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1.
Braz. j. biol ; 79(1): 53-62, Jan.-Mar 2019. tab, graf
Article in English | LILACS (Americas) | ID: biblio-984007

ABSTRACT

Abstract In this study, the activities of antioxidant enzymes, photosynthetic pigments, proline and carbohydrate contents in Pitcairnia encholirioides under ex vitro conditions of water deficit were evaluated. Results show that plants under progressive water stress, previously in vitro cultured in media supplemented with 30 g L-1 sucrose and GA3, accumulated more proline and increased peroxidase (POD) activity and the contents of photosynthetic pigments and carbohydrates. For plants previously in vitro cultured with 15 g L-1 sucrose and NAA, no differences were found for proline content and there were reductions in activities of peroxidase (POD), catalase (CAT) and poliphenoloxidase (PPO), and in contents of carbohydrates, with progress of ex vitro water deficit. After rehydration, plants showed physiological recovery, with enzymatic activities and contents of metabolites similar to those found in the controls not submitted to dehydration, regardless of the previous in vitro culture conditions. These results show that micropropagated P. encholirioides has high tolerance to dehydration once in ex vitro conditions, which can ensure the survival of plants from tissue culture when transferred to its natural environment, emphasizing the importance of such biotechnology for the propagation of endangered species.


Resumo Neste estudo, foram avaliadas as atividades de enzimas antioxidantes, pigmentos fotossintéticos, conteúdo de prolina e carboidratos em Pitcairnia encholirioides sob déficit hídrico em condições ex vitro. Os resultados mostraram que as plantas sob estresse hídrico progressivo, previamente cultivadas in vitro em meio de cultura suplementado com 30 g L-1 de sacarose e GA3 acumularam mais prolina e aumentaram a atividade da peroxidase (POD) e os teores de pigmentos fotossintéticos e carboidratos. Para plantas previamente cultivadas in vitro com 15 g L-1 de sacarose e ANA, não foram encontradas diferenças nos conteúdos de prolina e houve reduções nas atividades da peroxidase (POD), catalase (CAT) e polifenoloxidase (PPO), e no conteúdo de carboidratos, com o progresso do déficit hídrico ex vitro. Após a reidratação, as plantas apresentaram recuperação fisiológica, com atividades enzimáticas e conteúdo de metabólitos semelhantes aos encontrados nos controles não sujeitos à desidratação, independentemente das condições de cultivo in vitro. Estes resultados mostram que P. encholirioides micropropagada tem alta tolerância à desidratação uma vez em condições ex vitro, o que pode garantir a sobrevivência de plantas provenientes da cultura de tecidos quando transferidas para seu ambiente natural, enfatizando a importância desta biotecnologia para a propagação de espécies ameaçadas.


Subject(s)
Photosynthesis/physiology , Proline/metabolism , Water/metabolism , Bromeliaceae/physiology , Droughts , Antioxidants/metabolism , Pigments, Biological , Plant Proteins/metabolism , Bromeliaceae/enzymology , Carbohydrate Metabolism , Longevity
2.
Braz. j. biol ; 76(1): 218-227, Feb. 2016. tab, graf
Article in English | LILACS (Americas) | ID: lil-774510

ABSTRACT

Abstract This study aimed to evaluate the variation in the levels of proline, oxidative metabolism and photosynthetic pigments in plants of Pitcairnia encholirioides grown in vitro under different conditions and after acclimatization. The analyses were performed after 150 days of in vitro cultivation in MS media supplemented with 10 µM GA3 or 0.2 µM NAA, sucrose at 15 or 30 g L–1, in test tubes which allowed gas exchange or in a hermetically sealed system, and 180 days after acclimatization. The in vitro maintenance in hermetically sealed flasks, with GA3 and 15 g L–1 sucrose had adverse metabolic effects, which was demonstrated by the lower proline and photosynthetic pigments accumulation and by the increase in antioxidant enzymes activities. After acclimatization, differences for proline and photosynthetic pigments were no longer found and the enzymatic activities ranged unevenly. The results suggest that the in vitro cultivation in media with 0.2 µM NAA and 30 g L–1 sucrose, in test tubes capped with closures which allowed gas exchange, is more suitable for micropropagation of P. encholirioides, providing a prolonged maintenance of in vitro cultures and plantlets with superior quality for ex vitro development.


