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Rev. Soc. Odontol. La Plata ; 31(61): 7-12, 2021. ilus, graf
Article in Spanish | LILACS | ID: biblio-1353793


Este trabajo presenta los resultados de un estudio de envejecimiento natural y acelerado en saliva artificial, de un material para uso odontológico de la marca Deflex® a base de poliamida de alto impacto, según lo establece la norma UNE EN IS0 10993-13 (2009) (AU)

is work presents the results of the study of natural and accelerated aging in artificial saliva, of a material for dental use of the brand Deflex® based on high impact polyamide, as established by the standard UNE EN IS0 10993-13 (2009) (AU)

Saliva, Artificial , Aging , Nylons , Polymers/chemistry , Spectrum Analysis/methods , Materials Testing , Spectroscopy, Fourier Transform Infrared
Bol. latinoam. Caribe plantas med. aromát ; 19(1): 65-76, ene. 2020. tab, ilus
Article in English | LILACS | ID: biblio-1102867


Due to the biological activities of Syzygium aromaticum essential oil, its incorporation in methacrylate polymeric (Eudragit E100) nanoparticles (NP), physical characterization, and antimicrobial essays were evaluated. The clove bears great potential for applications in dentistry. The oil was obtained by hydrodistillation and oil loaded NP using the nanoprecipitation method. Particle size and polydispersity index were determined by photon correlation spectroscopy, and physical morphology by electron microscopy. Loading capacity and in vitro eugenol release were evaluated by gas mass chromatography, and the antimicrobial activity of oil loaded-NP was calculated against Streptococcus mutans. Different chemical ingredients were characterized, and eugenol was the principal compound with 51.55%. Polymer content was directly related to NP homogenous size, which was around 150 nm with spherical morphology. A 73.2% loading capacity of eugenol was obtained. Oil loaded NP presented a fickian-type release mechanism of eugenol. Antimicrobial activity to 300 µg/mL was obtained after 24 h.

Debido a las actividades biológicas del aceite esencial de Syzygium aromaticum, se evaluó su incorporación en nanopartículas (NP) de metacrilato polimérico (Eudragit E100), su caracterización y ensayos antimicrobianos. El clavo tiene un gran potencial para aplicaciones en odontología. El aceite se obtuvo por hidrodestilación y las NP cargado de aceite utilizando el método de nanoprecipitación. El tamaño de partícula y el índice de polidispersidad se determinaron mediante espectroscopia de correlación fotónica y su morfología por microscopía electrónica. La capacidad de carga y la liberación de eugenol in vitro se evaluaron mediante cromatografía de gases en masa, y la actividad antimicrobiana se evaluó contra Streptococcus mutans. Se caracterizaron diferentes ingredientes químicos, siendo el eugenol el principal compuesto con 51.55%. El contenido de polímero se relacionó directamente con el tamaño homogéneo de NP, que fue de alrededor de 150 nm con morfología esférica. Se obtuvo un 73,2% de capacidad de carga de eugenol. El aceite cargado en NP presentó un mecanismo de liberación de eugenol de tipo fickiano. La actividad antimicrobiana a 300 µg/mL se obtuvo después de 24 h.

Polymers/chemistry , Oils, Volatile/administration & dosage , Syzygium/chemistry , Nanoparticles/chemistry , Anti-Bacterial Agents/administration & dosage , Streptococcus mutans/drug effects , Eugenol/pharmacology , Oils, Volatile/pharmacology , Administration, Oral , Chromatography, Thin Layer , Drug Delivery Systems , Gas Chromatography-Mass Spectrometry , Anti-Bacterial Agents/pharmacology
Braz. arch. biol. technol ; 63: e20200022, 2020. tab, graf
Article in English | LILACS | ID: biblio-1132196


Abstract Despite its potential in the production of polymers from renewable sources, D-limonene faces difficulties in its polymerization, resulting in low monomer conversion and molar mass. In order to investigate the non-ideality inherent kinetics, this work explores different modeling strategies for D-limonene radical polymerization, using benzoyl peroxide as initiator. The starting model considered the classical approach for conventional radical polymerization. This model was then corrected by including reaction orders different from the unit. After an analysis and choice of the best model, computer simulations were compared with experimental results from literature, validating the chosen approach. It was found that the process is drastically influenced by chain transfer reactions, presenting a non-ideal behavior. Finally, an analysis of distinct reaction conditions provided information on monomer conversion, molar mass and polymer dispersity, which could guide future research in the synthesis optimization. Higher molar mass poly(limonene) were obtained by simultaneously reducing the monomer and initiator concentrations.

