused by HPA-15, 3 and 2 mismatch mainly, and can be expanded effectively in similar genetic background to deal with the low-frequency HPA antigens mismatch. The number of HPA-typed platelet apheresis donor influences not only on the frequency of HPA, but also on the frequency of ABO group.


Subject(s)
Antigens, Human Platelet , Genetics , Allergy and Immunology , Asian Continental Ancestry Group , Genetics , Blood Donors , Gene Frequency , Genotype , Histocompatibility Testing , Humans , Platelet Transfusion , Plateletpheresis
16.
Journal of Experimental Hematology ; (6): 1059-1062, 2010.
Article in Chinese | WPRIM | ID: wpr-237595

ABSTRACT

This study was purposed to confirm the practical efficacy of reducing indicating germs suspended in plasma by riboflavin and photosensitized inactivation and to evaluate its influence on activation of apheresis platelet concentrates. The synergistic effects of riboflavin combined with ultraviolet irradiation on inactivation of germs were investigated by using Escherichia Coli (E. coli) and Staphylococcus Aureus (S. aureus) as Gram⁻ and Gram(+) indicating germs, respectively. The activation status of apheresis-platelet concentrates treated with riboflavin combined with ultraviolet irradiation was detected by flow cytometry. The results showed that when 50 μmol/L of riboflavin was combined with 6.2 J/ml of ultraviolet irradiation, the T/E ratios reached 1.42 for E. coli and 1.68 for S. Aureus, and reduction of E. Coli and S. Aureus were 3.87 Logs and 3.82 Logs respectively; the CD62p expression level on germ-inactivated platelets stored at 22 degrees C for 0 and 5 days were 4.92% and 36.18% respectively, which slightly increased as compared with controls (3.94% and 32.03)% (p < 0.05). It is concluded that combination of riboflavin with ultraviolet irradiation displays well synergistic effects which can reduce E. Coli and S. Aureus counts, but no significantly influence on platelets. The partial activation of liquid platelets mainly presents metabolism damage during storage, which is found at an acceptable level.


Subject(s)
Blood Platelets , Metabolism , Drug Carriers , Gram-Negative Bacteria , Radiation Effects , Gram-Positive Bacteria , Radiation Effects , Humans , P-Selectin , Blood , Photosensitizing Agents , Pharmacology , Platelet Count , Plateletpheresis , Methods , Riboflavin , Pharmacology , Ultraviolet Rays
17.
Article in Chinese | WPRIM | ID: wpr-243327

ABSTRACT

This study was purposed to investigate the effects of 25 Gy gamma-ray irradiation on the CD62p expression, platelet count and the mean platelet volume (MPV) of manually enriched platelet suspension in different time of shelf life at 22 degrees C. Each of 16 bags with plasma-rich platelet was divided into two bags, one of which was exposed to 25 Gy gamma-ray of 137Cs and the other ones was not exposed. 16 bags then were preserved for 72 hours according to AABB standards. The irradiated platelets were regarded as the observation group, and the other ones were regarded as the control group, the expression of p-selectin (CD62p) in the above 2 groups was detected by flow cytometry before irradiation and at 24, 72 hours after irradiation respectively; at the same time, the platelet count and MPV were assayed by using blood cell counter. The results showed that the expression level of CD62p on platelet in irradiated and control groups increased along with the prolonging of preservation time, the expression rate of CD62p on the platelets preserved for 24 hours was higher than that on fresh platelets with significant difference (p<0.05); the expression rate of CD62p on the platelets preserved for 72 hours obviously was enhanced as compared with platelets preserved for 24 hours (p<0.01). There were no significant differences in CD62p expression rate, platelet count and MPV between irradiated and control groups preserved for 24 and 72 hours (p>0.05), however the MPV of irradiated and control groups preserved for 72 hours was higher than that of fresh platelets (p<0.05). It is concluded that the gamma-ray irradiation does not affect the quantity and quality of platelets, but the preservation time for manually enriched platelet suspension should be shortened as far as possible.