Resumo Este trabalho objetivou avaliar a contribuição da prolina, do metabolismo oxidativo e dos pigmentos fotossintéticos na propagação in vitro e aclimatização de Pitcairnia encholirioides, uma bromélia criticamente ameaçada de extinção. As análises foram realizadas após 150 dias de cultivo in vitro em meio MS suplementado com 10 µM de GA3 ou 0,2 µM de ANA, 15 ou 30 g L–1 de sacarose, em tubos de ensaio que permitiam trocas gasosas ou em sistema hermeticamente vedado, e também 180 dias após aclimatização. A manutenção in vitro em frascos hermeticamente fechados, com GA3 e 15 g L–1 de sacarose apresentou efeito metabólico adverso, demonstrado pelo menor acúmulo de prolina e pigmentos fotossintéticos e também pelo aumento das atividades de enzimas antioxidantes. Após aclimatização, as diferenças para prolina e pigmentos fotossintéticos não foram mais encontradas e as atividades enzimáticas variaram de maneira desuniforme. Os resultados sugerem que o cultivo in vitro em meio com 0,2 µM de ANA e 30 g L–1 de sacarose, em tubos fechados com tampas que permitem trocas gasosas, é mais adequado para a micropropagação de P. encholirioides, proporcionando uma manutenção prolongada das culturas in vitro e plântulas com qualidade superior para o desenvolvimento ex vitro.


Subject(s)
Bromeliaceae/physiology , Photosynthesis , Proline/metabolism , Acclimatization , Bromeliaceae/growth & development , Endangered Species , Oxidation-Reduction
3.
Rev. latinoam. enferm ; 23(1): 20-27, Jan-Feb/2015. tab
Article in English | LILACS (Americas), BDENF | ID: lil-742030

ABSTRACT

OBJECTIVE: to evaluate the indexes and the main factors associated with non-adherence to medication treatment for systemic arterial hypertension between urban and rural areas. METHOD: analytical study based on an epidemiological survey with a sample of 247 hypertensive residents of rural and urban areas, with application of a socio-demographic and economic questionnaire, and treatment adherence assessment. The Pearson's Chi-square test was used and the odds ratio (OD) was calculated to analyze the factors related to non-adherence. RESULTS: the prevalence of non-adherence was 61.9% and it was higher in urban areas (63.4%). Factors significantly associated with non-adherence were: male gender (OR=1.95; 95% CI 1.08-3.50), age 20-59 years old (OR=2.51; 95% CI 1.44-4.39), low economic status (OR=1.95; 95% CI 1.09-3.47), alcohol consumption (OR=5.92, 95% CI 1.73-20.21), short time of hypertension diagnosis (OR=3.07; 95% CI 1.35-6.96) and not attending the health service for routine consultations (OR=2.45; 1.35-4.42). CONCLUSION: the socio-demographic/economic characteristics, lifestyle habits and how to relate to health services were the factors that presented association with non-adherence regardless of the place of residence. .