Polymers/chemistry , Kinetics , Limonene , Styrene , Polymerization , Chemistry Techniques, Synthetic , Models, Theoretical
Braz. oral res. (Online) ; 34: e095, 2020. tab, graf
Article in English | LILACS, BBO | ID: biblio-1132703


Abstract The purpose of this study was to synthesize dicalcium phosphate dihydrate (DCPD) particles functionalized with triethylene glycol dimethacrylate (TEGDMA) through different routes by varying the receptor solution: ammonium phosphate (AP groups) or calcium nitrate (CN groups) and the moment in which TEGDMA was incorporated: ab initio (ab) or at the end of dripping the solution (ap). Two syntheses were performed without adding TEGDMA (nf). The particles were characterized by X-ray diffractometry, true density (using a helium pycnometer), surface area, and scanning electron microscopy. A 20 vol% of DCPD particles from the D, E, and F groups was added to the resin matrix to determine the degree of conversion (DC), biaxial flexural strength (BFS), the flexural modulus (FM), and surface roughness after an abrasive challenge (RA). A group with silanized barium glass particles was tested as a control. The data were submitted to ANOVA/Tukey's test (DC, BFS, and RA), and the Kruskal-Wallis test (FM) (alpha = 0.05). BFS values varied between 83 and 142 MPa, and the CN_ab group presented a similar value (123 MPa) to the control group. FM values varied between 3.6 and 8.7 GPa (CN_ab and CN_nf groups, respectively), with a significant difference found only between these groups. RA did not result in significant differences. The use of calcium nitrate solution as a receptor, together with ab initio functionalization formed particles with larger surface areas. Higher BFS values were observed for the material containing DCPD particles with a higher surface area. In general, the DC, FM, and RA values were not affected by the variables studied.

Polymers/chemistry , Composite Resins , Flexural Strength
Braz. oral res. (Online) ; 33: e026, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001604


Abstract: The aim of this study is to evaluate the machinability of four CAD/CAM materials (n = 13) assessed by brittleness index, Vickers hardness, and fracture toughness and interaction among such mechanical properties. The materials selected in this in vitro study are Feldspathic ceramic [FC], Lithium-disilicate glass ceramic [LD], leucite-reinforced glass ceramic [LR], and nanofilled resin material [RN]. Slices were made from the blocks following original dimensions 14 × 12 × 3 mm (L × W × H), using a precision slow-speed saw device and then surfaces were regularized through a polishing device. Brittleness index and fracture toughness were calculated by the use of specific equations for each one of the properties. The Vickers hardness was calculated automated software in the microhardness device. One-way Anova and Pearson's correlation were applied to data evaluation. LD obtained the highest values for brittleness index and was not significantly different from FC. LR presented statistically significant difference compared with RN, which had the lowest mean. Vickers hardness showed LD with the highest average, and no statistical difference was found between FC and LR. RN presented the lowest average. Fracture toughness showed FC and LR not statistically different from each other, likewise LD and RN. The brittleness index, considered also as the machinability of a material, showed within this study as positively dependent on Vickers hardness, which leads to conclusion that hardness of ceramics is related to its milling capacity. In addition, fracture toughness of pre-sintered ceramics is compared to polymer-based materials.

Polymers/chemistry , Ceramics/chemistry , Computer-Aided Design , Resin Cements/chemistry , Dental Porcelain/chemistry , Aluminum Silicates/chemistry , Reference Values , Surface Properties , Materials Testing , Reproducibility of Results , Analysis of Variance , Statistics, Nonparametric , Hardness Tests
Braz. j. med. biol. res ; 52(5): e8499, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001530


Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2 as the foundational building block. In addition, the Cell Counting Kit-8 assay was conducted to evaluate the anti-proliferation activity of compounds 1 and 2 against human spinal tumor cells OPM-2. The cell viability curves showed that the two compounds have anti-proliferation activity on spinal tumor cells, and the activity of compound 1 is higher than compound 2. The annexin V-FITC/PI assay and western blot were used to detect the apoptotic percentage of OPM-2 cells incubated with compounds 1 and 2. The YAP protein expression and its role in cell apoptosis were further studied with qRT-PCR, immunoblotting, and flow cytometer.

Humans , Polymers/chemistry , Cell Survival/drug effects , Apoptosis/drug effects , Caspases/metabolism , Cell Proliferation/drug effects , Ligands , Spinal Neoplasms/enzymology , Spinal Neoplasms/pathology , Transfection , Reverse Transcriptase Polymerase Chain Reaction , Cell Line, Tumor
Int. j. morphol ; 36(4): 1463-1471, Dec. 2018. graf
Article in Spanish | LILACS | ID: biblio-975723


Los materiales poliméricos han sido ampliamente investigados para aplicaciones biomédicas, teniendo especial relevancia cuando se encuentran en forma de micro- y nano-partículas. Últimamente se ha ampliado su campo de aplicación al ser conjugados con péptidos y ácidos nucleicos, por lo tanto, el interés en el estudio de este tipo de materiales, así como también en la formulación de nanoestructuras funcionalizadas como materiales, dispositivos y vehículos de transporte de agentes terapéuticos ha aumentado. Las recientes investigaciones en nanosistemas se inspiran en fenómenos naturales que estimulan la integración de señales moleculares y la mimetización de procesos a nivel celular, de tejidos y órganos. Tecnológicamente, la capacidad de obtener nanoestructuras esféricas mediante la combinación de materiales que presenten propiedades distintas a las que ningún otro material individual posee por sí solo, es lo que hace que las nanocápsulas sean particularmente atractivas. Las potenciales ventajas de los sistemas de nanopartículas de tipo polimérico se destacan a lo largo de cada parte de este artículo de revisión. El presente artículo aborda los aspectos más relevantes sobre la estructura, composición y algunos métodos de elaboración de los sistemas nanoparticulados. Además, expone algunos de los trabajos más recientes, centrados en sistemas de nanopartículas basados en polímeros dirigidos a la administración de agentes, publicados en artículos especializados de investigación y revisiones durante los últimos años.