Subject(s)
Blood Platelets , Metabolism , Radiation Effects , Flow Cytometry , Gamma Rays , Humans , P-Selectin , Metabolism , Radiation Effects , Platelet Count , Plateletpheresis , Preservation, Biological , Methods
18.
Journal of Experimental Hematology ; (6): 1067-1070, 2009.
Article in Chinese | WPRIM | ID: wpr-343347

ABSTRACT

This study was aimed to investigate the effect of leukocyte depletion by filtration on the quality of apheresis platelets. 20 units of donor apheresis platelets were randomly selected and were preserved with agitation at 20 - 24 degrees C for 24 - 96 hours, then were filtered on polyester flatbed filters. The platelet concentration, mean platelet volume (MPV), volume of apheresis platelets, leukocyte count, pH value, lactate dehydrogenase (LDH) concentration, K(+) concentration and CD62p expression level on surface of platelet membrane, were detected before and after filtration, as well as the rate of leukocyte depletion and platelet loss were calculated. The results showed that the leukocyte count after filtration was remarkably lower than that before filtration (p < 0.001), and the rate of leukocyte depletion was 99.97%. Platelet loss was approximately 8%, and obviously lower than that of the national standard (p < 0.001). MPV, pH value, K(+) and LDH concentration were not significantly different before and after filtration. Compared with platelets before filtration, CD62p expression level after filtration slightly decreased (p > 0.05). CD62p expression on surface of platelet membrane in perfusion fluid obtained from filter plate was obviously higher than that before filtration (p < 0.05). MA of platelet after filtration slightly decreased (p > 0.05). It is concluded that leukocyte and partial activated platelets can be removed efficiently by using polyester flatbed filters, and platelet loss is very low. Filtration does not adversely affect coagulation activity of the platelets in vitro. Apheresis platelets after filtration can fulfil quality requirements to prevent infection of cytomegalovirus and HLA alloimmunization.


Subject(s)
Filtration , Humans , Leukapheresis , Platelet Count , Plateletpheresis , Methods
19.
Article in Korean | WPRIM | ID: wpr-179779

ABSTRACT

BACKGROUND: Several kinds of adverse reactions can occur during blood donation such as vasovagal reaction (VVR), hematoma, citrate toxicity, etc. These adverse reactions are not common, but they are important because they cause a decrease in re-donation. The cost for maintaining a repeat donation is very low compared to that for securing first-time donors. Whole blood donation differs from apheresis in some aspects, and this could have an influence on blood donor reactions. We compared whole blood donation with apheresis for blood donor reactions. METHODS: From January to December in 2007 at Busan Red Cross Blood Center, 109,004 donations were investigated for blood donor reactions. 76,098 (69.8%) donations were from male donors and 32,906 (30.2%) were from females. 77,813 (71.3%) donations were for whole blood, 25,224 (23.2%) were for plasmapheresis and 5,967 (5.5%) were for plateletpheresis. RESULTS: The frequencies of VVR were 0.10% (75/77,813) for the whole blood donations, 0.15% (37/25,224) for plasmapheresis and 0.03% (2/5,967) for plateletpheresis (P<0.05). The frequency of hematoma was 0.05% (37/77,813) for whole blood donation, 0.25% (62/25,224) for plasmapheresis and 0.27% (16/5,967) for plateletpheresis (P<0.05). Citrate toxicity was extremely rare. VVR was most common in plasmapheresis, and it was rare in plateletpheresis. CONCLUSION: The kinds of donated blood components had an influence on blood donor reactions. Understanding these characteristics helps to prevent adverse reaction. Having people re-donate is essential for keeping a large sized donor pool. So, appropriate management to prevent donor reactions is very important.


Subject(s)
Blood Component Removal , Blood Donors , Citric Acid , Female , Hematoma , Humans , Male , Plasmapheresis , Plateletpheresis , Red Cross , Tissue Donors
20.
Article in Korean | WPRIM | ID: wpr-171235

ABSTRACT

Glanzmann's thrombasthenia is an autosomal recessive bleeding disorder caused by qualitative or quantitative abnormalities of the platelet glycoprotein IIb/IIIa (GP IIb/IIIa), which can lead to excessive bleeding. Glanzmann thrombasthenia is associated with clinical variability, with some patients only having minimal bruising and others having frequent, severe and potentially fatal hemorrhages. Platelet transfusions, which used to be the standard treatment, may lead to the development of antibodies to HLA and/or GPIIb/IIIa, thereby rendering future transfusions ineffective. Glanzmann's thrombasthenia can be a severe hemorrhagic disease; however, the prognosis is excellent with careful supportive care. In this case, administering allogenic plateletpheresis to patients with Glanzmann's thrombasthenia who were refractory to platelet transfusions was found to be successful during bone surgeries.


Subject(s)
Anesthesia, General , Antibodies , Blood Platelets , Glycoproteins , Hemorrhage , Humans , Orthopedics , Platelet Transfusion , Plateletpheresis , Prognosis , Thrombasthenia
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