OBJETIVO: avaliar os índices e os principais fatores associados a não adesão ao tratamento medicamentoso da hipertensão arterial sistêmica, entre área urbana e rural. MÉTODO: estudo analítico baseado em inquérito epidemiológico, realizado com amostra de 247 hipertensos moradores das áreas rural e urbana, com aplicação de questionário sociodemográfico, econômico e avaliação da adesão. Foi utilizado o teste quiquadrado de Pearson e calculado o Odds Ratio (OD) para análise dos fatores relacionados a não adesão. RESULTADOS: a prevalência da não adesão foi de 61,9%, sendo maior na área urbana (63,4%). Os fatores que apresentaram associação estatisticamente significativa com a não adesão foram: gênero masculino (OR=1,95; IC95% 1,08-3,50), faixa etária entre 20 e 59 anos (OR=2,51; IC95% 1,44-4,39), baixa classe econômica (OR=1,95; IC95% 1,09-3,47), etilismo (OR=5,92; IC 95% 1,73-20,21), tempo curto de diagnóstico de hipertensão (OR=3,07; IC95% 1,35-6,96) e não procura pelo serviço de saúde para consultas de rotina (OR=2,45; 1,35-4,42). CONCLUSÃO: as características sociodemográficas, econômicas, hábitos de vida e o modo de relacionar-se com os serviços de saúde foram os fatores que apresentaram associação com a não adesão, independentemente do local de residência. .


OBJETIVO: evaluar los índices y los principales factores asociados a la no adhesión al tratamiento medicamentoso de la hipertensión arterial sistémica entre área urbana y rural. MÉTODO: estudio analítico basado en investigación epidemiológica desarrollada con una muestra de 247 hipertensos moradores del área rural y urbana, con aplicación de un cuestionario sociodemográfico, económico y evaluación de la adhesión. Fue utilizado la prueba chi-cuadrado de Pearson y calculado el odds ratio (OD) para análisis de los factores relacionados a la no adhesión. RESULTADOS: la prevalencia de la no adhesión correspondió a 61,9%, siendo mayor en el área urbana (63,4%). Los factores que mostraron asociación estadísticamente significativa con la no adhesión fueron: género masculino (OR=1,95; IC95% 1,08-3,50), rango de edad entre 20 a 59 años (OR=2,51; IC95% 1,44-4,39), clase económica baja (OR=1,95; IC95% 1,09-3,47), etilismo (OR=5,92; IC 95% 1,73-20,21), tiempo corto de diagnóstico de hipertensión (OR=3,07; IC95% 1,35-6,96) y no procurar el servicio de salud para consultas de rutina (OR=2,45; 1,35-4,42). CONCLUSIÓN: las características sociodemográficas/económicas, hábitos de vida y el modo de relacionar con los servicios de salud fueron los factores que mostraron asociación con la no adhesión independientemente del local de residencia. .


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Young Adult , Amino Acid Metabolism, Inborn Errors/complications , Genetic Predisposition to Disease/genetics , Proline Oxidase/deficiency , Schizophrenia , Vitamin D Deficiency/complications , Amino Acid Metabolism, Inborn Errors/blood , Fasting/blood , Models, Statistical , Mutation/genetics , Proline Oxidase/blood , Proline Oxidase/genetics , Proline/metabolism , Risk Factors , Schizophrenia/blood , Schizophrenia/etiology , Schizophrenia/genetics , Vitamin D/analogs & derivatives , Vitamin D/blood
4.
Indian J Biochem Biophys ; 2014 Oct; 51(5): 407-415
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-154272

ABSTRACT

The involvement of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity and contents of H2O2, malondialdehyde (MDA) and proline was investigated in determining salinity tolerance among seedlings of thirty chickpea (Cicer arietinum L.) genotypes having different pedigrees. Chickpea genotypes, including cultivars and advanced lines were grown for 7 days under control and salt stress (50 mM NaCl) conditions. The genotypes showed differential response to salt stress in terms of growth, DPPH radical scavenging activity and contents of H2O2, MDA and proline in seedlings. On the basis of seedling growth, the genotypes having better performance under stress conditions had reduced levels of H2O2 and MDA contents, but increased levels of proline and DPPH radical scavenging activity. Stress tolerance index for these parameters was also determined. Agglomerative hierarchal clustering by Pearson correlation coefficient grouped the genotypes into two major clusters — MC I and MC II. MC II and A1-1 sub-cluster of MC-I comprised mainly of genotypes that showed higher stress resistance levels for the respective parameters in comparison to genotypes in other sub-clusters. Thus, it is possible to identify salt-tolerant genotypes on the basis of above parameters without a field trial.