Polymeric materials have been extensively investigated for biomedical applications including micro- and nanoparticles. Modern advances have broadened horizons for application with peptides and nucleic acids. Therefore, interests increased in the formulation of materials, devices and vehicles for transporting therapeutic agents in functionalized nanostructures. Recent nano-systems are inspired by natural phenomena that stimulate the integration of molecular signals and the mimicking of natural cellular processes, at tissue and organ levels. Technologically, the ability to obtain spherical nanostructures, which combine different properties, that no other single material possesses on its own, makes nanocapsules particularly attractive. Potential advantages over polymer nanoparticulate systems are highlighted throughout each part of this review article. Here, we address the most relevant aspects of structure, composition and methods of formulation of nanoparticulate systems. In addition, we outline some of the more recent works focusing on nanosized preparations, based on agent-directed polymers, found in specialized research articles that have emerged in the recent years.

Polymers/chemistry , Nanoparticles/chemistry , Drug Delivery Systems , Tissue Engineering , Quantum Dots , Nanocapsules/chemistry , Nanospheres/chemistry
Int. j. morphol ; 36(4): 1490-1499, Dec. 2018. tab, graf
Article in Spanish | LILACS | ID: biblio-975727


Nanotecnología es la ciencia que involucra la síntesis de materiales en escala entre 1-100 nm (nanomateriales) es aplicable en diferentes áreas tales como medio ambiente, electrónica, alimentos, energía, entre otros. Los campos que serán relevantes dentro de esta revisión y explicados en detalle son la nanomedicina y la nano-odontología. Actualmente, en estas áreas los tres principales temas en desarrollo son específicamente en el sub-área de la nanobiotecnología y corresponden a: sensorización (biosensores/biodetección), diagnóstico (biomarcadores/bioimagen) y transportes de genes, proteínas o fármacos (sistemas de intercambio controlado en blancos sistémicos versus localizados). También se han presentado avances en bioaplicaciones como modelamientos de membranas, marcaje celular, entrega de agentes a blancos específicos, estrategias para prevención de enfermedades, ingeniería de tejidos, regeneración de órganos, estrategias de inmunoensayos y nano-oncología. Este artículo de revisión pretende abordar algunos de los aportes más relevantes, que tienen algunos de los trabajos recientes, sobre los sistemas de nanopartículas, principalmente aquellos dirigidos a terapias en áreas como diabetes, nano-oncología, terapia de fármacos y genes, mediante la técnica layer-by-layer y autoensamblado, muy utilizados también en ingeniería de tejidos y regeneración tisular, junto a un breve resumen de los avances que existen en el campo de la nano-odontología.

Nanotechnology is the science that involves the synthesis of materials in scale between 1-100 nm (nanomaterials) and is applicable in different areas such as environment, electronics, food, energy, among others. The fields that will be relevant within this review and explained in detail are nanomedicine and nano-dentistry. Currently, in these areas, the three main topics under development are specifically in the sub-area of nanobiotechnology and correspond to: sensorization (biosensors / biosensing), diagnostics (biomarkers / bioimaging) and transport of genes, proteins or drugs (exchange systems) controlled in systemic versus localized targets). Advances have also been presented in bioapplications such as membrane modeling, cell marking, delivery of agents to specific targets, strategies for disease prevention, tissue engineering, organ regeneration, immunoassay strategies and nano-oncology. This review article aims to address some of the most relevant contributions, some of the recent work, on nanoparticle systems, mainly those aimed at therapies in areas such as diabetes, nanooncology, drug and gene therapy, through the layer-by-layer and self-assembled technique, also widely used in tissue engineering and tissue regeneration, together with a brief summary of the advances that exist in the field of nano-dentistry.

Nanomedicine/trends , Polymers/chemistry , Bone Regeneration , Biosensing Techniques , Genetic Therapy , Drug Delivery Systems , Tissue Engineering , Nanotechnology , Dentistry/trends , Quantum Dots , Medical Oncology/trends
Braz. oral res. (Online) ; 32: e28, 2018. tab, graf
Article in English | LILACS | ID: biblio-889483


Abstract: The effects of several ceramic surface treatments on bond strength of a polymer-infiltrated ceramic network and resin composite as repair material were evaluated. CAD-CAM blocks of a polymer-infiltrated ceramic network (Vita Enamic) were sliced and subjected to aging process, followed by embedding in acrylic resin. The bonding/repair area was treated as follows (n = 30): C- without treatment; UA- universal adhesive application; FM- 10% hydrofluoric acid and silane application; OM-airborne-particle abrasion with aluminum oxide and silane application; RP- tribochemical silica coating; and CA- surface grinding and application of universal adhesive. Composite resin cylinders were made on the treated surface. Specimens from each group were assigned randomly to two subgroups (n = 15) considering storage condition: Baseline (shear tests after 48 hours) or Storage (tests after 6 months under distilled water). The treated surfaces were analyzed by goniometry, roughness, and SEM. Two-way ANOVA and 1-way ANOVA were applied to analyze the bond data and roughness / contact angle data, respectively, followed by Tukey's test (α = 5%). Surface treatments and storage conditions affected bond strengths (p < 0.01). Surface grinding (CA) followed by universal adhesive promoted the highest value of bond strength (14.5 ± 4.8 MPa for baseline, 8.5 ± 3.4 MPa for storage) and the roughest ceramic surface. Grinding with silicon carbide paper (simulating diamond bur) followed by the application of a universal adhesive system is the best option for repairing fractures of the polymer-infiltrated ceramic network.

Ceramics/chemistry , Composite Resins/chemistry , Dental Bonding/methods , Polymers/chemistry , Analysis of Variance , Computer-Aided Design , Dental Restoration Failure , Materials Testing , Microscopy, Electron, Scanning , Reference Values , Reproducibility of Results , Shear Strength/drug effects , Surface Properties/drug effects , Time Factors
Braz. oral res. (Online) ; 31: e92, 2017. tab, graf
Article in English | LILACS | ID: biblio-952127


Abstract The development of a biodegradable material with antimicrobial properties for local applications is required in the prevention and treatment of infectious diseases. The objective of this study was to produce blends of poly-L-lactide acid (PLLA) synthetic polymer associated with several antimicrobials, as an alternative in the prevention and treatment of infections, as well as to evaluate its cytotoxicity, release of antimicrobials and inhibit bacteria growth. Blends of PLLA added with 20% Amoxicillin, Metronidazole, Clindamycin or Azithromicyn were used to produce Films (F) or Meshs (M) by casting and electrospinning methods, respectively. Standardized discs of the films and meshs were stored in buffer solutions (pH 5 or 7.4) and aliquots were analyzed by high performance chromatography (HPLC) during 168 hours. Cytotoxicity on human gingival fibroblasts was tested after 24, 48 and 72h by MTT reaction. The antimicrobial capacity was determined against P. gingivalis and S. pyogenes. The specimens were weighed after 3 and 6 months of storage for degradation analysis. SEM was performed to control interfaces and degradation. Antimicrobials presented a continuous and exponential drug release. Analysis showed that both M and F were able to inhibit S. pyogenes and P. gingivalis growth, indicating the release of active antimicrobial agents. The products were not toxic to the fibroblasts. Amoxicillin-film showed more degradation than PLLA at both pHs (p < 0.05), whereas Azithromycin-meshes were more degraded than PLLA at pH 7.4 (p < 0.05). PLLA association with antimicrobials is biocompatible and may represent a potential tool for the local delivery of antimicrobials.

Humans , Polyesters/pharmacology , Polymers/pharmacology , Streptococcus pyogenes/drug effects , Biocompatible Materials/pharmacology , Porphyromonas gingivalis/drug effects , Microbial Viability/drug effects , Anti-Infective Agents/pharmacology , Polymers/chemistry , Surgical Mesh/adverse effects , Biocompatible Materials/chemistry , Materials Testing , Cell Culture Techniques , Drug Combinations , Anti-Infective Agents/chemistry
Braz. oral res. (Online) ; 30(1): e120, 2016. tab, graf
Article in English | LILACS | ID: biblio-951977


Abstract This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. Mesenchymal stem cell medium was used in the control groups. DPSC were transplanted onto the backs of mice via three scaffolds: copolymer of L-lactide and DL-lactide (PLDL), copolymer of DL-lactide (PDL) and hydroxyapatite tricalcium phosphate (HA/TCP). The expression levels of dentin sialo-phosphoprotein (DSPP), dentin matrix protein-1 (DMP1), enamelysin/matrix metalloproteinase 20 (MMP20) and phosphate-regulating gene with homologies to endopeptidases on X chromosome (PHEX) were analysed using RT-PCR. The expressions in the experimental groups were compared to those in the control groups. The transcript expressions at 6 and 12 weeks were significantly different for all scaffolds (p < 0.05), except for the expression of DSPP in the PLDL group with regard to the time variable. Although there was a decrease in the expression of enamelysin/MMP20 in PLDL and HA/TCP at 12 weeks, all other expressions increased and reached their highest level at 12 weeks. The highest DSPP expression was in the PDL group (p < 0.05). The highest expression of DMP1 was detected in the HA/TCP group (p < 0.05). The highest expression of PHEX was in the PLDL group (p < 0.05). Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation.