Subject(s)
Biphenyl Compounds/metabolism , Cicer/physiology , Free Radical Scavengers/metabolism , Hydrogen Peroxide/metabolism , Malondialdehyde/metabolism , Oxidative Stress/physiology , Picrates/metabolism , Proline/metabolism , Reactive Oxygen Species/metabolism , Salinity , Salt Tolerance/physiology , Seedlings/physiology
5.
J Environ Biol ; 2007 Jul; 28(3): 561-6
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-113142

ABSTRACT

The effect of copperchloride (CuCl2) on the level of chlorophyll (a+b), proline, protein and abscisic acid in sunflower (Helianthus annuus L.) seedlings were investigated Control and copper treated (0.4, 0.5 and 0.6 mM) seedlings were grown for ten days in Hoagland solution. Abscisic acid content was determined in root, shoot and leaf tissues of seedlings by HPLC. Copper stress caused significant increase of the abscisic acid contents in roots, shoots and leaves of seedlings. The increase was dependent on the copper salt concentration. Enhanced accumulation of proline in the leaves of seedlings exposed to copper was determined, as well as a decrease of chlorophyll (a+b) and total protein (p < 0.05 or p < 0.01). It was observed that the level of chlorophyll (a+b) and total protein (p < 0.05 or p < 0.01) remarkably decreased as copper concentration increased to 0.6 mM, although the levels of proline and abscisic acid in the leaves of plants were increased--a dose-depended behavior The same trends were also observed with the level of abscisic acid of stems and roots. Copper has dose- depended effects on chlorophyll, proline, protein and abscisic acid level of sunflower (Helianthus annuus L.) seedlings. Thus, we assumed that copper levels increase above some critical points seedling growth get negative effects. This assumption is in line with previous findings.


Subject(s)
Abscisic Acid/metabolism , Chlorophyll/metabolism , Copper/toxicity , Helianthus/drug effects , Plant Leaves/drug effects , Plant Proteins/metabolism , Proline/metabolism , Seedlings/drug effects
6.
J Environ Biol ; 2007 Apr; 28(2): 303-6
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-113277

ABSTRACT

The effect of mercury (Hg) on the biochemical parameters of Lycopersicon esculentum Mill leaf was studied. Application of mercuric chloride in varying concentrations (0.5, 1.0, 1.5 and 2.0 mM HgCl2 kg(-1) sand) caused significant reduction that went up to 89% and 72% chlorophyll a and chlorophyll b contents respectively (at flowering stage), 69% in carotenoid content, 64% in total soluble protein content and 91% in nitrate reductase activity (all at post-flowering stage). The amounts of nitrate and proline increased maximally (151% and 143% respectively) at the flowering stage, whereas total soluble sugar enhanced by 57% at the post-flowering stage. Changes observed in most of the parameters, were concentration dependent. Such studies seem to be able to discover suitable bioindicators of heavy metal pollution.


Subject(s)
Carbohydrate Metabolism/drug effects , Carotenoids/metabolism , Chlorophyll/metabolism , Lycopersicon esculentum/drug effects , Mercury/toxicity , Nitrate Reductase/metabolism , Nitrates/metabolism , Plant Leaves/drug effects , Plant Proteins/metabolism , Proline/metabolism , Soil Pollutants/toxicity
7.
J Biosci ; 2006 Jun; 31(2): 265-72
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-110940

ABSTRACT

In the parent Nostoc muscorum an active proline oxidase enzyme is required to assimilate exogenous proline as a fixed nitrogen source. Cyanobacterial mutants, resistant to growth inhibitory action of proline analogue L-azetidine-2-carboxylate (Ac-R), were deficient in proline oxidase activity, and were over-accumulators of proline. Proline over-accumulation, resulting either from mutational acquisition of the Ac-R phenotype, or from salinity-induced uptake of exogenous proline, confirmed enhanced salinity/osmotic tolerance in the mutant strain. The nitrogenase activity and photosynthetic O 2 evolution of the parent were sensitive to both salinity as well as osmotic stresses than of Ac-R mutant strain. In addition, the mutation to Ac-resistant phenotype showed no alteration in salinity inducible potassium transport system in the cyanobacterium.