Humans , Animals , Polymers/chemistry , Stem Cells/physiology , Cell Differentiation/physiology , Dental Pulp/cytology , Tissue Scaffolds/chemistry , Phosphoproteins/analysis , Sialoglycoproteins/analysis , Time Factors , Biocompatible Materials/chemistry , Calcium Phosphates/chemistry , Gene Expression , Reproducibility of Results , Extracellular Matrix Proteins/analysis , Durapatite/chemistry , Cell Culture Techniques , Dental Enamel/chemistry , Dentin/chemistry , Dioxanes/chemistry , Matrix Metalloproteinase 20/analysis , PHEX Phosphate Regulating Neutral Endopeptidase/analysis
São Paulo; s.n; 2016. 109 p. ilus, tab. (BR).
Thesis in Portuguese | LILACS, BBO | ID: biblio-867995


As próteses faciais e intraorais tem um importante papel na devolução da estética e de algumas funções para os pacientes. Por meio da restauração da imagem corporal é possível reintegrá-lo a sociedade, resgatando assim a identidade do indivíduo. A boa condição dessas próteses é primordial para que estas possam exercer suas funções adequadamente e manter o local, onde estão inseridas, livre de infecções e inflamações. Portanto, a não formação de colônias e biofilmes bacterianos em materiais eleitos para confecção dessas próteses, trarão benefícios aos pacientes reabilitados. Visando isso, a presente dissertação verificou a capacidade de inclusão e a eficiência bactericida do biocida policloreto de dialildimetilamônio (PDADMAC) em resina acrílica autopolimerizável (RAAQ) e termopolimerizável (RAAT), e silicone de uso médico. Os resultados mostraram que o biocida PDADMAC quando dissolvido no tetrahidrofurano apresentou boa incorporação tanto nas resinas acrílicas, quimicamente ativas e termo ativas, quanto no silicone de uso médico e que apenas os corpos de prova que receberam 2 mililitros do PDADMAC em massa polimérica tiveram uma resposta bactericida eficaz.

The facial and intraoral prosthesis has an important role in the aesthetics and return of some functions to patients. Through restoration of the body image can reistante to can society , thus recovering the individual's identity . The good condition of these prostheses is essential so they can perform their function properly and maintain the the area where the prostheses are inserted free of infection and inflammation. Therefore, no formation of bacteria colonies and biofilms in the chosen materials for making these prostheses , will bring benefits to patients rehabilitated. The present work evaluated the capability of inclusion and the bactericidal efficiency of the biocide poly(diallyldimethylammonium chloride) ( pDADMAC ) of acrylic resin autopolymmerized ( RAAQ ) and thermal polymerized ( RAAT ) , and silicone medical use. The results showed that the biocide pDADMAC when dissolved in tetrahydrofuran presented a good incorporation in both acrylic resins and in the medical grade silicone and that only the samples that received 2 ml of pDADMAC in polymer had an effective bactericidal response.

Humans , Male , Female , Silicone Elastomers/analysis , Silicone Elastomers , Silicone Elastomers/adverse effects , Silicone Elastomers/chemical synthesis , Materials/analysis , Materials/methods , Materials/prevention & control , Polymers/analysis , Polymers/adverse effects , Polymers/chemistry
Rev. bras. epidemiol ; 18(supl.2): 68-82, Out.-Dez. 2015. tab
Article in English | LILACS | ID: lil-776716


RESUMO: Objetivo: Descrever os principais resultados dos fatores de risco e proteção de doenças crônicas não transmissíveis (DCNT), nas capitais brasileiras e no Distrito Federal, coletados pela Pesquisa Nacional de Saúde (PNS) e pelo Sistema de Vigilância de Fatores de Risco e Proteção para Doenças Crônicas Não Transmissíveis por Inquérito Telefônico (Vigitel) no ano de 2013. Métodos Análise de dados advindos da PNS e do Vigitel, pesquisas realizadas no ano de 2013. Foram analisados indicadores sobre tabagismo, consumo de álcool, alimentação e atividade física, segundo sexo, com intervalo de confiança de 95% (IC95%). Resultados: As prevalências encontradas foram: fumantes atuais de cigarro - PNS 12,5% e Vigitel 11,3%; consumo abusivo de bebida alcoólica - PNS 14,9% e Vigitel 16,4%; consumo recomendado de frutas e hortaliças - PNS 41,8% e Vigitel 23,6%; prática de atividade física no tempo livre - PNS 26,6% e Vigitel 33,8%. Conclusão: Os resultados dos indicadores foram semelhantes, em especial quando as perguntas e opções de resposta também eram. As pesquisas são úteis para o monitoramento dos fatores de risco e proteção das DCNT, podendo apoiar programas de promoção da saúde.

ABSTRACT: Objective: To describe risk and protective factors for chronic diseases, in Brazilian capitals and the Federal District, collected by the National Health Survey (PNS) and by the Surveillance System for Protective and Risk Factors for Chronic Diseases by Telephone Survey (Vigitel) in 2013. Methods: Data analysis of the studies conducted by the PNS and Vigitel in 2013 was performed. Indicators analyzed were: smoking, alcohol consumption, diet, and physical activity, according to sex, with a 95% confidence interval. Results: The prevalences found were: current cigarette smokers: PNS, 12.5% and Vigitel, 11.3%; abuse of alcoholic beverages: PNS, 14.9% and Vigitel, 16.4%; recommended intake of fruits and vegetables: PNS, 41.8% and Vigitel, 23.6%; and physical activity in leisure time: PNS, 26.6% and Vigitel, 35.8%. Conclusion: In the majority of indicators, the results were similar, especially when the questions and response options were equal. Surveys are useful for the monitoring of risk and protective factors of noncommunicable diseases and can support health promotion programs.

Polymers/chemistry , Gramicidin/administration & dosage , Ions , Microscopy, Electron, Transmission , Permeability
Braz. oral res. (Online) ; 29(1): 1-6, 2015. tab, ilus
Article in English | LILACS | ID: lil-777218


The aim of this study was to evaluate the ability of gutta-percha and a thermoplastic synthetic polymer (Resilon) to fill simulated lateral canals, using warm vertical compaction. Forty-five single-rooted human teeth were prepared using the rotary crown-down technique. Artificial lateral canals were made at 2, 5, and 8 mm from the working length (WL) in each root. The specimens were divided into three groups (n = 15), according to the filling material: Dentsply gutta-percha (GD), Odous gutta-percha (GO), and Resilon cones (RE). The root canals were obturated using warm vertical compaction, without endodontic sealer. The specimens were subjected to a tooth decalcification and clearing procedure. Filling of the lateral canals was analyzed by digital radiography and digital photographs, using the Image Tool software. The data were subjected to the Kruskal-Wallis and Dunn tests at 5% significance. RE had the best filling ability in all root thirds (p < 0.05), with similar results for GO in the coronal third. In the middle and apical thirds, GD and GO had similar results (p > 0.05). Resilon may be used as an alternative to gutta-percha as a solid core filling material for use with the warm vertical compaction technique. The study findings point to the potential benefit of the warm vertical compaction technique for filling lateral canals, and the study provides further information about using Resilon and gutta-percha as materials for the warm vertical compaction technique.

Humans , Polymers/chemistry , Root Canal Filling Materials/chemistry , Root Canal Obturation/methods , Gutta-Percha/chemistry , Root Canal Obturation/instrumentation , Materials Testing , Reproducibility of Results , Statistics, Nonparametric , Radiography, Dental, Digital , Hot Temperature
Int. j. morphol ; 32(3): 767-772, Sept. 2014. ilus
Article in Spanish | LILACS | ID: lil-728264


La ingeniería tisular se plantea como tratamiento ideal para la regeneración de tejidos con la utilización de andamiajes, células madres y factores de crecimiento. Las células madres de origen gingival plantean ventajas de obtención, mientras que el OPLA 3D permite cultivos de alta densidad celular. El objetivo de esta investigación fue evaluar la biocompatibilidad de células madres de origen gingival en OPLA. Las células se obtuvieron de tejido gingival y fueron caracterizadas fenotípica y funcionalmente. La biocompatibilidad se evaluó mediante la proliferación celular, prueba de viabilidad con azul tripán y diferenciación celular a linaje condrogénico y osteogénico. El recultivo del constructo se utilizó para evaluar la capacidad de transporte. Las células al interior del OPLA se visualizaron mediante cortes teñidos con H-E. Las células madres mesenquimales en OPLA proliferaron, 80% de confluencia a la cuarta semana. La viabilidad celular en OPLA fue de 83,32%. En el recultivo, las células comienzan a proliferar a la semana. El OPLA permite la diferenciación celular a linaje condrogénico y osteogénico. Se observan células al interior del OPLA, permite la proliferación, viabilidad y diferenciación celular. El OPLA podría ser utilizado como andamiaje celular para la ingeniería de tejidos.

Tissue engineering arises as the ideal treatment for tissue regeneration with the use of scaffolds, stem cells and growth factors. Stem cells derived from gingival tissue present benefits in its objection. 3D OPLA allow high cell density cultures. The objective of this study was to evaluate the biocompatibility of gingival stem cells in OPLA. Cells were obtained from gingival tissue and were characterized phenotypically and functionally. The biocompatibility was evaluated through cell proliferation, viability test with trypan blue and cell differentiation to chondrogenic and osteogenic lineage. Recultivation of the construct was used to evaluate transportability. Cells inside OPLA were visualized by stained sections with H&E. Mesenchymal stem cells proliferated in OPLA, 80% confluence at the fourth week. Cell viability in OPLA was 83.32%. In recultivation, cells start proliferating in a week. OPLA allows cell differentiation to chondrogenic and osteogenic lineage. Cells were observed within OPLA. In conclusion OPLA allows proliferation, viability and cell differentiation. OPLA could be used as scaffolds for cells in tissue engineering.