Subject(s)
Azetidinecarboxylic Acid/metabolism , Bacterial Proteins/metabolism , Mutation , Nostoc muscorum/genetics , Organisms, Genetically Modified , Osmotic Pressure , Potassium/metabolism , Proline/metabolism , Proline Oxidase/metabolism , Salts/metabolism , Sucrose/metabolism
8.
J Environ Biol ; 2006 May; 27(2 Suppl): 441-8
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-113145

ABSTRACT

17-day-old bean plants (Phaseolus vulgaris cv. Strike) were used to analyze the effects of Co2+ and Zn2+ on the time course of proline, total protein, chlorophyll and abscisic acid (ABA) levels in leaves. Controls, Co2+ and Zn2+-treated plants were grown for 8 days in Hoagland solution. Samples were taken at 2 day intervals. Proline, chlorophyll (a+b) and total protein contents of 17 day old primary leaves were determined by a spectrophotometer. ABA contents in roots and leaves of the seedlings were measured by high-pressure liquid chromatography. The presence of Zn2+ and Co2+ significantly increased the ABA contents in roots and leaves (p < 0.05 or p < 0.01). The increase of the abscisic acid content in the leaves was related to the content of the roots. This was further substantiated by enhanced accumulation of proline in the leaves of seedlings exposed to zinc and cobalt. The contents of chlorophyll (a+b) and total protein decreased with the concentration of both metals (p < 0.05 or p < 0.01). Cobalt proved to be comparatively more toxic than zinc.


Subject(s)
Abscisic Acid/metabolism , Chlorophyll/metabolism , Cobalt/toxicity , Phaseolus/metabolism , Plant Proteins/metabolism , Proline/metabolism , Soil Pollutants/toxicity , Zinc/toxicity
9.
Indian J Exp Biol ; 2000 Nov; 38(11): 1117-23
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-56510

ABSTRACT

Total collagen content in heart decreased significantly till day 8 of dexamethasone (Dex: 2.5 mg/kg week; s.c. for 2 weeks) treatment and increased on withdrawal of Dex. Acid soluble collagen content in heart decreased till day 12 of Dex treatment, reached normal level on day 16 of Dex treatment and exhibited an increase thereafter. Pepsin solubilized fraction in heart also behaved similarly as the acid soluble fraction, but reached normal level on Dex withdrawal. The total collagen content and the acid soluble collagen in kidney decreased significantly throughout treatment as well as on Dex withdrawal whereas, the pepsin solubilized collagen fraction in kidney exhibited a significant increase from day 8 of Dex treatment and the level was maintained throughout the experiment. Incorporation of 14C-proline in both, heart and kidney was found to be reduced. Electrophoretic pattern of pepsin collagen solubilized fraction of heart and kidney revealed alterations in subunit composition and its types on Dex administration and withdrawal. Thus, administration of Dex induced alterations in the metabolism of collagen and on Dex withdrawal, the system slowly tended to attain normalcy.