Humans , Tissue Engineering/methods , Mesenchymal Stem Cells/cytology , Gingiva/cytology , Osteogenesis , Polymers/chemistry , Materials Testing , Cell Differentiation , Cell Survival , Cells, Cultured , Immunophenotyping , Connective Tissue Cells/cytology , Lactic Acid/chemistry , Chondrogenesis , Cell Proliferation , Tissue Scaffolds
Rev. Asoc. Odontol. Argent ; 102(3): 108-114, sept. 2014. ilus, tab, graf
Article in Spanish | LILACS | ID: lil-731426


Objetivos: evaluar la adhesión cerámico-polímero en materiales usados en restauraciones odontológicas de alta resistencia (alúmina, zirconia) luego del tratamiento de la superficie cerámica. Materiales y métodos: se confeccionaron probetas con un cerámico a base de alúmina y sus superficies fueron modificadas a partir de vidriados y de la posterior formación de microencastres, mediante la sinterización de microesferas o de ataques químicos sobre una capa vítrea previamente sinterizada sobre ellas. Dos de las probetas fueron unidas mediante un adhesivo polimérico, a fin de determinar, bajo tensiones de corte, la resistencia y la tenacidad al despegue y el módulo de corte, en todos los casos, en megapascales y en un mínimo de 15 ensayos por condición experimental. Resultados: se obtuvieron valores medios de resistencia al despegue de entre 7 y 13 MPa; de tenacidad al despegue de entre 3 y 10 MPa, y de módulo de corte entre 5 y 13 MPa. Conclusión: la valoración del desempeño mecánico demuestra que las modificaciones superficiales de los cerámicos generan un comportamiento adhesivo mejorado respecto de lo que ocurre con cerámicos sin modificaciones

Ceramics/chemistry , Polymers/chemistry , Dental Bonding/methods , Composite Resins/chemistry , Surface Properties , Aluminum Oxide , Glass , Materials Testing , Shear Strength , Tensile Strength , Thermodynamics , Zirconium
Rev. bras. cir. cardiovasc ; 29(2): 272-278, Apr-Jun/2014. tab, graf
Article in English | LILACS | ID: lil-719402


Objective: The aim of the present investigation was to determine whether the difference in inflammatory tissue reaction between the Riccinus communis (castor) polymer with calcium carbonate and the titanium implant is statistically significant. Methods: Thirty-two Cavia porcellus were allocated into four groups of eight animals each. We implanted the two types of materials in the retroperitoneal space of all the animals. They were euthanized at 7, 20, 30 and 40 days after surgery, and an histological study of the samples was conducted. Results: All implants showed characteristics of chronic inflammation regardless of the material and timepoint of evaluation. There was no statistically significant difference between Pm+CaCO3 and Ti with regard to the presence of granulation tissue, tissue congestion, histiocytes, lymphocytes, neutrophils, giant cells, and fibrosis (P> 0.05). Conclusion: The castor oil polymer plus calcium carbonate implant was not statistically different from the titanium implant regarding inflammatory tissue reaction. .

Objetivo: Determinar se a reação tecidual do implante retroperitoneal do polímero de óleo de mamona com acréscimo de carbonato de cálcio (Pm+CaCO3) é significativa, por meio de análise histopatológica, tendo como controle o implante de titânio não tratado (Ti). Métodos: Estudo experimental, intervencionista e randomizado com 32 cobaias. Os animais foram separados em quatro grupos iguais e eutanasiados com 7, 20, 30 e 40 dias após o ato cirúrgico. Foram confeccionadas lâminas em hematoxilina-eosina e em tricrômio de Masson. Em relação a variáveis qualitativas dicotômicas, para análise da diferença entre o Pm+CaCO3 e o Ti em cada momento de avaliação foi usado o teste binomial. Considerando os materiais separadamente, a comparação dos quatro grupos foi feita utilizandose o teste exato de Fisher. Valores de P<0,05 indicaram significância estatística. Resultados: Todos os implantes apresentaram características de inflamação crônica, independente do material e do momento de avaliação. Não houve diferença estatisticamente significativa entre o Pm+CaCO3 e o Ti considerando a presença de tecido de granulação, congestão tecidual, histiócitos, linfócitos, neutrófilos, células gigantes e fibrose (P>0,05). Conclusão: Não foi encontrada diferença significativa entre a reação tecidual do Pm+CaCO3 e a do Ti. .

Animals , Guinea Pigs , Male , Calcium Carbonate/pharmacology , Castor Oil/pharmacology , Polymers/pharmacology , Ricinus/chemistry , Subcutaneous Tissue/drug effects , Titanium/pharmacology , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Calcium Carbonate/chemistry , Castor Oil/chemistry , Granulation Tissue/drug effects , Implants, Experimental , Materials Testing , Models, Animal , Prostheses and Implants , Polymers/chemistry , Random Allocation , Reproducibility of Results , Subcutaneous Tissue/pathology , Time Factors , Titanium/chemistry
Indian J Exp Biol ; 2014 Mar; 52(3): 252-260
Article in English | IMSEAR | ID: sea-150355


Activity differences of the first (phenylalanine ammonia lyase, PAL) and the last (cinnamyl alcohol dehydrogenase, CAD) enzymes of phenylpropanoid pathway in the roots of resistant (Yangambi Km5 and Anaikomban) and susceptible (Nendran and Robusta) banana cultivars caused by root lesion nematode, Pratylenchus coffeae, were investigated. Also, the accumulation of phenolics and deposition of lignin polymers in cell walls in relation to resistance of the banana cultivars to the nematode were analyzed. Compared to the susceptible cultivars, the resistant cultivars had constitutively significantly higher PAL activity and total soluble and cell wall-bound phenolics than in susceptible cultivars. The resistant cultivars responded strongly to the infection of the nematode by induction of several-time higher PAL and CAD enzymes activities, soluble and wall-bound phenolics and enrichment of lignin polymers in cell wall and these biochemical parameters reached maximum at 7th day postinoculation. In addition, profiles of phenolic acid metabolites in roots of Yangambi Km5 and Nendran were analyzed by HPLC to ascertain the underlying biochemical mechanism of bananas resistance to the nematode. Identification and quantification of soluble and cell wall-bound phenolic acids showed six metabolites and only quantitative, no qualitative, differences occurred between the resistant and susceptible cvs. and between constitutive and induced contents. A very prominent increase of p-coumaric, ferulic and sinapic acids, which are precursors of monolignols of lignin, in resistant cv. was found. These constitutive and induced biochemical alterations are definitely the chemical defenses of resistant cvs. to the nematode infection.

Alcohol Oxidoreductases/genetics , Alcohol Oxidoreductases/metabolism , Animals , Disease Resistance/genetics , Metabolic Networks and Pathways , Musa/enzymology , Musa/genetics , Musa/growth & development , Musa/parasitology , Nematoda/genetics , Nematoda/pathogenicity , Phenols/chemistry , Phenols/metabolism , Phenylalanine Ammonia-Lyase/genetics , Phenylalanine Ammonia-Lyase/metabolism , Plant Diseases/genetics , Plant Diseases/parasitology , Plant Roots/enzymology , Plant Roots/metabolism , Polymers/chemistry , Propanols/chemistry , Propanols/metabolism
Indian J Exp Biol ; 2013 Dec; 51(12): 1055-1062
Article in English | IMSEAR | ID: sea-150292


yielded an average particle size of 120 nm with 70% encapsulation-efficiency. In vitro release profile of NP-OP showed sustained release of OP for 21 days. In vivo anti-fertility studies were conducted in marmosets. Results indicated that control animals conceived in the same cycle while two of three treated animals failed to conceive in treatment cycle. The in vivo studies thus corroborate with in vitro release of OP, demonstrating its anti-fertility activity in 66% of animals.

Animals , Callithrix/physiology , Carrier Proteins/administration & dosage , Carrier Proteins/chemistry , Contraception , Female , Humans , Nanoparticles/administration & dosage , Nanoparticles/chemistry , Ovarian Follicle/chemistry , Particle Size , Peptide Fragments/administration & dosage , Peptide Fragments/chemistry , Polymers/administration & dosage , Polymers/chemistry
Indian J Exp Biol ; 2013 Nov; 51(11): 1012-1017
Article in English | IMSEAR | ID: sea-149411


Production of fuel ethanol from lignocellulosic biomass conventionally includes biomass pretreatment, hydrolysis, and fermentation. The liquor generated during dilute acid pretreatment of biomass contains considerable quantities of pentose sugars as well as various degradation products of sugars and lignin, like furfural, hydroxymethyl furfural (HMF), organic acids, aldehydes and others, which are known to be inhibitory for microbial growth. This pentose rich liquor is a potent resource which can be used to produce alcohol or other value added metabolites by microbial fermentation. However, the presence of these inhibitory compounds is a major hindrance and their removal is essential for efficient utilization of this byproduct stream. In the present work, the polymeric adsorbent resins, XAD-4, XAD-7 and XAD-16 were evaluated for their ability to adsorb fermentation inhibitors like furfural and HMF from the acid pretreated liquor. These resins could remove 55-75% of furfural and 100% of HMF and more than 90% sugar remained un-adsorbed in the pretreated liquor. Desorption of furfural from stationary phase was evaluated by using ethanol and hot water. The results suggest that these polymeric resins may be used for detoxification of acid pretreatment liquor with selective removal of sugar degradation products without affecting the sugar content in the solution.

Acids/chemistry , Adsorption , Biomass , Fermentation , Lignin/chemistry , Lignin/metabolism , Polymers/chemistry