Subject(s)
Animals , Collagen/metabolism , Dexamethasone/administration & dosage , Kidney/metabolism , Male , Myocardium/metabolism , Proline/metabolism , Rats , Rats, Wistar
10.
Indian J Exp Biol ; 2000 Feb; 38(2): 192-5
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-60045

ABSTRACT

Changes in biomass yield rates, cell membrane stability (CMS), malondialdehyde (MDA) content and in the levels of physiological stress markers such as proline and glycine betaine in two high yielding genotypes (S1 and ATP, salt tolerant and salt sensitive, respectively) of mulberry under NaCl salinity were studied. Biomass yield rates and CMS were significantly decreased in both the genotypes under stress conditions. Per cent of decrease in biomass yield rate and CMS was relatively less in S1 than in ATP. Salt stress results a significant increase in the accumulation of proline, by 6-fold in S1 and 4-fold in ATP. Glycine betaine content was also increased significantly in stressed plants. However, the per cent increase was more in S1 than in ATP. The level of lipid peroxidation as indicated by MDA formation was greater in ATP than in S1. These results clearly support the better salt tolerant nature of S1 compared to ATP genotype.


Subject(s)
Betaine/metabolism , Biomass , Cell Membrane/metabolism , Genotype , Malondialdehyde/metabolism , Proline/metabolism , Rosales/genetics , Sodium Chloride
11.
Indian J Biochem Biophys ; 1999 Jun; 36(3): 207-10
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-27127

ABSTRACT

Response of Lathyrus sativus plants to water stress showed that ABA responsive genes such as PLE 25, TAS 14 and RAB 17 are synthesized constitutively, the levels of which decline gradually with increase in water stress or ABA levels. Proline accumulation was highest in leaves (65-fold) followed by stem (56-fold), root (38-fold) and marginal increase in etiolated seedlings. Proline increase was also observed in plant parts not exposed to light.


Subject(s)
Fabaceae/genetics , Plants, Medicinal , Proline/metabolism , RNA, Messenger/metabolism , Water
12.
Indian J Biochem Biophys ; 1996 Dec; 33(6): 484-90
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-27220

ABSTRACT

Hydroxyproline rich glycoprotein (HRGP) gene is induced in both control and gamma irradiated potato tubers after wounding. The enhanced RNA synthesis in response to wounding correlated well with the accumulation of both HRGP gene transcripts and protein. Initially, the level of HRGP gene expression in gamma irradiated potatoes in response to wounding was 30% more than the corresponding controls. After post irradiation storage of 3-5 weeks, HRGP gene expression in response to wounding was significantly lower than the unirradiated samples. This low level of HRGP gene expression in irradiated potatoes was partially retrieved by 5 mM Ca2+ treatment. Prior treatment with trifluoperazine, a calcium channel blocker resulted in 35% reduction in wound induced HRGP gene expression in control potatoes, further providing evidence for the involvement of Ca2+ dependency for HRGP gene activation. A comparative study on in vivo protein phosphorylation induced by wounding in control and irradiated potatoes exhibited significant differences. A good correlation was observed in the modulation of phosphorylation and HRGP gene expression by Ca2+ in irradiated potatoes. Wound induced signal transduction system and subsequent Ca2+ dependent protein phosphorylation for the activation of HRGP gene is affected in potatoes after gamma irradiation, thus impairing the wound healing process adversely.


Subject(s)
Calcium/pharmacology , Gamma Rays , Gene Expression Regulation, Plant , Glycoproteins/genetics , Phosphorylation , Plant Proteins/genetics , Proline/metabolism , RNA, Messenger/genetics , Solanum tuberosum/genetics , Transcriptional Activation
13.
Indian J Biochem Biophys ; 1996 Oct; 33(5): 420-4
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-28896

ABSTRACT

Biochemical changes that accompany acquisition of miconazole resistance in a single step mutant of C. albicans 3153 were analyzed. Experiments show that resistance to this drug was associated with decrease in total lipids, phospholipids and sterol content. Fluorescence polarization studies with 1,6-diphenyl-1,3,5-hexatriene (DPH) showed decrease in polarization value (p) in resistant cells, thus indicating changes in membrane fluidity. Uptake of [3H] proline by intact cells revealed decrease in K(m) and Vmax of high affinity system (S1) of proline transport in cells resistant to miconazole. Results of this study suggest that membrane sensitivity of miconazole is determined by overall membrane organisation rather than by affinity of antifungal drug(s) for a single membrane component.


Subject(s)
Antifungal Agents/pharmacology , Candida albicans/drug effects , Drug Resistance, Microbial/genetics , Lipid Metabolism , Membrane Fluidity , Miconazole/pharmacology , Mutation , Proline/metabolism
14.
Egyptian Journal of Physiological Sciences. 1993; 17 (2): 271-285
in English | IMEMR (Eastern Mediterranean) | ID: emr-27722

ABSTRACT

The interactions between each of ABA; 2 ppm [growth inhibitor], alar; 500 ppm [growth retardant] and proline; 100 ppm [amino acid] and water stress were undertaken in a trial to find out the best means in overcoming the injurious effect of soil drought on red radish plant. Under drought condition, the different treatments mentioned above caused remarkable increases in soluble sugars [reducing sugars and sucrose], polysaccharides, total sugars, 2-oxo-acids, amino-N, total soluble-N, total-N and protein-N above those of the untreated stressed plants, except that ABA-treated leaves contained less amounts of sucrose, at the later two ages, as compared with the untreated stressed ones. Moreover, foliar spraying of the stressed plants with both alar and proline resulted in the accumulation of each of P, Mg, Ca, Fe, B and Cu, with the exception that alar reduced the Cu values below the control amounts. In response to ABA treatment, P, Fe and B levels were elevated while Mg, Ca and Cu levels were decreased as being compared with those of the stressed untreated plants. Alar was found to be, the most effective applied chemicals for alleviating the adverse effect of drought on most of the metabolic activities of red radish plant


Subject(s)
Growth Inhibitors , Metabolism , Proline/metabolism , Drug Interactions , Abscisic Acid/metabolism
15.
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-21863

ABSTRACT

Auxotypes and penicillin sensitivity of 102 strains of N. gonorrhoeae were studied. Ten distinct auxotypes on the basis of growth requirement to seven amino acids were observed. The commonest pattern seen was zero auxotype (33.3%), followed by auxotype requiring proline (18.6%). MIC of 72 (71.5%) strains ranged from < 0.003 IU/ml to 0.062 IU/ml of benzyl penicillin. MIC of the other 30 (29.5%) strains was found to be > or = 0.125 IU/ml, indicating penicillin resistance. None of the strains were penicillinase producers. A case of reinfection was also detected on the basis of change in auxotype pattern.


Subject(s)
Amino Acids/metabolism , Arginine/metabolism , Bacterial Typing Techniques , Drug Resistance, Microbial , Female , Humans , Male , Microbial Sensitivity Tests , Neisseria gonorrhoeae/classification , Proline/metabolism
16.
Indian J Exp Biol ; 1991 Jan; 29(1): 49-51
Article in English | IMSEAR (South-East Asia), GHL | ID: sea-55970

ABSTRACT

Water stress drastically decreased relative water content and total soluble protein whereas it increased free amino acids, proline and protease enzyme in P. americanum. Treatments with 6-furfuryl amino purine (0.50 mM) and cycocel (60 mM) maintained the levels of soluble protein contents but the applications of furfuryl amino purine decreased free amino acids and free proline under stress conditions.


Subject(s)
Adenine/analogs & derivatives , Chlormequat/pharmacology , Cytokinins/pharmacology , Disasters , Kinetin , Plant Proteins/drug effects , Plants/drug effects , Proline/metabolism
17.
Ciênc. cult. (Säo Paulo) ; 42(10/12): 772-9, out.-dez. 1990. ilus, tab
Article in Portuguese | LILACS (Americas) | ID: lil-122103

ABSTRACT

Estudaram-se os efeitos da remoçäo das glândulas salivares maiores sobre a incorporaçäo de 3H-prolina pelos ameloblastos secretores e odontoblastos de incisivos de camundongos. Animais sialoadenectomizados e controles foram injetados com 3H-prolina, sendo sacrificados e perfundidos 30 min, 2h, 12h e 24h após. Cortes de 1*m obtidos de incisivos inferiores incluídos em Polybed foram radioautografados. A distribuiçäo percentual de gräos de prata reduzida/100*m* sobre as referidas células, assim como em suas respectivas matrizes, foi sempre maior nos animais sialoadenectomizadas, nos diferentes intervalos de tempo. Duas hipóteses poderiam explicar esses resultados: 1. um aumento na biossíntese protéica provavelmente devida à falta do fator submandibular inibidor da insulina (SII) associada a um relativo aumento do pool de 3H-prolina devido à remoçäo das glândulas salivares; 2. um decrécimo na degradaçäo protéica devido ao hipotireoidismo que eventualmente ocorre nos animais sialoadenectomizados


Subject(s)
Animals , Male , Mice , Dental Enamel/metabolism , Dentin/metabolism , Salivary Glands/surgery , Proline/metabolism , Ameloblasts/cytology , Ameloblasts/metabolism , Autoradiography , Odontoblasts/cytology , Odontoblasts/metabolism , Silver Proteins/analysis , Time Factors
18.
Rev. argent. microbiol ; 22(1): 7-16, 1990. tab
Article in Spanish | LILACS (Americas) | ID: lil-95047

ABSTRACT

En Saccharomyces cerevisiae la entrada de L-leucina es mediada por la permeasa general de aminoácidos, GAP, y dos sistemas cinéticamente caracterizados, uno de alta afinidad y baja velocidad, S1, para concentraciones externas de L-leucina 0,05-01mM y otro de baja afinidad, alta velocidad, S2, para concentraciones externas 1.0mM. En células crecidas en medios suplementados con amonio, como única fuente de nitrógeno, los valores de entrada e incorporación son menores que en células crecidas en medios suplementados con L-prolina. En condiciones de represión de la GAP por iones amonio, la entrada de L-leucina es mediada por los sistemas S1 y S2. Los dos sistemas son parcialmente inhibidos por efecto de iones amonio. En condiciones de depresion de la GAP, por crecimiento en L-prolina, la entrada de L-leucina es mediada por los sistemas S1 y GAP, bajas concentraciones externas de L-leucina mediada por centraciones externas. El amonio inhibe en mayor extensión la entrada de L-leucina mediada por La GAP


Subject(s)
Ammonium Sulfate/pharmacology , Leucine/pharmacokinetics , Membrane Transport Proteins/antagonists & inhibitors , Fungal Proteins/antagonists & inhibitors , Saccharomyces cerevisiae/drug effects , Citrulline/pharmacokinetics , Kinetics , Membrane Transport Proteins/metabolism , Proline/metabolism , Fungal Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Biological Transport
19.
P. R. health sci. j ; 7(2): 101-3, aug. 1988. ilus
Article in English | LILACS (Americas) | ID: lil-69664

ABSTRACT

La l-prolina ejerce unos efectos fisiológicos y conductúales los cuales sugieren su posible función como neuromodulador en el cerebro de los mamíferos. Se ha caracterizado parcialmente el enlazamiento de l-prolina a sinaptosomas de cerebro del ratón. Análisis preliminar de la cinética demustra la presencia de por lo menos dos sitios de enlazamiento en el rango submicromolar y de un sitio en el rango nanomolar. Aunque son necesarios estudios más detallados encaminados a esclarecer el significado biológico del enlazamiento de prolina a sinaptosomas de cerebro del ratón, estos resultados sirven de apoyo adicional a la posible función de prolina omo neuromodulador


Subject(s)
Rats , Animals , Brain/metabolism , Carrier Proteins/metabolism , Proline/metabolism , Synaptosomes/metabolism , Mice, Inbred C57BL
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